Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015834

Physical Location & Seq

Genomic Coordinates Forward (+)

16058335 .. 16061924

Gene Structure 3590 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1180300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGGCCATTGATACAGTGACATCCTCCCCGCTGGGCCCACCGGCGACAGAGAAGGACGCCAAGGCGCTTCAGTTCATAGAGGAGACGACCAGGAACACGGACTCGGTGCAGGAGAGGGTTCTGAGCGAGATTCTGAGGCGTAACGCCGAGACCGAATACCTCAAACGGTTCGGGCTTGGCGGTGCCACCGACCGGGACTCGTTCAAGTCGAAAGTCCCAGTTGTTACATACGAGGATCTCCTCCCGGAGATCCAACGTATTGCTAACGGCGACCGTTCTCCCGTCTTGAGCTCTCATCCCATCTCTGAGTTTCTCACTAGTTCTGGGACTTCAGCTGGTGAAAGAAAATTGATGCCTACAATCCATGAAGAGTTGGACCGTCGTACACTACTATACAGCTTGCTTCAGCCAGTGATGAACCTTTACGTGCCGGACCTCGATAAGGGAAAGGGGCTCTACTTCTTGTTCATCAAGGCGGAGACCAAGACGCCGGGAGGGCTGGTGGCACGTCCGGTGCTGACAAGCTACTACAAGAGTGAGCACTTCAAAACCAGGCCATATGACCCTTACAATGTCTTAACCAGCCCGAACGAGGCCATTCTTTGCGCCGACTCATTCCAAAGTATGTACACCCAAATGGTCTGCGGCCTCATCATGCGCCAACAAGTCCTCCGAGTAGGTGCAGTGTTTGCCTCCGGCCTACTCAGAGCGATTCGGTTCCTCCAGCTCAACTGGCAACAACTGTCCCTTGACATACGCAACGGAACCCTAAACCCTAAAATCACCGACCCTTCACTCCGCAAGACAATGTCCGGTATATTGAAACCGGATCCCGAGCTAGCAGATTTCATCACAAAGGAGTGTTGTGAGGAGAATTGGGCAGGCATAATCACAAGGGTTTGGCCTAACACAAAGTATCTCGACGTGATTGTCACCGGTGCAATGGCACAGTACATTCCGTTGCTTGAATACTACAGCAATGGCTTGCCGATGGCGTGTACTATGTATGCGTCGTCCGAATGTTATTTCGGACTCAACCTCAAGCCAATGTGCAAGCCGTCCGAGGTATCTTACACAATTATGCCTAACATGGCGTACTTTGAGTTCATCCCGCACGACCCTTCCGCTCCTTCGCTGACCAAACACTCCCCTCCCCGCCTTGTCGACCTTGCTGATGTCGAAGTTGGGAAGAAGTACGAGCTTGTCATAACAACCTACGCTGGACTGTGTCGTTACCGGGTGGGAGATATTCTCGAGGTCACCGGTTTCCACAATGCTGCACCGCAGTTTAGATTTGTGAGGAGGAAGAACGTCTTGTTGAGCATTGACTCCGACAAAACGGATGAGGCTGAGCTGCAGAAGGCTATTGACAATGCCTCAGTGTTGTTGCGAGAATTCAACACGACCGTAGTTGAGTACACGAGCTATGCAGACACCAAGACAATCCCCGGGCATTATGTGATTTACTGGGAATTGTTGGTGAAGGACCCGGCGAATAATTCCCCAAGTCACGAGGTTCTAGACAAGTGTTGTTTGGCCATGGAGGAGTCGTTGAACTCGGTGTACAGACAAGGCAGGGTGGCAGACAACTCAATTGGACCACTTGAGATAAGGGTGGTGAAAAATGGCACATTTGAGGAGCTTATGGACTATGCAATCTCTAGAGGTGCGTCTATCAATCAGTACAAGGCACCAAGGTGTGTGAATTTCTCCCCCATCATGGAACTTCTCGACTCCAGGGTCGTTTCGGTGCATTTTAGCCCAAGTGCACCGCAGTGGACTCCAGAACGACGTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0010252 GO:0010279 GO:0016874 GO:0016879 GO:0016881 GO:0042221 GO:0042592 GO:0048878 GO:0050896 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

GH3

Protein Analysis

600

Amino Acids

67.47

Weight (kDa)

6.02

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	23 - 331	2.7e-121	GH3 family N-terminal domain
GH3_M	PF23571	358 - 435	3.3e-30	GH3 family central domain
GH3_C	PF23572	451 - 570	5.7e-39	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015834)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59500 AT2G23170 AT4G37390
fragaria_vesca	FvH4_1g16980
malus_domestica	MD02G1180300.v1.1 MD15G1290900.v1.1
prunus_persica	Prupe.6G226100_v2.0.a1
pyrus_communis	pycom02g14600 pycom15g25440
rosa_laevigata	RLG00000017554
rosa_multiflora	Rmu_sc0002945.1_g000004
rosa_roxburghii	Rroxscaffold_2G00136610
rosa_rugosa	Rorug02G0140200
rosa_samantha	Rh2CG196000
rosa_wichuraiana	Rw2G015090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	929, 1739
AatII	GACGTC	1 cut(s)	1795
AccB1I	GGYRCC	2 cut(s)	182, 1690
AccBSI	CCGCTC	1 cut(s)	1127
AccI	GTMKAC	1 cut(s)	1164
AclWI	GGATC	4 cut(s)	243, 244, 824, 837
AcoI	YGGCCR	2 cut(s)	3, 1536
AcsI	RAATTY	2 cut(s)	1394, 1705
AcuI	CTGAAG	3 cut(s)	53, 315, 389
AcyI	GRCGYC	3 cut(s)	57, 488, 1792
AfaI	GTAC	9 cut(s)	385, 629, 953, 1000, 1097, 1196, 1418, 1565, 1685
AfiI	CCNNNNNNNGG	4 cut(s)	192, 442, 592, 1720
AgeI	ACCGGT	2 cut(s)	935, 1262
AgsI	TTSAA	6 cut(s)	205, 547, 823, 968, 1399, 1555
AhlI	ACTAGT	1 cut(s)	317
AjiI	CACGTC	2 cut(s)	509, 925
AjnI	CCWGG	3 cut(s)	89, 551, 1736
AleI	CACNNNNGTG	2 cut(s)	1696, 1774
Alw21I	GWGCWC	3 cut(s)	293, 543, 1771
Alw26I	GTCTC	3 cut(s)	77, 143, 473
Alw44I	GTGCAC	1 cut(s)	1767
AlwI	GGATC	4 cut(s)	243, 244, 824, 837
Ama87I	CYCGRG	3 cut(s)	833, 1253, 1448
AoxI	GGCC	8 cut(s)	3, 34, 554, 594, 646, 697, 902, 1536
ApaI	GGGCCC	1 cut(s)	38
ApaLI	GTGCAC	1 cut(s)	1767
ApeKI	GCWGC	2 cut(s)	1277, 1354
ApoI	RAATTY	2 cut(s)	1394, 1705
AsiGI	ACCGGT	2 cut(s)	935, 1262
AspLEI	GCGC	3 cut(s)	67, 608, 660
AspS9I	GGNCC	6 cut(s)	34, 35, 376, 433, 1486, 1598
AsuC2I	CCSGG	7 cut(s)	194, 245, 492, 1238, 1449, 1450, 1490
AsuHPI	GGTGA	6 cut(s)	350, 775, 925, 1252, 1492, 1630
AsuNHI	GCTAGC	1 cut(s)	838
AvaI	CYCGRG	3 cut(s)	833, 1253, 1448
AvaII	GGWCC	4 cut(s)	376, 433, 1486, 1598
BaeGI	GKGCMC	2 cut(s)	38, 1771
BalI	TGGCCA	2 cut(s)	5, 1538
BamHI	GGATCC	1 cut(s)	829
BanI	GGYRCC	2 cut(s)	182, 1690
BanII	GRGCYC	3 cut(s)	38, 293, 456
BauI	CACGAG	2 cut(s)	1420, 1511
Bbv12I	GWGCWC	3 cut(s)	293, 543, 1771
BbvI	GCAGC	2 cut(s)	1264, 1341
BccI	CCATC	3 cut(s)	308, 985, 1724
BceAI	ACGGC	2 cut(s)	283, 1042
BciT130I	CCWGG	3 cut(s)	91, 553, 1738
BcnI	CCSGG	7 cut(s)	194, 245, 492, 1238, 1449, 1450, 1490
BcoDI	GTCTC	3 cut(s)	77, 143, 473
BcuI	ACTAGT	1 cut(s)	317
BfaI	CTAG	4 cut(s)	318, 839, 1520, 1662
BfmI	CTRYAG	2 cut(s)	973, 1355
BfoI	RGCGCY	1 cut(s)	68
BglI	GCCNNNNNGGC	1 cut(s)	496
BisI	GCNGC	3 cut(s)	646, 1278, 1355
BlpI	GCTNAGC	1 cut(s)	1350
BlsI	GCNGC	3 cut(s)	647, 1279, 1356
Bme18I	GGWCC	4 cut(s)	376, 433, 1486, 1598
BmeT110I	CYCGRG	3 cut(s)	833, 1253, 1448
BmgBI	CACGTC	2 cut(s)	509, 925
BmgT120I	GGNCC	6 cut(s)	34, 35, 376, 433, 1486, 1598
BmiI	GGNNCC	7 cut(s)	36, 184, 719, 766, 831, 1488, 1692
BmrI	ACTGGG	2 cut(s)	212, 1477
BmsI	GCATC	1 cut(s)	342
BmtI	GCTAGC	1 cut(s)	842
BmuI	ACTGGG	2 cut(s)	212, 1477
BplI	GAGNNNNNCTC	2 cut(s)	1236, 1268
BpmI	CTGGAG	3 cut(s)	707, 1720, 1767
Bpu1102I	GCTNAGC	1 cut(s)	1350
BpuEI	CTTGAG	3 cut(s)	307, 1025, 1625
BpuMI	CCSGG	7 cut(s)	194, 245, 492, 1238, 1449, 1450, 1490
BsaAI	YACGTR	1 cut(s)	427
BsaHI	GRCGYC	3 cut(s)	57, 488, 1792
BsaI	GGTCTC	2 cut(s)	143, 473
BsaJI	CCNNGG	7 cut(s)	60, 1062, 1447, 1448, 1539, 1694, 1737
BsaWI	WCCGGW	5 cut(s)	511, 812, 826, 935, 1262
BsaXI	ACNNNNNCTCC	6 cut(s)	486, 516, 654, 684, 1535, 1565
Bsc4I	CCNNNNNNNGG	4 cut(s)	192, 442, 592, 1720
Bse118I	RCCGGY	3 cut(s)	40, 935, 1262
Bse1I	ACTGG	4 cut(s)	218, 410, 737, 1472
Bse3DI	GCAATG	2 cut(s)	948, 985
BseBI	CCWGG	3 cut(s)	91, 553, 1738
BseDI	CCNNGG	7 cut(s)	60, 1062, 1447, 1448, 1539, 1694, 1737
BseGI	GGATG	4 cut(s)	20, 295, 1107, 1348
BseLI	CCNNNNNNNGG	4 cut(s)	192, 442, 592, 1720
BseMI	GCAATG	2 cut(s)	948, 985
BseMII	CTCAG	6 cut(s)	113, 125, 297, 718, 1341, 1392
BseNI	ACTGG	4 cut(s)	218, 410, 737, 1472
BseRI	GAGGAG	6 cut(s)	95, 230, 884, 1315, 1559, 1652
BseSI	GKGCMC	2 cut(s)	38, 1771
BseXI	GCAGC	2 cut(s)	1264, 1341
BseYI	CCCAGC	1 cut(s)	31
BsgI	GTGCAG	3 cut(s)	128, 702, 1263
Bsh1285I	CGRYCG	3 cut(s)	193, 274, 1407
BshFI	GGCC	8 cut(s)	5, 36, 556, 596, 648, 699, 904, 1538
BshNI	GGYRCC	2 cut(s)	182, 1690
BshTI	ACCGGT	2 cut(s)	935, 1262
BsiEI	CGRYCG	3 cut(s)	193, 274, 1407
BsiHKAI	GWGCWC	3 cut(s)	293, 543, 1771
BsiHKCI	CYCGRG	3 cut(s)	833, 1253, 1448
BslFI	GGGAC	4 cut(s)	200, 209, 340, 730
BslI	CCNNNNNNNGG	4 cut(s)	192, 442, 592, 1720
BsmAI	GTCTC	3 cut(s)	77, 143, 473
BsmBI	CGTCTC	1 cut(s)	77
BsmFI	GGGAC	4 cut(s)	200, 209, 340, 730
BsnI	GGCC	8 cut(s)	5, 36, 556, 596, 648, 699, 904, 1538
Bso31I	GGTCTC	2 cut(s)	143, 473
BsoBI	CYCGRG	3 cut(s)	833, 1253, 1448
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	5 cut(s)	38, 293, 456, 543, 1771
Bsp1407I	TGTACA	2 cut(s)	627, 1563
Bsp143I	GATC	3 cut(s)	235, 249, 829
Bsp1720I	GCTNAGC	1 cut(s)	1350
Bsp19I	CCATGG	1 cut(s)	1539
BspANI	GGCC	8 cut(s)	5, 36, 556, 596, 648, 699, 904, 1538
BspCNI	CTCAG	6 cut(s)	114, 126, 298, 717, 1342, 1391
BspLI	GGNNCC	7 cut(s)	36, 184, 719, 766, 831, 1488, 1692
BspMAI	CTGCAG	1 cut(s)	1359
BspOI	GCTAGC	1 cut(s)	842
BspPI	GGATC	4 cut(s)	243, 244, 824, 837
BspT107I	GGYRCC	2 cut(s)	182, 1690
BspTNI	GGTCTC	2 cut(s)	143, 473
BsrBI	CCGCTC	1 cut(s)	1127
BsrDI	GCAATG	2 cut(s)	948, 985
BsrFI	RCCGGY	3 cut(s)	40, 935, 1262
BsrGI	TGTACA	2 cut(s)	627, 1563
BsrI	ACTGG	4 cut(s)	218, 410, 737, 1472
BssAI	RCCGGY	3 cut(s)	40, 935, 1262
BssECI	CCNNGG	7 cut(s)	60, 1062, 1447, 1448, 1539, 1694, 1737
BssMI	GATC	3 cut(s)	235, 249, 829
BssNI	GRCGYC	3 cut(s)	57, 488, 1792
BssSI	CACGAG	2 cut(s)	1420, 1511
BssT1I	CCWWGG	3 cut(s)	60, 1539, 1694
Bst2BI	CACGAG	2 cut(s)	1420, 1511
Bst2UI	CCWGG	3 cut(s)	91, 553, 1738
Bst4CI	ACNGT	8 cut(s)	16, 168, 275, 380, 744, 951, 1227, 1408
Bst6I	CTCTTC	1 cut(s)	363
BstACI	GRCGYC	3 cut(s)	57, 488, 1792
BstAPI	GCANNNNNTGC	1 cut(s)	689
BstAUI	TGTACA	2 cut(s)	627, 1563
BstBAI	YACGTR	1 cut(s)	427
BstC8I	GCNNGC	5 cut(s)	401, 840, 883, 986, 1055
BstDEI	CTNAG	6 cut(s)	122, 134, 306, 704, 1350, 1378
BstDSI	CCRYGG	1 cut(s)	1539
BstEII	GGTNACC	1 cut(s)	1258
BstENI	CCTNNNNNAGG	1 cut(s)	440
BstF5I	GGATG	4 cut(s)	20, 295, 1107, 1348
BstH2I	RGCGCY	1 cut(s)	68
BstHHI	GCGC	3 cut(s)	67, 608, 660
BstKTI	GATC	3 cut(s)	238, 252, 832
BstMAI	GTCTC	3 cut(s)	77, 143, 473
BstMBI	GATC	3 cut(s)	235, 249, 829
BstMCI	CGRYCG	3 cut(s)	193, 274, 1407
BstMWI	GCNNNNNNNGC	3 cut(s)	496, 689, 733
BstNI	CCWGG	3 cut(s)	91, 553, 1738
BstPI	GGTNACC	1 cut(s)	1258
BstSFI	CTRYAG	2 cut(s)	973, 1355
BstSLI	GKGCMC	2 cut(s)	38, 1771
BstV1I	GCAGC	2 cut(s)	1264, 1341
BstX2I	RGATCY	3 cut(s)	235, 249, 829
BstYI	RGATCY	3 cut(s)	235, 249, 829
BsuRI	GGCC	8 cut(s)	5, 36, 556, 596, 648, 699, 904, 1538
BtgI	CCRYGG	1 cut(s)	1539
BtrI	CACGTC	2 cut(s)	509, 925
BtsCI	GGATG	4 cut(s)	20, 295, 1107, 1348
BtsI	GCAGTG	2 cut(s)	690, 1781
BtsIMutI	CAGTG	5 cut(s)	21, 417, 690, 1386, 1781
Cac8I	GCNNGC	5 cut(s)	401, 840, 883, 986, 1055
CfoI	GCGC	3 cut(s)	67, 608, 660
Cfr10I	RCCGGY	3 cut(s)	40, 935, 1262
Cfr13I	GGNCC	6 cut(s)	34, 35, 376, 433, 1486, 1598
Cfr9I	CCCGGG	1 cut(s)	1448
CseI	GACGC	4 cut(s)	65, 496, 999, 1659
Csp6I	GTAC	9 cut(s)	384, 628, 952, 999, 1096, 1195, 1417, 1564, 1684
CspAI	ACCGGT	2 cut(s)	935, 1262
CviAII	CATG	5 cut(s)	365, 655, 1090, 1540, 1720
CviQI	GTAC	9 cut(s)	384, 628, 952, 999, 1096, 1195, 1417, 1564, 1684
DdeI	CTNAG	6 cut(s)	122, 134, 306, 704, 1350, 1378
DpnI	GATC	3 cut(s)	237, 251, 831
DpnII	GATC	3 cut(s)	235, 249, 829
DrdI	GACNNNNNNGTC	2 cut(s)	929, 1739
DseDI	GACNNNNNNGTC	2 cut(s)	929, 1739
EaeI	YGGCCR	2 cut(s)	3, 1536
Eam1104I	CTCTTC	1 cut(s)	363
EarI	CTCTTC	1 cut(s)	363
EciI	GGCGGA	1 cut(s)	491
Ecl136II	GAGCTC	1 cut(s)	291
Eco130I	CCWWGG	3 cut(s)	60, 1539, 1694
Eco24I	GRGCYC	3 cut(s)	38, 293, 456
Eco31I	GGTCTC	2 cut(s)	143, 473
Eco47I	GGWCC	4 cut(s)	376, 433, 1486, 1598
Eco53kI	GAGCTC	1 cut(s)	291
Eco57I	CTGAAG	3 cut(s)	53, 315, 389
Eco88I	CYCGRG	3 cut(s)	833, 1253, 1448
Eco91I	GGTNACC	1 cut(s)	1258
EcoICRI	GAGCTC	1 cut(s)	291
EcoNI	CCTNNNNNAGG	1 cut(s)	440
EcoO109I	RGGNCCY	1 cut(s)	1486
EcoO65I	GGTNACC	1 cut(s)	1258
EcoRI	GAATTC	1 cut(s)	1394
EcoRII	CCWGG	3 cut(s)	89, 551, 1736
EcoT14I	CCWWGG	3 cut(s)	60, 1539, 1694
EcoT38I	GRGCYC	3 cut(s)	38, 293, 456
ErhI	CCWWGG	3 cut(s)	60, 1539, 1694
Esp3I	CGTCTC	1 cut(s)	77
FaeI	CATG	5 cut(s)	368, 658, 1093, 1543, 1723
FalI	AAGNNNNNCTT	2 cut(s)	1298, 1330
FaqI	GGGAC	4 cut(s)	200, 209, 340, 730
FatI	CATG	5 cut(s)	364, 654, 1089, 1539, 1719
FauI	CCCGC	3 cut(s)	36, 1119, 1163
FauNDI	CATATG	1 cut(s)	559
FblI	GTMKAC	1 cut(s)	1164
Fnu4HI	GCNGC	3 cut(s)	646, 1278, 1355
FokI	GGATG	4 cut(s)	7, 282, 1094, 1355
FriOI	GRGCYC	3 cut(s)	38, 293, 456
Fsp4HI	GCNGC	3 cut(s)	646, 1278, 1355
FspBI	CTAG	4 cut(s)	318, 839, 1520, 1662
GlaI	GCGC	3 cut(s)	66, 607, 659
GluI	GCNGC	3 cut(s)	646, 1278, 1355
GsaI	CCCAGC	1 cut(s)	35
GsuI	CTGGAG	3 cut(s)	707, 1720, 1767
HaeII	RGCGCY	1 cut(s)	68
HaeIII	GGCC	8 cut(s)	5, 36, 556, 596, 648, 699, 904, 1538
HgaI	GACGC	4 cut(s)	65, 496, 999, 1659
HhaI	GCGC	3 cut(s)	67, 608, 660
Hin1I	GRCGYC	3 cut(s)	57, 488, 1792
Hin1II	CATG	5 cut(s)	368, 658, 1093, 1543, 1723
Hin6I	GCGC	3 cut(s)	65, 606, 658
HinP1I	GCGC	3 cut(s)	65, 606, 658
HincII	GTYRAC	1 cut(s)	1165
HindII	GTYRAC	1 cut(s)	1165
HphI	GGTGA	6 cut(s)	350, 775, 925, 1252, 1492, 1630
Hpy166II	GTNNAC	8 cut(s)	386, 630, 999, 1165, 1419, 1564, 1769, 1779
Hpy188I	TCNGA	9 cut(s)	123, 135, 307, 674, 707, 1018, 1031, 1063, 1333
Hpy188III	TCNNGA	9 cut(s)	286, 833, 920, 1253, 1511, 1520, 1662, 1730, 1784
Hpy8I	GTNNAC	8 cut(s)	386, 630, 999, 1165, 1419, 1564, 1769, 1779
Hpy99I	CGWCG	4 cut(s)	384, 926, 1015, 1794
HpyAV	CCTTC	6 cut(s)	46, 801, 1131, 1140, 1354, 1477
HpyCH4III	ACNGT	8 cut(s)	16, 168, 275, 380, 744, 951, 1227, 1408
HpyCH4IV	ACGT	6 cut(s)	256, 426, 508, 924, 1311, 1792
HpyF10VI	GCNNNNNNNGC	3 cut(s)	496, 689, 733
HpyF3I	CTNAG	6 cut(s)	122, 134, 306, 704, 1350, 1378
HpySE526I	ACGT	6 cut(s)	256, 426, 508, 924, 1311, 1792
Hsp92I	GRCGYC	3 cut(s)	57, 488, 1792
Hsp92II	CATG	5 cut(s)	368, 658, 1093, 1543, 1723
HspAI	GCGC	3 cut(s)	65, 606, 658
Kzo9I	GATC	3 cut(s)	235, 249, 829
LmnI	GCTCC	2 cut(s)	1132, 1639
Lsp1109I	GCAGC	2 cut(s)	1264, 1341
LweI	GCATC	1 cut(s)	342
MaeI	CTAG	4 cut(s)	318, 839, 1520, 1662
MaeII	ACGT	6 cut(s)	256, 426, 508, 924, 1311, 1792
MaeIII	GTNAC	7 cut(s)	16, 140, 223, 931, 1232, 1258, 1508
MalI	GATC	3 cut(s)	237, 251, 831
MbiI	CCGCTC	1 cut(s)	1127
MboI	GATC	3 cut(s)	235, 249, 829
MboII	GAAGA	3 cut(s)	380, 1201, 1318
MfeI	CAATTG	1 cut(s)	1593
MflI	RGATCY	3 cut(s)	235, 249, 829
MhlI	GDGCHC	5 cut(s)	38, 293, 456, 543, 1771
MlsI	TGGCCA	2 cut(s)	5, 1538
MluCI	AATT	8 cut(s)	347, 874, 1077, 1394, 1472, 1498, 1593, 1705
MluNI	TGGCCA	2 cut(s)	5, 1538
MlyI	GAGTC	8 cut(s)	95, 191, 605, 1026, 1322, 1556, 1727, 1774
MmeI	TCCRAC	3 cut(s)	277, 354, 1356
Mox20I	TGGCCA	2 cut(s)	5, 1538
MscI	TGGCCA	2 cut(s)	5, 1538
MseI	TTAA	2 cut(s)	578, 1798
MslI	CAYNNNNRTG	4 cut(s)	635, 1079, 1696, 1774
Msp20I	TGGCCA	2 cut(s)	5, 1538
MspA1I	CMGCKG	2 cut(s)	31, 335
MunI	CAATTG	1 cut(s)	1593
MvaI	CCWGG	3 cut(s)	91, 553, 1738
MwoI	GCNNNNNNNGC	3 cut(s)	496, 689, 733
NciI	CCSGG	7 cut(s)	194, 245, 492, 1238, 1449, 1450, 1490
NcoI	CCATGG	1 cut(s)	1539
NdeI	CATATG	1 cut(s)	559
NdeII	GATC	3 cut(s)	235, 249, 829
NheI	GCTAGC	1 cut(s)	838
NlaIII	CATG	5 cut(s)	368, 658, 1093, 1543, 1723
NlaIV	GGNNCC	7 cut(s)	36, 184, 719, 766, 831, 1488, 1692
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	4 cut(s)	16, 931, 1258, 1508
OliI	CACNNNNGTG	2 cut(s)	1696, 1774
PaeR7I	CTCGAG	1 cut(s)	1253
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 206
PfeI	GAWTC	2 cut(s)	130, 712
PflFI	GACNNNGTC	2 cut(s)	808, 1227
PfoI	TCCNGGA	1 cut(s)	243
PinAI	ACCGGT	2 cut(s)	935, 1262
PkrI	GCNGC	3 cut(s)	647, 1279, 1356
PleI	GAGTC	8 cut(s)	95, 191, 605, 1026, 1322, 1555, 1727, 1774
PpsI	GAGTC	8 cut(s)	95, 191, 605, 1026, 1322, 1555, 1727, 1774
Ppu21I	YACGTR	1 cut(s)	427
PpuMI	RGGWCCY	1 cut(s)	1486
Psp124BI	GAGCTC	1 cut(s)	293
Psp5II	RGGWCCY	1 cut(s)	1486
Psp6I	CCWGG	3 cut(s)	89, 551, 1736
PspEI	GGTNACC	1 cut(s)	1258
PspFI	CCCAGC	1 cut(s)	31
PspGI	CCWGG	3 cut(s)	89, 551, 1736
PspN4I	GGNNCC	7 cut(s)	36, 184, 719, 766, 831, 1488, 1692
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	6 cut(s)	34, 35, 376, 433, 1486, 1598
PspPPI	RGGWCCY	1 cut(s)	1486
PsrI	GAACNNNNNNTAC	4 cut(s)	1088, 1120, 1547, 1579
PstI	CTGCAG	1 cut(s)	1359
PsuI	RGATCY	3 cut(s)	235, 249, 829
PsyI	GACNNNGTC	2 cut(s)	808, 1227
PvuII	CAGCTG	1 cut(s)	335
RsaI	GTAC	9 cut(s)	385, 629, 953, 1000, 1097, 1196, 1418, 1565, 1685
RsaNI	GTAC	9 cut(s)	384, 628, 952, 999, 1096, 1195, 1417, 1564, 1684
RseI	CAYNNNNRTG	4 cut(s)	635, 1079, 1696, 1774
SacI	GAGCTC	1 cut(s)	293
SalI	GTCGAC	1 cut(s)	1163
SaqAI	TTAA	2 cut(s)	578, 1798
SatI	GCNGC	3 cut(s)	646, 1278, 1355
Sau3AI	GATC	3 cut(s)	235, 249, 829
Sau96I	GGNCC	6 cut(s)	34, 35, 376, 433, 1486, 1598
SchI	GAGTC	8 cut(s)	95, 191, 605, 1026, 1322, 1556, 1727, 1774
SduI	GDGCHC	5 cut(s)	38, 293, 456, 543, 1771
SfaNI	GCATC	1 cut(s)	342
SfcI	CTRYAG	2 cut(s)	973, 1355
Sfr274I	CTCGAG	1 cut(s)	1253
SgrAI	CRCCGGYG	2 cut(s)	40, 935
SinI	GGWCC	4 cut(s)	376, 433, 1486, 1598
SlaI	CTCGAG	1 cut(s)	1253
SmaI	CCCGGG	1 cut(s)	1450
SmiMI	CAYNNNNRTG	4 cut(s)	635, 1079, 1696, 1774
SmlI	CTYRAG	4 cut(s)	286, 1040, 1253, 1604
SmoI	CTYRAG	4 cut(s)	286, 1040, 1253, 1604
SpeI	ACTAGT	1 cut(s)	317
Sse9I	AATT	8 cut(s)	347, 874, 1077, 1394, 1472, 1498, 1593, 1705
SspMI	CTAG	4 cut(s)	318, 839, 1520, 1662
SstI	GAGCTC	1 cut(s)	293
StyI	CCWWGG	3 cut(s)	60, 1539, 1694
TaaI	ACNGT	8 cut(s)	16, 168, 275, 380, 744, 951, 1227, 1408
TaiI	ACGT	6 cut(s)	259, 429, 511, 927, 1314, 1795
TaqI	TCGA	7 cut(s)	209, 438, 921, 1164, 1179, 1254, 1731
TaqII	GACCGA	1 cut(s)	167
TasI	AATT	8 cut(s)	347, 874, 1077, 1394, 1472, 1498, 1593, 1705
TatI	WGTACW	6 cut(s)	627, 951, 998, 1416, 1563, 1683
TauI	GCSGC	1 cut(s)	648
TfiI	GAWTC	2 cut(s)	130, 712
Tru1I	TTAA	2 cut(s)	578, 1798
Tru9I	TTAA	2 cut(s)	578, 1798
TscAI	CASTG	5 cut(s)	21, 417, 690, 1386, 1781
TseFI	GTSAC	4 cut(s)	16, 931, 1258, 1508
TseI	GCWGC	2 cut(s)	1277, 1354
Tsp45I	GTSAC	4 cut(s)	16, 931, 1258, 1508
TspDTI	ATGAA	6 cut(s)	64, 381, 431, 457, 838, 1096
TspGWI	ACGGA	4 cut(s)	113, 777, 948, 1355
TspMI	CCCGGG	1 cut(s)	1448
TspRI	CASTG	5 cut(s)	21, 417, 690, 1386, 1781
Tth111I	GACNNNGTC	2 cut(s)	808, 1227
VneI	GTGCAC	1 cut(s)	1767
VpaK11BI	GGWCC	4 cut(s)	376, 433, 1486, 1598
XagI	CCTNNNNNAGG	1 cut(s)	440
XapI	RAATTY	2 cut(s)	1394, 1705
XbaI	TCTAGA	2 cut(s)	1519, 1661
XhoI	CTCGAG	1 cut(s)	1253
XmaI	CCCGGG	1 cut(s)	1448
XmiI	GTMKAC	1 cut(s)	1164
XspI	CTAG	4 cut(s)	318, 839, 1520, 1662
ZraI	GACGTC	1 cut(s)	1793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.