Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015942

Physical Location & Seq

Genomic Coordinates Reverse (-)

19509787 .. 19511808

Gene Structure 2022 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1198700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGCCATCCCGTCCTCCACCATCTTCGCCACCCAACCACCACCACCACCGCCGTATTCTCTTACCACTGATAACCGTATCCATAACGCTAACTCTCGTCTCCCTCGTCGTCTTCCTTGTCTTCCTCTACCGCAAGCTGTCCTACAACCGCACAACCCCATTCGATCACCACCACAGCAGCGCCACCGCCACGCCCACCGCCACACCCAGCAAGAAGAGGGACAACAACCGTCGGTTCTCGTACGCTCTACTCCGGCGAGCCACCGGTTCGTTCTCGGCGTCGAACCGGCTGGGCCATGGCGGATTCGGCTCGGTCTACAAAGCCTCACTCCCCTCCGGCCAATCCCTCGCCGTGAAGCTCATGAACTCCAATGGCTCTCTCCAAGGTGAGCGGGAGTTTCACAATGAGCTCTCCATAGCTCTGAACCTCACCAATCCTCATATTCTCTCTCTTCTCGGCTTCTCCACTGACCGCCGCGGCCGGAAGCTGCTGCTGGTGTATGAGCTCATGCCTAATAGAAGCCTCCAGGAGGCGCTGTTGGATCGGAAGTGCCCGGAGCTTATGGTTTGGAAGAAGAGGTTCAGTGTTGTGCTTGATGTGGCTCGTGGGCTCGAGTATTTGCACCATGTTTGTGACCCGCCGGTCATTCATGGCGATGTCAAGCCTAGCAATGTGCTGCTGGACTCGGATTTCAATGCAAAGATTGGGGATTTTGGGTTGGCGAGGTTGAAAACAGAGGCGGAAGAGCAGGGGGAGCACGGCGGAGTTGTGGCGGTGGAGGAAGGGCAAGAGGAGGAGGAGAAAGAGAGCGGGGGAAGGGAGAAGAGGGATGGGGATGATAATGGGTCGATTTTGGAAGAGGAGAGTGTGAGTGTTAGTGTGAGTTTGAGTGTTGTGACTGGCGGGCTTGAAGAGGGCCGGTCGCCGGAGTGTTCTCTGGTGAGGATTGGGGATTCCGACGCTGCAGCGACGTCGCCGGAAGGGGGTTTTGATAAGGTGAGTGTTGAGAGTGAGATGGATTTGGGTTTGGGGAAGAGGGTTAAAGGAAGTTCAGGGAGGGACTGGTGGTGGAAACAGGACAATGTAGGCGGATTGGGGTCTGAGTCCGGGAGAGTCAAGGACTATGTGATGGAATGGATTGGGAGTGAAATAAAAAAGGAGAGGCCAAAGAGCGAATGGGTTGCGTCACCGAGCACAAGTGGTGTCAATGCTGAGCCAAAGAAGAAGGGATTCGATTGGTGGATGTCATCGGATGAGGAGAGGGGTAAGAAGAAGAAGGAGAAGAATCGGAAGCCGAGGGAGTGGTGGAAGGAGGAGTTTTGTGACGAGCTCACCAAGAAAAAGAAGAAGAAGAAGAGGGCTTTCGATGCGGCTAGTGGTGGTGGAGAGTTGTGGTGGCAAAAGGATGAGGATTTGGAGCAAGAGAAGAAGAAGAGGAAGAGCAAGAGCAAGAGCAGCAGAGGGAGTATTGATTGGTGGTTGGATGGAATGGGTAGTGATGTTAGAAGTGGGAGGCGGAATAGTCAAGATTGGGGCACTACTTGCGGTGTTGATATACCAAAAAGTGGTGGTATTAGTAGCACACCAAGCATGAGAGGCACAGTTTGTTATGTTGCCCCTGAATATGGCGGTGGTGGGCAGCTCTCCGAGAAATGTGATGTCTATAGCTTTGGAGTCTTGGTTTTGGTCTTGATTTCTGGTAGGAGGCCGCTGCAAGTCACGGCCTCCCCAATGTCAGAATTCGAAAGGGCCAATCTGATTTCGTGGGCTAGGCAGCTTGGTCGAAATGGGAAGCTTTTGGATCTCGTAGACCAATCTATACACTCTTTAGATAAGGAAGAGGCATTGCTATGCATCACAATTGCATTGCTATGTCTGCAGAGAACACCGGGCAAGCGGCCAACCATGAGGGAGATTGTGGGGATGCTTCTGGGCCAATCAGAGCCGCCGCATTTGCCCTTCGAATTTTCACCTTCGCCGCCTTCCAATTTTCCGTTCAAGTCCCGGAAAAAGGCCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

674

Amino Acids

74.82

Weight (kDa)

9.09

Isoelectric Point (pI)

55.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	93 - 247	1.2e-25	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	94 - 251	7.9e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	511 - 640	1.5e-08	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45590
fragaria_vesca	FvH4_7g12490
malus_domestica	MD02G1198700.v1.1
prunus_persica	Prupe.2G149500_v2.0.a1
pyrus_communis	pycom02g16260
rosa_chinensis	RchiOBHm_Chr1g0351331
rosa_laevigata	RLG00000028413
rosa_multiflora	Rmu_sc0004933.1_g000010
rosa_roxburghii	Rroxscaffold_4G00303870
rosa_rugosa	Rorug01G0217700
rosa_samantha	Rh1AG232200 Rh1BG202500 Rh1CG217000 Rh1DG229700
rosa_wichuraiana	Rw1G020220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	641
AatII	GACGTC	1 cut(s)	974
AccB7I	CCANNNNNTGG	1 cut(s)	1565
AccBSI	CCGCTC	2 cut(s)	391, 810
AccI	GTMKAC	2 cut(s)	315, 1809
AccII	CGCG	1 cut(s)	477
AclWI	GGATC	2 cut(s)	549, 1809
AcoI	YGGCCR	3 cut(s)	337, 478, 1898
AcsI	RAATTY	2 cut(s)	1739, 1964
AcyI	GRCGYC	2 cut(s)	278, 971
AfaI	GTAC	1 cut(s)	242
AfiI	CCNNNNNNNGG	6 cut(s)	529, 925, 983, 1565, 1625, 2011
AgeI	ACCGGT	1 cut(s)	263
AgsI	TTSAA	4 cut(s)	694, 730, 911, 1999
AjnI	CCWGG	1 cut(s)	525
AjuI	GAANNNNNNNTTGG	2 cut(s)	1979, 2011
Alw21I	GWGCWC	5 cut(s)	411, 507, 759, 1196, 1332
Alw26I	GTCTC	1 cut(s)	103
AlwI	GGATC	2 cut(s)	549, 1809
Ama87I	CYCGRG	1 cut(s)	611
ApoI	RAATTY	2 cut(s)	1739, 1964
AsiGI	ACCGGT	1 cut(s)	263
Asp700I	GAANNNNTTC	2 cut(s)	578, 1229
AspLEI	GCGC	2 cut(s)	182, 535
AspS9I	GGNCC	5 cut(s)	292, 916, 1749, 1933, 2014
AsuC2I	CCSGG	5 cut(s)	554, 1108, 1890, 2005, 2017
AsuHPI	GGTGA	8 cut(s)	158, 398, 421, 952, 1009, 1179, 1324, 1962
AsuII	TTCGAA	2 cut(s)	1743, 1962
AvaI	CYCGRG	1 cut(s)	611
BaeGI	GKGCMC	2 cut(s)	554, 1538
BanII	GRGCYC	4 cut(s)	411, 507, 612, 1332
BauI	CACGAG	1 cut(s)	603
BbsI	GAAGAC	2 cut(s)	103, 112
Bbv12I	GWGCWC	5 cut(s)	411, 507, 759, 1196, 1332
BccI	CCATC	6 cut(s)	13, 28, 824, 1009, 1123, 1478
BceAI	ACGGC	4 cut(s)	36, 335, 775, 1737
BcgI	CGANNNNNNTGC	6 cut(s)	954, 988, 1163, 1197, 1763, 1797
BciT130I	CCWGG	1 cut(s)	527
BciVI	GTATCC	1 cut(s)	88
BcnI	CCSGG	5 cut(s)	554, 1108, 1890, 2005, 2017
BcoDI	GTCTC	1 cut(s)	103
BfaI	CTAG	3 cut(s)	666, 1374, 1770
BfmI	CTRYAG	3 cut(s)	963, 1663, 1877
BfoI	RGCGCY	2 cut(s)	183, 536
BfuI	GTATCC	1 cut(s)	88
BlpI	GCTNAGC	1 cut(s)	1212
Bme1390I	CCNGG	6 cut(s)	527, 554, 1108, 1890, 2005, 2017
BmeT110I	CYCGRG	1 cut(s)	611
BmgT120I	GGNCC	5 cut(s)	292, 916, 1749, 1933, 2014
BmrFI	CCNGG	6 cut(s)	527, 554, 1108, 1890, 2005, 2017
BmsI	GCATC	3 cut(s)	1357, 1863, 1914
BpiI	GAAGAC	2 cut(s)	103, 112
BpmI	CTGGAG	1 cut(s)	509
Bpu1102I	GCTNAGC	1 cut(s)	1212
Bpu14I	TTCGAA	2 cut(s)	1743, 1962
BpuMI	CCSGG	5 cut(s)	554, 1108, 1890, 2005, 2017
BsaHI	GRCGYC	2 cut(s)	278, 971
BsaJI	CCNNGG	4 cut(s)	295, 382, 475, 1295
BsaWI	WCCGGW	1 cut(s)	263
BsaXI	ACNNNNNCTCC	4 cut(s)	521, 551, 1665, 1695
Bsc4I	CCNNNNNNNGG	6 cut(s)	529, 925, 983, 1565, 1625, 2011
Bse118I	RCCGGY	4 cut(s)	263, 285, 640, 918
Bse1I	ACTGG	2 cut(s)	904, 1067
Bse3DI	GCAATG	3 cut(s)	676, 1844, 1865
BseBI	CCWGG	1 cut(s)	527
BseDI	CCNNGG	4 cut(s)	295, 382, 475, 1295
BseGI	GGATG	8 cut(s)	5, 835, 841, 1248, 1258, 1411, 1489, 1929
BseLI	CCNNNNNNNGG	6 cut(s)	529, 925, 983, 1565, 1625, 2011
BseMI	GCAATG	3 cut(s)	676, 1844, 1865
BseMII	CTCAG	2 cut(s)	1092, 1203
BseNI	ACTGG	2 cut(s)	904, 1067
BseRI	GAGGAG	6 cut(s)	806, 809, 812, 875, 1271, 1328
BseSI	GKGCMC	2 cut(s)	554, 1538
BseX3I	CGGCCG	1 cut(s)	478
BseYI	CCCAGC	2 cut(s)	206, 289
Bsh1236I	CGCG	1 cut(s)	477
Bsh1285I	CGRYCG	2 cut(s)	481, 923
BshTI	ACCGGT	1 cut(s)	263
BsiEI	CGRYCG	2 cut(s)	481, 923
BsiHKAI	GWGCWC	5 cut(s)	411, 507, 759, 1196, 1332
BsiHKCI	CYCGRG	1 cut(s)	611
BsiWI	CGTACG	1 cut(s)	240
BslFI	GGGAC	3 cut(s)	233, 1073, 1987
BslI	CCNNNNNNNGG	6 cut(s)	529, 925, 983, 1565, 1625, 2011
BsmAI	GTCTC	1 cut(s)	103
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	3 cut(s)	233, 1073, 1987
BsoBI	CYCGRG	1 cut(s)	611
Bsp119I	TTCGAA	2 cut(s)	1743, 1962
Bsp1286I	GDGCHC	8 cut(s)	411, 507, 554, 612, 759, 1196, 1332, 1538
Bsp143I	GATC	3 cut(s)	163, 541, 1801
Bsp1720I	GCTNAGC	1 cut(s)	1212
Bsp19I	CCATGG	1 cut(s)	295
BspCNI	CTCAG	2 cut(s)	1093, 1204
BspFNI	CGCG	1 cut(s)	477
BspHI	TCATGA	1 cut(s)	360
BspMAI	CTGCAG	2 cut(s)	967, 1881
BspPI	GGATC	2 cut(s)	549, 1809
BspQI	GCTCTTC	2 cut(s)	738, 1433
BspT104I	TTCGAA	2 cut(s)	1743, 1962
BsrBI	CCGCTC	2 cut(s)	391, 810
BsrDI	GCAATG	3 cut(s)	676, 1844, 1865
BsrFI	RCCGGY	4 cut(s)	263, 285, 640, 918
BsrI	ACTGG	2 cut(s)	904, 1067
BssAI	RCCGGY	4 cut(s)	263, 285, 640, 918
BssECI	CCNNGG	4 cut(s)	295, 382, 475, 1295
BssMI	GATC	3 cut(s)	163, 541, 1801
BssNI	GRCGYC	2 cut(s)	278, 971
BssSI	CACGAG	1 cut(s)	603
BssT1I	CCWWGG	2 cut(s)	295, 382
Bst2BI	CACGAG	1 cut(s)	603
Bst2UI	CCWGG	1 cut(s)	527
Bst4CI	ACNGT	3 cut(s)	76, 230, 1603
BstACI	GRCGYC	2 cut(s)	278, 971
BstAPI	GCANNNNNTGC	1 cut(s)	1542
BstBI	TTCGAA	2 cut(s)	1743, 1962
BstC8I	GCNNGC	4 cut(s)	134, 258, 905, 1895
BstDEI	CTNAG	2 cut(s)	1101, 1212
BstDSI	CCRYGG	2 cut(s)	295, 475
BstENI	CCTNNNNNAGG	1 cut(s)	527
BstF5I	GGATG	8 cut(s)	5, 835, 841, 1248, 1258, 1411, 1489, 1929
BstFNI	CGCG	1 cut(s)	477
BstH2I	RGCGCY	2 cut(s)	183, 536
BstHHI	GCGC	2 cut(s)	182, 535
BstKTI	GATC	3 cut(s)	166, 544, 1804
BstMAI	GTCTC	1 cut(s)	103
BstMBI	GATC	3 cut(s)	163, 541, 1801
BstMCI	CGRYCG	2 cut(s)	481, 923
BstMWI	GCNNNNNNNGC	9 cut(s)	306, 754, 1367, 1455, 1542, 1587, 1596, 1876, 1954
BstNI	CCWGG	1 cut(s)	527
BstSCI	CCNGG	6 cut(s)	525, 552, 1106, 1888, 2003, 2015
BstSFI	CTRYAG	3 cut(s)	963, 1663, 1877
BstSLI	GKGCMC	2 cut(s)	554, 1538
BstUI	CGCG	1 cut(s)	477
BstV2I	GAAGAC	2 cut(s)	103, 112
BstX2I	RGATCY	1 cut(s)	1801
BstYI	RGATCY	1 cut(s)	1801
BstZI	CGGCCG	1 cut(s)	478
BsuI	GTATCC	1 cut(s)	88
BtgI	CCRYGG	2 cut(s)	295, 475
BtgZI	GCGATG	1 cut(s)	669
BtsCI	GGATG	8 cut(s)	5, 835, 841, 1248, 1258, 1411, 1489, 1929
BtsIMutI	CAGTG	3 cut(s)	65, 465, 589
Cac8I	GCNNGC	4 cut(s)	134, 258, 905, 1895
CciI	TCATGA	1 cut(s)	360
CfoI	GCGC	2 cut(s)	182, 535
Cfr10I	RCCGGY	4 cut(s)	263, 285, 640, 918
Cfr13I	GGNCC	5 cut(s)	292, 916, 1749, 1933, 2014
Cfr42I	CCGCGG	1 cut(s)	478
CseI	GACGC	3 cut(s)	267, 968, 1173
Csp6I	GTAC	1 cut(s)	241
CspAI	ACCGGT	1 cut(s)	263
CviAII	CATG	7 cut(s)	296, 361, 508, 626, 650, 1591, 1906
CviQI	GTAC	1 cut(s)	241
DdeI	CTNAG	2 cut(s)	1101, 1212
DpnI	GATC	3 cut(s)	165, 543, 1803
DpnII	GATC	3 cut(s)	163, 541, 1801
DrdI	GACNNNNNNGTC	1 cut(s)	641
DseDI	GACNNNNNNGTC	1 cut(s)	641
EaeI	YGGCCR	3 cut(s)	337, 478, 1898
EagI	CGGCCG	1 cut(s)	478
EciI	GGCGGA	5 cut(s)	315, 755, 777, 1104, 1531
Ecl136II	GAGCTC	3 cut(s)	409, 505, 1330
EclXI	CGGCCG	1 cut(s)	478
Eco130I	CCWWGG	2 cut(s)	295, 382
Eco24I	GRGCYC	4 cut(s)	411, 507, 612, 1332
Eco52I	CGGCCG	1 cut(s)	478
Eco53kI	GAGCTC	3 cut(s)	409, 505, 1330
Eco88I	CYCGRG	1 cut(s)	611
EcoICRI	GAGCTC	3 cut(s)	409, 505, 1330
EcoNI	CCTNNNNNAGG	1 cut(s)	527
EcoRI	GAATTC	1 cut(s)	1739
EcoRII	CCWGG	1 cut(s)	525
EcoT14I	CCWWGG	2 cut(s)	295, 382
EcoT22I	ATGCAT	1 cut(s)	1856
EcoT38I	GRGCYC	4 cut(s)	411, 507, 612, 1332
ErhI	CCWWGG	2 cut(s)	295, 382
Esp3I	CGTCTC	1 cut(s)	103
FaeI	CATG	7 cut(s)	299, 364, 511, 629, 653, 1594, 1909
FaqI	GGGAC	3 cut(s)	233, 1073, 1987
FatI	CATG	7 cut(s)	295, 360, 507, 625, 649, 1590, 1905
FauI	CCCGC	4 cut(s)	384, 645, 803, 896
FblI	GTMKAC	2 cut(s)	315, 1809
FokI	GGATG	7 cut(s)	842, 848, 1255, 1265, 1418, 1496, 1936
FriOI	GRGCYC	4 cut(s)	411, 507, 612, 1332
FspBI	CTAG	3 cut(s)	666, 1374, 1770
GlaI	GCGC	2 cut(s)	181, 534
GsaI	CCCAGC	2 cut(s)	210, 293
GsuI	CTGGAG	1 cut(s)	509
HaeII	RGCGCY	2 cut(s)	183, 536
HgaI	GACGC	3 cut(s)	267, 968, 1173
HhaI	GCGC	2 cut(s)	182, 535
Hin1I	GRCGYC	2 cut(s)	278, 971
Hin1II	CATG	7 cut(s)	299, 364, 511, 629, 653, 1594, 1909
Hin6I	GCGC	2 cut(s)	180, 533
HinP1I	GCGC	2 cut(s)	180, 533
HindIII	AAGCTT	1 cut(s)	1793
HinfI	GANTC	8 cut(s)	303, 683, 953, 1103, 1113, 1230, 1285, 1674
HphI	GGTGA	8 cut(s)	158, 398, 421, 952, 1009, 1179, 1324, 1962
Hpy166II	GTNNAC	2 cut(s)	316, 1810
Hpy188III	TCNNGA	3 cut(s)	361, 1526, 1690
Hpy8I	GTNNAC	2 cut(s)	316, 1810
Hpy99I	CGWCG	6 cut(s)	110, 234, 283, 962, 973, 976
HpyAV	CCTTC	9 cut(s)	776, 810, 974, 1219, 1270, 1303, 1969, 1983, 1992
HpyCH4III	ACNGT	3 cut(s)	76, 230, 1603
HpyCH4IV	ACGT	1 cut(s)	971
HpyCH4V	TGCA	7 cut(s)	622, 698, 965, 1714, 1854, 1865, 1879
HpyF10VI	GCNNNNNNNGC	9 cut(s)	306, 754, 1367, 1455, 1542, 1587, 1596, 1876, 1954
HpyF3I	CTNAG	2 cut(s)	1101, 1212
HpySE526I	ACGT	1 cut(s)	971
Hsp92I	GRCGYC	2 cut(s)	278, 971
Hsp92II	CATG	7 cut(s)	299, 364, 511, 629, 653, 1594, 1909
HspAI	GCGC	2 cut(s)	180, 533
KspI	CCGCGG	1 cut(s)	478
Kzo9I	GATC	3 cut(s)	163, 541, 1801
LguI	GCTCTTC	2 cut(s)	738, 1433
LmnI	GCTCC	3 cut(s)	556, 754, 1417
LweI	GCATC	3 cut(s)	1357, 1863, 1914
MaeI	CTAG	3 cut(s)	666, 1374, 1770
MaeII	ACGT	1 cut(s)	971
MaeIII	GTNAC	5 cut(s)	632, 895, 1185, 1322, 1717
MalI	GATC	3 cut(s)	165, 543, 1803
MbiI	CCGCTC	2 cut(s)	391, 810
MboI	GATC	3 cut(s)	163, 541, 1801
MfeI	CAATTG	1 cut(s)	1860
MflI	RGATCY	1 cut(s)	1801
MhlI	GDGCHC	8 cut(s)	411, 507, 554, 612, 759, 1196, 1332, 1538
MluCI	AATT	4 cut(s)	1739, 1860, 1964, 1987
MlyI	GAGTC	4 cut(s)	677, 1112, 1122, 1683
MmeI	TCCRAC	3 cut(s)	519, 981, 1461
Mph1103I	ATGCAT	1 cut(s)	1856
MroXI	GAANNNNTTC	2 cut(s)	578, 1229
MseI	TTAA	1 cut(s)	1041
MslI	CAYNNNNRTG	4 cut(s)	630, 654, 1849, 1870
MspA1I	CMGCKG	2 cut(s)	477, 1711
MspR9I	CCNGG	6 cut(s)	527, 554, 1108, 1890, 2005, 2017
MunI	CAATTG	1 cut(s)	1860
MvaI	CCWGG	1 cut(s)	527
MvnI	CGCG	1 cut(s)	477
MwoI	GCNNNNNNNGC	9 cut(s)	306, 754, 1367, 1455, 1542, 1587, 1596, 1876, 1954
NciI	CCSGG	5 cut(s)	554, 1108, 1890, 2005, 2017
NcoI	CCATGG	1 cut(s)	295
NdeII	GATC	3 cut(s)	163, 541, 1801
NlaIII	CATG	7 cut(s)	299, 364, 511, 629, 653, 1594, 1909
NmeAIII	GCCGAG	3 cut(s)	254, 435, 1320
NmuCI	GTSAC	5 cut(s)	632, 895, 1185, 1322, 1717
NsiI	ATGCAT	1 cut(s)	1856
NspV	TTCGAA	2 cut(s)	1743, 1962
PaeR7I	CTCGAG	1 cut(s)	611
PagI	TCATGA	1 cut(s)	360
PciSI	GCTCTTC	2 cut(s)	738, 1433
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 275
PdmI	GAANNNNTTC	2 cut(s)	578, 1229
PfeI	GAWTC	4 cut(s)	303, 953, 1230, 1285
Pfl23II	CGTACG	1 cut(s)	240
PflMI	CCANNNNNTGG	1 cut(s)	1565
PfoI	TCCNGGA	3 cut(s)	525, 1106, 2003
PinAI	ACCGGT	1 cut(s)	263
PleI	GAGTC	4 cut(s)	677, 1111, 1121, 1682
PpsI	GAGTC	4 cut(s)	677, 1111, 1121, 1682
Psp124BI	GAGCTC	3 cut(s)	411, 507, 1332
Psp6I	CCWGG	1 cut(s)	525
PspFI	CCCAGC	2 cut(s)	206, 289
PspGI	CCWGG	1 cut(s)	525
PspLI	CGTACG	1 cut(s)	240
PspPI	GGNCC	5 cut(s)	292, 916, 1749, 1933, 2014
PspXI	VCTCGAGB	1 cut(s)	611
PstI	CTGCAG	2 cut(s)	967, 1881
PsuI	RGATCY	1 cut(s)	1801
RsaI	GTAC	1 cut(s)	242
RsaNI	GTAC	1 cut(s)	241
RseI	CAYNNNNRTG	4 cut(s)	630, 654, 1849, 1870
SacI	GAGCTC	3 cut(s)	411, 507, 1332
SacII	CCGCGG	1 cut(s)	478
SapI	GCTCTTC	2 cut(s)	738, 1433
SaqAI	TTAA	1 cut(s)	1041
Sau3AI	GATC	3 cut(s)	163, 541, 1801
Sau96I	GGNCC	5 cut(s)	292, 916, 1749, 1933, 2014
SchI	GAGTC	4 cut(s)	677, 1112, 1122, 1683
ScrFI	CCNGG	6 cut(s)	527, 554, 1108, 1890, 2005, 2017
SduI	GDGCHC	8 cut(s)	411, 507, 554, 612, 759, 1196, 1332, 1538
SfaNI	GCATC	3 cut(s)	1357, 1863, 1914
SfcI	CTRYAG	3 cut(s)	963, 1663, 1877
Sfr274I	CTCGAG	1 cut(s)	611
Sfr303I	CCGCGG	1 cut(s)	478
SfuI	TTCGAA	2 cut(s)	1743, 1962
SgrBI	CCGCGG	1 cut(s)	478
SlaI	CTCGAG	1 cut(s)	611
SmiMI	CAYNNNNRTG	4 cut(s)	630, 654, 1849, 1870
SmlI	CTYRAG	1 cut(s)	611
SmoI	CTYRAG	1 cut(s)	611
Sse9I	AATT	4 cut(s)	1739, 1860, 1964, 1987
SspMI	CTAG	3 cut(s)	666, 1374, 1770
SstI	GAGCTC	3 cut(s)	411, 507, 1332
StyD4I	CCNGG	6 cut(s)	525, 552, 1106, 1888, 2003, 2015
StyI	CCWWGG	2 cut(s)	295, 382
TaaI	ACNGT	3 cut(s)	76, 230, 1603
TaiI	ACGT	1 cut(s)	974
TaqI	TCGA	9 cut(s)	162, 281, 612, 848, 1233, 1365, 1743, 1783, 1962
TaqII	GACCGA	1 cut(s)	301
TasI	AATT	4 cut(s)	1739, 1860, 1964, 1987
TauI	GCSGC	8 cut(s)	477, 480, 1373, 1711, 1900, 1948, 1951, 1981
TfiI	GAWTC	4 cut(s)	303, 953, 1230, 1285
Tru1I	TTAA	1 cut(s)	1041
Tru9I	TTAA	1 cut(s)	1041
TscAI	CASTG	3 cut(s)	72, 472, 589
TseFI	GTSAC	5 cut(s)	632, 895, 1185, 1322, 1717
Tsp45I	GTSAC	5 cut(s)	632, 895, 1185, 1322, 1717
TspDTI	ATGAA	2 cut(s)	377, 638
TspGWI	ACGGA	1 cut(s)	1983
TspRI	CASTG	3 cut(s)	72, 472, 589
Van91I	CCANNNNNTGG	1 cut(s)	1565
XagI	CCTNNNNNAGG	1 cut(s)	527
XapI	RAATTY	2 cut(s)	1739, 1964
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1173
XhoI	CTCGAG	1 cut(s)	611
XmiI	GTMKAC	2 cut(s)	315, 1809
XmnI	GAANNNNTTC	2 cut(s)	578, 1229
XspI	CTAG	3 cut(s)	666, 1374, 1770
ZraI	GACGTC	1 cut(s)	972
Zsp2I	ATGCAT	1 cut(s)	1856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.