Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0022267

Physical Location & Seq

Genomic Coordinates Reverse (-)

33724739 .. 33726075

Gene Structure 1337 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1281400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 522 bp

ATGGCTCCTAAAAACCTTCTACGGCACAAGGACTGGAAATCAAATTTATCCGAGTTCGATGATGTGCAAGGCCACCCAGGCTTTGACAAGCAGGGGACCAGATTTAAGCACCTCATCAAGGACCAGAATCACCACTCTGATCTTGAGGAGGGTATTAGACGGGTGGTTCTGTGCTCCGGCAAGCTTTATTATGAACTTGATGGAGAGCGAAGAAAGGTTGGAGCAAAGGATGTTGCAATTCGTAGGGTGGAACAGCTTTGCCCCTTCCCTTACGACCTCATCCAGCAAGAGCTGAAGCGATATCCAAATGCTGAGATAGTTTGGTGTCAGGAAGAGCCGATGAACATGGGTGCGTGCAGCTATGTTGCACCCCGCCTTAGTGTTGCCATGAAGTCCCTGGGCAGAGGAACTATGGACGGTGTCAAATACATTGGCCGTGATCCATCAGCTGCCACAGCCACTGGTTTTTCTCAGGTTCATGATATGGAACAAAACGAGATTTTGCAGAAAGCGATGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004591 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0009060 GO:0009353 GO:0009987 GO:0015980 GO:0016491 GO:0016624 GO:0016903 GO:0016999 GO:0017144 GO:0019752 GO:0030062 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0045252 GO:0045333 GO:0055114 GO:0070013 GO:0071704 GO:0072350 GO:0098798 GO:1902494 GO:1990204 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00020 ko00310 ko00380 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map00380 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxogl_dehyd_N E1_dh OxoGdeHyase_C Transket_pyr

Protein Analysis

174

Amino Acids

19.78

Weight (kDa)

7.7

Isoelectric Point (pI)

46.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OxoGdeHyase_C	PF16870	31 - 172	6.9e-50	2-oxoglutarate dehydrogenase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0022267)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g21780
malus_domestica	MD02G1281400.v1.1
pyrus_communis	pycom02g24060
rosa_samantha	Rh1CG312500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	373
AclWI	GGATC	1 cut(s)	434
AcoI	YGGCCR	1 cut(s)	433
AcsI	RAATTY	1 cut(s)	43
AcuI	CTGAAG	1 cut(s)	314
AfiI	CCNNNNNNNGG	1 cut(s)	118
AjnI	CCWGG	2 cut(s)	76, 396
AluBI	AGCT	5 cut(s)	184, 256, 292, 360, 449
AluI	AGCT	5 cut(s)	184, 256, 292, 360, 449
Alw21I	GWGCWC	1 cut(s)	176
AlwI	GGATC	1 cut(s)	434
AlwNI	CAGNNNCTG	1 cut(s)	461
AoxI	GGCC	2 cut(s)	70, 433
ApeKI	GCWGC	2 cut(s)	357, 449
ApoI	RAATTY	1 cut(s)	43
AspS9I	GGNCC	2 cut(s)	96, 121
AsuHPI	GGTGA	1 cut(s)	122
AvaII	GGWCC	2 cut(s)	96, 121
Bbv12I	GWGCWC	1 cut(s)	176
BbvI	GCAGC	2 cut(s)	369, 436
BccI	CCATC	2 cut(s)	194, 451
BceAI	ACGGC	2 cut(s)	38, 420
BciT130I	CCWGG	2 cut(s)	78, 398
BglI	GCCNNNNNGGC	1 cut(s)	78
BisI	GCNGC	2 cut(s)	358, 450
BlsI	GCNGC	2 cut(s)	359, 451
Bme1390I	CCNGG	2 cut(s)	78, 398
Bme18I	GGWCC	2 cut(s)	96, 121
BmgT120I	GGNCC	2 cut(s)	96, 121
BmiI	GGNNCC	2 cut(s)	6, 97
BmrFI	CCNGG	2 cut(s)	78, 398
BmsI	GCATC	1 cut(s)	504
BpuEI	CTTGAG	1 cut(s)	164
BsaJI	CCNNGG	3 cut(s)	76, 396, 397
Bsc4I	CCNNNNNNNGG	1 cut(s)	118
Bse1I	ACTGG	2 cut(s)	38, 466
BseBI	CCWGG	2 cut(s)	78, 398
BseDI	CCNNGG	3 cut(s)	76, 396, 397
BseGI	GGATG	2 cut(s)	235, 279
BseLI	CCNNNNNNNGG	1 cut(s)	118
BseMII	CTCAG	2 cut(s)	303, 485
BseNI	ACTGG	2 cut(s)	38, 466
BseRI	GAGGAG	1 cut(s)	161
BseXI	GCAGC	2 cut(s)	369, 436
BsgI	GTGCAG	1 cut(s)	376
BshFI	GGCC	2 cut(s)	72, 435
BsiHKAI	GWGCWC	1 cut(s)	176
BsiSI	CCGG	1 cut(s)	177
BslFI	GGGAC	2 cut(s)	109, 379
BslI	CCNNNNNNNGG	1 cut(s)	118
BsmFI	GGGAC	2 cut(s)	109, 379
BsnI	GGCC	2 cut(s)	72, 435
Bsp1286I	GDGCHC	1 cut(s)	176
Bsp143I	GATC	2 cut(s)	139, 439
BspACI	CCGC	1 cut(s)	373
BspANI	GGCC	2 cut(s)	72, 435
BspCNI	CTCAG	2 cut(s)	304, 484
BspHI	TCATGA	1 cut(s)	478
BspLI	GGNNCC	2 cut(s)	6, 97
BspPI	GGATC	1 cut(s)	434
BspQI	GCTCTTC	1 cut(s)	327
BsrI	ACTGG	2 cut(s)	38, 466
BssECI	CCNNGG	3 cut(s)	76, 396, 397
BssMI	GATC	2 cut(s)	139, 439
Bst2UI	CCWGG	2 cut(s)	78, 398
Bst4CI	ACNGT	1 cut(s)	419
Bst6I	CTCTTC	1 cut(s)	327
BstC8I	GCNNGC	2 cut(s)	182, 355
BstDEI	CTNAG	3 cut(s)	312, 377, 471
BstENI	CCTNNNNNAGG	1 cut(s)	116
BstF5I	GGATG	2 cut(s)	235, 279
BstKTI	GATC	2 cut(s)	142, 442
BstMBI	GATC	2 cut(s)	139, 439
BstMWI	GCNNNNNNNGC	2 cut(s)	78, 455
BstNI	CCWGG	2 cut(s)	78, 398
BstSCI	CCNGG	2 cut(s)	76, 396
BstV1I	GCAGC	2 cut(s)	369, 436
BsuRI	GGCC	2 cut(s)	72, 435
BtsCI	GGATG	2 cut(s)	235, 279
BtsIMutI	CAGTG	1 cut(s)	459
Cac8I	GCNNGC	2 cut(s)	182, 355
CaiI	CAGNNNCTG	1 cut(s)	461
CciI	TCATGA	1 cut(s)	478
Cfr13I	GGNCC	2 cut(s)	96, 121
CviAII	CATG	3 cut(s)	346, 388, 479
DdeI	CTNAG	3 cut(s)	312, 377, 471
DpnI	GATC	2 cut(s)	141, 441
DpnII	GATC	2 cut(s)	139, 439
EaeI	YGGCCR	1 cut(s)	433
Eam1104I	CTCTTC	1 cut(s)	327
EarI	CTCTTC	1 cut(s)	327
Eco32I	GATATC	1 cut(s)	302
Eco47I	GGWCC	2 cut(s)	96, 121
Eco57I	CTGAAG	1 cut(s)	314
EcoNI	CCTNNNNNAGG	1 cut(s)	116
EcoRII	CCWGG	2 cut(s)	76, 396
EcoRV	GATATC	1 cut(s)	302
FaeI	CATG	3 cut(s)	349, 391, 482
FaiI	YATR	7 cut(s)	192, 347, 363, 389, 413, 480, 485
FaqI	GGGAC	2 cut(s)	109, 379
FatI	CATG	3 cut(s)	345, 387, 478
FauI	CCCGC	1 cut(s)	380
Fnu4HI	GCNGC	2 cut(s)	358, 450
FokI	GGATG	2 cut(s)	242, 266
Fsp4HI	GCNGC	2 cut(s)	358, 450
GluI	GCNGC	2 cut(s)	358, 450
HaeIII	GGCC	2 cut(s)	72, 435
HapII	CCGG	1 cut(s)	177
Hin1II	CATG	3 cut(s)	349, 391, 482
HindIII	AAGCTT	1 cut(s)	182
HinfI	GANTC	1 cut(s)	127
HpaII	CCGG	1 cut(s)	177
HphI	GGTGA	1 cut(s)	122
Hpy188I	TCNGA	2 cut(s)	52, 139
Hpy188III	TCNNGA	3 cut(s)	143, 329, 479
HpyAV	CCTTC	2 cut(s)	26, 274
HpyCH4III	ACNGT	1 cut(s)	419
HpyCH4V	TGCA	6 cut(s)	67, 236, 357, 368, 505, 517
HpyF10VI	GCNNNNNNNGC	2 cut(s)	78, 455
HpyF3I	CTNAG	3 cut(s)	312, 377, 471
Hsp92II	CATG	3 cut(s)	349, 391, 482
Kzo9I	GATC	2 cut(s)	139, 439
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	3 cut(s)	10, 179, 221
Lsp1109I	GCAGC	2 cut(s)	369, 436
LweI	GCATC	1 cut(s)	504
MalI	GATC	2 cut(s)	141, 441
MboI	GATC	2 cut(s)	139, 439
MboII	GAAGA	2 cut(s)	222, 344
MhlI	GDGCHC	1 cut(s)	176
MluCI	AATT	2 cut(s)	43, 237
MmeI	TCCRAC	1 cut(s)	199
MnlI	CCTC	5 cut(s)	122, 139, 142, 287, 398
MseI	TTAA	1 cut(s)	105
MspA1I	CMGCKG	1 cut(s)	449
MspI	CCGG	1 cut(s)	177
MspR9I	CCNGG	2 cut(s)	78, 398
MvaI	CCWGG	2 cut(s)	78, 398
MwoI	GCNNNNNNNGC	2 cut(s)	78, 455
NdeII	GATC	2 cut(s)	139, 439
NlaIII	CATG	3 cut(s)	349, 391, 482
NlaIV	GGNNCC	2 cut(s)	6, 97
PagI	TCATGA	1 cut(s)	478
PasI	CCCWGGG	1 cut(s)	397
PciSI	GCTCTTC	1 cut(s)	327
PfeI	GAWTC	1 cut(s)	127
PflFI	GACNNNGTC	1 cut(s)	419
PkrI	GCNGC	2 cut(s)	359, 451
Psp6I	CCWGG	2 cut(s)	76, 396
PspGI	CCWGG	2 cut(s)	76, 396
PspN4I	GGNNCC	2 cut(s)	6, 97
PspPI	GGNCC	2 cut(s)	96, 121
PstNI	CAGNNNCTG	1 cut(s)	461
PsyI	GACNNNGTC	1 cut(s)	419
PvuII	CAGCTG	1 cut(s)	449
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	1 cut(s)	105
SatI	GCNGC	2 cut(s)	358, 450
Sau3AI	GATC	2 cut(s)	139, 439
Sau96I	GGNCC	2 cut(s)	96, 121
ScrFI	CCNGG	2 cut(s)	78, 398
SduI	GDGCHC	1 cut(s)	176
SfaNI	GCATC	1 cut(s)	504
SinI	GGWCC	2 cut(s)	96, 121
SmlI	CTYRAG	1 cut(s)	143
SmoI	CTYRAG	1 cut(s)	143
Sse9I	AATT	2 cut(s)	43, 237
SsiI	CCGC	1 cut(s)	373
StyD4I	CCNGG	2 cut(s)	76, 396
TaaI	ACNGT	1 cut(s)	419
TaqI	TCGA	1 cut(s)	57
TasI	AATT	2 cut(s)	43, 237
TfiI	GAWTC	1 cut(s)	127
Tru1I	TTAA	1 cut(s)	105
Tru9I	TTAA	1 cut(s)	105
TscAI	CASTG	1 cut(s)	466
TseI	GCWGC	2 cut(s)	357, 449
TspDTI	ATGAA	4 cut(s)	207, 356, 404, 467
TspRI	CASTG	1 cut(s)	466
Tth111I	GACNNNGTC	1 cut(s)	419
VpaK11BI	GGWCC	2 cut(s)	96, 121
XagI	CCTNNNNNAGG	1 cut(s)	116
XapI	RAATTY	1 cut(s)	43
XcmI	CCANNNNNNNNNTGG	1 cut(s)	394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.