MD02G1295100.v1.1

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0022377
Physical Location & Seq
Forward (+)
34969355 .. 34972337
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1295100.v1.1.491

Sequence Viewer

Length: 843 bp
ATGAAGACCCACAAAGCTTCGAATTCGAACAAGTCCTCTAATGGAAGGAGGATCGAAAAGAGGGGAATGAAGAGAAAGGCCTTGGAGCGATGCTCAAGGCTTAAGTCTTCCAAGGACTTCTTGGAAGTCAAAGGAATTATTGAACAGGATAGTAATGAAGATTCTATGCTCCAACTTCCCGGCATTCCTCTAGCTCCTTCCAGCCAGAATATGAACCCTGGAGTTACTTTTGTTCTCGAAAAGGCCTCCTTGGTGCCTGCTTATGTCGGCAAGAATTACCAGATTTTAAATCCAGAAATACACGGGGACTTCCTTCGGAAAAAGAATATGGATCCTTATAAATACAGACCTGACATTGTTCACGAGGCCCTCCTTCAAATTATGGATACCCGACTTTGCATGGCCGGCAGACTGCAGCCGGTGTATGTTAAAACTGACCAAGGGGTTCTCATCAAAGTCGAACCACATGCTCGTATACCAGAATCATTGGACAAGTTTTGTGCTATGATGTCACAATTATTGCAAAAGCTCCGCATTAAAGCCAAGGGTCGACGTGATACACTCTTGCGTGTGGTTCAGAACCCTTTATCTCAGCATTTACCTTCTAACTCCGTCAAAATAGGCCTTTCCGTCAGTTCGCAAAAGGAAGTTCAGTTACGGGAGTATGTAAATGCTGTCAGCAATAATGCCAACCTTGTATTTGTGGTTGGTGCCATGGCTCACGGGAAAATTGATAGCGAGTATGCGGATGATTTCATATCAGTTTCTGGATATCCGCTGAGCGCTGGGGTGTGCCTGAGAAGTATATGTATAGCATTGGAGAGAAAGTGGAATATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

281

Amino Acids

31.42

Weight (kDa)

9.65

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMG1 PF03587 77 - 274 1.5e-55 EMG1/NEP1 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022377)

Species Orthologous Gene IDs
malus_domestica MD02G1295100.v1.1
prunus_persica Prupe.1G567900_v2.0.a1 Prupe.1G569000_v2.0.a1
pyrus_communis pycom02g24810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB1I GGYRCC 2 cut(s) 253, 710
AccI GTMKAC 2 cut(s) 475, 550
AciI CCGC 3 cut(s) 532, 746, 776
AclWI GGATC 3 cut(s) 59, 326, 339
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 1 cut(s) 22
AfeI AGCGCT 1 cut(s) 784
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 2 cut(s) 143, 377
AjiI CACGTC 1 cut(s) 554
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 3 cut(s) 17, 194, 529
AluI AGCT 3 cut(s) 17, 194, 529
AlwI GGATC 3 cut(s) 59, 326, 339
AlwNI CAGNNNCTG 1 cut(s) 767
Aor51HI AGCGCT 1 cut(s) 784
AoxI GGCC 5 cut(s) 78, 243, 366, 402, 622
ApeKI GCWGC 1 cut(s) 415
ApoI RAATTY 1 cut(s) 22
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 180
AsuII TTCGAA 2 cut(s) 20, 26
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 2 cut(s) 253, 710
BarI GAAGNNNNNNTAC 2 cut(s) 639, 671
BauI CACGAG 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 11, 99
BbvI GCAGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 219
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 786
BfrI CTTAAG 1 cut(s) 101
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 1 cut(s) 416
BlpI GCTNAGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 417
Bme1390I CCNGG 2 cut(s) 180, 219
BmgBI CACGTC 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 3 cut(s) 255, 333, 712
BmrFI CCNGG 2 cut(s) 180, 219
BmsI GCATC 1 cut(s) 80
BpiI GAAGAC 2 cut(s) 11, 99
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 2 cut(s) 20, 26
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 180
BsaJI CCNNGG 7 cut(s) 81, 111, 217, 249, 439, 543, 714
BsaXI ACNNNNNCTCC 2 cut(s) 812, 842
Bse118I RCCGGY 2 cut(s) 404, 418
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 7 cut(s) 81, 111, 217, 249, 439, 543, 714
BseGI GGATG 1 cut(s) 754
BseMII CTCAG 3 cut(s) 605, 770, 788
BseXI GCAGC 1 cut(s) 427
BseYI CCCAGC 1 cut(s) 785
BshFI GGCC 5 cut(s) 80, 245, 368, 404, 624
BshNI GGYRCC 2 cut(s) 253, 710
BsiSI CCGG 3 cut(s) 180, 405, 419
BslFI GGGAC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 5 cut(s) 80, 245, 368, 404, 624
Bsp119I TTCGAA 2 cut(s) 20, 26
Bsp143I GATC 2 cut(s) 51, 331
Bsp1720I GCTNAGC 1 cut(s) 779
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 3 cut(s) 532, 746, 776
BspANI GGCC 5 cut(s) 80, 245, 368, 404, 624
BspCNI CTCAG 3 cut(s) 604, 771, 789
BspLI GGNNCC 3 cut(s) 255, 333, 712
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 3 cut(s) 59, 326, 339
BspT104I TTCGAA 2 cut(s) 20, 26
BspT107I GGYRCC 2 cut(s) 253, 710
BspTI CTTAAG 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 404, 418
BssAI RCCGGY 2 cut(s) 404, 418
BssECI CCNNGG 7 cut(s) 81, 111, 217, 249, 439, 543, 714
BssMI GATC 2 cut(s) 51, 331
BssNAI GTATAC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 6 cut(s) 81, 111, 249, 439, 543, 714
Bst1107I GTATAC 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 219
Bst6I CTCTTC 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 101
BstBI TTCGAA 2 cut(s) 20, 26
BstC8I GCNNGC 2 cut(s) 258, 406
BstDEI CTNAG 3 cut(s) 591, 779, 797
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 1 cut(s) 754
BstH2I RGCGCY 1 cut(s) 786
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 2 cut(s) 54, 334
BstMBI GATC 2 cut(s) 51, 331
BstMWI GCNNNNNNNGC 1 cut(s) 405
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 178, 217
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 427
BstV2I GAAGAC 2 cut(s) 11, 99
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZ17I GTATAC 1 cut(s) 476
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 5 cut(s) 80, 245, 368, 404, 624
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 554
BtsCI GGATG 1 cut(s) 754
Cac8I GCNNGC 2 cut(s) 258, 406
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 1 cut(s) 785
Cfr10I RCCGGY 2 cut(s) 404, 418
Cfr13I GGNCC 1 cut(s) 367
CviAII CATG 3 cut(s) 400, 467, 715
DdeI CTNAG 3 cut(s) 591, 779, 797
DpnI GATC 2 cut(s) 53, 333
DpnII GATC 2 cut(s) 51, 331
DraI TTTAAA 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 6 cut(s) 81, 111, 249, 439, 543, 714
Eco147I AGGCCT 3 cut(s) 80, 245, 624
Eco32I GATATC 1 cut(s) 773
Eco47III AGCGCT 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 217
EcoRV GATATC 1 cut(s) 773
EcoT14I CCWWGG 6 cut(s) 81, 111, 249, 439, 543, 714
ErhI CCWWGG 6 cut(s) 81, 111, 249, 439, 543, 714
FaeI CATG 3 cut(s) 403, 470, 718
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 233, 265
FaqI GGGAC 1 cut(s) 320
FatI CATG 3 cut(s) 399, 466, 714
FblI GTMKAC 2 cut(s) 475, 550
Fnu4HI GCNGC 1 cut(s) 416
FokI GGATG 1 cut(s) 761
Fsp4HI GCNGC 1 cut(s) 416
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 784
GluI GCNGC 1 cut(s) 416
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 786
HaeIII GGCC 5 cut(s) 80, 245, 368, 404, 624
HapII CCGG 3 cut(s) 180, 405, 419
HhaI GCGC 1 cut(s) 785
Hin1II CATG 3 cut(s) 403, 470, 718
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HincII GTYRAC 1 cut(s) 551
HindII GTYRAC 1 cut(s) 551
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 161, 482
HpaII CCGG 3 cut(s) 180, 405, 419
Hpy166II GTNNAC 3 cut(s) 361, 476, 551
Hpy188I TCNGA 2 cut(s) 318, 579
Hpy188III TCNNGA 4 cut(s) 236, 293, 362, 768
Hpy8I GTNNAC 3 cut(s) 361, 476, 551
Hpy99I CGWCG 1 cut(s) 555
HpyAV CCTTC 5 cut(s) 39, 207, 323, 383, 612
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 3 cut(s) 399, 415, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 405
HpyF3I CTNAG 3 cut(s) 591, 779, 797
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 3 cut(s) 403, 470, 718
HspAI GCGC 1 cut(s) 783
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 51, 331
LmnI GCTCC 4 cut(s) 85, 174, 199, 534
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 3 cut(s) 223, 510, 654
MalI GATC 2 cut(s) 53, 333
MboI GATC 2 cut(s) 51, 331
MboII GAAGA 4 cut(s) 16, 82, 99, 170
MflI RGATCY 1 cut(s) 331
MluCI AATT 6 cut(s) 22, 135, 274, 378, 515, 729
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 7 cut(s) 42, 46, 54, 198, 256, 358, 380
MroNI GCCGGC 1 cut(s) 404
MseI TTAA 4 cut(s) 102, 287, 429, 537
MspA1I CMGCKG 1 cut(s) 778
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 3 cut(s) 180, 405, 419
MspR9I CCNGG 2 cut(s) 180, 219
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 405
NaeI GCCGGC 1 cut(s) 406
NciI CCSGG 1 cut(s) 180
NcoI CCATGG 1 cut(s) 714
NdeII GATC 2 cut(s) 51, 331
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 3 cut(s) 403, 470, 718
NlaIV GGNNCC 3 cut(s) 255, 333, 712
NmuCI GTSAC 1 cut(s) 510
NspI RCATGY 1 cut(s) 470
NspV TTCGAA 2 cut(s) 20, 26
PceI AGGCCT 3 cut(s) 80, 245, 624
PctI GAATGC 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 161, 482
PkrI GCNGC 1 cut(s) 417
PsiI TTATAA 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 3 cut(s) 255, 333, 712
PspPI GGNCC 1 cut(s) 367
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 1 cut(s) 331
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 102, 287, 429, 537
SatI GCNGC 1 cut(s) 416
Sau3AI GATC 2 cut(s) 51, 331
Sau96I GGNCC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 180, 219
SetI ASST 7 cut(s) 19, 196, 352, 531, 556, 604, 696
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 413
SfuI TTCGAA 2 cut(s) 20, 26
SmlI CTYRAG 2 cut(s) 94, 101
SmoI CTYRAG 2 cut(s) 94, 101
Sse9I AATT 6 cut(s) 22, 135, 274, 378, 515, 729
SseBI AGGCCT 3 cut(s) 80, 245, 624
SsiI CCGC 3 cut(s) 532, 746, 776
SspI AATATT 1 cut(s) 835
SspMI CTAG 1 cut(s) 191
StuI AGGCCT 3 cut(s) 80, 245, 624
StyD4I CCNGG 2 cut(s) 178, 217
StyI CCWWGG 6 cut(s) 81, 111, 249, 439, 543, 714
TaiI ACGT 1 cut(s) 556
TaqI TCGA 6 cut(s) 20, 26, 54, 237, 459, 550
TasI AATT 6 cut(s) 22, 135, 274, 378, 515, 729
TfiI GAWTC 2 cut(s) 161, 482
Tru1I TTAA 4 cut(s) 102, 287, 429, 537
Tru9I TTAA 4 cut(s) 102, 287, 429, 537
TseFI GTSAC 1 cut(s) 510
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 510
TspDTI ATGAA 5 cut(s) 17, 83, 171, 227, 745
TspGWI ACGGA 2 cut(s) 601, 619
Vha464I CTTAAG 1 cut(s) 101
XapI RAATTY 1 cut(s) 22
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 475, 550
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.