pycom02g24810

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0022377
Physical Location & Seq
Forward (+)
22914334 .. 22915773
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24810.5

Sequence Viewer

Length: 711 bp
ATGAAGACCCACAAAGCTTCGTATTCGAACGAGTCCTCTAATGGAAGGAGGATCGAAAAGAGGGGAACGAAGAGAAAGGCCTTAGAGCGATGCTCAAGGCTTAATTCTTCCAAGGACATCTTGGAAGTCAAAGGAATTATTGAACAGGATATTAATGAAGATTCTATGCTCCAACTTCCCGGCATTCCTCTAGCTCCTTCCAGCCAGAATATGAACCCTGGAGTTACTTTTGTTCTCGAAAAGGCCTCCTTGGTGCCTGCTTATGTCGGCAAGAAGTACCAGATTTTAAATCCAGAAATACACGGGGACTTCCTTAGGAAAAAGAATATGGATCCTTATAAATACAGACCTGACATTGTTCACGAGGCCCTCCTTCAAATTATGGATACCCGACTTTGCATGGCCGGCAGACTGCAGCCGGTGTATGTTAAAACTGACCAAGGGGTTCTCATCAAAGTCGAACCACATGCTCGTATACCAGAATCATTGGACAAGTTTTGTGCTATGATGTCACAATTATTGCAAAAGCTCCGCATTAAAGCCAAGGGTCGACGTGATACACTCTTGCGTGTGGTTCAGAACCCTTTATCTCAGCATTTACCTTCCAACTCCGTCAAAATAGGCCTTTCCGTCAGTTCGCAAAAGGAAGTTCTGCTACGGGAGTATGTAAATGCTGTCAGCAATAATGCCAACCTTGTTTTTGTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

237

Amino Acids

26.66

Weight (kDa)

9.77

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022377)

Species Orthologous Gene IDs
malus_domestica MD02G1295100.v1.1
prunus_persica Prupe.1G567900_v2.0.a1 Prupe.1G569000_v2.0.a1
pyrus_communis pycom02g24810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 253
AccI GTMKAC 2 cut(s) 475, 550
AciI CCGC 1 cut(s) 532
AclWI GGATC 3 cut(s) 59, 326, 339
AcoI YGGCCR 1 cut(s) 402
AfaI GTAC 1 cut(s) 278
AgsI TTSAA 2 cut(s) 143, 377
AjiI CACGTC 1 cut(s) 554
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 3 cut(s) 17, 194, 529
AluI AGCT 3 cut(s) 17, 194, 529
AlwI GGATC 3 cut(s) 59, 326, 339
AoxI GGCC 5 cut(s) 78, 243, 366, 402, 622
ApeKI GCWGC 1 cut(s) 415
AseI ATTAAT 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 180
AsuII TTCGAA 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 314
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 639, 671
BauI CACGAG 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 219
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 413
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 1 cut(s) 416
BlsI GCNGC 1 cut(s) 417
Bme1390I CCNGG 2 cut(s) 180, 219
BmgBI CACGTC 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 2 cut(s) 255, 333
BmrFI CCNGG 2 cut(s) 180, 219
BmsI GCATC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 111, 217, 249, 439, 543
Bse118I RCCGGY 2 cut(s) 404, 418
Bse21I CCTNAGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 5 cut(s) 111, 217, 249, 439, 543
BseMII CTCAG 1 cut(s) 605
BseXI GCAGC 1 cut(s) 427
BshFI GGCC 5 cut(s) 80, 245, 368, 404, 624
BshNI GGYRCC 1 cut(s) 253
BsiSI CCGG 3 cut(s) 180, 405, 419
BslFI GGGAC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 5 cut(s) 80, 245, 368, 404, 624
Bsp119I TTCGAA 1 cut(s) 26
Bsp143I GATC 2 cut(s) 51, 331
BspACI CCGC 1 cut(s) 532
BspANI GGCC 5 cut(s) 80, 245, 368, 404, 624
BspCNI CTCAG 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 255, 333
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 3 cut(s) 59, 326, 339
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 253
BsrFI RCCGGY 2 cut(s) 404, 418
BssAI RCCGGY 2 cut(s) 404, 418
BssECI CCNNGG 5 cut(s) 111, 217, 249, 439, 543
BssMI GATC 2 cut(s) 51, 331
BssNAI GTATAC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 4 cut(s) 111, 249, 439, 543
Bst1107I GTATAC 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 219
Bst6I CTCTTC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 258, 406
BstDEI CTNAG 3 cut(s) 82, 314, 591
BstKTI GATC 2 cut(s) 54, 334
BstMBI GATC 2 cut(s) 51, 331
BstMWI GCNNNNNNNGC 1 cut(s) 405
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 178, 217
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 427
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZ17I GTATAC 1 cut(s) 476
Bsu36I CCTNAGG 1 cut(s) 314
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 5 cut(s) 80, 245, 368, 404, 624
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 554
Cac8I GCNNGC 2 cut(s) 258, 406
Cfr10I RCCGGY 2 cut(s) 404, 418
Cfr13I GGNCC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 400, 467
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 82, 314, 591
DpnI GATC 2 cut(s) 53, 333
DpnII GATC 2 cut(s) 51, 331
DraI TTTAAA 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 4 cut(s) 111, 249, 439, 543
Eco147I AGGCCT 3 cut(s) 80, 245, 624
Eco81I CCTNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 4 cut(s) 111, 249, 439, 543
ErhI CCWWGG 4 cut(s) 111, 249, 439, 543
FaeI CATG 2 cut(s) 403, 470
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 233, 265
FaqI GGGAC 1 cut(s) 320
FatI CATG 2 cut(s) 399, 466
FblI GTMKAC 2 cut(s) 475, 550
Fnu4HI GCNGC 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 416
FspBI CTAG 1 cut(s) 191
GluI GCNGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 5 cut(s) 80, 245, 368, 404, 624
HapII CCGG 3 cut(s) 180, 405, 419
Hin1II CATG 2 cut(s) 403, 470
HincII GTYRAC 1 cut(s) 551
HindII GTYRAC 1 cut(s) 551
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 3 cut(s) 32, 161, 482
HpaII CCGG 3 cut(s) 180, 405, 419
Hpy166II GTNNAC 3 cut(s) 361, 476, 551
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 3 cut(s) 236, 293, 362
Hpy8I GTNNAC 3 cut(s) 361, 476, 551
Hpy99I CGWCG 1 cut(s) 555
HpyAV CCTTC 4 cut(s) 39, 207, 383, 612
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 3 cut(s) 399, 415, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 405
HpyF3I CTNAG 3 cut(s) 82, 314, 591
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 2 cut(s) 403, 470
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 51, 331
LmnI GCTCC 3 cut(s) 174, 199, 534
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 2 cut(s) 223, 510
MalI GATC 2 cut(s) 53, 333
MboI GATC 2 cut(s) 51, 331
MboII GAAGA 4 cut(s) 16, 82, 99, 170
MflI RGATCY 1 cut(s) 331
MluCI AATT 4 cut(s) 103, 135, 378, 515
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 196, 630
MnlI CCTC 7 cut(s) 42, 46, 54, 198, 256, 358, 380
MroNI GCCGGC 1 cut(s) 404
MseI TTAA 5 cut(s) 102, 153, 287, 429, 537
MspI CCGG 3 cut(s) 180, 405, 419
MspR9I CCNGG 2 cut(s) 180, 219
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 405
NaeI GCCGGC 1 cut(s) 406
NciI CCSGG 1 cut(s) 180
NdeII GATC 2 cut(s) 51, 331
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 2 cut(s) 403, 470
NlaIV GGNNCC 2 cut(s) 255, 333
NmuCI GTSAC 1 cut(s) 510
NspI RCATGY 1 cut(s) 470
NspV TTCGAA 1 cut(s) 26
PceI AGGCCT 3 cut(s) 80, 245, 624
PctI GAATGC 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 161, 482
PkrI GCNGC 1 cut(s) 417
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 153
PsiI TTATAA 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 255, 333
PspPI GGNCC 1 cut(s) 367
PstI CTGCAG 1 cut(s) 417
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 5 cut(s) 102, 153, 287, 429, 537
SatI GCNGC 1 cut(s) 416
Sau3AI GATC 2 cut(s) 51, 331
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 180, 219
SetI ASST 7 cut(s) 19, 196, 352, 531, 556, 604, 696
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 4 cut(s) 103, 135, 378, 515
SseBI AGGCCT 3 cut(s) 80, 245, 624
SsiI CCGC 1 cut(s) 532
SspMI CTAG 1 cut(s) 191
StuI AGGCCT 3 cut(s) 80, 245, 624
StyD4I CCNGG 2 cut(s) 178, 217
StyI CCWWGG 4 cut(s) 111, 249, 439, 543
TaiI ACGT 1 cut(s) 556
TaqI TCGA 5 cut(s) 26, 54, 237, 459, 550
TasI AATT 4 cut(s) 103, 135, 378, 515
TfiI GAWTC 2 cut(s) 161, 482
Tru1I TTAA 5 cut(s) 102, 153, 287, 429, 537
Tru9I TTAA 5 cut(s) 102, 153, 287, 429, 537
TseFI GTSAC 1 cut(s) 510
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 510
TspDTI ATGAA 3 cut(s) 17, 171, 227
TspGWI ACGGA 2 cut(s) 601, 619
VspI ATTAAT 1 cut(s) 153
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 475, 550
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.