Prupe.1G569000_v2.0.a1

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0022377
Physical Location & Seq
Reverse (-)
46390835 .. 46392764
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G569000.1

Sequence Viewer

Length: 543 bp
ATGCTGACTTCTTTGCGGAGAAAGAAAATGGATCCTTATAAATACAGACCCGACATTGTTCATGAGGCTCTCCGTCGAATCATGGATACCCGACTTTGCAAGGCTGGTAGACTACATGCTGTGTATATTAGAACTGATGAAGGGGTGCTCATTAAAGTTGAACCACGTGCTACTATACCAGAATCGTTGGAGAAGTTCTGTTGTATGATGTCACAATTATTGCAAAAGTTCAGCATTAAATCCACGGGTGGACGTGGAAGTCTCTTGCGTGTGGTTAAGAACCCTGTAGCTCAGCATTTACCTGCCAATTCCCTCAAGATAGGTCTTTCTTTGAGTTCGCAAAAGGCAGTTGACTTACGGGATTATGTACATGATGTCAGCGATAATGCCAACCTTGTTTTCGTGGTTGGTGCAATGGCTCACGGGAGAATTGAAAGCGAGAATGTGGATGATATCATATCAGTTTCTGATTATCCGTTGAGTGCTGTGGTTTGCCTGGAAAGGATAAGTATGGCATTAGAGAGAAAGTGGAACATTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

181

Amino Acids

20.29

Weight (kDa)

9.36

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022377)

Species Orthologous Gene IDs
malus_domestica MD02G1295100.v1.1
prunus_persica Prupe.1G567900_v2.0.a1 Prupe.1G569000_v2.0.a1
pyrus_communis pycom02g24810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
AasI GACNNNNNNGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 109
AciI CCGC 1 cut(s) 16
AclWI GGATC 2 cut(s) 26, 39
AcvI CACGTG 1 cut(s) 167
AfaI GTAC 1 cut(s) 369
AgsI TTSAA 2 cut(s) 161, 434
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 1 cut(s) 495
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 2 cut(s) 26, 39
AlwNI CAGNNNCTG 1 cut(s) 467
BamHI GGATCC 1 cut(s) 31
BbrPI CACGTG 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 79
BlpI GCTNAGC 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 497
Bpu1102I GCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 243
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 454
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 150
BsmAI GTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 1 cut(s) 31
Bsp1720I GCTNAGC 1 cut(s) 291
BspACI CCGC 1 cut(s) 16
BspCNI CTCAG 1 cut(s) 304
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 26, 39
BsrDI GCAATG 1 cut(s) 420
BsrGI TGTACA 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 167
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 454
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 495
BstSFI CTRYAG 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuI GTATCC 1 cut(s) 79
BtgI CCRYGG 1 cut(s) 243
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 1 cut(s) 454
BveI ACCTGC 1 cut(s) 310
CaiI CAGNNNCTG 1 cut(s) 467
CciI TCATGA 1 cut(s) 61
Csp6I GTAC 1 cut(s) 368
CviAII CATG 4 cut(s) 62, 82, 116, 371
CviJI RGCY 4 cut(s) 68, 104, 290, 419
CviKI_1 RGCY 4 cut(s) 68, 104, 290, 419
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 1 cut(s) 291
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DrdI GACNNNNNNGTC 1 cut(s) 258
DseDI GACNNNNNNGTC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 454
Eco72I CACGTG 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 454
FaeI CATG 4 cut(s) 65, 85, 119, 374
FatI CATG 4 cut(s) 61, 81, 115, 370
FblI GTMKAC 1 cut(s) 109
FokI GGATG 1 cut(s) 461
Hin1II CATG 4 cut(s) 65, 85, 119, 374
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 2 cut(s) 78, 182
Hpy166II GTNNAC 3 cut(s) 110, 251, 352
Hpy188I TCNGA 1 cut(s) 469
Hpy188III TCNNGA 2 cut(s) 62, 316
Hpy8I GTNNAC 3 cut(s) 110, 251, 352
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 1 cut(s) 134
HpyCH4IV ACGT 2 cut(s) 166, 253
HpyCH4V TGCA 3 cut(s) 99, 223, 413
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 2 cut(s) 166, 253
Hsp92II CATG 4 cut(s) 65, 85, 119, 374
Kzo9I GATC 1 cut(s) 31
LpnPI CCDG 6 cut(s) 90, 192, 297, 315, 482, 509
MaeII ACGT 2 cut(s) 166, 253
MaeIII GTNAC 1 cut(s) 210
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 3 cut(s) 215, 307, 429
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 2 cut(s) 58, 323
MseI TTAA 3 cut(s) 153, 237, 276
MspR9I CCNGG 1 cut(s) 497
MvaI CCWGG 1 cut(s) 497
NdeII GATC 1 cut(s) 31
NlaIII CATG 4 cut(s) 65, 85, 119, 374
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 210
NspI RCATGY 1 cut(s) 119
PagI TCATGA 1 cut(s) 61
PfeI GAWTC 2 cut(s) 78, 182
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 167
PsiI TTATAA 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 495
PspCI CACGTG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 153, 237, 276
Sau3AI GATC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 1 cut(s) 150
SetI ASST 6 cut(s) 169, 256, 292, 304, 325, 396
SfcI CTRYAG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 3 cut(s) 215, 307, 429
SsiI CCGC 1 cut(s) 16
StyD4I CCNGG 1 cut(s) 495
TaiI ACGT 2 cut(s) 169, 256
TaqI TCGA 1 cut(s) 76
TasI AATT 3 cut(s) 215, 307, 429
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 2 cut(s) 78, 182
Tru1I TTAA 3 cut(s) 153, 237, 276
Tru9I TTAA 3 cut(s) 153, 237, 276
TseFI GTSAC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 210
TspDTI ATGAA 2 cut(s) 50, 153
TspGWI ACGGA 2 cut(s) 62, 465
XceI RCATGY 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.