MD02G1318300.v1.1

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017386
Physical Location & Seq
Forward (+)
37274564 .. 37277519
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1318300.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGCCTCTTGCAGGTACTTATTCAACTCTTGCATTCAGACTTATCACGTGCTTGTTTTTAGAGAAAAACATCCTAGTAAGACCAGACGCTATTCAGTAACGAGATGTAAAAGTAGTTGCCGTCGAACTTCAGATGAAGAGAAATGTACCACAAGGAAGCTTAAAATTCTAATTGCTGGTGGGGGTATTGGTGGGTTAGTTTTGGCGTTGGCAGCAAAACGCCGAGGGTTTGAAGTGCAGGTGTTCGAGAAGGATCTGAGTGCAGTGAGAGGGGAGGGACAACACTGCGGTCCAATTCAACTTGTAAGTAGTGCTTTGGCAGTGCTGGAAGCCATTGATGAGAATGTTGCAAAGCAAATCATGGGTGCAGGTTGTGTTACTGGCAATAGGACCACTGGCTATGCCGACGGTCATTCAGGCGAATGGATTACAAAGTTTGATCTTTCCTCCTCAGCTTTGAGTAAGGGCCTTCCCCTCACTATCATAATATGCAGGATGGCGCTGCAAGATATCTTAGTCAATGCAGTTGGGACTGAAATTGTGAGAAACAAATCCAAAGTGGTGGACTTCATTCAAGACCCAAGCAAGGTTACAGCTATCCTTGAAGGCGGGGAACAATTTGATGGTGATGTTTTAGTAGGAGCTGATGGAATTTGGTCAACAGTCCGTTCGAAATTGTTTGGGAGACAGGAAGCAAAATATTCGAGTTACGCATCCTACAGTGGAGTGACAAACTTTGTGCCACCATATATTGATAGCGTTGCGTACCGGATCTTCATCGGATTGAACCAGTGCTTTGTTGCAACAGATGTTGGGGGTGGGAAGGTACAATGGTTTGCCAATCATAGAGACCAGCCTATCAGCAATGAACCTCCTCAAGGAACACTCATCTCTCCCACTTTTCTCTATGCTGCAGGTAGAAAGAAGATGCTCCTGGAGATATTTGGTAATTGGTGTCCTGAAGTGGTTGCAATAATTCAGGAAACAGCAGAGTCCACAATTTTACGGCGAGCTATCTATGATAGAGACATGATTTACACCTGGGGAACCGGCCGTGTTACTCTGCTGGGTGATGCTGCTCATCCCATGCAGCCGAATCTAGGTCAAGGGGGTTGCATGGCAATTGAGGAAAACCCTGTCTCTAGCAAAGCAATCTCACTCCTAGTTTTAGGCTTGAGTATTGTTGAAATCTTCCAAATTTACAAAATTTTGTCTAAATTGAAAGATCTTCAAAACTCACTTGTAAGTTTTGCGTTCATGATGACGATGGTGAAGATCAGATATGTAAAGAAAAGAATGTGGCGTGTTGGCATAGTGCACGCAGCCTCCCGAATGGCATCAAGAATGCTTGAAAGTTACCAACCTTACATAAAGTTTCGAACCGGTCCTGTGGCTCATCTACTGACTCGGCAGATAACACACCCTGCAATTCCTCTATTTCGTGCATTTCTTCAATTTTTTATGCCAAAGTTTATGGCTTGGATGATAGCAGGGGATGGGTTATTTCTCAAAGGGGAGAGAACTGAATGCAAAGTGCAGGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

516

Amino Acids

57.16

Weight (kDa)

9.4

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 54 - 248 7.6e-10 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 4 cut(s) 3, 229, 359, 905
AciI CCGC 2 cut(s) 288, 609
AclWI GGATC 2 cut(s) 261, 779
AcoI YGGCCR 1 cut(s) 1051
AcsI RAATTY 4 cut(s) 165, 651, 1197, 1206
AcuI CTGAAG 2 cut(s) 114, 981
AcvI CACGTG 1 cut(s) 49
AfaI GTAC 4 cut(s) 17, 148, 767, 828
AfiI CCNNNNNNNGG 4 cut(s) 12, 586, 878, 1100
AgeI ACCGGT 1 cut(s) 1382
AjnI CCWGG 2 cut(s) 933, 1040
AluBI AGCT 5 cut(s) 160, 455, 596, 644, 1013
AluI AGCT 5 cut(s) 160, 455, 596, 644, 1013
Alw21I GWGCWC 1 cut(s) 1320
Alw26I GTCTC 4 cut(s) 679, 843, 1020, 1144
Alw44I GTGCAC 1 cut(s) 1316
AlwI GGATC 2 cut(s) 261, 779
AoxI GGCC 3 cut(s) 3, 466, 1051
ApaLI GTGCAC 1 cut(s) 1316
ApeKI GCWGC 6 cut(s) 212, 502, 911, 1076, 1090, 1322
ApoI RAATTY 4 cut(s) 165, 651, 1197, 1206
AsiGI ACCGGT 1 cut(s) 1382
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 4 cut(s) 290, 390, 466, 1385
AsuHPI GGTGA 3 cut(s) 638, 1082, 1282
AsuII TTCGAA 2 cut(s) 671, 1378
AvaII GGWCC 3 cut(s) 290, 390, 1385
BaeGI GKGCMC 1 cut(s) 1320
BaeI ACNNNNGTAYC 2 cut(s) 818, 851
BarI GAAGNNNNNNTAC 2 cut(s) 758, 790
BbrPI CACGTG 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 1320
BbvCI CCTCAGC 1 cut(s) 451
BbvI GCAGC 6 cut(s) 224, 489, 898, 1063, 1102, 1334
BccI CCATC 5 cut(s) 490, 617, 641, 1261, 1490
BceAI ACGGC 3 cut(s) 105, 1022, 1038
BcgI CGANNNNNNTGC 2 cut(s) 684, 718
BciT130I CCWGG 2 cut(s) 935, 1042
BcoDI GTCTC 4 cut(s) 679, 843, 1020, 1144
BfaI CTAG 4 cut(s) 75, 1100, 1143, 1163
BfmI CTRYAG 2 cut(s) 718, 912
BfoI RGCGCY 1 cut(s) 503
BfuAI ACCTGC 4 cut(s) 3, 229, 359, 905
BglII AGATCT 1 cut(s) 1225
BisI GCNGC 6 cut(s) 213, 503, 912, 1077, 1091, 1323
BlsI GCNGC 6 cut(s) 214, 504, 913, 1078, 1092, 1324
Bme1390I CCNGG 2 cut(s) 935, 1042
Bme18I GGWCC 3 cut(s) 290, 390, 1385
BmgT120I GGNCC 4 cut(s) 290, 390, 466, 1385
BmiI GGNNCC 1 cut(s) 1048
BmrFI CCNGG 2 cut(s) 935, 1042
BmsI GCATC 4 cut(s) 722, 918, 1063, 1346
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 451
Bpu14I TTCGAA 2 cut(s) 671, 1378
BpuEI CTTGAG 2 cut(s) 861, 1195
BsaAI YACGTR 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 776
BsaI GGTCTC 1 cut(s) 843
BsaJI CCNNGG 2 cut(s) 223, 1041
BsaWI WCCGGW 2 cut(s) 768, 1382
BsaXI ACNNNNNCTCC 2 cut(s) 1310, 1340
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 586, 878, 1100
Bse118I RCCGGY 2 cut(s) 1049, 1382
Bse1I ACTGG 3 cut(s) 385, 400, 790
Bse3DI GCAATG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 776
BseBI CCWGG 2 cut(s) 935, 1042
BseDI CCNNGG 2 cut(s) 223, 1041
BseGI GGATG 6 cut(s) 70, 501, 713, 1081, 1488, 1501
BseJI GATNNNNATC 1 cut(s) 776
BseLI CCNNNNNNNGG 4 cut(s) 12, 586, 878, 1100
BseMI GCAATG 1 cut(s) 871
BseMII CTCAG 2 cut(s) 248, 465
BseNI ACTGG 3 cut(s) 385, 400, 790
BseRI GAGGAG 2 cut(s) 439, 864
BseSI GKGCMC 1 cut(s) 1320
BseX3I CGGCCG 1 cut(s) 1051
BseXI GCAGC 6 cut(s) 224, 489, 898, 1063, 1102, 1334
BseYI CCCAGC 1 cut(s) 1066
BsgI GTGCAG 3 cut(s) 257, 282, 387
Bsh1285I CGRYCG 1 cut(s) 1054
BshFI GGCC 3 cut(s) 5, 468, 1053
BshTI ACCGGT 1 cut(s) 1382
BsiEI CGRYCG 1 cut(s) 1054
BsiHKAI GWGCWC 1 cut(s) 1320
BsiSI CCGG 3 cut(s) 769, 1050, 1383
BslFI GGGAC 2 cut(s) 291, 544
BslI CCNNNNNNNGG 4 cut(s) 12, 586, 878, 1100
BsmAI GTCTC 4 cut(s) 679, 843, 1020, 1144
BsmFI GGGAC 2 cut(s) 291, 544
BsmI GAATGC 3 cut(s) 33, 1350, 1532
BsnI GGCC 3 cut(s) 5, 468, 1053
Bso31I GGTCTC 1 cut(s) 843
Bsp119I TTCGAA 2 cut(s) 671, 1378
Bsp1286I GDGCHC 1 cut(s) 1320
Bsp143I GATC 5 cut(s) 253, 439, 771, 1225, 1275
BspACI CCGC 2 cut(s) 288, 609
BspANI GGCC 3 cut(s) 5, 468, 1053
BspCNI CTCAG 2 cut(s) 249, 464
BspHI TCATGA 1 cut(s) 1257
BspLI GGNNCC 1 cut(s) 1048
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 4 cut(s) 3, 229, 359, 905
BspPI GGATC 2 cut(s) 261, 779
BspT104I TTCGAA 2 cut(s) 671, 1378
BspTNI GGTCTC 1 cut(s) 843
BsrDI GCAATG 1 cut(s) 871
BsrFI RCCGGY 2 cut(s) 1049, 1382
BsrI ACTGG 3 cut(s) 385, 400, 790
BssAI RCCGGY 2 cut(s) 1049, 1382
BssECI CCNNGG 2 cut(s) 223, 1041
BssMI GATC 5 cut(s) 253, 439, 771, 1225, 1275
Bst2UI CCWGG 2 cut(s) 935, 1042
Bst4CI ACNGT 3 cut(s) 410, 664, 722
Bst6I CTCTTC 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 49
BstBI TTCGAA 2 cut(s) 671, 1378
BstC8I GCNNGC 2 cut(s) 1011, 1320
BstDEI CTNAG 3 cut(s) 257, 451, 514
BstENI CCTNNNNNAGG 2 cut(s) 10, 876
BstF5I GGATG 6 cut(s) 70, 501, 713, 1081, 1488, 1501
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 5 cut(s) 256, 442, 774, 1228, 1278
BstMAI GTCTC 4 cut(s) 679, 843, 1020, 1144
BstMBI GATC 5 cut(s) 253, 439, 771, 1225, 1275
BstMCI CGRYCG 1 cut(s) 1054
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 2 cut(s) 935, 1042
BstSCI CCNGG 2 cut(s) 933, 1040
BstSFI CTRYAG 2 cut(s) 718, 912
BstSLI GKGCMC 1 cut(s) 1320
BstV1I GCAGC 6 cut(s) 224, 489, 898, 1063, 1102, 1334
BstX2I RGATCY 3 cut(s) 253, 771, 1225
BstXI CCANNNNNNTGG 1 cut(s) 562
BstYI RGATCY 3 cut(s) 253, 771, 1225
BstZI CGGCCG 1 cut(s) 1051
BsuRI GGCC 3 cut(s) 5, 468, 1053
BtsCI GGATG 6 cut(s) 70, 501, 713, 1081, 1488, 1501
BtsI GCAGTG 3 cut(s) 270, 283, 327
BtsIMutI CAGTG 6 cut(s) 270, 283, 327, 393, 727, 797
BveI ACCTGC 4 cut(s) 3, 229, 359, 905
Cac8I GCNNGC 2 cut(s) 1011, 1320
CciI TCATGA 1 cut(s) 1257
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 2 cut(s) 1049, 1382
Cfr13I GGNCC 4 cut(s) 290, 390, 466, 1385
CseI GACGC 1 cut(s) 96
Csp6I GTAC 4 cut(s) 16, 147, 766, 827
CspAI ACCGGT 1 cut(s) 1382
CviAII CATG 5 cut(s) 361, 1030, 1087, 1117, 1258
CviQI GTAC 4 cut(s) 16, 147, 766, 827
DdeI CTNAG 3 cut(s) 257, 451, 514
DpnI GATC 5 cut(s) 255, 441, 773, 1227, 1277
DpnII GATC 5 cut(s) 253, 439, 771, 1225, 1275
EaeI YGGCCR 1 cut(s) 1051
EagI CGGCCG 1 cut(s) 1051
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 1051
Eco31I GGTCTC 1 cut(s) 843
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 3 cut(s) 290, 390, 1385
Eco52I CGGCCG 1 cut(s) 1051
Eco57I CTGAAG 2 cut(s) 114, 981
Eco72I CACGTG 1 cut(s) 49
EcoNI CCTNNNNNAGG 2 cut(s) 10, 876
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 933, 1040
EcoRV GATATC 1 cut(s) 511
FaeI CATG 5 cut(s) 364, 1033, 1090, 1120, 1261
FalI AAGNNNNNCTT 2 cut(s) 298, 330
FaqI GGGAC 2 cut(s) 291, 544
FatI CATG 5 cut(s) 360, 1029, 1086, 1116, 1257
FauI CCCGC 1 cut(s) 602
Fnu4HI GCNGC 6 cut(s) 213, 503, 912, 1077, 1091, 1323
FokI GGATG 6 cut(s) 57, 508, 700, 1068, 1495, 1508
Fsp4HI GCNGC 6 cut(s) 213, 503, 912, 1077, 1091, 1323
FspBI CTAG 4 cut(s) 75, 1100, 1143, 1163
GlaI GCGC 1 cut(s) 501
GluI GCNGC 6 cut(s) 213, 503, 912, 1077, 1091, 1323
GsaI CCCAGC 1 cut(s) 1070
GsuI CTGGAG 1 cut(s) 956
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 3 cut(s) 5, 468, 1053
HapII CCGG 3 cut(s) 769, 1050, 1383
HgaI GACGC 1 cut(s) 96
HhaI GCGC 1 cut(s) 502
Hin1II CATG 5 cut(s) 364, 1033, 1090, 1120, 1261
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 3 cut(s) 992, 1096, 1405
HpaII CCGG 3 cut(s) 769, 1050, 1383
HphI GGTGA 3 cut(s) 638, 1082, 1282
Hpy166II GTNNAC 4 cut(s) 565, 660, 996, 1318
Hpy188I TCNGA 5 cut(s) 39, 133, 258, 782, 1280
Hpy188III TCNNGA 7 cut(s) 247, 575, 959, 980, 1258, 1329, 1341
Hpy8I GTNNAC 4 cut(s) 565, 660, 996, 1318
Hpy99I CGWCG 2 cut(s) 126, 410
HpyAV CCTTC 4 cut(s) 244, 479, 599, 817
HpyCH4III ACNGT 3 cut(s) 410, 664, 722
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 3 cut(s) 257, 451, 514
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 5 cut(s) 364, 1033, 1090, 1120, 1261
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 5 cut(s) 253, 439, 771, 1225, 1275
LmnI GCTCC 2 cut(s) 641, 936
Lsp1109I GCAGC 6 cut(s) 224, 489, 898, 1063, 1102, 1334
LweI GCATC 4 cut(s) 722, 918, 1063, 1346
MaeI CTAG 4 cut(s) 75, 1100, 1143, 1163
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 7 cut(s) 97, 376, 589, 707, 727, 1057, 1355
MalI GATC 5 cut(s) 255, 441, 773, 1227, 1277
MboI GATC 5 cut(s) 253, 439, 771, 1225, 1275
MboII GAAGA 7 cut(s) 149, 766, 937, 1183, 1220, 1285, 1442
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 3 cut(s) 253, 771, 1225
MhlI GDGCHC 1 cut(s) 1320
MlyI GAGTC 2 cut(s) 1001, 1399
MseI TTAA 1 cut(s) 162
MspI CCGG 3 cut(s) 769, 1050, 1383
MspR9I CCNGG 2 cut(s) 935, 1042
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 3 cut(s) 33, 1350, 1532
MvaI CCWGG 2 cut(s) 935, 1042
MwoI GCNNNNNNNGC 1 cut(s) 212
NdeII GATC 5 cut(s) 253, 439, 771, 1225, 1275
NlaIII CATG 5 cut(s) 364, 1033, 1090, 1120, 1261
NlaIV GGNNCC 1 cut(s) 1048
NmeAIII GCCGAG 2 cut(s) 248, 1387
NmuCI GTSAC 1 cut(s) 727
NspV TTCGAA 2 cut(s) 671, 1378
PagI TCATGA 1 cut(s) 1257
PaqCI CACCTGC 1 cut(s) 229
PctI GAATGC 3 cut(s) 33, 1350, 1532
PfeI GAWTC 1 cut(s) 1096
PfoI TCCNGGA 1 cut(s) 933
PinAI ACCGGT 1 cut(s) 1382
PkrI GCNGC 6 cut(s) 214, 504, 913, 1078, 1092, 1324
PleI GAGTC 2 cut(s) 1000, 1399
PmaCI CACGTG 1 cut(s) 49
PmlI CACGTG 1 cut(s) 49
PpsI GAGTC 2 cut(s) 1000, 1399
Ppu21I YACGTR 1 cut(s) 49
Psp6I CCWGG 2 cut(s) 933, 1040
PspCI CACGTG 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 1066
PspGI CCWGG 2 cut(s) 933, 1040
PspN4I GGNNCC 1 cut(s) 1048
PspPI GGNCC 4 cut(s) 290, 390, 466, 1385
PstI CTGCAG 1 cut(s) 916
PsuI RGATCY 3 cut(s) 253, 771, 1225
RsaI GTAC 4 cut(s) 17, 148, 767, 828
RsaNI GTAC 4 cut(s) 16, 147, 766, 827
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 6 cut(s) 213, 503, 912, 1077, 1091, 1323
Sau3AI GATC 5 cut(s) 253, 439, 771, 1225, 1275
Sau96I GGNCC 4 cut(s) 290, 390, 466, 1385
SchI GAGTC 2 cut(s) 1001, 1399
ScrFI CCNGG 2 cut(s) 935, 1042
SduI GDGCHC 1 cut(s) 1320
SfaNI GCATC 4 cut(s) 722, 918, 1063, 1346
SfcI CTRYAG 2 cut(s) 718, 912
SfuI TTCGAA 2 cut(s) 671, 1378
SinI GGWCC 3 cut(s) 290, 390, 1385
SmlI CTYRAG 2 cut(s) 876, 1174
SmoI CTYRAG 2 cut(s) 876, 1174
SsiI CCGC 2 cut(s) 288, 609
SspI AATATT 1 cut(s) 701
SspMI CTAG 4 cut(s) 75, 1100, 1143, 1163
StyD4I CCNGG 2 cut(s) 933, 1040
TaaI ACNGT 3 cut(s) 410, 664, 722
TaiI ACGT 1 cut(s) 51
TaqI TCGA 5 cut(s) 124, 246, 671, 704, 1378
TfiI GAWTC 1 cut(s) 1096
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 6 cut(s) 270, 290, 327, 400, 727, 797
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 6 cut(s) 212, 502, 911, 1076, 1090, 1322
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 5 cut(s) 150, 559, 766, 882, 1246
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 6 cut(s) 270, 290, 327, 400, 727, 797
VneI GTGCAC 1 cut(s) 1316
VpaK11BI GGWCC 3 cut(s) 290, 390, 1385
XagI CCTNNNNNAGG 2 cut(s) 10, 876
XapI RAATTY 4 cut(s) 165, 651, 1197, 1206
XspI CTAG 4 cut(s) 75, 1100, 1143, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.