MD07G1003000.v1.1

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017386
Physical Location & Seq
Reverse (-)
308829 .. 312425
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1003000.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGGCGGTCTTTTCTCTGTATCTTAGTTCATGTCATCAGAGTCTTGGGTTTAGAGCAAAAAAATACAGTAAGACTAGGATCATCAGAGGCAACAGTGTAATAAGATGTAAAAGTAGTAGATTAGATCAAACTTCGGACGAAGAGAAGGGTGGCAAAAGGAAGCTTAAAATTCTGATCGCCGGTGGGGGCATTGGGGGGCTAGTTTTGGCATTGGCAGCGAAACACCGAGGATTCCAAGTGCAGGTGTTTGAGAAAGACTCGAGTTCAGTGAGAGGGGAGGGGAAAGGACGTGGACCAATTCAACTTGTGAGTAGTGCATTGGCAGTGCTGGAAGCCATTGACGAGGACGTTGTGAAACAAATCATGGAAGCAGGTTGTGATACTGGCAACAGAATCAATGGCTATGCTGACGGAGTCTCAGGTGAATGGTTTACCAAGTTTGATCTGTCATCGCCGGCTGTAAGTAGGGGGCTTCCTATCACTCAAGTAATATGCAGGATGGAGCTTCTGGATATCTTAGTCAATGCAGTTGGATCTGACATTCTGAGAAACAAATCAAAAGTGGTTGACTTCATTCAAGACCCTAACAAGGTTGAGTGGCATTCGATTCAACTAATCCTAACATATTGGTGCATTGGTCTTTGCTGCCTTAATGCTTTTGCTGGGAGTCTCGTTACAGTGATCCTTGAAGATGGACAACGATATGATGGTGATGTTTTAGTAGGAGCCGATGGAATCTGGTCAAAGGTGCGCTCGAAATTGTTTGGCATACGGGAAGCGAGATACTCAAATTACACCTGCTACAGTGGACTCACTAAAGTTGTCCCATCATATGTTAATAGTGTTGGGTATCGGATCTTCTTGGGAGCGAACCAGTACTTTGGTGCAGTAGAAGTTGGGCATGGGAATATGCAATGGTTGGCCTTCCATAAGCAGCCGCCAATGAGCACTGATCCTCCTGAAGGCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTTGTGGTAAAAAGAAGAGGCTCCTGGAGGTATTTGGAAAATGGTGTCAGGAAGTGGTTGCTCTAATTCAAGAAACACCTGAGTCCATGATTTTACAAAGAGATATCTTCGATAGAGACATGATATATTCCTGGGGAACCGGGCGTGTTGCTCTGCTAGGTGATGCTGCTCATCCATTGCAGCCAAATCTTGGAATAGGGGGATGCATGGCAGTTGAGGACAGTTACCAGCTTGTAGATGAGCTTGATCAAGTTTCCAGTACTGGCTCCGATGCTCAAAGGACAGATGCAATTGTCTTGGCCCTGAGAAGATATGCAACAAAAAGAATGAGGCGTGTTGGTATAGTGCATGCAGCCACTCGAATGGCACCGGAAATGCTTGCTATGTACCGACCTTGCATAGAGTTCAAAATTGGTCCCCTGGCTCATCTATCAACTCTGAAGATAATGCACCCTGCATTTCCGATGGCTCGCGCATTTCTGCAATTTTGTATGCCAGAATTTATGACTTGGATAATAACAGGGCACGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

510

Amino Acids

56.09

Weight (kDa)

8.84

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 232, 806
Acc36I ACCTGC 3 cut(s) 232, 362, 806
AccB1I GGYRCC 1 cut(s) 1363
AccB7I CCANNNNNTGG 1 cut(s) 1187
AccII CGCG 1 cut(s) 1470
AciI CCGC 2 cut(s) 5, 938
AclWI GGATC 5 cut(s) 86, 541, 676, 863, 947
AcsI RAATTY 2 cut(s) 168, 1496
AcuI CTGAAG 2 cut(s) 981, 1457
AfaI GTAC 3 cut(s) 878, 1258, 1385
AfiI CCNNNNNNNGG 4 cut(s) 241, 589, 962, 1187
AgsI TTSAA 6 cut(s) 302, 578, 611, 689, 1067, 1405
AjiI CACGTC 1 cut(s) 290
AjnI CCWGG 3 cut(s) 1020, 1127, 1416
AluBI AGCT 4 cut(s) 163, 505, 1228, 1240
AluI AGCT 4 cut(s) 163, 505, 1228, 1240
Alw21I GWGCWC 1 cut(s) 950
Alw26I GTCTC 3 cut(s) 421, 674, 1107
AlwI GGATC 5 cut(s) 86, 541, 676, 863, 947
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 2 cut(s) 921, 1296
ApeKI GCWGC 6 cut(s) 215, 645, 934, 1163, 1177, 1349
ApoI RAATTY 2 cut(s) 168, 1496
AspLEI GCGC 2 cut(s) 753, 1472
AspS9I GGNCC 3 cut(s) 293, 1297, 1412
AsuC2I CCSGG 1 cut(s) 1138
AsuHPI GGTGA 3 cut(s) 434, 722, 1169
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 2 cut(s) 293, 1412
BaeGI GKGCMC 1 cut(s) 1524
BanI GGYRCC 1 cut(s) 1363
BarI GAAGNNNNNNTAC 2 cut(s) 842, 874
Bbv12I GWGCWC 1 cut(s) 950
BbvI GCAGC 6 cut(s) 227, 632, 946, 1150, 1189, 1361
BccI CCATC 6 cut(s) 493, 686, 701, 725, 835, 1456
BciT130I CCWGG 3 cut(s) 1022, 1129, 1418
BclI TGATCA 1 cut(s) 1243
BcnI CCSGG 1 cut(s) 1138
BcoDI GTCTC 3 cut(s) 421, 674, 1107
BfaI CTAG 3 cut(s) 75, 200, 1154
BfmI CTRYAG 1 cut(s) 802
BfuAI ACCTGC 3 cut(s) 232, 362, 806
BisI GCNGC 7 cut(s) 216, 646, 935, 938, 1164, 1178, 1350
BlsI GCNGC 7 cut(s) 217, 647, 936, 939, 1165, 1179, 1351
BmcAI AGTACT 2 cut(s) 878, 1258
Bme1390I CCNGG 4 cut(s) 1022, 1129, 1138, 1418
Bme18I GGWCC 2 cut(s) 293, 1412
BmeT110I CYCGRG 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 293, 1297, 1412
BmiI GGNNCC 6 cut(s) 727, 1019, 1135, 1264, 1365, 1414
BmrFI CCNGG 4 cut(s) 1022, 1129, 1138, 1418
BmsI GCATC 4 cut(s) 1150, 1190, 1258, 1273
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 1 cut(s) 1043
BpuEI CTTGAG 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 1138
BsaJI CCNNGG 3 cut(s) 226, 1128, 1416
BsaWI WCCGGW 1 cut(s) 1366
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 589, 962, 1187
Bse118I RCCGGY 2 cut(s) 179, 454
Bse1I ACTGG 4 cut(s) 388, 874, 1254, 1264
Bse3DI GCAATG 2 cut(s) 920, 1172
BseBI CCWGG 3 cut(s) 1022, 1129, 1418
BseDI CCNNGG 3 cut(s) 226, 1128, 1416
BseGI GGATG 3 cut(s) 504, 1168, 1205
BseLI CCNNNNNNNGG 4 cut(s) 241, 589, 962, 1187
BseMI GCAATG 2 cut(s) 920, 1172
BseMII CTCAG 4 cut(s) 432, 536, 1068, 1292
BseNI ACTGG 4 cut(s) 388, 874, 1254, 1264
BseSI GKGCMC 1 cut(s) 1524
BseXI GCAGC 6 cut(s) 227, 632, 946, 1150, 1189, 1361
BseYI CCCAGC 1 cut(s) 662
BsgI GTGCAG 2 cut(s) 260, 906
Bsh1236I CGCG 1 cut(s) 1470
BshFI GGCC 2 cut(s) 923, 1298
BshNI GGYRCC 1 cut(s) 1363
BsiHKAI GWGCWC 1 cut(s) 950
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 4 cut(s) 180, 455, 1137, 1367
BslFI GGGAC 2 cut(s) 809, 1398
BslI CCNNNNNNNGG 4 cut(s) 241, 589, 962, 1187
BsmAI GTCTC 3 cut(s) 421, 674, 1107
BsmFI GGGAC 2 cut(s) 809, 1398
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 2 cut(s) 923, 1298
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 950, 1524
Bsp143I GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
BspACI CCGC 2 cut(s) 5, 938
BspANI GGCC 2 cut(s) 923, 1298
BspCNI CTCAG 4 cut(s) 431, 537, 1069, 1293
BspFNI CGCG 1 cut(s) 1470
BspLI GGNNCC 6 cut(s) 727, 1019, 1135, 1264, 1365, 1414
BspMI ACCTGC 3 cut(s) 232, 362, 806
BspPI GGATC 5 cut(s) 86, 541, 676, 863, 947
BspT107I GGYRCC 1 cut(s) 1363
BsrDI GCAATG 2 cut(s) 920, 1172
BsrFI RCCGGY 2 cut(s) 179, 454
BsrI ACTGG 4 cut(s) 388, 874, 1254, 1264
BssAI RCCGGY 2 cut(s) 179, 454
BssECI CCNNGG 3 cut(s) 226, 1128, 1416
BssMI GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
Bst2UI CCWGG 3 cut(s) 1022, 1129, 1418
Bst4CI ACNGT 5 cut(s) 68, 95, 679, 806, 1220
Bst6I CTCTTC 2 cut(s) 135, 1007
BstC8I GCNNGC 4 cut(s) 456, 1347, 1377, 1468
BstDEI CTNAG 6 cut(s) 23, 418, 517, 545, 1077, 1301
BstENI CCTNNNNNAGG 1 cut(s) 960
BstF5I GGATG 3 cut(s) 504, 1168, 1205
BstFNI CGCG 1 cut(s) 1470
BstHHI GCGC 2 cut(s) 753, 1472
BstKTI GATC 9 cut(s) 81, 127, 177, 445, 536, 684, 858, 955, 1246
BstMAI GTCTC 3 cut(s) 421, 674, 1107
BstMBI GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 3 cut(s) 1022, 1129, 1418
BstNSI RCATGY 1 cut(s) 1349
BstSCI CCNGG 4 cut(s) 1020, 1127, 1136, 1416
BstSFI CTRYAG 1 cut(s) 802
BstSLI GKGCMC 1 cut(s) 1524
BstUI CGCG 1 cut(s) 1470
BstV1I GCAGC 6 cut(s) 227, 632, 946, 1150, 1189, 1361
BstX2I RGATCY 2 cut(s) 533, 855
BstXI CCANNNNNNTGG 2 cut(s) 881, 1360
BstYI RGATCY 2 cut(s) 533, 855
BsuRI GGCC 2 cut(s) 923, 1298
BtgZI GCGATG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 290
BtsCI GGATG 3 cut(s) 504, 1168, 1205
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 6 cut(s) 100, 273, 330, 684, 811, 948
BveI ACCTGC 3 cut(s) 232, 362, 806
Cac8I GCNNGC 4 cut(s) 456, 1347, 1377, 1468
CfoI GCGC 2 cut(s) 753, 1472
Cfr10I RCCGGY 2 cut(s) 179, 454
Cfr13I GGNCC 3 cut(s) 293, 1297, 1412
Csp6I GTAC 3 cut(s) 877, 1257, 1384
CviAII CATG 7 cut(s) 30, 364, 902, 1084, 1117, 1204, 1346
CviQI GTAC 3 cut(s) 877, 1257, 1384
DdeI CTNAG 6 cut(s) 23, 418, 517, 545, 1077, 1301
DpnI GATC 9 cut(s) 80, 126, 176, 444, 535, 683, 857, 954, 1245
DpnII GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
Eam1104I CTCTTC 2 cut(s) 135, 1007
EarI CTCTTC 2 cut(s) 135, 1007
Eco32I GATATC 2 cut(s) 514, 1102
Eco47I GGWCC 2 cut(s) 293, 1412
Eco57I CTGAAG 2 cut(s) 981, 1457
Eco88I CYCGRG 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 960
EcoRII CCWGG 3 cut(s) 1020, 1127, 1416
EcoRV GATATC 2 cut(s) 514, 1102
EcoT22I ATGCAT 1 cut(s) 1205
FaeI CATG 7 cut(s) 33, 367, 905, 1087, 1120, 1207, 1349
FaqI GGGAC 2 cut(s) 809, 1398
FatI CATG 7 cut(s) 29, 363, 901, 1083, 1116, 1203, 1345
FauNDI CATATG 1 cut(s) 832
FbaI TGATCA 1 cut(s) 1243
Fnu4HI GCNGC 7 cut(s) 216, 646, 935, 938, 1164, 1178, 1350
FokI GGATG 3 cut(s) 511, 1155, 1212
Fsp4HI GCNGC 7 cut(s) 216, 646, 935, 938, 1164, 1178, 1350
FspBI CTAG 3 cut(s) 75, 200, 1154
GlaI GCGC 2 cut(s) 752, 1471
GluI GCNGC 7 cut(s) 216, 646, 935, 938, 1164, 1178, 1350
GsaI CCCAGC 1 cut(s) 666
GsuI CTGGAG 1 cut(s) 1043
HaeIII GGCC 2 cut(s) 923, 1298
HapII CCGG 4 cut(s) 180, 455, 1137, 1367
HhaI GCGC 2 cut(s) 753, 1472
Hin1II CATG 7 cut(s) 33, 367, 905, 1087, 1120, 1207, 1349
Hin6I GCGC 2 cut(s) 751, 1470
HinP1I GCGC 2 cut(s) 751, 1470
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 4 cut(s) 180, 455, 1137, 1367
HphI GGTGA 3 cut(s) 434, 722, 1169
Hpy166II GTNNAC 4 cut(s) 293, 432, 568, 809
Hpy188III TCNNGA 5 cut(s) 509, 578, 959, 1046, 1067
Hpy8I GTNNAC 4 cut(s) 293, 432, 568, 809
HpyAV CCTTC 3 cut(s) 139, 934, 956
HpyCH4III ACNGT 5 cut(s) 68, 95, 679, 806, 1220
HpyCH4IV ACGT 2 cut(s) 289, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 6 cut(s) 23, 418, 517, 545, 1077, 1301
HpySE526I ACGT 2 cut(s) 289, 348
Hsp92II CATG 7 cut(s) 33, 367, 905, 1087, 1120, 1207, 1349
HspAI GCGC 2 cut(s) 751, 1470
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 1243
Kzo9I GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
LmnI GCTCC 5 cut(s) 502, 725, 866, 1023, 1268
Lsp1109I GCAGC 6 cut(s) 227, 632, 946, 1150, 1189, 1361
LweI GCATC 4 cut(s) 1150, 1190, 1258, 1273
MaeI CTAG 3 cut(s) 75, 200, 1154
MaeII ACGT 2 cut(s) 289, 348
MaeIII GTNAC 2 cut(s) 673, 1220
MalI GATC 9 cut(s) 80, 126, 176, 444, 535, 683, 857, 954, 1245
MboI GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
MboII GAAGA 7 cut(s) 152, 701, 850, 1024, 1096, 1317, 1450
MfeI CAATTG 1 cut(s) 1287
MflI RGATCY 2 cut(s) 533, 855
MhlI GDGCHC 2 cut(s) 950, 1524
MluCI AATT 9 cut(s) 168, 297, 758, 790, 1062, 1287, 1407, 1481, 1496
MlyI GAGTC 6 cut(s) 49, 251, 423, 676, 804, 1088
MmeI TCCRAC 1 cut(s) 511
Mph1103I ATGCAT 1 cut(s) 1205
MroNI GCCGGC 1 cut(s) 454
MseI TTAA 3 cut(s) 165, 651, 837
MslI CAYNNNNRTG 2 cut(s) 628, 1358
MspI CCGG 4 cut(s) 180, 455, 1137, 1367
MspR9I CCNGG 4 cut(s) 1022, 1129, 1138, 1418
MunI CAATTG 1 cut(s) 1287
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 3 cut(s) 1022, 1129, 1418
MvnI CGCG 1 cut(s) 1470
MwoI GCNNNNNNNGC 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 456
NciI CCSGG 1 cut(s) 1138
NdeI CATATG 1 cut(s) 832
NdeII GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 7 cut(s) 33, 367, 905, 1087, 1120, 1207, 1349
NlaIV GGNNCC 6 cut(s) 727, 1019, 1135, 1264, 1365, 1414
NsiI ATGCAT 1 cut(s) 1205
NspI RCATGY 1 cut(s) 1349
PaeI GCATGC 1 cut(s) 1349
PaeR7I CTCGAG 1 cut(s) 259
PaqCI CACCTGC 2 cut(s) 232, 806
PctI GAATGC 1 cut(s) 601
PdiI GCCGGC 1 cut(s) 456
PfeI GAWTC 4 cut(s) 231, 393, 607, 735
PflFI GACNNNGTC 1 cut(s) 413
PflMI CCANNNNNTGG 1 cut(s) 1187
PfoI TCCNGGA 1 cut(s) 1020
PkrI GCNGC 7 cut(s) 217, 647, 936, 939, 1165, 1179, 1351
PleI GAGTC 6 cut(s) 48, 251, 422, 675, 804, 1087
PpsI GAGTC 6 cut(s) 48, 251, 422, 675, 804, 1087
Psp6I CCWGG 3 cut(s) 1020, 1127, 1416
PspFI CCCAGC 1 cut(s) 662
PspGI CCWGG 3 cut(s) 1020, 1127, 1416
PspN4I GGNNCC 6 cut(s) 727, 1019, 1135, 1264, 1365, 1414
PspPI GGNCC 3 cut(s) 293, 1297, 1412
PspXI VCTCGAGB 1 cut(s) 259
PsuI RGATCY 2 cut(s) 533, 855
PsyI GACNNNGTC 1 cut(s) 413
RsaI GTAC 3 cut(s) 878, 1258, 1385
RsaNI GTAC 3 cut(s) 877, 1257, 1384
RseI CAYNNNNRTG 2 cut(s) 628, 1358
SaqAI TTAA 3 cut(s) 165, 651, 837
SatI GCNGC 7 cut(s) 216, 646, 935, 938, 1164, 1178, 1350
Sau3AI GATC 9 cut(s) 78, 124, 174, 442, 533, 681, 855, 952, 1243
Sau96I GGNCC 3 cut(s) 293, 1297, 1412
ScaI AGTACT 2 cut(s) 878, 1258
SchI GAGTC 6 cut(s) 49, 251, 423, 676, 804, 1088
ScrFI CCNGG 4 cut(s) 1022, 1129, 1138, 1418
SduI GDGCHC 2 cut(s) 950, 1524
SfaNI GCATC 4 cut(s) 1150, 1190, 1258, 1273
SfcI CTRYAG 1 cut(s) 802
Sfr274I CTCGAG 1 cut(s) 259
SgrAI CRCCGGYG 1 cut(s) 179
SinI GGWCC 2 cut(s) 293, 1412
SlaI CTCGAG 1 cut(s) 259
SmiMI CAYNNNNRTG 2 cut(s) 628, 1358
SmlI CTYRAG 2 cut(s) 259, 483
SmoI CTYRAG 2 cut(s) 259, 483
SphI GCATGC 1 cut(s) 1349
Sse9I AATT 9 cut(s) 168, 297, 758, 790, 1062, 1287, 1407, 1481, 1496
SsiI CCGC 2 cut(s) 5, 938
SspMI CTAG 3 cut(s) 75, 200, 1154
StyD4I CCNGG 4 cut(s) 1020, 1127, 1136, 1416
TaaI ACNGT 5 cut(s) 68, 95, 679, 806, 1220
TaiI ACGT 2 cut(s) 292, 351
TaqI TCGA 5 cut(s) 260, 605, 755, 1107, 1357
TasI AATT 9 cut(s) 168, 297, 758, 790, 1062, 1287, 1407, 1481, 1496
TatI WGTACW 2 cut(s) 876, 1256
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 4 cut(s) 231, 393, 607, 735
Tru1I TTAA 3 cut(s) 165, 651, 837
Tru9I TTAA 3 cut(s) 165, 651, 837
TscAI CASTG 6 cut(s) 100, 273, 330, 684, 811, 955
TseI GCWGC 6 cut(s) 215, 645, 934, 1163, 1177, 1349
TspDTI ATGAA 2 cut(s) 18, 562
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 6 cut(s) 100, 273, 330, 684, 811, 955
Tth111I GACNNNGTC 1 cut(s) 413
Van91I CCANNNNNTGG 1 cut(s) 1187
VpaK11BI GGWCC 2 cut(s) 293, 1412
XagI CCTNNNNNAGG 1 cut(s) 960
XapI RAATTY 2 cut(s) 168, 1496
XceI RCATGY 1 cut(s) 1349
XhoI CTCGAG 1 cut(s) 259
XspI CTAG 3 cut(s) 75, 200, 1154
ZrmI AGTACT 2 cut(s) 878, 1258
Zsp2I ATGCAT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.