pycom07g00300

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0017386
Physical Location & Seq
Reverse (-)
298416 .. 300218
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00300.1

Sequence Viewer

Length: 981 bp
ATGGAGCTTCTGGATATCTTAGTCAATGCAGTTGGAAATGACATTCTGAGAAACAAATCGAAAGTGGTGGACTTCATTCAAGACCCTAACAAGGTTGAGTGGCATTCGATTCAACTAATCCTAACATATTGGTGCATTGGTCTTTGCTGCCTTAATGCTTTTGTTACAGTGATCCTTGAAGATGGACAACGATATGATGGTGATGTTTTAGTAGGAGCCGATGGAATCTGGTCAAAGGTTCGCTCGAAATTGTTTGGCATACGGGAAGCAAAATACTCAAATTACACCTGCTACAGTGGACTCACTAAAGTTGTCCCATCATATGTTAATAGTGTTGGGTATCGGATCTTCTTGGGAACGAACCAGTACTTTGGTGCAGTAGAAGTTGGGCATGGGAATATGCAATGGTTGGCCTTCCATAAGCAGCCACCAATGAGCACTGATCCTCCTGAAGGTAAAAAGAAGAGGCTCCTGGAGGTATTTGGAAAATGGTGTCAGGAAGTGGTTGCTCTAATTCAAGAAACACCTGAGTCCATGATTTTACAAAGAGATATCTACGATAGAGACATGATATATTCCTGGGGAACCGGGCGTGTTGCTCTGCTAGGTGATGCTGCTCATCCATTGCAGCCAAATCTTGGAATAGGAGGATGCATGGCAATTGAGGACAGTTACCAGCTTATAGATGAGGTTGATCAAGTTTCCAGTACTGGCTCCAATGCTCAGAGGACAGATGCAATTGTCTTGGCCCTGAGAAGATATGCAACAAAAAGAATGAGGCGTGTTGGTATAGTGCATGCAGCCACCCGAATGGCACCGGAAATGCTTGCTATGTACCGACCTTGCATAGAGTTCAAATTTGGTCCCCTGGCTCATCTATCAACTCTGAAGATAATGCACCCTGCATTTCCGATGGCTCGTGCATTTCTGCAATTTTGTATGCCAGAATTTATGACTTGGATAATAACAGGGCATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

327

Amino Acids

36.75

Weight (kDa)

7.59

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 64 - 234 4e-08 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 638
AclWI GGATC 3 cut(s) 166, 353, 437
AcsI RAATTY 2 cut(s) 857, 947
AcuI CTGAAG 2 cut(s) 471, 908
AfaI GTAC 3 cut(s) 368, 709, 836
AfiI CCNNNNNNNGG 3 cut(s) 91, 452, 638
AgsI TTSAA 5 cut(s) 80, 113, 179, 518, 856
AjnI CCWGG 3 cut(s) 471, 578, 867
AluBI AGCT 2 cut(s) 7, 679
AluI AGCT 2 cut(s) 7, 679
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 3 cut(s) 166, 353, 437
AoxI GGCC 2 cut(s) 411, 747
ApeKI GCWGC 5 cut(s) 147, 424, 614, 628, 800
ApoI RAATTY 2 cut(s) 857, 947
AspS9I GGNCC 2 cut(s) 748, 863
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 2 cut(s) 212, 620
AvaII GGWCC 1 cut(s) 863
BanI GGYRCC 1 cut(s) 814
BarI GAAGNNNNNNTAC 2 cut(s) 332, 364
BauI CACGAG 1 cut(s) 918
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 5 cut(s) 134, 436, 601, 640, 812
BccI CCATC 5 cut(s) 176, 191, 215, 325, 907
BciT130I CCWGG 3 cut(s) 473, 580, 869
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 296
BisI GCNGC 5 cut(s) 148, 425, 615, 629, 801
BlsI GCNGC 5 cut(s) 149, 426, 616, 630, 802
BmcAI AGTACT 2 cut(s) 368, 709
Bme1390I CCNGG 4 cut(s) 473, 580, 589, 869
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 748, 863
BmiI GGNNCC 6 cut(s) 217, 470, 586, 715, 816, 865
BmrFI CCNGG 4 cut(s) 473, 580, 589, 869
BmsI GCATC 3 cut(s) 601, 641, 724
BpmI CTGGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 589
BsaJI CCNNGG 2 cut(s) 579, 867
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 452, 638
Bse1I ACTGG 3 cut(s) 364, 705, 715
Bse3DI GCAATG 2 cut(s) 410, 623
BseBI CCWGG 3 cut(s) 473, 580, 869
BseDI CCNNGG 2 cut(s) 579, 867
BseGI GGATG 2 cut(s) 619, 656
BseLI CCNNNNNNNGG 3 cut(s) 91, 452, 638
BseMI GCAATG 2 cut(s) 410, 623
BseMII CTCAG 4 cut(s) 38, 519, 737, 743
BseNI ACTGG 3 cut(s) 364, 705, 715
BseXI GCAGC 5 cut(s) 134, 436, 601, 640, 812
BsgI GTGCAG 1 cut(s) 396
BshFI GGCC 2 cut(s) 413, 749
BshNI GGYRCC 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 2 cut(s) 588, 818
BslFI GGGAC 2 cut(s) 299, 849
BslI CCNNNNNNNGG 3 cut(s) 91, 452, 638
BsmAI GTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 299, 849
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 2 cut(s) 413, 749
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 4 cut(s) 171, 345, 442, 694
BspANI GGCC 2 cut(s) 413, 749
BspCNI CTCAG 4 cut(s) 39, 520, 736, 744
BspLI GGNNCC 6 cut(s) 217, 470, 586, 715, 816, 865
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 3 cut(s) 166, 353, 437
BspT107I GGYRCC 1 cut(s) 814
BsrDI GCAATG 2 cut(s) 410, 623
BsrI ACTGG 3 cut(s) 364, 705, 715
BssECI CCNNGG 2 cut(s) 579, 867
BssMI GATC 4 cut(s) 171, 345, 442, 694
BssSI CACGAG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 3 cut(s) 473, 580, 869
Bst4CI ACNGT 3 cut(s) 169, 296, 671
Bst6I CTCTTC 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 798, 828
BstDEI CTNAG 5 cut(s) 19, 47, 528, 723, 752
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 2 cut(s) 619, 656
BstKTI GATC 4 cut(s) 174, 348, 445, 697
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 4 cut(s) 171, 345, 442, 694
BstNI CCWGG 3 cut(s) 473, 580, 869
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 4 cut(s) 471, 578, 587, 867
BstSFI CTRYAG 1 cut(s) 292
BstV1I GCAGC 5 cut(s) 134, 436, 601, 640, 812
BstX2I RGATCY 1 cut(s) 345
BstXI CCANNNNNNTGG 2 cut(s) 371, 811
BstYI RGATCY 1 cut(s) 345
BsuRI GGCC 2 cut(s) 413, 749
BtsCI GGATG 2 cut(s) 619, 656
BtsIMutI CAGTG 3 cut(s) 174, 301, 438
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 798, 828
Cfr13I GGNCC 2 cut(s) 748, 863
Csp6I GTAC 3 cut(s) 367, 708, 835
CviAII CATG 6 cut(s) 392, 535, 568, 655, 797, 974
CviQI GTAC 3 cut(s) 367, 708, 835
DdeI CTNAG 5 cut(s) 19, 47, 528, 723, 752
DpnI GATC 4 cut(s) 173, 347, 444, 696
DpnII GATC 4 cut(s) 171, 345, 442, 694
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
Eco32I GATATC 2 cut(s) 16, 553
Eco47I GGWCC 1 cut(s) 863
Eco57I CTGAAG 2 cut(s) 471, 908
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoRII CCWGG 3 cut(s) 471, 578, 867
EcoRV GATATC 2 cut(s) 16, 553
EcoT22I ATGCAT 1 cut(s) 656
FaeI CATG 6 cut(s) 395, 538, 571, 658, 800, 977
FaqI GGGAC 2 cut(s) 299, 849
FatI CATG 6 cut(s) 391, 534, 567, 654, 796, 973
FauNDI CATATG 1 cut(s) 322
FbaI TGATCA 1 cut(s) 694
Fnu4HI GCNGC 5 cut(s) 148, 425, 615, 629, 801
FokI GGATG 2 cut(s) 606, 663
Fsp4HI GCNGC 5 cut(s) 148, 425, 615, 629, 801
FspBI CTAG 1 cut(s) 605
GluI GCNGC 5 cut(s) 148, 425, 615, 629, 801
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 2 cut(s) 413, 749
HapII CCGG 2 cut(s) 588, 818
Hin1II CATG 6 cut(s) 395, 538, 571, 658, 800, 977
HinfI GANTC 4 cut(s) 109, 225, 300, 530
HpaII CCGG 2 cut(s) 588, 818
HphI GGTGA 2 cut(s) 212, 620
Hpy166II GTNNAC 2 cut(s) 70, 299
Hpy188I TCNGA 5 cut(s) 48, 345, 726, 888, 912
Hpy188III TCNNGA 5 cut(s) 11, 80, 449, 497, 518
Hpy8I GTNNAC 2 cut(s) 70, 299
HpyAV CCTTC 2 cut(s) 424, 446
HpyCH4III ACNGT 3 cut(s) 169, 296, 671
HpyF3I CTNAG 5 cut(s) 19, 47, 528, 723, 752
Hsp92II CATG 6 cut(s) 395, 538, 571, 658, 800, 977
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 4 cut(s) 171, 345, 442, 694
LmnI GCTCC 4 cut(s) 4, 215, 474, 719
Lsp1109I GCAGC 5 cut(s) 134, 436, 601, 640, 812
LweI GCATC 3 cut(s) 601, 641, 724
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 163, 671
MalI GATC 4 cut(s) 173, 347, 444, 696
MboI GATC 4 cut(s) 171, 345, 442, 694
MboII GAAGA 5 cut(s) 191, 340, 475, 768, 901
MfeI CAATTG 2 cut(s) 660, 738
MflI RGATCY 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 8 cut(s) 248, 280, 513, 660, 738, 857, 932, 947
MlyI GAGTC 2 cut(s) 294, 539
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 8 cut(s) 456, 459, 469, 641, 658, 682, 720, 771
Mph1103I ATGCAT 1 cut(s) 656
MseI TTAA 2 cut(s) 153, 327
MslI CAYNNNNRTG 2 cut(s) 130, 809
MspI CCGG 2 cut(s) 588, 818
MspR9I CCNGG 4 cut(s) 473, 580, 589, 869
MunI CAATTG 2 cut(s) 660, 738
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 3 cut(s) 473, 580, 869
NciI CCSGG 1 cut(s) 589
NdeI CATATG 1 cut(s) 322
NdeII GATC 4 cut(s) 171, 345, 442, 694
NlaIII CATG 6 cut(s) 395, 538, 571, 658, 800, 977
NlaIV GGNNCC 6 cut(s) 217, 470, 586, 715, 816, 865
NsiI ATGCAT 1 cut(s) 656
NspI RCATGY 1 cut(s) 800
PaeI GCATGC 1 cut(s) 800
PaqCI CACCTGC 1 cut(s) 296
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 2 cut(s) 109, 225
PflMI CCANNNNNTGG 1 cut(s) 638
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 5 cut(s) 149, 426, 616, 630, 802
PleI GAGTC 2 cut(s) 294, 538
PpsI GAGTC 2 cut(s) 294, 538
Psp6I CCWGG 3 cut(s) 471, 578, 867
PspGI CCWGG 3 cut(s) 471, 578, 867
PspN4I GGNNCC 6 cut(s) 217, 470, 586, 715, 816, 865
PspPI GGNCC 2 cut(s) 748, 863
PsuI RGATCY 1 cut(s) 345
RsaI GTAC 3 cut(s) 368, 709, 836
RsaNI GTAC 3 cut(s) 367, 708, 835
RseI CAYNNNNRTG 2 cut(s) 130, 809
SaqAI TTAA 2 cut(s) 153, 327
SatI GCNGC 5 cut(s) 148, 425, 615, 629, 801
Sau3AI GATC 4 cut(s) 171, 345, 442, 694
Sau96I GGNCC 2 cut(s) 748, 863
ScaI AGTACT 2 cut(s) 368, 709
SchI GAGTC 2 cut(s) 294, 539
ScrFI CCNGG 4 cut(s) 473, 580, 589, 869
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 3 cut(s) 601, 641, 724
SfcI CTRYAG 1 cut(s) 292
SinI GGWCC 1 cut(s) 863
SmiMI CAYNNNNRTG 2 cut(s) 130, 809
SphI GCATGC 1 cut(s) 800
Sse9I AATT 8 cut(s) 248, 280, 513, 660, 738, 857, 932, 947
SspMI CTAG 1 cut(s) 605
StyD4I CCNGG 4 cut(s) 471, 578, 587, 867
TaaI ACNGT 3 cut(s) 169, 296, 671
TaqI TCGA 3 cut(s) 59, 107, 245
TasI AATT 8 cut(s) 248, 280, 513, 660, 738, 857, 932, 947
TatI WGTACW 2 cut(s) 366, 707
TfiI GAWTC 2 cut(s) 109, 225
Tru1I TTAA 2 cut(s) 153, 327
Tru9I TTAA 2 cut(s) 153, 327
TscAI CASTG 3 cut(s) 174, 301, 445
TseI GCWGC 5 cut(s) 147, 424, 614, 628, 800
TspDTI ATGAA 1 cut(s) 64
TspRI CASTG 3 cut(s) 174, 301, 445
Van91I CCANNNNNTGG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 863
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 2 cut(s) 857, 947
XceI RCATGY 1 cut(s) 800
XspI CTAG 1 cut(s) 605
ZrmI AGTACT 2 cut(s) 368, 709
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.