Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0016936

Physical Location & Seq

Genomic Coordinates Reverse (-)

37415988 .. 37417652

Gene Structure 1665 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1319900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGTTGTCACAGCTGTGGTCGTTGCTGGGGCTGCTGACAGTCCTCCAGAATGTGCTGCCTTCCCAGCTGCTCTCCCTCCTCCACTCCCTATACGAGTCCCTCCAAGACATGCTCACCCCCTACTCCTACTTCGACGTCCCCGAGTTCAACGGCTACTGCGGCGTCCACCTCAATCACCTCTACCGCCACGTCAACCTCTACCTCAACTCCCTCGTCAACGCCAACACCTCTGCCAGTTCTGCCACCTCCTCCTCCTCCTCATTCAATTCCCGCCTCACCCTCTCCCGCTCCAACTCTTCCAACCGCATCTCCTTCACCGTCGCCCCCAACCACTCCGTCCACGACTCCTTCCGCGGCCTCACCCTCTCCTGGACCCACCACGTCGACACAGTCCAAGACTCCCTTGAAGAGAAGCGCTCCTTCGTCCTCAAGCTCCCCAAGCGCCACCGCCACGCCCTCCTCCAGCCTTACCTCCACCATCTCACCGCACGTGCCGAGGAATTCGAGCGCGTCTCTCGTGAGAGGCGGCTCTTCACCAACAACGGCCACGCCTCCTACGACTCCGGCTGGGTCTCCGTCCCCTTCCGTCACCCCTCCACCTTCGAAACCCTCGCTCTTGAACCCCAACTCAAGAACCAGCTTACCGGAGACCTCACGGCGTTTGCCGAGGGCAAAGAGTTTTACCACAGGGTGGGACGCGCGTGGAAGCGGGGTTACCTTCTGTACGGCCCTCCCGGTTCAGGCAAGTCGAGCTTGATCGCTGCCATGGCCAACTACCTGTGCTACGACGTCTACGACCTCGAGCTCACCAAGGTTTCTGACAACTCGGAGCTGCGAGCTCTGCTCATACAGACCACCAACCGCTCCATCGTAGTCATAGAAGACATAGACTGCTCCCTCGATCTGACGGCTGACAGGCAGCAGAAAATGATCTCCAATTCAACTTCCAAGGCTAGACTGAGATCATCGAGGATGCAGGCTCAGCAGAAGCAGGACAACGACGAGCAAAACGGCGGAGGGAGAGTGACGCTGTCGGGGTTGCTCAACTTCACGGACGGCCTCTGGTCTTGTTGTGGAGATGAACGCATCATAGTGTTCACCACCAACCACAGGGACAATGTGGACCCGGCGCTGCTGCGCTGCGGACGCATGGACGTCCACGTCAGCCTCGGCACCTGCGGTCCCCTCGCCTTCCGGCAGCTGGCAAAGAACTACTTGGGGCTCGATGCCCACCCTCTCTTCGAAGCAGCGGACGGGTGCGTGAGGTCGGGCGGGGTACTCACGCCGGCCCAGGTAGGAGAGATTCTGTTAAGGAACAGAGGGAATGCTGACGTGGCAATGAAGGAGGTGGTGTTGGCGATGCAGGCCAGAATCAACTTGGGTGTTGGTGGGGGAAGCCGAGATCAACTAGCGGAGCTTTACTCATCGTGCGATGATCAGACGGTGAGGATGAGGTCATCGTCACCGGAGAGCGTGCTGGTGGGAGTGGGTGGTGGGTCACCGGAAAATTGGGTGACGTCGTCACCGCGGGGGATGGGGAGGAAGAAGAAGGCGGCTGCCGGGGCTGAAGCTGAAGGGGATCAGAGCAGCCAGAGGATGAAGATGAAATTGTTAGTGAGGCTTAGGTCTTTGACGAAGTCCGACTCCGGAAGGAGGGGAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc Pkinase

Protein Analysis

555

Amino Acids

61.32

Weight (kDa)

8.94

Isoelectric Point (pI)

47.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	19 - 127	1e-15	Domain associated at C-terminal with AAA
AAA	PF00004	239 - 390	1.1e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	393 - 455	1.1e-10	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016936)

Species	Orthologous Gene IDs
malus_domestica	MD02G1319900.v1.1
prunus_persica	Prupe.2G002200_v2.0.a1
pyrus_communis	pycom02g26790
rosa_chinensis	RchiOBHm_Chr1g0313201
rosa_laevigata	RLG00000030816
rosa_multiflora	Rmu_sc0031618.1_g000001 Rmu_sc0031619.1_g000001
rosa_roxburghii	Rroxscaffold_4G00332390
rosa_rugosa	RorugPtG0001400.1
rosa_samantha	Rh1AG001600 Rh1CG003600 Rh1CG006200 Rh1DG001500
rosa_wichuraiana	Rw1G000070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1184
AasI	GACNNNNNNGTC	1 cut(s)	1160
AatII	GACGTC	4 cut(s)	138, 792, 1158, 1520
Acc36I	ACCTGC	1 cut(s)	1184
AccB1I	GGYRCC	1 cut(s)	1172
AccB7I	CCANNNNNTGG	1 cut(s)	691
AccBSI	CCGCTC	2 cut(s)	288, 864
AccI	GTMKAC	2 cut(s)	384, 792
AccII	CGCG	5 cut(s)	354, 510, 699, 701, 1528
AccIII	TCCGGA	1 cut(s)	1646
AclWI	GGATC	1 cut(s)	1587
AcoI	YGGCCR	2 cut(s)	544, 768
AcsI	RAATTY	1 cut(s)	500
AcuI	CTGAAG	2 cut(s)	1587, 1593
AcvI	CACGTG	1 cut(s)	491
AcyI	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
AdeI	CACNNNGTG	1 cut(s)	691
AfaI	GTAC	2 cut(s)	725, 1278
AfeI	AGCGCT	1 cut(s)	416
AfiI	CCNNNNNNNGG	6 cut(s)	369, 691, 740, 1110, 1201, 1653
AgsI	TTSAA	5 cut(s)	148, 265, 407, 620, 942
AjiI	CACGTC	4 cut(s)	190, 382, 1162, 1333
AjnI	CCWGG	2 cut(s)	368, 1290
AleI	CACNNNNGTG	1 cut(s)	13
Alw21I	GWGCWC	2 cut(s)	807, 841
Alw26I	GTCTC	3 cut(s)	517, 577, 642
AlwI	GGATC	1 cut(s)	1587
Ama87I	CYCGRG	2 cut(s)	140, 800
Aor13HI	TCCGGA	1 cut(s)	1646
Aor51HI	AGCGCT	1 cut(s)	416
AoxI	GGCC	7 cut(s)	355, 544, 727, 768, 1057, 1287, 1365
ApoI	RAATTY	1 cut(s)	500
AspLEI	GCGC	6 cut(s)	417, 444, 510, 701, 1132, 1140
AspS9I	GGNCC	5 cut(s)	372, 728, 1123, 1181, 1288
AsuC2I	CCSGG	3 cut(s)	735, 1127, 1561
AsuII	TTCGAA	2 cut(s)	603, 1242
AvaI	CYCGRG	2 cut(s)	140, 800
AvaII	GGWCC	3 cut(s)	372, 1123, 1181
BalI	TGGCCA	1 cut(s)	770
BanI	GGYRCC	1 cut(s)	1172
BanII	GRGCYC	3 cut(s)	807, 841, 1224
BarI	GAAGNNNNNNTAC	2 cut(s)	698, 730
BauI	CACGAG	1 cut(s)	516
BbrPI	CACGTG	1 cut(s)	491
BbsI	GAAGAC	1 cut(s)	888
Bbv12I	GWGCWC	2 cut(s)	807, 841
BccI	CCATC	3 cut(s)	486, 875, 1528
BceAI	ACGGC	7 cut(s)	166, 559, 672, 742, 924, 1027, 1072
BciT130I	CCWGG	2 cut(s)	370, 1292
BclI	TGATCA	1 cut(s)	1435
BcnI	CCSGG	3 cut(s)	735, 1127, 1561
BcoDI	GTCTC	3 cut(s)	517, 577, 642
BfaI	CTAG	2 cut(s)	954, 1409
BfoI	RGCGCY	3 cut(s)	418, 445, 1133
BfuAI	ACCTGC	1 cut(s)	1184
BlpI	GCTNAGC	1 cut(s)	981
Bme1390I	CCNGG	5 cut(s)	370, 735, 1127, 1292, 1561
Bme18I	GGWCC	3 cut(s)	372, 1123, 1181
BmeT110I	CYCGRG	2 cut(s)	140, 800
BmgBI	CACGTC	4 cut(s)	190, 382, 1162, 1333
BmgT120I	GGNCC	5 cut(s)	372, 728, 1123, 1181, 1288
BmiI	GGNNCC	4 cut(s)	374, 1125, 1174, 1183
BmrFI	CCNGG	5 cut(s)	370, 735, 1127, 1292, 1561
BmsI	GCATC	5 cut(s)	315, 963, 1095, 1216, 1350
BpiI	GAAGAC	1 cut(s)	888
BplI	GAGNNNNNCTC	8 cut(s)	401, 433, 497, 529, 828, 860, 1406, 1438
BpmI	CTGGAG	2 cut(s)	29, 446
Bpu10I	CCTNAGC	1 cut(s)	1622
Bpu1102I	GCTNAGC	1 cut(s)	981
Bpu14I	TTCGAA	2 cut(s)	603, 1242
BpuEI	CTTGAG	2 cut(s)	413, 614
BpuMI	CCSGG	3 cut(s)	735, 1127, 1561
BsaAI	YACGTR	1 cut(s)	491
BsaHI	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
BsaI	GGTCTC	2 cut(s)	577, 642
BsaWI	WCCGGW	4 cut(s)	644, 1465, 1501, 1646
BsaXI	ACNNNNNCTCC	8 cut(s)	191, 221, 293, 323, 1008, 1038, 1337, 1367
Bsc4I	CCNNNNNNNGG	6 cut(s)	369, 691, 740, 1110, 1201, 1653
Bse118I	RCCGGY	1 cut(s)	1285
Bse1I	ACTGG	1 cut(s)	234
Bse3DI	GCAATG	1 cut(s)	1344
BseAI	TCCGGA	1 cut(s)	1646
BseBI	CCWGG	2 cut(s)	370, 1292
BseGI	GGATG	4 cut(s)	978, 1455, 1539, 1602
BseLI	CCNNNNNNNGG	6 cut(s)	369, 691, 740, 1110, 1201, 1653
BseMI	GCAATG	1 cut(s)	1344
BseMII	CTCAG	2 cut(s)	950, 995
BseNI	ACTGG	1 cut(s)	234
BseRI	GAGGAG	6 cut(s)	68, 238, 241, 244, 247, 449
BseYI	CCCAGC	3 cut(s)	25, 63, 567
Bsh1236I	CGCG	5 cut(s)	354, 510, 699, 701, 1528
BshFI	GGCC	7 cut(s)	357, 546, 729, 770, 1059, 1289, 1367
BshNI	GGYRCC	1 cut(s)	1172
BsiHKAI	GWGCWC	2 cut(s)	807, 841
BsiHKCI	CYCGRG	2 cut(s)	140, 800
BslFI	GGGAC	6 cut(s)	82, 122, 563, 708, 1127, 1167
BslI	CCNNNNNNNGG	6 cut(s)	369, 691, 740, 1110, 1201, 1653
BsmAI	GTCTC	3 cut(s)	517, 577, 642
BsmBI	CGTCTC	1 cut(s)	517
BsmFI	GGGAC	6 cut(s)	82, 122, 563, 708, 1127, 1167
BsmI	GAATGC	1 cut(s)	1330
BsnI	GGCC	7 cut(s)	357, 546, 729, 770, 1059, 1289, 1367
Bso31I	GGTCTC	2 cut(s)	577, 642
BsoBI	CYCGRG	2 cut(s)	140, 800
Bsp119I	TTCGAA	2 cut(s)	603, 1242
Bsp1286I	GDGCHC	3 cut(s)	807, 841, 1224
Bsp13I	TCCGGA	1 cut(s)	1646
Bsp143I	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
Bsp1720I	GCTNAGC	1 cut(s)	981
Bsp19I	CCATGG	1 cut(s)	765
BspANI	GGCC	7 cut(s)	357, 546, 729, 770, 1059, 1289, 1367
BspCNI	CTCAG	2 cut(s)	951, 994
BspEI	TCCGGA	1 cut(s)	1646
BspFNI	CGCG	5 cut(s)	354, 510, 699, 701, 1528
BspLI	GGNNCC	4 cut(s)	374, 1125, 1174, 1183
BspMI	ACCTGC	1 cut(s)	1184
BspPI	GGATC	1 cut(s)	1587
BspQI	GCTCTTC	1 cut(s)	536
BspT104I	TTCGAA	2 cut(s)	603, 1242
BspT107I	GGYRCC	1 cut(s)	1172
BspTNI	GGTCTC	2 cut(s)	577, 642
BsrBI	CCGCTC	2 cut(s)	288, 864
BsrDI	GCAATG	1 cut(s)	1344
BsrFI	RCCGGY	1 cut(s)	1285
BsrI	ACTGG	1 cut(s)	234
BssAI	RCCGGY	1 cut(s)	1285
BssMI	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
BssNI	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
BssSI	CACGAG	1 cut(s)	516
BssT1I	CCWWGG	3 cut(s)	765, 810, 948
Bst2BI	CACGAG	1 cut(s)	516
Bst2UI	CCWGG	2 cut(s)	370, 1292
Bst4CI	ACNGT	4 cut(s)	40, 319, 391, 1444
Bst6I	CTCTTC	4 cut(s)	301, 402, 536, 1244
BstACI	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
BstBAI	YACGTR	1 cut(s)	491
BstBI	TTCGAA	2 cut(s)	603, 1242
BstC8I	GCNNGC	6 cut(s)	837, 978, 1203, 1287, 1365, 1475
BstDEI	CTNAG	3 cut(s)	959, 981, 1622
BstDSI	CCRYGG	3 cut(s)	352, 765, 1526
BstEII	GGTNACC	2 cut(s)	713, 1497
BstF5I	GGATG	4 cut(s)	978, 1455, 1539, 1602
BstFNI	CGCG	5 cut(s)	354, 510, 699, 701, 1528
BstH2I	RGCGCY	3 cut(s)	418, 445, 1133
BstHHI	GCGC	6 cut(s)	417, 444, 510, 701, 1132, 1140
BstKTI	GATC	7 cut(s)	759, 904, 933, 965, 1405, 1438, 1582
BstMAI	GTCTC	3 cut(s)	517, 577, 642
BstMBI	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
BstNI	CCWGG	2 cut(s)	370, 1292
BstNSI	RCATGY	1 cut(s)	112
BstPI	GGTNACC	2 cut(s)	713, 1497
BstSCI	CCNGG	5 cut(s)	368, 733, 1125, 1290, 1559
BstUI	CGCG	5 cut(s)	354, 510, 699, 701, 1528
BstV2I	GAAGAC	1 cut(s)	888
BsuRI	GGCC	7 cut(s)	357, 546, 729, 770, 1059, 1289, 1367
BtgI	CCRYGG	3 cut(s)	352, 765, 1526
BtgZI	GCGATG	2 cut(s)	1373, 1446
BtrI	CACGTC	4 cut(s)	190, 382, 1162, 1333
BtsCI	GGATG	4 cut(s)	978, 1455, 1539, 1602
BveI	ACCTGC	1 cut(s)	1184
Cac8I	GCNNGC	6 cut(s)	837, 978, 1203, 1287, 1365, 1475
CfoI	GCGC	6 cut(s)	417, 444, 510, 701, 1132, 1140
Cfr10I	RCCGGY	1 cut(s)	1285
Cfr13I	GGNCC	5 cut(s)	372, 728, 1123, 1181, 1288
Cfr42I	CCGCGG	2 cut(s)	355, 1529
CseI	GACGC	5 cut(s)	151, 499, 705, 1036, 1155
Csp6I	GTAC	2 cut(s)	724, 1277
CviAII	CATG	3 cut(s)	109, 766, 1150
CviQI	GTAC	2 cut(s)	724, 1277
DdeI	CTNAG	3 cut(s)	959, 981, 1622
DpnI	GATC	7 cut(s)	758, 903, 932, 964, 1404, 1437, 1581
DpnII	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
DraIII	CACNNNGTG	1 cut(s)	691
DrdI	GACNNNNNNGTC	1 cut(s)	1160
DseDI	GACNNNNNNGTC	1 cut(s)	1160
EaeI	YGGCCR	2 cut(s)	544, 768
Eam1104I	CTCTTC	4 cut(s)	301, 402, 536, 1244
EarI	CTCTTC	4 cut(s)	301, 402, 536, 1244
EciI	GGCGGA	1 cut(s)	1029
Ecl136II	GAGCTC	2 cut(s)	805, 839
Eco130I	CCWWGG	3 cut(s)	765, 810, 948
Eco24I	GRGCYC	3 cut(s)	807, 841, 1224
Eco31I	GGTCTC	2 cut(s)	577, 642
Eco47I	GGWCC	3 cut(s)	372, 1123, 1181
Eco47III	AGCGCT	1 cut(s)	416
Eco53kI	GAGCTC	2 cut(s)	805, 839
Eco57I	CTGAAG	2 cut(s)	1587, 1593
Eco72I	CACGTG	1 cut(s)	491
Eco88I	CYCGRG	2 cut(s)	140, 800
Eco91I	GGTNACC	2 cut(s)	713, 1497
EcoICRI	GAGCTC	2 cut(s)	805, 839
EcoO65I	GGTNACC	2 cut(s)	713, 1497
EcoRI	GAATTC	1 cut(s)	500
EcoRII	CCWGG	2 cut(s)	368, 1290
EcoT14I	CCWWGG	3 cut(s)	765, 810, 948
EcoT38I	GRGCYC	3 cut(s)	807, 841, 1224
ErhI	CCWWGG	3 cut(s)	765, 810, 948
Esp3I	CGTCTC	1 cut(s)	517
FaeI	CATG	3 cut(s)	112, 769, 1153
FaiI	YATR	8 cut(s)	91, 110, 767, 848, 878, 887, 1091, 1151
FalI	AAGNNNNNCTT	8 cut(s)	387, 419, 404, 436, 737, 769, 1199, 1231
FaqI	GGGAC	6 cut(s)	82, 122, 563, 708, 1127, 1167
FatI	CATG	3 cut(s)	108, 765, 1149
FauI	CCCGC	5 cut(s)	278, 293, 702, 1265, 1521
FbaI	TGATCA	1 cut(s)	1435
FblI	GTMKAC	2 cut(s)	384, 792
FokI	GGATG	4 cut(s)	985, 1462, 1546, 1609
FriOI	GRGCYC	3 cut(s)	807, 841, 1224
FspBI	CTAG	2 cut(s)	954, 1409
GlaI	GCGC	6 cut(s)	416, 443, 509, 700, 1131, 1139
GsaI	CCCAGC	3 cut(s)	29, 67, 571
GsuI	CTGGAG	2 cut(s)	29, 446
HaeII	RGCGCY	3 cut(s)	418, 445, 1133
HaeIII	GGCC	7 cut(s)	357, 546, 729, 770, 1059, 1289, 1367
HgaI	GACGC	5 cut(s)	151, 499, 705, 1036, 1155
HhaI	GCGC	6 cut(s)	417, 444, 510, 701, 1132, 1140
Hin1I	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
Hin1II	CATG	3 cut(s)	112, 769, 1153
Hin6I	GCGC	6 cut(s)	415, 442, 508, 699, 1130, 1138
HinP1I	GCGC	6 cut(s)	415, 442, 508, 699, 1130, 1138
HincII	GTYRAC	3 cut(s)	193, 217, 385
HindII	GTYRAC	3 cut(s)	193, 217, 385
HinfI	GANTC	8 cut(s)	95, 344, 398, 560, 1303, 1371, 1643, 1659
Hpy166II	GTNNAC	9 cut(s)	166, 193, 217, 340, 385, 793, 1098, 1123, 1159
Hpy188I	TCNGA	7 cut(s)	820, 829, 906, 1440, 1584, 1642, 1664
Hpy188III	TCNNGA	5 cut(s)	46, 518, 617, 631, 1647
Hpy8I	GTNNAC	9 cut(s)	166, 193, 217, 340, 385, 793, 1098, 1123, 1159
Hpy99I	CGWCG	6 cut(s)	137, 323, 386, 791, 1004, 1522
HpyCH4III	ACNGT	4 cut(s)	40, 319, 391, 1444
HpyCH4IV	ACGT	9 cut(s)	135, 189, 381, 490, 789, 1155, 1161, 1332, 1517
HpyCH4V	TGCA	2 cut(s)	976, 1363
HpyF3I	CTNAG	3 cut(s)	959, 981, 1622
HpySE526I	ACGT	9 cut(s)	135, 189, 381, 490, 789, 1155, 1161, 1332, 1517
Hsp92I	GRCGYC	5 cut(s)	135, 162, 789, 1155, 1517
Hsp92II	CATG	3 cut(s)	112, 769, 1153
HspAI	GCGC	6 cut(s)	415, 442, 508, 699, 1130, 1138
Kpn2I	TCCGGA	1 cut(s)	1646
KroI	GCCGGC	1 cut(s)	1285
KroNI	GCCGGC	1 cut(s)	1287
Ksp22I	TGATCA	1 cut(s)	1435
KspI	CCGCGG	2 cut(s)	355, 1529
Kzo9I	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
LguI	GCTCTTC	1 cut(s)	536
LmnI	GCTCC	7 cut(s)	293, 422, 438, 829, 869, 899, 1414
LweI	GCATC	5 cut(s)	315, 963, 1095, 1216, 1350
MaeI	CTAG	2 cut(s)	954, 1409
MaeII	ACGT	9 cut(s)	135, 189, 381, 490, 789, 1155, 1161, 1332, 1517
MaeIII	GTNAC	8 cut(s)	6, 587, 713, 1024, 1461, 1497, 1513, 1521
MalI	GATC	7 cut(s)	758, 903, 932, 964, 1404, 1437, 1581
MbiI	CCGCTC	2 cut(s)	288, 864
MboI	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
MboII	GAAGA	8 cut(s)	288, 419, 523, 893, 1231, 1555, 1558, 1612
MhlI	GDGCHC	3 cut(s)	807, 841, 1224
MlsI	TGGCCA	1 cut(s)	770
MluCI	AATT	5 cut(s)	265, 500, 937, 1507, 1607
MluNI	TGGCCA	1 cut(s)	770
MlyI	GAGTC	5 cut(s)	104, 338, 392, 554, 1637
MmeI	TCCRAC	3 cut(s)	315, 324, 1665
Mox20I	TGGCCA	1 cut(s)	770
MroI	TCCGGA	1 cut(s)	1646
MroNI	GCCGGC	1 cut(s)	1285
MscI	TGGCCA	1 cut(s)	770
MseI	TTAA	1 cut(s)	1310
MslI	CAYNNNNRTG	2 cut(s)	13, 1091
Msp20I	TGGCCA	1 cut(s)	770
MspA1I	CMGCKG	6 cut(s)	13, 67, 354, 1201, 1250, 1528
MspR9I	CCNGG	5 cut(s)	370, 735, 1127, 1292, 1561
Mva1269I	GAATGC	1 cut(s)	1330
MvaI	CCWGG	2 cut(s)	370, 1292
MvnI	CGCG	5 cut(s)	354, 510, 699, 701, 1528
NaeI	GCCGGC	1 cut(s)	1287
NciI	CCSGG	3 cut(s)	735, 1127, 1561
NcoI	CCATGG	1 cut(s)	765
NdeII	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
NgoMIV	GCCGGC	1 cut(s)	1285
NlaIII	CATG	3 cut(s)	112, 769, 1153
NlaIV	GGNNCC	4 cut(s)	374, 1125, 1174, 1183
NmeAIII	GCCGAG	4 cut(s)	520, 691, 1149, 1424
NmuCI	GTSAC	7 cut(s)	6, 587, 1024, 1461, 1497, 1513, 1521
NspI	RCATGY	1 cut(s)	112
NspV	TTCGAA	2 cut(s)	603, 1242
OliI	CACNNNNGTG	1 cut(s)	13
PaeR7I	CTCGAG	1 cut(s)	800
PaqCI	CACCTGC	1 cut(s)	1184
PciSI	GCTCTTC	1 cut(s)	536
PcsI	WCGNNNNNNNCGW	3 cut(s)	138, 555, 792
PctI	GAATGC	1 cut(s)	1330
PdiI	GCCGGC	1 cut(s)	1287
PfeI	GAWTC	2 cut(s)	1303, 1371
PflFI	GACNNNGTC	4 cut(s)	389, 1030, 1519, 1636
PflMI	CCANNNNNTGG	1 cut(s)	691
PfoI	TCCNGGA	1 cut(s)	368
PleI	GAGTC	5 cut(s)	103, 338, 392, 554, 1637
PmaCI	CACGTG	1 cut(s)	491
PmlI	CACGTG	1 cut(s)	491
PpsI	GAGTC	5 cut(s)	103, 338, 392, 554, 1637
Ppu21I	YACGTR	1 cut(s)	491
Psp124BI	GAGCTC	2 cut(s)	807, 841
Psp6I	CCWGG	2 cut(s)	368, 1290
PspCI	CACGTG	1 cut(s)	491
PspEI	GGTNACC	2 cut(s)	713, 1497
PspFI	CCCAGC	3 cut(s)	25, 63, 567
PspGI	CCWGG	2 cut(s)	368, 1290
PspN4I	GGNNCC	4 cut(s)	374, 1125, 1174, 1183
PspPI	GGNCC	5 cut(s)	372, 728, 1123, 1181, 1288
PspXI	VCTCGAGB	1 cut(s)	800
PsyI	GACNNNGTC	4 cut(s)	389, 1030, 1519, 1636
PvuII	CAGCTG	3 cut(s)	13, 67, 1201
RsaI	GTAC	2 cut(s)	725, 1278
RsaNI	GTAC	2 cut(s)	724, 1277
RseI	CAYNNNNRTG	2 cut(s)	13, 1091
SacI	GAGCTC	2 cut(s)	807, 841
SacII	CCGCGG	2 cut(s)	355, 1529
SalI	GTCGAC	1 cut(s)	383
SapI	GCTCTTC	1 cut(s)	536
SaqAI	TTAA	1 cut(s)	1310
Sau3AI	GATC	7 cut(s)	756, 901, 930, 962, 1402, 1435, 1579
Sau96I	GGNCC	5 cut(s)	372, 728, 1123, 1181, 1288
SchI	GAGTC	5 cut(s)	104, 338, 392, 554, 1637
ScrFI	CCNGG	5 cut(s)	370, 735, 1127, 1292, 1561
SduI	GDGCHC	3 cut(s)	807, 841, 1224
SfaNI	GCATC	5 cut(s)	315, 963, 1095, 1216, 1350
Sfr274I	CTCGAG	1 cut(s)	800
Sfr303I	CCGCGG	2 cut(s)	355, 1529
SfuI	TTCGAA	2 cut(s)	603, 1242
SgrBI	CCGCGG	2 cut(s)	355, 1529
SinI	GGWCC	3 cut(s)	372, 1123, 1181
SlaI	CTCGAG	1 cut(s)	800
SmiMI	CAYNNNNRTG	2 cut(s)	13, 1091
SmlI	CTYRAG	3 cut(s)	428, 629, 800
SmoI	CTYRAG	3 cut(s)	428, 629, 800
Sse9I	AATT	5 cut(s)	265, 500, 937, 1507, 1607
SspMI	CTAG	2 cut(s)	954, 1409
SstI	GAGCTC	2 cut(s)	807, 841
StyD4I	CCNGG	5 cut(s)	368, 733, 1125, 1290, 1559
StyI	CCWWGG	3 cut(s)	765, 810, 948
TaaI	ACNGT	4 cut(s)	40, 319, 391, 1444
TaiI	ACGT	9 cut(s)	138, 192, 384, 493, 792, 1158, 1164, 1335, 1520
TasI	AATT	5 cut(s)	265, 500, 937, 1507, 1607
TauI	GCSGC	4 cut(s)	162, 357, 529, 1556
TfiI	GAWTC	2 cut(s)	1303, 1371
Tru1I	TTAA	1 cut(s)	1310
Tru9I	TTAA	1 cut(s)	1310
TseFI	GTSAC	7 cut(s)	6, 587, 1024, 1461, 1497, 1513, 1521
Tsp45I	GTSAC	7 cut(s)	6, 587, 1024, 1461, 1497, 1513, 1521
TspDTI	ATGAA	4 cut(s)	1095, 1355, 1613, 1619
TspGWI	ACGGA	4 cut(s)	325, 565, 575, 1067
Tth111I	GACNNNGTC	4 cut(s)	389, 1030, 1519, 1636
Van91I	CCANNNNNTGG	1 cut(s)	691
VpaK11BI	GGWCC	3 cut(s)	372, 1123, 1181
XapI	RAATTY	1 cut(s)	500
XceI	RCATGY	1 cut(s)	112
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1375
XhoI	CTCGAG	1 cut(s)	800
XmiI	GTMKAC	2 cut(s)	384, 792
XspI	CTAG	2 cut(s)	954, 1409
ZraI	GACGTC	4 cut(s)	136, 790, 1156, 1518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.