MD03G1001700.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014945
Physical Location & Seq
Reverse (-)
129419 .. 132029
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1001700.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGATCAGAGGCCCAAGACCAAGATCGTCTGCACCCTGGGACCCGCGTCGCGGTCCGTTCCCATGGTGGAGAAGCTGCTGAGCGCCGGCATGAACGTCGCCCGCTTCAACTTCTCCCATGGCTCCCACGAGTACCATCAGGAGACGCTCGACAATCTCAGGGCAGCCATGGAATCCACCGGCATCCTCTGCGCCGTCATGCTCGACACCAAGGGTCCAGAGATTCGAACTGGATTTCTCAAGGATGCGAAACCCGTCCAGCTCAAACTGGGCCAAGAGATCACCATTTCCACTGATTATGGCCTCAAGGGTGATGCAAATATGATTTGTATGAGTTACAAAAAGCTGGCTGAGGATGTCAAGCCGGGGAGCATGATTCTATGTGCTGATGGCACCATCTCGTTTATGGTTCTGTCTTGCGATAAGCTAAAGGGTTTGGTTCAATGCCGCTGTGAGAACTCTGCAGTTCTTGGTGAGAGGAAGAACGTTAATCTTCCCGGGGTCATTGTGGATCTCCCAACCTTAACAGAGAAAGACAAAGAAGATATTCTCAAGTGGGGAGTTCCAAATAAGATTGACATGATTGCCCTGTCTTTTGTTCGGAAAGGCTCTGATCTTGTGGAGGTCAGGAAGCTCCTTGGAAAGCATTCTAAGAATATCCTTCTCATGTCAAAGGTTGAGAATCAGGAAGGGGTGGCAAACTTTGATGACATCCTTGCACAATCAGATGCATTCATGGTGGCACGAGGTGATCTTGGTATGGAAATTCCAATTGAAAAGATCTTCCTTGCACAGAAAGTGATGATCTACAAGTGCAACATCCAAGGAAAACCCGTGGTCACGGCAACACAGATGTTGGAGTCAATGATCAAATCTCCCCGTCCAACTAGGGCTGAAGCTACCGATGTTGCCAATGCCGTTCTAGATGGCACAGATTGTGTGATGCTAAGTGGTGAGACTGCTGCGGGAGCATACCCTGAACTTGCAGTGCGGACCATGGCAAAAATATGCGTAGAAGCAGAGAGCACCCTTCACTATGGAGATGTGTTCAAAAGGATTATGGAACACTCCCCCGTGCCCATGAGCCCACTAGAGAGTCTGGCTTCTTCTGCTGTTAAAACAGCCAACTCGTCCAAAGCAGCTCTTATATTGGTACTGACCAGAGGAGGAAGTACTGCAAAGCTGGTGGCCAAGTACAGACCTGGCATGCCTATACTGTCTGTTGTTGTCCCTGAGATTACGACCGATTCATTTGACTGGTCATGCAGTGATGAAGCTCCAGCAAGGCATAGTCTGATTTTCCGTGGGTTGGTTCCAGTTCTAAGTGCGGGATCTTCTAGGGCCTCTAATGCAGAGACAACTGAAGAAGCGCTGGACTTTGCCCTCCAACATGCCAAGACAAAGGGACTCTGCAAGAACGGAGATGCTGTTGTGGCTCTGCACCGTGATGGAACTGCATCTGTGATCAAGATCTTGACCGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

496

Amino Acids

53.46

Weight (kDa)

7.9

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 6 - 330 7.9e-149 Pyruvate kinase, barrel domain
PK_C PF02887 365 - 488 1.2e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26390 AT5G56350
fragaria_vesca FvH4_3g45920 FvH4_3g45920 FvH4_3g45920
malus_domestica MD03G1001700.v1.1
prunus_persica Prupe.6G001600_v2.0.a1
pyrus_communis pycom11g00160
rosa_chinensis RchiOBHm_Chr5g0084031
rosa_laevigata RLG00000037074
rosa_multiflora Rmu_ssc0000372.1_g000083
rosa_roxburghii Rroxscaffold_1G00000630
rosa_rugosa Rorug05G0488500
rosa_samantha Rh5BG566400 Rh5DG574700
rosa_wichuraiana Rw5G050320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 2 cut(s) 47, 52
AciI CCGC 7 cut(s) 45, 52, 103, 448, 965, 991, 1328
AclI AACGTT 1 cut(s) 486
AclWI GGATC 3 cut(s) 12, 519, 1339
AcoI YGGCCR 1 cut(s) 1188
AcsI RAATTY 1 cut(s) 765
AcuI CTGAAG 2 cut(s) 915, 1383
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 4 cut(s) 134, 1155, 1174, 1196
AfeI AGCGCT 1 cut(s) 1371
AfiI CCNNNNNNNGG 2 cut(s) 51, 1309
AgsI TTSAA 4 cut(s) 109, 443, 776, 1051
AjnI CCWGG 2 cut(s) 36, 1201
AluBI AGCT 9 cut(s) 76, 262, 346, 427, 634, 899, 1142, 1183, 1277
AluI AGCT 9 cut(s) 76, 262, 346, 427, 634, 899, 1142, 1183, 1277
Alw21I GWGCWC 1 cut(s) 1028
Alw26I GTCTC 3 cut(s) 137, 950, 1349
AlwI GGATC 3 cut(s) 12, 519, 1339
Ama87I CYCGRG 1 cut(s) 497
Aor51HI AGCGCT 1 cut(s) 1371
AoxI GGCC 5 cut(s) 11, 271, 301, 1188, 1341
ApeKI GCWGC 4 cut(s) 76, 164, 962, 1139
ApoI RAATTY 1 cut(s) 765
Asp700I GAANNNNTTC 2 cut(s) 546, 646
AspLEI GCGC 3 cut(s) 86, 194, 1372
AspS9I GGNCC 7 cut(s) 12, 41, 54, 215, 271, 993, 1341
AsuC2I CCSGG 3 cut(s) 366, 498, 499
AsuHPI GGTGA 5 cut(s) 274, 323, 485, 761, 965
AsuII TTCGAA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 4 cut(s) 41, 54, 215, 993
BaeGI GKGCMC 1 cut(s) 1080
BalI TGGCCA 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 1088
BauI CACGAG 2 cut(s) 128, 744
Bbv12I GWGCWC 1 cut(s) 1028
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 63, 176, 949, 1151
BccI CCATC 5 cut(s) 144, 383, 404, 920, 1442
BceAI ACGGC 3 cut(s) 179, 858, 902
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 2 cut(s) 38, 1203
BclI TGATCA 2 cut(s) 867, 1464
BcnI CCSGG 3 cut(s) 366, 498, 499
BcoDI GTCTC 3 cut(s) 137, 950, 1349
BfaI CTAG 4 cut(s) 888, 923, 1091, 1338
BfmI CTRYAG 1 cut(s) 462
BfoI RGCGCY 2 cut(s) 87, 1373
BglII AGATCT 2 cut(s) 780, 1470
BisI GCNGC 5 cut(s) 77, 165, 448, 963, 1140
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 5 cut(s) 78, 166, 449, 964, 1141
BmcAI AGTACT 1 cut(s) 1174
Bme1390I CCNGG 5 cut(s) 38, 366, 498, 499, 1203
Bme18I GGWCC 4 cut(s) 41, 54, 215, 993
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 7 cut(s) 12, 41, 54, 215, 271, 993, 1341
BmiI GGNNCC 6 cut(s) 42, 43, 124, 216, 394, 1314
BmrFI CCNGG 5 cut(s) 38, 366, 498, 499, 1203
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 7 cut(s) 192, 235, 304, 718, 933, 1414, 1466
BmuI ACTGGG 1 cut(s) 278
BoxI GACNNNNGTC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 1263
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 3 cut(s) 224, 290, 536
BpuMI CCSGG 3 cut(s) 366, 498, 499
BsaBI GATNNNNATC 1 cut(s) 1469
BsaXI ACNNNNNCTCC 2 cut(s) 1413, 1443
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 1309
Bse118I RCCGGY 2 cut(s) 86, 179
Bse1I ACTGG 4 cut(s) 235, 273, 1262, 1316
Bse8I GATNNNNATC 1 cut(s) 1469
BseBI CCWGG 2 cut(s) 38, 1203
BseGI GGATG 5 cut(s) 183, 250, 361, 711, 819
BseJI GATNNNNATC 1 cut(s) 1469
BseLI CCNNNNNNNGG 2 cut(s) 51, 1309
BseMII CTCAG 4 cut(s) 71, 172, 342, 1224
BseNI ACTGG 4 cut(s) 235, 273, 1262, 1316
BseRI GAGGAG 1 cut(s) 1179
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 4 cut(s) 63, 176, 949, 1151
BsgI GTGCAG 2 cut(s) 16, 1424
Bsh1236I CGCG 2 cut(s) 47, 52
Bsh1285I CGRYCG 1 cut(s) 1245
BshFI GGCC 5 cut(s) 13, 273, 303, 1190, 1343
BshNI GGYRCC 1 cut(s) 392
BsiEI CGRYCG 1 cut(s) 1245
BsiHKAI GWGCWC 1 cut(s) 1028
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 4 cut(s) 87, 180, 365, 498
BslFI GGGAC 3 cut(s) 54, 1214, 1419
BslI CCNNNNNNNGG 2 cut(s) 51, 1309
BsmAI GTCTC 3 cut(s) 137, 950, 1349
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 3 cut(s) 54, 1214, 1419
BsmI GAATGC 2 cut(s) 646, 731
BsnI GGCC 5 cut(s) 13, 273, 303, 1190, 1343
BsoBI CYCGRG 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 226
Bsp1286I GDGCHC 3 cut(s) 1028, 1080, 1088
Bsp1720I GCTNAGC 1 cut(s) 80
Bsp19I CCATGG 4 cut(s) 63, 118, 168, 996
BspACI CCGC 7 cut(s) 45, 52, 103, 448, 965, 991, 1328
BspANI GGCC 5 cut(s) 13, 273, 303, 1190, 1343
BspCNI CTCAG 4 cut(s) 72, 171, 343, 1225
BspFNI CGCG 2 cut(s) 47, 52
BspLI GGNNCC 6 cut(s) 42, 43, 124, 216, 394, 1314
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 3 cut(s) 12, 519, 1339
BspT104I TTCGAA 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 392
BsrFI RCCGGY 2 cut(s) 86, 179
BsrI ACTGG 4 cut(s) 235, 273, 1262, 1316
BssAI RCCGGY 2 cut(s) 86, 179
BssSI CACGAG 2 cut(s) 128, 744
BssT1I CCWWGG 7 cut(s) 63, 118, 168, 210, 637, 823, 996
Bst2BI CACGAG 2 cut(s) 128, 744
Bst2UI CCWGG 2 cut(s) 38, 1203
Bst4CI ACNGT 3 cut(s) 1218, 1445, 1480
BstAPI GCANNNNNTGC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 88, 103, 348, 1208
BstDEI CTNAG 7 cut(s) 80, 158, 351, 651, 947, 1233, 1322
BstDSI CCRYGG 6 cut(s) 63, 118, 168, 834, 996, 1303
BstF5I GGATG 5 cut(s) 183, 250, 361, 711, 819
BstFNI CGCG 2 cut(s) 47, 52
BstH2I RGCGCY 2 cut(s) 87, 1373
BstHHI GCGC 3 cut(s) 86, 194, 1372
BstMAI GTCTC 3 cut(s) 137, 950, 1349
BstMCI CGRYCG 1 cut(s) 1245
BstMWI GCNNNNNNNGC 5 cut(s) 189, 968, 1109, 1349, 1433
BstNI CCWGG 2 cut(s) 38, 1203
BstNSI RCATGY 2 cut(s) 1210, 1394
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 5 cut(s) 36, 364, 496, 497, 1201
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 2 cut(s) 47, 52
BstV1I GCAGC 4 cut(s) 63, 176, 949, 1151
BstX2I RGATCY 4 cut(s) 511, 780, 1331, 1470
BstYI RGATCY 4 cut(s) 511, 780, 1331, 1470
BsuRI GGCC 5 cut(s) 13, 273, 303, 1190, 1343
BtgI CCRYGG 6 cut(s) 63, 118, 168, 834, 996, 1303
BtsCI GGATG 5 cut(s) 183, 250, 361, 711, 819
BtsI GCAGTG 2 cut(s) 993, 1273
BtsIMutI CAGTG 3 cut(s) 291, 993, 1273
Cac8I GCNNGC 4 cut(s) 88, 103, 348, 1208
CfoI GCGC 3 cut(s) 86, 194, 1372
Cfr10I RCCGGY 2 cut(s) 86, 179
Cfr13I GGNCC 7 cut(s) 12, 41, 54, 215, 271, 993, 1341
Cfr9I CCCGGG 1 cut(s) 497
CpoI CGGWCCG 1 cut(s) 54
CseI GACGC 2 cut(s) 36, 154
Csp6I GTAC 4 cut(s) 133, 1154, 1173, 1195
CspCI CAANNNNNGTGG 2 cut(s) 1167, 1202
CspI CGGWCCG 1 cut(s) 54
CviQI GTAC 4 cut(s) 133, 1154, 1173, 1195
DdeI CTNAG 7 cut(s) 80, 158, 351, 651, 947, 1233, 1322
DraIII CACNNNGTG 1 cut(s) 749
EaeI YGGCCR 1 cut(s) 1188
Eco130I CCWWGG 7 cut(s) 63, 118, 168, 210, 637, 823, 996
Eco24I GRGCYC 1 cut(s) 1088
Eco47I GGWCC 4 cut(s) 41, 54, 215, 993
Eco47III AGCGCT 1 cut(s) 1371
Eco57I CTGAAG 2 cut(s) 915, 1383
Eco88I CYCGRG 1 cut(s) 497
EcoO109I RGGNCCY 2 cut(s) 41, 1341
EcoRII CCWGG 2 cut(s) 36, 1201
EcoT14I CCWWGG 7 cut(s) 63, 118, 168, 210, 637, 823, 996
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 1088
ErhI CCWWGG 7 cut(s) 63, 118, 168, 210, 637, 823, 996
Esp3I CGTCTC 1 cut(s) 137
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FaqI GGGAC 3 cut(s) 54, 1214, 1419
FauI CCCGC 4 cut(s) 52, 110, 958, 1321
FbaI TGATCA 2 cut(s) 867, 1464
Fnu4HI GCNGC 5 cut(s) 77, 165, 448, 963, 1140
FokI GGATG 5 cut(s) 170, 257, 368, 698, 806
FriOI GRGCYC 1 cut(s) 1088
Fsp4HI GCNGC 5 cut(s) 77, 165, 448, 963, 1140
FspBI CTAG 4 cut(s) 888, 923, 1091, 1338
GlaI GCGC 3 cut(s) 85, 193, 1371
GluI GCNGC 5 cut(s) 77, 165, 448, 963, 1140
GsuI CTGGAG 1 cut(s) 1263
HaeII RGCGCY 2 cut(s) 87, 1373
HaeIII GGCC 5 cut(s) 13, 273, 303, 1190, 1343
HapII CCGG 4 cut(s) 87, 180, 365, 498
HgaI GACGC 2 cut(s) 36, 154
HhaI GCGC 3 cut(s) 86, 194, 1372
Hin6I GCGC 3 cut(s) 84, 192, 1370
HinP1I GCGC 3 cut(s) 84, 192, 1370
HinfI GANTC 8 cut(s) 173, 223, 376, 682, 860, 1096, 1247, 1407
HpaII CCGG 4 cut(s) 87, 180, 365, 498
HphI GGTGA 5 cut(s) 274, 323, 485, 761, 965
Hpy188I TCNGA 5 cut(s) 9, 603, 613, 727, 1296
Hpy188III TCNNGA 7 cut(s) 140, 218, 628, 686, 923, 1468, 1474
Hpy99I CGWCG 2 cut(s) 52, 101
HpyAV CCTTC 3 cut(s) 671, 683, 1040
HpyCH4III ACNGT 3 cut(s) 1218, 1445, 1480
HpyCH4IV ACGT 2 cut(s) 96, 486
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 968, 1109, 1349, 1433
HpyF3I CTNAG 7 cut(s) 80, 158, 351, 651, 947, 1233, 1322
HpySE526I ACGT 2 cut(s) 96, 486
HspAI GCGC 3 cut(s) 84, 192, 1370
KflI GGGWCCC 1 cut(s) 41
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Ksp22I TGATCA 2 cut(s) 867, 1464
LmnI GCTCC 5 cut(s) 128, 369, 639, 968, 1282
Lsp1109I GCAGC 4 cut(s) 63, 176, 949, 1151
LweI GCATC 7 cut(s) 192, 235, 304, 718, 933, 1414, 1466
MaeI CTAG 4 cut(s) 888, 923, 1091, 1338
MaeII ACGT 2 cut(s) 96, 486
MaeIII GTNAC 2 cut(s) 335, 838
MboII GAAGA 7 cut(s) 485, 493, 554, 775, 1098, 1326, 1376
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 4 cut(s) 511, 780, 1331, 1470
MhlI GDGCHC 3 cut(s) 1028, 1080, 1088
MlsI TGGCCA 1 cut(s) 1190
MluCI AATT 2 cut(s) 765, 771
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 3 cut(s) 869, 1105, 1401
MmeI TCCRAC 3 cut(s) 837, 908, 1411
Mox20I TGGCCA 1 cut(s) 1190
Mph1103I ATGCAT 1 cut(s) 733
MroNI GCCGGC 1 cut(s) 86
MroXI GAANNNNTTC 2 cut(s) 546, 646
MscI TGGCCA 1 cut(s) 1190
MseI TTAA 3 cut(s) 489, 524, 1116
MslI CAYNNNNRTG 1 cut(s) 1446
Msp20I TGGCCA 1 cut(s) 1190
MspA1I CMGCKG 1 cut(s) 450
MspI CCGG 4 cut(s) 87, 180, 365, 498
MspR9I CCNGG 5 cut(s) 38, 366, 498, 499, 1203
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 2 cut(s) 646, 731
MvaI CCWGG 2 cut(s) 38, 1203
MvnI CGCG 2 cut(s) 47, 52
MwoI GCNNNNNNNGC 5 cut(s) 189, 968, 1109, 1349, 1433
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 3 cut(s) 366, 498, 499
NcoI CCATGG 4 cut(s) 63, 118, 168, 996
NgoMIV GCCGGC 1 cut(s) 86
NlaIV GGNNCC 6 cut(s) 42, 43, 124, 216, 394, 1314
NmuCI GTSAC 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 733
NspI RCATGY 2 cut(s) 1210, 1394
NspV TTCGAA 1 cut(s) 226
PaeI GCATGC 1 cut(s) 1210
PasI CCCWGGG 1 cut(s) 37
PctI GAATGC 2 cut(s) 646, 731
PdiI GCCGGC 1 cut(s) 88
PdmI GAANNNNTTC 2 cut(s) 546, 646
PfeI GAWTC 5 cut(s) 173, 223, 376, 682, 1247
PkrI GCNGC 5 cut(s) 78, 166, 449, 964, 1141
PleI GAGTC 3 cut(s) 868, 1104, 1401
PpsI GAGTC 3 cut(s) 868, 1104, 1401
PpuMI RGGWCCY 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 46
Psp1406I AACGTT 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 36, 1201
PspGI CCWGG 2 cut(s) 36, 1201
PspN4I GGNNCC 6 cut(s) 42, 43, 124, 216, 394, 1314
PspPI GGNCC 7 cut(s) 12, 41, 54, 215, 271, 993, 1341
PspPPI RGGWCCY 1 cut(s) 41
PstI CTGCAG 1 cut(s) 466
PsuI RGATCY 4 cut(s) 511, 780, 1331, 1470
RsaI GTAC 4 cut(s) 134, 1155, 1174, 1196
RsaNI GTAC 4 cut(s) 133, 1154, 1173, 1195
RseI CAYNNNNRTG 1 cut(s) 1446
Rsr2I CGGWCCG 1 cut(s) 54
RsrII CGGWCCG 1 cut(s) 54
SaqAI TTAA 3 cut(s) 489, 524, 1116
SatI GCNGC 5 cut(s) 77, 165, 448, 963, 1140
Sau96I GGNCC 7 cut(s) 12, 41, 54, 215, 271, 993, 1341
ScaI AGTACT 1 cut(s) 1174
SchI GAGTC 3 cut(s) 869, 1105, 1401
ScrFI CCNGG 5 cut(s) 38, 366, 498, 499, 1203
SduI GDGCHC 3 cut(s) 1028, 1080, 1088
SfaNI GCATC 7 cut(s) 192, 235, 304, 718, 933, 1414, 1466
SfcI CTRYAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 4 cut(s) 41, 54, 215, 993
SmaI CCCGGG 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 1446
SmlI CTYRAG 3 cut(s) 239, 305, 551
SmoI CTYRAG 3 cut(s) 239, 305, 551
SphI GCATGC 1 cut(s) 1210
Sse9I AATT 2 cut(s) 765, 771
SsiI CCGC 7 cut(s) 45, 52, 103, 448, 965, 991, 1328
SspMI CTAG 4 cut(s) 888, 923, 1091, 1338
StyD4I CCNGG 5 cut(s) 36, 364, 496, 497, 1201
StyI CCWWGG 7 cut(s) 63, 118, 168, 210, 637, 823, 996
TaaI ACNGT 3 cut(s) 1218, 1445, 1480
TaiI ACGT 2 cut(s) 99, 489
TaqI TCGA 3 cut(s) 150, 204, 226
TaqII GACCGA 1 cut(s) 1259
TasI AATT 2 cut(s) 765, 771
TatI WGTACW 2 cut(s) 1172, 1194
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 5 cut(s) 173, 223, 376, 682, 1247
Tru1I TTAA 3 cut(s) 489, 524, 1116
Tru9I TTAA 3 cut(s) 489, 524, 1116
TscAI CASTG 3 cut(s) 298, 993, 1273
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 4 cut(s) 76, 164, 962, 1139
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 4 cut(s) 107, 724, 1239, 1287
TspGWI ACGGA 3 cut(s) 46, 1292, 1434
TspMI CCCGGG 1 cut(s) 497
TspRI CASTG 3 cut(s) 298, 993, 1273
VpaK11BI GGWCC 4 cut(s) 41, 54, 215, 993
XapI RAATTY 1 cut(s) 765
XbaI TCTAGA 1 cut(s) 922
XceI RCATGY 2 cut(s) 1210, 1394
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmaI CCCGGG 1 cut(s) 497
XmnI GAANNNNTTC 2 cut(s) 546, 646
XspI CTAG 4 cut(s) 888, 923, 1091, 1338
ZrmI AGTACT 1 cut(s) 1174
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.