Rw5G050320

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014945
Physical Location & Seq
Forward (+)
86881903 .. 86884754
2852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050320.1

Sequence Viewer

Length: 1488 bp
ATGGATCAGAAGCCGAAGACGAAGATCGTGTGCACCCTGGGACCTGCATCCAGGTCGGTGCCGATGGTCGAGAAGCTTCTCCGTGCCGGAATGAACGTGGCCCGCTTCAACTTCTCCCATGGCTCCCACGAGTACCATCAGGAGACCCTCAACAACCTCAGGGCCGCCATGGATTCCACCGGAATTCTCTGCGCCGTCATGCTCGATACCAAGGGTCCGGAGATTCGAACTGGATTTTTGAAGGATGGCAAACCCATCCAGCTCAAACAGGGTCAAGAAATCACCATATCCACAGACTACAGCCTCAAGGGTGATGAAAATGTGATTTGCATGAGCTACAAGAAGTTGGCTGAGGATGTCAAGCCAGGGAGCATGATACTGTGTGCTGATGGTACCATCTCATTTTTGGCTTTATCTTGTGACAAAGCAAAGGGCCTCGTCAGATGCCGATGTGAGAACTCTGCCATGCTCGGTGAGAGAAAGAATGTTAATCTTCCAGGGGTTGTTGTGGATCTCCCTACCTTAACAGAAAAGGACAAAGAAGATATTCTCAAGTGGGGGATTCCAAACAAGATTGACATGATTGCTCTGTCTTTTGTTCGAAAAGGTTCAGATCTAGTGGAGGTTCGCAAGCTCCTTGGAAAGCATGCTAAGAACATCCTTCTCATGTCAAAGGTGGAGAATCAGGAAGGAGTTGCAAACTTTGATGATATCCTTGCAAATTCAGATGCATTCATGGTGGCACGAGGTGATCTTGGAATGGAAATTCCAATTGAAAAGATCTTTCTGGCTCAGAAAGTGATGATCTATAAGTGCAACATCCAAGGAAAACCTGTTGTCACGGCAACACAGATGTTGGAATCAATGATCAAGTCTCCCCGTCCAACTAGGGCTGAAGCTACTGATGTTGCAAATGCAGTTCTAGATGGCACAGACTGTGTGATGTTAAGTGGTGAGACTGCTGCTGGAGCTTATCCTGAACTTGCAGTGCGCACCATGGCAAAAATATGCCTAGAAGCAGAAAGCACCCTTCACTATGGAGATGTCTTCAAAAGGATTATGGAGCACTCCCCGGTGCCCATGAGCCCATTAGAGAGCCTGGCTTCTTCTGCCGTTAAAACAGCCAACTCATCCAAAGCGGCTCTAATTTTGGTCTTAACCAGAGGAGGAAGTACTGCAAAACTAGTGGCCAAGTACAGGCCAGGCATGCCTATACTGTCTGTTGTTGTCCCTGAGATTAAGACTGACTCTTTTGACTGGTCATGCAGTGATGAAGCTCCAGCAAGGCATAGTCTAATTTTCCGTGGCTTGGTTCCAGTCTTAAGTGCAGGATCATCTAGGGCCTCTCATGCAGAAACAACTGAACAAGCTCTGGAGTTTGCCATTCAACATGCTAAGGCAAAGGGACTGTGCAAGAATGGAGATGCTGTTGTGGCTCTGCATCGTGATGGAAATGCATCTGTGATCAAGATCTTGACTGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

495

Amino Acids

53.56

Weight (kDa)

8.58

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 6 - 330 3.9e-149 Pyruvate kinase, barrel domain
PK_C PF02887 365 - 483 1.8e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26390 AT5G56350
fragaria_vesca FvH4_3g45920 FvH4_3g45920 FvH4_3g45920
malus_domestica MD03G1001700.v1.1
prunus_persica Prupe.6G001600_v2.0.a1
pyrus_communis pycom11g00160
rosa_chinensis RchiOBHm_Chr5g0084031
rosa_laevigata RLG00000037074
rosa_multiflora Rmu_ssc0000372.1_g000083
rosa_roxburghii Rroxscaffold_1G00000630
rosa_rugosa Rorug05G0488500
rosa_samantha Rh5BG566400 Rh5DG574700
rosa_wichuraiana Rw5G050320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 992
Acc36I ACCTGC 1 cut(s) 52
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 3 cut(s) 58, 392, 1075
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 3 cut(s) 103, 165, 1139
AclWI GGATC 3 cut(s) 12, 519, 1339
AcoI YGGCCR 1 cut(s) 1188
AcsI RAATTY 3 cut(s) 183, 721, 765
AcuI CTGAAG 1 cut(s) 915
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 4 cut(s) 134, 394, 1174, 1196
AfiI CCNNNNNNNGG 2 cut(s) 1197, 1309
AflII CTTAAG 1 cut(s) 1321
AgsI TTSAA 5 cut(s) 109, 241, 776, 1051, 1388
AhlI ACTAGT 1 cut(s) 1183
AjnI CCWGG 6 cut(s) 36, 50, 364, 496, 1098, 1201
AluBI AGCT 8 cut(s) 76, 262, 336, 634, 899, 971, 1277, 1370
AluI AGCT 8 cut(s) 76, 262, 336, 634, 899, 971, 1277, 1370
Alw21I GWGCWC 2 cut(s) 35, 1068
Alw26I GTCTC 3 cut(s) 137, 879, 950
Alw44I GTGCAC 1 cut(s) 31
AlwI GGATC 3 cut(s) 12, 519, 1339
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 6 cut(s) 99, 162, 433, 1188, 1199, 1341
ApaLI GTGCAC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 962
ApoI RAATTY 3 cut(s) 183, 721, 765
Asp700I GAANNNNTTC 2 cut(s) 546, 607
Asp718I GGTACC 1 cut(s) 392
AspLEI GCGC 2 cut(s) 194, 993
AspS9I GGNCC 6 cut(s) 41, 100, 162, 215, 433, 1341
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 5 cut(s) 274, 323, 485, 761, 965
AsuII TTCGAA 2 cut(s) 226, 601
AvaII GGWCC 2 cut(s) 41, 215
AxyI CCTNAGG 1 cut(s) 158
BaeGI GKGCMC 2 cut(s) 35, 1080
BalI TGGCCA 1 cut(s) 1190
BanI GGYRCC 3 cut(s) 58, 392, 1075
BanII GRGCYC 1 cut(s) 1088
BauI CACGAG 2 cut(s) 128, 744
BbsI GAAGAC 2 cut(s) 23, 1039
Bbv12I GWGCWC 2 cut(s) 35, 1068
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 949
BccI CCATC 8 cut(s) 58, 144, 239, 263, 383, 404, 920, 1442
BceAI ACGGC 3 cut(s) 179, 858, 1097
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BciT130I CCWGG 6 cut(s) 38, 52, 366, 498, 1100, 1203
BclI TGATCA 2 cut(s) 867, 1464
BcnI CCSGG 1 cut(s) 1073
BcoDI GTCTC 3 cut(s) 137, 879, 950
BcuI ACTAGT 1 cut(s) 1183
BfaI CTAG 6 cut(s) 617, 888, 923, 1013, 1184, 1338
BfmI CTRYAG 1 cut(s) 298
BfrI CTTAAG 1 cut(s) 1321
BfuAI ACCTGC 1 cut(s) 52
BglII AGATCT 3 cut(s) 613, 780, 1470
BisI GCNGC 3 cut(s) 165, 963, 1140
BlsI GCNGC 3 cut(s) 166, 964, 1141
BmcAI AGTACT 1 cut(s) 1174
Bme1390I CCNGG 7 cut(s) 38, 52, 366, 498, 1073, 1100, 1203
Bme18I GGWCC 2 cut(s) 41, 215
BmgT120I GGNCC 6 cut(s) 41, 100, 162, 215, 433, 1341
BmiI GGNNCC 7 cut(s) 42, 60, 124, 216, 394, 1077, 1314
BmrFI CCNGG 7 cut(s) 38, 52, 366, 498, 1073, 1100, 1203
BmsI GCATC 6 cut(s) 56, 434, 718, 1414, 1450, 1466
BpiI GAAGAC 2 cut(s) 23, 1039
BpmI CTGGAG 3 cut(s) 987, 1263, 1394
Bpu10I CCTNAGC 2 cut(s) 351, 1395
Bpu14I TTCGAA 2 cut(s) 226, 601
BpuEI CTTGAG 2 cut(s) 290, 536
BpuMI CCSGG 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 1469
BsaI GGTCTC 1 cut(s) 137
BsaWI WCCGGW 2 cut(s) 179, 217
BsaXI ACNNNNNCTCC 2 cut(s) 1413, 1443
Bsc4I CCNNNNNNNGG 2 cut(s) 1197, 1309
Bse1I ACTGG 3 cut(s) 235, 1262, 1316
Bse21I CCTNAGG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 1469
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 6 cut(s) 38, 52, 366, 498, 1100, 1203
BseGI GGATG 7 cut(s) 47, 250, 255, 361, 657, 819, 1130
BseJI GATNNNNATC 1 cut(s) 1469
BseLI CCNNNNNNNGG 2 cut(s) 1197, 1309
BseMII CTCAG 4 cut(s) 172, 342, 806, 1224
BseNI ACTGG 3 cut(s) 235, 1262, 1316
BseRI GAGGAG 1 cut(s) 1179
BseSI GKGCMC 2 cut(s) 35, 1080
BseXI GCAGC 1 cut(s) 949
BsgI GTGCAG 1 cut(s) 1347
BshFI GGCC 6 cut(s) 101, 164, 435, 1190, 1201, 1343
BshNI GGYRCC 3 cut(s) 58, 392, 1075
BsiHKAI GWGCWC 2 cut(s) 35, 1068
BsiSI CCGG 4 cut(s) 87, 180, 218, 1073
BslFI GGGAC 3 cut(s) 54, 1214, 1419
BslI CCNNNNNNNGG 2 cut(s) 1197, 1309
BsmAI GTCTC 3 cut(s) 137, 879, 950
BsmFI GGGAC 3 cut(s) 54, 1214, 1419
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 6 cut(s) 101, 164, 435, 1190, 1201, 1343
Bso31I GGTCTC 1 cut(s) 137
Bsp119I TTCGAA 2 cut(s) 226, 601
Bsp1286I GDGCHC 4 cut(s) 35, 1068, 1080, 1088
Bsp13I TCCGGA 1 cut(s) 217
Bsp19I CCATGG 3 cut(s) 118, 168, 996
BspACI CCGC 3 cut(s) 103, 165, 1139
BspANI GGCC 6 cut(s) 101, 164, 435, 1190, 1201, 1343
BspCNI CTCAG 4 cut(s) 171, 343, 805, 1225
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 7 cut(s) 42, 60, 124, 216, 394, 1077, 1314
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 3 cut(s) 12, 519, 1339
BspT104I TTCGAA 2 cut(s) 226, 601
BspT107I GGYRCC 3 cut(s) 58, 392, 1075
BspTI CTTAAG 1 cut(s) 1321
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 3 cut(s) 235, 1262, 1316
BssSI CACGAG 2 cut(s) 128, 744
BssT1I CCWWGG 6 cut(s) 118, 168, 210, 637, 823, 996
Bst2BI CACGAG 2 cut(s) 128, 744
Bst2UI CCWGG 6 cut(s) 38, 52, 366, 498, 1100, 1203
Bst4CI ACNGT 5 cut(s) 381, 938, 1218, 1410, 1480
BstAFI CTTAAG 1 cut(s) 1321
BstBI TTCGAA 2 cut(s) 226, 601
BstC8I GCNNGC 4 cut(s) 103, 632, 648, 1208
BstDEI CTNAG 6 cut(s) 158, 351, 651, 792, 1233, 1395
BstDSI CCRYGG 4 cut(s) 118, 168, 996, 1303
BstF5I GGATG 7 cut(s) 47, 250, 255, 361, 657, 819, 1130
BstHHI GCGC 2 cut(s) 194, 993
BstMAI GTCTC 3 cut(s) 137, 879, 950
BstMWI GCNNNNNNNGC 5 cut(s) 968, 1109, 1207, 1349, 1433
BstNI CCWGG 6 cut(s) 38, 52, 366, 498, 1100, 1203
BstNSI RCATGY 3 cut(s) 650, 1210, 1394
BstSCI CCNGG 7 cut(s) 36, 50, 364, 496, 1071, 1098, 1201
BstSFI CTRYAG 1 cut(s) 298
BstSLI GKGCMC 2 cut(s) 35, 1080
BstV1I GCAGC 1 cut(s) 949
BstV2I GAAGAC 2 cut(s) 23, 1039
BstX2I RGATCY 4 cut(s) 511, 613, 780, 1470
BstYI RGATCY 4 cut(s) 511, 613, 780, 1470
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 6 cut(s) 101, 164, 435, 1190, 1201, 1343
BtgI CCRYGG 4 cut(s) 118, 168, 996, 1303
BtsCI GGATG 7 cut(s) 47, 250, 255, 361, 657, 819, 1130
BtsI GCAGTG 2 cut(s) 993, 1273
BtsIMutI CAGTG 2 cut(s) 993, 1273
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 4 cut(s) 103, 632, 648, 1208
CfoI GCGC 2 cut(s) 194, 993
Cfr13I GGNCC 6 cut(s) 41, 100, 162, 215, 433, 1341
Csp6I GTAC 4 cut(s) 133, 393, 1173, 1195
CspCI CAANNNNNGTGG 2 cut(s) 1167, 1202
CviQI GTAC 4 cut(s) 133, 393, 1173, 1195
DdeI CTNAG 6 cut(s) 158, 351, 651, 792, 1233, 1395
DraIII CACNNNGTG 1 cut(s) 749
EaeI YGGCCR 1 cut(s) 1188
Eco130I CCWWGG 6 cut(s) 118, 168, 210, 637, 823, 996
Eco24I GRGCYC 1 cut(s) 1088
Eco31I GGTCTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 2 cut(s) 41, 215
Eco57I CTGAAG 1 cut(s) 915
Eco81I CCTNAGG 1 cut(s) 158
EcoO109I RGGNCCY 3 cut(s) 41, 433, 1341
EcoRI GAATTC 1 cut(s) 183
EcoRII CCWGG 6 cut(s) 36, 50, 364, 496, 1098, 1201
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 6 cut(s) 118, 168, 210, 637, 823, 996
EcoT22I ATGCAT 2 cut(s) 733, 1459
EcoT38I GRGCYC 1 cut(s) 1088
ErhI CCWWGG 6 cut(s) 118, 168, 210, 637, 823, 996
FaqI GGGAC 3 cut(s) 54, 1214, 1419
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 2 cut(s) 867, 1464
Fnu4HI GCNGC 3 cut(s) 165, 963, 1140
FokI GGATG 7 cut(s) 34, 242, 257, 368, 644, 806, 1117
FriOI GRGCYC 1 cut(s) 1088
Fsp4HI GCNGC 3 cut(s) 165, 963, 1140
FspAI RTGCGCAY 1 cut(s) 992
FspBI CTAG 6 cut(s) 617, 888, 923, 1013, 1184, 1338
FspI TGCGCA 1 cut(s) 992
GlaI GCGC 2 cut(s) 193, 992
GluI GCNGC 3 cut(s) 165, 963, 1140
GsuI CTGGAG 3 cut(s) 987, 1263, 1394
HaeIII GGCC 6 cut(s) 101, 164, 435, 1190, 1201, 1343
HapII CCGG 4 cut(s) 87, 180, 218, 1073
HhaI GCGC 2 cut(s) 194, 993
Hin6I GCGC 2 cut(s) 192, 991
HinP1I GCGC 2 cut(s) 192, 991
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 6 cut(s) 173, 223, 562, 682, 860, 1247
HpaII CCGG 4 cut(s) 87, 180, 218, 1073
HphI GGTGA 5 cut(s) 274, 323, 485, 761, 965
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 5 cut(s) 9, 443, 613, 727, 795
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 4 cut(s) 235, 671, 683, 1040
HpyCH4III ACNGT 5 cut(s) 381, 938, 1218, 1410, 1480
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 5 cut(s) 968, 1109, 1207, 1349, 1433
HpyF3I CTNAG 6 cut(s) 158, 351, 651, 792, 1233, 1395
HpySE526I ACGT 1 cut(s) 96
HspAI GCGC 2 cut(s) 192, 991
Kpn2I TCCGGA 1 cut(s) 217
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 2 cut(s) 867, 1464
LmnI GCTCC 6 cut(s) 128, 369, 639, 968, 1063, 1282
Lsp1109I GCAGC 1 cut(s) 949
LweI GCATC 6 cut(s) 56, 434, 718, 1414, 1450, 1466
MaeI CTAG 6 cut(s) 617, 888, 923, 1013, 1184, 1338
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 419, 838
MboII GAAGA 6 cut(s) 28, 34, 485, 554, 1039, 1098
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 4 cut(s) 511, 613, 780, 1470
MhlI GDGCHC 4 cut(s) 35, 1068, 1080, 1088
MlsI TGGCCA 1 cut(s) 1190
MluCI AATT 6 cut(s) 183, 721, 765, 771, 1146, 1296
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 1 cut(s) 1241
MmeI TCCRAC 2 cut(s) 837, 908
Mox20I TGGCCA 1 cut(s) 1190
Mph1103I ATGCAT 2 cut(s) 733, 1459
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 2 cut(s) 546, 607
MscI TGGCCA 1 cut(s) 1190
MseI TTAA 7 cut(s) 489, 524, 947, 1116, 1157, 1239, 1322
MslI CAYNNNNRTG 1 cut(s) 1446
Msp20I TGGCCA 1 cut(s) 1190
MspCI CTTAAG 1 cut(s) 1321
MspI CCGG 4 cut(s) 87, 180, 218, 1073
MspR9I CCNGG 7 cut(s) 38, 52, 366, 498, 1073, 1100, 1203
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 6 cut(s) 38, 52, 366, 498, 1100, 1203
MwoI GCNNNNNNNGC 5 cut(s) 968, 1109, 1207, 1349, 1433
NciI CCSGG 1 cut(s) 1073
NcoI CCATGG 3 cut(s) 118, 168, 996
NlaIV GGNNCC 7 cut(s) 42, 60, 124, 216, 394, 1077, 1314
NmuCI GTSAC 2 cut(s) 419, 838
NsbI TGCGCA 1 cut(s) 992
NsiI ATGCAT 2 cut(s) 733, 1459
NspI RCATGY 3 cut(s) 650, 1210, 1394
NspV TTCGAA 2 cut(s) 226, 601
PaeI GCATGC 2 cut(s) 650, 1210
PasI CCCWGGG 1 cut(s) 37
PctI GAATGC 1 cut(s) 731
PdmI GAANNNNTTC 2 cut(s) 546, 607
PfeI GAWTC 5 cut(s) 173, 223, 562, 682, 860
PkrI GCNGC 3 cut(s) 166, 964, 1141
PleI GAGTC 1 cut(s) 1241
PpsI GAGTC 1 cut(s) 1241
PpuMI RGGWCCY 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 41
Psp6I CCWGG 6 cut(s) 36, 50, 364, 496, 1098, 1201
PspGI CCWGG 6 cut(s) 36, 50, 364, 496, 1098, 1201
PspN4I GGNNCC 7 cut(s) 42, 60, 124, 216, 394, 1077, 1314
PspPI GGNCC 6 cut(s) 41, 100, 162, 215, 433, 1341
PspPPI RGGWCCY 1 cut(s) 41
PsuI RGATCY 4 cut(s) 511, 613, 780, 1470
RsaI GTAC 4 cut(s) 134, 394, 1174, 1196
RsaNI GTAC 4 cut(s) 133, 393, 1173, 1195
RseI CAYNNNNRTG 1 cut(s) 1446
SaqAI TTAA 7 cut(s) 489, 524, 947, 1116, 1157, 1239, 1322
SatI GCNGC 3 cut(s) 165, 963, 1140
Sau96I GGNCC 6 cut(s) 41, 100, 162, 215, 433, 1341
ScaI AGTACT 1 cut(s) 1174
SchI GAGTC 1 cut(s) 1241
ScrFI CCNGG 7 cut(s) 38, 52, 366, 498, 1073, 1100, 1203
SduI GDGCHC 4 cut(s) 35, 1068, 1080, 1088
SfaNI GCATC 6 cut(s) 56, 434, 718, 1414, 1450, 1466
SfcI CTRYAG 1 cut(s) 298
SfuI TTCGAA 2 cut(s) 226, 601
SinI GGWCC 2 cut(s) 41, 215
SmiMI CAYNNNNRTG 1 cut(s) 1446
SmlI CTYRAG 3 cut(s) 305, 551, 1321
SmoI CTYRAG 3 cut(s) 305, 551, 1321
SpeI ACTAGT 1 cut(s) 1183
SphI GCATGC 2 cut(s) 650, 1210
Sse9I AATT 6 cut(s) 183, 721, 765, 771, 1146, 1296
SsiI CCGC 3 cut(s) 103, 165, 1139
SspMI CTAG 6 cut(s) 617, 888, 923, 1013, 1184, 1338
StyD4I CCNGG 7 cut(s) 36, 50, 364, 496, 1071, 1098, 1201
StyI CCWWGG 6 cut(s) 118, 168, 210, 637, 823, 996
TaaI ACNGT 5 cut(s) 381, 938, 1218, 1410, 1480
TaiI ACGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 69, 204, 226, 601
TasI AATT 6 cut(s) 183, 721, 765, 771, 1146, 1296
TatI WGTACW 2 cut(s) 1172, 1194
TauI GCSGC 2 cut(s) 167, 1142
TfiI GAWTC 5 cut(s) 173, 223, 562, 682, 860
Tru1I TTAA 7 cut(s) 489, 524, 947, 1116, 1157, 1239, 1322
Tru9I TTAA 7 cut(s) 489, 524, 947, 1116, 1157, 1239, 1322
TscAI CASTG 2 cut(s) 993, 1273
TseFI GTSAC 2 cut(s) 419, 838
TseI GCWGC 1 cut(s) 962
Tsp45I GTSAC 2 cut(s) 419, 838
TspDTI ATGAA 4 cut(s) 107, 330, 724, 1287
TspGWI ACGGA 2 cut(s) 71, 1292
TspRI CASTG 2 cut(s) 993, 1273
Vha464I CTTAAG 1 cut(s) 1321
VneI GTGCAC 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 41, 215
XapI RAATTY 3 cut(s) 183, 721, 765
XbaI TCTAGA 1 cut(s) 922
XceI RCATGY 3 cut(s) 650, 1210, 1394
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmnI GAANNNNTTC 2 cut(s) 546, 607
XspI CTAG 6 cut(s) 617, 888, 923, 1013, 1184, 1338
ZrmI AGTACT 1 cut(s) 1174
Zsp2I ATGCAT 2 cut(s) 733, 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.