Rorug05G0488500

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004326
Physical Location & Seq
Forward (+)
67221148 .. 67224549
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0488500.1

Sequence Viewer

Length: 1455 bp
ATGTCTGGTAAGCAACCAATGACCATTCTTACTGATCAGTCTGCTGCCATGGCTAGAGCAATAAAAGAAGTTTTTCCACAAGCTACTCATCGCTTATGTGTGTGGCATCTTTATCAAAATGCTGCTAAGAATCTCAGTCATGTCTTTCATGGATCTTCAGAATTTGTTCATGATTTTAGCAAATGTATGTATGACTATGAGTTTGAGCATGAGTGGCTATTAGCATGGAATGGACTGCTTGAGAAATATAGCCTCAAGGAAAACAAGTGGTTGAGTAATTTATTTGAATTGCGAGAAAAATGGGCACTAGTGTATGGGAGACATACTTTCACTGCTGATATGAAAAGCACACAACGAAGTGAATGCATGAATAATGTGCTTAAGAAATATTTGAAGCCAGAGCATAATCTTTTGCGGTTTTTCGAACATTATGAAAGAGTTTTGGCTGATCGAAGATATCAAGAGTTGATTGCTGAATATAAGATGATACAAGCAACTCCTATCTTAGCAGTAAGTGCACATATGCTGCAACATGGAGCAGAGGTCTATACTCCAGAGGTTTTCTTGCTCTTTCAGAAAGAATTTGTGAGAATTCATAATTATAGTACTTACAAGGTCGGTAAGTCTTTGATGACATCAGAGTATATAGTATCATATAGTTCTGAACATAATGAGCACCTGGTCAAATATGAAGCTTCAGCACAAAATGTTAATTGTAGTTGTAAAAAGTTTACTTTTGTTGGGATTTTGTGTACTCATGCACTAAAAGTGCTTGACAAGAAGAATGTGAAGAAGATTCCATCGCAATACATTTCAAAGCGATGGACAAGAGAAGCAAAAATTATGAGTATTACTAGTTATCATCCTACTGAGATTCACGGTAATCCTAAGGAGTCGATTGGCAAACGGTATGGCTACTTGTGCCGCACTTCTCGTGATATTTTAACTGTTGCAGCAGATGATGAAGAGTTAACGAGATATGCACATGAATGTTTGGTTGAGATGCTCAAAGGCTTAGAGTTAATAAAGAAAAATCGCACTGAGAAAGAAGGGATAAGTTCTAGTGATAGCAGCGAAGTGAATACTACTTTTGGTCAAAGTGAAGAGGTTGAGGGAATAGTTCAATCTAATGCTTATGAAACAACAATTGTCCGTGGAGTTAAAAGAAAAGCTACAATTGGCCGTCCACGTACCAGATTCAAAGACCCGACTGAAAAAAAAAAGAGTAAGGTACCAAAGAAAAGCACAAAAGCTCCCACAAGAAGGAAAATACCCATGCAGGAATTTGAGAGCATTTTGGTCAATTCAGAAACACACCAAATTAGTGAACATGATGTTCAACTATCTGGTGTTTTGACTCAAGTAAGAACATCTTCATATTATAACATTATAATTTTCATGTCTTATTGTATGTCATTTGTGAGCATCTTTATAGTATATCATGATAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

484

Amino Acids

56.33

Weight (kDa)

9.11

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MULE PF10551 1 - 40 2.2e-10 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1383, 1391
Acc65I GGTACC 1 cut(s) 1231
AccB1I GGYRCC 1 cut(s) 1231
AciI CCGC 2 cut(s) 415, 925
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 1 cut(s) 1180
AcsI RAATTY 4 cut(s) 161, 581, 591, 1283
AcuI CTGAAG 2 cut(s) 141, 681
AfaI GTAC 4 cut(s) 607, 754, 1192, 1233
AfiI CCNNNNNNNGG 1 cut(s) 1263
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 6 cut(s) 287, 394, 816, 1124, 1201, 1340
AhlI ACTAGT 2 cut(s) 307, 854
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 4 cut(s) 83, 695, 1172, 1253
AluI AGCT 4 cut(s) 83, 695, 1172, 1253
Alw21I GWGCWC 2 cut(s) 520, 678
Alw26I GTCTC 1 cut(s) 313
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 1 cut(s) 160
AoxI GGCC 1 cut(s) 1180
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 5 cut(s) 44, 122, 526, 953, 1071
ApoI RAATTY 4 cut(s) 161, 581, 591, 1283
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
Asp700I GAANNNNTTC 3 cut(s) 72, 165, 1372
Asp718I GGTACC 1 cut(s) 1231
AsuII TTCGAA 1 cut(s) 423
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 2 cut(s) 307, 520
BanI GGYRCC 1 cut(s) 1231
BauI CACGAG 1 cut(s) 933
Bbv12I GWGCWC 2 cut(s) 520, 678
BbvI GCAGC 5 cut(s) 31, 109, 513, 965, 1083
BccI CCATC 2 cut(s) 808, 816
BceAI ACGGC 1 cut(s) 1167
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BciT130I CCWGG 1 cut(s) 680
BclI TGATCA 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 313
BcuI ACTAGT 2 cut(s) 307, 854
BfaI CTAG 4 cut(s) 54, 308, 855, 1062
BfrI CTTAAG 1 cut(s) 380
BisI GCNGC 6 cut(s) 45, 123, 527, 925, 954, 1072
BlsI GCNGC 6 cut(s) 46, 124, 528, 926, 955, 1073
BmcAI AGTACT 1 cut(s) 607
Bme1390I CCNGG 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 1233
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 3 cut(s) 115, 993, 1434
BpmI CTGGAG 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 423
BpuEI CTTGAG 3 cut(s) 239, 260, 1344
BsaAI YACGTR 1 cut(s) 1190
BsaJI CCNNGG 2 cut(s) 48, 1153
BsaXI ACNNNNNCTCC 4 cut(s) 528, 558, 1237, 1267
Bsc4I CCNNNNNNNGG 1 cut(s) 1263
Bse21I CCTNAGG 1 cut(s) 888
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 2 cut(s) 48, 1153
BseGI GGATG 1 cut(s) 862
BseLI CCNNNNNNNGG 1 cut(s) 1263
BseMII CTCAG 3 cut(s) 148, 861, 1032
BseSI GKGCMC 2 cut(s) 307, 520
BseXI GCAGC 5 cut(s) 31, 109, 513, 965, 1083
BshFI GGCC 1 cut(s) 1182
BshNI GGYRCC 1 cut(s) 1231
BsiHKAI GWGCWC 2 cut(s) 520, 678
BslI CCNNNNNNNGG 1 cut(s) 1263
BsmAI GTCTC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 1 cut(s) 1182
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 3 cut(s) 307, 520, 678
Bsp143I GATC 3 cut(s) 34, 152, 448
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 2 cut(s) 415, 925
BspANI GGCC 1 cut(s) 1182
BspCNI CTCAG 3 cut(s) 147, 862, 1033
BspHI TCATGA 2 cut(s) 169, 1441
BspLI GGNNCC 1 cut(s) 1233
BspPI GGATC 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 1231
BspTI CTTAAG 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 48, 1153
BssMI GATC 3 cut(s) 34, 152, 448
BssSI CACGAG 1 cut(s) 933
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 933
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 881, 909, 949
Bst6I CTCTTC 2 cut(s) 960, 1098
BstAFI CTTAAG 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 423
BstDEI CTNAG 7 cut(s) 126, 134, 505, 870, 888, 1015, 1041
BstDSI CCRYGG 2 cut(s) 48, 1153
BstF5I GGATG 1 cut(s) 862
BstKTI GATC 3 cut(s) 37, 155, 451
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 3 cut(s) 34, 152, 448
BstMWI GCNNNNNNNGC 4 cut(s) 50, 214, 515, 921
BstNI CCWGG 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 678
BstSLI GKGCMC 2 cut(s) 307, 520
BstV1I GCAGC 5 cut(s) 31, 109, 513, 965, 1083
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 888
BsuRI GGCC 1 cut(s) 1182
BtgI CCRYGG 2 cut(s) 48, 1153
BtgZI GCGATG 3 cut(s) 74, 786, 835
BtsCI GGATG 1 cut(s) 862
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 330, 1038
CciI TCATGA 2 cut(s) 169, 1441
CsiI ACCWGGT 1 cut(s) 678
Csp6I GTAC 4 cut(s) 606, 753, 1191, 1232
CviQI GTAC 4 cut(s) 606, 753, 1191, 1232
DdeI CTNAG 7 cut(s) 126, 134, 505, 870, 888, 1015, 1041
DpnI GATC 3 cut(s) 36, 154, 450
DpnII GATC 3 cut(s) 34, 152, 448
EaeI YGGCCR 1 cut(s) 1180
Eam1104I CTCTTC 2 cut(s) 960, 1098
EarI CTCTTC 2 cut(s) 960, 1098
Eco130I CCWWGG 1 cut(s) 48
Eco32I GATATC 1 cut(s) 458
Eco57I CTGAAG 2 cut(s) 141, 681
Eco81I CCTNAGG 1 cut(s) 888
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 678
EcoRV GATATC 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 48
FalI AAGNNNNNCTT 2 cut(s) 1357, 1389
FauNDI CATATG 1 cut(s) 522
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 6 cut(s) 45, 123, 527, 925, 954, 1072
FokI GGATG 1 cut(s) 849
Fsp4HI GCNGC 6 cut(s) 45, 123, 527, 925, 954, 1072
FspBI CTAG 4 cut(s) 54, 308, 855, 1062
GluI GCNGC 6 cut(s) 45, 123, 527, 925, 954, 1072
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 1 cut(s) 1182
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 6 cut(s) 130, 796, 874, 893, 1197, 1357
HpaI GTTAAC 1 cut(s) 972
Hpy166II GTNNAC 6 cut(s) 518, 732, 753, 972, 1187, 1328
Hpy188I TCNGA 5 cut(s) 160, 576, 640, 664, 1309
Hpy188III TCNNGA 5 cut(s) 170, 461, 554, 935, 1442
Hpy8I GTNNAC 6 cut(s) 518, 732, 753, 972, 1187, 1328
HpyAV CCTTC 2 cut(s) 1043, 1257
HpyCH4III ACNGT 3 cut(s) 881, 909, 949
HpyCH4IV ACGT 1 cut(s) 1189
HpyCH4V TGCA 7 cut(s) 366, 518, 529, 761, 953, 983, 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 214, 515, 921
HpyF3I CTNAG 7 cut(s) 126, 134, 505, 870, 888, 1015, 1041
HpySE526I ACGT 1 cut(s) 1189
KpnI GGTACC 1 cut(s) 1235
Ksp22I TGATCA 1 cut(s) 34
KspAI GTTAAC 1 cut(s) 972
Kzo9I GATC 3 cut(s) 34, 152, 448
LmnI GCTCC 2 cut(s) 536, 1258
LpnPI CCDG 7 cut(s) 411, 567, 665, 692, 1207, 1265, 1332
Lsp1109I GCAGC 5 cut(s) 31, 109, 513, 965, 1083
LweI GCATC 3 cut(s) 115, 993, 1434
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 4 cut(s) 54, 308, 855, 1062
MaeII ACGT 1 cut(s) 1189
MalI GATC 3 cut(s) 36, 154, 450
MboI GATC 3 cut(s) 34, 152, 448
MboII GAAGA 8 cut(s) 147, 465, 793, 802, 805, 977, 1115, 1365
MfeI CAATTG 2 cut(s) 1146, 1176
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 3 cut(s) 307, 520, 678
MlyI GAGTC 2 cut(s) 902, 1351
MnlI CCTC 5 cut(s) 263, 535, 550, 1099, 1105
Mph1103I ATGCAT 1 cut(s) 368
MroXI GAANNNNTTC 3 cut(s) 72, 165, 1372
MseI TTAA 6 cut(s) 381, 711, 944, 971, 1022, 1161
MslI CAYNNNNRTG 1 cut(s) 988
MspCI CTTAAG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 680
MunI CAATTG 2 cut(s) 1146, 1176
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 1 cut(s) 680
MwoI GCNNNNNNNGC 4 cut(s) 50, 214, 515, 921
NcoI CCATGG 1 cut(s) 48
NdeI CATATG 1 cut(s) 522
NdeII GATC 3 cut(s) 34, 152, 448
NlaIV GGNNCC 1 cut(s) 1233
NsiI ATGCAT 1 cut(s) 368
NspV TTCGAA 1 cut(s) 423
PagI TCATGA 2 cut(s) 169, 1441
PctI GAATGC 1 cut(s) 368
PdmI GAANNNNTTC 3 cut(s) 72, 165, 1372
PfeI GAWTC 4 cut(s) 130, 796, 874, 1197
PkrI GCNGC 6 cut(s) 46, 124, 528, 926, 955, 1073
PleI GAGTC 2 cut(s) 901, 1351
PpsI GAGTC 2 cut(s) 901, 1351
Ppu21I YACGTR 1 cut(s) 1190
PsiI TTATAA 2 cut(s) 1383, 1391
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 1 cut(s) 1233
PsuI RGATCY 1 cut(s) 152
RsaI GTAC 4 cut(s) 607, 754, 1192, 1233
RsaNI GTAC 4 cut(s) 606, 753, 1191, 1232
RseI CAYNNNNRTG 1 cut(s) 988
SaqAI TTAA 6 cut(s) 381, 711, 944, 971, 1022, 1161
SatI GCNGC 6 cut(s) 45, 123, 527, 925, 954, 1072
Sau3AI GATC 3 cut(s) 34, 152, 448
ScaI AGTACT 1 cut(s) 607
SchI GAGTC 2 cut(s) 902, 1351
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 3 cut(s) 307, 520, 678
SexAI ACCWGGT 1 cut(s) 678
SfaNI GCATC 3 cut(s) 115, 993, 1434
SfuI TTCGAA 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 988
SmlI CTYRAG 4 cut(s) 239, 254, 380, 1359
SmoI CTYRAG 4 cut(s) 239, 254, 380, 1359
SpeI ACTAGT 2 cut(s) 307, 854
SsiI CCGC 2 cut(s) 415, 925
SspI AATATT 1 cut(s) 389
SspMI CTAG 4 cut(s) 54, 308, 855, 1062
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 881, 909, 949
TaiI ACGT 1 cut(s) 1192
TaqI TCGA 3 cut(s) 423, 451, 896
TatI WGTACW 2 cut(s) 605, 752
TauI GCSGC 1 cut(s) 927
TfiI GAWTC 4 cut(s) 130, 796, 874, 1197
Tru1I TTAA 6 cut(s) 381, 711, 944, 971, 1022, 1161
Tru9I TTAA 6 cut(s) 381, 711, 944, 971, 1022, 1161
TscAI CASTG 2 cut(s) 337, 1045
TseI GCWGC 5 cut(s) 44, 122, 526, 953, 1071
TspGWI ACGGA 1 cut(s) 1142
TspRI CASTG 2 cut(s) 337, 1045
Vha464I CTTAAG 1 cut(s) 380
VneI GTGCAC 1 cut(s) 516
XapI RAATTY 4 cut(s) 161, 581, 591, 1283
XmnI GAANNNNTTC 3 cut(s) 72, 165, 1372
XspI CTAG 4 cut(s) 54, 308, 855, 1062
ZrmI AGTACT 1 cut(s) 607
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.