pycom03g00160

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004326
Physical Location & Seq
Forward (+)
163542 .. 164657
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00160.1

Sequence Viewer

Length: 1116 bp
ATGGACGAAGCAATGGTAGCAAGGTATTGGTGTCATAGGTGTTCTCAAATGGTGAATCCCATCATGGAAGTAGAAATGAAATGCCCCTTTTGCCAAAGCGGATTTATTGAAGAAATCAATGGCACTACAAGGGAGAATCAAGACGCTGACTCTGATTTGGGTTCCGATCGTGCCCTCTCCCTCTGGGCTCCAATCCTGTTGGGCATGATGAACAATCCCCGTCGTCGCCGGAGATTAAGACGACTCGACTTTGATGACGATGATGATGATGACAATGACAATGACAATGATGATGGTGACACTCGCCAGGGCGGAGATACTGAATTGGACAGAGAACTTGAATCAATCATCCGGAGGAGGAGAAGAAGCTCAGCTACTATTCTACAGCTGCTTCAAGGCATTCGAGCTGGATTGGCATCTGAGTCTGAGAATTCTGAGGGGGATAGGGAAAGAGAAAGGGAGAGGGAGCGTGTAATTCTTATAAATCCTTTCAATCAAACCATCATTGTTCAGGGATCTTACGATTCGAATCAGGGTCAAAACAACAATCACACCCCTATTGGTTCATTAGGCGATTATTTCATCGGGCCTGGTTTAGATTTGTTGCTGCAACATTTGGCAGAGAATGATCCTAATAGATATGGGACTCCACCTGCGCAAAAGGAGGCAGTGGAAGCTCTGCCCACTGTGACGATCAAGGAGAATTTGCAGTGTTCAGTGTGCTTGGATGATTTTGAGGTCGGTGTAGAAGCAAAAGAGATGCCATGTAAGCACAAGTTTCATAGCGGGTGTATTCTGCCATGGCTGGAGCTTCATAGTTCCTGTCCAGTTTGCAGGTTCCAGTTGCCTGCTGATGAATCAAAGCGTGATTCAGACATTTCCAGGAATAGCAGTAACTCGAGGGAGAGTGAAGAGATTGGTGATGATGTTGGTGGGGAAGAGGGAGATGAGGATGGGAGAAACGGAAACGGGAGAAGGTTTTCAATACCGTGGCCTTTCAATGGTCTGTTTTCTCACTCAGGATCTCAGTCAGGTGGCAGTGTGGGAAATTCATCATCATCATCGTCGGCAAATGCAACCGGAAGTGGAAGCACTTCTCAAACAGAAGAGAACTGA

Protein Analysis

372

Amino Acids

41.24

Weight (kDa)

4.6

Isoelectric Point (pI)

60.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 8 - 39 9e-12 zinc-ribbon
zf-RING_2 PF13639 237 - 279 6.9e-14 Ring finger domain
zf-C3HC4_2 PF13923 237 - 278 5e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 237 - 265 6.2e-09 RING-like zinc finger
zf-C3HC4 PF00097 238 - 278 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 251 - 279 1.5e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AarI CACCTGC 1 cut(s) 661
Acc16I TGCGCA 1 cut(s) 657
Acc36I ACCTGC 2 cut(s) 661, 825
AccIII TCCGGA 1 cut(s) 351
AciI CCGC 3 cut(s) 99, 312, 786
AclWI GGATC 3 cut(s) 523, 623, 1030
AcsI RAATTY 3 cut(s) 430, 703, 1048
AfiI CCNNNNNNNGG 1 cut(s) 1001
AgsI TTSAA 6 cut(s) 110, 341, 395, 493, 984, 1000
AjnI CCWGG 3 cut(s) 306, 589, 881
AluBI AGCT 6 cut(s) 369, 374, 388, 407, 677, 811
AluI AGCT 6 cut(s) 369, 374, 388, 407, 677, 811
AlwI GGATC 3 cut(s) 523, 623, 1030
Ama87I CYCGRG 1 cut(s) 898
Aor13HI TCCGGA 1 cut(s) 351
AoxI GGCC 2 cut(s) 587, 992
ApeKI GCWGC 2 cut(s) 388, 607
ApoI RAATTY 3 cut(s) 430, 703, 1048
Asp700I GAANNNNTTC 2 cut(s) 979, 1093
AspLEI GCGC 1 cut(s) 658
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 3 cut(s) 64, 308, 932
AsuII TTCGAA 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 898
BaeGI GKGCMC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 190
BarI GAAGNNNNNNTAC 2 cut(s) 358, 390
BbvI GCAGC 2 cut(s) 375, 594
BccI CCATC 4 cut(s) 68, 287, 509, 947
BciT130I CCWGG 3 cut(s) 308, 591, 883
BfmI CTRYAG 1 cut(s) 383
BfuAI ACCTGC 2 cut(s) 661, 825
BisI GCNGC 2 cut(s) 389, 608
BlpI GCTNAGC 1 cut(s) 370
BlsI GCNGC 2 cut(s) 390, 609
Bme1390I CCNGG 3 cut(s) 308, 591, 883
BmeT110I CYCGRG 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 3 cut(s) 163, 189, 839
BmrFI CCNGG 3 cut(s) 308, 591, 883
BmsI GCATC 2 cut(s) 425, 750
BpmI CTGGAG 1 cut(s) 827
Bpu1102I GCTNAGC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 527
BsaBI GATNNNNATC 2 cut(s) 415, 528
BsaJI CCNNGG 3 cut(s) 307, 800, 989
BsaWI WCCGGW 2 cut(s) 351, 1079
Bsc4I CCNNNNNNNGG 1 cut(s) 1001
Bse1I ACTGG 2 cut(s) 827, 841
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 2 cut(s) 415, 528
BseAI TCCGGA 1 cut(s) 351
BseBI CCWGG 3 cut(s) 308, 591, 883
BseDI CCNNGG 3 cut(s) 307, 800, 989
BseGI GGATG 3 cut(s) 348, 733, 958
BseJI GATNNNNATC 2 cut(s) 415, 528
BseLI CCNNNNNNNGG 1 cut(s) 1001
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 6 cut(s) 384, 411, 417, 426, 1032, 1040
BseNI ACTGG 2 cut(s) 827, 841
BseRI GAGGAG 2 cut(s) 370, 373
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 2 cut(s) 375, 594
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 589, 994
BsiEI CGRYCG 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 898
BsiSI CCGG 3 cut(s) 229, 352, 1080
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 2 cut(s) 589, 994
BsoBI CYCGRG 1 cut(s) 898
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 175, 190
Bsp13I TCCGGA 1 cut(s) 351
Bsp143I GATC 5 cut(s) 166, 515, 628, 693, 1022
Bsp1720I GCTNAGC 1 cut(s) 370
Bsp19I CCATGG 1 cut(s) 800
BspACI CCGC 3 cut(s) 99, 312, 786
BspANI GGCC 2 cut(s) 589, 994
BspCNI CTCAG 6 cut(s) 383, 412, 418, 427, 1031, 1039
BspEI TCCGGA 1 cut(s) 351
BspLI GGNNCC 3 cut(s) 163, 189, 839
BspMI ACCTGC 2 cut(s) 661, 825
BspPI GGATC 3 cut(s) 523, 623, 1030
BspT104I TTCGAA 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 827, 841
BssECI CCNNGG 3 cut(s) 307, 800, 989
BssMI GATC 5 cut(s) 166, 515, 628, 693, 1022
BssT1I CCWWGG 1 cut(s) 800
Bst2UI CCWGG 3 cut(s) 308, 591, 883
Bst4CI ACNGT 2 cut(s) 688, 990
Bst6I CTCTTC 3 cut(s) 906, 933, 1101
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 6 cut(s) 370, 420, 426, 435, 1018, 1026
BstDSI CCRYGG 2 cut(s) 800, 989
BstF5I GGATG 3 cut(s) 348, 733, 958
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 5 cut(s) 169, 518, 631, 696, 1025
BstMBI GATC 5 cut(s) 166, 515, 628, 693, 1022
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 5 cut(s) 17, 90, 413, 674, 769
BstNI CCWGG 3 cut(s) 308, 591, 883
BstSCI CCNGG 3 cut(s) 306, 589, 881
BstSFI CTRYAG 1 cut(s) 383
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 375, 594
BstX2I RGATCY 2 cut(s) 515, 1022
BstYI RGATCY 2 cut(s) 515, 1022
BsuRI GGCC 2 cut(s) 589, 994
BtgI CCRYGG 2 cut(s) 800, 989
BtsCI GGATG 3 cut(s) 348, 733, 958
BtsI GCAGTG 3 cut(s) 675, 716, 1045
BtsIMutI CAGTG 5 cut(s) 675, 684, 716, 723, 1045
BveI ACCTGC 2 cut(s) 661, 825
Cac8I GCNNGC 1 cut(s) 849
CfoI GCGC 1 cut(s) 658
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 1 cut(s) 152
CviAII CATG 4 cut(s) 64, 205, 765, 801
DdeI CTNAG 6 cut(s) 370, 420, 426, 435, 1018, 1026
DpnI GATC 5 cut(s) 168, 517, 630, 695, 1024
DpnII GATC 5 cut(s) 166, 515, 628, 693, 1022
Eam1104I CTCTTC 3 cut(s) 906, 933, 1101
EarI CTCTTC 3 cut(s) 906, 933, 1101
EciI GGCGGA 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 800
Eco24I GRGCYC 1 cut(s) 190
Eco88I CYCGRG 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 306, 589, 881
EcoT14I CCWWGG 1 cut(s) 800
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 800
FaeI CATG 4 cut(s) 67, 208, 768, 804
FaiI YATR 9 cut(s) 36, 65, 206, 482, 642, 766, 783, 802, 816
FaqI GGGAC 1 cut(s) 658
FatI CATG 4 cut(s) 63, 204, 764, 800
FauI CCCGC 1 cut(s) 779
Fnu4HI GCNGC 2 cut(s) 389, 608
FokI GGATG 3 cut(s) 335, 740, 965
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 2 cut(s) 389, 608
FspI TGCGCA 1 cut(s) 657
GlaI GCGC 1 cut(s) 657
GluI GCNGC 2 cut(s) 389, 608
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 2 cut(s) 589, 994
HapII CCGG 3 cut(s) 229, 352, 1080
HgaI GACGC 1 cut(s) 152
HhaI GCGC 1 cut(s) 658
Hin1II CATG 4 cut(s) 67, 208, 768, 804
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HpaII CCGG 3 cut(s) 229, 352, 1080
HphI GGTGA 3 cut(s) 64, 308, 932
Hpy188I TCNGA 6 cut(s) 154, 166, 421, 427, 436, 874
Hpy188III TCNNGA 3 cut(s) 140, 352, 1020
Hpy99I CGWCG 3 cut(s) 225, 228, 1069
HpyAV CCTTC 1 cut(s) 969
HpyCH4III ACNGT 2 cut(s) 688, 990
HpyCH4V TGCA 4 cut(s) 610, 709, 834, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 90, 413, 674, 769
HpyF3I CTNAG 6 cut(s) 370, 420, 426, 435, 1018, 1026
Hsp92II CATG 4 cut(s) 67, 208, 768, 804
HspAI GCGC 1 cut(s) 656
Kpn2I TCCGGA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 166, 515, 628, 693, 1022
LmnI GCTCC 3 cut(s) 193, 466, 808
Lsp1109I GCAGC 2 cut(s) 375, 594
LweI GCATC 2 cut(s) 425, 750
MaeIII GTNAC 3 cut(s) 296, 688, 893
MalI GATC 5 cut(s) 168, 517, 630, 695, 1024
MboI GATC 5 cut(s) 166, 515, 628, 693, 1022
MboII GAAGA 4 cut(s) 122, 375, 923, 950
MflI RGATCY 2 cut(s) 515, 1022
MhlI GDGCHC 2 cut(s) 175, 190
MluCI AATT 5 cut(s) 323, 430, 474, 703, 1048
MlyI GAGTC 4 cut(s) 143, 237, 431, 640
MroI TCCGGA 1 cut(s) 351
MroXI GAANNNNTTC 2 cut(s) 979, 1093
MseI TTAA 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 3 cut(s) 229, 352, 1080
MspR9I CCNGG 3 cut(s) 308, 591, 883
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 3 cut(s) 308, 591, 883
MwoI GCNNNNNNNGC 5 cut(s) 17, 90, 413, 674, 769
NcoI CCATGG 1 cut(s) 800
NdeII GATC 5 cut(s) 166, 515, 628, 693, 1022
NlaIII CATG 4 cut(s) 67, 208, 768, 804
NlaIV GGNNCC 3 cut(s) 163, 189, 839
NmuCI GTSAC 2 cut(s) 296, 688
NsbI TGCGCA 1 cut(s) 657
NspV TTCGAA 1 cut(s) 527
PaeR7I CTCGAG 1 cut(s) 898
PaqCI CACCTGC 1 cut(s) 661
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 2 cut(s) 979, 1093
PfeI GAWTC 7 cut(s) 55, 136, 341, 524, 529, 857, 869
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 2 cut(s) 390, 609
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 4 cut(s) 143, 237, 430, 640
PpsI GAGTC 4 cut(s) 143, 237, 430, 640
PsiI TTATAA 1 cut(s) 482
Psp6I CCWGG 3 cut(s) 306, 589, 881
PspGI CCWGG 3 cut(s) 306, 589, 881
PspN4I GGNNCC 3 cut(s) 163, 189, 839
PspPI GGNCC 1 cut(s) 587
PspXI VCTCGAGB 1 cut(s) 898
PsuI RGATCY 2 cut(s) 515, 1022
PvuI CGATCG 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 388
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 2 cut(s) 389, 608
Sau3AI GATC 5 cut(s) 166, 515, 628, 693, 1022
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 4 cut(s) 143, 237, 431, 640
ScrFI CCNGG 3 cut(s) 308, 591, 883
SduI GDGCHC 2 cut(s) 175, 190
SfaNI GCATC 2 cut(s) 425, 750
SfcI CTRYAG 1 cut(s) 383
Sfr274I CTCGAG 1 cut(s) 898
SfuI TTCGAA 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 898
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
Sse9I AATT 5 cut(s) 323, 430, 474, 703, 1048
SsiI CCGC 3 cut(s) 99, 312, 786
StyD4I CCNGG 3 cut(s) 306, 589, 881
StyI CCWWGG 1 cut(s) 800
TaaI ACNGT 2 cut(s) 688, 990
TaqI TCGA 4 cut(s) 246, 403, 527, 899
TasI AATT 5 cut(s) 323, 430, 474, 703, 1048
TfiI GAWTC 7 cut(s) 55, 136, 341, 524, 529, 857, 869
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 5 cut(s) 675, 691, 716, 723, 1045
TseFI GTSAC 2 cut(s) 296, 688
TseI GCWGC 2 cut(s) 388, 607
Tsp45I GTSAC 2 cut(s) 296, 688
TspDTI ATGAA 8 cut(s) 92, 224, 555, 571, 770, 803, 870, 1041
TspGWI ACGGA 1 cut(s) 978
TspRI CASTG 5 cut(s) 675, 691, 716, 723, 1045
XapI RAATTY 3 cut(s) 430, 703, 1048
XhoI CTCGAG 1 cut(s) 898
XmnI GAANNNNTTC 2 cut(s) 979, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.