MD03G1005400.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015924
Physical Location & Seq
Reverse (-)
443688 .. 445370
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1005400.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGAAAGAATCGAATCCGACCACGGAGCCCATCGGGCAAAACATCATCAAACTCATCAGCAATCTCTGTTTCTCAGTTTTTGTATTTTCGGTGCTTATAATTACTGTAATTGCCATCACCTACCAACCCCCAGATCCATGGCTGGAGTCAGCTCCGGCCTTAACGAAACTCTTCACCGCTTCCGAGAACGCCACTTTCAAAAATGATGACTCTGTCCTCAAAACTGGCGAGGATTTACCTCTTTCCCCCGTCGTTCCCCCGGCTTCTTCGGGTTTTGCAATCACGGAGGCTGTGATTGCGAAAGCCGAAGCCGAGGTCTCCAGTCCGACCAATTCGACCCTGTCGGACGGGTTGAATTGTGAGGATTTGGAGAGAGTGAATTGCTCTGACCCACGGGCTTTGATTGCGGTGGAGAGATTTAATTTGAGGTTGTTTAAGAACATTGTGTTCTTGGAGTATCAGACGCCGGTTAACGGGTCGAAAGAGGATGAATGTGACGTGTTGTGGAAATTTAGGAACAAAAAGGAGAAGTCTTGGAGGAAGTATAGGGATTTTAGGAGGTTTAAGCTCGGATTCGGAGTGAATTGTACTTATAAGGTGGTGCACGCCAGAGGGTGGCATTCAGGTATTAATGCGAGGGGGGCGAGGAGGATTAGTAGTAATGGCACCAGGGGTGGTGGGAATGCTAGAATCGCCCCACCAGTACGTGATGAACAGATCAATGATACAATCCCAAGCTTGGGATCGGATAGGAATTTTAGGAGGGGGAGGTACTTGTACTATTCGCGTGGAGGGGATTATTGCAAGGGGATGAATCAGTTCCAATGGAGTTTCTTGTGTGGTTTAGGTGAGGCTATGTATTTGAACAGGACGTTTGTGATGGATTTGAGTGTCTGCTTGGCGGGGAGTTATACTGCAAGTAATAAAGATGAGGAGGAGAAAGATTTTCGGTACTACTTTGATTATGAACATCTGAAGGAAACTGCCTCAATTGTGGATGAGGTTGAATTTCTGCAAGATTGGAAGAAGTGGGATCGGAGCCATAAGAGGAAAGTGCCCGTTAAGAAGGTTGCGAGCCATAAGGTGACGCCAATGCAGCTGAAGAAAGAGAGGAGCACGATTATATGGAGGCAGTTTGATGGTCCGGAGCCAGAGAACTACTGGTACAGGGTGTGTGAAGGACAGGCTGCTAAGTACGTTCAGAGGCCGTGGCATGCCCTGTGGAAATCAAAGAGATTGATGAATATTGTTACAGAGATAAGTGGGAAAATGGACTGGGATTTTGATGCTGTTCATGTTGTGCGAGGAGAGAAGGCACAGAATAAGGAGCTTTGGCCTCACCTCGATTATGATACATCCCCCGACATGCTTCTTGAGAAGGTTAAGACGATGGTGCAGCCTTGGAGGAATCTGTATATAGCCACCAATGAGCCGTTCTACAATTACTTCGATAAACTGAGGTCACAGTACAAGGTTCATTTGCTTGATGACTACAAAGAACTTTGGAGTAACACAAGTGAATGGTACAATGAGACTAGCATTTTAAACAACGGACGCCCAGTTGACTTCGATGGATATATGAGGGTTGAAGTGGATACCGAGGTTCTTTACAGGGCAAAGACGCGTGTGGAAACATTCTATAATTTGACGGCAGATTGCAAGGACGGAATCAACACATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

561

Amino Acids

64.89

Weight (kDa)

8.12

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7074 PF23269 125 - 208 3.9e-35 Domain of unknown function (DUF7074)
DUF7075 PF23272 252 - 545 2.2e-59 Domain of unknown function (DUF7075)
O-FucT PF10250 258 - 493 7.4e-06 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08810
fragaria_vesca FvH4_3g45390
malus_domestica MD03G1005400.v1.1 MD11G1008000.v1.1
prunus_persica Prupe.6G005200_v2.0.a1
pyrus_communis pycom03g00560
rosa_chinensis RchiOBHm_Chr5g0083201
rosa_laevigata RLG00000037016
rosa_roxburghii Rroxscaffold_1G00001300
rosa_rugosa Rorug05G0483200.1
rosa_samantha Rh5AG532600 Rh5BG559800 Rh5CG582500 Rh5DG569000
rosa_wichuraiana Rw5G050040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 98, 594
AccB1I GGYRCC 1 cut(s) 665
AccB7I CCANNNNNTGG 1 cut(s) 615
AccII CGCG 2 cut(s) 787, 1624
AccIII TCCGGA 1 cut(s) 1144
AciI CCGC 3 cut(s) 177, 407, 902
AclWI GGATC 3 cut(s) 128, 751, 1041
AcsI RAATTY 3 cut(s) 509, 754, 1007
AcuI CTGAAG 2 cut(s) 995, 1121
AcyI GRCGYC 3 cut(s) 464, 1088, 1555
AfaI GTAC 9 cut(s) 589, 705, 773, 779, 953, 1166, 1196, 1469, 1526
AfiI CCNNNNNNNGG 4 cut(s) 473, 615, 739, 740
AflIII ACRYGT 2 cut(s) 498, 1622
AgsI TTSAA 5 cut(s) 199, 355, 865, 1007, 1589
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 1 cut(s) 668
AjuI GAANNNNNNNTTGG 2 cut(s) 1418, 1450
AluBI AGCT 5 cut(s) 152, 568, 738, 1099, 1330
AluI AGCT 5 cut(s) 152, 568, 738, 1099, 1330
Alw21I GWGCWC 2 cut(s) 606, 1118
Alw26I GTCTC 2 cut(s) 322, 1526
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 3 cut(s) 128, 751, 1041
Aor13HI TCCGGA 1 cut(s) 1144
AoxI GGCC 3 cut(s) 156, 1205, 1334
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 3 cut(s) 1096, 1187, 1396
ApoI RAATTY 3 cut(s) 509, 754, 1007
ArsI GACNNNNNNTTYG 4 cut(s) 300, 332, 1265, 1297
AseI ATTAAT 1 cut(s) 630
Asp700I GAANNNNTTC 2 cut(s) 170, 818
AspS9I GGNCC 1 cut(s) 1142
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 5 cut(s) 109, 166, 860, 1096, 1331
AvaII GGWCC 1 cut(s) 1142
BaeGI GKGCMC 2 cut(s) 606, 1059
BaeI ACNNNNGTAYC 2 cut(s) 1587, 1620
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 606, 1118
BbvI GCAGC 3 cut(s) 1108, 1174, 1408
BccI CCATC 6 cut(s) 38, 122, 874, 1133, 1384, 1565
BceAI ACGGC 3 cut(s) 1192, 1417, 1665
BciT130I CCWGG 1 cut(s) 670
BciVI GTATCC 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 2 cut(s) 322, 1526
BfaI CTAG 2 cut(s) 687, 1536
BfuI GTATCC 1 cut(s) 1588
BglI GCCNNNNNGGC 1 cut(s) 34
BisI GCNGC 3 cut(s) 1097, 1188, 1397
BlsI GCNGC 3 cut(s) 1098, 1189, 1398
Bme1390I CCNGG 2 cut(s) 260, 670
Bme18I GGWCC 1 cut(s) 1142
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 1142
BmiI GGNNCC 4 cut(s) 27, 667, 1040, 1149
BmrFI CCNGG 2 cut(s) 260, 670
BmrI ACTGGG 2 cut(s) 1285, 1553
BmsI GCATC 1 cut(s) 1276
BmuI ACTGGG 2 cut(s) 1285, 1553
BpmI CTGGAG 2 cut(s) 164, 304
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 707
BsaHI GRCGYC 3 cut(s) 464, 1088, 1555
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 9 cut(s) 21, 137, 258, 312, 392, 669, 1208, 1400, 1599
BsaWI WCCGGW 1 cut(s) 1144
BsaXI ACNNNNNCTCC 2 cut(s) 640, 670
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 615, 739, 740
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 6 cut(s) 229, 321, 701, 1166, 1280, 1559
BseAI TCCGGA 1 cut(s) 1144
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 9 cut(s) 21, 137, 258, 312, 392, 669, 1208, 1400, 1599
BseGI GGATG 4 cut(s) 493, 816, 1003, 1355
BseLI CCNNNNNNNGG 4 cut(s) 473, 615, 739, 740
BseMII CTCAG 2 cut(s) 87, 1448
BseNI ACTGG 6 cut(s) 229, 321, 701, 1166, 1280, 1559
BseRI GAGGAG 5 cut(s) 661, 947, 950, 1126, 1320
BseSI GKGCMC 2 cut(s) 606, 1059
BseXI GCAGC 3 cut(s) 1108, 1174, 1408
BsgI GTGCAG 1 cut(s) 1415
Bsh1236I CGCG 2 cut(s) 787, 1624
BshFI GGCC 3 cut(s) 158, 1207, 1336
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 2 cut(s) 606, 1118
BsiSI CCGG 4 cut(s) 155, 260, 467, 1145
BslI CCNNNNNNNGG 4 cut(s) 473, 615, 739, 740
BsmAI GTCTC 2 cut(s) 322, 1526
BsmI GAATGC 2 cut(s) 619, 688
BsnI GGCC 3 cut(s) 158, 1207, 1336
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 4 cut(s) 30, 606, 1059, 1118
Bsp13I TCCGGA 1 cut(s) 1144
Bsp143I GATC 4 cut(s) 133, 717, 743, 1033
Bsp19I CCATGG 1 cut(s) 137
BspACI CCGC 3 cut(s) 177, 407, 902
BspANI GGCC 3 cut(s) 158, 1207, 1336
BspCNI CTCAG 2 cut(s) 86, 1449
BspEI TCCGGA 1 cut(s) 1144
BspFNI CGCG 2 cut(s) 787, 1624
BspLI GGNNCC 4 cut(s) 27, 667, 1040, 1149
BspPI GGATC 3 cut(s) 128, 751, 1041
BspT107I GGYRCC 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 6 cut(s) 229, 321, 701, 1166, 1280, 1559
BssAI RCCGGY 1 cut(s) 466
BssECI CCNNGG 9 cut(s) 21, 137, 258, 312, 392, 669, 1208, 1400, 1599
BssMI GATC 4 cut(s) 133, 717, 743, 1033
BssNI GRCGYC 3 cut(s) 464, 1088, 1555
BssT1I CCWWGG 2 cut(s) 137, 1400
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 2 cut(s) 106, 1467
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 3 cut(s) 464, 1088, 1555
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 3 cut(s) 606, 1075, 1215
BstDEI CTNAG 3 cut(s) 73, 1191, 1457
BstDSI CCRYGG 4 cut(s) 21, 137, 392, 1208
BstF5I GGATG 4 cut(s) 493, 816, 1003, 1355
BstFNI CGCG 2 cut(s) 787, 1624
BstKTI GATC 4 cut(s) 136, 720, 746, 1036
BstMAI GTCTC 2 cut(s) 322, 1526
BstMBI GATC 4 cut(s) 133, 717, 743, 1033
BstMWI GCNNNNNNNGC 6 cut(s) 34, 296, 404, 641, 692, 1096
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 3 cut(s) 1217, 1369, 1680
BstSCI CCNGG 2 cut(s) 258, 668
BstSLI GKGCMC 2 cut(s) 606, 1059
BstUI CGCG 2 cut(s) 787, 1624
BstV1I GCAGC 3 cut(s) 1108, 1174, 1408
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 1588
BsuRI GGCC 3 cut(s) 158, 1207, 1336
BtgI CCRYGG 4 cut(s) 21, 137, 392, 1208
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 4 cut(s) 493, 816, 1003, 1355
Cac8I GCNNGC 3 cut(s) 606, 1075, 1215
Cfr10I RCCGGY 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 1142
CseI GACGC 4 cut(s) 472, 1096, 1563, 1630
Csp6I GTAC 9 cut(s) 588, 704, 772, 778, 952, 1165, 1195, 1468, 1525
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviAII CATG 5 cut(s) 138, 1214, 1295, 1366, 1677
CviQI GTAC 9 cut(s) 588, 704, 772, 778, 952, 1165, 1195, 1468, 1525
DdeI CTNAG 3 cut(s) 73, 1191, 1457
DpnI GATC 4 cut(s) 135, 719, 745, 1035
DpnII GATC 4 cut(s) 133, 717, 743, 1033
DraI TTTAAA 1 cut(s) 1545
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 137, 1400
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1142
Eco57I CTGAAG 2 cut(s) 995, 1121
EcoRII CCWGG 1 cut(s) 668
EcoT14I CCWWGG 2 cut(s) 137, 1400
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 137, 1400
FaeI CATG 5 cut(s) 141, 1217, 1298, 1369, 1680
FatI CATG 5 cut(s) 137, 1213, 1294, 1365, 1676
FauI CCCGC 1 cut(s) 895
Fnu4HI GCNGC 3 cut(s) 1097, 1188, 1397
FokI GGATG 4 cut(s) 500, 823, 1010, 1342
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 3 cut(s) 1097, 1188, 1397
FspBI CTAG 2 cut(s) 687, 1536
GluI GCNGC 3 cut(s) 1097, 1188, 1397
GsuI CTGGAG 2 cut(s) 164, 304
HaeIII GGCC 3 cut(s) 158, 1207, 1336
HapII CCGG 4 cut(s) 155, 260, 467, 1145
HgaI GACGC 4 cut(s) 472, 1096, 1563, 1630
Hin1I GRCGYC 3 cut(s) 464, 1088, 1555
Hin1II CATG 5 cut(s) 141, 1217, 1298, 1369, 1680
HincII GTYRAC 2 cut(s) 472, 1564
HindII GTYRAC 2 cut(s) 472, 1564
HindIII AAGCTT 1 cut(s) 736
HinfI GANTC 9 cut(s) 8, 13, 146, 209, 573, 690, 814, 1408, 1668
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 4 cut(s) 155, 260, 467, 1145
HphI GGTGA 5 cut(s) 109, 166, 860, 1096, 1331
Hpy166II GTNNAC 3 cut(s) 472, 604, 1564
Hpy188III TCNNGA 2 cut(s) 1145, 1373
Hpy8I GTNNAC 3 cut(s) 472, 604, 1564
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 5 cut(s) 970, 1060, 1172, 1306, 1372
HpyCH4III ACNGT 2 cut(s) 106, 1467
HpyCH4IV ACGT 4 cut(s) 498, 706, 872, 1197
HpyCH4V TGCA 8 cut(s) 278, 604, 804, 917, 1015, 1096, 1396, 1659
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 296, 404, 641, 692, 1096
HpyF3I CTNAG 3 cut(s) 73, 1191, 1457
HpySE526I ACGT 4 cut(s) 498, 706, 872, 1197
Hsp92I GRCGYC 3 cut(s) 464, 1088, 1555
Hsp92II CATG 5 cut(s) 141, 1217, 1298, 1369, 1680
Kpn2I TCCGGA 1 cut(s) 1144
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 4 cut(s) 133, 717, 743, 1033
LmnI GCTCC 6 cut(s) 25, 157, 1038, 1113, 1147, 1327
Lsp1109I GCAGC 3 cut(s) 1108, 1174, 1408
LweI GCATC 1 cut(s) 1276
MaeI CTAG 2 cut(s) 687, 1536
MaeII ACGT 4 cut(s) 498, 706, 872, 1197
MaeIII GTNAC 5 cut(s) 494, 1084, 1249, 1461, 1508
MalI GATC 4 cut(s) 135, 719, 745, 1035
MboI GATC 4 cut(s) 133, 717, 743, 1033
MboII GAAGA 4 cut(s) 163, 258, 1036, 1114
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 4 cut(s) 30, 606, 1059, 1118
MluI ACGCGT 1 cut(s) 1622
MlyI GAGTC 2 cut(s) 155, 203
MmeI TCCRAC 3 cut(s) 41, 324, 350
MroI TCCGGA 1 cut(s) 1144
MroXI GAANNNNTTC 2 cut(s) 170, 818
MseI TTAA 9 cut(s) 161, 420, 435, 471, 564, 630, 1062, 1383, 1544
MspA1I CMGCKG 1 cut(s) 1099
MspI CCGG 4 cut(s) 155, 260, 467, 1145
MspR9I CCNGG 2 cut(s) 260, 670
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 2 cut(s) 619, 688
MvaI CCWGG 1 cut(s) 670
MvnI CGCG 2 cut(s) 787, 1624
MwoI GCNNNNNNNGC 6 cut(s) 34, 296, 404, 641, 692, 1096
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 137
NdeII GATC 4 cut(s) 133, 717, 743, 1033
NlaIII CATG 5 cut(s) 141, 1217, 1298, 1369, 1680
NlaIV GGNNCC 4 cut(s) 27, 667, 1040, 1149
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 3 cut(s) 494, 1084, 1461
NspI RCATGY 3 cut(s) 1217, 1369, 1680
PaeI GCATGC 1 cut(s) 1217
PctI GAATGC 2 cut(s) 619, 688
PdmI GAANNNNTTC 2 cut(s) 170, 818
PfeI GAWTC 7 cut(s) 8, 13, 573, 690, 814, 1408, 1668
PflFI GACNNNGTC 2 cut(s) 212, 340
PflMI CCANNNNNTGG 1 cut(s) 615
PkrI GCNGC 3 cut(s) 1098, 1189, 1398
PleI GAGTC 2 cut(s) 154, 203
PpsI GAGTC 2 cut(s) 154, 203
Ppu21I YACGTR 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 630
PsiI TTATAA 2 cut(s) 98, 594
Psp6I CCWGG 1 cut(s) 668
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 4 cut(s) 27, 667, 1040, 1149
PspPI GGNCC 1 cut(s) 1142
PsrI GAACNNNNNNTAC 2 cut(s) 1148, 1180
PsuI RGATCY 1 cut(s) 133
PsyI GACNNNGTC 2 cut(s) 212, 340
PvuII CAGCTG 1 cut(s) 1099
RsaI GTAC 9 cut(s) 589, 705, 773, 779, 953, 1166, 1196, 1469, 1526
RsaNI GTAC 9 cut(s) 588, 704, 772, 778, 952, 1165, 1195, 1468, 1525
SaqAI TTAA 9 cut(s) 161, 420, 435, 471, 564, 630, 1062, 1383, 1544
SatI GCNGC 3 cut(s) 1097, 1188, 1397
Sau3AI GATC 4 cut(s) 133, 717, 743, 1033
Sau96I GGNCC 1 cut(s) 1142
SchI GAGTC 2 cut(s) 155, 203
ScrFI CCNGG 2 cut(s) 260, 670
SduI GDGCHC 4 cut(s) 30, 606, 1059, 1118
SfaNI GCATC 1 cut(s) 1276
SinI GGWCC 1 cut(s) 1142
SmlI CTYRAG 1 cut(s) 1373
SmoI CTYRAG 1 cut(s) 1373
SphI GCATGC 1 cut(s) 1217
SsiI CCGC 3 cut(s) 177, 407, 902
SspI AATATT 1 cut(s) 1246
SspMI CTAG 2 cut(s) 687, 1536
StyD4I CCNGG 2 cut(s) 258, 668
StyI CCWWGG 2 cut(s) 137, 1400
TaaI ACNGT 2 cut(s) 106, 1467
TaiI ACGT 4 cut(s) 501, 709, 875, 1200
TaqI TCGA 6 cut(s) 11, 335, 479, 1344, 1449, 1569
TatI WGTACW 3 cut(s) 587, 777, 1467
TfiI GAWTC 7 cut(s) 8, 13, 573, 690, 814, 1408, 1668
Tru1I TTAA 9 cut(s) 161, 420, 435, 471, 564, 630, 1062, 1383, 1544
Tru9I TTAA 9 cut(s) 161, 420, 435, 471, 564, 630, 1062, 1383, 1544
TseFI GTSAC 3 cut(s) 494, 1084, 1461
TseI GCWGC 3 cut(s) 1096, 1187, 1396
Tsp45I GTSAC 3 cut(s) 494, 1084, 1461
TspDTI ATGAA 8 cut(s) 17, 504, 726, 827, 981, 1256, 1283, 1466
TspGWI ACGGA 4 cut(s) 38, 299, 1566, 1680
Tth111I GACNNNGTC 2 cut(s) 212, 340
Van91I CCANNNNNTGG 1 cut(s) 615
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 1142
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 3 cut(s) 509, 754, 1007
XceI RCATGY 3 cut(s) 1217, 1369, 1680
XcmI CCANNNNNNNNNTGG 1 cut(s) 1158
XmnI GAANNNNTTC 2 cut(s) 170, 818
XspI CTAG 2 cut(s) 687, 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.