Rw5G050040

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015924
Physical Location & Seq
Reverse (-)
86599971 .. 86601883
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050040.1

Sequence Viewer

Length: 1662 bp
ATGAAAGAATCAAGTCCCAGCAGTGAGCCCATTGGGCAGAACATCATAAAGCTGATAAGCAATCTCTGCTTCTCAGCGTTCGTCTTCTCAGTCCTTATCATCACCGTGATTGCCATCACCTACCAACCCCCAGATCCATGGCTCGAGTCTGCTCCGGCCTTGACAAAACTCTTCACTGAATCCGAGAACGCTACTTTCAAGAATGACAATTCCATCCTTAAGACCGGTGAGGATTTCATTGCCCCGGCTGCCCCTCCGGAGAGCCGGATCACCGAGGCCGTCATTCAGACCACCGAGGCCAACCTCACCAGTTCCCCACAGCCCGAATCGGATTCGGGCTGTGGGGATCTGGAGACAGTGAATTGTTCAGACCCTAGAGTTCTCATTGCGGTTGAGAGGTTTAATTTGAGGCTGTTTAAGACGATTGTGTTTTTGGAGTATCAGAGTCCGGTTAATGGGTCGAAAGCTGATGAGTGTGATGTGCTGTGGAGGTTTAGGAACAAGAAGGAGAAGTCTTGGAGGAGGTATAGGGATTTTAGGAGGTTTAAGTTTGGGTTTGGAGTGAATTGTACCTACAAGGTGGTGCACGCCGGAGGGTGGCATTCCGGTTTGAATGCACGGCGTTCACAGAGGAGCAGGGTTGGTAAGGGGGGTGGTGGAAATGGTAGCAGGATTGTCCCACCGGCTCGTGATGAGGAGATTAATGATACCATACCGAGCTTGGGGGAGAGGAATTTCAGGAGGGGGAAGTACTTGTACTACTCGCGGGGAGGGGATTATTGCAAGGGAATGAATCAGTACATGTGGAGTTTCTTGTGTGGTTTAGGTGAGGCAATGTATTTGAACAGGACATTTGTGGTGGATTTGAGTTTGTGCTTGTCGGGGAGCTATAATCCGAGTAATAAGGATGAGGAAGGGAAGGATTTTCGGTATTATTTTGATTTCGAGCATTTGAAGGAGGTTGCATCAATTGTGGAGGAGGGTGAGTTTTTAAGGGATTGGAAGAAGTGGGATCGGAGCCATAAGAGGAAGCTGCCTGTTAAGAAGGTTGTGAGCCACAAGATTACACCAATGCAACTCAAGAAAGATAGGAACACGGTTCTATGGAGGCAGTTTGATGCGCCGGAGCCAGAGAATTACTGGTACAGAGTGTGCGAAGGACAAGCTGCCAAGTACATTCAGAGGCCGTGGCATGCTTTGTGGAAGTCAAAGAGATTGATGAACATTGTTTCAGAGATTAGTGGGCGAATGGATTGGGATTTCGATGCTGTTCATGTGGTGCGAGGGGAGAAGGCAAAGAATACGCAGCTCTGGCCTCATCTGGAGTATGACACATCCCCCGACCAGCTACTTGAGAAGGTTAAGGGTATGGTTCAGCCTTGGAGGAATCTGTATATAGCCACCAACGAACCTTTTTATAATTACTTTGACAAACTGAGGTCTCAGTACAAGGTTCATTTGCTTGATGATTACAAGGAGTTGTGGAGTAATACAAGCGAGTGGTACAATGAGACTACGACTCTGAACAATGGGAATCCGGTTGAGTTTGATGGGTACATGAGGGTAGAAGTGGATACCGAGGTTCTTTACAGGGCAAAGACACGAGTGGAAACGTTCTATAATTTGACTAAGGATTGCAAGGATGGAGTCTTTACATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

553

Amino Acids

64.24

Weight (kDa)

8.43

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7074 PF23269 118 - 202 2.7e-36 Domain of unknown function (DUF7074)
DUF7075 PF23272 245 - 538 1.2e-62 Domain of unknown function (DUF7075)
O-FucT PF10250 251 - 486 3.8e-06 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08810
fragaria_vesca FvH4_3g45390
malus_domestica MD03G1005400.v1.1 MD11G1008000.v1.1
prunus_persica Prupe.6G005200_v2.0.a1
pyrus_communis pycom03g00560
rosa_chinensis RchiOBHm_Chr5g0083201
rosa_laevigata RLG00000037016
rosa_roxburghii Rroxscaffold_1G00001300
rosa_rugosa Rorug05G0483200.1
rosa_samantha Rh5AG532600 Rh5BG559800 Rh5CG582500 Rh5DG569000
rosa_wichuraiana Rw5G050040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1419
AccII CGCG 1 cut(s) 766
AccIII TCCGGA 1 cut(s) 256
AciI CCGC 2 cut(s) 389, 766
AclI AACGTT 1 cut(s) 1613
AclWI GGATC 4 cut(s) 128, 275, 354, 1020
AcsI RAATTY 1 cut(s) 733
AfaI GTAC 9 cut(s) 571, 752, 758, 800, 1145, 1175, 1448, 1505, 1556
AfiI CCNNNNNNNGG 3 cut(s) 455, 597, 722
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 801
AgeI ACCGGT 1 cut(s) 224
AgsI TTSAA 4 cut(s) 199, 613, 844, 955
AluBI AGCT 8 cut(s) 52, 467, 720, 888, 1033, 1166, 1309, 1348
AluI AGCT 8 cut(s) 52, 467, 720, 888, 1033, 1166, 1309, 1348
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 3 cut(s) 347, 1446, 1505
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 4 cut(s) 128, 275, 354, 1020
Ama87I CYCGRG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 256
AoxI GGCC 5 cut(s) 156, 276, 297, 1184, 1313
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 4 cut(s) 248, 1033, 1166, 1306
ApoI RAATTY 1 cut(s) 733
AseI ATTAAT 1 cut(s) 702
AsiGI ACCGGT 1 cut(s) 224
AspLEI GCGC 1 cut(s) 1123
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 7 cut(s) 94, 109, 239, 262, 298, 839, 995
AvaI CYCGRG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 588
BaeI ACNNNNGTAYC 2 cut(s) 1566, 1599
BanII GRGCYC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BauI CACGAG 2 cut(s) 687, 1602
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 235, 1020, 1153, 1318
BccI CCATC 4 cut(s) 122, 221, 1544, 1637
BceAI ACGGC 3 cut(s) 263, 635, 1171
BciVI GTATCC 1 cut(s) 1567
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 347, 1446, 1505
BfaI CTAG 1 cut(s) 375
BfrI CTTAAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 1567
BglI GCCNNNNNGGC 1 cut(s) 34
BisI GCNGC 4 cut(s) 249, 1034, 1167, 1307
BlsI GCNGC 4 cut(s) 250, 1035, 1168, 1308
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 1019, 1128
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 974, 1108, 1255
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 2 cut(s) 371, 1343
BpuEI CTTGAG 2 cut(s) 1064, 1373
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 1446
BsaJI CCNNGG 7 cut(s) 137, 243, 273, 294, 1187, 1379, 1578
BsaWI WCCGGW 5 cut(s) 224, 256, 448, 605, 1537
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 455, 597, 722
Bse118I RCCGGY 2 cut(s) 224, 682
Bse1I ACTGG 2 cut(s) 309, 1145
Bse3DI GCAATG 3 cut(s) 237, 384, 840
Bse8I GATNNNNATC 1 cut(s) 113
BseAI TCCGGA 1 cut(s) 256
BseDI CCNNGG 7 cut(s) 137, 243, 273, 294, 1187, 1379, 1578
BseGI GGATG 4 cut(s) 213, 913, 1334, 1648
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 3 cut(s) 455, 597, 722
BseMI GCAATG 3 cut(s) 237, 384, 840
BseMII CTCAG 4 cut(s) 87, 102, 1427, 1457
BseNI ACTGG 2 cut(s) 309, 1145
BseRI GAGGAG 4 cut(s) 535, 646, 710, 992
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 4 cut(s) 235, 1020, 1153, 1318
BseYI CCCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 5 cut(s) 158, 278, 299, 1186, 1315
BshTI ACCGGT 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 143
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 3 cut(s) 455, 597, 722
BsmAI GTCTC 3 cut(s) 347, 1446, 1505
BsmFI GGGAC 1 cut(s) 662
BsmI GAATGC 2 cut(s) 601, 619
BsnI GGCC 5 cut(s) 158, 278, 299, 1186, 1315
Bso31I GGTCTC 1 cut(s) 1446
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 30, 588
Bsp13I TCCGGA 1 cut(s) 256
Bsp143I GATC 4 cut(s) 133, 267, 346, 1012
Bsp19I CCATGG 1 cut(s) 137
BspACI CCGC 2 cut(s) 389, 766
BspANI GGCC 5 cut(s) 158, 278, 299, 1186, 1315
BspCNI CTCAG 4 cut(s) 86, 101, 1428, 1456
BspEI TCCGGA 1 cut(s) 256
BspFNI CGCG 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 1019, 1128
BspPI GGATC 4 cut(s) 128, 275, 354, 1020
BspTI CTTAAG 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 1446
BsrDI GCAATG 3 cut(s) 237, 384, 840
BsrFI RCCGGY 2 cut(s) 224, 682
BsrI ACTGG 2 cut(s) 309, 1145
BssAI RCCGGY 2 cut(s) 224, 682
BssECI CCNNGG 7 cut(s) 137, 243, 273, 294, 1187, 1379, 1578
BssMI GATC 4 cut(s) 133, 267, 346, 1012
BssSI CACGAG 2 cut(s) 687, 1602
BssT1I CCWWGG 2 cut(s) 137, 1379
Bst2BI CACGAG 2 cut(s) 687, 1602
Bst4CI ACNGT 3 cut(s) 106, 358, 1099
Bst6I CTCTTC 1 cut(s) 176
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 588, 1194
BstDEI CTNAG 5 cut(s) 73, 88, 1436, 1443, 1629
BstDSI CCRYGG 2 cut(s) 137, 1187
BstF5I GGATG 4 cut(s) 213, 913, 1334, 1648
BstFNI CGCG 1 cut(s) 766
BstHHI GCGC 1 cut(s) 1123
BstKTI GATC 4 cut(s) 136, 270, 349, 1015
BstMAI GTCTC 3 cut(s) 347, 1446, 1505
BstMBI GATC 4 cut(s) 133, 267, 346, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 34, 66, 248, 1312
BstNSI RCATGY 3 cut(s) 805, 1196, 1659
BstSCI CCNGG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 588
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 4 cut(s) 235, 1020, 1153, 1318
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 2 cut(s) 133, 346
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 133, 346
BsuI GTATCC 1 cut(s) 1567
BsuRI GGCC 5 cut(s) 158, 278, 299, 1186, 1315
BtgI CCRYGG 2 cut(s) 137, 1187
BtsCI GGATG 4 cut(s) 213, 913, 1334, 1648
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 28, 174, 363
Cac8I GCNNGC 2 cut(s) 588, 1194
CfoI GCGC 1 cut(s) 1123
Cfr10I RCCGGY 2 cut(s) 224, 682
Csp6I GTAC 9 cut(s) 570, 751, 757, 799, 1144, 1174, 1447, 1504, 1555
CspAI ACCGGT 1 cut(s) 224
CviAII CATG 6 cut(s) 138, 802, 1193, 1274, 1558, 1656
CviQI GTAC 9 cut(s) 570, 751, 757, 799, 1144, 1174, 1447, 1504, 1555
DdeI CTNAG 5 cut(s) 73, 88, 1436, 1443, 1629
DpnI GATC 4 cut(s) 135, 269, 348, 1014
DpnII GATC 4 cut(s) 133, 267, 346, 1012
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 137, 1379
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 1446
Eco88I CYCGRG 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 137, 1379
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 137, 1379
FaeI CATG 6 cut(s) 141, 805, 1196, 1277, 1561, 1659
FaqI GGGAC 1 cut(s) 662
FatI CATG 6 cut(s) 137, 801, 1192, 1273, 1557, 1655
FauI CCCGC 1 cut(s) 759
Fnu4HI GCNGC 4 cut(s) 249, 1034, 1167, 1307
FokI GGATG 4 cut(s) 200, 920, 1321, 1655
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 4 cut(s) 249, 1034, 1167, 1307
FspBI CTAG 1 cut(s) 375
GlaI GCGC 1 cut(s) 1122
GluI GCNGC 4 cut(s) 249, 1034, 1167, 1307
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 2 cut(s) 371, 1343
HaeIII GGCC 5 cut(s) 158, 278, 299, 1186, 1315
HhaI GCGC 1 cut(s) 1123
Hin1II CATG 6 cut(s) 141, 805, 1196, 1277, 1561, 1659
Hin6I GCGC 1 cut(s) 1121
HinP1I GCGC 1 cut(s) 1121
HphI GGTGA 7 cut(s) 94, 109, 239, 262, 298, 839, 995
Hpy166II GTNNAC 2 cut(s) 586, 626
Hpy188III TCNNGA 7 cut(s) 199, 257, 350, 689, 739, 1081, 1322
Hpy8I GTNNAC 2 cut(s) 586, 626
HpyAV CCTTC 8 cut(s) 499, 908, 913, 949, 1039, 1151, 1285, 1351
HpyCH4III ACNGT 3 cut(s) 106, 358, 1099
HpyCH4IV ACGT 1 cut(s) 1613
HpyCH4V TGCA 6 cut(s) 586, 617, 783, 965, 1075, 1638
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 66, 248, 1312
HpyF3I CTNAG 5 cut(s) 73, 88, 1436, 1443, 1629
HpySE526I ACGT 1 cut(s) 1613
Hsp92II CATG 6 cut(s) 141, 805, 1196, 1277, 1561, 1659
HspAI GCGC 1 cut(s) 1121
Kpn2I TCCGGA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 133, 267, 346, 1012
LmnI GCTCC 5 cut(s) 157, 633, 885, 1017, 1126
Lsp1109I GCAGC 4 cut(s) 235, 1020, 1153, 1318
LweI GCATC 3 cut(s) 974, 1108, 1255
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 1613
MalI GATC 4 cut(s) 135, 269, 348, 1014
MboI GATC 4 cut(s) 133, 267, 346, 1012
MboII GAAGA 3 cut(s) 76, 163, 1015
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 2 cut(s) 133, 346
MhlI GDGCHC 2 cut(s) 30, 588
MluCI AATT 9 cut(s) 208, 361, 403, 565, 733, 969, 1135, 1420, 1621
MlyI GAGTC 4 cut(s) 155, 454, 1513, 1656
MroI TCCGGA 1 cut(s) 256
MseI TTAA 9 cut(s) 219, 402, 417, 453, 546, 702, 992, 1041, 1362
MslI CAYNNNNRTG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 969
Mva1269I GAATGC 2 cut(s) 601, 619
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 4 cut(s) 34, 66, 248, 1312
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 1 cut(s) 137
NdeII GATC 4 cut(s) 133, 267, 346, 1012
NlaIII CATG 6 cut(s) 141, 805, 1196, 1277, 1561, 1659
NlaIV GGNNCC 2 cut(s) 1019, 1128
NspI RCATGY 3 cut(s) 805, 1196, 1659
PaeI GCATGC 1 cut(s) 1196
PaeR7I CTCGAG 1 cut(s) 143
PciI ACATGT 1 cut(s) 801
PctI GAATGC 2 cut(s) 601, 619
PfeI GAWTC 7 cut(s) 8, 179, 326, 332, 793, 1387, 1534
PinAI ACCGGT 1 cut(s) 224
PkrI GCNGC 4 cut(s) 250, 1035, 1168, 1308
PleI GAGTC 4 cut(s) 154, 453, 1513, 1655
PpsI GAGTC 4 cut(s) 154, 453, 1513, 1655
PscI ACATGT 1 cut(s) 801
PshBI ATTAAT 1 cut(s) 702
PsiI TTATAA 1 cut(s) 1419
Psp1406I AACGTT 1 cut(s) 1613
PspFI CCCAGC 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 1019, 1128
PspXI VCTCGAGB 1 cut(s) 143
PsuI RGATCY 2 cut(s) 133, 346
RsaI GTAC 9 cut(s) 571, 752, 758, 800, 1145, 1175, 1448, 1505, 1556
RsaNI GTAC 9 cut(s) 570, 751, 757, 799, 1144, 1174, 1447, 1504, 1555
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 9 cut(s) 219, 402, 417, 453, 546, 702, 992, 1041, 1362
SatI GCNGC 4 cut(s) 249, 1034, 1167, 1307
Sau3AI GATC 4 cut(s) 133, 267, 346, 1012
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 4 cut(s) 155, 454, 1513, 1656
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 2 cut(s) 30, 588
SfaNI GCATC 3 cut(s) 974, 1108, 1255
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 4 cut(s) 143, 218, 1079, 1352
SmoI CTYRAG 4 cut(s) 143, 218, 1079, 1352
SphI GCATGC 1 cut(s) 1196
Sse9I AATT 9 cut(s) 208, 361, 403, 565, 733, 969, 1135, 1420, 1621
SsiI CCGC 2 cut(s) 389, 766
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 2 cut(s) 137, 1379
TaaI ACNGT 3 cut(s) 106, 358, 1099
TaiI ACGT 1 cut(s) 1616
TaqI TCGA 4 cut(s) 144, 461, 945, 1263
TasI AATT 9 cut(s) 208, 361, 403, 565, 733, 969, 1135, 1420, 1621
TatI WGTACW 5 cut(s) 750, 756, 798, 1173, 1446
TfiI GAWTC 7 cut(s) 8, 179, 326, 332, 793, 1387, 1534
Tru1I TTAA 9 cut(s) 219, 402, 417, 453, 546, 702, 992, 1041, 1362
Tru9I TTAA 9 cut(s) 219, 402, 417, 453, 546, 702, 992, 1041, 1362
TscAI CASTG 3 cut(s) 28, 181, 363
TseI GCWGC 4 cut(s) 248, 1033, 1166, 1306
TspDTI ATGAA 6 cut(s) 17, 226, 806, 1235, 1262, 1445
TspRI CASTG 3 cut(s) 28, 181, 363
Vha464I CTTAAG 1 cut(s) 218
VneI GTGCAC 1 cut(s) 584
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 1 cut(s) 733
XceI RCATGY 3 cut(s) 805, 1196, 1659
XcmI CCANNNNNNNNNTGG 2 cut(s) 718, 1137
XhoI CTCGAG 1 cut(s) 143
XspI CTAG 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.