Rroxscaffold_1G00001300

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015924
Physical Location & Seq
Reverse (-)
1713474 .. 1718290
4817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00001300.1

Sequence Viewer

Length: 966 bp
ATGTATTTGAACAGGACATTTGTGGTGGATTTGAGTTTGTGCTTGTCGGGGAGTTATAATCCGAGTAATAAGGATGAGGAAGGGAAGGATTTTCGGTATTATTTTGATTTCGAGCATTTGAAGGAGGTTGCATCAATTGTGGAGGAGGGTGAGTTTTTAAGGGATTGGAAGAAGTGGGATCGGAGCCATAAGAGGAAGCTGCCTGTTAAGAAGGTTTTGAGCCACAAGATTACGCCAATGCAACTCAAGAAAGATAGGAACACTGTTCTATGGAGGCAGTTCGATGCGCCGGAGCCAGAGAATTACTGGTACAGGGTGTGCGAAGGACAAGCTGCCAAGTACATTCAGAGGCCATGGCATGCTTTGTGGAAGTCAAAGAGATTGATGAACATTGTTTCAGAGATCAGTGGGCGAATGGATTGGGATTTCGATGCTGTTCATGTGGTGCGAGGGGAGAAGGCGAAGAATACGCAGCTCTGGCCTCATCTGGATTATGATACATCCCCCGACCAGCTACTTGAGAAGGTTAAGGGTATGGTTCAGCCTTGGAGGAATCTGTATATAGCCACCAATGAACCTTTTTATAATTACTTTGACAAACTGAGGTCTCAGTACAAGGTTCATTTGCTTGATGATTACAAGGAGTTGTGGAGTAATACAAGCGAGTGGTACAATGAGACTACGACTCTGAACAATGGGAATCCGGTTGAGTTTGATGGGTACATGAGGGTAGAAGTGGATACTGAGGTTCTTTACAGGGCAAAGACGCGAGTGGAAACGTTCTATAATTTGACAAAGGATTGCAAGGATGGAACGGGGTTGGTGAAGAATGTGATTTCTTGGACGTGGTGCAGGCTGCGGGGTTTGGTGGAAAAACGCCTGCTCTCGGTTGAAAGATTTGAATTTGGGGAAAGAGATTCATGGGTATGTGGTGAAACATGGAATGGGAGGGAATGTGTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

321

Amino Acids

38.53

Weight (kDa)

7.57

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7075 PF23272 2 - 260 1.1e-49 Domain of unknown function (DUF7075)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08810
fragaria_vesca FvH4_3g45390
malus_domestica MD03G1005400.v1.1 MD11G1008000.v1.1
prunus_persica Prupe.6G005200_v2.0.a1
pyrus_communis pycom03g00560
rosa_chinensis RchiOBHm_Chr5g0083201
rosa_laevigata RLG00000037016
rosa_roxburghii Rroxscaffold_1G00001300
rosa_rugosa Rorug05G0483200.1
rosa_samantha Rh5AG532600 Rh5BG559800 Rh5CG582500 Rh5DG569000
rosa_wichuraiana Rw5G050040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 57, 585
AccII CGCG 1 cut(s) 769
AciI CCGC 1 cut(s) 859
AclI AACGTT 1 cut(s) 779
AclWI GGATC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 902
AfaI GTAC 5 cut(s) 311, 341, 614, 671, 722
AfiI CCNNNNNNNGG 1 cut(s) 886
AgsI TTSAA 4 cut(s) 10, 121, 893, 902
AjiI CACGTC 1 cut(s) 846
AluBI AGCT 4 cut(s) 199, 332, 475, 514
AluI AGCT 4 cut(s) 199, 332, 475, 514
Alw26I GTCTC 2 cut(s) 612, 671
AlwI GGATC 1 cut(s) 186
AoxI GGCC 2 cut(s) 350, 479
ApeKI GCWGC 4 cut(s) 199, 332, 472, 856
ApoI RAATTY 1 cut(s) 902
AspLEI GCGC 1 cut(s) 289
AsuHPI GGTGA 3 cut(s) 161, 835, 944
BaeI ACNNNNGTAYC 2 cut(s) 732, 765
BbvI GCAGC 4 cut(s) 186, 319, 484, 843
BccI CCATC 2 cut(s) 710, 803
BciVI GTATCC 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 612, 671
BfuI GTATCC 1 cut(s) 733
BisI GCNGC 4 cut(s) 200, 333, 473, 857
BlsI GCNGC 4 cut(s) 201, 334, 474, 858
BmgBI CACGTC 1 cut(s) 846
BmiI GGNNCC 2 cut(s) 185, 294
BmsI GCATC 3 cut(s) 140, 274, 421
BpuEI CTTGAG 2 cut(s) 230, 539
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 2 cut(s) 353, 545
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 3 cut(s) 137, 167, 940
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse1I ACTGG 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 353, 545
BseGI GGATG 3 cut(s) 79, 500, 814
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMII CTCAG 3 cut(s) 593, 623, 735
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 4 cut(s) 186, 319, 484, 843
BsgI GTGCAG 1 cut(s) 871
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 2 cut(s) 352, 481
BsiSI CCGG 2 cut(s) 290, 704
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 2 cut(s) 612, 671
BsnI GGCC 2 cut(s) 352, 481
Bso31I GGTCTC 1 cut(s) 612
Bsp143I GATC 2 cut(s) 178, 402
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 1 cut(s) 859
BspANI GGCC 2 cut(s) 352, 481
BspCNI CTCAG 3 cut(s) 594, 622, 736
BspFNI CGCG 1 cut(s) 769
BspLI GGNNCC 2 cut(s) 185, 294
BspPI GGATC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 612
BsrI ACTGG 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 353, 545
BssMI GATC 2 cut(s) 178, 402
BssT1I CCWWGG 2 cut(s) 353, 545
Bst4CI ACNGT 1 cut(s) 265
BstC8I GCNNGC 3 cut(s) 360, 854, 881
BstDEI CTNAG 3 cut(s) 602, 609, 744
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 3 cut(s) 79, 500, 814
BstFNI CGCG 1 cut(s) 769
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 181, 405
BstMAI GTCTC 2 cut(s) 612, 671
BstMBI GATC 2 cut(s) 178, 402
BstMWI GCNNNNNNNGC 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 362
BstUI CGCG 1 cut(s) 769
BstV1I GCAGC 4 cut(s) 186, 319, 484, 843
BsuI GTATCC 1 cut(s) 733
BsuRI GGCC 2 cut(s) 352, 481
BtgI CCRYGG 1 cut(s) 353
BtrI CACGTC 1 cut(s) 846
BtsCI GGATG 3 cut(s) 79, 500, 814
BtsIMutI CAGTG 2 cut(s) 261, 412
Cac8I GCNNGC 3 cut(s) 360, 854, 881
CfoI GCGC 1 cut(s) 289
CseI GACGC 1 cut(s) 775
Csp6I GTAC 5 cut(s) 310, 340, 613, 670, 721
CviAII CATG 6 cut(s) 354, 359, 440, 724, 921, 939
CviQI GTAC 5 cut(s) 310, 340, 613, 670, 721
DdeI CTNAG 3 cut(s) 602, 609, 744
DpnI GATC 2 cut(s) 180, 404
DpnII GATC 2 cut(s) 178, 402
Eco130I CCWWGG 2 cut(s) 353, 545
Eco31I GGTCTC 1 cut(s) 612
EcoT14I CCWWGG 2 cut(s) 353, 545
ErhI CCWWGG 2 cut(s) 353, 545
FaeI CATG 6 cut(s) 357, 362, 443, 727, 924, 942
FatI CATG 6 cut(s) 353, 358, 439, 723, 920, 938
FauI CCCGC 1 cut(s) 852
Fnu4HI GCNGC 4 cut(s) 200, 333, 473, 857
FokI GGATG 3 cut(s) 86, 487, 821
Fsp4HI GCNGC 4 cut(s) 200, 333, 473, 857
GlaI GCGC 1 cut(s) 288
GluI GCNGC 4 cut(s) 200, 333, 473, 857
HaeIII GGCC 2 cut(s) 352, 481
HapII CCGG 2 cut(s) 290, 704
HgaI GACGC 1 cut(s) 775
HhaI GCGC 1 cut(s) 289
Hin1II CATG 6 cut(s) 357, 362, 443, 727, 924, 942
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HinfI GANTC 4 cut(s) 553, 685, 700, 917
HpaII CCGG 2 cut(s) 290, 704
HphI GGTGA 3 cut(s) 161, 835, 944
Hpy188I TCNGA 5 cut(s) 63, 183, 348, 400, 690
Hpy188III TCNNGA 2 cut(s) 247, 488
HpyAV CCTTC 7 cut(s) 74, 79, 115, 205, 317, 451, 517
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 2 cut(s) 779, 845
HpyCH4V TGCA 4 cut(s) 131, 241, 804, 852
HpyF10VI GCNNNNNNNGC 1 cut(s) 478
HpyF3I CTNAG 3 cut(s) 602, 609, 744
HpySE526I ACGT 2 cut(s) 779, 845
Hsp92II CATG 6 cut(s) 357, 362, 443, 727, 924, 942
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 178, 402
LmnI GCTCC 2 cut(s) 183, 292
Lsp1109I GCAGC 4 cut(s) 186, 319, 484, 843
LweI GCATC 3 cut(s) 140, 274, 421
MaeII ACGT 2 cut(s) 779, 845
MalI GATC 2 cut(s) 180, 404
MboI GATC 2 cut(s) 178, 402
MboII GAAGA 3 cut(s) 181, 475, 838
MfeI CAATTG 1 cut(s) 135
MluCI AATT 5 cut(s) 135, 301, 586, 787, 902
MlyI GAGTC 1 cut(s) 679
MseI TTAA 3 cut(s) 158, 207, 528
MslI CAYNNNNRTG 1 cut(s) 925
MspI CCGG 2 cut(s) 290, 704
MunI CAATTG 1 cut(s) 135
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 1 cut(s) 478
NcoI CCATGG 1 cut(s) 353
NdeII GATC 2 cut(s) 178, 402
NlaIII CATG 6 cut(s) 357, 362, 443, 727, 924, 942
NlaIV GGNNCC 2 cut(s) 185, 294
NspI RCATGY 1 cut(s) 362
PaeI GCATGC 1 cut(s) 362
PfeI GAWTC 3 cut(s) 553, 700, 917
PkrI GCNGC 4 cut(s) 201, 334, 474, 858
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
PsiI TTATAA 2 cut(s) 57, 585
Psp1406I AACGTT 1 cut(s) 779
PspN4I GGNNCC 2 cut(s) 185, 294
RsaI GTAC 5 cut(s) 311, 341, 614, 671, 722
RsaNI GTAC 5 cut(s) 310, 340, 613, 670, 721
RseI CAYNNNNRTG 1 cut(s) 925
SaqAI TTAA 3 cut(s) 158, 207, 528
SatI GCNGC 4 cut(s) 200, 333, 473, 857
Sau3AI GATC 2 cut(s) 178, 402
SchI GAGTC 1 cut(s) 679
SfaNI GCATC 3 cut(s) 140, 274, 421
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 2 cut(s) 245, 518
SmoI CTYRAG 2 cut(s) 245, 518
SphI GCATGC 1 cut(s) 362
Sse9I AATT 5 cut(s) 135, 301, 586, 787, 902
SsiI CCGC 1 cut(s) 859
StyI CCWWGG 2 cut(s) 353, 545
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 2 cut(s) 782, 848
TaqI TCGA 3 cut(s) 111, 282, 429
TasI AATT 5 cut(s) 135, 301, 586, 787, 902
TatI WGTACW 2 cut(s) 339, 612
TfiI GAWTC 3 cut(s) 553, 700, 917
Tru1I TTAA 3 cut(s) 158, 207, 528
Tru9I TTAA 3 cut(s) 158, 207, 528
TscAI CASTG 2 cut(s) 268, 412
TseI GCWGC 4 cut(s) 199, 332, 472, 856
TspDTI ATGAA 5 cut(s) 401, 428, 588, 611, 909
TspRI CASTG 2 cut(s) 268, 412
XapI RAATTY 1 cut(s) 902
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.