Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0022323

Physical Location & Seq

Genomic Coordinates Reverse (-)

3260098 .. 3264559

Gene Structure 4462 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1041000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 561 bp

ATGTGGAGTAAGTCATCAAGTTTTCTTCACACCAATAAAATAGCTGCTGCAAATTTGGTCGGAGGTCTTAGATTCGCCACCGCTGCCGTCAACCGCTACAATGGTTCAACCTCAAGGTCTCTCTTCGGGCATGGCTATTTCTTTCGCTGCCAGGAAAAAATTCCTCCCAATAGTAGGCGGACGCGGGGCCAAACCTACGGACAATGCTTCGGGTTCGACCAACGCGGCCATCCATCTCCTTATTCTATGGATCATCCCATGAATATGGCTCCTGCCCTTTTTGCCGTGGAAGAGTATAACAAGAAAAAGAATGCCAAACTGCATTTTGTGAGAGTTGTCCACGCTAGGCAGATGCTTTTTCATGGTGATTTCATTCAAAATGTATACTTTGATGCGGTTGATGGGGGTGTGACCGAAATGTACAATGCAAGAGTTCAGAGCAGATTCGGCTGCAATATGAAGCTGATATCATTTGGCGGAGGTAAACACAAGAAGCACGAGTTTGTGGAAAAGACGATTCGCTATGGAACTTCTACTACCAAAATTGCACATTTTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

187

Amino Acids

21.19

Weight (kDa)

10.01

Isoelectric Point (pI)

31.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQAPI	PF16845	92 - 158	5.2e-06	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022323)

Species	Orthologous Gene IDs
malus_domestica	MD03G1041000.v1.1 MD14G1175500.v1.1
pyrus_communis	pycom03g03170 pycom03g03180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	384
AccII	CGCG	2 cut(s)	184, 225
AciI	CCGC	7 cut(s)	81, 94, 178, 184, 225, 395, 477
AclWI	GGATC	1 cut(s)	258
AcoI	YGGCCR	1 cut(s)	226
AcsI	RAATTY	2 cut(s)	52, 159
AfaI	GTAC	1 cut(s)	422
AfiI	CCNNNNNNNGG	1 cut(s)	174
AgsI	TTSAA	2 cut(s)	108, 377
AjnI	CCWGG	1 cut(s)	150
AluBI	AGCT	2 cut(s)	44, 463
AluI	AGCT	2 cut(s)	44, 463
Alw26I	GTCTC	1 cut(s)	123
AlwI	GGATC	1 cut(s)	258
AoxI	GGCC	2 cut(s)	187, 226
ApeKI	GCWGC	5 cut(s)	44, 47, 83, 147, 450
ApoI	RAATTY	2 cut(s)	52, 159
Asp700I	GAANNNNTTC	1 cut(s)	159
AspS9I	GGNCC	1 cut(s)	187
AsuHPI	GGTGA	1 cut(s)	377
BauI	CACGAG	1 cut(s)	497
BbvI	GCAGC	5 cut(s)	31, 34, 70, 134, 437
BccI	CCATC	3 cut(s)	237, 241, 395
BceAI	ACGGC	2 cut(s)	71, 269
BciT130I	CCWGG	1 cut(s)	152
BcoDI	GTCTC	1 cut(s)	123
BfaI	CTAG	1 cut(s)	345
BisI	GCNGC	6 cut(s)	45, 48, 84, 148, 226, 451
BlsI	GCNGC	6 cut(s)	46, 49, 85, 149, 227, 452
Bme1390I	CCNGG	1 cut(s)	152
BmgT120I	GGNCC	1 cut(s)	187
BmiI	GGNNCC	2 cut(s)	188, 270
BmrFI	CCNGG	1 cut(s)	152
BmsI	GCATC	2 cut(s)	342, 382
BpuEI	CTTGAG	1 cut(s)	97
BsaI	GGTCTC	1 cut(s)	123
BsaJI	CCNNGG	1 cut(s)	285
Bsc4I	CCNNNNNNNGG	1 cut(s)	174
BseBI	CCWGG	1 cut(s)	152
BseDI	CCNNGG	1 cut(s)	285
BseGI	GGATG	2 cut(s)	229, 253
BseLI	CCNNNNNNNGG	1 cut(s)	174
BseXI	GCAGC	5 cut(s)	31, 34, 70, 134, 437
Bsh1236I	CGCG	2 cut(s)	184, 225
BshFI	GGCC	2 cut(s)	189, 228
BslI	CCNNNNNNNGG	1 cut(s)	174
BsmAI	GTCTC	1 cut(s)	123
BsmI	GAATGC	1 cut(s)	316
BsnI	GGCC	2 cut(s)	189, 228
Bso31I	GGTCTC	1 cut(s)	123
Bsp1407I	TGTACA	1 cut(s)	420
Bsp143I	GATC	1 cut(s)	250
BspACI	CCGC	7 cut(s)	81, 94, 178, 184, 225, 395, 477
BspANI	GGCC	2 cut(s)	189, 228
BspFNI	CGCG	2 cut(s)	184, 225
BspLI	GGNNCC	2 cut(s)	188, 270
BspPI	GGATC	1 cut(s)	258
BspTNI	GGTCTC	1 cut(s)	123
BsrGI	TGTACA	1 cut(s)	420
BssECI	CCNNGG	1 cut(s)	285
BssMI	GATC	1 cut(s)	250
BssNAI	GTATAC	1 cut(s)	385
BssSI	CACGAG	1 cut(s)	497
Bst1107I	GTATAC	1 cut(s)	385
Bst2BI	CACGAG	1 cut(s)	497
Bst2UI	CCWGG	1 cut(s)	152
Bst6I	CTCTTC	2 cut(s)	128, 285
BstAUI	TGTACA	1 cut(s)	420
BstDEI	CTNAG	1 cut(s)	68
BstDSI	CCRYGG	1 cut(s)	285
BstF5I	GGATG	2 cut(s)	229, 253
BstFNI	CGCG	2 cut(s)	184, 225
BstKTI	GATC	1 cut(s)	253
BstMAI	GTCTC	1 cut(s)	123
BstMBI	GATC	1 cut(s)	250
BstMWI	GCNNNNNNNGC	3 cut(s)	83, 281, 447
BstNI	CCWGG	1 cut(s)	152
BstSCI	CCNGG	1 cut(s)	150
BstUI	CGCG	2 cut(s)	184, 225
BstV1I	GCAGC	5 cut(s)	31, 34, 70, 134, 437
BstXI	CCANNNNNNTGG	1 cut(s)	265
BstZ17I	GTATAC	1 cut(s)	385
BsuRI	GGCC	2 cut(s)	189, 228
BtgI	CCRYGG	1 cut(s)	285
BtsCI	GGATG	2 cut(s)	229, 253
Cfr13I	GGNCC	1 cut(s)	187
CseI	GACGC	1 cut(s)	190
Csp6I	GTAC	1 cut(s)	421
CviAII	CATG	3 cut(s)	131, 259, 362
CviJI	RGCY	7 cut(s)	44, 135, 189, 228, 269, 450, 463
CviKI_1	RGCY	7 cut(s)	44, 135, 189, 228, 269, 450, 463
CviQI	GTAC	1 cut(s)	421
DdeI	CTNAG	1 cut(s)	68
DpnI	GATC	1 cut(s)	252
DpnII	GATC	1 cut(s)	250
EaeI	YGGCCR	1 cut(s)	226
Eam1104I	CTCTTC	2 cut(s)	128, 285
EarI	CTCTTC	2 cut(s)	128, 285
EciI	GGCGGA	2 cut(s)	193, 492
Eco31I	GGTCTC	1 cut(s)	123
Eco32I	GATATC	1 cut(s)	468
EcoRII	CCWGG	1 cut(s)	150
EcoRV	GATATC	1 cut(s)	468
FaeI	CATG	3 cut(s)	134, 262, 365
FaiI	YATR	9 cut(s)	132, 248, 260, 266, 297, 363, 385, 458, 525
FatI	CATG	3 cut(s)	130, 258, 361
FauI	CCCGC	1 cut(s)	177
FblI	GTMKAC	1 cut(s)	384
Fnu4HI	GCNGC	6 cut(s)	45, 48, 84, 148, 226, 451
FokI	GGATG	2 cut(s)	216, 240
Fsp4HI	GCNGC	6 cut(s)	45, 48, 84, 148, 226, 451
FspBI	CTAG	1 cut(s)	345
GluI	GCNGC	6 cut(s)	45, 48, 84, 148, 226, 451
HaeIII	GGCC	2 cut(s)	189, 228
HgaI	GACGC	1 cut(s)	190
Hin1II	CATG	3 cut(s)	134, 262, 365
HincII	GTYRAC	1 cut(s)	91
HindII	GTYRAC	1 cut(s)	91
HinfI	GANTC	3 cut(s)	72, 444, 517
HphI	GGTGA	1 cut(s)	377
Hpy166II	GTNNAC	4 cut(s)	91, 340, 385, 485
Hpy188I	TCNGA	2 cut(s)	62, 438
Hpy8I	GTNNAC	4 cut(s)	91, 340, 385, 485
HpyCH4V	TGCA	5 cut(s)	50, 322, 428, 453, 548
HpyF10VI	GCNNNNNNNGC	3 cut(s)	83, 281, 447
HpyF3I	CTNAG	1 cut(s)	68
Hsp92II	CATG	3 cut(s)	134, 262, 365
Kzo9I	GATC	1 cut(s)	250
LmnI	GCTCC	1 cut(s)	274
LpnPI	CCDG	3 cut(s)	137, 164, 285
Lsp1109I	GCAGC	5 cut(s)	31, 34, 70, 134, 437
LweI	GCATC	2 cut(s)	342, 382
MaeI	CTAG	1 cut(s)	345
MaeIII	GTNAC	1 cut(s)	409
MalI	GATC	1 cut(s)	252
MboI	GATC	1 cut(s)	250
MboII	GAAGA	3 cut(s)	17, 115, 302
MluCI	AATT	3 cut(s)	52, 159, 543
MmeI	TCCRAC	1 cut(s)	40
MnlI	CCTC	4 cut(s)	56, 121, 174, 473
MroXI	GAANNNNTTC	1 cut(s)	159
MseI	TTAA	1 cut(s)	559
MslI	CAYNNNNRTG	1 cut(s)	263
MspA1I	CMGCKG	1 cut(s)	83
MspR9I	CCNGG	1 cut(s)	152
Mva1269I	GAATGC	1 cut(s)	316
MvaI	CCWGG	1 cut(s)	152
MvnI	CGCG	2 cut(s)	184, 225
MwoI	GCNNNNNNNGC	3 cut(s)	83, 281, 447
NdeII	GATC	1 cut(s)	250
NlaIII	CATG	3 cut(s)	134, 262, 365
NlaIV	GGNNCC	2 cut(s)	188, 270
NmuCI	GTSAC	1 cut(s)	409
PctI	GAATGC	1 cut(s)	316
PdmI	GAANNNNTTC	1 cut(s)	159
PfeI	GAWTC	3 cut(s)	72, 444, 517
PkrI	GCNGC	6 cut(s)	46, 49, 85, 149, 227, 452
Psp6I	CCWGG	1 cut(s)	150
PspGI	CCWGG	1 cut(s)	150
PspN4I	GGNNCC	2 cut(s)	188, 270
PspPI	GGNCC	1 cut(s)	187
PsrI	GAACNNNNNNTAC	2 cut(s)	520, 552
RsaI	GTAC	1 cut(s)	422
RsaNI	GTAC	1 cut(s)	421
RseI	CAYNNNNRTG	1 cut(s)	263
SaqAI	TTAA	1 cut(s)	559
SatI	GCNGC	6 cut(s)	45, 48, 84, 148, 226, 451
Sau3AI	GATC	1 cut(s)	250
Sau96I	GGNCC	1 cut(s)	187
ScrFI	CCNGG	1 cut(s)	152
SetI	ASST	7 cut(s)	46, 67, 113, 119, 197, 465, 484
SfaNI	GCATC	2 cut(s)	342, 382
SmiMI	CAYNNNNRTG	1 cut(s)	263
SmlI	CTYRAG	1 cut(s)	112
SmoI	CTYRAG	1 cut(s)	112
Sse9I	AATT	3 cut(s)	52, 159, 543
SsiI	CCGC	7 cut(s)	81, 94, 178, 184, 225, 395, 477
SspMI	CTAG	1 cut(s)	345
StyD4I	CCNGG	1 cut(s)	150
TaqI	TCGA	1 cut(s)	216
TaqII	GACCGA	1 cut(s)	428
TasI	AATT	3 cut(s)	52, 159, 543
TatI	WGTACW	1 cut(s)	420
TauI	GCSGC	1 cut(s)	228
TfiI	GAWTC	3 cut(s)	72, 444, 517
Tru1I	TTAA	1 cut(s)	559
Tru9I	TTAA	1 cut(s)	559
TseFI	GTSAC	1 cut(s)	409
TseI	GCWGC	5 cut(s)	44, 47, 83, 147, 450
Tsp45I	GTSAC	1 cut(s)	409
TspDTI	ATGAA	4 cut(s)	275, 350, 361, 473
TspGWI	ACGGA	1 cut(s)	213
XapI	RAATTY	2 cut(s)	52, 159
XmiI	GTMKAC	1 cut(s)	384
XmnI	GAANNNNTTC	1 cut(s)	159
XspI	CTAG	1 cut(s)	345

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.