MD03G1041800.v1.1

Phosphatidylinositol phosphatidylcholine transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003880
Physical Location & Seq
Forward (+)
3307535 .. 3312945
5411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1041800.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGTCGATGTCAGGCCTTGAAGGGTTTGGAGCTCTTGAGGAAGTCCGAGAGAGAAGATCCGATTTTGAGATTTCTGAAGAGGAGAGAAGACGCTCAAGAATTTGTAATCTCAAGAAGAAGGCGATAAATGCTTCCAGTAAGTTTACCCATTCTCTTAAAAAAAGAGGAAAAAGGAAAATCGATTACCGGGTTCCATCAGTTTCCATACAAGATGTACGGGATGCGAAAGAGGAGAGTGTTGTGCAAGAGTTTCGTCAAAGACTCCTTGACATGGACTTGTTGCCAGCAAGGCACGATGACTACCATACTTTGTTGAGATTTTTGAAAGCTAGAGACCTTAACATTGAAAAAACACTCCGGTTGTGGGAAGAAATGCTTAAATGGAGAAAAGAATTTGGAACAGACACCATTTTGGAGGATTTTGAATTTGAAGAGCTTGATGAAGTAGTGCAATATTACCCTCAAGGATACCATGGGGTTGATAAAGATGGCAGACCTGTTTACATTGAGAGACTGGGAAAAGCTCATCCAAGTAGGCTAATGCAAATCACCAGAATAGAGCGATACTTAAAGTACCATGTCCAAGAGTTTGAGAGAGCTCTGCAGGAGAAATTCCCTGCTTGTTCAATTGCAGCAAAGAGACAGATATGTTCAACAACTACTATACTGGATGTACAAGGCTTGGGCATGAAAAATTTCACTAGGACTGCTGCAAATCTTTTGTCTGCCATGACAAAGATAGACACCAATTACTACCCTGAGACTCTACAACGAATGTACATTGTCAATGCTGGTCCTGGCTTTAAGAAGATGCTTTGGCCTGCTGCACAGAAGTTTCTTGATGTAAAGACTATTGCAAAGATACAGGTTTTGGATCCCAAATCCTTGTCTAAACTACTGGAAGTCATTGATTCAAGTCAGTTGCCAGACTTCCTGGGCGGATCATGTACATGTTCAGCTGAAGGGGGTTGCCTTAGGTCTAATAAAGGTCCATGGAATGACATCGAGATAATGAAGCTTCTGCATGCTGCCGAAGCAACATTTGTGAGGCAAATTTCTAGTGTGTCTGGTGAACAGGAGAAATTTGATTCATATATTCAAATACACCCACTGAAGGGTAGAACAAGCAATACATCAGTTGTAGAATCAGGGTCAGATATTGATGATCCTTGTTCTTCACTTGGACGAAGGAGTTCTGCATTTCCTCCTTTGGACGCAGTTGATGAAGAAGCATTAGATCCAAATGCATTCTACAGCAGAGATGATCTTCCCTGGGTTGAGAAAGCCAGAACAAGTGATCAAGGAGTTCAATGTCATCAGGATCAGAATGTTTCTTGTGAAGCAACATTAAATTCAGAAGGTAGTTCAGTTAGGTTTGACGCAGCTGAGGATAAAATTGAGAAGACGGACTTCACTCGTGTGGCAAGAAAGCTGATATCTTTCGTGATCAGACTAATACCATTTTCTCATATTCTACAATTTGAATTTTGGAGAAGGCAGAACACCATTTACCCATCGAAGTTGGTGGAAAGCAACACGAATGGTCATTTACCAGCCCCTGAAGTTGTGAGTCAAGAAGACTATGTCCGCCCGTGTTTACAGCGTCTTCAGCAACTAGAAAAAGTATGTGAGGAGCTTAAAAACAAGCCTGCTGCAATTCCTTTAGAAAAGGAGCGAATGCTGATGGACTCGATGGACAGGATTAAGTCTGTTGAATATGATCTCGAGCAAACAAAAGGAGTGCTGCACGCTGCAGTTGTAAAGCAACTAGAGATTGCTAGGTTGTTGGAAAATCTACAGGAATCTAAATTTCATAGAAAGAGACCGTTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

612

Amino Acids

70.3

Weight (kDa)

6.91

Isoelectric Point (pI)

57.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 151 - 314 2.3e-28 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 939, 1588
AclWI GGATC 7 cut(s) 51, 869, 882, 949, 1160, 1232, 1329
AcuI CTGAAG 5 cut(s) 96, 981, 1133, 1581, 1592
AfaI GTAC 5 cut(s) 216, 575, 675, 779, 949
AfiI CCNNNNNNNGG 4 cut(s) 271, 364, 1114, 1115
AflIII ACRYGT 1 cut(s) 950
AjnI CCWGG 3 cut(s) 796, 933, 1271
AleI CACNNNNGTG 1 cut(s) 1418
Alw21I GWGCWC 2 cut(s) 34, 601
Alw26I GTCTC 5 cut(s) 327, 505, 634, 755, 1816
AlwI GGATC 7 cut(s) 51, 869, 882, 949, 1160, 1232, 1329
AlwNI CAGNNNCTG 1 cut(s) 1559
Ama87I CYCGRG 1 cut(s) 1724
AoxI GGCC 2 cut(s) 13, 818
ApeKI GCWGC 8 cut(s) 632, 710, 824, 1028, 1382, 1652, 1744, 1751
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 2 cut(s) 695, 1192
AspS9I GGNCC 2 cut(s) 794, 989
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 541, 1082
AvaI CYCGRG 1 cut(s) 1724
AvaII GGWCC 2 cut(s) 794, 989
AxyI CCTNAGG 1 cut(s) 974
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 2 cut(s) 34, 601
BauI CACGAG 1 cut(s) 1416
BbsI GAAGAC 4 cut(s) 94, 1409, 1584, 1598
Bbv12I GWGCWC 2 cut(s) 34, 601
BbvCI CCTCAGC 1 cut(s) 1386
BbvI GCAGC 8 cut(s) 644, 697, 811, 1015, 1394, 1639, 1731, 1738
BccI CCATC 5 cut(s) 202, 482, 1522, 1678, 1687
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 3 cut(s) 798, 935, 1273
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 2 cut(s) 1297, 1446
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 5 cut(s) 327, 505, 634, 755, 1816
BfaI CTAG 6 cut(s) 330, 702, 1059, 1616, 1769, 1779
BfmI CTRYAG 4 cut(s) 602, 1252, 1752, 1796
BfuI GTATCC 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 289
BisI GCNGC 8 cut(s) 633, 711, 825, 1029, 1383, 1653, 1745, 1752
BlsI GCNGC 8 cut(s) 634, 712, 826, 1030, 1384, 1654, 1746, 1753
Bme1390I CCNGG 4 cut(s) 188, 798, 935, 1273
Bme18I GGWCC 2 cut(s) 794, 989
BmeT110I CYCGRG 1 cut(s) 1724
BmgT120I GGNCC 2 cut(s) 794, 989
BmiI GGNNCC 2 cut(s) 192, 876
BmrFI CCNGG 4 cut(s) 188, 798, 935, 1273
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 2 cut(s) 211, 801
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 4 cut(s) 94, 1409, 1584, 1598
Bpu10I CCTNAGC 1 cut(s) 1386
BpuEI CTTGAG 4 cut(s) 56, 79, 95, 447
BpuMI CCSGG 1 cut(s) 188
Bsa29I ATCGAT 1 cut(s) 180
BsaI GGTCTC 2 cut(s) 327, 1816
BsaJI CCNNGG 5 cut(s) 472, 934, 992, 1271, 1272
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 364, 1114, 1115
Bse1I ACTGG 4 cut(s) 135, 519, 672, 903
Bse21I CCTNAGG 1 cut(s) 974
BseBI CCWGG 3 cut(s) 798, 935, 1273
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 5 cut(s) 472, 934, 992, 1271, 1272
BseGI GGATG 3 cut(s) 226, 526, 676
BseLI CCNNNNNNNGG 4 cut(s) 271, 364, 1114, 1115
BseMII CTCAG 2 cut(s) 750, 1377
BseNI ACTGG 4 cut(s) 135, 519, 672, 903
BseRI GAGGAG 3 cut(s) 95, 245, 1646
BseXI GCAGC 8 cut(s) 644, 697, 811, 1015, 1394, 1639, 1731, 1738
BsgI GTGCAG 2 cut(s) 810, 1730
BshFI GGCC 2 cut(s) 15, 820
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 34, 601
BsiHKCI CYCGRG 1 cut(s) 1724
BsiSI CCGG 2 cut(s) 187, 358
BslI CCNNNNNNNGG 4 cut(s) 271, 364, 1114, 1115
BsmAI GTCTC 5 cut(s) 327, 505, 634, 755, 1816
BsmI GAATGC 2 cut(s) 1247, 1683
BsnI GGCC 2 cut(s) 15, 820
Bso31I GGTCTC 2 cut(s) 327, 1816
BsoBI CYCGRG 1 cut(s) 1724
Bsp1286I GDGCHC 2 cut(s) 34, 601
Bsp1407I TGTACA 3 cut(s) 673, 777, 947
Bsp19I CCATGG 2 cut(s) 472, 992
BspACI CCGC 2 cut(s) 939, 1588
BspANI GGCC 2 cut(s) 15, 820
BspCNI CTCAG 2 cut(s) 751, 1378
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 192, 876
BspMAI CTGCAG 2 cut(s) 606, 1756
BspPI GGATC 7 cut(s) 51, 869, 882, 949, 1160, 1232, 1329
BspQI GCTCTTC 1 cut(s) 426
BspTNI GGTCTC 2 cut(s) 327, 1816
BsrGI TGTACA 3 cut(s) 673, 777, 947
BsrI ACTGG 4 cut(s) 135, 519, 672, 903
BssECI CCNNGG 5 cut(s) 472, 934, 992, 1271, 1272
BssSI CACGAG 1 cut(s) 1416
BssT1I CCWWGG 2 cut(s) 472, 992
Bst2BI CACGAG 1 cut(s) 1416
Bst2UI CCWGG 3 cut(s) 798, 935, 1273
Bst4CI ACNGT 1 cut(s) 1827
Bst6I CTCTTC 2 cut(s) 72, 426
BstAUI TGTACA 3 cut(s) 673, 777, 947
BstC8I GCNNGC 5 cut(s) 285, 822, 1026, 1650, 1749
BstDEI CTNAG 3 cut(s) 759, 974, 1386
BstDSI CCRYGG 2 cut(s) 472, 992
BstF5I GGATG 3 cut(s) 226, 526, 676
BstMAI GTCTC 5 cut(s) 327, 505, 634, 755, 1816
BstMWI GCNNNNNNNGC 4 cut(s) 128, 289, 1034, 1609
BstNI CCWGG 3 cut(s) 798, 935, 1273
BstNSI RCATGY 2 cut(s) 954, 1028
BstSCI CCNGG 4 cut(s) 186, 796, 933, 1271
BstSFI CTRYAG 4 cut(s) 602, 1252, 1752, 1796
BstV1I GCAGC 8 cut(s) 644, 697, 811, 1015, 1394, 1639, 1731, 1738
BstV2I GAAGAC 4 cut(s) 94, 1409, 1584, 1598
BstX2I RGATCY 3 cut(s) 56, 874, 1237
BstYI RGATCY 3 cut(s) 56, 874, 1237
Bsu15I ATCGAT 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 974
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 15, 820
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 2 cut(s) 472, 992
BtsCI GGATG 3 cut(s) 226, 526, 676
BtsIMutI CAGTG 1 cut(s) 1109
Cac8I GCNNGC 5 cut(s) 285, 822, 1026, 1650, 1749
CaiI CAGNNNCTG 1 cut(s) 1559
Cfr13I GGNCC 2 cut(s) 794, 989
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 4 cut(s) 99, 1223, 1388, 1592
Csp6I GTAC 5 cut(s) 215, 574, 674, 778, 948
CviAII CATG 9 cut(s) 271, 473, 578, 688, 730, 945, 951, 993, 1025
CviQI GTAC 5 cut(s) 215, 574, 674, 778, 948
DdeI CTNAG 3 cut(s) 759, 974, 1386
Eam1104I CTCTTC 2 cut(s) 72, 426
EarI CTCTTC 2 cut(s) 72, 426
EciI GGCGGA 2 cut(s) 954, 1577
Ecl136II GAGCTC 2 cut(s) 32, 599
Eco130I CCWWGG 2 cut(s) 472, 992
Eco147I AGGCCT 1 cut(s) 15
Eco24I GRGCYC 2 cut(s) 34, 601
Eco31I GGTCTC 2 cut(s) 327, 1816
Eco32I GATATC 1 cut(s) 1437
Eco47I GGWCC 2 cut(s) 794, 989
Eco53kI GAGCTC 2 cut(s) 32, 599
Eco57I CTGAAG 5 cut(s) 96, 981, 1133, 1581, 1592
Eco81I CCTNAGG 1 cut(s) 974
Eco88I CYCGRG 1 cut(s) 1724
EcoICRI GAGCTC 2 cut(s) 32, 599
EcoRII CCWGG 3 cut(s) 796, 933, 1271
EcoRV GATATC 1 cut(s) 1437
EcoT14I CCWWGG 2 cut(s) 472, 992
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 2 cut(s) 34, 601
ErhI CCWWGG 2 cut(s) 472, 992
FaeI CATG 9 cut(s) 274, 476, 581, 691, 733, 948, 954, 996, 1028
FalI AAGNNNNNCTT 4 cut(s) 360, 392, 1394, 1426
FatI CATG 9 cut(s) 270, 472, 577, 687, 729, 944, 950, 992, 1024
FbaI TGATCA 2 cut(s) 1297, 1446
Fnu4HI GCNGC 8 cut(s) 633, 711, 825, 1029, 1383, 1653, 1745, 1752
FokI GGATG 3 cut(s) 233, 513, 683
FriOI GRGCYC 2 cut(s) 34, 601
Fsp4HI GCNGC 8 cut(s) 633, 711, 825, 1029, 1383, 1653, 1745, 1752
FspBI CTAG 6 cut(s) 330, 702, 1059, 1616, 1769, 1779
GluI GCNGC 8 cut(s) 633, 711, 825, 1029, 1383, 1653, 1745, 1752
HaeIII GGCC 2 cut(s) 15, 820
HapII CCGG 2 cut(s) 187, 358
HgaI GACGC 4 cut(s) 99, 1223, 1388, 1592
Hin1II CATG 9 cut(s) 274, 476, 581, 691, 733, 948, 954, 996, 1028
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 8 cut(s) 261, 763, 911, 1088, 1145, 1570, 1688, 1802
HpaII CCGG 2 cut(s) 187, 358
HphI GGTGA 2 cut(s) 541, 1082
Hpy166II GTNNAC 4 cut(s) 144, 502, 1073, 1598
Hpy188I TCNGA 7 cut(s) 47, 61, 76, 1156, 1326, 1357, 1451
Hpy188III TCNNGA 9 cut(s) 35, 96, 112, 839, 1006, 1319, 1444, 1574, 1724
Hpy8I GTNNAC 4 cut(s) 144, 502, 1073, 1598
HpyAV CCTTC 7 cut(s) 14, 112, 956, 1108, 1182, 1352, 1488
HpyCH4III ACNGT 1 cut(s) 1827
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 289, 1034, 1609
HpyF3I CTNAG 3 cut(s) 759, 974, 1386
Hsp92II CATG 9 cut(s) 274, 476, 581, 691, 733, 948, 954, 996, 1028
Ksp22I TGATCA 2 cut(s) 1297, 1446
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 3 cut(s) 29, 1633, 1672
Lsp1109I GCAGC 8 cut(s) 644, 697, 811, 1015, 1394, 1639, 1731, 1738
LweI GCATC 2 cut(s) 211, 801
MaeI CTAG 6 cut(s) 330, 702, 1059, 1616, 1769, 1779
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 3 cut(s) 56, 874, 1237
MhlI GDGCHC 2 cut(s) 34, 601
MlyI GAGTC 4 cut(s) 255, 757, 1579, 1682
MmeI TCCRAC 1 cut(s) 1767
Mph1103I ATGCAT 1 cut(s) 1249
MroXI GAANNNNTTC 2 cut(s) 695, 1192
MseI TTAA 8 cut(s) 156, 339, 378, 569, 804, 1349, 1638, 1704
MslI CAYNNNNRTG 2 cut(s) 949, 1418
MspA1I CMGCKG 2 cut(s) 959, 1385
MspI CCGG 2 cut(s) 187, 358
MspR9I CCNGG 4 cut(s) 188, 798, 935, 1273
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 2 cut(s) 1247, 1683
MvaI CCWGG 3 cut(s) 798, 935, 1273
MwoI GCNNNNNNNGC 4 cut(s) 128, 289, 1034, 1609
NciI CCSGG 1 cut(s) 188
NcoI CCATGG 2 cut(s) 472, 992
NlaIII CATG 9 cut(s) 274, 476, 581, 691, 733, 948, 954, 996, 1028
NlaIV GGNNCC 2 cut(s) 192, 876
NsiI ATGCAT 1 cut(s) 1249
NspI RCATGY 2 cut(s) 954, 1028
OliI CACNNNNGTG 1 cut(s) 1418
PaeI GCATGC 1 cut(s) 1028
PaeR7I CTCGAG 1 cut(s) 1724
PasI CCCWGGG 1 cut(s) 1272
PceI AGGCCT 1 cut(s) 15
PciI ACATGT 1 cut(s) 950
PciSI GCTCTTC 1 cut(s) 426
PctI GAATGC 2 cut(s) 1247, 1683
PdmI GAANNNNTTC 2 cut(s) 695, 1192
PfeI GAWTC 4 cut(s) 911, 1088, 1145, 1802
PflFI GACNNNGTC 1 cut(s) 1583
PkrI GCNGC 8 cut(s) 634, 712, 826, 1030, 1384, 1654, 1746, 1753
PleI GAGTC 4 cut(s) 255, 757, 1578, 1682
PpsI GAGTC 4 cut(s) 255, 757, 1578, 1682
PscI ACATGT 1 cut(s) 950
Psp124BI GAGCTC 2 cut(s) 34, 601
Psp6I CCWGG 3 cut(s) 796, 933, 1271
PspGI CCWGG 3 cut(s) 796, 933, 1271
PspN4I GGNNCC 2 cut(s) 192, 876
PspPI GGNCC 2 cut(s) 794, 989
PsrI GAACNNNNNNTAC 4 cut(s) 1114, 1146, 1493, 1525
PstI CTGCAG 2 cut(s) 606, 1756
PstNI CAGNNNCTG 1 cut(s) 1559
PsuI RGATCY 3 cut(s) 56, 874, 1237
PsyI GACNNNGTC 1 cut(s) 1583
PvuII CAGCTG 2 cut(s) 959, 1385
RsaI GTAC 5 cut(s) 216, 575, 675, 779, 949
RsaNI GTAC 5 cut(s) 215, 574, 674, 778, 948
RseI CAYNNNNRTG 2 cut(s) 949, 1418
SacI GAGCTC 2 cut(s) 34, 601
SapI GCTCTTC 1 cut(s) 426
SaqAI TTAA 8 cut(s) 156, 339, 378, 569, 804, 1349, 1638, 1704
SatI GCNGC 8 cut(s) 633, 711, 825, 1029, 1383, 1653, 1745, 1752
Sau96I GGNCC 2 cut(s) 794, 989
SchI GAGTC 4 cut(s) 255, 757, 1579, 1682
ScrFI CCNGG 4 cut(s) 188, 798, 935, 1273
SduI GDGCHC 2 cut(s) 34, 601
SfaNI GCATC 2 cut(s) 211, 801
SfcI CTRYAG 4 cut(s) 602, 1252, 1752, 1796
Sfr274I CTCGAG 1 cut(s) 1724
SinI GGWCC 2 cut(s) 794, 989
SlaI CTCGAG 1 cut(s) 1724
SmiMI CAYNNNNRTG 2 cut(s) 949, 1418
SmlI CTYRAG 5 cut(s) 35, 94, 110, 462, 1724
SmoI CTYRAG 5 cut(s) 35, 94, 110, 462, 1724
SphI GCATGC 1 cut(s) 1028
SseBI AGGCCT 1 cut(s) 15
SsiI CCGC 2 cut(s) 939, 1588
SspI AATATT 1 cut(s) 455
SspMI CTAG 6 cut(s) 330, 702, 1059, 1616, 1769, 1779
SstI GAGCTC 2 cut(s) 34, 601
StuI AGGCCT 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 186, 796, 933, 1271
StyI CCWWGG 2 cut(s) 472, 992
TaaI ACNGT 1 cut(s) 1827
TaqI TCGA 6 cut(s) 5, 180, 1005, 1517, 1691, 1725
TatI WGTACW 3 cut(s) 673, 777, 947
TfiI GAWTC 4 cut(s) 911, 1088, 1145, 1802
Tru1I TTAA 8 cut(s) 156, 339, 378, 569, 804, 1349, 1638, 1704
Tru9I TTAA 8 cut(s) 156, 339, 378, 569, 804, 1349, 1638, 1704
TscAI CASTG 1 cut(s) 1116
TseI GCWGC 8 cut(s) 632, 710, 824, 1028, 1382, 1652, 1744, 1751
TspDTI ATGAA 6 cut(s) 456, 704, 1028, 1080, 1239, 1802
TspGWI ACGGA 1 cut(s) 1421
TspRI CASTG 1 cut(s) 1116
Tth111I GACNNNGTC 1 cut(s) 1583
VpaK11BI GGWCC 2 cut(s) 794, 989
XceI RCATGY 2 cut(s) 954, 1028
XhoI CTCGAG 1 cut(s) 1724
XmnI GAANNNNTTC 2 cut(s) 695, 1192
XspI CTAG 6 cut(s) 330, 702, 1059, 1616, 1769, 1779
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.