Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0021906

Physical Location & Seq

Genomic Coordinates Forward (+)

5160192 .. 5160683

Gene Structure 492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1064400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 492 bp

ATGGCATCCGAAAACTCACAACTCTTTGAGCGGCATTTCAGTGATCTCAACTACTCTGACTTTCACACCTACAGCCAAGGCCACTTACTAGTCCTCTTCTTCTCTCTGATCTTCATCATCGCCACCCTATTCTTCTATGCTCATTGGGCTTGCATCAAAGGTCGTCTCTCAACAGCCACCGCTAGTGCTACAAATATTGCATCTTTGCCACAAGTGCATCAGTCGCTAGGGCTCGATGCTGCTGCCATTGATAGCTTGCCGGTTATCTTGCATCGATCGGTGGTAATAGCTAACCCTAGTACTGTTCTTGATGTTGAAGCCATTGGTATCGAGTTTTGCATTTGTCTTGGAGTTTTTGAAGGAGAAGAGAAGGTTAAGGTGTTGCCAAAGTGCCAAGATACATATCATTCTGAGTATGTAGATAAGTGGCTGACCGTTCAGTCAAGCTGCCCGCTTTGTAGATTATCCTTCCGAGTCATCAACTCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

164

Amino Acids

18.17

Weight (kDa)

5.72

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	113 - 154	6e-09	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0021906)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11110
malus_domestica	MD03G1064400.v1.1 MD11G1068200.v1.1
prunus_persica	Prupe.6G053600_v2.0.a1

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
AccBSI	CCGCTC	1 cut(s)	31
AciI	CCGC	3 cut(s)	31, 180, 452
AfaI	GTAC	1 cut(s)	301
AgsI	TTSAA	2 cut(s)	317, 359
AhlI	ACTAGT	1 cut(s)	88
AluBI	AGCT	3 cut(s)	255, 290, 447
AluI	AGCT	3 cut(s)	255, 290, 447
Alw26I	GTCTC	1 cut(s)	170
AoxI	GGCC	1 cut(s)	79
ApeKI	GCWGC	3 cut(s)	239, 242, 447
BanII	GRGCYC	1 cut(s)	234
BbvI	GCAGC	3 cut(s)	226, 229, 434
BcgI	CGANNNNNNTGC	2 cut(s)	224, 258
BcoDI	GTCTC	1 cut(s)	170
BcuI	ACTAGT	1 cut(s)	88
BfaI	CTAG	4 cut(s)	89, 183, 227, 297
BfmI	CTRYAG	1 cut(s)	70
BisI	GCNGC	4 cut(s)	32, 240, 243, 448
BlsI	GCNGC	4 cut(s)	33, 241, 244, 449
BmcAI	AGTACT	1 cut(s)	301
BmsI	GCATC	6 cut(s)	14, 162, 209, 226, 226, 280
Bsa29I	ATCGAT	1 cut(s)	274
BsaBI	GATNNNNATC	2 cut(s)	113, 402
BsaJI	CCNNGG	1 cut(s)	76
Bse118I	RCCGGY	1 cut(s)	259
Bse8I	GATNNNNATC	2 cut(s)	113, 402
BseCI	ATCGAT	1 cut(s)	274
BseDI	CCNNGG	1 cut(s)	76
BseGI	GGATG	1 cut(s)	5
BseJI	GATNNNNATC	2 cut(s)	113, 402
BseMII	CTCAG	1 cut(s)	402
BseXI	GCAGC	3 cut(s)	226, 229, 434
Bsh1285I	CGRYCG	1 cut(s)	278
BshFI	GGCC	1 cut(s)	81
BshVI	ATCGAT	1 cut(s)	274
BsiEI	CGRYCG	1 cut(s)	278
BsiSI	CCGG	1 cut(s)	260
BsmAI	GTCTC	1 cut(s)	170
BsmBI	CGTCTC	1 cut(s)	170
BsnI	GGCC	1 cut(s)	81
Bsp1286I	GDGCHC	1 cut(s)	234
Bsp143I	GATC	3 cut(s)	43, 108, 275
BspACI	CCGC	3 cut(s)	31, 180, 452
BspANI	GGCC	1 cut(s)	81
BspCNI	CTCAG	1 cut(s)	403
BspDI	ATCGAT	1 cut(s)	274
BsrBI	CCGCTC	1 cut(s)	31
BsrFI	RCCGGY	1 cut(s)	259
BssAI	RCCGGY	1 cut(s)	259
BssECI	CCNNGG	1 cut(s)	76
BssMI	GATC	3 cut(s)	43, 108, 275
BssT1I	CCWWGG	1 cut(s)	76
Bst4CI	ACNGT	2 cut(s)	304, 436
Bst6I	CTCTTC	2 cut(s)	101, 360
BstC8I	GCNNGC	3 cut(s)	151, 257, 452
BstDEI	CTNAG	1 cut(s)	411
BstF5I	GGATG	1 cut(s)	5
BstKTI	GATC	3 cut(s)	46, 111, 278
BstMAI	GTCTC	1 cut(s)	170
BstMBI	GATC	3 cut(s)	43, 108, 275
BstMCI	CGRYCG	1 cut(s)	278
BstMWI	GCNNNNNNNGC	3 cut(s)	146, 214, 223
BstSFI	CTRYAG	1 cut(s)	70
BstV1I	GCAGC	3 cut(s)	226, 229, 434
Bsu15I	ATCGAT	1 cut(s)	274
BsuRI	GGCC	1 cut(s)	81
BsuTUI	ATCGAT	1 cut(s)	274
BtgZI	GCGATG	1 cut(s)	103
BtsCI	GGATG	1 cut(s)	5
BtsIMutI	CAGTG	1 cut(s)	46
Cac8I	GCNNGC	3 cut(s)	151, 257, 452
Cfr10I	RCCGGY	1 cut(s)	259
ClaI	ATCGAT	1 cut(s)	274
Csp6I	GTAC	1 cut(s)	300
CviQI	GTAC	1 cut(s)	300
DdeI	CTNAG	1 cut(s)	411
DpnI	GATC	3 cut(s)	45, 110, 277
DpnII	GATC	3 cut(s)	43, 108, 275
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
Eam1104I	CTCTTC	2 cut(s)	101, 360
EarI	CTCTTC	2 cut(s)	101, 360
Eco130I	CCWWGG	1 cut(s)	76
Eco24I	GRGCYC	1 cut(s)	234
EcoT14I	CCWWGG	1 cut(s)	76
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	76
Esp3I	CGTCTC	1 cut(s)	170
FaiI	YATR	3 cut(s)	138, 403, 417
FauI	CCCGC	1 cut(s)	459
Fnu4HI	GCNGC	4 cut(s)	32, 240, 243, 448
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	4 cut(s)	32, 240, 243, 448
FspBI	CTAG	4 cut(s)	89, 183, 227, 297
GluI	GCNGC	4 cut(s)	32, 240, 243, 448
HaeIII	GGCC	1 cut(s)	81
HapII	CCGG	1 cut(s)	260
HinfI	GANTC	1 cut(s)	474
HpaII	CCGG	1 cut(s)	260
Hpy188I	TCNGA	5 cut(s)	10, 58, 108, 412, 473
Hpy188III	TCNNGA	1 cut(s)	308
HpyAV	CCTTC	3 cut(s)	353, 364, 478
HpyCH4III	ACNGT	2 cut(s)	304, 436
HpyCH4V	TGCA	5 cut(s)	153, 200, 217, 271, 339
HpyF10VI	GCNNNNNNNGC	3 cut(s)	146, 214, 223
HpyF3I	CTNAG	1 cut(s)	411
Kzo9I	GATC	3 cut(s)	43, 108, 275
LpnPI	CCDG	1 cut(s)	273
Lsp1109I	GCAGC	3 cut(s)	226, 229, 434
LweI	GCATC	6 cut(s)	14, 162, 209, 226, 226, 280
MaeI	CTAG	4 cut(s)	89, 183, 227, 297
MalI	GATC	3 cut(s)	45, 110, 277
MbiI	CCGCTC	1 cut(s)	31
MboI	GATC	3 cut(s)	43, 108, 275
MboII	GAAGA	5 cut(s)	88, 91, 103, 124, 377
MhlI	GDGCHC	1 cut(s)	234
MlyI	GAGTC	1 cut(s)	483
MnlI	CCTC	1 cut(s)	104
MseI	TTAA	1 cut(s)	375
MslI	CAYNNNNRTG	1 cut(s)	39
MspI	CCGG	1 cut(s)	260
MwoI	GCNNNNNNNGC	3 cut(s)	146, 214, 223
NdeII	GATC	3 cut(s)	43, 108, 275
PkrI	GCNGC	4 cut(s)	33, 241, 244, 449
Ple19I	CGATCG	1 cut(s)	278
PleI	GAGTC	1 cut(s)	482
PpsI	GAGTC	1 cut(s)	482
PvuI	CGATCG	1 cut(s)	278
RsaI	GTAC	1 cut(s)	301
RsaNI	GTAC	1 cut(s)	300
RseI	CAYNNNNRTG	1 cut(s)	39
SaqAI	TTAA	1 cut(s)	375
SatI	GCNGC	4 cut(s)	32, 240, 243, 448
Sau3AI	GATC	3 cut(s)	43, 108, 275
ScaI	AGTACT	1 cut(s)	301
SchI	GAGTC	1 cut(s)	483
SduI	GDGCHC	1 cut(s)	234
SetI	ASST	7 cut(s)	71, 163, 257, 292, 375, 381, 449
SfaNI	GCATC	6 cut(s)	14, 162, 209, 226, 226, 280
SfcI	CTRYAG	1 cut(s)	70
SmiMI	CAYNNNNRTG	1 cut(s)	39
SpeI	ACTAGT	1 cut(s)	88
SsiI	CCGC	3 cut(s)	31, 180, 452
SspI	AATATT	1 cut(s)	196
SspMI	CTAG	4 cut(s)	89, 183, 227, 297
StyI	CCWWGG	1 cut(s)	76
TaaI	ACNGT	2 cut(s)	304, 436
TaqI	TCGA	3 cut(s)	234, 274, 330
TatI	WGTACW	1 cut(s)	299
TauI	GCSGC	1 cut(s)	34
Tru1I	TTAA	1 cut(s)	375
Tru9I	TTAA	1 cut(s)	375
TscAI	CASTG	1 cut(s)	46
TseI	GCWGC	3 cut(s)	239, 242, 447
TspDTI	ATGAA	1 cut(s)	103
TspRI	CASTG	1 cut(s)	46
XspI	CTAG	4 cut(s)	89, 183, 227, 297
ZrmI	AGTACT	1 cut(s)	301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.