Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007391

Physical Location & Seq

Genomic Coordinates Reverse (-)

5848221 .. 5855049

Gene Structure 6829 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1071900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2781 bp

ATGTCTGTTTACGGTAAAGACACGGATCCAGCATTCCAGGCTGCAGGAACAAATCTTGGTTTGGAAATTTGGTGTGTTGAGAACCTAAAATTGGTTTCTGTTCCAAAATCTTCGCATCGGAAATTCTATTCCGGAAGTGCATATGTCATTTTAAATACATTTCTGCCGAAAAATGGCCTTCCTCAGCATGACATACACTATTGGCTGGGAAATGACTCAAACAAGGAGGACTCGGCGTTGGCATCAGATAAAGCACTAGAACTAGATGCAGCCCTAGGGTCCTGCACTGTGCAATATAGGGAGCTTCAAGGCCATGAAACACAAAAGTTTTTATCGTACTTCAAACCCTGTATTATCCCCATTGAAGGAGCATATAGAACACAACAAGAGCGCTTAAATGGTGAAACATATAAAGTCAGCTTGTTAGCTTGCAAAGGGGACCATGTTGTTCATGTTAAAGAAGTTCCTTTCTCTCGGTCATCATTGAACCACAATGACGTATTCATACTTGATACTGCATCGAAAATTTTCCTCTTTAGTGGGTGCAACTCTAGCATACAGGAAAGAGCCAAGGGTTTGGAGGTTGTTCAATACATCAAAGAGAATAAGCATCGGGAAAAATGCGAGGTGGCAACTGTAGAGGATGGAAAATTCGTTGGTGATCCTGAAGTGGGTGAATTCTGGAGCTTATTTGGTGGTTATGCTCCCATTCCCCAGGATCCACCGTCTTCTGTCCAGGAAAGACCGGAGACACCCTTTGTGAAACTATCGTGTTTTGTTGACATTATTCTAAGGATATCAACTCAGGGTAAACTATGTCCATGTCGAACTGACTCACTAAATAAAGAAATGTTGGAGACGGACAAGTGTTATATGCTGGACTGTGATTCTGAGATTTTTGTTTGGATGGGAAAGCATACCTCAGTTACAGAAAGAAAAACATCAATTTCCGCTACAGAAGATTTCCTCAGAAACCAAGGCAGGTCAGCGGGGACTCATTCAGTGTTTATAACTGAAGGATTAGAAACTGTTAAGTTTAGGTCATACTTTGATAATTGGCCTCAAACAGTCAAAATCAAGCTGTATGAAGAAGGCCGAGAAAAAGTAGCAGCAATGTTCAAGCAAAAGGGTTATGAGGTGAAAGAGCTTCCAGATGAAGAAGACATCCAGCCATTTATAGATTGCAGTGGCACATTGAAGGTTTGGCGGGTGGATGGTGATAAACTGTCCCTCATTCCAGCTTCAGACCAGCGGAAAATATTCAGCGGGGATTGCTATGTTGTGCAGTATACTTATCCTGGAAAGGAAAAGAGTGACCATCTCTTTTATGCATGGCTTGGCTGTCAAAGCGTAACGGAAGATCGAAGGGATGCTATCTCTCGTATGAATGCCCTAGTCGATTCAACCAAGGGAGACCCTGTTTTGGCTCAAGTTATGGAGAACAAGGAGCCTTCTCAATTCTTTTCCATTTTCCGGACATTAATTATTTACAAGGGAGGTAGAAGTGCACGATACAAGAATTTTATAGCAGAAAATGGTATTGCAGATGAAACTTATGATGAAAGCAAGACTGCCCTTTTTCGTGTTCAAGGGACAAGTCCCAACAATATGCAGGCGATCCAAGTTGATCCGGTTTCAAGATCTTTGAACTCGTGTTATTGTTACATCCTGCAAACTGGTGCATCTGCTTTCACTTGGATTGGGAATCTCTCCTCGACCAGAGACCATGACCTTCTTGACAGAATGCTCGAATTAATACATCCAACGTGGCAAGCCGTATCAGTGAGGGAAGGGAGTGAATCCGATGCTTTCTGGAATGTGCTTGGTGGAAAGGCTGAGTATCCGAGGGCAAAAGAAATAAAAGGACACATGGAAGACCCAAATTTGTTTATGTTGAGCATAACTGAAGATACTTCATCTGAATCAGTTTGCATATTTCAGGTGAAGGAGATATACAATTTTACACAGGATGATTTAACTACTGAAGACGTGTTATTACTTGACTGCCACGACGAGATTTATGTTTGGATTGGATGCCACTCTAAGATTAGTTCAAAGCAACAAGCCCTTACTCTAGGCCTGAAATTTCTCGAGACAGATGTACTGGTTGAAGGGCTATCTTTGGAGACTCCTATACATGTTGTTGGTGAAGGGCATGAACCACCATTTTTCACTCGTTTCTTCGAATGGGATCCATCAAAGTCAAATATGCATGGCAACTCATTTGAGCGGAAGCTAGCTATTTTAAAGGGAAAACCACAACAATTAGAAGCACCTATAAGAAACACATGGAAAGCAAACTCCAGGTCCAGGGAGACAACCCCAGATGGTTTTAGAAGCAAGTCCATGAGTTCCAATGGCCGGAGAAGTGCTTCTCCTGCATCCGGTGGTTCACTTTCTAATGTCAAGTCCCCAAACAATCACATCTTTTCCAGCTCATCTGCTCCAACTAAGAAACTCTTTCCAAAATCCTCTCCTTACAGTAGTCCTGGCGAGTCAATTGTACCTGCAGGTTCTCCAGTAAACTTACTGGATAATACGGGTGGCACTAAGGCTAATGGAAGTAAGCCTGGTGAAGGAGGCCTAAACTTGTTGACGTATCCATATGAACGACTGAAAGTGGTTTCTTTAAACCCGGCAGCAGGAATAGACATAACCAAAAGAGAGGCATATCTCTCTGATGAAGAATTCCAAGTGCATTTTGGAATGGCCAAAAGGGACTTCTATAAGCTTCCTAAATGGAAACAAAACAAGCAAAAGATGGCACTTTATCTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010639 GO:0015629 GO:0016043 GO:0030029 GO:0030036 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051129 GO:0051493 GO:0051494 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904

KEGG Pathways

Metabolic & Signaling

ko04666 ko04810 ko05203 map04666 map04810 map05203

Pfam Domains

Protein Families

Gelsolin VHP

Protein Analysis

927

Amino Acids

104.03

Weight (kDa)

6.03

Isoelectric Point (pI)

42.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gelsolin	PF00626	30 - 111	5.2e-12	Gelsolin repeat
Gelsolin	PF00626	149 - 217	1.6e-11	Gelsolin repeat
Gelsolin	PF00626	279 - 323	1.4e-07	Gelsolin repeat
Gelsolin	PF00626	650 - 724	2.3e-09	Gelsolin repeat
VHP	PF02209	891 - 926	8.9e-15	Villin headpiece domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007391)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29890 AT2G29890 AT2G29890 AT2G29890 AT2G29890
fragaria_vesca	FvH4_3g38450 FvH4_3g38450 FvH4_3g38450 FvH4_3g38450 FvH4_3g38450 FvH4_3g38450 FvH4_3g38450
malus_domestica	MD03G1071900.v1.1
prunus_persica	Prupe.6G058500_v2.0.a1
pyrus_communis	pycom03g05810
rosa_chinensis	RchiOBHm_Chr5g0069141
rosa_laevigata	RLG00000036039
rosa_multiflora	Rmu_sc0000936.1_g000012
rosa_roxburghii	Rroxscaffold_1G00011650
rosa_rugosa	Rorug05G0396200
rosa_samantha	Rh5BG472200 Rh5CG494600 Rh5DG484600
rosa_wichuraiana	Rw0G006050 Rw5G042310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1010
Acc36I	ACCTGC	3 cut(s)	972, 2501, 2515
AccBSI	CCGCTC	1 cut(s)	2230
AccI	GTMKAC	1 cut(s)	1289
AccIII	TCCGGA	2 cut(s)	131, 1473
AciI	CCGC	6 cut(s)	951, 989, 1207, 1252, 1266, 2230
AclWI	GGATC	9 cut(s)	20, 33, 656, 713, 726, 1612, 1622, 2186, 2199
AcoI	YGGCCR	2 cut(s)	2359, 2709
AcsI	RAATTY	9 cut(s)	66, 122, 525, 650, 677, 1519, 1882, 2084, 2687
AcuI	CTGAAG	5 cut(s)	687, 1035, 1227, 1926, 2004
AfaI	GTAC	3 cut(s)	338, 2103, 2505
AfeI	AGCGCT	1 cut(s)	392
AflIII	ACRYGT	2 cut(s)	1989, 2137
AjiI	CACGTC	1 cut(s)	1990
AjnI	CCWGG	8 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569
AjuI	GAANNNNNNNTTGG	4 cut(s)	74, 106, 2705, 2737
Alw21I	GWGCWC	1 cut(s)	1510
Alw26I	GTCTC	7 cut(s)	743, 851, 1407, 1716, 2087, 2120, 2309
Alw44I	GTGCAC	1 cut(s)	1506
AlwI	GGATC	9 cut(s)	20, 33, 656, 713, 726, 1612, 1622, 2186, 2199
Ama87I	CYCGRG	1 cut(s)	2090
Aor13HI	TCCGGA	2 cut(s)	131, 1473
Aor51HI	AGCGCT	1 cut(s)	392
AoxI	GGCC	8 cut(s)	175, 310, 1058, 1093, 2077, 2359, 2581, 2709
ApaLI	GTGCAC	1 cut(s)	1506
ApeKI	GCWGC	4 cut(s)	41, 269, 1109, 2639
ApoI	RAATTY	9 cut(s)	66, 122, 525, 650, 677, 1519, 1882, 2084, 2687
ArsI	GACNNNNNNTTYG	2 cut(s)	2291, 2323
AseI	ATTAAT	2 cut(s)	1481, 1754
Asp700I	GAANNNNTTC	3 cut(s)	527, 1259, 2371
AspA2I	CCTAGG	1 cut(s)	274
AspLEI	GCGC	1 cut(s)	393
AspS9I	GGNCC	3 cut(s)	279, 439, 2307
AsuC2I	CCSGG	1 cut(s)	2636
AsuHPI	GGTGA	8 cut(s)	413, 671, 686, 1150, 1229, 1954, 2159, 2585
AsuII	TTCGAA	1 cut(s)	2184
AsuNHI	GCTAGC	1 cut(s)	2236
AvaI	CYCGRG	1 cut(s)	2090
AvaII	GGWCC	3 cut(s)	279, 439, 2307
AvrII	CCTAGG	1 cut(s)	274
BaeGI	GKGCMC	1 cut(s)	1510
BalI	TGGCCA	1 cut(s)	2711
BamHI	GGATCC	3 cut(s)	25, 718, 2191
BarI	GAAGNNNNNNTAC	2 cut(s)	1937, 1969
BauI	CACGAG	1 cut(s)	1651
BbsI	GAAGAC	4 cut(s)	720, 1167, 1881, 1992
Bbv12I	GWGCWC	1 cut(s)	1510
BbvCI	CCTCAGC	1 cut(s)	183
BbvI	GCAGC	4 cut(s)	28, 281, 1121, 2651
BccI	CCATC	7 cut(s)	638, 901, 1208, 1326, 2203, 2321, 2755
BceAI	ACGGC	1 cut(s)	1760
BciT130I	CCWGG	8 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571
BciVI	GTATCC	2 cut(s)	1851, 2610
BcnI	CCSGG	1 cut(s)	2636
BcoDI	GTCTC	7 cut(s)	743, 851, 1407, 1716, 2087, 2120, 2309
BfaI	CTAG	7 cut(s)	257, 263, 275, 552, 1394, 2075, 2237
BfmI	CTRYAG	4 cut(s)	42, 636, 954, 2508
BfoI	RGCGCY	1 cut(s)	394
BfuAI	ACCTGC	3 cut(s)	972, 2501, 2515
BfuI	GTATCC	2 cut(s)	1851, 2610
BglII	AGATCT	1 cut(s)	1640
BisI	GCNGC	4 cut(s)	42, 270, 1110, 2640
BlnI	CCTAGG	1 cut(s)	274
BlsI	GCNGC	4 cut(s)	43, 271, 1111, 2641
Bme1390I	CCNGG	9 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571, 2636
Bme18I	GGWCC	3 cut(s)	279, 439, 2307
BmeT110I	CYCGRG	1 cut(s)	2090
BmgBI	CACGTC	1 cut(s)	1990
BmgT120I	GGNCC	3 cut(s)	279, 439, 2307
BmiI	GGNNCC	6 cut(s)	27, 280, 440, 720, 1449, 2193
BmrFI	CCNGG	9 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571, 2636
BmtI	GCTAGC	1 cut(s)	2240
BpiI	GAAGAC	4 cut(s)	720, 1167, 1881, 1992
BpmI	CTGGAG	3 cut(s)	703, 2287, 2502
Bpu10I	CCTNAGC	1 cut(s)	183
Bpu14I	TTCGAA	1 cut(s)	2184
BpuEI	CTTGAG	1 cut(s)	1413
BpuMI	CCSGG	1 cut(s)	2636
BsaI	GGTCTC	2 cut(s)	1407, 1716
BsaJI	CCNNGG	7 cut(s)	274, 570, 714, 976, 1407, 1844, 2310
BsaWI	WCCGGW	5 cut(s)	131, 745, 1473, 1630, 2384
BsaXI	ACNNNNNCTCC	2 cut(s)	1404, 1434
Bse1I	ACTGG	4 cut(s)	1681, 2109, 2519, 2535
Bse3DI	GCAATG	1 cut(s)	1119
BseAI	TCCGGA	2 cut(s)	131, 1473
BseBI	CCWGG	8 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571
BseDI	CCNNGG	7 cut(s)	274, 570, 714, 976, 1407, 1844, 2310
BseMI	GCAATG	1 cut(s)	1119
BseMII	CTCAG	6 cut(s)	197, 818, 882, 936, 982, 1827
BseNI	ACTGG	4 cut(s)	1681, 2109, 2519, 2535
BseRI	GAGGAG	1 cut(s)	1702
BseSI	GKGCMC	1 cut(s)	1510
BseXI	GCAGC	4 cut(s)	28, 281, 1121, 2651
BseYI	CCCAGC	1 cut(s)	205
BsgI	GTGCAG	2 cut(s)	268, 1304
BshFI	GGCC	8 cut(s)	177, 312, 1060, 1095, 2079, 2361, 2583, 2711
BsiHKAI	GWGCWC	1 cut(s)	1510
BsiHKCI	CYCGRG	1 cut(s)	2090
BsiSI	CCGG	7 cut(s)	132, 746, 1474, 1631, 2362, 2385, 2636
BslFI	GGGAC	7 cut(s)	452, 1006, 1213, 1584, 1606, 2395, 2732
BsmAI	GTCTC	7 cut(s)	743, 851, 1407, 1716, 2087, 2120, 2309
BsmBI	CGTCTC	1 cut(s)	851
BsmFI	GGGAC	7 cut(s)	452, 1006, 1213, 1584, 1606, 2395, 2732
BsmI	GAATGC	3 cut(s)	32, 1393, 1749
BsnI	GGCC	8 cut(s)	177, 312, 1060, 1095, 2079, 2361, 2583, 2711
Bso31I	GGTCTC	2 cut(s)	1407, 1716
BsoBI	CYCGRG	1 cut(s)	2090
Bsp119I	TTCGAA	1 cut(s)	2184
Bsp1286I	GDGCHC	1 cut(s)	1510
Bsp13I	TCCGGA	2 cut(s)	131, 1473
Bsp143I	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
BspACI	CCGC	6 cut(s)	951, 989, 1207, 1252, 1266, 2230
BspANI	GGCC	8 cut(s)	177, 312, 1060, 1095, 2079, 2361, 2583, 2711
BspCNI	CTCAG	6 cut(s)	196, 817, 883, 935, 981, 1828
BspEI	TCCGGA	2 cut(s)	131, 1473
BspLI	GGNNCC	6 cut(s)	27, 280, 440, 720, 1449, 2193
BspMAI	CTGCAG	2 cut(s)	46, 2512
BspMI	ACCTGC	3 cut(s)	972, 2501, 2515
BspOI	GCTAGC	1 cut(s)	2240
BspPI	GGATC	9 cut(s)	20, 33, 656, 713, 726, 1612, 1622, 2186, 2199
BspT104I	TTCGAA	1 cut(s)	2184
BspTNI	GGTCTC	2 cut(s)	1407, 1716
BsrBI	CCGCTC	1 cut(s)	2230
BsrDI	GCAATG	1 cut(s)	1119
BsrI	ACTGG	4 cut(s)	1681, 2109, 2519, 2535
BssECI	CCNNGG	7 cut(s)	274, 570, 714, 976, 1407, 1844, 2310
BssMI	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
BssNAI	GTATAC	1 cut(s)	1290
BssSI	CACGAG	1 cut(s)	1651
BssT1I	CCWWGG	4 cut(s)	274, 570, 976, 1407
Bst1107I	GTATAC	1 cut(s)	1290
Bst2BI	CACGAG	1 cut(s)	1651
Bst2UI	CCWGG	8 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571
Bst4CI	ACNGT	9 cut(s)	14, 289, 637, 726, 884, 1030, 1069, 1227, 2483
BstBI	TTCGAA	1 cut(s)	2184
BstC8I	GCNNGC	4 cut(s)	430, 1614, 1773, 2238
BstENI	CCTNNNNNAGG	1 cut(s)	2574
BstH2I	RGCGCY	1 cut(s)	394
BstHHI	GCGC	1 cut(s)	393
BstKTI	GATC	8 cut(s)	28, 664, 721, 1363, 1620, 1630, 1643, 2194
BstMAI	GTCTC	7 cut(s)	743, 851, 1407, 1716, 2087, 2120, 2309
BstMBI	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
BstMWI	GCNNNNNNNGC	5 cut(s)	38, 552, 1272, 1347, 2378
BstNI	CCWGG	8 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571
BstNSI	RCATGY	1 cut(s)	2141
BstSCI	CCNGG	9 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569, 2634
BstSFI	CTRYAG	4 cut(s)	42, 636, 954, 2508
BstSLI	GKGCMC	1 cut(s)	1510
BstV1I	GCAGC	4 cut(s)	28, 281, 1121, 2651
BstV2I	GAAGAC	4 cut(s)	720, 1167, 1881, 1992
BstX2I	RGATCY	4 cut(s)	25, 718, 1640, 2191
BstXI	CCANNNNNNTGG	1 cut(s)	577
BstYI	RGATCY	4 cut(s)	25, 718, 1640, 2191
BstZ17I	GTATAC	1 cut(s)	1290
BsuI	GTATCC	2 cut(s)	1851, 2610
BsuRI	GGCC	8 cut(s)	177, 312, 1060, 1095, 2079, 2361, 2583, 2711
BtrI	CACGTC	1 cut(s)	1990
BtsI	GCAGTG	1 cut(s)	1192
BtsIMutI	CAGTG	4 cut(s)	285, 1008, 1192, 1788
BveI	ACCTGC	3 cut(s)	972, 2501, 2515
Cac8I	GCNNGC	4 cut(s)	430, 1614, 1773, 2238
CfoI	GCGC	1 cut(s)	393
Cfr13I	GGNCC	3 cut(s)	279, 439, 2307
Csp6I	GTAC	3 cut(s)	337, 2102, 2504
CviQI	GTAC	3 cut(s)	337, 2102, 2504
DpnI	GATC	8 cut(s)	27, 663, 720, 1362, 1619, 1629, 1642, 2193
DpnII	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
DraI	TTTAAA	3 cut(s)	153, 2247, 2631
EaeI	YGGCCR	2 cut(s)	2359, 2709
Eco130I	CCWWGG	4 cut(s)	274, 570, 976, 1407
Eco147I	AGGCCT	2 cut(s)	2079, 2583
Eco31I	GGTCTC	2 cut(s)	1407, 1716
Eco32I	GATATC	1 cut(s)	798
Eco47I	GGWCC	3 cut(s)	279, 439, 2307
Eco47III	AGCGCT	1 cut(s)	392
Eco57I	CTGAAG	5 cut(s)	687, 1035, 1227, 1926, 2004
Eco88I	CYCGRG	1 cut(s)	2090
EcoNI	CCTNNNNNAGG	1 cut(s)	2574
EcoO109I	RGGNCCY	1 cut(s)	279
EcoRI	GAATTC	2 cut(s)	677, 2687
EcoRII	CCWGG	8 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569
EcoRV	GATATC	1 cut(s)	798
EcoT14I	CCWWGG	4 cut(s)	274, 570, 976, 1407
EcoT22I	ATGCAT	2 cut(s)	1333, 2214
ErhI	CCWWGG	4 cut(s)	274, 570, 976, 1407
Esp3I	CGTCTC	1 cut(s)	851
FalI	AAGNNNNNCTT	2 cut(s)	2444, 2476
FaqI	GGGAC	7 cut(s)	452, 1006, 1213, 1584, 1606, 2395, 2732
FauI	CCCGC	3 cut(s)	982, 1200, 1259
FauNDI	CATATG	2 cut(s)	142, 2605
FblI	GTMKAC	1 cut(s)	1289
Fnu4HI	GCNGC	4 cut(s)	42, 270, 1110, 2640
Fsp4HI	GCNGC	4 cut(s)	42, 270, 1110, 2640
FspBI	CTAG	7 cut(s)	257, 263, 275, 552, 1394, 2075, 2237
GlaI	GCGC	1 cut(s)	392
GluI	GCNGC	4 cut(s)	42, 270, 1110, 2640
GsaI	CCCAGC	1 cut(s)	209
GsuI	CTGGAG	3 cut(s)	703, 2287, 2502
HaeII	RGCGCY	1 cut(s)	394
HaeIII	GGCC	8 cut(s)	177, 312, 1060, 1095, 2079, 2361, 2583, 2711
HapII	CCGG	7 cut(s)	132, 746, 1474, 1631, 2362, 2385, 2636
HhaI	GCGC	1 cut(s)	393
Hin6I	GCGC	1 cut(s)	391
HinP1I	GCGC	1 cut(s)	391
HincII	GTYRAC	2 cut(s)	781, 2595
HindII	GTYRAC	2 cut(s)	781, 2595
HindIII	AAGCTT	1 cut(s)	2729
HpaII	CCGG	7 cut(s)	132, 746, 1474, 1631, 2362, 2385, 2636
HphI	GGTGA	8 cut(s)	413, 671, 686, 1150, 1229, 1954, 2159, 2585
Hpy166II	GTNNAC	8 cut(s)	10, 781, 812, 1290, 1508, 2393, 2524, 2595
Hpy188I	TCNGA	9 cut(s)	120, 247, 892, 971, 1246, 1804, 1845, 1921, 2680
Hpy8I	GTNNAC	8 cut(s)	10, 781, 812, 1290, 1508, 2393, 2524, 2595
Hpy99I	CGWCG	1 cut(s)	2015
HpyCH4III	ACNGT	9 cut(s)	14, 289, 637, 726, 884, 1030, 1069, 1227, 2483
HpyCH4IV	ACGT	4 cut(s)	498, 1766, 1989, 2597
HpyF10VI	GCNNNNNNNGC	5 cut(s)	38, 552, 1272, 1347, 2378
HpySE526I	ACGT	4 cut(s)	498, 1766, 1989, 2597
HspAI	GCGC	1 cut(s)	391
Kpn2I	TCCGGA	2 cut(s)	131, 1473
Kzo9I	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
LmnI	GCTCC	6 cut(s)	301, 368, 684, 709, 1447, 2449
Lsp1109I	GCAGC	4 cut(s)	28, 281, 1121, 2651
MaeI	CTAG	7 cut(s)	257, 263, 275, 552, 1394, 2075, 2237
MaeII	ACGT	4 cut(s)	498, 1766, 1989, 2597
MaeIII	GTNAC	4 cut(s)	925, 1313, 1351, 1661
MalI	GATC	8 cut(s)	27, 663, 720, 1362, 1619, 1629, 1642, 2193
MbiI	CCGCTC	1 cut(s)	2230
MboI	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
MfeI	CAATTG	1 cut(s)	2499
MflI	RGATCY	4 cut(s)	25, 718, 1640, 2191
MhlI	GDGCHC	1 cut(s)	1510
MlsI	TGGCCA	1 cut(s)	2711
MluNI	TGGCCA	1 cut(s)	2711
MlyI	GAGTC	6 cut(s)	209, 224, 827, 988, 2122, 2504
MmeI	TCCRAC	3 cut(s)	834, 1787, 2471
Mox20I	TGGCCA	1 cut(s)	2711
Mph1103I	ATGCAT	2 cut(s)	1333, 2214
MroI	TCCGGA	2 cut(s)	131, 1473
MroXI	GAANNNNTTC	3 cut(s)	527, 1259, 2371
MscI	TGGCCA	1 cut(s)	2711
MseI	TTAA	9 cut(s)	152, 395, 456, 1032, 1481, 1754, 1976, 2246, 2630
Msp20I	TGGCCA	1 cut(s)	2711
MspA1I	CMGCKG	3 cut(s)	989, 1252, 1266
MspI	CCGG	7 cut(s)	132, 746, 1474, 1631, 2362, 2385, 2636
MspR9I	CCNGG	9 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571, 2636
MunI	CAATTG	1 cut(s)	2499
Mva1269I	GAATGC	3 cut(s)	32, 1393, 1749
MvaI	CCWGG	8 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571
MwoI	GCNNNNNNNGC	5 cut(s)	38, 552, 1272, 1347, 2378
NciI	CCSGG	1 cut(s)	2636
NdeI	CATATG	2 cut(s)	142, 2605
NdeII	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
NheI	GCTAGC	1 cut(s)	2236
NlaIV	GGNNCC	6 cut(s)	27, 280, 440, 720, 1449, 2193
NmeAIII	GCCGAG	2 cut(s)	212, 1121
NmuCI	GTSAC	1 cut(s)	1313
NsiI	ATGCAT	2 cut(s)	1333, 2214
NspI	RCATGY	1 cut(s)	2141
NspV	TTCGAA	1 cut(s)	2184
PaeR7I	CTCGAG	1 cut(s)	2090
PceI	AGGCCT	2 cut(s)	2079, 2583
PciI	ACATGT	1 cut(s)	2137
PctI	GAATGC	3 cut(s)	32, 1393, 1749
PdmI	GAANNNNTTC	3 cut(s)	527, 1259, 2371
PfeI	GAWTC	5 cut(s)	887, 1400, 1705, 1799, 1922
PfoI	TCCNGGA	2 cut(s)	735, 1297
PkrI	GCNGC	4 cut(s)	43, 271, 1111, 2641
PleI	GAGTC	6 cut(s)	209, 224, 827, 988, 2122, 2503
PpsI	GAGTC	6 cut(s)	209, 224, 827, 988, 2122, 2503
PpuMI	RGGWCCY	1 cut(s)	279
PscI	ACATGT	1 cut(s)	2137
PshBI	ATTAAT	2 cut(s)	1481, 1754
PsiI	TTATAA	1 cut(s)	1010
Psp5II	RGGWCCY	1 cut(s)	279
Psp6I	CCWGG	8 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569
PspFI	CCCAGC	1 cut(s)	205
PspGI	CCWGG	8 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569
PspN4I	GGNNCC	6 cut(s)	27, 280, 440, 720, 1449, 2193
PspPI	GGNCC	3 cut(s)	279, 439, 2307
PspPPI	RGGWCCY	1 cut(s)	279
PstI	CTGCAG	2 cut(s)	46, 2512
PsuI	RGATCY	4 cut(s)	25, 718, 1640, 2191
RsaI	GTAC	3 cut(s)	338, 2103, 2505
RsaNI	GTAC	3 cut(s)	337, 2102, 2504
SaqAI	TTAA	9 cut(s)	152, 395, 456, 1032, 1481, 1754, 1976, 2246, 2630
SatI	GCNGC	4 cut(s)	42, 270, 1110, 2640
Sau3AI	GATC	8 cut(s)	25, 661, 718, 1360, 1617, 1627, 1640, 2191
Sau96I	GGNCC	3 cut(s)	279, 439, 2307
SbfI	CCTGCAGG	1 cut(s)	2512
SchI	GAGTC	6 cut(s)	209, 224, 827, 988, 2122, 2504
ScrFI	CCNGG	9 cut(s)	38, 716, 737, 1299, 2305, 2311, 2490, 2571, 2636
SdaI	CCTGCAGG	1 cut(s)	2512
SduI	GDGCHC	1 cut(s)	1510
SfcI	CTRYAG	4 cut(s)	42, 636, 954, 2508
Sfr274I	CTCGAG	1 cut(s)	2090
SfuI	TTCGAA	1 cut(s)	2184
SinI	GGWCC	3 cut(s)	279, 439, 2307
SlaI	CTCGAG	1 cut(s)	2090
SmlI	CTYRAG	2 cut(s)	1428, 2090
SmoI	CTYRAG	2 cut(s)	1428, 2090
Sse8387I	CCTGCAGG	1 cut(s)	2512
SseBI	AGGCCT	2 cut(s)	2079, 2583
SsiI	CCGC	6 cut(s)	951, 989, 1207, 1252, 1266, 2230
SspI	AATATT	1 cut(s)	1260
SspMI	CTAG	7 cut(s)	257, 263, 275, 552, 1394, 2075, 2237
StuI	AGGCCT	2 cut(s)	2079, 2583
StyD4I	CCNGG	9 cut(s)	36, 714, 735, 1297, 2303, 2309, 2488, 2569, 2634
StyI	CCWWGG	4 cut(s)	274, 570, 976, 1407
TaaI	ACNGT	9 cut(s)	14, 289, 637, 726, 884, 1030, 1069, 1227, 2483
TaiI	ACGT	4 cut(s)	501, 1769, 1992, 2600
TaqI	TCGA	8 cut(s)	521, 826, 1363, 1398, 1715, 1749, 2091, 2184
TaqII	GACCGA	1 cut(s)	465
TatI	WGTACW	1 cut(s)	2101
TfiI	GAWTC	5 cut(s)	887, 1400, 1705, 1799, 1922
Tru1I	TTAA	9 cut(s)	152, 395, 456, 1032, 1481, 1754, 1976, 2246, 2630
Tru9I	TTAA	9 cut(s)	152, 395, 456, 1032, 1481, 1754, 1976, 2246, 2630
TscAI	CASTG	4 cut(s)	292, 1008, 1192, 1788
TseFI	GTSAC	1 cut(s)	1313
TseI	GCWGC	4 cut(s)	41, 269, 1109, 2639
Tsp45I	GTSAC	1 cut(s)	1313
TspGWI	ACGGA	3 cut(s)	38, 875, 1370
TspRI	CASTG	4 cut(s)	292, 1008, 1192, 1788
VneI	GTGCAC	1 cut(s)	1506
VpaK11BI	GGWCC	3 cut(s)	279, 439, 2307
VspI	ATTAAT	2 cut(s)	1481, 1754
XagI	CCTNNNNNAGG	1 cut(s)	2574
XapI	RAATTY	9 cut(s)	66, 122, 525, 650, 677, 1519, 1882, 2084, 2687
XceI	RCATGY	1 cut(s)	2141
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2699
XhoI	CTCGAG	1 cut(s)	2090
XmaJI	CCTAGG	1 cut(s)	274
XmiI	GTMKAC	1 cut(s)	1289
XmnI	GAANNNNTTC	3 cut(s)	527, 1259, 2371
XspI	CTAG	7 cut(s)	257, 263, 275, 552, 1394, 2075, 2237
Zsp2I	ATGCAT	2 cut(s)	1333, 2214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.