MD03G1101200.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019763
Physical Location & Seq
Reverse (-)
8627316 .. 8629354
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1101200.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGATGCCTTTTATGAAAATATTATCAAGGAGAGGCTCCAGGAACGGGAGACGGGAAACACTATTGGGAAGCACAAATTGGACTTGTTAGATATGTTATTGGATTATAGAAGTGACAAAGAAGACGAATTAAAAAGGTTATCCAGAAATAACATCAAGGCCATGCTTGCAGAAATGTTCATCACAGGCACAGAGACCACCTCAAGCAGTGTGGAATGGGGTATGACAGAGATCTTAAGAAACCATAATGTAGACAAGAAAGTTCTTATGGAGTTGGACCAAGTGGTTGGGAAGGATCGGTTTGTCGAAGAGAGAGACATCTCCAATCTACCTTACCTCCAAGCAGCAGTGAAAGAAGTTTTCCGGCTCCACCCAGCCTTCCCCCTGATCTTTCCTTGTAGAACAAATGAGGCGTGTGAGGTTTCCGGTTATCACATTCCCAAGGACTGTATTGTGTTCATGAATGTTTGGGGAATGGCAAGGGATCACACTATATGGGAAGAACCATGCGAGTTTAAACCAGAAAGGTTTATTGGGTCAAGCGTCGATGTCAAGGGACAAGATTTTAACCTATTGCCATTCGGGACTGGGAAAAGAATTTGTGTCGGAAAGCCACTTGGTCAACGCATGATCCACTTTTACTTGGCAGCATTGCTTCATGCTTTTGAGTGGGAATGTTCTTTGGAGATATTGGACAGCTTAGAAGAGAGAGTGGTTACGCGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko00944 ko01100 ko01110 map00941 map00944 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

243

Amino Acids

28.1

Weight (kDa)

5.37

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 227 1.2e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019763)

Species Orthologous Gene IDs
malus_domestica MD03G1101200.v1.1 MD03G1101800.v1.1 MD09G1282400.v1.1 MD09G1282500.v1.1
prunus_persica Prupe.8G008200_v2.0.a1
pyrus_communis pycom09g19040 pycom17g27110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 252
AccII CGCG 1 cut(s) 721
AclWI GGATC 3 cut(s) 303, 492, 625
AcsI RAATTY 1 cut(s) 597
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 235
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 94, 516, 548
AluBI AGCT 1 cut(s) 699
AluI AGCT 1 cut(s) 699
Alw26I GTCTC 3 cut(s) 44, 188, 309
AlwI GGATC 3 cut(s) 303, 492, 625
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 344, 647
ApoI RAATTY 1 cut(s) 597
AspS9I GGNCC 1 cut(s) 277
AvaII GGWCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 356, 659
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 3 cut(s) 44, 188, 309
BfrI CTTAAG 1 cut(s) 235
BglII AGATCT 1 cut(s) 231
BisI GCNGC 2 cut(s) 345, 648
BlsI GCNGC 2 cut(s) 346, 649
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 38, 368
BmrFI CCNGG 1 cut(s) 41
BmrI ACTGGG 1 cut(s) 597
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BpmI CTGGAG 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 441
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 592
Bse3DI GCAATG 1 cut(s) 650
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 650
BseNI ACTGG 1 cut(s) 592
BseXI GCAGC 2 cut(s) 356, 659
BseYI CCCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 721
BshFI GGCC 1 cut(s) 161
BsiSI CCGG 2 cut(s) 364, 426
BslFI GGGAC 2 cut(s) 570, 598
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 3 cut(s) 44, 188, 309
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 2 cut(s) 570, 598
BsnI GGCC 1 cut(s) 161
Bso31I GGTCTC 1 cut(s) 188
Bsp143I GATC 5 cut(s) 231, 295, 387, 484, 630
BspANI GGCC 1 cut(s) 161
BspFNI CGCG 1 cut(s) 721
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 38, 368
BspPI GGATC 3 cut(s) 303, 492, 625
BspTI CTTAAG 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 650
BsrI ACTGG 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 5 cut(s) 231, 295, 387, 484, 630
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 449
Bst6I CTCTTC 2 cut(s) 303, 699
BstAFI CTTAAG 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 721
BstKTI GATC 5 cut(s) 234, 298, 390, 487, 633
BstMAI GTCTC 3 cut(s) 44, 188, 309
BstMBI GATC 5 cut(s) 231, 295, 387, 484, 630
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 39
BstUI CGCG 1 cut(s) 721
BstV1I GCAGC 2 cut(s) 356, 659
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 161
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 2 cut(s) 214, 354
BtsIMutI CAGTG 2 cut(s) 214, 354
Cac8I GCNNGC 1 cut(s) 168
CciI TCATGA 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 277
CseI GACGC 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 5 cut(s) 163, 460, 507, 628, 659
CviJI RGCY 6 cut(s) 37, 161, 367, 377, 613, 699
CviKI_1 RGCY 6 cut(s) 37, 161, 367, 377, 613, 699
DdeI CTNAG 1 cut(s) 700
DpnI GATC 5 cut(s) 233, 297, 389, 486, 632
DpnII GATC 5 cut(s) 231, 295, 387, 484, 630
DraI TTTAAA 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 303, 699
EarI CTCTTC 2 cut(s) 303, 699
Eco130I CCWWGG 1 cut(s) 441
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 5 cut(s) 166, 463, 510, 631, 662
FaqI GGGAC 2 cut(s) 570, 598
FatI CATG 5 cut(s) 162, 459, 506, 627, 658
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 345, 648
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 345, 648
GluI GCNGC 2 cut(s) 345, 648
GsaI CCCAGC 1 cut(s) 377
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 1 cut(s) 161
HapII CCGG 2 cut(s) 364, 426
HgaI GACGC 1 cut(s) 532
Hin1II CATG 5 cut(s) 166, 463, 510, 631, 662
HincII GTYRAC 1 cut(s) 623
HindII GTYRAC 1 cut(s) 623
HinfI GANTC 1 cut(s) 723
HpaII CCGG 2 cut(s) 364, 426
Hpy166II GTNNAC 2 cut(s) 253, 623
Hpy188I TCNGA 2 cut(s) 608, 728
Hpy188III TCNNGA 3 cut(s) 144, 460, 583
Hpy8I GTNNAC 2 cut(s) 253, 623
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 286, 388
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 700
Hsp92II CATG 5 cut(s) 166, 463, 510, 631, 662
Kzo9I GATC 5 cut(s) 231, 295, 387, 484, 630
LmnI GCTCC 2 cut(s) 42, 372
Lsp1109I GCAGC 2 cut(s) 356, 659
MaeIII GTNAC 2 cut(s) 113, 715
MalI GATC 5 cut(s) 233, 297, 389, 486, 632
MboI GATC 5 cut(s) 231, 295, 387, 484, 630
MboII GAAGA 4 cut(s) 134, 320, 512, 716
MflI RGATCY 1 cut(s) 231
MluCI AATT 3 cut(s) 77, 128, 597
MmeI TCCRAC 2 cut(s) 255, 586
MnlI CCTC 5 cut(s) 27, 211, 347, 403, 412
MseI TTAA 4 cut(s) 131, 236, 516, 567
MspCI CTTAAG 1 cut(s) 235
MspI CCGG 2 cut(s) 364, 426
MspR9I CCNGG 1 cut(s) 41
MssI GTTTAAAC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 5 cut(s) 231, 295, 387, 484, 630
NlaIII CATG 5 cut(s) 166, 463, 510, 631, 662
NlaIV GGNNCC 2 cut(s) 38, 368
NmuCI GTSAC 1 cut(s) 113
PagI TCATGA 1 cut(s) 459
PfeI GAWTC 1 cut(s) 723
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 2 cut(s) 346, 649
PmeI GTTTAAAC 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 38, 368
PspPI GGNCC 1 cut(s) 277
PsuI RGATCY 1 cut(s) 231
SaqAI TTAA 4 cut(s) 131, 236, 516, 567
SatI GCNGC 2 cut(s) 345, 648
Sau3AI GATC 5 cut(s) 231, 295, 387, 484, 630
Sau96I GGNCC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 41
SetI ASST 8 cut(s) 140, 203, 334, 339, 423, 530, 573, 701
SinI GGWCC 1 cut(s) 277
SmlI CTYRAG 2 cut(s) 202, 235
SmoI CTYRAG 2 cut(s) 202, 235
Sse9I AATT 3 cut(s) 77, 128, 597
SspI AATATT 1 cut(s) 22
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 449
TaqI TCGA 2 cut(s) 306, 546
TasI AATT 3 cut(s) 77, 128, 597
TfiI GAWTC 1 cut(s) 723
Tru1I TTAA 4 cut(s) 131, 236, 516, 567
Tru9I TTAA 4 cut(s) 131, 236, 516, 567
TscAI CASTG 2 cut(s) 214, 354
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 2 cut(s) 344, 647
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 5 cut(s) 30, 169, 448, 476, 647
TspRI CASTG 2 cut(s) 214, 354
Vha464I CTTAAG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 597
XmiI GTMKAC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.