MD09G1282400.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0019763
Physical Location & Seq
Forward (+)
35952605 .. 35954565
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1282400.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGGTACACGATGGAGAGTGTTCAGAAGAATATTGAATGAAAATGTCTTCTCTTCTAGAGCCCTTGACAATTTTACACCACTTTTACAAGCAAAAGGTTCGTTAATTTGCGTCATCTTAACTTTTGCCTGCTCGATCATGTTATATTACTTTATTGAAACCATTCAAGCATCTTGGCACATACTAATTGCAGTAGTATCTGTTGATTTGATATATGAGGTCCATGGATTACTGAAGCAGCTCTACTCAGCTTCTTTGTCAAAGAATCCGGTGAACATTGCAGAATGGGTTTTCATCACCGTAGCCAACATTATAAGTAACCTTACATGCAGTAAGAGCCTTTTTGAATACACAACAAAGGAAGGGAGAGAAATGAAACAATCATTAAAAGAATTAATAAAACTTCACGGCATGGGCAATATTGCTGATTTCTTCCCATTATTCAAGCTTTTTGATCCTCAAGGGCTCAAACGGAGAACATTGGCGATCTTTCAGAAATATGATGCTTTGTATGAGAAACTTATCGACGAGAGGTTGAAAGAGAGGGAAATCGGAATCAACAATACTACTAAGAAGGAGAAAGGGGACATGTTAGATGCATTATTGAACTATAGAAGTGAAAGGCCAAGACTTCAATCTACTGCCATTTGGGTGTGGGAAAAGGATCTGTCATTTCTCCATGCCTTTGAGTGGGAATGCCCTTCTGGAGTTGTGGACAACATGGAAGAATTTGTTGGGACTACAATTCAAAAAGCCAAGAGTATCATCGCCATCCCCAAACCTAGGCTCTCAACTTCTGTTTACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

270

Amino Acids

31.04

Weight (kDa)

8.91

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 75 - 205 1e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019763)

Species Orthologous Gene IDs
malus_domestica MD03G1101200.v1.1 MD03G1101800.v1.1 MD09G1282400.v1.1 MD09G1282500.v1.1
prunus_persica Prupe.8G008200_v2.0.a1
pyrus_communis pycom09g19040 pycom17g27110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AclWI GGATC 2 cut(s) 449, 672
AcsI RAATTY 1 cut(s) 728
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 690, 783
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 9 cut(s) 37, 158, 167, 347, 445, 538, 607, 635, 749
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AluBI AGCT 3 cut(s) 241, 251, 448
AluI AGCT 3 cut(s) 241, 251, 448
AlwI GGATC 2 cut(s) 449, 672
AoxI GGCC 1 cut(s) 623
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 728
AseI ATTAAT 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 162
AspA2I CCTAGG 1 cut(s) 782
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 283, 289
AvaII GGWCC 1 cut(s) 220
AvrII CCTAGG 1 cut(s) 782
BanII GRGCYC 2 cut(s) 64, 468
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 250
BccI CCATC 2 cut(s) 6, 779
BceAI ACGGC 1 cut(s) 424
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BfaI CTAG 2 cut(s) 57, 783
BfmI CTRYAG 1 cut(s) 610
BisI GCNGC 1 cut(s) 239
BlnI CCTAGG 1 cut(s) 782
BlsI GCNGC 1 cut(s) 240
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmsI GCATC 3 cut(s) 179, 493, 586
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 223, 782
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 2 cut(s) 690, 783
Bse3DI GCAATG 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 223, 782
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 690, 783
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 250
BshFI GGCC 1 cut(s) 625
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 2 cut(s) 599, 751
BslI CCNNNNNNNGG 2 cut(s) 690, 783
BsmFI GGGAC 2 cut(s) 599, 751
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 625
Bsp1286I GDGCHC 2 cut(s) 64, 468
Bsp143I GATC 4 cut(s) 135, 454, 486, 664
Bsp19I CCATGG 1 cut(s) 223
BspANI GGCC 1 cut(s) 625
BspCNI CTCAG 1 cut(s) 260
BspPI GGATC 2 cut(s) 449, 672
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 223, 782
BssMI GATC 4 cut(s) 135, 454, 486, 664
BssT1I CCWWGG 2 cut(s) 223, 782
Bst4CI ACNGT 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 247, 570
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 771
BstKTI GATC 4 cut(s) 138, 457, 489, 667
BstMBI GATC 4 cut(s) 135, 454, 486, 664
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 330, 592
BstSFI CTRYAG 1 cut(s) 610
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 1 cut(s) 625
BtgI CCRYGG 1 cut(s) 223
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 1 cut(s) 771
Cac8I GCNNGC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 6
CviAII CATG 7 cut(s) 139, 224, 327, 412, 589, 680, 721
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 2 cut(s) 247, 570
DpnI GATC 4 cut(s) 137, 456, 488, 666
DpnII GATC 4 cut(s) 135, 454, 486, 664
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 223, 782
Eco24I GRGCYC 2 cut(s) 64, 468
Eco47I GGWCC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 254
EcoT14I CCWWGG 2 cut(s) 223, 782
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 2 cut(s) 64, 468
ErhI CCWWGG 2 cut(s) 223, 782
FaeI CATG 7 cut(s) 142, 227, 330, 415, 592, 683, 724
FaqI GGGAC 2 cut(s) 599, 751
FatI CATG 7 cut(s) 138, 223, 326, 411, 588, 679, 720
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 2 cut(s) 64, 468
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 2 cut(s) 57, 783
GluI GCNGC 1 cut(s) 239
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 1 cut(s) 625
HapII CCGG 1 cut(s) 269
HgaI GACGC 1 cut(s) 100
Hin1II CATG 7 cut(s) 142, 227, 330, 415, 592, 683, 724
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 265, 555
HpaII CCGG 1 cut(s) 269
HphI GGTGA 2 cut(s) 283, 289
Hpy166II GTNNAC 4 cut(s) 8, 274, 715, 802
Hpy188I TCNGA 3 cut(s) 26, 495, 554
Hpy188III TCNNGA 2 cut(s) 57, 705
Hpy8I GTNNAC 4 cut(s) 8, 274, 715, 802
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 3 cut(s) 356, 568, 711
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 4 cut(s) 191, 281, 330, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 247, 570
Hsp92II CATG 7 cut(s) 142, 227, 330, 415, 592, 683, 724
Kzo9I GATC 4 cut(s) 135, 454, 486, 664
LpnPI CCDG 3 cut(s) 142, 282, 690
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 3 cut(s) 179, 493, 586
MaeI CTAG 2 cut(s) 57, 783
MaeIII GTNAC 1 cut(s) 317
MalI GATC 4 cut(s) 137, 456, 488, 666
MboI GATC 4 cut(s) 135, 454, 486, 664
MboII GAAGA 5 cut(s) 39, 40, 45, 424, 737
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 2 cut(s) 64, 468
MluCI AATT 6 cut(s) 70, 105, 186, 392, 728, 744
MnlI CCTC 4 cut(s) 211, 468, 525, 537
Mph1103I ATGCAT 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 4 cut(s) 104, 119, 386, 395
MslI CAYNNNNRTG 1 cut(s) 650
MspI CCGG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 701
MwoI GCNNNNNNNGC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 223
NdeII GATC 4 cut(s) 135, 454, 486, 664
NlaIII CATG 7 cut(s) 142, 227, 330, 415, 592, 683, 724
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 2 cut(s) 330, 592
PciI ACATGT 1 cut(s) 588
PctI GAATGC 1 cut(s) 701
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 265, 555
PkrI GCNGC 1 cut(s) 240
PscI ACATGT 1 cut(s) 588
PshBI ATTAAT 1 cut(s) 395
PsiI TTATAA 1 cut(s) 314
PspPI GGNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 4 cut(s) 104, 119, 386, 395
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 4 cut(s) 135, 454, 486, 664
Sau96I GGNCC 1 cut(s) 220
SduI GDGCHC 2 cut(s) 64, 468
SetI ASST 9 cut(s) 100, 222, 243, 253, 324, 450, 536, 784, 807
SfaNI GCATC 3 cut(s) 179, 493, 586
SfcI CTRYAG 1 cut(s) 610
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 6 cut(s) 70, 105, 186, 392, 728, 744
SspI AATATT 2 cut(s) 33, 421
SspMI CTAG 2 cut(s) 57, 783
StyI CCWWGG 2 cut(s) 223, 782
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 134, 525
TasI AATT 6 cut(s) 70, 105, 186, 392, 728, 744
TfiI GAWTC 2 cut(s) 265, 555
Tru1I TTAA 4 cut(s) 104, 119, 386, 395
Tru9I TTAA 4 cut(s) 104, 119, 386, 395
TseI GCWGC 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 54, 283, 389
TspGWI ACGGA 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 220
VspI ATTAAT 1 cut(s) 395
XapI RAATTY 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 2 cut(s) 330, 592
XmaJI CCTAGG 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 2 cut(s) 57, 783
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.