Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010900

Physical Location & Seq

Genomic Coordinates Reverse (-)

12908073 .. 12913524

Gene Structure 5452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1131000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGCTCTGACTCCATGGCCGTACTTCCTCCAGCTCCCACTCCACTCCTCGCCCCACTCCGCTCAGCTCTTCTACTCCCAATCGCGAAAGGCCGAGGCCTCTCTCGTCTCTCCGATTCCAGTCAAGCTCTCCGTCTCGGTCTCGCGGAGACCCAAGTGCTTTAGATCGGCCACCGAGGAGGACCGGTTCAGCGACCCCGAAACCATCTCCGATGACGAAGCTAGACCCCACGACGAAGTTCCGGTCAACAGCCGACATCAAAGGACGACGACGGCGGCGACGAGTGGTGGCTCGGGTAGGGTTGCGATGTCGTCTGCCAGTGATTCTTTGCTCGGAATTCGCGAGCCGGTCTACGAGGTGGAAGAAGTGAAGGCTAATGGGACGATATCTGTTAGAAAAATAAACAGACGTCAGCTGCTGAAGTCAAGTGGTCTTCGTCCGCGTGATATTCGGAGTGTTGATCCTTCATTGTTTCTCACAAATTCGATGCCTTCGTTGCTGGTCCGGGAGCATGCTATTCTGTTGAATCTAGGCTCATTACGCGCAATTGCAATGCAAGAGCGTGTCCTTATATTTGACTACAACAATACAGGAAGCAGAGCTTTCATAGAAGCATTGTTGCCTCGACTAAACCCCAAAAATATGAATGGAGGACCATCTATGCCATTTGAGCTCGAGGTTGTTGAAGCAGCATTGCTCTCAAGAATACAGCGTTTAGAACAAAGACTAATGTATTTAGAACCTCGTGTTCAAAAGTTACTCAGTGTTTTGCCGAACCGGTTAACTGCTGACATACTGGAAGAGCTTCGTATTAGCAAGCAAACCTTGGTTGAATTGGGTTCGAGGGCAGGGGCTTTCAGGCAAGTGCTCTTTGATCTGTTAGAGGATCCCCATGAAATACGCCGTATATGCATTATGGGGAGAAATTGTACACTCAAGAAGATAAATGATGACATGGAATGTTCTGTTCCTTTAGAGAAGCAGATTGCTGAAGAAGAGGAGGAAGAAATTGAGATGCTCTTGGAAAATTATCTTCAACGGTGTGAATCTTGTCATGGCCAGGCTGAAAGGCTTCTCGATTCTGCTAAAGAAATGGAAGATTCTATAGCTGTCAATTTGAGTTCTCGGAGGCTTGAGGTTAGCAGGGTGGAATTACTACTCCAGGTTGGGACGTTTTGTGTGGCACTTGGCGCTCTGGTTGCAGGTATTTTTGGCATGAACTTGAGGTCCTACCTCGAAGAACATGTGTTCGCATTTTGGTTTACAACAGCAGGGATACTAGTCGGCGCCGTCTTCGGATTTTTTCTTGTCTACTCTTATCTCAGGACACGGAAAATACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006996 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009657 GO:0009668 GO:0009941 GO:0009987 GO:0010027 GO:0010117 GO:0010960 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0016043 GO:0022857 GO:0022890 GO:0030001 GO:0031967 GO:0031975 GO:0034220 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055080 GO:0055085 GO:0061024 GO:0065007 GO:0065008 GO:0070838 GO:0071840 GO:0072507 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

448

Amino Acids

50.41

Weight (kDa)

6.31

Isoelectric Point (pI)

57.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	127 - 377	6e-37	Magnesium transporter MRS2-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22830 AT5G22830
fragaria_vesca	FvH4_3g30160 FvH4_3g30160 FvH4_3g30160
malus_domestica	MD03G1131000.v1.1 MD11G1153500.v1.1
prunus_persica	Prupe.6G116100_v2.0.a1
pyrus_communis	pycom11g12530
rosa_chinensis	RchiOBHm_Chr5g0055951
rosa_laevigata	RLG00000035036
rosa_multiflora	Rmu_sc0000604.1_g000004 Rmu_sc0014108.1_g000002
rosa_roxburghii	Rroxscaffold_1G00024350
rosa_rugosa	Rorug05G0296400
rosa_samantha	Rh5AG367700 Rh5BG378000 Rh5CG401300 Rh5DG391100
rosa_wichuraiana	Rw5G034490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	412
Acc36I	ACCTGC	1 cut(s)	1193
AccB1I	GGYRCC	1 cut(s)	1286
AccBSI	CCGCTC	1 cut(s)	62
AccI	GTMKAC	2 cut(s)	351, 1311
AccII	CGCG	5 cut(s)	85, 145, 342, 442, 543
AciI	CCGC	4 cut(s)	60, 145, 275, 440
AclWI	GGATC	3 cut(s)	455, 881, 894
AcoI	YGGCCR	3 cut(s)	17, 168, 1057
AcsI	RAATTY	2 cut(s)	336, 481
AcuI	CTGAAG	2 cut(s)	440, 1011
AcyI	GRCGYC	2 cut(s)	409, 1287
AfaI	GTAC	2 cut(s)	23, 931
AflIII	ACRYGT	1 cut(s)	1243
AgeI	ACCGGT	2 cut(s)	183, 777
AgsI	TTSAA	5 cut(s)	526, 686, 752, 833, 1037
AhlI	ACTAGT	1 cut(s)	1279
AjnI	CCWGG	2 cut(s)	1059, 1161
AloI	GAACNNNNNNTCC	4 cut(s)	170, 202, 1211, 1243
AluBI	AGCT	9 cut(s)	34, 67, 127, 221, 415, 602, 673, 805, 1109
AluI	AGCT	9 cut(s)	34, 67, 127, 221, 415, 602, 673, 805, 1109
Alw21I	GWGCWC	2 cut(s)	675, 870
Alw26I	GTCTC	4 cut(s)	112, 139, 142, 145
AlwI	GGATC	3 cut(s)	455, 881, 894
AlwNI	CAGNNNCTG	1 cut(s)	418
Ama87I	CYCGRG	2 cut(s)	292, 674
AoxI	GGCC	5 cut(s)	17, 90, 96, 168, 1057
ApeKI	GCWGC	2 cut(s)	415, 689
ApoI	RAATTY	2 cut(s)	336, 481
ArsI	GACNNNNNNTTYG	2 cut(s)	228, 260
AsiGI	ACCGGT	2 cut(s)	183, 777
Asp700I	GAANNNNTTC	2 cut(s)	804, 1071
AspLEI	GCGC	3 cut(s)	545, 1193, 1289
AspS9I	GGNCC	4 cut(s)	181, 502, 653, 1227
AsuC2I	CCSGG	1 cut(s)	506
AvaI	CYCGRG	2 cut(s)	292, 674
AvaII	GGWCC	4 cut(s)	181, 502, 653, 1227
BalI	TGGCCA	1 cut(s)	1059
BamHI	GGATCC	1 cut(s)	886
BanI	GGYRCC	1 cut(s)	1286
BanII	GRGCYC	1 cut(s)	675
BauI	CACGAG	1 cut(s)	744
BbsI	GAAGAC	2 cut(s)	425, 1285
Bbv12I	GWGCWC	2 cut(s)	675, 870
BbvI	GCAGC	2 cut(s)	402, 701
BccI	CCATC	2 cut(s)	212, 664
BceAI	ACGGC	4 cut(s)	4, 288, 888, 1274
BciT130I	CCWGG	2 cut(s)	1061, 1163
BciVI	GTATCC	1 cut(s)	1269
BcnI	CCSGG	1 cut(s)	506
BcoDI	GTCTC	4 cut(s)	112, 139, 142, 145
BcuI	ACTAGT	1 cut(s)	1279
BfaI	CTAG	3 cut(s)	222, 530, 1280
BfmI	CTRYAG	1 cut(s)	1104
BfoI	RGCGCY	2 cut(s)	1194, 1290
BfuAI	ACCTGC	1 cut(s)	1193
BfuI	GTATCC	1 cut(s)	1269
BisI	GCNGC	3 cut(s)	276, 416, 690
BlpI	GCTNAGC	1 cut(s)	63
BlsI	GCNGC	3 cut(s)	277, 417, 691
Bme1390I	CCNGG	3 cut(s)	506, 1061, 1163
Bme18I	GGWCC	4 cut(s)	181, 502, 653, 1227
BmeT110I	CYCGRG	2 cut(s)	292, 674
BmgT120I	GGNCC	4 cut(s)	181, 502, 653, 1227
BmiI	GGNNCC	2 cut(s)	888, 1288
BmrFI	CCNGG	3 cut(s)	506, 1061, 1163
BmsI	GCATC	2 cut(s)	477, 1005
BpiI	GAAGAC	2 cut(s)	425, 1285
BpmI	CTGGAG	2 cut(s)	14, 1145
Bpu1102I	GCTNAGC	1 cut(s)	63
BpuEI	CTTGAG	4 cut(s)	685, 920, 1154, 1243
BpuMI	CCSGG	1 cut(s)	506
BsaHI	GRCGYC	2 cut(s)	409, 1287
BsaI	GGTCTC	2 cut(s)	142, 145
BsaJI	CCNNGG	4 cut(s)	14, 93, 174, 825
BsaWI	WCCGGW	3 cut(s)	183, 241, 777
BsaXI	ACNNNNNCTCC	4 cut(s)	170, 200, 913, 943
Bse118I	RCCGGY	3 cut(s)	183, 346, 777
Bse1I	ACTGG	3 cut(s)	119, 318, 801
Bse3DI	GCAATG	2 cut(s)	558, 692
BseBI	CCWGG	2 cut(s)	1061, 1163
BseDI	CCNNGG	4 cut(s)	14, 93, 174, 825
BseMI	GCAATG	2 cut(s)	558, 692
BseMII	CTCAG	3 cut(s)	77, 775, 1336
BseNI	ACTGG	3 cut(s)	119, 318, 801
BseRI	GAGGAG	3 cut(s)	37, 191, 1013
BseXI	GCAGC	2 cut(s)	402, 701
Bsh1236I	CGCG	5 cut(s)	85, 145, 342, 442, 543
BshFI	GGCC	5 cut(s)	19, 92, 98, 170, 1059
BshNI	GGYRCC	1 cut(s)	1286
BshTI	ACCGGT	2 cut(s)	183, 777
BsiHKAI	GWGCWC	2 cut(s)	675, 870
BsiHKCI	CYCGRG	2 cut(s)	292, 674
BsiSI	CCGG	5 cut(s)	184, 242, 347, 505, 778
BslFI	GGGAC	2 cut(s)	394, 1183
BsmAI	GTCTC	4 cut(s)	112, 139, 142, 145
BsmBI	CGTCTC	2 cut(s)	112, 139
BsmFI	GGGAC	2 cut(s)	394, 1183
BsnI	GGCC	5 cut(s)	19, 92, 98, 170, 1059
Bso31I	GGTCTC	2 cut(s)	142, 145
BsoBI	CYCGRG	2 cut(s)	292, 674
Bsp1286I	GDGCHC	2 cut(s)	675, 870
Bsp1407I	TGTACA	1 cut(s)	929
Bsp143I	GATC	4 cut(s)	164, 460, 874, 886
Bsp1720I	GCTNAGC	1 cut(s)	63
Bsp19I	CCATGG	1 cut(s)	14
Bsp68I	TCGCGA	2 cut(s)	85, 342
BspACI	CCGC	4 cut(s)	60, 145, 275, 440
BspANI	GGCC	5 cut(s)	19, 92, 98, 170, 1059
BspCNI	CTCAG	3 cut(s)	76, 774, 1335
BspFNI	CGCG	5 cut(s)	85, 145, 342, 442, 543
BspLI	GGNNCC	2 cut(s)	888, 1288
BspMI	ACCTGC	1 cut(s)	1193
BspPI	GGATC	3 cut(s)	455, 881, 894
BspQI	GCTCTTC	2 cut(s)	74, 795
BspT107I	GGYRCC	1 cut(s)	1286
BspTNI	GGTCTC	2 cut(s)	142, 145
BsrBI	CCGCTC	1 cut(s)	62
BsrDI	GCAATG	2 cut(s)	558, 692
BsrFI	RCCGGY	3 cut(s)	183, 346, 777
BsrGI	TGTACA	1 cut(s)	929
BsrI	ACTGG	3 cut(s)	119, 318, 801
BssAI	RCCGGY	3 cut(s)	183, 346, 777
BssECI	CCNNGG	4 cut(s)	14, 93, 174, 825
BssMI	GATC	4 cut(s)	164, 460, 874, 886
BssNI	GRCGYC	2 cut(s)	409, 1287
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	2 cut(s)	14, 825
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	2 cut(s)	1061, 1163
Bst4CI	ACNGT	2 cut(s)	1041, 1341
Bst6I	CTCTTC	3 cut(s)	74, 795, 990
BstACI	GRCGYC	2 cut(s)	409, 1287
BstAUI	TGTACA	1 cut(s)	929
BstC8I	GCNNGC	3 cut(s)	344, 513, 818
BstDEI	CTNAG	3 cut(s)	63, 761, 1322
BstDSI	CCRYGG	1 cut(s)	14
BstFNI	CGCG	5 cut(s)	85, 145, 342, 442, 543
BstH2I	RGCGCY	2 cut(s)	1194, 1290
BstHHI	GCGC	3 cut(s)	545, 1193, 1289
BstKTI	GATC	4 cut(s)	167, 463, 877, 889
BstMAI	GTCTC	4 cut(s)	112, 139, 142, 145
BstMBI	GATC	4 cut(s)	164, 460, 874, 886
BstMWI	GCNNNNNNNGC	6 cut(s)	496, 540, 670, 909, 1190, 1199
BstNI	CCWGG	2 cut(s)	1061, 1163
BstNSI	RCATGY	2 cut(s)	515, 1247
BstSCI	CCNGG	3 cut(s)	504, 1059, 1161
BstSFI	CTRYAG	1 cut(s)	1104
BstUI	CGCG	5 cut(s)	85, 145, 342, 442, 543
BstV1I	GCAGC	2 cut(s)	402, 701
BstV2I	GAAGAC	2 cut(s)	425, 1285
BstX2I	RGATCY	1 cut(s)	886
BstYI	RGATCY	1 cut(s)	886
BsuI	GTATCC	1 cut(s)	1269
BsuRI	GGCC	5 cut(s)	19, 92, 98, 170, 1059
BtgI	CCRYGG	1 cut(s)	14
BtgZI	GCGATG	1 cut(s)	320
BtsIMutI	CAGTG	2 cut(s)	325, 769
BtuMI	TCGCGA	2 cut(s)	85, 342
BveI	ACCTGC	1 cut(s)	1193
Cac8I	GCNNGC	3 cut(s)	344, 513, 818
CaiI	CAGNNNCTG	1 cut(s)	418
CfoI	GCGC	3 cut(s)	545, 1193, 1289
Cfr10I	RCCGGY	3 cut(s)	183, 346, 777
Cfr13I	GGNCC	4 cut(s)	181, 502, 653, 1227
Csp6I	GTAC	2 cut(s)	22, 930
CspAI	ACCGGT	2 cut(s)	183, 777
CviAII	CATG	7 cut(s)	15, 512, 893, 955, 1055, 1216, 1244
CviQI	GTAC	2 cut(s)	22, 930
DdeI	CTNAG	3 cut(s)	63, 761, 1322
DinI	GGCGCC	1 cut(s)	1288
DpnI	GATC	4 cut(s)	166, 462, 876, 888
DpnII	GATC	4 cut(s)	164, 460, 874, 886
EaeI	YGGCCR	3 cut(s)	17, 168, 1057
Eam1104I	CTCTTC	3 cut(s)	74, 795, 990
EarI	CTCTTC	3 cut(s)	74, 795, 990
Ecl136II	GAGCTC	1 cut(s)	673
Eco130I	CCWWGG	2 cut(s)	14, 825
Eco147I	AGGCCT	1 cut(s)	98
Eco24I	GRGCYC	1 cut(s)	675
Eco31I	GGTCTC	2 cut(s)	142, 145
Eco32I	GATATC	1 cut(s)	387
Eco47I	GGWCC	4 cut(s)	181, 502, 653, 1227
Eco53kI	GAGCTC	1 cut(s)	673
Eco57I	CTGAAG	2 cut(s)	440, 1011
Eco88I	CYCGRG	2 cut(s)	292, 674
EcoICRI	GAGCTC	1 cut(s)	673
EcoO109I	RGGNCCY	1 cut(s)	1227
EcoRI	GAATTC	1 cut(s)	336
EcoRII	CCWGG	2 cut(s)	1059, 1161
EcoRV	GATATC	1 cut(s)	387
EcoT14I	CCWWGG	2 cut(s)	14, 825
EcoT22I	ATGCAT	1 cut(s)	914
EcoT38I	GRGCYC	1 cut(s)	675
EgeI	GGCGCC	1 cut(s)	1288
EheI	GGCGCC	1 cut(s)	1288
ErhI	CCWWGG	2 cut(s)	14, 825
Esp3I	CGTCTC	2 cut(s)	112, 139
FaeI	CATG	7 cut(s)	18, 515, 896, 958, 1058, 1219, 1247
FalI	AAGNNNNNCTT	4 cut(s)	586, 618, 809, 841
FaqI	GGGAC	2 cut(s)	394, 1183
FatI	CATG	7 cut(s)	14, 511, 892, 954, 1054, 1215, 1243
FblI	GTMKAC	2 cut(s)	351, 1311
Fnu4HI	GCNGC	3 cut(s)	276, 416, 690
FriOI	GRGCYC	1 cut(s)	675
Fsp4HI	GCNGC	3 cut(s)	276, 416, 690
FspBI	CTAG	3 cut(s)	222, 530, 1280
GlaI	GCGC	3 cut(s)	544, 1192, 1288
GluI	GCNGC	3 cut(s)	276, 416, 690
GsuI	CTGGAG	2 cut(s)	14, 1145
HaeII	RGCGCY	2 cut(s)	1194, 1290
HaeIII	GGCC	5 cut(s)	19, 92, 98, 170, 1059
HapII	CCGG	5 cut(s)	184, 242, 347, 505, 778
HhaI	GCGC	3 cut(s)	545, 1193, 1289
Hin1I	GRCGYC	2 cut(s)	409, 1287
Hin1II	CATG	7 cut(s)	18, 515, 896, 958, 1058, 1219, 1247
Hin6I	GCGC	3 cut(s)	543, 1191, 1287
HinP1I	GCGC	3 cut(s)	543, 1191, 1287
HincII	GTYRAC	2 cut(s)	247, 783
HindII	GTYRAC	2 cut(s)	247, 783
HinfI	GANTC	7 cut(s)	10, 115, 323, 526, 1046, 1079, 1100
HpaI	GTTAAC	1 cut(s)	783
HpaII	CCGG	5 cut(s)	184, 242, 347, 505, 778
Hpy166II	GTNNAC	6 cut(s)	247, 352, 783, 932, 1263, 1312
Hpy188I	TCNGA	7 cut(s)	9, 114, 211, 335, 453, 1128, 1298
Hpy188III	TCNNGA	6 cut(s)	84, 341, 702, 937, 1076, 1324
Hpy8I	GTNNAC	6 cut(s)	247, 352, 783, 932, 1263, 1312
Hpy99I	CGWCG	4 cut(s)	236, 271, 274, 283
HpyAV	CCTTC	3 cut(s)	364, 474, 501
HpyCH4III	ACNGT	2 cut(s)	1041, 1341
HpyCH4IV	ACGT	2 cut(s)	409, 1172
HpyCH4V	TGCA	4 cut(s)	551, 556, 912, 1202
HpyF10VI	GCNNNNNNNGC	6 cut(s)	496, 540, 670, 909, 1190, 1199
HpyF3I	CTNAG	3 cut(s)	63, 761, 1322
HpySE526I	ACGT	2 cut(s)	409, 1172
Hsp92I	GRCGYC	2 cut(s)	409, 1287
Hsp92II	CATG	7 cut(s)	18, 515, 896, 958, 1058, 1219, 1247
HspAI	GCGC	3 cut(s)	543, 1191, 1287
KasI	GGCGCC	1 cut(s)	1286
KspAI	GTTAAC	1 cut(s)	783
Kzo9I	GATC	4 cut(s)	164, 460, 874, 886
LguI	GCTCTTC	2 cut(s)	74, 795
LmnI	GCTCC	2 cut(s)	39, 508
Lsp1109I	GCAGC	2 cut(s)	402, 701
LweI	GCATC	2 cut(s)	477, 1005
MaeI	CTAG	3 cut(s)	222, 530, 1280
MaeII	ACGT	2 cut(s)	409, 1172
MaeIII	GTNAC	1 cut(s)	756
MalI	GATC	4 cut(s)	166, 462, 876, 888
MbiI	CCGCTC	1 cut(s)	62
MboI	GATC	4 cut(s)	164, 460, 874, 886
MfeI	CAATTG	1 cut(s)	546
MflI	RGATCY	1 cut(s)	886
MhlI	GDGCHC	2 cut(s)	675, 870
MlsI	TGGCCA	1 cut(s)	1059
MluCI	AATT	9 cut(s)	336, 481, 546, 833, 925, 1008, 1027, 1114, 1151
MluNI	TGGCCA	1 cut(s)	1059
Mly113I	GGCGCC	1 cut(s)	1287
MlyI	GAGTC	1 cut(s)	4
Mox20I	TGGCCA	1 cut(s)	1059
Mph1103I	ATGCAT	1 cut(s)	914
MroXI	GAANNNNTTC	2 cut(s)	804, 1071
MscI	TGGCCA	1 cut(s)	1059
MseI	TTAA	1 cut(s)	782
Msp20I	TGGCCA	1 cut(s)	1059
MspA1I	CMGCKG	1 cut(s)	415
MspI	CCGG	5 cut(s)	184, 242, 347, 505, 778
MspR9I	CCNGG	3 cut(s)	506, 1061, 1163
MunI	CAATTG	1 cut(s)	546
MvaI	CCWGG	2 cut(s)	1061, 1163
MvnI	CGCG	5 cut(s)	85, 145, 342, 442, 543
MwoI	GCNNNNNNNGC	6 cut(s)	496, 540, 670, 909, 1190, 1199
NarI	GGCGCC	1 cut(s)	1287
NciI	CCSGG	1 cut(s)	506
NcoI	CCATGG	1 cut(s)	14
NdeII	GATC	4 cut(s)	164, 460, 874, 886
NlaIII	CATG	7 cut(s)	18, 515, 896, 958, 1058, 1219, 1247
NlaIV	GGNNCC	2 cut(s)	888, 1288
NmeAIII	GCCGAG	1 cut(s)	118
NruI	TCGCGA	2 cut(s)	85, 342
NsiI	ATGCAT	1 cut(s)	914
NspI	RCATGY	2 cut(s)	515, 1247
PaeI	GCATGC	1 cut(s)	515
PaeR7I	CTCGAG	1 cut(s)	674
PceI	AGGCCT	1 cut(s)	98
PciI	ACATGT	1 cut(s)	1243
PciSI	GCTCTTC	2 cut(s)	74, 795
PcsI	WCGNNNNNNNCGW	4 cut(s)	275, 278, 339, 491
PdmI	GAANNNNTTC	2 cut(s)	804, 1071
PfeI	GAWTC	6 cut(s)	115, 323, 526, 1046, 1079, 1100
PfoI	TCCNGGA	1 cut(s)	504
PinAI	ACCGGT	2 cut(s)	183, 777
PkrI	GCNGC	3 cut(s)	277, 417, 691
PleI	GAGTC	1 cut(s)	4
PluTI	GGCGCC	1 cut(s)	1290
PpsI	GAGTC	1 cut(s)	4
PpuMI	RGGWCCY	1 cut(s)	1227
PscI	ACATGT	1 cut(s)	1243
Psp124BI	GAGCTC	1 cut(s)	675
Psp5II	RGGWCCY	1 cut(s)	1227
Psp6I	CCWGG	2 cut(s)	1059, 1161
PspGI	CCWGG	2 cut(s)	1059, 1161
PspN4I	GGNNCC	2 cut(s)	888, 1288
PspPI	GGNCC	4 cut(s)	181, 502, 653, 1227
PspPPI	RGGWCCY	1 cut(s)	1227
PspXI	VCTCGAGB	1 cut(s)	674
PstNI	CAGNNNCTG	1 cut(s)	418
PsuI	RGATCY	1 cut(s)	886
PvuII	CAGCTG	1 cut(s)	415
RruI	TCGCGA	2 cut(s)	85, 342
RsaI	GTAC	2 cut(s)	23, 931
RsaNI	GTAC	2 cut(s)	22, 930
SacI	GAGCTC	1 cut(s)	675
SapI	GCTCTTC	2 cut(s)	74, 795
SaqAI	TTAA	1 cut(s)	782
SatI	GCNGC	3 cut(s)	276, 416, 690
Sau3AI	GATC	4 cut(s)	164, 460, 874, 886
Sau96I	GGNCC	4 cut(s)	181, 502, 653, 1227
SchI	GAGTC	1 cut(s)	4
ScrFI	CCNGG	3 cut(s)	506, 1061, 1163
SduI	GDGCHC	2 cut(s)	675, 870
SfaNI	GCATC	2 cut(s)	477, 1005
SfcI	CTRYAG	1 cut(s)	1104
SfoI	GGCGCC	1 cut(s)	1288
Sfr274I	CTCGAG	1 cut(s)	674
SinI	GGWCC	4 cut(s)	181, 502, 653, 1227
SlaI	CTCGAG	1 cut(s)	674
SmlI	CTYRAG	5 cut(s)	674, 700, 935, 1133, 1222
SmoI	CTYRAG	5 cut(s)	674, 700, 935, 1133, 1222
SpeI	ACTAGT	1 cut(s)	1279
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	9 cut(s)	336, 481, 546, 833, 925, 1008, 1027, 1114, 1151
SseBI	AGGCCT	1 cut(s)	98
SsiI	CCGC	4 cut(s)	60, 145, 275, 440
SspDI	GGCGCC	1 cut(s)	1286
SspMI	CTAG	3 cut(s)	222, 530, 1280
SstI	GAGCTC	1 cut(s)	675
StuI	AGGCCT	1 cut(s)	98
StyD4I	CCNGG	3 cut(s)	504, 1059, 1161
StyI	CCWWGG	2 cut(s)	14, 825
TaaI	ACNGT	2 cut(s)	1041, 1341
TaiI	ACGT	2 cut(s)	412, 1175
TaqI	TCGA	6 cut(s)	485, 625, 675, 842, 1077, 1236
TaqII	GACCGA	1 cut(s)	127
TasI	AATT	9 cut(s)	336, 481, 546, 833, 925, 1008, 1027, 1114, 1151
TatI	WGTACW	1 cut(s)	929
TauI	GCSGC	1 cut(s)	278
TfiI	GAWTC	6 cut(s)	115, 323, 526, 1046, 1079, 1100
Tru1I	TTAA	1 cut(s)	782
Tru9I	TTAA	1 cut(s)	782
TscAI	CASTG	2 cut(s)	325, 769
TseI	GCWGC	2 cut(s)	415, 689
TspDTI	ATGAA	5 cut(s)	456, 595, 659, 909, 1232
TspGWI	ACGGA	1 cut(s)	121
TspRI	CASTG	2 cut(s)	325, 769
VpaK11BI	GGWCC	4 cut(s)	181, 502, 653, 1227
XapI	RAATTY	2 cut(s)	336, 481
XceI	RCATGY	2 cut(s)	515, 1247
XhoI	CTCGAG	1 cut(s)	674
XmiI	GTMKAC	2 cut(s)	351, 1311
XmnI	GAANNNNTTC	2 cut(s)	804, 1071
XspI	CTAG	3 cut(s)	222, 530, 1280
ZraI	GACGTC	1 cut(s)	410
Zsp2I	ATGCAT	1 cut(s)	914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.