MD03G1194600.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012393
Physical Location & Seq
Reverse (-)
26660451 .. 26661822
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1194600.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGCCACCTTCACATTCCCTCTCTTCCTCTCTCTCCTCCTCCCAATCTTCGCCTTTGCAGCTTCCGCTGAAACAGGTACTGTTGGAATAAACTACGGCAGGATAGCAGACAACTTACCACCGCCCGAAAAAGTGGTGGCACTCCTCAAATCCCAAGGCATCAACAAGGTGAAGCTCTATGACACCGACGCCACCGTCCTCACGGCCCTCGCTAACTCCGGCATTAGCGTCGTCGTCGCCCTCCCCAACGAGCTCCTCTCTTCCGCCGCAAGCGACCCCTCGTTCGCCGACAAATGGGTCCAATCCAACATCTCCCACTACCACCCCAAAACCCAAATCGAAGCCATCGCCGTCGGCAACGAGGTCTTTGTCGACCCAAACAACACCACCCAATTCCTCGTCCCTGCCATGAAAAACATCCAATCTTCCCTAGTCAAGTACAACCTCAGCTCCATCAAACTCTCTTCCCCAATCGCCCTCAGCGCCCTCAATTCCTCGTACCCGCCTTCCGCCGGGGCCTTCAACCCCGACTTAATCGAACCCGTCATCAAACCGTTTTTGGAATTTTTAACCCAGACGTCGTCGTATTTGATGATAAACGCCTACCCCTTTTTCGCGTACGAGTCCACCTCCGACAAGATTTCTCTCGACTATGCTCTATTCCGGACCAACCCGGGAAATCCAGACTCGGGCAACGGGCTGAAATACTACAGTTTGCTAGAGGCCCAAATCGACGCCGTTTTCGCCGCCATGTCGGCTGTGGGACACGGCGACGTAAAGGGCAAGCCAATGCGGCGGCGTATAACGGAAACTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

273

Amino Acids

29.42

Weight (kDa)

5.62

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 28 - 259 4e-57 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26830 AT4G26830 AT5G55180 AT5G55180
fragaria_vesca FvH4_3g23410 FvH4_3g23410
malus_domestica MD03G1194600.v1.1 MD11G1210800.v1.1
prunus_persica Prupe.4G222100_v2.0.a1
pyrus_communis pycom03g14670 pycom11g18630
rosa_chinensis RchiOBHm_Chr5g0041211
rosa_laevigata RLG00000034037
rosa_multiflora Rmu_sc0003502.1_g000013
rosa_roxburghii Rroxscaffold_1G00039410
rosa_rugosa Rorug05G0190600
rosa_samantha Rh5BG281600 Rh5CG313600
rosa_wichuraiana Rw5G026020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 194, 296
AatII GACGTC 1 cut(s) 581
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 617
AccIII TCCGGA 1 cut(s) 663
AciI CCGC 9 cut(s) 66, 122, 264, 267, 503, 510, 747, 793, 796
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 563
AcyI GRCGYC 3 cut(s) 189, 578, 735
AfaI GTAC 4 cut(s) 79, 440, 500, 620
AfiI CCNNNNNNNGG 3 cut(s) 294, 512, 689
AgsI TTSAA 1 cut(s) 523
AluBI AGCT 4 cut(s) 62, 175, 253, 450
AluI AGCT 4 cut(s) 62, 175, 253, 450
Alw21I GWGCWC 1 cut(s) 255
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 2 cut(s) 673, 688
Aor13HI TCCGGA 1 cut(s) 663
AoxI GGCC 4 cut(s) 3, 204, 516, 723
ApeKI GCWGC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 563
ArsI GACNNNNNNTTYG 4 cut(s) 266, 298, 725, 757
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 5 cut(s) 205, 298, 516, 666, 724
AsuC2I CCSGG 3 cut(s) 514, 674, 675
AsuHPI GGTGA 1 cut(s) 181
AvaI CYCGRG 2 cut(s) 673, 688
AvaII GGWCC 2 cut(s) 298, 666
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 2 cut(s) 446, 479
BbvI GCAGC 1 cut(s) 71
BccI CCATC 2 cut(s) 353, 461
BceAI ACGGC 5 cut(s) 112, 219, 335, 722, 784
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BcnI CCSGG 3 cut(s) 514, 674, 675
BfaI CTAG 2 cut(s) 431, 719
BfmI CTRYAG 1 cut(s) 709
BfoI RGCGCY 1 cut(s) 486
BglI GCCNNNNNGGC 2 cut(s) 755, 793
BisI GCNGC 5 cut(s) 60, 267, 747, 794, 797
BlsI GCNGC 5 cut(s) 61, 268, 748, 795, 798
Bme1390I CCNGG 3 cut(s) 514, 674, 675
Bme18I GGWCC 2 cut(s) 298, 666
BmeT110I CYCGRG 2 cut(s) 673, 688
BmgT120I GGNCC 5 cut(s) 205, 298, 516, 666, 724
BmiI GGNNCC 2 cut(s) 299, 517
BmrFI CCNGG 3 cut(s) 514, 674, 675
BmsI GCATC 1 cut(s) 168
BplI GAGNNNNNCTC 4 cut(s) 242, 274, 614, 646
Bpu10I CCTNAGC 2 cut(s) 446, 479
BpuMI CCSGG 3 cut(s) 514, 674, 675
BsaHI GRCGYC 3 cut(s) 189, 578, 735
BsaJI CCNNGG 3 cut(s) 154, 513, 673
BsaWI WCCGGW 1 cut(s) 663
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 294, 512, 689
BseAI TCCGGA 1 cut(s) 663
BseDI CCNNGG 3 cut(s) 154, 513, 673
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 3 cut(s) 294, 512, 689
BseMII CTCAG 2 cut(s) 460, 493
BseRI GAGGAG 4 cut(s) 26, 29, 134, 245
BseXI GCAGC 1 cut(s) 71
Bsh1236I CGCG 1 cut(s) 617
BshFI GGCC 4 cut(s) 5, 206, 518, 725
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 2 cut(s) 673, 688
BsiSI CCGG 4 cut(s) 219, 513, 664, 674
BsiWI CGTACG 1 cut(s) 618
BslFI GGGAC 2 cut(s) 386, 777
BslI CCNNNNNNNGG 3 cut(s) 294, 512, 689
BsmFI GGGAC 2 cut(s) 386, 777
BsnI GGCC 4 cut(s) 5, 206, 518, 725
BsoBI CYCGRG 2 cut(s) 673, 688
Bsp1286I GDGCHC 1 cut(s) 255
Bsp13I TCCGGA 1 cut(s) 663
BspACI CCGC 9 cut(s) 66, 122, 264, 267, 503, 510, 747, 793, 796
BspANI GGCC 4 cut(s) 5, 206, 518, 725
BspCNI CTCAG 2 cut(s) 459, 492
BspEI TCCGGA 1 cut(s) 663
BspFNI CGCG 1 cut(s) 617
BspLI GGNNCC 2 cut(s) 299, 517
BssECI CCNNGG 3 cut(s) 154, 513, 673
BssNI GRCGYC 3 cut(s) 189, 578, 735
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 4 cut(s) 82, 196, 555, 713
Bst6I CTCTTC 3 cut(s) 29, 265, 469
BstACI GRCGYC 3 cut(s) 189, 578, 735
BstC8I GCNNGC 2 cut(s) 271, 785
BstDEI CTNAG 2 cut(s) 446, 479
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 1 cut(s) 617
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 1 cut(s) 485
BstMWI GCNNNNNNNGC 6 cut(s) 59, 65, 482, 743, 755, 793
BstSCI CCNGG 3 cut(s) 512, 672, 673
BstSFI CTRYAG 1 cut(s) 709
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 1 cut(s) 71
BsuRI GGCC 4 cut(s) 5, 206, 518, 725
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 271, 785
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 5 cut(s) 205, 298, 516, 666, 724
Cfr9I CCCGGG 1 cut(s) 673
CseI GACGC 3 cut(s) 197, 217, 743
Csp6I GTAC 4 cut(s) 78, 439, 499, 619
CviAII CATG 2 cut(s) 409, 751
CviQI GTAC 4 cut(s) 78, 439, 499, 619
DdeI CTNAG 2 cut(s) 446, 479
DrdI GACNNNNNNGTC 2 cut(s) 194, 296
DseDI GACNNNNNNGTC 2 cut(s) 194, 296
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 29, 265, 469
EarI CTCTTC 3 cut(s) 29, 265, 469
EciI GGCGGA 2 cut(s) 253, 499
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 298, 666
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 2 cut(s) 673, 688
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 412, 754
FaiI YATR 5 cut(s) 180, 410, 654, 752, 803
FaqI GGGAC 2 cut(s) 386, 777
FatI CATG 2 cut(s) 408, 750
FauI CCCGC 1 cut(s) 510
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 5 cut(s) 60, 267, 747, 794, 797
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 5 cut(s) 60, 267, 747, 794, 797
FspBI CTAG 2 cut(s) 431, 719
GlaI GCGC 1 cut(s) 484
GluI GCNGC 5 cut(s) 60, 267, 747, 794, 797
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 4 cut(s) 5, 206, 518, 725
HapII CCGG 4 cut(s) 219, 513, 664, 674
HgaI GACGC 3 cut(s) 197, 217, 743
HhaI GCGC 1 cut(s) 485
Hin1I GRCGYC 3 cut(s) 189, 578, 735
Hin1II CATG 2 cut(s) 412, 754
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 2 cut(s) 623, 686
HpaII CCGG 4 cut(s) 219, 513, 664, 674
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 373, 627
Hpy188I TCNGA 1 cut(s) 634
Hpy188III TCNNGA 3 cut(s) 647, 664, 683
Hpy8I GTNNAC 2 cut(s) 373, 627
Hpy99I CGWCG 9 cut(s) 191, 233, 236, 239, 356, 583, 586, 737, 776
HpyAV CCTTC 3 cut(s) 19, 516, 529
HpyCH4III ACNGT 4 cut(s) 82, 196, 555, 713
HpyCH4IV ACGT 2 cut(s) 578, 774
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 65, 482, 743, 755, 793
HpyF3I CTNAG 2 cut(s) 446, 479
HpySE526I ACGT 2 cut(s) 578, 774
Hsp92I GRCGYC 3 cut(s) 189, 578, 735
Hsp92II CATG 2 cut(s) 412, 754
HspAI GCGC 1 cut(s) 483
Kpn2I TCCGGA 1 cut(s) 663
LmnI GCTCC 2 cut(s) 258, 455
LpnPI CCDG 9 cut(s) 60, 85, 232, 417, 526, 587, 677, 687, 696
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 431, 719
MaeII ACGT 2 cut(s) 578, 774
MboII GAAGA 5 cut(s) 16, 40, 252, 417, 456
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 392, 490, 563
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 632, 680
MmeI TCCRAC 3 cut(s) 64, 330, 657
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 663
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 533, 569
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 4 cut(s) 219, 513, 664, 674
MspR9I CCNGG 3 cut(s) 514, 674, 675
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 6 cut(s) 59, 65, 482, 743, 755, 793
NciI CCSGG 3 cut(s) 514, 674, 675
NlaIII CATG 2 cut(s) 412, 754
NlaIV GGNNCC 2 cut(s) 299, 517
Pfl23II CGTACG 1 cut(s) 618
PflFI GACNNNGTC 1 cut(s) 580
PkrI GCNGC 5 cut(s) 61, 268, 748, 795, 798
PleI GAGTC 2 cut(s) 631, 680
PpsI GAGTC 2 cut(s) 631, 680
Psp124BI GAGCTC 1 cut(s) 255
PspLI CGTACG 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 299, 517
PspPI GGNCC 5 cut(s) 205, 298, 516, 666, 724
PstNI CAGNNNCTG 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 580
RsaI GTAC 4 cut(s) 79, 440, 500, 620
RsaNI GTAC 4 cut(s) 78, 439, 499, 619
SacI GAGCTC 1 cut(s) 255
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 533, 569
SatI GCNGC 5 cut(s) 60, 267, 747, 794, 797
Sau96I GGNCC 5 cut(s) 205, 298, 516, 666, 724
SchI GAGTC 2 cut(s) 632, 680
ScrFI CCNGG 3 cut(s) 514, 674, 675
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 2 cut(s) 298, 666
SmaI CCCGGG 1 cut(s) 675
Sse9I AATT 3 cut(s) 392, 490, 563
SsiI CCGC 9 cut(s) 66, 122, 264, 267, 503, 510, 747, 793, 796
SspMI CTAG 2 cut(s) 431, 719
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 512, 672, 673
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 4 cut(s) 82, 196, 555, 713
TaiI ACGT 2 cut(s) 581, 777
TaqI TCGA 5 cut(s) 339, 372, 537, 648, 732
TasI AATT 3 cut(s) 392, 490, 563
TatI WGTACW 1 cut(s) 438
TauI GCSGC 4 cut(s) 269, 749, 796, 799
Tru1I TTAA 2 cut(s) 533, 569
Tru9I TTAA 2 cut(s) 533, 569
TseI GCWGC 1 cut(s) 59
TspDTI ATGAA 1 cut(s) 425
TspMI CCCGGG 1 cut(s) 673
Tth111I GACNNNGTC 1 cut(s) 580
VpaK11BI GGWCC 2 cut(s) 298, 666
XapI RAATTY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XmaI CCCGGG 1 cut(s) 673
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 2 cut(s) 431, 719
ZraI GACGTC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.