MD03G1264300.v1.1

cyclin-dependent protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014458
Physical Location & Seq
Forward (+)
34995004 .. 34997135
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1264300.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGTCTGCGCCTTTGCACAGAATCATCACACACCTGCCATGAACACTCTCACTGCCAGAGGCAGTACGTATTCAAGAGAAATCGTACCAATTTACACAGCAAACAGTTTTCTTGAGATGAACCAAGAGATCTACCCACCACATGTTAAGGTTCTAATGCTACTCTTATCCCTTTTTTCAGTAAGCACACGGATATCTTCAATTGGACCAGCGCATGGATTCCTTCATCGGAGCAGCCAAGCTCCATCCTCACCAATCAGACCCACCTCCCCATTTCTCGGCAGAAAACTTAAAATCAAACCCACATTCGAATCCCAGCAACCTAGTTCATCAAGAATCGCAATTTCAAACTTCAAAACGCTGCTTGTGTTTGCGTCCAGCAGCGGAGAGTTGATCCACGCCGTCCATGACATGTTTATGGGTGTGGGATTGGGGCTGCCGTGCACGGTGATGGAGTGCGACGACATCATGTACAGAAGCACTAGTCCCCAAATCCGATGGCTTGACGCTCACGACCCCCGGTGCTGTTCTGGTTCTGGGTGCCCTGTCGTATTTCTGGGCCACCCTCGGCGTGGCTCCATGGCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

197

Amino Acids

21.52

Weight (kDa)

9.21

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68830
fragaria_vesca FvH4_4g28080
malus_domestica MD03G1264300.v1.1 MD13G1046400.v1.1
prunus_persica Prupe.1G305500_v2.0.a1
pyrus_communis pycom13g04070
rosa_chinensis RchiOBHm_Chr4g0435821
rosa_laevigata RLG00000006556
rosa_multiflora Rmu_sc0002642.1_g000004 Rmu_sc0010838.1_g000002
rosa_roxburghii Rroxscaffold_5G00377000
rosa_rugosa Rorug04G0289800
rosa_samantha Rh4AG344100 Rh4BG353000 Rh4CG367400 Rh4DG346900
rosa_wichuraiana Rw4G030060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 215
AciI CCGC 1 cut(s) 384
AclWI GGATC 1 cut(s) 388
AfaI GTAC 3 cut(s) 67, 87, 473
AfiI CCNNNNNNNGG 3 cut(s) 215, 278, 572
AflIII ACRYGT 2 cut(s) 142, 411
AgsI TTSAA 4 cut(s) 75, 201, 348, 355
AhlI ACTAGT 1 cut(s) 482
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw21I GWGCWC 1 cut(s) 446
Alw44I GTGCAC 1 cut(s) 442
AlwI GGATC 1 cut(s) 388
AoxI GGCC 1 cut(s) 559
ApaLI GTGCAC 1 cut(s) 442
ApeKI GCWGC 4 cut(s) 234, 361, 381, 436
AspLEI GCGC 2 cut(s) 11, 214
AspS9I GGNCC 2 cut(s) 206, 559
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 2 cut(s) 243, 460
AsuII TTCGAA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 2 cut(s) 446, 545
BanI GGYRCC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 4 cut(s) 246, 348, 393, 423
BccI CCATC 3 cut(s) 253, 445, 492
BceAI ACGGC 2 cut(s) 386, 424
BcnI CCSGG 1 cut(s) 520
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 324, 483
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 129
BisI GCNGC 4 cut(s) 235, 362, 382, 437
BlsI GCNGC 4 cut(s) 236, 363, 383, 438
Bme1390I CCNGG 1 cut(s) 520
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 206, 559
BmiI GGNNCC 2 cut(s) 542, 577
BmrFI CCNGG 1 cut(s) 520
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 520
BsaAI YACGTR 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 518, 566, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 278, 572
BseDI CCNNGG 3 cut(s) 518, 566, 579
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 3 cut(s) 215, 278, 572
BseSI GKGCMC 2 cut(s) 446, 545
BseXI GCAGC 4 cut(s) 246, 348, 393, 423
BseYI CCCAGC 1 cut(s) 315
BshFI GGCC 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 446
BsiSI CCGG 1 cut(s) 520
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 3 cut(s) 215, 278, 572
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 1 cut(s) 561
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 446, 545
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 2 cut(s) 129, 393
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 1 cut(s) 384
BspANI GGCC 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 542, 577
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 1 cut(s) 388
BspT104I TTCGAA 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 540
BsrGI TGTACA 1 cut(s) 471
BssECI CCNNGG 3 cut(s) 518, 566, 579
BssMI GATC 2 cut(s) 129, 393
BssT1I CCWWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 107, 448
BstAUI TGTACA 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 1 cut(s) 245
BstHHI GCGC 2 cut(s) 11, 214
BstKTI GATC 2 cut(s) 132, 396
BstMBI GATC 2 cut(s) 129, 393
BstNSI RCATGY 2 cut(s) 146, 415
BstSCI CCNGG 1 cut(s) 518
BstSLI GKGCMC 2 cut(s) 446, 545
BstSNI TACGTA 1 cut(s) 69
BstV1I GCAGC 4 cut(s) 246, 348, 393, 423
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BsuRI GGCC 1 cut(s) 561
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 1 cut(s) 245
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 43
CfoI GCGC 2 cut(s) 11, 214
Cfr13I GGNCC 2 cut(s) 206, 559
CseI GACGC 2 cut(s) 363, 515
Csp6I GTAC 3 cut(s) 66, 86, 472
CviAII CATG 7 cut(s) 40, 143, 215, 407, 412, 469, 580
CviJI RGCY 7 cut(s) 237, 242, 436, 502, 561, 576, 584
CviKI_1 RGCY 7 cut(s) 237, 242, 436, 502, 561, 576, 584
CviQI GTAC 3 cut(s) 66, 86, 472
DpnI GATC 2 cut(s) 131, 395
DpnII GATC 2 cut(s) 129, 393
Eco105I TACGTA 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 579
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 206
EcoRV GATATC 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 7 cut(s) 43, 146, 218, 410, 415, 472, 583
FaiI YATR 8 cut(s) 41, 144, 216, 408, 413, 419, 470, 581
FaqI GGGAC 1 cut(s) 471
FatI CATG 7 cut(s) 39, 142, 214, 406, 411, 468, 579
Fnu4HI GCNGC 4 cut(s) 235, 362, 382, 437
FokI GGATG 1 cut(s) 232
Fsp4HI GCNGC 4 cut(s) 235, 362, 382, 437
FspBI CTAG 2 cut(s) 324, 483
GlaI GCGC 2 cut(s) 10, 213
GluI GCNGC 4 cut(s) 235, 362, 382, 437
GsaI CCCAGC 1 cut(s) 319
HaeIII GGCC 1 cut(s) 561
HapII CCGG 1 cut(s) 520
HgaI GACGC 2 cut(s) 363, 515
HhaI GCGC 2 cut(s) 11, 214
Hin1II CATG 7 cut(s) 43, 146, 218, 410, 415, 472, 583
Hin6I GCGC 2 cut(s) 9, 212
HinP1I GCGC 2 cut(s) 9, 212
HinfI GANTC 4 cut(s) 22, 219, 311, 336
HpaII CCGG 1 cut(s) 520
HphI GGTGA 2 cut(s) 243, 460
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 3 cut(s) 231, 260, 497
Hpy188III TCNNGA 4 cut(s) 75, 113, 333, 512
Hpy8I GTNNAC 1 cut(s) 444
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 107, 448
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 2 cut(s) 17, 444
HpySE526I ACGT 1 cut(s) 68
Hsp92II CATG 7 cut(s) 43, 146, 218, 410, 415, 472, 583
HspAI GCGC 2 cut(s) 9, 212
Kzo9I GATC 2 cut(s) 129, 393
LmnI GCTCC 3 cut(s) 231, 247, 581
Lsp1109I GCAGC 4 cut(s) 246, 348, 393, 423
MaeI CTAG 2 cut(s) 324, 483
MaeII ACGT 1 cut(s) 68
MalI GATC 2 cut(s) 131, 395
MboI GATC 2 cut(s) 129, 393
MboII GAAGA 1 cut(s) 189
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 2 cut(s) 446, 545
MluCI AATT 3 cut(s) 90, 201, 342
MnlI CCTC 4 cut(s) 53, 259, 277, 576
MseI TTAA 2 cut(s) 147, 291
MslI CAYNNNNRTG 2 cut(s) 416, 449
MspA1I CMGCKG 1 cut(s) 384
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 1 cut(s) 520
MunI CAATTG 1 cut(s) 201
NciI CCSGG 1 cut(s) 520
NcoI CCATGG 1 cut(s) 579
NdeII GATC 2 cut(s) 129, 393
NlaIII CATG 7 cut(s) 43, 146, 218, 410, 415, 472, 583
NlaIV GGNNCC 2 cut(s) 542, 577
NmeAIII GCCGAG 2 cut(s) 258, 547
NspI RCATGY 2 cut(s) 146, 415
NspV TTCGAA 1 cut(s) 309
PaqCI CACCTGC 1 cut(s) 43
PciI ACATGT 2 cut(s) 142, 411
PfeI GAWTC 4 cut(s) 22, 219, 311, 336
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 4 cut(s) 236, 363, 383, 438
Ppu21I YACGTR 1 cut(s) 69
PscI ACATGT 2 cut(s) 142, 411
PspFI CCCAGC 1 cut(s) 315
PspN4I GGNNCC 2 cut(s) 542, 577
PspPI GGNCC 2 cut(s) 206, 559
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 3 cut(s) 67, 87, 473
RsaNI GTAC 3 cut(s) 66, 86, 472
RseI CAYNNNNRTG 2 cut(s) 416, 449
SaqAI TTAA 2 cut(s) 147, 291
SatI GCNGC 4 cut(s) 235, 362, 382, 437
Sau3AI GATC 2 cut(s) 129, 393
Sau96I GGNCC 2 cut(s) 206, 559
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 2 cut(s) 446, 545
SetI ASST 6 cut(s) 37, 71, 153, 244, 269, 325
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 416, 449
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SnaBI TACGTA 1 cut(s) 69
SpeI ACTAGT 1 cut(s) 482
Sse9I AATT 3 cut(s) 90, 201, 342
SsiI CCGC 1 cut(s) 384
SspMI CTAG 2 cut(s) 324, 483
StyD4I CCNGG 1 cut(s) 518
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 2 cut(s) 107, 448
TaiI ACGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 309
TasI AATT 3 cut(s) 90, 201, 342
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 4 cut(s) 22, 219, 311, 336
Tru1I TTAA 2 cut(s) 147, 291
Tru9I TTAA 2 cut(s) 147, 291
TscAI CASTG 1 cut(s) 58
TseI GCWGC 4 cut(s) 234, 361, 381, 436
TspDTI ATGAA 4 cut(s) 56, 134, 215, 318
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 206
XceI RCATGY 2 cut(s) 146, 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XspI CTAG 2 cut(s) 324, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.