Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014458

Physical Location & Seq

Genomic Coordinates Forward (+)

3215445 .. 3218931

Gene Structure 3487 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1046400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGGCGGCCACTGTCGCAGCCGGCGGAGTTAGCATCGGGACGGCCAAGCTCCGTCCTCACCAGTCAAAACCCACCTCCCCGTTTCTCGGCAAAAAACTCAAAATCAGACCTACCTTGGAATCCCAGCAACGCAGTTCGAGAATCACAATCTTAAACCCCAAAACGCTGCTGGTTTTCGCTTCCGGCGGCGGTGAGTTGATCCACGCCGTCAACGACCTGTTTCTGGGGGTGGGGGTGGGGCTGCCGTGCACGGTGATGGAGTGCGGCGACATCATATACAGAAGCACCCTTCCCAAATCCGATGGCTTGACGCTCACGGCCCCCGGTGCTGTTCTGGCTCTGGGCGCCCTGTCGTATCTCTGGGCCACCCCCGGCGTGGCTCCGGGGTTCTTTGACATGTTTGTGCTTGCTTTTATTGAGAGGCTCTTCAGACCCACTTACAAAAAGGATGATTTTGTTTTGGGGAAAAAGTTGGGAGAAGGTGCTTTTGGAGTTGTTTACAAAGTTTCATTGGCTAACAAGAAGTCTTCTTCCAAGAAAGAAGGCGACTTGGTGTTGAAGAAGGCTACTGAATATGGCGCGGTAGAGATTTGGATGAACGAGCGTGTGCGGAGGGCGTGTGCGAACAGCTGTGCTGATTTTTTGTACGGCTTTCTTGAGAGTTCTTCTGCAAAGAAAGGACCTGAATATTGGCTCATATGGCGGTTTGAAGGGGCGGCCACTCTTTATGATTTGATGCAAAGTAAAGAGTTTCCGTACAATGTAGAAACAATGATTCTTGGAAAGGTTCAGGACTTGCCGAAGGGTTTGGAAAGGGAAAACAGAATCATACAAACGATAATGAGACAGCTCTTATTCGCACTGGATGGTCTTCATTCAACTGGGATTGTGCATAGAGACATAAAGCCACAGAATGTTATTTTCTCTGAAGGCTCTCGTATATTCAAGATCATTGATCTTGGAGCTGCAGCAGACTTGCGAGTCGGCATCAACTACATCCCAAAGGAGTTTCTTTTGGATCCAAGATATGCTGCACCAGAGCAATACATCATGAGCACACAAACGCCATCTGCACCTTCTGCTCCTGTGGCAACAGCACTTTCCCCAGTTCTCTGGCAGATGAATTTACCGGATAGATTTGATATCTACAGTGCCGGTCTCATATTCCTACAAATGGCCTTCCCATCATTACGCAGTGACAACAGCCTAATACAATTCAACCGTCAGCTAAAAAGGTGCGATTATGACTTGGTTGCATGGAGAAAAGCCGCCGAGCCCCGAGCTAGCGCTGATCTACGAAGAGGTTTTGAGTTGTTGGATTTGGATGGTGGCATTGGATGGGAGCTCCTGACATCAATGGTTCGGTACAAAGCGAGACAGAGAATCAGTGCGAAAACAGCCTTAGCGTATCCCTACTTTGACAAGGAAGGACTGTTGGCTCTGTCCTTGATGCAGAACCTGAGGTTGCAAGTGTTTCGATATACACAACAAGATTATGGGGAGGCTGCCAAATGGATTATTCGGCTAATGGCACGATCAGGAACCGCGCAGGATGGTGGCTTCACAGAAGCTGAGCTCCAGGACCTTAGAGTTTGTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009643 GO:0009987 GO:0010109 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0031323 GO:0034357 GO:0036211 GO:0042548 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

534

Amino Acids

59.22

Weight (kDa)

9.27

Isoelectric Point (pI)

42.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	154 - 348	1.6e-08	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	242 - 348	4.3e-16	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014458)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68830
fragaria_vesca	FvH4_4g28080
malus_domestica	MD03G1264300.v1.1 MD13G1046400.v1.1
prunus_persica	Prupe.1G305500_v2.0.a1
pyrus_communis	pycom13g04070
rosa_chinensis	RchiOBHm_Chr4g0435821
rosa_laevigata	RLG00000006556
rosa_multiflora	Rmu_sc0002642.1_g000004 Rmu_sc0010838.1_g000002
rosa_roxburghii	Rroxscaffold_5G00377000
rosa_rugosa	Rorug04G0289800
rosa_samantha	Rh4AG344100 Rh4BG353000 Rh4CG367400 Rh4DG346900
rosa_wichuraiana	Rw4G030060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	980
AccB1I	GGYRCC	1 cut(s)	344
AccII	CGCG	2 cut(s)	581, 1547
AclWI	GGATC	3 cut(s)	193, 1013, 1026
AcoI	YGGCCR	3 cut(s)	6, 42, 717
AcsI	RAATTY	1 cut(s)	1123
AcuI	CTGAAG	2 cut(s)	412, 948
AcyI	GRCGYC	1 cut(s)	345
AfaI	GTAC	4 cut(s)	647, 758, 1367, 1597
AfeI	AGCGCT	1 cut(s)	1288
AfiI	CCNNNNNNNGG	4 cut(s)	86, 223, 376, 1174
AflIII	ACRYGT	1 cut(s)	396
AgsI	TTSAA	5 cut(s)	559, 710, 879, 946, 1219
AjnI	CCWGG	1 cut(s)	1578
AjuI	GAANNNNNNNTTGG	2 cut(s)	471, 503
AluBI	AGCT	9 cut(s)	49, 630, 850, 965, 1228, 1283, 1345, 1571, 1576
AluI	AGCT	9 cut(s)	49, 630, 850, 965, 1228, 1283, 1345, 1571, 1576
Alw21I	GWGCWC	4 cut(s)	251, 1058, 1347, 1578
Alw26I	GTCTC	4 cut(s)	838, 891, 1163, 1369
Alw44I	GTGCAC	1 cut(s)	247
AlwI	GGATC	3 cut(s)	193, 1013, 1026
AlwNI	CAGNNNCTG	2 cut(s)	1459, 1571
Ama87I	CYCGRG	1 cut(s)	1278
Aor51HI	AGCGCT	1 cut(s)	1288
AoxI	GGCC	6 cut(s)	6, 42, 318, 363, 717, 1176
ApaLI	GTGCAC	1 cut(s)	247
ApeKI	GCWGC	7 cut(s)	17, 166, 241, 965, 968, 1031, 1505
ApoI	RAATTY	1 cut(s)	1123
AspLEI	GCGC	4 cut(s)	347, 581, 1289, 1549
AspS9I	GGNCC	4 cut(s)	319, 363, 680, 1582
AsuC2I	CCSGG	3 cut(s)	324, 372, 384
AsuHPI	GGTGA	3 cut(s)	50, 203, 265
AsuNHI	GCTAGC	1 cut(s)	1283
AvaI	CYCGRG	1 cut(s)	1278
AvaII	GGWCC	2 cut(s)	680, 1582
AxyI	CCTNAGG	1 cut(s)	1460
BaeGI	GKGCMC	1 cut(s)	251
BamHI	GGATCC	1 cut(s)	1018
BanI	GGYRCC	1 cut(s)	344
BanII	GRGCYC	3 cut(s)	1278, 1347, 1578
BbsI	GAAGAC	2 cut(s)	519, 863
Bbv12I	GWGCWC	4 cut(s)	251, 1058, 1347, 1578
BbvI	GCAGC	7 cut(s)	29, 153, 228, 952, 980, 1018, 1492
BccI	CCATC	8 cut(s)	250, 296, 860, 1075, 1192, 1319, 1332, 1547
BceAI	ACGGC	5 cut(s)	57, 191, 229, 333, 664
BcgI	CGANNNNNNTGC	2 cut(s)	1457, 1491
BciT130I	CCWGG	1 cut(s)	1580
BciVI	GTATCC	1 cut(s)	1419
BcnI	CCSGG	3 cut(s)	324, 372, 384
BcoDI	GTCTC	4 cut(s)	838, 891, 1163, 1369
BfaI	CTAG	1 cut(s)	1284
BfmI	CTRYAG	2 cut(s)	966, 1147
BfoI	RGCGCY	2 cut(s)	348, 1290
BfuI	GTATCC	1 cut(s)	1419
BlpI	GCTNAGC	1 cut(s)	1572
Bme1390I	CCNGG	4 cut(s)	324, 372, 384, 1580
Bme18I	GGWCC	2 cut(s)	680, 1582
BmeT110I	CYCGRG	1 cut(s)	1278
BmgT120I	GGNCC	4 cut(s)	319, 363, 680, 1582
BmiI	GGNNCC	5 cut(s)	321, 346, 381, 1020, 1543
BmrFI	CCNGG	4 cut(s)	324, 372, 384, 1580
BmrI	ACTGGG	2 cut(s)	891, 1100
BmsI	GCATC	4 cut(s)	42, 726, 996, 1440
BmtI	GCTAGC	1 cut(s)	1287
BmuI	ACTGGG	2 cut(s)	891, 1100
BpiI	GAAGAC	2 cut(s)	519, 863
BpmI	CTGGAG	1 cut(s)	1562
Bpu10I	CCTNAGC	1 cut(s)	1402
Bpu1102I	GCTNAGC	1 cut(s)	1572
BpuEI	CTTGAG	1 cut(s)	677
BpuMI	CCSGG	3 cut(s)	324, 372, 384
BsaHI	GRCGYC	1 cut(s)	345
BsaI	GGTCTC	1 cut(s)	1163
BsaJI	CCNNGG	4 cut(s)	114, 322, 370, 383
BsaWI	WCCGGW	1 cut(s)	1129
BsaXI	ACNNNNNCTCC	2 cut(s)	604, 634
Bsc4I	CCNNNNNNNGG	4 cut(s)	86, 223, 376, 1174
Bse118I	RCCGGY	2 cut(s)	20, 1154
Bse1I	ACTGG	4 cut(s)	61, 867, 886, 1106
Bse21I	CCTNAGG	1 cut(s)	1460
BseBI	CCWGG	1 cut(s)	1580
BseDI	CCNNGG	4 cut(s)	114, 322, 370, 383
BseGI	GGATG	7 cut(s)	454, 600, 871, 996, 1330, 1343, 1558
BseLI	CCNNNNNNNGG	4 cut(s)	86, 223, 376, 1174
BseMII	CTCAG	2 cut(s)	1451, 1563
BseNI	ACTGG	4 cut(s)	61, 867, 886, 1106
BseSI	GKGCMC	1 cut(s)	251
BseXI	GCAGC	7 cut(s)	29, 153, 228, 952, 980, 1018, 1492
BseYI	CCCAGC	1 cut(s)	123
BsgI	GTGCAG	2 cut(s)	1017, 1056
Bsh1236I	CGCG	2 cut(s)	581, 1547
BshFI	GGCC	6 cut(s)	8, 44, 320, 365, 719, 1178
BshNI	GGYRCC	1 cut(s)	344
BsiHKAI	GWGCWC	4 cut(s)	251, 1058, 1347, 1578
BsiHKCI	CYCGRG	1 cut(s)	1278
BsiSI	CCGG	7 cut(s)	21, 183, 324, 372, 383, 1130, 1155
BslFI	GGGAC	1 cut(s)	52
BslI	CCNNNNNNNGG	4 cut(s)	86, 223, 376, 1174
BsmAI	GTCTC	4 cut(s)	838, 891, 1163, 1369
BsmFI	GGGAC	1 cut(s)	52
BsnI	GGCC	6 cut(s)	8, 44, 320, 365, 719, 1178
Bso31I	GGTCTC	1 cut(s)	1163
BsoBI	CYCGRG	1 cut(s)	1278
Bsp1286I	GDGCHC	5 cut(s)	251, 1058, 1278, 1347, 1578
Bsp143I	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
Bsp1720I	GCTNAGC	1 cut(s)	1572
BspANI	GGCC	6 cut(s)	8, 44, 320, 365, 719, 1178
BspCNI	CTCAG	2 cut(s)	1452, 1564
BspFNI	CGCG	2 cut(s)	581, 1547
BspHI	TCATGA	1 cut(s)	1050
BspLI	GGNNCC	5 cut(s)	321, 346, 381, 1020, 1543
BspMAI	CTGCAG	1 cut(s)	970
BspOI	GCTAGC	1 cut(s)	1287
BspPI	GGATC	3 cut(s)	193, 1013, 1026
BspQI	GCTCTTC	1 cut(s)	431
BspT107I	GGYRCC	1 cut(s)	344
BspTNI	GGTCTC	1 cut(s)	1163
BsrFI	RCCGGY	2 cut(s)	20, 1154
BsrI	ACTGG	4 cut(s)	61, 867, 886, 1106
BssAI	RCCGGY	2 cut(s)	20, 1154
BssECI	CCNNGG	4 cut(s)	114, 322, 370, 383
BssMI	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
BssNI	GRCGYC	1 cut(s)	345
BssT1I	CCWWGG	1 cut(s)	114
Bst2UI	CCWGG	1 cut(s)	1580
Bst4CI	ACNGT	5 cut(s)	13, 253, 1151, 1223, 1434
Bst6I	CTCTTC	2 cut(s)	431, 1294
BstACI	GRCGYC	1 cut(s)	345
BstAPI	GCANNNNNTGC	1 cut(s)	1079
BstC8I	GCNNGC	3 cut(s)	22, 408, 1285
BstDEI	CTNAG	4 cut(s)	1402, 1460, 1572, 1586
BstF5I	GGATG	7 cut(s)	454, 600, 871, 996, 1330, 1343, 1558
BstFNI	CGCG	2 cut(s)	581, 1547
BstH2I	RGCGCY	2 cut(s)	348, 1290
BstHHI	GCGC	4 cut(s)	347, 581, 1289, 1549
BstKTI	GATC	6 cut(s)	201, 951, 958, 1021, 1294, 1538
BstMAI	GTCTC	4 cut(s)	838, 891, 1163, 1369
BstMBI	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
BstMWI	GCNNNNNNNGC	9 cut(s)	14, 30, 326, 335, 344, 700, 1079, 1088, 1397
BstNI	CCWGG	1 cut(s)	1580
BstNSI	RCATGY	1 cut(s)	400
BstSCI	CCNGG	4 cut(s)	322, 370, 382, 1578
BstSFI	CTRYAG	2 cut(s)	966, 1147
BstSLI	GKGCMC	1 cut(s)	251
BstUI	CGCG	2 cut(s)	581, 1547
BstV1I	GCAGC	7 cut(s)	29, 153, 228, 952, 980, 1018, 1492
BstV2I	GAAGAC	2 cut(s)	519, 863
BstX2I	RGATCY	1 cut(s)	1018
BstXI	CCANNNNNNTGG	1 cut(s)	1113
BstYI	RGATCY	1 cut(s)	1018
Bsu36I	CCTNAGG	1 cut(s)	1460
BsuI	GTATCC	1 cut(s)	1419
BsuRI	GGCC	6 cut(s)	8, 44, 320, 365, 719, 1178
BtsCI	GGATG	7 cut(s)	454, 600, 871, 996, 1330, 1343, 1558
BtsI	GCAGTG	1 cut(s)	1201
BtsIMutI	CAGTG	5 cut(s)	9, 860, 1156, 1201, 1393
Cac8I	GCNNGC	3 cut(s)	22, 408, 1285
CaiI	CAGNNNCTG	2 cut(s)	1459, 1571
CciI	TCATGA	1 cut(s)	1050
CfoI	GCGC	4 cut(s)	347, 581, 1289, 1549
Cfr10I	RCCGGY	2 cut(s)	20, 1154
Cfr13I	GGNCC	4 cut(s)	319, 363, 680, 1582
CseI	GACGC	1 cut(s)	319
Csp6I	GTAC	4 cut(s)	646, 757, 1366, 1596
CviAII	CATG	3 cut(s)	397, 1051, 1257
CviQI	GTAC	4 cut(s)	646, 757, 1366, 1596
DdeI	CTNAG	4 cut(s)	1402, 1460, 1572, 1586
DinI	GGCGCC	1 cut(s)	346
DpnI	GATC	6 cut(s)	200, 950, 957, 1020, 1293, 1537
DpnII	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
DrdI	GACNNNNNNGTC	1 cut(s)	980
DseDI	GACNNNNNNGTC	1 cut(s)	980
EaeI	YGGCCR	3 cut(s)	6, 42, 717
Eam1104I	CTCTTC	2 cut(s)	431, 1294
EarI	CTCTTC	2 cut(s)	431, 1294
EciI	GGCGGA	1 cut(s)	39
Ecl136II	GAGCTC	2 cut(s)	1345, 1576
Eco130I	CCWWGG	1 cut(s)	114
Eco24I	GRGCYC	3 cut(s)	1278, 1347, 1578
Eco31I	GGTCTC	1 cut(s)	1163
Eco32I	GATATC	1 cut(s)	1144
Eco47I	GGWCC	2 cut(s)	680, 1582
Eco47III	AGCGCT	1 cut(s)	1288
Eco53kI	GAGCTC	2 cut(s)	1345, 1576
Eco57I	CTGAAG	2 cut(s)	412, 948
Eco81I	CCTNAGG	1 cut(s)	1460
Eco88I	CYCGRG	1 cut(s)	1278
EcoICRI	GAGCTC	2 cut(s)	1345, 1576
EcoO109I	RGGNCCY	2 cut(s)	680, 1582
EcoRII	CCWGG	1 cut(s)	1578
EcoRV	GATATC	1 cut(s)	1144
EcoT14I	CCWWGG	1 cut(s)	114
EcoT38I	GRGCYC	3 cut(s)	1278, 1347, 1578
EgeI	GGCGCC	1 cut(s)	346
EheI	GGCGCC	1 cut(s)	346
ErhI	CCWWGG	1 cut(s)	114
FaeI	CATG	3 cut(s)	400, 1054, 1260
FaqI	GGGAC	1 cut(s)	52
FatI	CATG	3 cut(s)	396, 1050, 1256
FauNDI	CATATG	1 cut(s)	698
FokI	GGATG	7 cut(s)	461, 607, 878, 983, 1337, 1350, 1565
FriOI	GRGCYC	3 cut(s)	1278, 1347, 1578
FspBI	CTAG	1 cut(s)	1284
GlaI	GCGC	4 cut(s)	346, 580, 1288, 1548
GsaI	CCCAGC	1 cut(s)	127
GsuI	CTGGAG	1 cut(s)	1562
HaeII	RGCGCY	2 cut(s)	348, 1290
HaeIII	GGCC	6 cut(s)	8, 44, 320, 365, 719, 1178
HapII	CCGG	7 cut(s)	21, 183, 324, 372, 383, 1130, 1155
HgaI	GACGC	1 cut(s)	319
HhaI	GCGC	4 cut(s)	347, 581, 1289, 1549
Hin1I	GRCGYC	1 cut(s)	345
Hin1II	CATG	3 cut(s)	400, 1054, 1260
Hin6I	GCGC	4 cut(s)	345, 579, 1287, 1547
HinP1I	GCGC	4 cut(s)	345, 579, 1287, 1547
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HinfI	GANTC	6 cut(s)	119, 141, 775, 825, 981, 1383
HpaII	CCGG	7 cut(s)	21, 183, 324, 372, 383, 1130, 1155
HphI	GGTGA	3 cut(s)	50, 203, 265
Hpy166II	GTNNAC	3 cut(s)	211, 249, 499
Hpy188I	TCNGA	4 cut(s)	107, 301, 431, 928
Hpy188III	TCNNGA	8 cut(s)	37, 138, 656, 791, 946, 1051, 1348, 1539
Hpy8I	GTNNAC	3 cut(s)	211, 249, 499
HpyCH4III	ACNGT	5 cut(s)	13, 253, 1151, 1223, 1434
HpyF10VI	GCNNNNNNNGC	9 cut(s)	14, 30, 326, 335, 344, 700, 1079, 1088, 1397
HpyF3I	CTNAG	4 cut(s)	1402, 1460, 1572, 1586
Hsp92I	GRCGYC	1 cut(s)	345
Hsp92II	CATG	3 cut(s)	400, 1054, 1260
HspAI	GCGC	4 cut(s)	345, 579, 1287, 1547
KasI	GGCGCC	1 cut(s)	344
KroI	GCCGGC	1 cut(s)	20
KroNI	GCCGGC	1 cut(s)	22
Kzo9I	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
LguI	GCTCTTC	1 cut(s)	431
LmnI	GCTCC	7 cut(s)	54, 385, 962, 1087, 1342, 1350, 1581
Lsp1109I	GCAGC	7 cut(s)	29, 153, 228, 952, 980, 1018, 1492
LweI	GCATC	4 cut(s)	42, 726, 996, 1440
MaeI	CTAG	1 cut(s)	1284
MaeIII	GTNAC	1 cut(s)	1196
MalI	GATC	6 cut(s)	200, 950, 957, 1020, 1293, 1537
MboI	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
MboII	GAAGA	7 cut(s)	418, 519, 522, 571, 657, 863, 1311
MflI	RGATCY	1 cut(s)	1018
MhlI	GDGCHC	5 cut(s)	251, 1058, 1278, 1347, 1578
MluCI	AATT	2 cut(s)	1123, 1214
Mly113I	GGCGCC	1 cut(s)	345
MlyI	GAGTC	1 cut(s)	990
MmeI	TCCRAC	1 cut(s)	1296
MnlI	CCTC	7 cut(s)	66, 85, 414, 606, 1295, 1455, 1495
MroNI	GCCGGC	1 cut(s)	20
MseI	TTAA	1 cut(s)	152
MslI	CAYNNNNRTG	2 cut(s)	254, 401
MspA1I	CMGCKG	1 cut(s)	630
MspI	CCGG	7 cut(s)	21, 183, 324, 372, 383, 1130, 1155
MspR9I	CCNGG	4 cut(s)	324, 372, 384, 1580
MvaI	CCWGG	1 cut(s)	1580
MvnI	CGCG	2 cut(s)	581, 1547
MwoI	GCNNNNNNNGC	9 cut(s)	14, 30, 326, 335, 344, 700, 1079, 1088, 1397
NaeI	GCCGGC	1 cut(s)	22
NarI	GGCGCC	1 cut(s)	345
NciI	CCSGG	3 cut(s)	324, 372, 384
NdeI	CATATG	1 cut(s)	698
NdeII	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
NgoMIV	GCCGGC	1 cut(s)	20
NheI	GCTAGC	1 cut(s)	1283
NlaIII	CATG	3 cut(s)	400, 1054, 1260
NlaIV	GGNNCC	5 cut(s)	321, 346, 381, 1020, 1543
NmeAIII	GCCGAG	2 cut(s)	66, 1297
NmuCI	GTSAC	1 cut(s)	1196
NspI	RCATGY	1 cut(s)	400
PagI	TCATGA	1 cut(s)	1050
PciI	ACATGT	1 cut(s)	396
PciSI	GCTCTTC	1 cut(s)	431
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PdiI	GCCGGC	1 cut(s)	22
PfeI	GAWTC	5 cut(s)	119, 141, 775, 825, 1383
PfoI	TCCNGGA	1 cut(s)	1578
PleI	GAGTC	1 cut(s)	989
PluTI	GGCGCC	1 cut(s)	348
PpsI	GAGTC	1 cut(s)	989
PpuMI	RGGWCCY	2 cut(s)	680, 1582
PscI	ACATGT	1 cut(s)	396
Psp124BI	GAGCTC	2 cut(s)	1347, 1578
Psp5II	RGGWCCY	2 cut(s)	680, 1582
Psp6I	CCWGG	1 cut(s)	1578
PspFI	CCCAGC	1 cut(s)	123
PspGI	CCWGG	1 cut(s)	1578
PspN4I	GGNNCC	5 cut(s)	321, 346, 381, 1020, 1543
PspPI	GGNCC	4 cut(s)	319, 363, 680, 1582
PspPPI	RGGWCCY	2 cut(s)	680, 1582
PstI	CTGCAG	1 cut(s)	970
PstNI	CAGNNNCTG	2 cut(s)	1459, 1571
PsuI	RGATCY	1 cut(s)	1018
PvuII	CAGCTG	1 cut(s)	630
RsaI	GTAC	4 cut(s)	647, 758, 1367, 1597
RsaNI	GTAC	4 cut(s)	646, 757, 1366, 1596
RseI	CAYNNNNRTG	2 cut(s)	254, 401
SacI	GAGCTC	2 cut(s)	1347, 1578
SapI	GCTCTTC	1 cut(s)	431
SaqAI	TTAA	1 cut(s)	152
Sau3AI	GATC	6 cut(s)	198, 948, 955, 1018, 1291, 1535
Sau96I	GGNCC	4 cut(s)	319, 363, 680, 1582
SchI	GAGTC	1 cut(s)	990
ScrFI	CCNGG	4 cut(s)	324, 372, 384, 1580
SduI	GDGCHC	5 cut(s)	251, 1058, 1278, 1347, 1578
SfaNI	GCATC	4 cut(s)	42, 726, 996, 1440
SfcI	CTRYAG	2 cut(s)	966, 1147
SfoI	GGCGCC	1 cut(s)	346
SinI	GGWCC	2 cut(s)	680, 1582
SmiMI	CAYNNNNRTG	2 cut(s)	254, 401
SmlI	CTYRAG	1 cut(s)	656
SmoI	CTYRAG	1 cut(s)	656
Sse9I	AATT	2 cut(s)	1123, 1214
SspDI	GGCGCC	1 cut(s)	344
SspI	AATATT	1 cut(s)	689
SspMI	CTAG	1 cut(s)	1284
SstI	GAGCTC	2 cut(s)	1347, 1578
StyD4I	CCNGG	4 cut(s)	322, 370, 382, 1578
StyI	CCWWGG	1 cut(s)	114
TaaI	ACNGT	5 cut(s)	13, 253, 1151, 1223, 1434
TaqI	TCGA	2 cut(s)	137, 1477
TasI	AATT	2 cut(s)	1123, 1214
TauI	GCSGC	5 cut(s)	8, 189, 267, 719, 1271
TfiI	GAWTC	5 cut(s)	119, 141, 775, 825, 1383
Tru1I	TTAA	1 cut(s)	152
Tru9I	TTAA	1 cut(s)	152
TscAI	CASTG	5 cut(s)	16, 867, 1156, 1201, 1393
TseFI	GTSAC	1 cut(s)	1196
TseI	GCWGC	7 cut(s)	17, 166, 241, 965, 968, 1031, 1505
Tsp45I	GTSAC	1 cut(s)	1196
TspDTI	ATGAA	4 cut(s)	498, 611, 863, 1136
TspGWI	ACGGA	2 cut(s)	41, 744
TspRI	CASTG	5 cut(s)	16, 867, 1156, 1201, 1393
VneI	GTGCAC	1 cut(s)	247
VpaK11BI	GGWCC	2 cut(s)	680, 1582
XapI	RAATTY	1 cut(s)	1123
XceI	RCATGY	1 cut(s)	400
XcmI	CCANNNNNNNNNTGG	2 cut(s)	166, 373
XspI	CTAG	1 cut(s)	1284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.