Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0014458

Physical Location & Seq

Genomic Coordinates Forward (+)

57561108 .. 57565830

Gene Structure 4723 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00377000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGCCGTTATAGTCGCAGCTGGAGGAGTTAGTACTATCGGAGTTGCCAAGCTCCACCCTCACCAGTCAAAACCCACATCCCATTTTCTAGGCAGGAAGCTCAGAATAACCAAACCCTCCGGTTTAGTAAAAACCAGCTCAAACCCTAGAGCCTTGGTGGTCTTGGCCTCCGGCGGGGAGCTGATCCATGCGGTGCACGACCTGTTTCTAGGGGTGGGGGTGGGGCTGCCCTGCACGGTCATGGAGTGCGGTGATATGATCTACAGAAGCACTCTTCCCAAGTCGAATGCCTTGACGATCACGGTACCCGGTGCGATTCTGGCTCTGGGGGCTCTGTCTTATCTCTGGGCCACTCCAGGTGTGGCTCCGGGGTTCTTTGACATGTTTGTGCTTGCTTTTGTTGAGAGGATTTTCAGACCCACTTACAAGAAGGGTGACTTTGTTTTGGGGAAGAAGTTGGGTGAAGGAGCTTTTGGAGTGGTGTACAAAGTTTCATTGGCTAACAAGAAGGCCAAGAAAGAAGAGGAATTGGTCTTGAAAAAGGCTACTGAATATGGCGCGGTAGAGATTTGGATGAATGAGCGTGTGCGAAGGGCATGTGCAAACAGCTGTGCTGATTTTAAGTACGGCTTCCTTGAGAGTTCTTCAAAGAAAGAAGCTGAATACTGGCTTATATGGCGGTTTGAAGGGGAAACCACACTTTATGATTTGATGCAAAGCAAAGACTTTCCCTACAATGTGGAAACTATGATACTTGGAAAAGTTCAGGACTTGCCAAAGGGTCTGGAAAGGGAAAATAGAATCATTCAAACAATAATGAGACAGATCTTATTTGCATTGGATGGTCTTCACTCAACAGGGATTGTGCACAGAGATATAAAGCCTCAAAATATTATTTTCTCTGAAGGCTCTCGTACATTCAAGATCATTGACCTTGGGGCTGCAGCAGATTTGCGAGTCGGCATCAACTACATTCCAAAGGAGTTTCTTTTGGATCCAAGATATGCTGCACCTGAGCAATACATCATGAGCACACAGACACCATCTGCACCCTCTGCTCCAGTGGCAACAGCACTTTCCCCAGTTCTATGGCAGATGAATTTACCCGATAGATTTGATATCTACAGTACTGGGCTCATTTTCCTACAAATGGCATTCCCAGGATTACGTAGTGACAGCGGCCTGATACAATTCAACCGTCAGCTTAAAAGGTGTGATTATGACTTGGTTGCATGGAGGAAAACTGCTGAGCCTAGAGCTAGCTCTGAACTACGAAAAGGTTTTGAGTTGTTGGATTTAGATGGTGGAATTGGATGGGAACTCCTGACATCAATGGTTCGATACAAACCAAGACAAAGAATCAGTGCAAAAACAGCTTTAGCATATCCCTACTTTGACAAGGAAGGCCTGTTAGCGCTGTCCGTCATGCAGAGTCTGAGGCTGCAACTCTTCCGATACACACAACAAGATTATGGGGAGGCTGCCAGATGGGTTACTCGGCTAATGGCAAGATCCGGAACCAAGCAGGATGGTGGCTTCACAGAAGCTCAACTCCAGGAGCTTATGGAAACGAAGCCTAAGAAGAAGTCTAGTCCTCGGAGAAATGCTCTTGCTTCAGCTCTTTGGCTGCAGAGGAAGATTGTGAAAACATTGAATGAGAGCATGAATGAGCTTAACAACCAAAGGAAGAGTCTCTGGTGGATCAGGTGGATTCCCAAAGAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009643 GO:0009987 GO:0010109 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0031323 GO:0034357 GO:0036211 GO:0042548 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043467 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

575

Amino Acids

64.67

Weight (kDa)

9.56

Isoelectric Point (pI)

41.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	148 - 340	1.5e-19	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	149 - 340	4.6e-10	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014458)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68830
fragaria_vesca	FvH4_4g28080
malus_domestica	MD03G1264300.v1.1 MD13G1046400.v1.1
prunus_persica	Prupe.1G305500_v2.0.a1
pyrus_communis	pycom13g04070
rosa_chinensis	RchiOBHm_Chr4g0435821
rosa_laevigata	RLG00000006556
rosa_multiflora	Rmu_sc0002642.1_g000004 Rmu_sc0010838.1_g000002
rosa_roxburghii	Rroxscaffold_5G00377000
rosa_rugosa	Rorug04G0289800
rosa_samantha	Rh4AG344100 Rh4BG353000 Rh4CG367400 Rh4DG346900
rosa_wichuraiana	Rw4G030060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	304
AccB1I	GGYRCC	1 cut(s)	304
AccII	CGCG	1 cut(s)	560
AccIII	TCCGGA	1 cut(s)	1514
AciI	CCGC	6 cut(s)	174, 191, 249, 560, 679, 1179
AclWI	GGATC	5 cut(s)	178, 989, 1002, 1506, 1709
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	1099
AcuI	CTGAAG	2 cut(s)	924, 1599
AfaI	GTAC	6 cut(s)	34, 306, 485, 626, 916, 1129
AfeI	AGCGCT	1 cut(s)	1416
AfiI	CCNNNNNNNGG	2 cut(s)	174, 1150
AflIII	ACRYGT	1 cut(s)	381
AgsI	TTSAA	7 cut(s)	538, 648, 686, 809, 922, 1195, 1654
AjnI	CCWGG	3 cut(s)	355, 1159, 1554
AjuI	GAANNNNNNNTTGG	2 cut(s)	456, 488
Alw21I	GWGCWC	3 cut(s)	198, 870, 1034
Alw26I	GTCTC	2 cut(s)	814, 1697
Alw44I	GTGCAC	2 cut(s)	194, 866
AlwI	GGATC	5 cut(s)	178, 989, 1002, 1506, 1709
AlwNI	CAGNNNCTG	1 cut(s)	1435
Aor13HI	TCCGGA	1 cut(s)	1514
Aor51HI	AGCGCT	1 cut(s)	1416
AoxI	GGCC	6 cut(s)	3, 165, 348, 510, 1180, 1405
ApaLI	GTGCAC	2 cut(s)	194, 866
ApeKI	GCWGC	8 cut(s)	17, 226, 941, 944, 1007, 1441, 1481, 1627
ApoI	RAATTY	1 cut(s)	1099
ArsI	GACNNNNNNTTYG	2 cut(s)	1675, 1707
Asp718I	GGTACC	1 cut(s)	304
AspLEI	GCGC	2 cut(s)	560, 1417
AspS9I	GGNCC	1 cut(s)	348
AsuC2I	CCSGG	2 cut(s)	309, 369
AsuHPI	GGTGA	4 cut(s)	53, 263, 446, 473
AsuNHI	GCTAGC	1 cut(s)	1259
BaeGI	GKGCMC	2 cut(s)	198, 870
BamHI	GGATCC	1 cut(s)	994
BanI	GGYRCC	1 cut(s)	304
BanII	GRGCYC	2 cut(s)	334, 1137
BbsI	GAAGAC	1 cut(s)	839
Bbv12I	GWGCWC	3 cut(s)	198, 870, 1034
BbvI	GCAGC	8 cut(s)	29, 213, 928, 956, 994, 1428, 1468, 1614
BccI	CCATC	6 cut(s)	836, 1051, 1295, 1308, 1482, 1523
BceAI	ACGGC	1 cut(s)	643
BciT130I	CCWGG	3 cut(s)	357, 1161, 1556
BcnI	CCSGG	2 cut(s)	309, 369
BcoDI	GTCTC	2 cut(s)	814, 1697
BfaI	CTAG	6 cut(s)	89, 147, 209, 1254, 1260, 1590
BfmI	CTRYAG	4 cut(s)	262, 942, 1123, 1628
BfoI	RGCGCY	1 cut(s)	1418
BglII	AGATCT	1 cut(s)	825
BisI	GCNGC	9 cut(s)	18, 227, 942, 945, 1008, 1180, 1442, 1482, 1628
BlpI	GCTNAGC	1 cut(s)	1248
BlsI	GCNGC	9 cut(s)	19, 228, 943, 946, 1009, 1181, 1443, 1483, 1629
BmcAI	AGTACT	2 cut(s)	34, 1129
Bme1390I	CCNGG	5 cut(s)	309, 357, 369, 1161, 1556
BmgT120I	GGNCC	1 cut(s)	348
BmiI	GGNNCC	4 cut(s)	306, 366, 996, 1519
BmrFI	CCNGG	5 cut(s)	309, 357, 369, 1161, 1556
BmrI	ACTGGG	2 cut(s)	1076, 1140
BmsI	GCATC	2 cut(s)	702, 972
BmtI	GCTAGC	1 cut(s)	1263
BmuI	ACTGGG	2 cut(s)	1076, 1140
BpiI	GAAGAC	1 cut(s)	839
BplI	GAGNNNNNCTC	2 cut(s)	1591, 1623
BpmI	CTGGAG	4 cut(s)	42, 339, 1044, 1538
Bpu10I	CCTNAGC	1 cut(s)	1014
Bpu1102I	GCTNAGC	1 cut(s)	1248
BpuEI	CTTGAG	1 cut(s)	656
BpuMI	CCSGG	2 cut(s)	309, 369
BsaAI	YACGTR	1 cut(s)	1169
BsaJI	CCNNGG	5 cut(s)	153, 368, 934, 1159, 1595
BsaWI	WCCGGW	2 cut(s)	119, 1514
Bsc4I	CCNNNNNNNGG	2 cut(s)	174, 1150
Bse1I	ACTGG	5 cut(s)	64, 671, 1061, 1082, 1135
BseAI	TCCGGA	1 cut(s)	1514
BseBI	CCWGG	3 cut(s)	357, 1161, 1556
BseDI	CCNNGG	5 cut(s)	153, 368, 934, 1159, 1595
BseGI	GGATG	5 cut(s)	77, 579, 847, 1319, 1534
BseLI	CCNNNNNNNGG	2 cut(s)	174, 1150
BseMII	CTCAG	4 cut(s)	115, 1005, 1239, 1427
BseNI	ACTGG	5 cut(s)	64, 671, 1061, 1082, 1135
BseRI	GAGGAG	1 cut(s)	39
BseSI	GKGCMC	2 cut(s)	198, 870
BseXI	GCAGC	8 cut(s)	29, 213, 928, 956, 994, 1428, 1468, 1614
BsgI	GTGCAG	3 cut(s)	217, 993, 1032
Bsh1236I	CGCG	1 cut(s)	560
BshFI	GGCC	6 cut(s)	5, 167, 350, 512, 1182, 1407
BshNI	GGYRCC	1 cut(s)	304
BsiHKAI	GWGCWC	3 cut(s)	198, 870, 1034
BsiSI	CCGG	5 cut(s)	120, 171, 309, 368, 1515
BslI	CCNNNNNNNGG	2 cut(s)	174, 1150
BsmAI	GTCTC	2 cut(s)	814, 1697
BsmI	GAATGC	2 cut(s)	292, 1154
BsnI	GGCC	6 cut(s)	5, 167, 350, 512, 1182, 1407
Bsp1286I	GDGCHC	5 cut(s)	198, 334, 870, 1034, 1137
Bsp13I	TCCGGA	1 cut(s)	1514
Bsp1407I	TGTACA	1 cut(s)	483
Bsp143I	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
Bsp1720I	GCTNAGC	1 cut(s)	1248
BspACI	CCGC	6 cut(s)	174, 191, 249, 560, 679, 1179
BspANI	GGCC	6 cut(s)	5, 167, 350, 512, 1182, 1407
BspCNI	CTCAG	4 cut(s)	114, 1006, 1240, 1428
BspEI	TCCGGA	1 cut(s)	1514
BspFNI	CGCG	1 cut(s)	560
BspHI	TCATGA	1 cut(s)	1026
BspLI	GGNNCC	4 cut(s)	306, 366, 996, 1519
BspMAI	CTGCAG	2 cut(s)	946, 1632
BspOI	GCTAGC	1 cut(s)	1263
BspPI	GGATC	5 cut(s)	178, 989, 1002, 1506, 1709
BspT107I	GGYRCC	1 cut(s)	304
BsrGI	TGTACA	1 cut(s)	483
BsrI	ACTGG	5 cut(s)	64, 671, 1061, 1082, 1135
BssECI	CCNNGG	5 cut(s)	153, 368, 934, 1159, 1595
BssMI	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
BssT1I	CCWWGG	2 cut(s)	153, 934
Bst2UI	CCWGG	3 cut(s)	357, 1161, 1556
Bst4CI	ACNGT	4 cut(s)	238, 304, 1127, 1199
Bst6I	CTCTTC	4 cut(s)	279, 516, 1454, 1682
BstAPI	GCANNNNNTGC	1 cut(s)	1055
BstAUI	TGTACA	1 cut(s)	483
BstBAI	YACGTR	1 cut(s)	1169
BstC8I	GCNNGC	2 cut(s)	393, 1261
BstDEI	CTNAG	5 cut(s)	101, 1014, 1248, 1436, 1578
BstF5I	GGATG	5 cut(s)	77, 579, 847, 1319, 1534
BstFNI	CGCG	1 cut(s)	560
BstH2I	RGCGCY	1 cut(s)	1418
BstHHI	GCGC	2 cut(s)	560, 1417
BstKTI	GATC	8 cut(s)	186, 261, 300, 828, 927, 997, 1514, 1704
BstMAI	GTCTC	2 cut(s)	814, 1697
BstMBI	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
BstMWI	GCNNNNNNNGC	6 cut(s)	320, 329, 676, 1055, 1064, 1373
BstNI	CCWGG	3 cut(s)	357, 1161, 1556
BstNSI	RCATGY	2 cut(s)	385, 600
BstSCI	CCNGG	5 cut(s)	307, 355, 367, 1159, 1554
BstSFI	CTRYAG	4 cut(s)	262, 942, 1123, 1628
BstSLI	GKGCMC	2 cut(s)	198, 870
BstSNI	TACGTA	1 cut(s)	1169
BstUI	CGCG	1 cut(s)	560
BstV1I	GCAGC	8 cut(s)	29, 213, 928, 956, 994, 1428, 1468, 1614
BstV2I	GAAGAC	1 cut(s)	839
BstX2I	RGATCY	3 cut(s)	825, 994, 1511
BstXI	CCANNNNNNTGG	1 cut(s)	1089
BstYI	RGATCY	3 cut(s)	825, 994, 1511
BsuRI	GGCC	6 cut(s)	5, 167, 350, 512, 1182, 1407
BtsCI	GGATG	5 cut(s)	77, 579, 847, 1319, 1534
BtsIMutI	CAGTG	2 cut(s)	1068, 1369
Cac8I	GCNNGC	2 cut(s)	393, 1261
CaiI	CAGNNNCTG	1 cut(s)	1435
CciI	TCATGA	1 cut(s)	1026
CfoI	GCGC	2 cut(s)	560, 1417
Cfr13I	GGNCC	1 cut(s)	348
Csp6I	GTAC	6 cut(s)	33, 305, 484, 625, 915, 1128
CviAII	CATG	8 cut(s)	188, 241, 382, 597, 1027, 1233, 1426, 1663
CviQI	GTAC	6 cut(s)	33, 305, 484, 625, 915, 1128
DdeI	CTNAG	5 cut(s)	101, 1014, 1248, 1436, 1578
DpnI	GATC	8 cut(s)	185, 260, 299, 827, 926, 996, 1513, 1703
DpnII	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	4 cut(s)	279, 516, 1454, 1682
EarI	CTCTTC	4 cut(s)	279, 516, 1454, 1682
Eco105I	TACGTA	1 cut(s)	1169
Eco130I	CCWWGG	2 cut(s)	153, 934
Eco147I	AGGCCT	1 cut(s)	1407
Eco24I	GRGCYC	2 cut(s)	334, 1137
Eco32I	GATATC	1 cut(s)	1120
Eco47III	AGCGCT	1 cut(s)	1416
Eco57I	CTGAAG	2 cut(s)	924, 1599
EcoRII	CCWGG	3 cut(s)	355, 1159, 1554
EcoRV	GATATC	1 cut(s)	1120
EcoT14I	CCWWGG	2 cut(s)	153, 934
EcoT38I	GRGCYC	2 cut(s)	334, 1137
ErhI	CCWWGG	2 cut(s)	153, 934
FaeI	CATG	8 cut(s)	191, 244, 385, 600, 1030, 1236, 1429, 1666
FalI	AAGNNNNNCTT	4 cut(s)	422, 454, 614, 646
FatI	CATG	8 cut(s)	187, 240, 381, 596, 1026, 1232, 1425, 1662
FauI	CCCGC	1 cut(s)	167
Fnu4HI	GCNGC	9 cut(s)	18, 227, 942, 945, 1008, 1180, 1442, 1482, 1628
FokI	GGATG	5 cut(s)	64, 586, 854, 1326, 1541
FriOI	GRGCYC	2 cut(s)	334, 1137
Fsp4HI	GCNGC	9 cut(s)	18, 227, 942, 945, 1008, 1180, 1442, 1482, 1628
FspBI	CTAG	6 cut(s)	89, 147, 209, 1254, 1260, 1590
GlaI	GCGC	2 cut(s)	559, 1416
GluI	GCNGC	9 cut(s)	18, 227, 942, 945, 1008, 1180, 1442, 1482, 1628
GsuI	CTGGAG	4 cut(s)	42, 339, 1044, 1538
HaeII	RGCGCY	1 cut(s)	1418
HaeIII	GGCC	6 cut(s)	5, 167, 350, 512, 1182, 1407
HapII	CCGG	5 cut(s)	120, 171, 309, 368, 1515
HhaI	GCGC	2 cut(s)	560, 1417
Hin1II	CATG	8 cut(s)	191, 244, 385, 600, 1030, 1236, 1429, 1666
Hin6I	GCGC	2 cut(s)	558, 1415
HinP1I	GCGC	2 cut(s)	558, 1415
HinfI	GANTC	7 cut(s)	316, 801, 957, 1359, 1432, 1690, 1711
HpaII	CCGG	5 cut(s)	120, 171, 309, 368, 1515
HphI	GGTGA	4 cut(s)	53, 263, 446, 473
Hpy166II	GTNNAC	3 cut(s)	196, 484, 868
Hpy188I	TCNGA	8 cut(s)	41, 104, 416, 904, 1267, 1437, 1454, 1599
Hpy188III	TCNNGA	7 cut(s)	535, 767, 785, 922, 1027, 1324, 1515
Hpy8I	GTNNAC	3 cut(s)	196, 484, 868
HpyAV	CCTTC	7 cut(s)	424, 458, 502, 585, 680, 899, 1397
HpyCH4III	ACNGT	4 cut(s)	238, 304, 1127, 1199
HpyCH4IV	ACGT	1 cut(s)	1168
HpyF10VI	GCNNNNNNNGC	6 cut(s)	320, 329, 676, 1055, 1064, 1373
HpyF3I	CTNAG	5 cut(s)	101, 1014, 1248, 1436, 1578
HpySE526I	ACGT	1 cut(s)	1168
Hsp92II	CATG	8 cut(s)	191, 244, 385, 600, 1030, 1236, 1429, 1666
HspAI	GCGC	2 cut(s)	558, 1415
Kpn2I	TCCGGA	1 cut(s)	1514
KpnI	GGTACC	1 cut(s)	308
Kzo9I	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
LmnI	GCTCC	6 cut(s)	57, 178, 370, 467, 1063, 1558
Lsp1109I	GCAGC	8 cut(s)	29, 213, 928, 956, 994, 1428, 1468, 1614
LweI	GCATC	2 cut(s)	702, 972
MaeI	CTAG	6 cut(s)	89, 147, 209, 1254, 1260, 1590
MaeII	ACGT	1 cut(s)	1168
MaeIII	GTNAC	3 cut(s)	434, 1172, 1492
MalI	GATC	8 cut(s)	185, 260, 299, 827, 926, 996, 1513, 1703
MboI	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
MboII	GAAGA	9 cut(s)	266, 463, 533, 636, 839, 1441, 1594, 1648, 1699
MflI	RGATCY	3 cut(s)	825, 994, 1511
MhlI	GDGCHC	5 cut(s)	198, 334, 870, 1034, 1137
MluCI	AATT	4 cut(s)	527, 1099, 1190, 1308
MlyI	GAGTC	3 cut(s)	966, 1441, 1699
MmeI	TCCRAC	1 cut(s)	1272
MroI	TCCGGA	1 cut(s)	1514
MseI	TTAA	3 cut(s)	621, 1206, 1674
MslI	CAYNNNNRTG	2 cut(s)	239, 386
MspA1I	CMGCKG	3 cut(s)	20, 609, 1179
MspI	CCGG	5 cut(s)	120, 171, 309, 368, 1515
MspR9I	CCNGG	5 cut(s)	309, 357, 369, 1161, 1556
Mva1269I	GAATGC	2 cut(s)	292, 1154
MvaI	CCWGG	3 cut(s)	357, 1161, 1556
MvnI	CGCG	1 cut(s)	560
MwoI	GCNNNNNNNGC	6 cut(s)	320, 329, 676, 1055, 1064, 1373
NciI	CCSGG	2 cut(s)	309, 369
NdeII	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
NheI	GCTAGC	1 cut(s)	1259
NlaIII	CATG	8 cut(s)	191, 244, 385, 600, 1030, 1236, 1429, 1666
NlaIV	GGNNCC	4 cut(s)	306, 366, 996, 1519
NmeAIII	GCCGAG	1 cut(s)	1477
NmuCI	GTSAC	2 cut(s)	434, 1172
NspI	RCATGY	2 cut(s)	385, 600
PagI	TCATGA	1 cut(s)	1026
PceI	AGGCCT	1 cut(s)	1407
PciI	ACATGT	1 cut(s)	381
PctI	GAATGC	2 cut(s)	292, 1154
PfeI	GAWTC	4 cut(s)	316, 801, 1359, 1711
PfoI	TCCNGGA	1 cut(s)	1554
PkrI	GCNGC	9 cut(s)	19, 228, 943, 946, 1009, 1181, 1443, 1483, 1629
PleI	GAGTC	3 cut(s)	965, 1440, 1698
PpsI	GAGTC	3 cut(s)	965, 1440, 1698
Ppu21I	YACGTR	1 cut(s)	1169
PscI	ACATGT	1 cut(s)	381
Psp6I	CCWGG	3 cut(s)	355, 1159, 1554
PspGI	CCWGG	3 cut(s)	355, 1159, 1554
PspN4I	GGNNCC	4 cut(s)	306, 366, 996, 1519
PspPI	GGNCC	1 cut(s)	348
PstI	CTGCAG	2 cut(s)	946, 1632
PstNI	CAGNNNCTG	1 cut(s)	1435
PsuI	RGATCY	3 cut(s)	825, 994, 1511
PvuII	CAGCTG	2 cut(s)	20, 609
RsaI	GTAC	6 cut(s)	34, 306, 485, 626, 916, 1129
RsaNI	GTAC	6 cut(s)	33, 305, 484, 625, 915, 1128
RseI	CAYNNNNRTG	2 cut(s)	239, 386
SaqAI	TTAA	3 cut(s)	621, 1206, 1674
SatI	GCNGC	9 cut(s)	18, 227, 942, 945, 1008, 1180, 1442, 1482, 1628
Sau3AI	GATC	8 cut(s)	183, 258, 297, 825, 924, 994, 1511, 1701
Sau96I	GGNCC	1 cut(s)	348
ScaI	AGTACT	2 cut(s)	34, 1129
SchI	GAGTC	3 cut(s)	966, 1441, 1699
ScrFI	CCNGG	5 cut(s)	309, 357, 369, 1161, 1556
SduI	GDGCHC	5 cut(s)	198, 334, 870, 1034, 1137
SfaNI	GCATC	2 cut(s)	702, 972
SfcI	CTRYAG	4 cut(s)	262, 942, 1123, 1628
SmiMI	CAYNNNNRTG	2 cut(s)	239, 386
SmlI	CTYRAG	1 cut(s)	635
SmoI	CTYRAG	1 cut(s)	635
SnaBI	TACGTA	1 cut(s)	1169
Sse9I	AATT	4 cut(s)	527, 1099, 1190, 1308
SseBI	AGGCCT	1 cut(s)	1407
SsiI	CCGC	6 cut(s)	174, 191, 249, 560, 679, 1179
SspI	AATATT	1 cut(s)	892
SspMI	CTAG	6 cut(s)	89, 147, 209, 1254, 1260, 1590
StuI	AGGCCT	1 cut(s)	1407
StyD4I	CCNGG	5 cut(s)	307, 355, 367, 1159, 1554
StyI	CCWWGG	2 cut(s)	153, 934
TaaI	ACNGT	4 cut(s)	238, 304, 1127, 1199
TaiI	ACGT	1 cut(s)	1171
TaqI	TCGA	2 cut(s)	284, 1339
TasI	AATT	4 cut(s)	527, 1099, 1190, 1308
TatI	WGTACW	3 cut(s)	32, 483, 1127
TauI	GCSGC	1 cut(s)	1182
TfiI	GAWTC	4 cut(s)	316, 801, 1359, 1711
Tru1I	TTAA	3 cut(s)	621, 1206, 1674
Tru9I	TTAA	3 cut(s)	621, 1206, 1674
TscAI	CASTG	2 cut(s)	1068, 1369
TseFI	GTSAC	2 cut(s)	434, 1172
TseI	GCWGC	8 cut(s)	17, 226, 941, 944, 1007, 1441, 1481, 1627
Tsp45I	GTSAC	2 cut(s)	434, 1172
TspDTI	ATGAA	4 cut(s)	483, 590, 1112, 1679
TspGWI	ACGGA	1 cut(s)	1411
TspRI	CASTG	2 cut(s)	1068, 1369
VneI	GTGCAC	2 cut(s)	194, 866
XapI	RAATTY	1 cut(s)	1099
XceI	RCATGY	2 cut(s)	385, 600
XcmI	CCANNNNNNNNNTGG	1 cut(s)	358
XspI	CTAG	6 cut(s)	89, 147, 209, 1254, 1260, 1590
ZrmI	AGTACT	2 cut(s)	34, 1129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.