Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014027

Physical Location & Seq

Genomic Coordinates Reverse (-)

35404994 .. 35408690

Gene Structure 3697 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1270100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGGCTGCGGTCGCTCTCAGAAACCCTAGCTCCAAGCGCATCCTCCCCTACTCCTCGCAAGTCTACTTGTGCAGCCGAGGATCGATCGCCGCCGCCGGTTCTCCAATCTCCGACTCCGCCTTCGGAAATGACCGATCTTGTAGCCCTTGTCCTTGGTGGCGATCCATGGCCACCTTCACACGCACAAAACCTCATGTAAATGTTGGAACAATTGGACACGTAGATCATGGAAAAACGACGCTGACTGCAGCAATTACAAAGGTACTGGCAGAAGAAGGGAAAGCTAAGGCCGTGGCCTTTGACGAAATTGATAAGGCCCCTGAGGAGAAAAAGAGAGGAATTACAATTGCAACGGCTCATGTAGAATACGAGACTGTTAAGCGCCATTATGCACACGTGGACTGCCCCGGACATGCAGATTATGTCAAAAACATGATTACTGGAGCTGCCCAAATGGATGGAGGTATTCTGGTCGTATCTGCTCCTGATGGGCCTATGCCACAAACTAAGGAACACATTCTGCTCGCCCGCCAAGTTGGTGTGCCATCACTTGTTTGTTTTCTCAATAAAGTTGATGCTGTTGATGATCCAGAGTTGATAGAGCTGGTTGAAATGGAGCTCCGTGAGCTTCTTAGCTTCTACAAGTTCCCTGGGGATGAAATCCCTATCGTCCGGGGTTCGGCTCTATCGGCTTTACAAGGCACAAATGATGAGATTGGCAAAAAAGCAATCTTAAAATTAATGGAATCTGTGGATGAGTACATTCCTGACCCTGTACGCCAGCTTGACAAACCCTTCCTGATGCCAATTGAAGATGTTTTCTCAATTCAAGGTCGTGGAACTGTTGCCACTGGCCGTGTTGAACAAGGGACCATTAAAGTTGGTGAGGAAGTTGAGATTCTGGGGTTACATCAGGGTGCTCCTTTGAAAACAGTGGTGACCGGTGTTGAGATGTTCAAGAAAATCTTGGATCATGGGCAAGCTGGTGATAATGTCGGTCTTCTTCTTAGAGGTCTAAAGAGGGAAGATATCCAACGAGGACAGGTAATTGCCAAGCCAGGAACCGTGAAGACACACAAGAGGTTTGAAGCAGAGATATACGTCCTCACAAAGGATGAAGGTGGACGCCACACTGCCTTTTTCTCAAACTACAGGCCTCAGTTTTATTTGAGGACAGCAGATATTACTGGAAAGGTAGAATTGCCCGAAAATGTTAAGATGGTTATGCCAGGAGACAACGTGACTGCAGTTTTTGAGCTGATTCAGCCGGTTCCTCTTGAACAAGGACAAAGATTTGCCTTGAGGGAGGGAGGAAGAACAGTCGGTGCAGGAGTTGTTTCCAAGGTACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008144 GO:0008150 GO:0008270 GO:0010035 GO:0010038 GO:0017076 GO:0030312 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0050897 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

451

Amino Acids

49.06

Weight (kDa)

6.66

Isoelectric Point (pI)

40.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	63 - 256	5.5e-62	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	280 - 351	4.1e-19	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	355 - 449	5.9e-38	Elongation factor Tu C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02930
fragaria_vesca	FvH4_2g24070
malus_domestica	MD03G1270100.v1.1 MD11G1290900.v1.1
prunus_persica	Prupe.8G246000_v2.0.a1
pyrus_communis	pycom03g21520 pycom11g25790
rosa_chinensis	RchiOBHm_Chr6g0291851
rosa_laevigata	RLG00000012083
rosa_multiflora	Rmu_sc0001043.1_g000017
rosa_roxburghii	Rroxscaffold_7G00175700
rosa_rugosa	Rorug06G0221700
rosa_samantha	Rh6AG334700 Rh6BG342200 Rh6DG335000
rosa_wichuraiana	Rw6G028930 Rw6G029130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	63
AciI	CCGC	5 cut(s)	8, 90, 93, 117, 529
AclWI	GGATC	4 cut(s)	88, 156, 581, 978
AcoI	YGGCCR	2 cut(s)	168, 853
AcvI	CACGTG	1 cut(s)	397
AcyI	GRCGYC	1 cut(s)	1126
AfaI	GTAC	4 cut(s)	264, 761, 777, 1347
AfiI	CCNNNNNNNGG	2 cut(s)	679, 1111
AflIII	ACRYGT	2 cut(s)	217, 394
AgeI	ACCGGT	1 cut(s)	941
AgsI	TTSAA	8 cut(s)	611, 812, 830, 863, 928, 958, 1088, 1280
AjnI	CCWGG	3 cut(s)	649, 1057, 1228
Alw21I	GWGCWC	2 cut(s)	621, 922
Alw26I	GTCTC	2 cut(s)	365, 1227
AlwI	GGATC	4 cut(s)	88, 156, 581, 978
AoxI	GGCC	7 cut(s)	168, 288, 294, 315, 491, 853, 1154
ApeKI	GCWGC	4 cut(s)	5, 72, 248, 446
ArsI	GACNNNNNNTTYG	2 cut(s)	104, 136
AseI	ATTAAT	1 cut(s)	740
AsiGI	ACCGGT	1 cut(s)	941
Asp700I	GAANNNNTTC	1 cut(s)	516
AspLEI	GCGC	2 cut(s)	39, 384
AspS9I	GGNCC	3 cut(s)	316, 491, 870
AsuC2I	CCSGG	2 cut(s)	408, 674
AsuHPI	GGTGA	3 cut(s)	896, 949, 998
AvaII	GGWCC	1 cut(s)	870
AxyI	CCTNAGG	1 cut(s)	321
BalI	TGGCCA	1 cut(s)	170
BanII	GRGCYC	1 cut(s)	621
BbrPI	CACGTG	1 cut(s)	397
BbsI	GAAGAC	2 cut(s)	992, 1076
Bbv12I	GWGCWC	2 cut(s)	621, 922
BbvI	GCAGC	3 cut(s)	84, 260, 433
BccI	CCATC	4 cut(s)	452, 482, 553, 1213
BceAI	ACGGC	3 cut(s)	275, 369, 840
BciT130I	CCWGG	3 cut(s)	651, 1059, 1230
BcnI	CCSGG	2 cut(s)	408, 674
BcoDI	GTCTC	2 cut(s)	365, 1227
BfaI	CTAG	1 cut(s)	27
BfmI	CTRYAG	3 cut(s)	246, 1150, 1245
BfoI	RGCGCY	1 cut(s)	385
BisI	GCNGC	6 cut(s)	6, 73, 90, 93, 249, 447
BlsI	GCNGC	6 cut(s)	7, 74, 91, 94, 250, 448
Bme1390I	CCNGG	5 cut(s)	408, 651, 674, 1059, 1230
Bme18I	GGWCC	1 cut(s)	870
BmgT120I	GGNCC	3 cut(s)	316, 491, 870
BmiI	GGNNCC	4 cut(s)	318, 871, 1063, 1272
BmrFI	CCNGG	5 cut(s)	408, 651, 674, 1059, 1230
BmsI	GCATC	3 cut(s)	48, 565, 792
BpiI	GAAGAC	2 cut(s)	992, 1076
BpmI	CTGGAG	1 cut(s)	462
Bpu10I	CCTNAGC	1 cut(s)	285
BpuEI	CTTGAG	1 cut(s)	1321
BpuMI	CCSGG	2 cut(s)	408, 674
Bsa29I	ATCGAT	1 cut(s)	83
BsaAI	YACGTR	2 cut(s)	220, 397
BsaHI	GRCGYC	1 cut(s)	1126
BsaJI	CCNNGG	9 cut(s)	76, 152, 165, 291, 406, 649, 650, 673, 1341
BsaWI	WCCGGW	1 cut(s)	941
BsaXI	ACNNNNNCTCC	4 cut(s)	14, 44, 1224, 1254
Bsc4I	CCNNNNNNNGG	2 cut(s)	679, 1111
Bse118I	RCCGGY	3 cut(s)	95, 941, 1267
Bse1I	ACTGG	4 cut(s)	270, 445, 856, 1192
Bse21I	CCTNAGG	1 cut(s)	321
BseBI	CCWGG	3 cut(s)	651, 1059, 1230
BseCI	ATCGAT	1 cut(s)	83
BseDI	CCNNGG	9 cut(s)	76, 152, 165, 291, 406, 649, 650, 673, 1341
BseGI	GGATG	5 cut(s)	39, 463, 661, 760, 1120
BseLI	CCNNNNNNNGG	2 cut(s)	679, 1111
BseMII	CTCAG	3 cut(s)	31, 312, 1172
BseNI	ACTGG	4 cut(s)	270, 445, 856, 1192
BseRI	GAGGAG	2 cut(s)	43, 338
BseXI	GCAGC	3 cut(s)	84, 260, 433
BsgI	GTGCAG	2 cut(s)	91, 1347
Bsh1285I	CGRYCG	2 cut(s)	12, 87
BshFI	GGCC	7 cut(s)	170, 290, 296, 317, 493, 855, 1156
BshTI	ACCGGT	1 cut(s)	941
BshVI	ATCGAT	1 cut(s)	83
BsiEI	CGRYCG	2 cut(s)	12, 87
BsiHKAI	GWGCWC	2 cut(s)	621, 922
BsiSI	CCGG	5 cut(s)	96, 408, 673, 942, 1268
BslFI	GGGAC	1 cut(s)	883
BslI	CCNNNNNNNGG	2 cut(s)	679, 1111
BsmAI	GTCTC	2 cut(s)	365, 1227
BsmFI	GGGAC	1 cut(s)	883
BsnI	GGCC	7 cut(s)	170, 290, 296, 317, 493, 855, 1156
Bsp1286I	GDGCHC	2 cut(s)	621, 922
Bsp143I	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
Bsp19I	CCATGG	1 cut(s)	165
BspACI	CCGC	5 cut(s)	8, 90, 93, 117, 529
BspANI	GGCC	7 cut(s)	170, 290, 296, 317, 493, 855, 1156
BspCNI	CTCAG	3 cut(s)	30, 313, 1171
BspDI	ATCGAT	1 cut(s)	83
BspLI	GGNNCC	4 cut(s)	318, 871, 1063, 1272
BspMAI	CTGCAG	2 cut(s)	250, 1249
BspPI	GGATC	4 cut(s)	88, 156, 581, 978
BsrFI	RCCGGY	3 cut(s)	95, 941, 1267
BsrI	ACTGG	4 cut(s)	270, 445, 856, 1192
BssAI	RCCGGY	3 cut(s)	95, 941, 1267
BssECI	CCNNGG	9 cut(s)	76, 152, 165, 291, 406, 649, 650, 673, 1341
BssMI	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
BssNI	GRCGYC	1 cut(s)	1126
BssT1I	CCWWGG	3 cut(s)	152, 165, 1341
Bst2UI	CCWGG	3 cut(s)	651, 1059, 1230
Bst4CI	ACNGT	5 cut(s)	376, 844, 934, 1066, 1321
BstACI	GRCGYC	1 cut(s)	1126
BstBAI	YACGTR	2 cut(s)	220, 397
BstC8I	GCNNGC	4 cut(s)	525, 529, 782, 981
BstDEI	CTNAG	7 cut(s)	17, 285, 321, 507, 632, 1007, 1158
BstDSI	CCRYGG	2 cut(s)	165, 291
BstEII	GGTNACC	1 cut(s)	937
BstENI	CCTNNNNNAGG	1 cut(s)	1109
BstF5I	GGATG	5 cut(s)	39, 463, 661, 760, 1120
BstH2I	RGCGCY	1 cut(s)	385
BstHHI	GCGC	2 cut(s)	39, 384
BstKTI	GATC	7 cut(s)	83, 87, 137, 164, 226, 589, 973
BstMAI	GTCTC	2 cut(s)	365, 1227
BstMBI	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
BstMCI	CGRYCG	2 cut(s)	12, 87
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 36, 625, 689, 1264
BstNI	CCWGG	3 cut(s)	651, 1059, 1230
BstNSI	RCATGY	1 cut(s)	416
BstPI	GGTNACC	1 cut(s)	937
BstSCI	CCNGG	5 cut(s)	406, 649, 672, 1057, 1228
BstSFI	CTRYAG	3 cut(s)	246, 1150, 1245
BstV1I	GCAGC	3 cut(s)	84, 260, 433
BstV2I	GAAGAC	2 cut(s)	992, 1076
BstXI	CCANNNNNNTGG	1 cut(s)	458
Bsu15I	ATCGAT	1 cut(s)	83
Bsu36I	CCTNAGG	1 cut(s)	321
BsuRI	GGCC	7 cut(s)	170, 290, 296, 317, 493, 855, 1156
BsuTUI	ATCGAT	1 cut(s)	83
BtgI	CCRYGG	2 cut(s)	165, 291
BtsCI	GGATG	5 cut(s)	39, 463, 661, 760, 1120
BtsI	GCAGTG	1 cut(s)	1131
BtsIMutI	CAGTG	3 cut(s)	849, 939, 1131
Cac8I	GCNNGC	4 cut(s)	525, 529, 782, 981
CfoI	GCGC	2 cut(s)	39, 384
Cfr10I	RCCGGY	3 cut(s)	95, 941, 1267
Cfr13I	GGNCC	3 cut(s)	316, 491, 870
ClaI	ATCGAT	1 cut(s)	83
CseI	GACGC	2 cut(s)	247, 1134
Csp6I	GTAC	4 cut(s)	263, 760, 776, 1346
CspAI	ACCGGT	1 cut(s)	941
CviAII	CATG	7 cut(s)	166, 194, 227, 359, 413, 433, 974
CviQI	GTAC	4 cut(s)	263, 760, 776, 1346
DdeI	CTNAG	7 cut(s)	17, 285, 321, 507, 632, 1007, 1158
DpnI	GATC	7 cut(s)	82, 86, 136, 163, 225, 588, 972
DpnII	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
EaeI	YGGCCR	2 cut(s)	168, 853
EciI	GGCGGA	1 cut(s)	106
Ecl136II	GAGCTC	1 cut(s)	619
Eco130I	CCWWGG	3 cut(s)	152, 165, 1341
Eco147I	AGGCCT	1 cut(s)	1156
Eco24I	GRGCYC	1 cut(s)	621
Eco32I	GATATC	1 cut(s)	1030
Eco47I	GGWCC	1 cut(s)	870
Eco53kI	GAGCTC	1 cut(s)	619
Eco72I	CACGTG	1 cut(s)	397
Eco81I	CCTNAGG	1 cut(s)	321
Eco91I	GGTNACC	1 cut(s)	937
EcoICRI	GAGCTC	1 cut(s)	619
EcoNI	CCTNNNNNAGG	1 cut(s)	1109
EcoO109I	RGGNCCY	1 cut(s)	316
EcoO65I	GGTNACC	1 cut(s)	937
EcoRII	CCWGG	3 cut(s)	649, 1057, 1228
EcoRV	GATATC	1 cut(s)	1030
EcoT14I	CCWWGG	3 cut(s)	152, 165, 1341
EcoT38I	GRGCYC	1 cut(s)	621
ErhI	CCWWGG	3 cut(s)	152, 165, 1341
FaeI	CATG	7 cut(s)	169, 197, 230, 362, 416, 436, 977
FalI	AAGNNNNNCTT	2 cut(s)	950, 982
FaqI	GGGAC	1 cut(s)	883
FatI	CATG	7 cut(s)	165, 193, 226, 358, 412, 432, 973
FauI	CCCGC	1 cut(s)	536
FblI	GTMKAC	1 cut(s)	63
Fnu4HI	GCNGC	6 cut(s)	6, 73, 90, 93, 249, 447
FokI	GGATG	5 cut(s)	26, 470, 668, 767, 1127
FriOI	GRGCYC	1 cut(s)	621
Fsp4HI	GCNGC	6 cut(s)	6, 73, 90, 93, 249, 447
FspBI	CTAG	1 cut(s)	27
GlaI	GCGC	2 cut(s)	38, 383
GluI	GCNGC	6 cut(s)	6, 73, 90, 93, 249, 447
GsuI	CTGGAG	1 cut(s)	462
HaeII	RGCGCY	1 cut(s)	385
HaeIII	GGCC	7 cut(s)	170, 290, 296, 317, 493, 855, 1156
HapII	CCGG	5 cut(s)	96, 408, 673, 942, 1268
HgaI	GACGC	2 cut(s)	247, 1134
HhaI	GCGC	2 cut(s)	39, 384
Hin1I	GRCGYC	1 cut(s)	1126
Hin1II	CATG	7 cut(s)	169, 197, 230, 362, 416, 436, 977
Hin6I	GCGC	2 cut(s)	37, 382
HinP1I	GCGC	2 cut(s)	37, 382
HinfI	GANTC	4 cut(s)	113, 746, 898, 1261
HpaII	CCGG	5 cut(s)	96, 408, 673, 942, 1268
HphI	GGTGA	3 cut(s)	896, 949, 998
Hpy166II	GTNNAC	3 cut(s)	64, 400, 1124
Hpy188I	TCNGA	3 cut(s)	20, 112, 125
Hpy188III	TCNNGA	6 cut(s)	485, 590, 767, 799, 958, 1277
Hpy8I	GTNNAC	3 cut(s)	64, 400, 1124
Hpy99I	CGWCG	1 cut(s)	241
HpyAV	CCTTC	5 cut(s)	130, 184, 269, 805, 1112
HpyCH4III	ACNGT	5 cut(s)	376, 844, 934, 1066, 1321
HpyCH4IV	ACGT	4 cut(s)	219, 396, 1101, 1239
HpyCH4V	TGCA	7 cut(s)	72, 248, 350, 392, 416, 1247, 1328
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 36, 625, 689, 1264
HpyF3I	CTNAG	7 cut(s)	17, 285, 321, 507, 632, 1007, 1158
HpySE526I	ACGT	4 cut(s)	219, 396, 1101, 1239
Hsp92I	GRCGYC	1 cut(s)	1126
Hsp92II	CATG	7 cut(s)	169, 197, 230, 362, 416, 436, 977
HspAI	GCGC	2 cut(s)	37, 382
Kzo9I	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
LmnI	GCTCC	6 cut(s)	35, 443, 487, 616, 624, 925
Lsp1109I	GCAGC	3 cut(s)	84, 260, 433
LweI	GCATC	3 cut(s)	48, 565, 792
MaeI	CTAG	1 cut(s)	27
MaeII	ACGT	4 cut(s)	219, 396, 1101, 1239
MaeIII	GTNAC	3 cut(s)	906, 937, 1240
MalI	GATC	7 cut(s)	82, 86, 136, 163, 225, 588, 972
MboI	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
MboII	GAAGA	7 cut(s)	284, 824, 992, 995, 1037, 1081, 1326
MfeI	CAATTG	3 cut(s)	210, 345, 807
MhlI	GDGCHC	2 cut(s)	621, 922
MlsI	TGGCCA	1 cut(s)	170
MluNI	TGGCCA	1 cut(s)	170
MlyI	GAGTC	1 cut(s)	107
MmeI	TCCRAC	3 cut(s)	135, 184, 1057
Mox20I	TGGCCA	1 cut(s)	170
MroXI	GAANNNNTTC	1 cut(s)	516
MscI	TGGCCA	1 cut(s)	170
MseI	TTAA	5 cut(s)	378, 734, 740, 876, 1215
MslI	CAYNNNNRTG	2 cut(s)	198, 915
Msp20I	TGGCCA	1 cut(s)	170
MspI	CCGG	5 cut(s)	96, 408, 673, 942, 1268
MspR9I	CCNGG	5 cut(s)	408, 651, 674, 1059, 1230
MunI	CAATTG	3 cut(s)	210, 345, 807
MvaI	CCWGG	3 cut(s)	651, 1059, 1230
MwoI	GCNNNNNNNGC	5 cut(s)	11, 36, 625, 689, 1264
NciI	CCSGG	2 cut(s)	408, 674
NcoI	CCATGG	1 cut(s)	165
NdeII	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
NlaIII	CATG	7 cut(s)	169, 197, 230, 362, 416, 436, 977
NlaIV	GGNNCC	4 cut(s)	318, 871, 1063, 1272
NmeAIII	GCCGAG	1 cut(s)	101
NmuCI	GTSAC	2 cut(s)	937, 1240
NspI	RCATGY	1 cut(s)	416
PasI	CCCWGGG	1 cut(s)	650
PceI	AGGCCT	1 cut(s)	1156
PdmI	GAANNNNTTC	1 cut(s)	516
PfeI	GAWTC	3 cut(s)	746, 898, 1261
PinAI	ACCGGT	1 cut(s)	941
PkrI	GCNGC	6 cut(s)	7, 74, 91, 94, 250, 448
Ple19I	CGATCG	1 cut(s)	87
PleI	GAGTC	1 cut(s)	107
PmaCI	CACGTG	1 cut(s)	397
PmlI	CACGTG	1 cut(s)	397
PpsI	GAGTC	1 cut(s)	107
Ppu21I	YACGTR	2 cut(s)	220, 397
PshBI	ATTAAT	1 cut(s)	740
Psp124BI	GAGCTC	1 cut(s)	621
Psp6I	CCWGG	3 cut(s)	649, 1057, 1228
PspCI	CACGTG	1 cut(s)	397
PspEI	GGTNACC	1 cut(s)	937
PspGI	CCWGG	3 cut(s)	649, 1057, 1228
PspN4I	GGNNCC	4 cut(s)	318, 871, 1063, 1272
PspPI	GGNCC	3 cut(s)	316, 491, 870
PstI	CTGCAG	2 cut(s)	250, 1249
PvuI	CGATCG	1 cut(s)	87
RsaI	GTAC	4 cut(s)	264, 761, 777, 1347
RsaNI	GTAC	4 cut(s)	263, 760, 776, 1346
RseI	CAYNNNNRTG	2 cut(s)	198, 915
SacI	GAGCTC	1 cut(s)	621
SaqAI	TTAA	5 cut(s)	378, 734, 740, 876, 1215
SatI	GCNGC	6 cut(s)	6, 73, 90, 93, 249, 447
Sau3AI	GATC	7 cut(s)	80, 84, 134, 161, 223, 586, 970
Sau96I	GGNCC	3 cut(s)	316, 491, 870
SchI	GAGTC	1 cut(s)	107
ScrFI	CCNGG	5 cut(s)	408, 651, 674, 1059, 1230
SduI	GDGCHC	2 cut(s)	621, 922
SfaNI	GCATC	3 cut(s)	48, 565, 792
SfcI	CTRYAG	3 cut(s)	246, 1150, 1245
SinI	GGWCC	1 cut(s)	870
SmiMI	CAYNNNNRTG	2 cut(s)	198, 915
SmlI	CTYRAG	1 cut(s)	1300
SmoI	CTYRAG	1 cut(s)	1300
SseBI	AGGCCT	1 cut(s)	1156
SsiI	CCGC	5 cut(s)	8, 90, 93, 117, 529
SspMI	CTAG	1 cut(s)	27
SstI	GAGCTC	1 cut(s)	621
StuI	AGGCCT	1 cut(s)	1156
StyD4I	CCNGG	5 cut(s)	406, 649, 672, 1057, 1228
StyI	CCWWGG	3 cut(s)	152, 165, 1341
TaaI	ACNGT	5 cut(s)	376, 844, 934, 1066, 1321
TaiI	ACGT	4 cut(s)	222, 399, 1104, 1242
TaqI	TCGA	1 cut(s)	83
TaqII	GACCGA	2 cut(s)	147, 986
TatI	WGTACW	1 cut(s)	759
TauI	GCSGC	2 cut(s)	92, 95
TfiI	GAWTC	3 cut(s)	746, 898, 1261
Tru1I	TTAA	5 cut(s)	378, 734, 740, 876, 1215
Tru9I	TTAA	5 cut(s)	378, 734, 740, 876, 1215
TscAI	CASTG	3 cut(s)	856, 939, 1138
TseFI	GTSAC	2 cut(s)	937, 1240
TseI	GCWGC	4 cut(s)	5, 72, 248, 446
Tsp45I	GTSAC	2 cut(s)	937, 1240
TspDTI	ATGAA	2 cut(s)	672, 1131
TspGWI	ACGGA	1 cut(s)	611
TspRI	CASTG	3 cut(s)	856, 939, 1138
VpaK11BI	GGWCC	1 cut(s)	870
VspI	ATTAAT	1 cut(s)	740
XagI	CCTNNNNNAGG	1 cut(s)	1109
XceI	RCATGY	1 cut(s)	416
XmiI	GTMKAC	1 cut(s)	63
XmnI	GAANNNNTTC	1 cut(s)	516
XspI	CTAG	1 cut(s)	27

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.