Rorug06G0221700

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010863
Physical Location & Seq
Forward (+)
35478761 .. 35483938
5178 bp
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UTR
Exon/CDS
Intron
Rorug06G0221700.1

Sequence Viewer

Length: 801 bp
ATGCTAGGCACCCGAAGACAAATTCTCAGCCATTCTCATTACGCACAGAGGTTTTATTCGTCTGAAAGTTATTCAGTTCTTAATTCACCTGGTCTTCAGCACTGGTTCAAGAATTGGCAAGGACTCAGAAAACAGAAACTGACTGCAAGCACTTTTGCTGCGGCTATTGGGTTTTGGAATCGCAGAAGAGTCGGGCTTTGGTTAGAGAAAATTGGAGCAACTGAGCCATTTTCTGGTAACCTGGCTACCTGTTGGAATAATATTAAAGAAGAGGAAGCACTTGAAAGATACAAGTTGATAACGGGAAATTCAGTCTCGTTTCCTGAATTTCAAGTCTATGGAAATGTAAATCCAGGAGATGACTGGCTAGCAGCTTCACCAGATGGGGTGGTTGATAACATGCTGTATGGATTGCCTCGACGAGGAGTGCTGGAGGTTAAATGCCCCTTTTTTGATGGAGATATGAGCAAGGCTTTCCCTTGGTCCCGAATTCCTCTTCACTGTATTCCACAGGCTCAAGGTTTAATGGAAATAGTTGACAGGGAATGGATGGATTTATATGTTTGGACGCCTAATGGAAGTAGCCTATTCAGGTTATACCGAGATGCAGCATACTGGGATATGTTGAAATATGCTCTCTCAGATTTTTGGTGGAATCATGTTCAGCCAGCAAGGGAGTTGTACAGTAAATCTCATGCTATGCATCCTCTCATTGAATCGCTCAGGCCACAACCCAAGCATGAACATTGGCAATATATAGTTTATGAAAGGTATAGAATTTGCAAATTGCAATGTTGCTGA

Protein Analysis

266

Amino Acids

31.22

Weight (kDa)

8.66

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqaJ PF09588 38 - 178 6.4e-16 YqaJ-like viral recombinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02940
fragaria_vesca FvH4_2g24110 FvH4_2g24110 FvH4_2g24110
malus_domestica MD03G1271100.v1.1 MD11G1291700.v1.1
prunus_persica Prupe.8G247100_v2.0.a1
pyrus_communis pycom03g21580 pycom11g25840
rosa_chinensis RchiOBHm_Chr6g0291901
rosa_laevigata RLG00000012080
rosa_multiflora Rmu_sc0002484.1_g000006 Rmu_sc0002484.1_g000007
rosa_roxburghii Rroxscaffold_7G00175640
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG335200 Rh6BG342600 Rh6CG348800 Rh6DG335400
rosa_wichuraiana Rw6G029170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 233
AciI CCGC 1 cut(s) 161
AcsI RAATTY 5 cut(s) 21, 307, 326, 489, 777
AcuI CTGAAG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 569
AfaI GTAC 1 cut(s) 683
AfiI CCNNNNNNNGG 2 cut(s) 233, 422
AgsI TTSAA 5 cut(s) 109, 284, 332, 628, 716
AjnI CCWGG 3 cut(s) 88, 240, 352
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
Alw26I GTCTC 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 3 cut(s) 158, 371, 608
ApoI RAATTY 5 cut(s) 21, 307, 326, 489, 777
AspS9I GGNCC 1 cut(s) 483
AsuHPI GGTGA 2 cut(s) 78, 369
AsuNHI GCTAGC 1 cut(s) 367
AvaII GGWCC 1 cut(s) 483
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 22, 86
BbvI GCAGC 3 cut(s) 145, 383, 620
BccI CCATC 3 cut(s) 377, 449, 544
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 3 cut(s) 90, 242, 354
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 2 cut(s) 5, 368
BisI GCNGC 4 cut(s) 159, 162, 372, 609
BlsI GCNGC 4 cut(s) 160, 163, 373, 610
Bme1390I CCNGG 3 cut(s) 90, 242, 354
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 2 cut(s) 10, 485
BmrFI CCNGG 3 cut(s) 90, 242, 354
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 2 cut(s) 595, 712
BmtI GCTAGC 1 cut(s) 371
BmuI ACTGGG 1 cut(s) 625
BpiI GAAGAC 2 cut(s) 22, 86
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 479
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 422
Bse1I ACTGG 3 cut(s) 107, 368, 620
Bse3DI GCAATG 1 cut(s) 797
BseBI CCWGG 3 cut(s) 90, 242, 354
BseDI CCNNGG 1 cut(s) 479
BseGI GGATG 2 cut(s) 555, 703
BseLI CCNNNNNNNGG 2 cut(s) 233, 422
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 5 cut(s) 40, 139, 213, 654, 736
BseNI ACTGG 3 cut(s) 107, 368, 620
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 3 cut(s) 145, 383, 620
BshFI GGCC 1 cut(s) 727
BshNI GGYRCC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 2 cut(s) 233, 422
BsmAI GTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 469
BsnI GGCC 1 cut(s) 727
Bsp1407I TGTACA 1 cut(s) 681
BspACI CCGC 1 cut(s) 161
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 5 cut(s) 39, 138, 214, 653, 735
BspLI GGNNCC 2 cut(s) 10, 485
BspOI GCTAGC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 3 cut(s) 107, 368, 620
BssECI CCNNGG 1 cut(s) 479
BssNI GRCGYC 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 479
Bst2UI CCWGG 3 cut(s) 90, 242, 354
Bst4CI ACNGT 2 cut(s) 503, 686
Bst6I CTCTTC 3 cut(s) 181, 264, 501
BstACI GRCGYC 1 cut(s) 569
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 3 cut(s) 148, 369, 669
BstDEI CTNAG 5 cut(s) 26, 125, 222, 640, 722
BstEII GGTNACC 1 cut(s) 236
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 555, 703
BstMAI GTCTC 1 cut(s) 319
BstNI CCWGG 3 cut(s) 90, 242, 354
BstNSI RCATGY 1 cut(s) 403
BstPI GGTNACC 1 cut(s) 236
BstSCI CCNGG 3 cut(s) 88, 240, 352
BstV1I GCAGC 3 cut(s) 145, 383, 620
BstV2I GAAGAC 2 cut(s) 22, 86
BsuRI GGCC 1 cut(s) 727
BtsCI GGATG 2 cut(s) 555, 703
BtsIMutI CAGTG 2 cut(s) 100, 499
Cac8I GCNNGC 3 cut(s) 148, 369, 669
CaiI CAGNNNCTG 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 483
CseI GACGC 1 cut(s) 577
CsiI ACCWGGT 1 cut(s) 88
Csp6I GTAC 1 cut(s) 682
CviAII CATG 4 cut(s) 400, 659, 695, 740
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 5 cut(s) 26, 125, 222, 640, 722
Eam1104I CTCTTC 3 cut(s) 181, 264, 501
EarI CTCTTC 3 cut(s) 181, 264, 501
Eco130I CCWWGG 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO65I GGTNACC 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 3 cut(s) 88, 240, 352
EcoT14I CCWWGG 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 479
FaeI CATG 4 cut(s) 403, 662, 698, 743
FaqI GGGAC 1 cut(s) 469
FatI CATG 4 cut(s) 399, 658, 694, 739
Fnu4HI GCNGC 4 cut(s) 159, 162, 372, 609
FokI GGATG 2 cut(s) 562, 690
Fsp4HI GCNGC 4 cut(s) 159, 162, 372, 609
FspBI CTAG 2 cut(s) 5, 368
GluI GCNGC 4 cut(s) 159, 162, 372, 609
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 1 cut(s) 727
HgaI GACGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 4 cut(s) 403, 662, 698, 743
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 5 cut(s) 123, 178, 189, 655, 716
HphI GGTGA 2 cut(s) 78, 369
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 64, 128, 643
Hpy188III TCNNGA 3 cut(s) 109, 323, 486
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 1 cut(s) 423
HpyCH4III ACNGT 2 cut(s) 503, 686
HpyCH4V TGCA 5 cut(s) 146, 608, 703, 783, 790
HpyF3I CTNAG 5 cut(s) 26, 125, 222, 640, 722
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 4 cut(s) 403, 662, 698, 743
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 3 cut(s) 145, 383, 620
LweI GCATC 2 cut(s) 595, 712
MabI ACCWGGT 1 cut(s) 88
MaeI CTAG 2 cut(s) 5, 368
MaeIII GTNAC 1 cut(s) 236
MboII GAAGA 5 cut(s) 27, 86, 198, 281, 488
MluCI AATT 9 cut(s) 21, 82, 112, 210, 307, 326, 489, 777, 785
MlyI GAGTC 2 cut(s) 117, 198
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 7 cut(s) 42, 265, 416, 426, 427, 504, 717
Mph1103I ATGCAT 1 cut(s) 705
MseI TTAA 4 cut(s) 81, 264, 438, 524
MspR9I CCNGG 3 cut(s) 90, 242, 354
MvaI CCWGG 3 cut(s) 90, 242, 354
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 4 cut(s) 403, 662, 698, 743
NlaIV GGNNCC 2 cut(s) 10, 485
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 1 cut(s) 403
PfeI GAWTC 3 cut(s) 178, 655, 716
PflMI CCANNNNNTGG 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 352
PkrI GCNGC 4 cut(s) 160, 163, 373, 610
PleI GAGTC 2 cut(s) 117, 197
PpsI GAGTC 2 cut(s) 117, 197
Psp6I CCWGG 3 cut(s) 88, 240, 352
PspEI GGTNACC 1 cut(s) 236
PspGI CCWGG 3 cut(s) 88, 240, 352
PspN4I GGNNCC 2 cut(s) 10, 485
PspPI GGNCC 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 139
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
SaqAI TTAA 4 cut(s) 81, 264, 438, 524
SatI GCNGC 4 cut(s) 159, 162, 372, 609
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 2 cut(s) 117, 198
ScrFI CCNGG 3 cut(s) 90, 242, 354
SetI ASST 9 cut(s) 53, 91, 243, 251, 376, 438, 523, 596, 773
SexAI ACCWGGT 1 cut(s) 88
SfaNI GCATC 2 cut(s) 595, 712
SinI GGWCC 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 9 cut(s) 21, 82, 112, 210, 307, 326, 489, 777, 785
SsiI CCGC 1 cut(s) 161
SspI AATATT 1 cut(s) 262
SspMI CTAG 2 cut(s) 5, 368
StyD4I CCNGG 3 cut(s) 88, 240, 352
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 2 cut(s) 503, 686
TaqI TCGA 1 cut(s) 418
TasI AATT 9 cut(s) 21, 82, 112, 210, 307, 326, 489, 777, 785
TatI WGTACW 1 cut(s) 681
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 178, 655, 716
Tru1I TTAA 4 cut(s) 81, 264, 438, 524
Tru9I TTAA 4 cut(s) 81, 264, 438, 524
TscAI CASTG 2 cut(s) 107, 506
TseI GCWGC 3 cut(s) 158, 371, 608
TspDTI ATGAA 2 cut(s) 756, 780
TspRI CASTG 2 cut(s) 107, 506
Van91I CCANNNNNTGG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 5 cut(s) 21, 307, 326, 489, 777
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XspI CTAG 2 cut(s) 5, 368
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.