pycom03g21580

oxidoreductase, 2OG-Fe(II) oxygenase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010863
Physical Location & Seq
Forward (+)
21864795 .. 21868642
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21580.2

Sequence Viewer

Length: 933 bp
ATGGCGATGGCGGTTGGGCCAGCTGTGTCGCCGACTGATGGAGCCGCGCCCGTGTTGGGACCCGCAATGATGCCGATGCTGGCTCCGGTGACTATGGTATCTGACCAGCTGGCGAAGGACGCAATTATCGGGTGGTACAGAGGAGAGTTCGCGGCGGCAAACGCAATCATCGATTCCCTCTGCGGCCACCTGACTCAGCTTAGCGGGGGAGGTGGCGCCGAGTACGCCTCTGTTTTCGCAGCCATACACCGGAGAAGGATCAATTGGATCCCGGTGCTCCAGATGCAAAAGTACCACTCCATTGCCGATGTGATGGTGGAGCTCCGAAACGTCGGGCCGAAGAAGGTAAGGGTGGAAAGGTGCGTGGAGAACGGCGACGTTTTGAGAGCAGAAGAAAATTGCAAGAGCGACGAGGCTAAGTCGTGTTTGGAGGCGGACAAAGACAAGGATAGTGACGAGAAGGTGGCGGAGAGTAATGGAATTGTCGCCGCCGATGACGTTGCCGAAGAAGAGGCGTCGTCAGATAGCGAGATTACTGATTCAGGTTCACAAGAAGCACAGCTCAATTCAGTAAACGACGAGATATGCTCTAATCATGACAAGTGTGAGGAGCGTCGTACCCAGATCAAATTGACTAAAGGTTTCTCAGCAAAGGAGTTGGTCAGAGGGCGTATGGTGAATGTTGTGAAAGGGTTAAAGCTATACGAGGACATATTCACTGACACCGAACTCTGCAAATTGACTGACTTCGTAAATGATCTCCACGCGGCTGGACTTAATGGAGAGCTTTCAGGCGAGACCTTCATTTTGTTCAACAAGCAGGTTAAGGGAAACAGGAGAGAGCTGATTCAGTTTGGCGTTCCAATTTTCGGGCAAGATGATCAAGGAGGACACTACAAGGCCGATTCCAGCTCTTCTCGAAGATGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

311

Amino Acids

33.7

Weight (kDa)

4.93

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02940
fragaria_vesca FvH4_2g24110 FvH4_2g24110 FvH4_2g24110
malus_domestica MD03G1271100.v1.1 MD11G1291700.v1.1
prunus_persica Prupe.8G247100_v2.0.a1
pyrus_communis pycom03g21580 pycom11g25840
rosa_chinensis RchiOBHm_Chr6g0291901
rosa_laevigata RLG00000012080
rosa_multiflora Rmu_sc0002484.1_g000006 Rmu_sc0002484.1_g000007
rosa_roxburghii Rroxscaffold_7G00175640
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG335200 Rh6BG342600 Rh6CG348800 Rh6DG335400
rosa_wichuraiana Rw6G029170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 215
AccII CGCG 3 cut(s) 47, 152, 767
AclWI GGATC 3 cut(s) 262, 266, 275
AcoI YGGCCR 1 cut(s) 184
AcyI GRCGYC 2 cut(s) 216, 515
AfaI GTAC 4 cut(s) 137, 224, 293, 619
AfiI CCNNNNNNNGG 4 cut(s) 38, 56, 249, 869
AgsI TTSAA 1 cut(s) 814
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AluBI AGCT 9 cut(s) 23, 109, 199, 322, 562, 700, 787, 844, 912
AluI AGCT 9 cut(s) 23, 109, 199, 322, 562, 700, 787, 844, 912
Alw21I GWGCWC 2 cut(s) 279, 324
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 3 cut(s) 262, 266, 275
AoxI GGCC 4 cut(s) 17, 184, 335, 900
ApeKI GCWGC 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 790, 822
AspLEI GCGC 2 cut(s) 49, 218
AspS9I GGNCC 3 cut(s) 17, 59, 335
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 100, 688
AvaII GGWCC 1 cut(s) 59
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BamHI GGATCC 1 cut(s) 267
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 279, 324
BbvI GCAGC 1 cut(s) 251
BccI CCATC 2 cut(s) 32, 307
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 791
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 7 cut(s) 45, 153, 156, 184, 240, 489, 768
BlpI GCTNAGC 1 cut(s) 200
BlsI GCNGC 7 cut(s) 46, 154, 157, 185, 241, 490, 769
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 3 cut(s) 17, 59, 335
BmiI GGNNCC 6 cut(s) 43, 60, 61, 84, 217, 269
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 60, 66, 273
BplI GAGNNNNNCTC 4 cut(s) 212, 244, 572, 604
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 171
BsaHI GRCGYC 2 cut(s) 216, 515
BsaI GGTCTC 1 cut(s) 791
BsaWI WCCGGW 2 cut(s) 85, 249
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 56, 249, 869
Bse3DI GCAATG 2 cut(s) 72, 300
BseCI ATCGAT 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 38, 56, 249, 869
BseMI GCAATG 2 cut(s) 72, 300
BseMII CTCAG 2 cut(s) 209, 660
BseRI GAGGAG 2 cut(s) 156, 623
BseXI GCAGC 1 cut(s) 251
Bsh1236I CGCG 3 cut(s) 47, 152, 767
BshFI GGCC 4 cut(s) 19, 186, 337, 902
BshNI GGYRCC 1 cut(s) 215
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 279, 324
BsiSI CCGG 3 cut(s) 86, 250, 272
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 4 cut(s) 38, 56, 249, 869
BsmAI GTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 4 cut(s) 19, 186, 337, 902
Bso31I GGTCTC 1 cut(s) 791
Bsp1286I GDGCHC 2 cut(s) 279, 324
Bsp143I GATC 5 cut(s) 258, 267, 624, 757, 880
Bsp1720I GCTNAGC 1 cut(s) 200
BspANI GGCC 4 cut(s) 19, 186, 337, 902
BspCNI CTCAG 2 cut(s) 208, 659
BspDI ATCGAT 1 cut(s) 171
BspFNI CGCG 3 cut(s) 47, 152, 767
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 6 cut(s) 43, 60, 61, 84, 217, 269
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 262, 266, 275
BspQI GCTCTTC 1 cut(s) 919
BspT107I GGYRCC 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 791
BsrDI GCAATG 2 cut(s) 72, 300
BssMI GATC 5 cut(s) 258, 267, 624, 757, 880
BssNI GRCGYC 2 cut(s) 216, 515
Bst6I CTCTTC 2 cut(s) 504, 919
BstACI GRCGYC 2 cut(s) 216, 515
BstC8I GCNNGC 3 cut(s) 21, 81, 111
BstDEI CTNAG 4 cut(s) 195, 200, 417, 646
BstFNI CGCG 3 cut(s) 47, 152, 767
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 2 cut(s) 49, 218
BstKTI GATC 5 cut(s) 261, 270, 627, 760, 883
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 5 cut(s) 258, 267, 624, 757, 880
BstMWI GCNNNNNNNGC 4 cut(s) 119, 161, 224, 283
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 3 cut(s) 47, 152, 767
BstV1I GCAGC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 770
BstYI RGATCY 1 cut(s) 267
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 4 cut(s) 19, 186, 337, 902
BsuTUI ATCGAT 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 717
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 3 cut(s) 21, 81, 111
CciI TCATGA 1 cut(s) 595
CfoI GCGC 2 cut(s) 49, 218
Cfr13I GGNCC 3 cut(s) 17, 59, 335
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 3 cut(s) 128, 504, 602
Csp6I GTAC 4 cut(s) 136, 223, 292, 618
CviAII CATG 1 cut(s) 596
CviQI GTAC 4 cut(s) 136, 223, 292, 618
DdeI CTNAG 4 cut(s) 195, 200, 417, 646
DinI GGCGCC 1 cut(s) 217
DpnI GATC 5 cut(s) 260, 269, 626, 759, 882
DpnII GATC 5 cut(s) 258, 267, 624, 757, 880
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 504, 919
EarI CTCTTC 2 cut(s) 504, 919
EciI GGCGGA 2 cut(s) 449, 482
Ecl136II GAGCTC 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 791
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 324
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
FaeI CATG 1 cut(s) 599
FaiI YATR 7 cut(s) 95, 245, 586, 597, 674, 703, 713
FaqI GGGAC 1 cut(s) 72
FatI CATG 1 cut(s) 595
FauI CCCGC 2 cut(s) 70, 197
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 7 cut(s) 45, 153, 156, 184, 240, 489, 768
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 7 cut(s) 45, 153, 156, 184, 240, 489, 768
GlaI GCGC 2 cut(s) 48, 217
GluI GCNGC 7 cut(s) 45, 153, 156, 184, 240, 489, 768
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 4 cut(s) 19, 186, 337, 902
HapII CCGG 3 cut(s) 86, 250, 272
HgaI GACGC 3 cut(s) 128, 504, 602
HhaI GCGC 2 cut(s) 49, 218
Hin1I GRCGYC 2 cut(s) 216, 515
Hin1II CATG 1 cut(s) 599
Hin6I GCGC 2 cut(s) 47, 216
HinP1I GCGC 2 cut(s) 47, 216
HinfI GANTC 5 cut(s) 173, 193, 539, 847, 905
HpaII CCGG 3 cut(s) 86, 250, 272
HphI GGTGA 2 cut(s) 100, 688
Hpy166II GTNNAC 2 cut(s) 548, 574
Hpy188I TCNGA 4 cut(s) 103, 326, 523, 665
Hpy188III TCNNGA 3 cut(s) 280, 596, 918
Hpy8I GTNNAC 2 cut(s) 548, 574
Hpy99I CGWCG 6 cut(s) 335, 380, 413, 520, 581, 618
HpyAV CCTTC 5 cut(s) 109, 249, 337, 454, 811
HpyCH4IV ACGT 3 cut(s) 330, 378, 498
HpyCH4V TGCA 3 cut(s) 286, 402, 735
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 161, 224, 283
HpyF3I CTNAG 4 cut(s) 195, 200, 417, 646
HpySE526I ACGT 3 cut(s) 330, 378, 498
Hsp92I GRCGYC 2 cut(s) 216, 515
Hsp92II CATG 1 cut(s) 599
HspAI GCGC 2 cut(s) 47, 216
KasI GGCGCC 1 cut(s) 215
KflI GGGWCCC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 5 cut(s) 258, 267, 624, 757, 880
LguI GCTCTTC 1 cut(s) 919
LmnI GCTCC 6 cut(s) 41, 88, 282, 319, 327, 610
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 3 cut(s) 60, 66, 273
MaeII ACGT 3 cut(s) 330, 378, 498
MaeIII GTNAC 2 cut(s) 88, 452
MalI GATC 5 cut(s) 260, 269, 626, 759, 882
MboI GATC 5 cut(s) 258, 267, 624, 757, 880
MboII GAAGA 6 cut(s) 352, 404, 518, 521, 906, 933
MfeI CAATTG 1 cut(s) 262
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 2 cut(s) 279, 324
MluCI AATT 8 cut(s) 123, 262, 397, 480, 565, 629, 737, 864
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 1 cut(s) 187
MseI TTAA 3 cut(s) 695, 777, 825
MspA1I CMGCKG 2 cut(s) 23, 109
MspI CCGG 3 cut(s) 86, 250, 272
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 262
MvnI CGCG 3 cut(s) 47, 152, 767
MwoI GCNNNNNNNGC 4 cut(s) 119, 161, 224, 283
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 1 cut(s) 272
NdeII GATC 5 cut(s) 258, 267, 624, 757, 880
NlaIII CATG 1 cut(s) 599
NlaIV GGNNCC 6 cut(s) 43, 60, 61, 84, 217, 269
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 88, 452
PagI TCATGA 1 cut(s) 595
PciSI GCTCTTC 1 cut(s) 919
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 4 cut(s) 173, 539, 847, 905
PkrI GCNGC 7 cut(s) 46, 154, 157, 185, 241, 490, 769
PleI GAGTC 1 cut(s) 187
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 59
PspN4I GGNNCC 6 cut(s) 43, 60, 61, 84, 217, 269
PspPI GGNCC 3 cut(s) 17, 59, 335
PspPPI RGGWCCY 1 cut(s) 59
PsuI RGATCY 1 cut(s) 267
PvuII CAGCTG 2 cut(s) 23, 109
RsaI GTAC 4 cut(s) 137, 224, 293, 619
RsaNI GTAC 4 cut(s) 136, 223, 292, 618
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 919
SaqAI TTAA 3 cut(s) 695, 777, 825
SatI GCNGC 7 cut(s) 45, 153, 156, 184, 240, 489, 768
Sau3AI GATC 5 cut(s) 258, 267, 624, 757, 880
Sau96I GGNCC 3 cut(s) 17, 59, 335
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 2 cut(s) 279, 324
SfaNI GCATC 3 cut(s) 60, 66, 273
SfoI GGCGCC 1 cut(s) 217
SinI GGWCC 1 cut(s) 59
Sse9I AATT 8 cut(s) 123, 262, 397, 480, 565, 629, 737, 864
SspDI GGCGCC 1 cut(s) 215
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 270
TaiI ACGT 3 cut(s) 333, 381, 501
TaqI TCGA 2 cut(s) 171, 919
TasI AATT 8 cut(s) 123, 262, 397, 480, 565, 629, 737, 864
TauI GCSGC 6 cut(s) 47, 155, 158, 186, 491, 770
TfiI GAWTC 4 cut(s) 173, 539, 847, 905
Tru1I TTAA 3 cut(s) 695, 777, 825
Tru9I TTAA 3 cut(s) 695, 777, 825
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 2 cut(s) 88, 452
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 2 cut(s) 88, 452
TspDTI ATGAA 1 cut(s) 793
TspRI CASTG 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.