AT4G02940

oxidoreductase, 2OG-Fe(II) oxygenase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010863
Physical Location & Seq
Forward (+)
1306313 .. 1311038
4726 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02940.1

Sequence Viewer

Length: 1710 bp
ATGACGATTGCGGCAGCGCCAGCACGCCAGACAGATCGATCAGCCACTGGTTTCAACCCTGCTTACGTCACGACGGCGAAGGCGGTGAGCGTTCCGGTTCAAGTTCCTCCGGCGACTGTGGTTTCCGAAGGATTAGGTAAAGATGCGTTGATCTCATGGTTCAGAGGAGAATTCGCTGCGGCAAACGCGATAATCGACGCGATGTGCTCGCATCTAAGGATAGCTGAAGAAGCGGTATCTGGATCGGAGTACGAAGCTGTATTCGCGGCGATCCATCGCCGTCGTCTGAATTGGATCCCTGTTCTTCAGATGCAGAAGTATCATTCCATCGCTGAAGTAGCGATCGAGCTTCAGAAAGTGGCGGCTAAGAAAGCGGAGGATCTGAAACAGAAGAAAACAGAGGAGGAGGCGGAGGAAGATCTGAAAGAGGTGGTGGCGACGGAGGAAGAGGAAGTGAAGAAAGAGTGTTTTAACGGCGAGAAAGTAACGGAGAATGACGTAAACGGAGATGTAGAAGACGTTGAAGATGATTCACCAACTAGTGATATCACTGATTCAGGTTCTCATCAGGATGTTCACCAAACTGTTGTTGCGGATACTGCACATCAGATTATATGCCATAGCCATGAAGATTGTGATGCACGTTCATGTGAGATCAAACCTATCAAAGGTTTCCAAGCTAAAGAACAAGTCAAAGGCCACACTGTGAATGTTGTGAAAGGACTAAAGCTGTATGAAGAGTTACTTAAAGAAGATGAGATATCTAAATTGTTAGACTTTGTGGCAGAACTACGAGAAGCTGGCATAAACGGGAAGCTTGCAGGTGAGAGCTTTATACTGTTCAACAAACAGATTAAAGGGAACAAGAGAGAGCTGATTCAGCTTGGTGTCCCCATCTTTGGTCATGTCAAGGCGGATGAAAACTCTAACGACACCAACAACTCTGTTAACATCGAGCCAATTCCACCACTTCTTGAGAGTGTCATTGATCACTTTGTCACATGGAGACTCATCCCAGAATACAAGAGACCTAACGGCTGTGTCATCAACTTCTTTGAAGAGGGTGAATACTCACAGCCTTTCCTCAAACCACCTCACTTAGAACAACCAATCTCCACTCTTGTCCTCTCTGAATCAACAATGGCCTATGGACGCATTCTCTCAAGTGACAACGAAGGCAACTTCAGAGGACCTTTGACACTCTCTCTCAAACAAGGATCTTTGTTGGTGATGAGAGGGAACAGTGCAGACATGGCAAGACATGTAATGTGTCCATCACAAAACAAAAGAGTAAGCATCACATTCTTTCGTATTCGACCTGACACATATCATAACCATTCACAACCCAACAGTCCTCGCAACGACGGTGTCATGACAATGTGGCAACCCTACCAAATGACACCAACTCCATTCCTCAATGGTTATGATCATTCAATTGACATGATGCCAAAACTTGGAGTCCTACGTCCTCCAATGGTCATGATGGCACCACCACCGGTTCAACCAATGATATTACCAAGTCCCAATGTGATGGGAACCGGTGGTGGTACCGGTGTTTTCTTACCATGGGCCTCTGTTAATAGCTCAAGAAAACATGTGAAGCATTTGCCTCCACGCGCGCAGAAGAAGCGATTACTTCCGCTTCCTCCTGCTGCTTCTTCTTCTCCAGCTGGAGGATCCACCTCTGAGCCTGTGATCACTGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

569

Amino Acids

62.69

Weight (kDa)

5.75

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02940
fragaria_vesca FvH4_2g24110 FvH4_2g24110 FvH4_2g24110
malus_domestica MD03G1271100.v1.1 MD11G1291700.v1.1
prunus_persica Prupe.8G247100_v2.0.a1
pyrus_communis pycom03g21580 pycom11g25840
rosa_chinensis RchiOBHm_Chr6g0291901
rosa_laevigata RLG00000012080
rosa_multiflora Rmu_sc0002484.1_g000006 Rmu_sc0002484.1_g000007
rosa_roxburghii Rroxscaffold_7G00175640
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG335200 Rh6BG342600 Rh6CG348800 Rh6DG335400
rosa_wichuraiana Rw6G029170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 812
Acc65I GGTACC 1 cut(s) 1547
AccB1I GGYRCC 2 cut(s) 1486, 1547
AccB7I CCANNNNNTGG 1 cut(s) 1454
AccII CGCG 5 cut(s) 188, 200, 266, 1617, 1619
AclWI GGATC 8 cut(s) 250, 265, 289, 302, 387, 1225, 1671, 1684
AcsI RAATTY 1 cut(s) 170
AcuI CTGAAG 5 cut(s) 246, 290, 335, 354, 1168
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 2 cut(s) 251, 1549
AfiI CCNNNNNNNGG 4 cut(s) 668, 899, 1454, 1673
AflIII ACRYGT 2 cut(s) 1261, 1594
AgeI ACCGGT 3 cut(s) 1495, 1538, 1550
AgsI TTSAA 7 cut(s) 55, 101, 524, 844, 1058, 1434, 1502
AhlI ACTAGT 1 cut(s) 539
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 2 cut(s) 1000, 1021
AlwI GGATC 8 cut(s) 250, 265, 289, 302, 387, 1225, 1671, 1684
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 3 cut(s) 697, 1143, 1569
ApeKI GCWGC 3 cut(s) 14, 176, 1652
ApoI RAATTY 1 cut(s) 170
AsiGI ACCGGT 3 cut(s) 1495, 1538, 1550
Asp718I GGTACC 1 cut(s) 1547
AspLEI GCGC 3 cut(s) 19, 1619, 1621
AspS9I GGNCC 2 cut(s) 1190, 1569
AsuHPI GGTGA 6 cut(s) 97, 525, 569, 836, 1076, 1240
AvaII GGWCC 1 cut(s) 1190
BaeI ACNNNNGTAYC 2 cut(s) 1539, 1572
BamHI GGATCC 2 cut(s) 294, 1676
BanI GGYRCC 2 cut(s) 1486, 1547
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 26, 163, 1639
BccI CCATC 6 cut(s) 282, 335, 902, 1282, 1477, 1525
BceAI ACGGC 4 cut(s) 90, 264, 490, 1051
BciVI GTATCC 1 cut(s) 589
BclI TGATCA 3 cut(s) 988, 1426, 1695
BcoDI GTCTC 2 cut(s) 1000, 1021
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 1701
BfoI RGCGCY 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 589
BglII AGATCT 1 cut(s) 418
BisI GCNGC 7 cut(s) 12, 15, 177, 180, 267, 363, 1653
BlsI GCNGC 7 cut(s) 13, 16, 178, 181, 268, 364, 1654
Bme18I GGWCC 1 cut(s) 1190
BmgT120I GGNCC 2 cut(s) 1190, 1569
BmiI GGNNCC 5 cut(s) 296, 1488, 1537, 1549, 1678
BmsI GCATC 6 cut(s) 133, 220, 300, 628, 1305, 1434
BpiI GAAGAC 1 cut(s) 522
BpmI CTGGAG 2 cut(s) 1650, 1692
BpuEI CTTGAG 3 cut(s) 995, 1147, 1570
Bsa29I ATCGAT 1 cut(s) 37
BsaI GGTCTC 1 cut(s) 1021
BsaJI CCNNGG 1 cut(s) 1565
BsaWI WCCGGW 4 cut(s) 94, 1495, 1538, 1550
BsaXI ACNNNNNCTCC 2 cut(s) 1390, 1420
Bsc4I CCNNNNNNNGG 4 cut(s) 668, 899, 1454, 1673
Bse118I RCCGGY 3 cut(s) 1495, 1538, 1550
Bse1I ACTGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 37
BseDI CCNNGG 1 cut(s) 1565
BseGI GGATG 3 cut(s) 577, 922, 1011
BseLI CCNNNNNNNGG 4 cut(s) 668, 899, 1454, 1673
BseMII CTCAG 1 cut(s) 1677
BseNI ACTGG 1 cut(s) 52
BsePI GCGCGC 1 cut(s) 1617
BseRI GAGGAG 3 cut(s) 180, 416, 419
BseXI GCAGC 3 cut(s) 26, 163, 1639
BsgI GTGCAG 2 cut(s) 585, 1266
Bsh1236I CGCG 5 cut(s) 188, 200, 266, 1617, 1619
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 699, 1145, 1571
BshNI GGYRCC 2 cut(s) 1486, 1547
BshTI ACCGGT 3 cut(s) 1495, 1538, 1550
BshVI ATCGAT 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 5 cut(s) 95, 110, 1496, 1539, 1551
BslFI GGGAC 2 cut(s) 875, 1506
BslI CCNNNNNNNGG 4 cut(s) 668, 899, 1454, 1673
BsmAI GTCTC 2 cut(s) 1000, 1021
BsmFI GGGAC 2 cut(s) 875, 1506
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 3 cut(s) 699, 1145, 1571
Bso31I GGTCTC 1 cut(s) 1021
Bsp1286I GDGCHC 1 cut(s) 209
Bsp19I CCATGG 1 cut(s) 1565
BspANI GGCC 3 cut(s) 699, 1145, 1571
BspCNI CTCAG 1 cut(s) 1678
BspDI ATCGAT 1 cut(s) 37
BspFNI CGCG 5 cut(s) 188, 200, 266, 1617, 1619
BspHI TCATGA 2 cut(s) 1371, 1479
BspLI GGNNCC 5 cut(s) 296, 1488, 1537, 1549, 1678
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 8 cut(s) 250, 265, 289, 302, 387, 1225, 1671, 1684
BspT107I GGYRCC 2 cut(s) 1486, 1547
BspTNI GGTCTC 1 cut(s) 1021
BsrFI RCCGGY 3 cut(s) 1495, 1538, 1550
BsrI ACTGG 1 cut(s) 52
BssAI RCCGGY 3 cut(s) 1495, 1538, 1550
BssECI CCNNGG 1 cut(s) 1565
BssHII GCGCGC 1 cut(s) 1617
BssT1I CCWWGG 1 cut(s) 1565
Bst4CI ACNGT 8 cut(s) 118, 586, 706, 840, 1244, 1352, 1367, 1702
Bst6I CTCTTC 3 cut(s) 441, 732, 1053
BstC8I GCNNGC 6 cut(s) 21, 25, 209, 802, 819, 1619
BstDEI CTNAG 4 cut(s) 215, 366, 1099, 1686
BstDSI CCRYGG 1 cut(s) 1565
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 3 cut(s) 577, 922, 1011
BstFNI CGCG 5 cut(s) 188, 200, 266, 1617, 1619
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 3 cut(s) 19, 1619, 1621
BstMAI GTCTC 2 cut(s) 1000, 1021
BstMCI CGRYCG 1 cut(s) 345
BstNSI RCATGY 2 cut(s) 1265, 1598
BstSFI CTRYAG 1 cut(s) 1701
BstUI CGCG 5 cut(s) 188, 200, 266, 1617, 1619
BstV1I GCAGC 3 cut(s) 26, 163, 1639
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 5 cut(s) 294, 379, 418, 1217, 1676
BstXI CCANNNNNNTGG 1 cut(s) 1531
BstYI RGATCY 5 cut(s) 294, 379, 418, 1217, 1676
Bsu15I ATCGAT 1 cut(s) 37
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 3 cut(s) 699, 1145, 1571
BsuTUI ATCGAT 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 1565
BtgZI GCGATG 3 cut(s) 215, 260, 313
BtsCI GGATG 3 cut(s) 577, 922, 1011
BtsIMutI CAGTG 5 cut(s) 45, 549, 702, 1249, 1698
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 6 cut(s) 21, 25, 209, 802, 819, 1619
CaiI CAGNNNCTG 1 cut(s) 47
CciI TCATGA 2 cut(s) 1371, 1479
CfoI GCGC 3 cut(s) 19, 1619, 1621
Cfr10I RCCGGY 3 cut(s) 1495, 1538, 1550
Cfr13I GGNCC 2 cut(s) 1190, 1569
ClaI ATCGAT 1 cut(s) 37
CseI GACGC 2 cut(s) 206, 1161
Csp6I GTAC 2 cut(s) 250, 1548
CspAI ACCGGT 3 cut(s) 1495, 1538, 1550
CviQI GTAC 2 cut(s) 250, 1548
DdeI CTNAG 4 cut(s) 215, 366, 1099, 1686
DraIII CACNNNGTG 1 cut(s) 706
Eam1104I CTCTTC 3 cut(s) 441, 732, 1053
EarI CTCTTC 3 cut(s) 441, 732, 1053
EciI GGCGGA 2 cut(s) 425, 929
Eco130I CCWWGG 1 cut(s) 1565
Eco31I GGTCTC 1 cut(s) 1021
Eco32I GATATC 2 cut(s) 547, 762
Eco47I GGWCC 1 cut(s) 1190
Eco57I CTGAAG 5 cut(s) 246, 290, 335, 354, 1168
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 1190
EcoRI GAATTC 1 cut(s) 170
EcoRV GATATC 2 cut(s) 547, 762
EcoT14I CCWWGG 1 cut(s) 1565
ErhI CCWWGG 1 cut(s) 1565
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 2 cut(s) 875, 1506
FbaI TGATCA 3 cut(s) 988, 1426, 1695
Fnu4HI GCNGC 7 cut(s) 12, 15, 177, 180, 267, 363, 1653
FokI GGATG 3 cut(s) 584, 929, 998
Fsp4HI GCNGC 7 cut(s) 12, 15, 177, 180, 267, 363, 1653
FspBI CTAG 1 cut(s) 540
GlaI GCGC 3 cut(s) 18, 1618, 1620
GluI GCNGC 7 cut(s) 12, 15, 177, 180, 267, 363, 1653
GsuI CTGGAG 2 cut(s) 1650, 1692
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 3 cut(s) 699, 1145, 1571
HapII CCGG 5 cut(s) 95, 110, 1496, 1539, 1551
HgaI GACGC 2 cut(s) 206, 1161
HhaI GCGC 3 cut(s) 19, 1619, 1621
Hin6I GCGC 3 cut(s) 17, 1617, 1619
HinP1I GCGC 3 cut(s) 17, 1617, 1619
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 6 cut(s) 530, 554, 877, 1008, 1133, 1458
HpaI GTTAAC 1 cut(s) 949
HpaII CCGG 5 cut(s) 95, 110, 1496, 1539, 1551
HphI GGTGA 6 cut(s) 97, 525, 569, 836, 1076, 1240
Hpy166II GTNNAC 3 cut(s) 502, 577, 949
Hpy188III TCNNGA 7 cut(s) 70, 240, 569, 974, 1372, 1480, 1587
Hpy8I GTNNAC 3 cut(s) 502, 577, 949
Hpy99I CGWCG 5 cut(s) 76, 200, 285, 442, 1367
HpyAV CCTTC 3 cut(s) 73, 122, 1169
HpyCH4III ACNGT 8 cut(s) 118, 586, 706, 840, 1244, 1352, 1367, 1702
HpyCH4IV ACGT 5 cut(s) 66, 498, 519, 643, 1465
HpyCH4V TGCA 5 cut(s) 313, 602, 641, 821, 1247
HpyF3I CTNAG 4 cut(s) 215, 366, 1099, 1686
HpySE526I ACGT 5 cut(s) 66, 498, 519, 643, 1465
HspAI GCGC 3 cut(s) 17, 1617, 1619
KpnI GGTACC 1 cut(s) 1551
Ksp22I TGATCA 3 cut(s) 988, 1426, 1695
KspAI GTTAAC 1 cut(s) 949
Lsp1109I GCAGC 3 cut(s) 26, 163, 1639
LweI GCATC 6 cut(s) 133, 220, 300, 628, 1305, 1434
MaeI CTAG 1 cut(s) 540
MaeII ACGT 5 cut(s) 66, 498, 519, 643, 1465
MaeIII GTNAC 5 cut(s) 67, 484, 741, 997, 1166
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 5 cut(s) 294, 379, 418, 1217, 1676
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 5 cut(s) 170, 289, 767, 960, 1434
MlyI GAGTC 2 cut(s) 1002, 1467
MseI TTAA 6 cut(s) 471, 747, 855, 948, 1578, 1708
MslI CAYNNNNRTG 4 cut(s) 570, 624, 646, 1376
MspA1I CMGCKG 1 cut(s) 1670
MspI CCGG 5 cut(s) 95, 110, 1496, 1539, 1551
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 1 cut(s) 1155
MvnI CGCG 5 cut(s) 188, 200, 266, 1617, 1619
NcoI CCATGG 1 cut(s) 1565
NlaIV GGNNCC 5 cut(s) 296, 1488, 1537, 1549, 1678
NmuCI GTSAC 3 cut(s) 67, 997, 1166
NspI RCATGY 2 cut(s) 1265, 1598
PagI TCATGA 2 cut(s) 1371, 1479
PaqCI CACCTGC 1 cut(s) 812
PauI GCGCGC 1 cut(s) 1617
PciI ACATGT 2 cut(s) 1261, 1594
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 1155
PfeI GAWTC 4 cut(s) 530, 554, 877, 1133
PflFI GACNNNGTC 1 cut(s) 1367
PflMI CCANNNNNTGG 1 cut(s) 1454
PinAI ACCGGT 3 cut(s) 1495, 1538, 1550
PkrI GCNGC 7 cut(s) 13, 16, 178, 181, 268, 364, 1654
Ple19I CGATCG 1 cut(s) 345
PleI GAGTC 2 cut(s) 1002, 1466
PpsI GAGTC 2 cut(s) 1002, 1466
PpuMI RGGWCCY 1 cut(s) 1190
PscI ACATGT 2 cut(s) 1261, 1594
Psp5II RGGWCCY 1 cut(s) 1190
PspN4I GGNNCC 5 cut(s) 296, 1488, 1537, 1549, 1678
PspPI GGNCC 2 cut(s) 1190, 1569
PspPPI RGGWCCY 1 cut(s) 1190
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 5 cut(s) 294, 379, 418, 1217, 1676
PsyI GACNNNGTC 1 cut(s) 1367
PteI GCGCGC 1 cut(s) 1617
PvuI CGATCG 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 1670
RsaI GTAC 2 cut(s) 251, 1549
RsaNI GTAC 2 cut(s) 250, 1548
RseI CAYNNNNRTG 4 cut(s) 570, 624, 646, 1376
SaqAI TTAA 6 cut(s) 471, 747, 855, 948, 1578, 1708
SatI GCNGC 7 cut(s) 12, 15, 177, 180, 267, 363, 1653
Sau96I GGNCC 2 cut(s) 1190, 1569
SchI GAGTC 2 cut(s) 1002, 1467
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 6 cut(s) 133, 220, 300, 628, 1305, 1434
SfcI CTRYAG 1 cut(s) 1701
SinI GGWCC 1 cut(s) 1190
SmiMI CAYNNNNRTG 4 cut(s) 570, 624, 646, 1376
SmlI CTYRAG 3 cut(s) 974, 1162, 1585
SmoI CTYRAG 3 cut(s) 974, 1162, 1585
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 5 cut(s) 170, 289, 767, 960, 1434
SspMI CTAG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 1565
TaaI ACNGT 8 cut(s) 118, 586, 706, 840, 1244, 1352, 1367, 1702
TaiI ACGT 5 cut(s) 69, 501, 522, 646, 1468
TaqI TCGA 5 cut(s) 37, 195, 345, 954, 1315
TasI AATT 5 cut(s) 170, 289, 767, 960, 1434
TauI GCSGC 4 cut(s) 14, 182, 269, 365
TfiI GAWTC 4 cut(s) 530, 554, 877, 1133
Tru1I TTAA 6 cut(s) 471, 747, 855, 948, 1578, 1708
Tru9I TTAA 6 cut(s) 471, 747, 855, 948, 1578, 1708
TscAI CASTG 5 cut(s) 52, 556, 709, 1249, 1705
TseFI GTSAC 3 cut(s) 67, 997, 1166
TseI GCWGC 3 cut(s) 14, 176, 1652
Tsp45I GTSAC 3 cut(s) 67, 997, 1166
TspDTI ATGAA 4 cut(s) 636, 642, 750, 933
TspGWI ACGGA 3 cut(s) 455, 503, 519
TspRI CASTG 5 cut(s) 52, 556, 709, 1249, 1705
Tth111I GACNNNGTC 1 cut(s) 1367
Van91I CCANNNNNTGG 1 cut(s) 1454
VpaK11BI GGWCC 1 cut(s) 1190
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 1 cut(s) 170
XceI RCATGY 2 cut(s) 1265, 1598
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.