MD03G1271100.v1.1

oxidoreductase, 2OG-Fe(II) oxygenase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010863
Physical Location & Seq
Forward (+)
35463683 .. 35469002
5320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1271100.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGGCGATGGCGGTTGGGCCAGCTGCGTCGCCGACTGATGGAGCCACCCCCATGTTGGGACCCGCAATGATGCCGATGCAGGCTCCGGTGACTGTGGTATCTGACCAGCTGGCGAAGGACGCAATTATCGGGTGGTACAGAGGAGAGTTCGCGGCGGCAAATGCAATCATCGATGCCCTCTGCGGCCACCTGACTCAGCTTAGCGGGGGAGGAGGCGCCGAGTACGCCTCTGTTTTCGCAGCGATACATCGGAGAAGGATCAATTGGATCCCGGTGCTCCAGATGCAGAGGCACCACTCCATTGCCGATGTGATGGTGGAGCTGCGAAACGTCGGGCCGAAGAAGGTAAGGGTGGAAGACTGCGTGGAGAACGGCAACGTTTTGAGAGCAGAAGAAAATTGCAAGAGCGACGAGGTTAAGTCGTGTTTGGACGCCGAGAAAGACAAGGATAGTGACGAGAAGGTGGCGGAGAGTAATGGAATCGTCGCCGCCGATGAGGTTGCCGAAGAAGAGGCGTCCCCGGATAGCGAGATTACTGATTCAGGTTCACAAGAAGCGCAGCTCAATTCAGTAAACGAGGAGATATGCTCTAATCATGACGAGTGTGAGGAGCGTCGTACCCAGATCAAATTGACTAAAGGTTTCTCAGCAAAGGAGTTGGTCAGAGGGCGTATGGTGAATGTCGTGAAAGGGTTAAAGCTATACGAGGACATATTCACTGACACCGAACTCTGCAAATTGACTGACTTCGTAAATGATCTCCACACGGCTGGACTTAATGGAGAGCTCTCAGGCGAGACCTTCATTTTGTTCAACAAGCAGGTTAAGGGAAACAGGAGAGAGCTGATTCAGTTTGGCGTTCCAATTTTCGGGCAGATCAAGGAGGACACTACAAACAATATAGGGCCGATTCCAGCTCTTCTCGAAGATGTGATTGATCACTTTGTACTATGGCAGTTAATTCCGGAGTATAAACGACCGAATGGTTGCATCATCAACTTCTTTGATGAGGGAGAATATTCACAGCCATTCCTGAAACCACCCCATTTGGACCAGCCCATCGCCACTCTTCTCCTCTCTGAATCAGCAATGGCTTTCGGGCGCACCCTTCTAAGCGATAATGATGGGAACTACAAGGGGCCGCTGATGCTATCGCTGAAACAAGGATCTCTTTTAGTCATGAGGGGAAACAGTGCTGACATGTCACGACACGTGATGTGTCCATCTCCAAACAGAAGGGTGAGCATTACATTCTTCAGAGTCCGAGTAGACTCCAACCAGTTCCAGTCGCCCGAAACTCCAACCCACAATGGTGCCATGACTCTATGGCAACCCGGGATGGTGAGCCCATACGCGCTGCCAGATGGAGCAGTTCTCAATGGTTATGAGGCAACGGATGTGATGCCCAAATGGGGTGTCCTTCGTGCTCCAGTAGTCATGATAACCCCCGTTCGTCCCATGGTAATGAGCCCTCGAAAAGTTCCTCAAAGTGGTACCGGGGTTTTCTTGCCCTGGACCAGGGGGTCAAGAAAACCAGCAAAGCATCTTCCACCTCGCGCCCAAAAGGGACGGCTTATGGCACTCCCTTCCCCAGCAGAAACACATGTGGCAGAGTCCACTTCTGAGCCCAGCATCAGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

548

Amino Acids

59.94

Weight (kDa)

5.45

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 330 - 420 2.7e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02940
fragaria_vesca FvH4_2g24110 FvH4_2g24110 FvH4_2g24110
malus_domestica MD03G1271100.v1.1 MD11G1291700.v1.1
prunus_persica Prupe.8G247100_v2.0.a1
pyrus_communis pycom03g21580 pycom11g25840
rosa_chinensis RchiOBHm_Chr6g0291901
rosa_laevigata RLG00000012080
rosa_multiflora Rmu_sc0002484.1_g000006 Rmu_sc0002484.1_g000007
rosa_roxburghii Rroxscaffold_7G00175640
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG335200 Rh6BG342600 Rh6CG348800 Rh6DG335400
rosa_wichuraiana Rw6G029170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1522
Acc36I ACCTGC 1 cut(s) 811
Acc65I GGTACC 1 cut(s) 1493
AccB1I GGYRCC 4 cut(s) 215, 291, 1313, 1493
AccI GTMKAC 1 cut(s) 1269
AccII CGCG 3 cut(s) 152, 1355, 1557
AccIII TCCGGA 1 cut(s) 964
AciI CCGC 9 cut(s) 11, 63, 152, 155, 183, 204, 467, 489, 1142
AclI AACGTT 1 cut(s) 378
AclWI GGATC 4 cut(s) 262, 266, 275, 1174
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 1240
AcvI CACGTG 1 cut(s) 1213
AcyI GRCGYC 3 cut(s) 216, 432, 515
AfaI GTAC 5 cut(s) 137, 224, 619, 948, 1495
AfiI CCNNNNNNNGG 7 cut(s) 38, 55, 56, 869, 1412, 1490, 1518
AflIII ACRYGT 3 cut(s) 1200, 1210, 1603
AgsI TTSAA 1 cut(s) 814
AjnI CCWGG 2 cut(s) 1511, 1517
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AleI CACNNNNGTG 1 cut(s) 1311
AluBI AGCT 9 cut(s) 23, 109, 199, 322, 562, 700, 787, 844, 917
AluI AGCT 9 cut(s) 23, 109, 199, 322, 562, 700, 787, 844, 917
Alw21I GWGCWC 3 cut(s) 279, 789, 1429
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 4 cut(s) 262, 266, 275, 1174
Ama87I CYCGRG 1 cut(s) 1334
Aor13HI TCCGGA 1 cut(s) 964
AoxI GGCC 5 cut(s) 17, 184, 335, 905, 1139
ApeKI GCWGC 5 cut(s) 23, 239, 322, 559, 1357
ArsI GACNNNNNNTTYG 4 cut(s) 790, 822, 1401, 1433
Asp718I GGTACC 1 cut(s) 1493
AspLEI GCGC 5 cut(s) 218, 559, 1104, 1357, 1559
AspS9I GGNCC 7 cut(s) 17, 59, 335, 905, 1051, 1139, 1515
AsuC2I CCSGG 5 cut(s) 272, 521, 1335, 1336, 1498
AsuHPI GGTGA 4 cut(s) 100, 688, 1252, 1354
AvaI CYCGRG 1 cut(s) 1334
AvaII GGWCC 3 cut(s) 59, 1051, 1515
BaeI ACNNNNGTAYC 4 cut(s) 81, 114, 1485, 1518
BamHI GGATCC 1 cut(s) 267
BanI GGYRCC 4 cut(s) 215, 291, 1313, 1493
BanII GRGCYC 4 cut(s) 789, 1349, 1472, 1629
BbrPI CACGTG 1 cut(s) 1213
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 3 cut(s) 279, 789, 1429
BbvI GCAGC 5 cut(s) 10, 251, 309, 571, 1344
BccI CCATC 7 cut(s) 32, 307, 1067, 1118, 1231, 1333, 1358
BceAI ACGGC 3 cut(s) 388, 783, 1586
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 2 cut(s) 1513, 1519
BclI TGATCA 1 cut(s) 937
BcnI CCSGG 5 cut(s) 272, 521, 1335, 1336, 1498
BcoDI GTCTC 1 cut(s) 791
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 811
BlpI GCTNAGC 1 cut(s) 200
Bme1390I CCNGG 7 cut(s) 272, 521, 1335, 1336, 1498, 1513, 1519
Bme18I GGWCC 3 cut(s) 59, 1051, 1515
BmeT110I CYCGRG 1 cut(s) 1334
BmgT120I GGNCC 7 cut(s) 17, 59, 335, 905, 1051, 1139, 1515
BmrFI CCNGG 7 cut(s) 272, 521, 1335, 1336, 1498, 1513, 1519
BmsI GCATC 8 cut(s) 60, 66, 163, 273, 999, 1137, 1392, 1552
BpiI GAAGAC 1 cut(s) 363
BplI GAGNNNNNCTC 6 cut(s) 212, 244, 572, 604, 1359, 1391
BpmI CTGGAG 2 cut(s) 263, 1413
Bpu1102I GCTNAGC 1 cut(s) 200
BpuMI CCSGG 5 cut(s) 272, 521, 1335, 1336, 1498
Bsa29I ATCGAT 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 1213
BsaHI GRCGYC 3 cut(s) 216, 432, 515
BsaI GGTCTC 1 cut(s) 791
BsaJI CCNNGG 6 cut(s) 519, 1334, 1458, 1497, 1511, 1518
BsaWI WCCGGW 2 cut(s) 85, 964
Bsc4I CCNNNNNNNGG 7 cut(s) 38, 55, 56, 869, 1412, 1490, 1518
Bse1I ACTGG 3 cut(s) 1279, 1285, 1430
Bse3DI GCAATG 3 cut(s) 72, 300, 1095
BseAI TCCGGA 1 cut(s) 964
BseBI CCWGG 2 cut(s) 1513, 1519
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 6 cut(s) 519, 1334, 1458, 1497, 1511, 1518
BseGI GGATG 2 cut(s) 1344, 1402
BseLI CCNNNNNNNGG 7 cut(s) 38, 55, 56, 869, 1412, 1490, 1518
BseMI GCAATG 3 cut(s) 72, 300, 1095
BseMII CTCAG 4 cut(s) 209, 660, 804, 1614
BseNI ACTGG 3 cut(s) 1279, 1285, 1430
BseRI GAGGAG 5 cut(s) 156, 225, 593, 623, 1064
BseXI GCAGC 5 cut(s) 10, 251, 309, 571, 1344
BseYI CCCAGC 2 cut(s) 1591, 1628
Bsh1236I CGCG 3 cut(s) 152, 1355, 1557
Bsh1285I CGRYCG 1 cut(s) 980
BshFI GGCC 5 cut(s) 19, 186, 337, 907, 1141
BshNI GGYRCC 4 cut(s) 215, 291, 1313, 1493
BshVI ATCGAT 1 cut(s) 171
BsiEI CGRYCG 1 cut(s) 980
BsiHKAI GWGCWC 3 cut(s) 279, 789, 1429
BsiHKCI CYCGRG 1 cut(s) 1334
BsiSI CCGG 6 cut(s) 86, 272, 521, 965, 1335, 1497
BslFI GGGAC 4 cut(s) 72, 502, 1440, 1581
BslI CCNNNNNNNGG 7 cut(s) 38, 55, 56, 869, 1412, 1490, 1518
BsmAI GTCTC 1 cut(s) 791
BsmFI GGGAC 4 cut(s) 72, 502, 1440, 1581
BsnI GGCC 5 cut(s) 19, 186, 337, 907, 1141
Bso31I GGTCTC 1 cut(s) 791
BsoBI CYCGRG 1 cut(s) 1334
Bsp1286I GDGCHC 6 cut(s) 279, 789, 1349, 1429, 1472, 1629
Bsp13I TCCGGA 1 cut(s) 964
Bsp143I GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
Bsp1720I GCTNAGC 1 cut(s) 200
Bsp19I CCATGG 1 cut(s) 1458
BspACI CCGC 9 cut(s) 11, 63, 152, 155, 183, 204, 467, 489, 1142
BspANI GGCC 5 cut(s) 19, 186, 337, 907, 1141
BspCNI CTCAG 4 cut(s) 208, 659, 803, 1615
BspDI ATCGAT 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 964
BspFNI CGCG 3 cut(s) 152, 1355, 1557
BspHI TCATGA 3 cut(s) 595, 1179, 1437
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 4 cut(s) 262, 266, 275, 1174
BspQI GCTCTTC 1 cut(s) 924
BspT107I GGYRCC 4 cut(s) 215, 291, 1313, 1493
BspTNI GGTCTC 1 cut(s) 791
BsrDI GCAATG 3 cut(s) 72, 300, 1095
BsrI ACTGG 3 cut(s) 1279, 1285, 1430
BssECI CCNNGG 6 cut(s) 519, 1334, 1458, 1497, 1511, 1518
BssMI GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
BssNI GRCGYC 3 cut(s) 216, 432, 515
BssT1I CCWWGG 1 cut(s) 1458
Bst2UI CCWGG 2 cut(s) 1513, 1519
Bst4CI ACNGT 2 cut(s) 94, 1193
Bst6I CTCTTC 3 cut(s) 504, 924, 1074
BstACI GRCGYC 3 cut(s) 216, 432, 515
BstBAI YACGTR 1 cut(s) 1213
BstC8I GCNNGC 3 cut(s) 21, 81, 111
BstDEI CTNAG 6 cut(s) 195, 200, 646, 790, 1112, 1623
BstDSI CCRYGG 1 cut(s) 1458
BstENI CCTNNNNNAGG 1 cut(s) 1516
BstF5I GGATG 2 cut(s) 1344, 1402
BstFNI CGCG 3 cut(s) 152, 1355, 1557
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 5 cut(s) 218, 559, 1104, 1357, 1559
BstKTI GATC 7 cut(s) 261, 270, 627, 760, 879, 940, 1169
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
BstMCI CGRYCG 1 cut(s) 980
BstMWI GCNNNNNNNGC 5 cut(s) 119, 161, 224, 283, 1147
BstNI CCWGG 2 cut(s) 1513, 1519
BstNSI RCATGY 2 cut(s) 1204, 1607
BstSCI CCNGG 7 cut(s) 270, 519, 1333, 1334, 1496, 1511, 1517
BstUI CGCG 3 cut(s) 152, 1355, 1557
BstV1I GCAGC 5 cut(s) 10, 251, 309, 571, 1344
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 2 cut(s) 267, 1166
BstXI CCANNNNNNTGG 1 cut(s) 770
BstYI RGATCY 2 cut(s) 267, 1166
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 5 cut(s) 19, 186, 337, 907, 1141
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 1458
BtgZI GCGATG 2 cut(s) 20, 1045
BtsCI GGATG 2 cut(s) 1344, 1402
BtsIMutI CAGTG 3 cut(s) 717, 1198, 1642
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 3 cut(s) 21, 81, 111
CciI TCATGA 3 cut(s) 595, 1179, 1437
CfoI GCGC 5 cut(s) 218, 559, 1104, 1357, 1559
Cfr13I GGNCC 7 cut(s) 17, 59, 335, 905, 1051, 1139, 1515
Cfr9I CCCGGG 1 cut(s) 1334
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 5 cut(s) 15, 128, 440, 504, 602
Csp6I GTAC 5 cut(s) 136, 223, 618, 947, 1494
CspCI CAANNNNNGTGG 2 cut(s) 1029, 1064
CviAII CATG 8 cut(s) 52, 596, 1180, 1201, 1318, 1438, 1459, 1604
CviQI GTAC 5 cut(s) 136, 223, 618, 947, 1494
DdeI CTNAG 6 cut(s) 195, 200, 646, 790, 1112, 1623
DinI GGCGCC 1 cut(s) 217
DpnI GATC 7 cut(s) 260, 269, 626, 759, 878, 939, 1168
DpnII GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
DrdI GACNNNNNNGTC 1 cut(s) 1522
DseDI GACNNNNNNGTC 1 cut(s) 1522
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 3 cut(s) 504, 924, 1074
EarI CTCTTC 3 cut(s) 504, 924, 1074
EciI GGCGGA 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 787
Eco130I CCWWGG 1 cut(s) 1458
Eco24I GRGCYC 4 cut(s) 789, 1349, 1472, 1629
Eco31I GGTCTC 1 cut(s) 791
Eco47I GGWCC 3 cut(s) 59, 1051, 1515
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 1240
Eco72I CACGTG 1 cut(s) 1213
Eco88I CYCGRG 1 cut(s) 1334
EcoICRI GAGCTC 1 cut(s) 787
EcoNI CCTNNNNNAGG 1 cut(s) 1516
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 1511, 1517
EcoT14I CCWWGG 1 cut(s) 1458
EcoT38I GRGCYC 4 cut(s) 789, 1349, 1472, 1629
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 1458
FaeI CATG 8 cut(s) 55, 599, 1183, 1204, 1321, 1441, 1462, 1607
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FaqI GGGAC 4 cut(s) 72, 502, 1440, 1581
FatI CATG 8 cut(s) 51, 595, 1179, 1200, 1317, 1437, 1458, 1603
FauI CCCGC 2 cut(s) 70, 197
FbaI TGATCA 1 cut(s) 937
FblI GTMKAC 1 cut(s) 1269
FokI GGATG 2 cut(s) 1351, 1409
FriOI GRGCYC 4 cut(s) 789, 1349, 1472, 1629
GlaI GCGC 5 cut(s) 217, 558, 1103, 1356, 1558
GsaI CCCAGC 2 cut(s) 1595, 1632
GsuI CTGGAG 2 cut(s) 263, 1413
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 5 cut(s) 19, 186, 337, 907, 1141
HapII CCGG 6 cut(s) 86, 272, 521, 965, 1335, 1497
HgaI GACGC 5 cut(s) 15, 128, 440, 504, 602
HhaI GCGC 5 cut(s) 218, 559, 1104, 1357, 1559
Hin1I GRCGYC 3 cut(s) 216, 432, 515
Hin1II CATG 8 cut(s) 55, 599, 1183, 1204, 1321, 1441, 1462, 1607
Hin6I GCGC 5 cut(s) 216, 557, 1102, 1355, 1557
HinP1I GCGC 5 cut(s) 216, 557, 1102, 1355, 1557
HpaII CCGG 6 cut(s) 86, 272, 521, 965, 1335, 1497
HphI GGTGA 4 cut(s) 100, 688, 1252, 1354
Hpy166II GTNNAC 4 cut(s) 548, 574, 1270, 1617
Hpy188I TCNGA 7 cut(s) 103, 252, 665, 1081, 1259, 1265, 1624
Hpy8I GTNNAC 4 cut(s) 548, 574, 1270, 1617
Hpy99I CGWCG 5 cut(s) 31, 335, 413, 488, 618
HpyAV CCTTC 9 cut(s) 109, 249, 337, 454, 811, 1118, 1230, 1430, 1596
HpyCH4III ACNGT 2 cut(s) 94, 1193
HpyCH4IV ACGT 3 cut(s) 330, 378, 1212
HpyCH4V TGCA 6 cut(s) 79, 164, 286, 402, 735, 990
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 161, 224, 283, 1147
HpyF3I CTNAG 6 cut(s) 195, 200, 646, 790, 1112, 1623
HpySE526I ACGT 3 cut(s) 330, 378, 1212
Hsp92I GRCGYC 3 cut(s) 216, 432, 515
Hsp92II CATG 8 cut(s) 55, 599, 1183, 1204, 1321, 1441, 1462, 1607
HspAI GCGC 5 cut(s) 216, 557, 1102, 1355, 1557
KasI GGCGCC 1 cut(s) 215
KflI GGGWCCC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 964
KpnI GGTACC 1 cut(s) 1497
Ksp22I TGATCA 1 cut(s) 937
Kzo9I GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
LguI GCTCTTC 1 cut(s) 924
LmnI GCTCC 7 cut(s) 41, 88, 282, 319, 610, 1367, 1432
Lsp1109I GCAGC 5 cut(s) 10, 251, 309, 571, 1344
LweI GCATC 8 cut(s) 60, 66, 163, 273, 999, 1137, 1392, 1552
MaeII ACGT 3 cut(s) 330, 378, 1212
MaeIII GTNAC 3 cut(s) 88, 452, 1203
MalI GATC 7 cut(s) 260, 269, 626, 759, 878, 939, 1168
MboI GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
MfeI CAATTG 1 cut(s) 262
MflI RGATCY 2 cut(s) 267, 1166
MhlI GDGCHC 6 cut(s) 279, 789, 1349, 1429, 1472, 1629
MluCI AATT 8 cut(s) 123, 262, 397, 565, 629, 737, 864, 960
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 5 cut(s) 187, 1265, 1269, 1315, 1622
MmeI TCCRAC 2 cut(s) 1299, 1325
MroI TCCGGA 1 cut(s) 964
MseI TTAA 5 cut(s) 417, 695, 777, 825, 959
MslI CAYNNNNRTG 3 cut(s) 50, 1311, 1463
MspA1I CMGCKG 3 cut(s) 23, 109, 1144
MspI CCGG 6 cut(s) 86, 272, 521, 965, 1335, 1497
MspR9I CCNGG 7 cut(s) 272, 521, 1335, 1336, 1498, 1513, 1519
MunI CAATTG 1 cut(s) 262
MvaI CCWGG 2 cut(s) 1513, 1519
MvnI CGCG 3 cut(s) 152, 1355, 1557
MwoI GCNNNNNNNGC 5 cut(s) 119, 161, 224, 283, 1147
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 5 cut(s) 272, 521, 1335, 1336, 1498
NcoI CCATGG 1 cut(s) 1458
NdeII GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
NlaIII CATG 8 cut(s) 55, 599, 1183, 1204, 1321, 1441, 1462, 1607
NmeAIII GCCGAG 2 cut(s) 244, 460
NmuCI GTSAC 3 cut(s) 88, 452, 1203
NspI RCATGY 2 cut(s) 1204, 1607
OliI CACNNNNGTG 1 cut(s) 1311
PagI TCATGA 3 cut(s) 595, 1179, 1437
PciI ACATGT 2 cut(s) 1200, 1603
PciSI GCTCTTC 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PfeI GAWTC 5 cut(s) 480, 539, 847, 910, 1082
PleI GAGTC 5 cut(s) 187, 1265, 1268, 1315, 1621
PluTI GGCGCC 1 cut(s) 219
PmaCI CACGTG 1 cut(s) 1213
PmlI CACGTG 1 cut(s) 1213
PpsI GAGTC 5 cut(s) 187, 1265, 1268, 1315, 1621
Ppu21I YACGTR 1 cut(s) 1213
PpuMI RGGWCCY 1 cut(s) 59
PscI ACATGT 2 cut(s) 1200, 1603
Psp124BI GAGCTC 1 cut(s) 789
Psp1406I AACGTT 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 1511, 1517
PspCI CACGTG 1 cut(s) 1213
PspFI CCCAGC 2 cut(s) 1591, 1628
PspGI CCWGG 2 cut(s) 1511, 1517
PspPI GGNCC 7 cut(s) 17, 59, 335, 905, 1051, 1139, 1515
PspPPI RGGWCCY 1 cut(s) 59
PsuI RGATCY 2 cut(s) 267, 1166
PvuII CAGCTG 2 cut(s) 23, 109
RsaI GTAC 5 cut(s) 137, 224, 619, 948, 1495
RsaNI GTAC 5 cut(s) 136, 223, 618, 947, 1494
RseI CAYNNNNRTG 3 cut(s) 50, 1311, 1463
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 924
SaqAI TTAA 5 cut(s) 417, 695, 777, 825, 959
Sau3AI GATC 7 cut(s) 258, 267, 624, 757, 876, 937, 1166
Sau96I GGNCC 7 cut(s) 17, 59, 335, 905, 1051, 1139, 1515
SchI GAGTC 5 cut(s) 187, 1265, 1269, 1315, 1622
ScrFI CCNGG 7 cut(s) 272, 521, 1335, 1336, 1498, 1513, 1519
SduI GDGCHC 6 cut(s) 279, 789, 1349, 1429, 1472, 1629
SfaNI GCATC 8 cut(s) 60, 66, 163, 273, 999, 1137, 1392, 1552
SfoI GGCGCC 1 cut(s) 217
SinI GGWCC 3 cut(s) 59, 1051, 1515
SmaI CCCGGG 1 cut(s) 1336
SmiMI CAYNNNNRTG 3 cut(s) 50, 1311, 1463
Sse9I AATT 8 cut(s) 123, 262, 397, 565, 629, 737, 864, 960
SsiI CCGC 9 cut(s) 11, 63, 152, 155, 183, 204, 467, 489, 1142
SspDI GGCGCC 1 cut(s) 215
SspI AATATT 1 cut(s) 1019
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 7 cut(s) 270, 519, 1333, 1334, 1496, 1511, 1517
StyI CCWWGG 1 cut(s) 1458
TaaI ACNGT 2 cut(s) 94, 1193
TaiI ACGT 3 cut(s) 333, 381, 1215
TaqI TCGA 3 cut(s) 171, 924, 1474
TaqII GACCGA 1 cut(s) 994
TasI AATT 8 cut(s) 123, 262, 397, 565, 629, 737, 864, 960
TatI WGTACW 1 cut(s) 946
TauI GCSGC 5 cut(s) 155, 158, 186, 491, 1144
TfiI GAWTC 5 cut(s) 480, 539, 847, 910, 1082
Tru1I TTAA 5 cut(s) 417, 695, 777, 825, 959
Tru9I TTAA 5 cut(s) 417, 695, 777, 825, 959
TscAI CASTG 3 cut(s) 724, 1198, 1642
TseFI GTSAC 3 cut(s) 88, 452, 1203
TseI GCWGC 5 cut(s) 23, 239, 322, 559, 1357
Tsp45I GTSAC 3 cut(s) 88, 452, 1203
TspDTI ATGAA 1 cut(s) 793
TspGWI ACGGA 1 cut(s) 1409
TspMI CCCGGG 1 cut(s) 1334
TspRI CASTG 3 cut(s) 724, 1198, 1642
VpaK11BI GGWCC 3 cut(s) 59, 1051, 1515
XagI CCTNNNNNAGG 1 cut(s) 1516
XceI RCATGY 2 cut(s) 1204, 1607
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmaI CCCGGG 1 cut(s) 1334
XmiI GTMKAC 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.