FvH4_2g24110

oxidoreductase, 2OG-Fe(II) oxygenase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010863
Physical Location & Seq
Forward (+)
19702086 .. 19706888
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24110.t1

Sequence Viewer

Length: 1617 bp
ATGGCGATGGCGGTTGGGCCAGCTGCGTTGCCGCCGGCCGATGGAGGCTGCAGTCCTCCTCCAGCGATGGGACCCGCAATGATGCCAGTCCCCGACTCGGTGGCCAAGGACGCGATAATCGGTTGGTACCGCGGCGAGTTCGCGGCTGCCAACGCGATCATCGACGCTCTCTGCGGCCACCTGACTCAGCTCAGCGGAGGAGGCGCCGAGTACAGCGCCGTGTTCGCCGCGATACACCGGAGGAGGATGAATTGGATCCCGGTGCTCCAGATGCAGAAGTACCACTCCATAGCTGACGTGATGGTGGAGCTCCGAAACCTCGGGGCCAAGATGGCGGCGGAGCGGGATAGCCACCACGAGAACGGCGACGTTTTTGAGGCGGAGGACAATTGCAAGAGCAGCGAAGGGGCGAAGTCGTGTGTGGAGGACGAGAAGGTGGATGAAAGTAATGCAAATGAGGTCGCCGCCGATGAGGTCACTGAAGAAGACGGTTCGCCGGAAAGTGATATCACTGACTCAGGTTCTCAAGAAGTGCTCAATTCATTGATCACTGAGATATGTTCTAACCATGATGACTGTGAGGCACGTCGTGCCCAGATCAAGTTGACAAAAGGTTTCTCAGCAAAGGAGTCAGTCAAAGGGCATATGGTTAATGTTGTGAAAGGCTTAAAGCTATATGAGGAAATATTTACCGACTCTGAGCTCTCTAAATTGACTGACTTTGCAAATGAACTCCGCACGGCTGGTCTGAATGGAGAGCTCTCAGGCGAGACCTTTATTTTGTTCAACAAGCAAATTAAGGGAAACAGGAGAGAGCTAATTCAGTTTGGAATTCCAATATTTGGACAGGTCAAAGAGGAAGCTGCAAGCAACATTGGGCCAATTCCGGCTCTTCTCCATGATGTGATCGATCACCTTGTACTGTGGAACCTAATCCCAGAGTACAAACGACCTAATGGTTGCATCATCAACTTCTTTGAGGAGGGAGAATATTCGCAGCCATTCCTGAAACCACCACATTTGGATCAGCCAATCTCGACTCTTCTCCTCTCTGAATCCACAATGGCTTACGGGCGCACCCTTGTGACTGATTCTGATGGGAACTACAAGGGGCCGCTGACCCTCTCTTTGAAGGAAGGGTCACTCCTAGTTATGAGGGGAAACAGTGCTGACATGGCAAGGCACGTCATGTGTCCATCTCCGAACAAGAGGATCAGCATCACATTCTTTAGAGTCCGAGTAGACTCTAACCAATATCAGTCGTCACCACAAAGTCCAACACAGAATGGTGCCATGACTGTATGGCAGCCTGGAGTTATGAGCCCATATGCAGCTCCAGATGGAGCACCTAATGGCTACGAGGCAATGGATGTAATGCCAAAATGGGGTGTCCTCCGTGCTCCAGTGGTCATGTTAGCCCCAGTTCGTCCTATGGTATTGAGCCCTCGAAAACTTCCTCAAAGTGGGACTGGAGTTTTCTTGCCGTGGACTGTGGGATCAAGAAAACCAGCAAAACATCTTCCACCTCGCGCCCAAAAAGGGCGGCTTATGGCACTCCCTTCCCCAGCCGTGACACATGTAGTGGAGTCCACTTCTGAGCCAGGCATCACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

539

Amino Acids

58.37

Weight (kDa)

5.42

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 321 - 411 5.1e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02940
fragaria_vesca FvH4_2g24110 FvH4_2g24110 FvH4_2g24110
malus_domestica MD03G1271100.v1.1 MD11G1291700.v1.1
prunus_persica Prupe.8G247100_v2.0.a1
pyrus_communis pycom03g21580 pycom11g25840
rosa_chinensis RchiOBHm_Chr6g0291901
rosa_laevigata RLG00000012080
rosa_multiflora Rmu_sc0002484.1_g000006 Rmu_sc0002484.1_g000007
rosa_roxburghii Rroxscaffold_7G00175640
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG335200 Rh6BG342600 Rh6CG348800 Rh6DG335400
rosa_wichuraiana Rw6G029170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 3 cut(s) 126, 203, 1289
AccB7I CCANNNNNTGG 1 cut(s) 842
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
AclWI GGATC 5 cut(s) 250, 263, 1032, 1220, 1504
AcoI YGGCCR 3 cut(s) 36, 102, 175
AcsI RAATTY 1 cut(s) 831
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 590
AfaI GTAC 5 cut(s) 128, 212, 281, 921, 944
AfiI CCNNNNNNNGG 7 cut(s) 41, 68, 97, 842, 1385, 1463, 1539
AflIII ACRYGT 1 cut(s) 1576
AgsI TTSAA 2 cut(s) 787, 1132
AjiI CACGTC 3 cut(s) 298, 587, 1186
AjnI CCWGG 2 cut(s) 1309, 1600
AleI CACNNNNGTG 1 cut(s) 1082
Alw21I GWGCWC 7 cut(s) 267, 312, 537, 705, 762, 1348, 1402
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 5 cut(s) 250, 263, 1032, 1220, 1504
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 7 cut(s) 17, 36, 102, 175, 324, 878, 1112
ApeKI GCWGC 8 cut(s) 23, 48, 146, 399, 863, 997, 1306, 1331
ApoI RAATTY 1 cut(s) 831
ArsI GACNNNNNNTTYG 4 cut(s) 763, 795, 1111, 1143
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 4 cut(s) 206, 218, 1077, 1532
AspS9I GGNCC 5 cut(s) 17, 71, 324, 878, 1112
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 905, 1257
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 595
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 255
BanI GGYRCC 3 cut(s) 126, 203, 1289
BanII GRGCYC 5 cut(s) 312, 705, 762, 1325, 1445
BauI CACGAG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 7 cut(s) 267, 312, 537, 705, 762, 1348, 1402
BbvI GCAGC 8 cut(s) 10, 35, 133, 411, 850, 1009, 1318, 1343
BccI CCATC 7 cut(s) 35, 61, 295, 325, 1091, 1204, 1334
BceAI ACGGC 5 cut(s) 203, 379, 756, 1468, 1553
BciT130I CCWGG 2 cut(s) 1311, 1602
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 1 cut(s) 1148
BfmI CTRYAG 1 cut(s) 49
BfoI RGCGCY 2 cut(s) 207, 219
BglI GCCNNNNNGGC 1 cut(s) 332
BlpI GCTNAGC 1 cut(s) 191
Bme1390I CCNGG 3 cut(s) 260, 1311, 1602
Bme18I GGWCC 1 cut(s) 71
BmeT110I CYCGRG 1 cut(s) 320
BmgBI CACGTC 3 cut(s) 298, 587, 1186
BmgT120I GGNCC 5 cut(s) 17, 71, 324, 878, 1112
BmiI GGNNCC 9 cut(s) 72, 73, 128, 205, 257, 325, 929, 1113, 1291
BmrFI CCNGG 3 cut(s) 260, 1311, 1602
BmrI ACTGGG 1 cut(s) 1415
BmsI GCATC 4 cut(s) 72, 261, 972, 1227
BmuI ACTGGG 1 cut(s) 1415
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 6 cut(s) 45, 251, 1320, 1332, 1386, 1491
Bpu1102I GCTNAGC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 909
BsaBI GATNNNNATC 1 cut(s) 1217
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 105, 130, 319, 1484
BsaWI WCCGGW 1 cut(s) 237
Bsc4I CCNNNNNNNGG 7 cut(s) 41, 68, 97, 842, 1385, 1463, 1539
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 4 cut(s) 86, 1403, 1421, 1474
Bse3DI GCAATG 2 cut(s) 84, 1371
Bse8I GATNNNNATC 1 cut(s) 1217
BseBI CCWGG 2 cut(s) 1311, 1602
BseCI ATCGAT 1 cut(s) 909
BseDI CCNNGG 4 cut(s) 105, 130, 319, 1484
BseGI GGATG 3 cut(s) 252, 445, 1375
BseJI GATNNNNATC 1 cut(s) 1217
BseLI CCNNNNNNNGG 7 cut(s) 41, 68, 97, 842, 1385, 1463, 1539
BseMI GCAATG 2 cut(s) 84, 1371
BseMII CTCAG 8 cut(s) 200, 205, 531, 543, 633, 690, 777, 1587
BseNI ACTGG 4 cut(s) 86, 1403, 1421, 1474
BseRI GAGGAG 5 cut(s) 48, 213, 256, 995, 1037
BseSI GKGCMC 1 cut(s) 595
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 8 cut(s) 10, 35, 133, 411, 850, 1009, 1318, 1343
BseYI CCCAGC 1 cut(s) 1564
Bsh1236I CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 7 cut(s) 19, 38, 104, 177, 326, 880, 1114
BshNI GGYRCC 3 cut(s) 126, 203, 1289
BshVI ATCGAT 1 cut(s) 909
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 7 cut(s) 267, 312, 537, 705, 762, 1348, 1402
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 5 cut(s) 35, 238, 260, 497, 887
BslFI GGGAC 3 cut(s) 74, 84, 1480
BslI CCNNNNNNNGG 7 cut(s) 41, 68, 97, 842, 1385, 1463, 1539
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 3 cut(s) 74, 84, 1480
BsnI GGCC 7 cut(s) 19, 38, 104, 177, 326, 880, 1114
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 320
Bsp143I GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
Bsp1720I GCTNAGC 1 cut(s) 191
BspANI GGCC 7 cut(s) 19, 38, 104, 177, 326, 880, 1114
BspCNI CTCAG 8 cut(s) 199, 204, 530, 544, 632, 691, 776, 1588
BspDI ATCGAT 1 cut(s) 909
BspFNI CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
BspLI GGNNCC 9 cut(s) 72, 73, 128, 205, 257, 325, 929, 1113, 1291
BspMAI CTGCAG 1 cut(s) 53
BspPI GGATC 5 cut(s) 250, 263, 1032, 1220, 1504
BspQI GCTCTTC 1 cut(s) 897
BspT107I GGYRCC 3 cut(s) 126, 203, 1289
BspTNI GGTCTC 1 cut(s) 764
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 84, 1371
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 4 cut(s) 86, 1403, 1421, 1474
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 4 cut(s) 105, 130, 319, 1484
BssMI GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 105
Bst2BI CACGAG 1 cut(s) 356
Bst2UI CCWGG 2 cut(s) 1311, 1602
Bst4CI ACNGT 7 cut(s) 491, 578, 924, 1166, 1300, 1492, 1612
Bst6I CTCTTC 2 cut(s) 897, 1047
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 3 cut(s) 21, 36, 868
BstDEI CTNAG 8 cut(s) 186, 191, 517, 552, 619, 699, 763, 1596
BstDSI CCRYGG 2 cut(s) 130, 1484
BstF5I GGATG 3 cut(s) 252, 445, 1375
BstFNI CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
BstH2I RGCGCY 2 cut(s) 207, 219
BstHHI GCGC 4 cut(s) 206, 218, 1077, 1532
BstKTI GATC 9 cut(s) 159, 258, 549, 600, 909, 913, 1027, 1215, 1499
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 9 cut(s) 110, 152, 201, 224, 271, 332, 399, 590, 1175
BstNI CCWGG 2 cut(s) 1311, 1602
BstNSI RCATGY 1 cut(s) 1580
BstSCI CCNGG 3 cut(s) 258, 1309, 1600
BstSFI CTRYAG 1 cut(s) 49
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
BstV1I GCAGC 8 cut(s) 10, 35, 133, 411, 850, 1009, 1318, 1343
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 909
BsuRI GGCC 7 cut(s) 19, 38, 104, 177, 326, 880, 1114
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 2 cut(s) 130, 1484
BtgZI GCGATG 2 cut(s) 20, 80
BtrI CACGTC 3 cut(s) 298, 587, 1186
BtsCI GGATG 3 cut(s) 252, 445, 1375
BtsIMutI CAGTG 6 cut(s) 477, 510, 549, 1171, 1410, 1608
Cac8I GCNNGC 3 cut(s) 21, 36, 868
CfoI GCGC 4 cut(s) 206, 218, 1077, 1532
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 5 cut(s) 17, 71, 324, 878, 1112
Cfr42I CCGCGG 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 909
CseI GACGC 2 cut(s) 119, 173
Csp6I GTAC 5 cut(s) 127, 211, 280, 920, 943
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 7 cut(s) 569, 899, 1174, 1189, 1294, 1411, 1577
CviQI GTAC 5 cut(s) 127, 211, 280, 920, 943
DdeI CTNAG 8 cut(s) 186, 191, 517, 552, 619, 699, 763, 1596
DinI GGCGCC 1 cut(s) 205
DpnI GATC 9 cut(s) 158, 257, 548, 599, 908, 912, 1026, 1214, 1498
DpnII GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
DraIII CACNNNGTG 1 cut(s) 590
EaeI YGGCCR 3 cut(s) 36, 102, 175
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 897, 1047
EarI CTCTTC 2 cut(s) 897, 1047
EciI GGCGGA 2 cut(s) 353, 395
Ecl136II GAGCTC 3 cut(s) 310, 703, 760
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 5 cut(s) 312, 705, 762, 1325, 1445
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 71
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 3 cut(s) 310, 703, 760
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 3 cut(s) 310, 703, 760
EcoO109I RGGNCCY 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 831
EcoRII CCWGG 2 cut(s) 1309, 1600
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 5 cut(s) 312, 705, 762, 1325, 1445
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 7 cut(s) 572, 902, 1177, 1192, 1297, 1414, 1580
FalI AAGNNNNNCTT 2 cut(s) 1530, 1562
FaqI GGGAC 3 cut(s) 74, 84, 1480
FatI CATG 7 cut(s) 568, 898, 1173, 1188, 1293, 1410, 1576
FauI CCCGC 2 cut(s) 82, 336
FauNDI CATATG 2 cut(s) 645, 1327
FbaI TGATCA 1 cut(s) 546
FblI GTMKAC 1 cut(s) 1242
FokI GGATG 3 cut(s) 259, 452, 1382
FriOI GRGCYC 5 cut(s) 312, 705, 762, 1325, 1445
FspBI CTAG 1 cut(s) 1148
GlaI GCGC 4 cut(s) 205, 217, 1076, 1531
GsaI CCCAGC 1 cut(s) 1568
GsuI CTGGAG 6 cut(s) 45, 251, 1320, 1332, 1386, 1491
HaeII RGCGCY 2 cut(s) 207, 219
HaeIII GGCC 7 cut(s) 19, 38, 104, 177, 326, 880, 1114
HapII CCGG 5 cut(s) 35, 238, 260, 497, 887
HgaI GACGC 2 cut(s) 119, 173
HhaI GCGC 4 cut(s) 206, 218, 1077, 1532
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 572, 902, 1177, 1192, 1297, 1414, 1580
Hin6I GCGC 4 cut(s) 204, 216, 1075, 1530
HinP1I GCGC 4 cut(s) 204, 216, 1075, 1530
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HpaII CCGG 5 cut(s) 35, 238, 260, 497, 887
HphI GGTGA 2 cut(s) 905, 1257
Hpy166II GTNNAC 4 cut(s) 606, 1243, 1488, 1590
Hpy188I TCNGA 8 cut(s) 314, 700, 750, 1054, 1096, 1203, 1238, 1597
Hpy188III TCNNGA 6 cut(s) 268, 527, 1006, 1036, 1337, 1500
Hpy8I GTNNAC 4 cut(s) 606, 1243, 1488, 1590
Hpy99I CGWCG 3 cut(s) 167, 371, 591
HpyAV CCTTC 5 cut(s) 398, 427, 1126, 1130, 1569
HpyCH4III ACNGT 7 cut(s) 491, 578, 924, 1166, 1300, 1492, 1612
HpyCH4IV ACGT 4 cut(s) 297, 369, 586, 1185
HpyCH4V TGCA 8 cut(s) 51, 274, 393, 452, 725, 866, 963, 1331
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 152, 201, 224, 271, 332, 399, 590, 1175
HpyF3I CTNAG 8 cut(s) 186, 191, 517, 552, 619, 699, 763, 1596
HpySE526I ACGT 4 cut(s) 297, 369, 586, 1185
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 572, 902, 1177, 1192, 1297, 1414, 1580
HspAI GCGC 4 cut(s) 204, 216, 1075, 1530
KasI GGCGCC 1 cut(s) 203
KflI GGGWCCC 1 cut(s) 71
KpnI GGTACC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 546
KspI CCGCGG 1 cut(s) 133
Kzo9I GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 7 cut(s) 270, 307, 315, 340, 1339, 1343, 1405
Lsp1109I GCAGC 8 cut(s) 10, 35, 133, 411, 850, 1009, 1318, 1343
LweI GCATC 4 cut(s) 72, 261, 972, 1227
MaeI CTAG 1 cut(s) 1148
MaeII ACGT 4 cut(s) 297, 369, 586, 1185
MaeIII GTNAC 5 cut(s) 475, 1084, 1140, 1263, 1570
MalI GATC 9 cut(s) 158, 257, 548, 599, 908, 912, 1026, 1214, 1498
MbiI CCGCTC 1 cut(s) 343
MboI GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
MboII GAAGA 5 cut(s) 494, 497, 884, 1034, 1511
MfeI CAATTG 1 cut(s) 388
MflI RGATCY 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 8 cut(s) 250, 388, 538, 710, 795, 819, 831, 882
MluNI TGGCCA 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 9 cut(s) 89, 178, 509, 638, 689, 1033, 1238, 1242, 1595
MmeI TCCRAC 1 cut(s) 1301
Mox20I TGGCCA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 34
MscI TGGCCA 1 cut(s) 104
MseI TTAA 4 cut(s) 651, 668, 798, 1615
MslI CAYNNNNRTG 1 cut(s) 1082
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 4 cut(s) 23, 132, 195, 1117
MspI CCGG 5 cut(s) 35, 238, 260, 497, 887
MspR9I CCNGG 3 cut(s) 260, 1311, 1602
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 2 cut(s) 1311, 1602
MvnI CGCG 6 cut(s) 113, 132, 143, 155, 230, 1530
MwoI GCNNNNNNNGC 9 cut(s) 110, 152, 201, 224, 271, 332, 399, 590, 1175
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 1 cut(s) 260
NdeI CATATG 2 cut(s) 645, 1327
NdeII GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 7 cut(s) 572, 902, 1177, 1192, 1297, 1414, 1580
NlaIV GGNNCC 9 cut(s) 72, 73, 128, 205, 257, 325, 929, 1113, 1291
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 5 cut(s) 475, 1084, 1140, 1263, 1570
NspI RCATGY 1 cut(s) 1580
OliI CACNNNNGTG 1 cut(s) 1082
PciI ACATGT 1 cut(s) 1576
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 363
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 1055, 1091
PflMI CCANNNNNTGG 1 cut(s) 842
PleI GAGTC 9 cut(s) 89, 178, 509, 637, 689, 1033, 1238, 1241, 1594
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 9 cut(s) 89, 178, 509, 637, 689, 1033, 1238, 1241, 1594
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 1 cut(s) 1576
Psp124BI GAGCTC 3 cut(s) 312, 705, 762
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 1309, 1600
PspFI CCCAGC 1 cut(s) 1564
PspGI CCWGG 2 cut(s) 1309, 1600
PspN4I GGNNCC 9 cut(s) 72, 73, 128, 205, 257, 325, 929, 1113, 1291
PspPI GGNCC 5 cut(s) 17, 71, 324, 878, 1112
PspPPI RGGWCCY 1 cut(s) 71
PstI CTGCAG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 5 cut(s) 128, 212, 281, 921, 944
RsaNI GTAC 5 cut(s) 127, 211, 280, 920, 943
RseI CAYNNNNRTG 1 cut(s) 1082
SacI GAGCTC 3 cut(s) 312, 705, 762
SacII CCGCGG 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 4 cut(s) 651, 668, 798, 1615
Sau3AI GATC 9 cut(s) 156, 255, 546, 597, 906, 910, 1024, 1212, 1496
Sau96I GGNCC 5 cut(s) 17, 71, 324, 878, 1112
SchI GAGTC 9 cut(s) 89, 178, 509, 638, 689, 1033, 1238, 1242, 1595
ScrFI CCNGG 3 cut(s) 260, 1311, 1602
SfaNI GCATC 4 cut(s) 72, 261, 972, 1227
SfcI CTRYAG 1 cut(s) 49
SfoI GGCGCC 1 cut(s) 205
Sfr303I CCGCGG 1 cut(s) 133
SgrBI CCGCGG 1 cut(s) 133
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 8 cut(s) 250, 388, 538, 710, 795, 819, 831, 882
SspDI GGCGCC 1 cut(s) 203
SspI AATATT 3 cut(s) 687, 840, 992
SspMI CTAG 1 cut(s) 1148
SstI GAGCTC 3 cut(s) 312, 705, 762
StyD4I CCNGG 3 cut(s) 258, 1309, 1600
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 7 cut(s) 491, 578, 924, 1166, 1300, 1492, 1612
TaiI ACGT 4 cut(s) 300, 372, 589, 1188
TaqI TCGA 4 cut(s) 162, 909, 1037, 1447
TasI AATT 8 cut(s) 250, 388, 538, 710, 795, 819, 831, 882
TatI WGTACW 3 cut(s) 210, 919, 942
TauI GCSGC 9 cut(s) 34, 135, 146, 177, 230, 338, 467, 1117, 1546
TfiI GAWTC 2 cut(s) 1055, 1091
Tru1I TTAA 4 cut(s) 651, 668, 798, 1615
Tru9I TTAA 4 cut(s) 651, 668, 798, 1615
TscAI CASTG 6 cut(s) 484, 517, 556, 1171, 1410, 1615
TseFI GTSAC 5 cut(s) 475, 1084, 1140, 1263, 1570
TseI GCWGC 8 cut(s) 23, 48, 146, 399, 863, 997, 1306, 1331
Tsp45I GTSAC 5 cut(s) 475, 1084, 1140, 1263, 1570
TspDTI ATGAA 4 cut(s) 263, 456, 531, 744
TspGWI ACGGA 1 cut(s) 1385
TspRI CASTG 6 cut(s) 484, 517, 556, 1171, 1410, 1615
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 1 cut(s) 831
XceI RCATGY 1 cut(s) 1580
XmiI GTMKAC 1 cut(s) 1242
XspI CTAG 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.