Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012731

Physical Location & Seq

Genomic Coordinates Reverse (-)

35837332 .. 35839737

Gene Structure 2406 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1275900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 738 bp

ATGGCTGGATACAAAGCCGACGATGACTACGACTACCTCTTCAAGGTGGTCTTGATTGGGGACTCCGGTGTGGGCAAGTCCAATCTGCTCTCCAGGTTCACTAAGAACGAGTTCAACCTGGAGTCCAAATCCACCATTGGAGTTGAATTCGCCACCAGGACCTTAACCGTTGACGGCAAAGTCATCAAGGCTCAGATTTGGGACACTGCTGGCCAAGAAAGGTTATGTGCTTTTGTGAAGTTTAAATTGTGGGGTTTGGTGAAAGTTTGGATAGATTTGGGTGAAGATTTAGGTTCTCTCTTGTTGTATCGCGCCATCACTAGTGCGTACTATCGCGGGGCTGTGGGTGCACTCCTTGTGTACGATGTCACTCGCCATGCGACATTTGAGAATGTTGACAGATGGCTGAAAGAATTGAGGAACCACACCGATTCCAACATTGTTGTGATGCTTGTTGGGAACAAATCAGATCTGCGCCACCTTGTAGCTGTCTCAACTGAAGATGGGAAGTCCTATGCCGAGAAGGAATCACTGTACTTCATGGAAACATCTGCACTGGAAGCAAAGAATGTGGAAAACTCCTTCGCTGAAGTTCTGACTCAAATATACCATGTTGTGAGCAAGAAGGCAGTTGAAGGGGGAGAAAACGGAACTTCATCAGTCCCAGCACAAGGAGAGAAAATAAATATAAAGGATGATGTGTCCGCTTTGAAGAGAGTCGGGTGCTGCTCAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0006972 GO:0008104 GO:0008150 GO:0009628 GO:0009651 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017076 GO:0019001 GO:0031090 GO:0031410 GO:0031982 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0034613 GO:0035639 GO:0036094 GO:0042538 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060627 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097159 GO:0097367 GO:0097708 GO:0098588 GO:0098805 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

246

Amino Acids

27.13

Weight (kDa)

5.88

Isoelectric Point (pI)

22.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ras	PF00071	15 - 77	1.5e-23	Ras family
Roc	PF08477	15 - 77	1.3e-18	Ras of Complex, Roc, domain of DAPkinase
Roc	PF08477	102 - 157	1.3e-09	Ras of Complex, Roc, domain of DAPkinase
Ras	PF00071	103 - 203	9.8e-32	Ras family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16920
fragaria_vesca	FvH4_3g18190
malus_domestica	MD00G1077000.v1.1 MD03G1275900.v1.1 MD11G1264700.v1.1
prunus_persica	Prupe.4G161600_v2.0.a1
pyrus_communis	pycom03g19300 pycom11g23300
rosa_chinensis	RchiOBHm_Chr5g0030721
rosa_laevigata	RLG00000033247
rosa_multiflora	Rmu_sc0003238.1_g000034
rosa_rugosa	Rorug05G0120400
rosa_samantha	Rh5AG211900 Rh5BG211000 Rh5CG234700 Rh5DG216400
rosa_wichuraiana	Rw0G000930 Rw5G019450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AccII	CGCG	2 cut(s)	312, 336
AciI	CCGC	2 cut(s)	336, 705
AcoI	YGGCCR	1 cut(s)	211
AcsI	RAATTY	1 cut(s)	146
AcuI	CTGAAG	2 cut(s)	519, 609
AfaI	GTAC	3 cut(s)	329, 362, 536
AfiI	CCNNNNNNNGG	2 cut(s)	43, 671
AgsI	TTSAA	5 cut(s)	43, 115, 146, 635, 712
AhlI	ACTAGT	1 cut(s)	320
AjnI	CCWGG	3 cut(s)	92, 117, 155
AluBI	AGCT	2 cut(s)	488, 735
AluI	AGCT	2 cut(s)	488, 735
Alw21I	GWGCWC	1 cut(s)	352
Alw26I	GTCTC	1 cut(s)	496
Alw44I	GTGCAC	1 cut(s)	348
AoxI	GGCC	1 cut(s)	211
ApaLI	GTGCAC	1 cut(s)	348
ApeKI	GCWGC	1 cut(s)	726
ApoI	RAATTY	1 cut(s)	146
Asp700I	GAANNNNTTC	1 cut(s)	110
AspLEI	GCGC	2 cut(s)	314, 477
AspS9I	GGNCC	1 cut(s)	159
AsuHPI	GGTGA	2 cut(s)	271, 293
AvaII	GGWCC	1 cut(s)	159
BaeGI	GKGCMC	1 cut(s)	352
BalI	TGGCCA	1 cut(s)	213
Bbv12I	GWGCWC	1 cut(s)	352
BbvI	GCAGC	1 cut(s)	713
BccI	CCATC	3 cut(s)	323, 396, 497
BceAI	ACGGC	1 cut(s)	190
BciT130I	CCWGG	3 cut(s)	94, 119, 157
BcoDI	GTCTC	1 cut(s)	496
BcuI	ACTAGT	1 cut(s)	320
BfaI	CTAG	2 cut(s)	321, 736
BglII	AGATCT	1 cut(s)	469
BisI	GCNGC	1 cut(s)	727
BlsI	GCNGC	1 cut(s)	728
Bme1390I	CCNGG	3 cut(s)	94, 119, 157
Bme18I	GGWCC	1 cut(s)	159
BmgT120I	GGNCC	1 cut(s)	159
BmiI	GGNNCC	1 cut(s)	422
BmrFI	CCNGG	3 cut(s)	94, 119, 157
BmsI	GCATC	1 cut(s)	438
BpmI	CTGGAG	2 cut(s)	76, 140
BpuEI	CTTGAG	1 cut(s)	715
BsaWI	WCCGGW	1 cut(s)	65
Bsc4I	CCNNNNNNNGG	2 cut(s)	43, 671
Bse1I	ACTGG	1 cut(s)	561
BseBI	CCWGG	3 cut(s)	94, 119, 157
BseGI	GGATG	1 cut(s)	700
BseLI	CCNNNNNNNGG	2 cut(s)	43, 671
BseMII	CTCAG	1 cut(s)	206
BseNI	ACTGG	1 cut(s)	561
BseSI	GKGCMC	1 cut(s)	352
BseXI	GCAGC	1 cut(s)	713
BseYI	CCCAGC	1 cut(s)	664
BsgI	GTGCAG	1 cut(s)	537
Bsh1236I	CGCG	2 cut(s)	312, 336
BshFI	GGCC	1 cut(s)	213
BsiHKAI	GWGCWC	1 cut(s)	352
BsiSI	CCGG	1 cut(s)	66
BslFI	GGGAC	3 cut(s)	74, 215, 647
BslI	CCNNNNNNNGG	2 cut(s)	43, 671
BsmAI	GTCTC	1 cut(s)	496
BsmFI	GGGAC	3 cut(s)	74, 215, 647
BsnI	GGCC	1 cut(s)	213
Bsp1286I	GDGCHC	1 cut(s)	352
Bsp143I	GATC	1 cut(s)	469
BspACI	CCGC	2 cut(s)	336, 705
BspANI	GGCC	1 cut(s)	213
BspCNI	CTCAG	1 cut(s)	205
BspFNI	CGCG	2 cut(s)	312, 336
BspLI	GGNNCC	1 cut(s)	422
BsrI	ACTGG	1 cut(s)	561
BssMI	GATC	1 cut(s)	469
Bst2UI	CCWGG	3 cut(s)	94, 119, 157
Bst4CI	ACNGT	2 cut(s)	169, 534
Bst6I	CTCTTC	2 cut(s)	44, 707
BstC8I	GCNNGC	1 cut(s)	211
BstDEI	CTNAG	2 cut(s)	102, 192
BstENI	CCTNNNNNAGG	1 cut(s)	41
BstF5I	GGATG	1 cut(s)	700
BstFNI	CGCG	2 cut(s)	312, 336
BstHHI	GCGC	2 cut(s)	314, 477
BstKTI	GATC	1 cut(s)	472
BstMAI	GTCTC	1 cut(s)	496
BstMBI	GATC	1 cut(s)	469
BstMWI	GCNNNNNNNGC	3 cut(s)	347, 560, 732
BstNI	CCWGG	3 cut(s)	94, 119, 157
BstSCI	CCNGG	3 cut(s)	92, 117, 155
BstSLI	GKGCMC	1 cut(s)	352
BstUI	CGCG	2 cut(s)	312, 336
BstV1I	GCAGC	1 cut(s)	713
BstX2I	RGATCY	1 cut(s)	469
BstYI	RGATCY	1 cut(s)	469
BsuRI	GGCC	1 cut(s)	213
BtsCI	GGATG	1 cut(s)	700
BtsI	GCAGTG	1 cut(s)	204
BtsIMutI	CAGTG	3 cut(s)	204, 530, 554
Cac8I	GCNNGC	1 cut(s)	211
CfoI	GCGC	2 cut(s)	314, 477
Cfr13I	GGNCC	1 cut(s)	159
Csp6I	GTAC	3 cut(s)	328, 361, 535
CspCI	CAANNNNNGTGG	2 cut(s)	552, 587
CviAII	CATG	3 cut(s)	377, 541, 611
CviJI	RGCY	8 cut(s)	5, 17, 191, 213, 341, 406, 488, 735
CviKI_1	RGCY	8 cut(s)	5, 17, 191, 213, 341, 406, 488, 735
CviQI	GTAC	3 cut(s)	328, 361, 535
DdeI	CTNAG	2 cut(s)	102, 192
DpnI	GATC	1 cut(s)	471
DpnII	GATC	1 cut(s)	469
DraI	TTTAAA	1 cut(s)	244
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	1 cut(s)	211
Eam1104I	CTCTTC	2 cut(s)	44, 707
EarI	CTCTTC	2 cut(s)	44, 707
Eco47I	GGWCC	1 cut(s)	159
Eco57I	CTGAAG	2 cut(s)	519, 609
EcoNI	CCTNNNNNAGG	1 cut(s)	41
EcoO109I	RGGNCCY	1 cut(s)	159
EcoRI	GAATTC	1 cut(s)	146
EcoRII	CCWGG	3 cut(s)	92, 117, 155
FaeI	CATG	3 cut(s)	380, 544, 614
FaiI	YATR	7 cut(s)	226, 378, 516, 542, 607, 612, 689
FalI	AAGNNNNNCTT	2 cut(s)	35, 67
FaqI	GGGAC	3 cut(s)	74, 215, 647
FatI	CATG	3 cut(s)	376, 540, 610
FauI	CCCGC	1 cut(s)	329
Fnu4HI	GCNGC	1 cut(s)	727
FokI	GGATG	1 cut(s)	707
Fsp4HI	GCNGC	1 cut(s)	727
FspBI	CTAG	2 cut(s)	321, 736
GlaI	GCGC	2 cut(s)	313, 476
GluI	GCNGC	1 cut(s)	727
GsaI	CCCAGC	1 cut(s)	668
GsuI	CTGGAG	2 cut(s)	76, 140
HaeIII	GGCC	1 cut(s)	213
HapII	CCGG	1 cut(s)	66
HhaI	GCGC	2 cut(s)	314, 477
Hin1II	CATG	3 cut(s)	380, 544, 614
Hin6I	GCGC	2 cut(s)	312, 475
HinP1I	GCGC	2 cut(s)	312, 475
HincII	GTYRAC	2 cut(s)	172, 397
HindII	GTYRAC	2 cut(s)	172, 397
HinfI	GANTC	6 cut(s)	62, 122, 431, 527, 598, 717
HpaII	CCGG	1 cut(s)	66
HphI	GGTGA	2 cut(s)	271, 293
Hpy166II	GTNNAC	5 cut(s)	99, 172, 350, 361, 397
Hpy188I	TCNGA	3 cut(s)	195, 469, 597
Hpy188III	TCNNGA	1 cut(s)	52
Hpy8I	GTNNAC	5 cut(s)	99, 172, 350, 361, 397
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	4 cut(s)	517, 592, 619, 629
HpyCH4III	ACNGT	2 cut(s)	169, 534
HpyCH4V	TGCA	2 cut(s)	350, 554
HpyF10VI	GCNNNNNNNGC	3 cut(s)	347, 560, 732
HpyF3I	CTNAG	2 cut(s)	102, 192
Hsp92II	CATG	3 cut(s)	380, 544, 614
HspAI	GCGC	2 cut(s)	312, 475
Kzo9I	GATC	1 cut(s)	469
Lsp1109I	GCAGC	1 cut(s)	713
LweI	GCATC	1 cut(s)	438
MaeI	CTAG	2 cut(s)	321, 736
MaeIII	GTNAC	1 cut(s)	367
MalI	GATC	1 cut(s)	471
MboI	GATC	1 cut(s)	469
MboII	GAAGA	4 cut(s)	31, 296, 512, 724
MflI	RGATCY	1 cut(s)	469
MhlI	GDGCHC	1 cut(s)	352
MlsI	TGGCCA	1 cut(s)	213
MluCI	AATT	3 cut(s)	146, 245, 413
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	4 cut(s)	56, 131, 592, 726
MmeI	TCCRAC	1 cut(s)	459
MnlI	CCTC	2 cut(s)	47, 411
Mox20I	TGGCCA	1 cut(s)	213
MroXI	GAANNNNTTC	1 cut(s)	110
MscI	TGGCCA	1 cut(s)	213
MseI	TTAA	2 cut(s)	164, 243
MslI	CAYNNNNRTG	1 cut(s)	443
Msp20I	TGGCCA	1 cut(s)	213
MspI	CCGG	1 cut(s)	66
MspR9I	CCNGG	3 cut(s)	94, 119, 157
MvaI	CCWGG	3 cut(s)	94, 119, 157
MvnI	CGCG	2 cut(s)	312, 336
MwoI	GCNNNNNNNGC	3 cut(s)	347, 560, 732
NdeII	GATC	1 cut(s)	469
NlaIII	CATG	3 cut(s)	380, 544, 614
NlaIV	GGNNCC	1 cut(s)	422
NmeAIII	GCCGAG	1 cut(s)	544
NmuCI	GTSAC	1 cut(s)	367
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PdmI	GAANNNNTTC	1 cut(s)	110
PfeI	GAWTC	2 cut(s)	431, 527
PkrI	GCNGC	1 cut(s)	728
PleI	GAGTC	4 cut(s)	56, 130, 592, 725
PpsI	GAGTC	4 cut(s)	56, 130, 592, 725
PpuMI	RGGWCCY	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	159
Psp6I	CCWGG	3 cut(s)	92, 117, 155
PspFI	CCCAGC	1 cut(s)	664
PspGI	CCWGG	3 cut(s)	92, 117, 155
PspN4I	GGNNCC	1 cut(s)	422
PspPI	GGNCC	1 cut(s)	159
PspPPI	RGGWCCY	1 cut(s)	159
PsuI	RGATCY	1 cut(s)	469
RsaI	GTAC	3 cut(s)	329, 362, 536
RsaNI	GTAC	3 cut(s)	328, 361, 535
RseI	CAYNNNNRTG	1 cut(s)	443
SaqAI	TTAA	2 cut(s)	164, 243
SatI	GCNGC	1 cut(s)	727
Sau3AI	GATC	1 cut(s)	469
Sau96I	GGNCC	1 cut(s)	159
SchI	GAGTC	4 cut(s)	56, 131, 592, 726
ScrFI	CCNGG	3 cut(s)	94, 119, 157
SduI	GDGCHC	1 cut(s)	352
SfaNI	GCATC	1 cut(s)	438
SinI	GGWCC	1 cut(s)	159
SmiMI	CAYNNNNRTG	1 cut(s)	443
SmlI	CTYRAG	1 cut(s)	730
SmoI	CTYRAG	1 cut(s)	730
SpeI	ACTAGT	1 cut(s)	320
Sse9I	AATT	3 cut(s)	146, 245, 413
SsiI	CCGC	2 cut(s)	336, 705
SspMI	CTAG	2 cut(s)	321, 736
StyD4I	CCNGG	3 cut(s)	92, 117, 155
TaaI	ACNGT	2 cut(s)	169, 534
TasI	AATT	3 cut(s)	146, 245, 413
TatI	WGTACW	1 cut(s)	534
TfiI	GAWTC	2 cut(s)	431, 527
Tru1I	TTAA	2 cut(s)	164, 243
Tru9I	TTAA	2 cut(s)	164, 243
TscAI	CASTG	3 cut(s)	211, 537, 561
TseFI	GTSAC	1 cut(s)	367
TseI	GCWGC	1 cut(s)	726
Tsp45I	GTSAC	1 cut(s)	367
TspDTI	ATGAA	2 cut(s)	529, 645
TspGWI	ACGGA	1 cut(s)	663
TspRI	CASTG	3 cut(s)	211, 537, 561
VneI	GTGCAC	1 cut(s)	348
VpaK11BI	GGWCC	1 cut(s)	159
XagI	CCTNNNNNAGG	1 cut(s)	41
XapI	RAATTY	1 cut(s)	146
XmnI	GAANNNNTTC	1 cut(s)	110
XspI	CTAG	2 cut(s)	321, 736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.