Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0012731

Physical Location & Seq

Genomic Coordinates Reverse (-)

37968529 .. 37971379

Gene Structure 2851 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1264700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 660 bp

ATGGCTGGATACAGAGCCGACGATGACTATGACTACCTCTTCAAGTTGGTCTTGATTGGGGACTCCGGCGTGGGGAAGTCGAATCTGCTCTCTAGGTTCACCAAGAACGAGTTCAACCTCGAGTCCAAATCCACCATTGGCGTTGAGTTTGCCACCAGGACCTTGACCGTTGACGGAAAAGTCATCAAGGCTCAGATTTGGGATACTGCTGGCCAAGAAAGATTTGGGTACCGCGCCATCACTAGTGCTTACTATCGCGGGGCTGTGGGTGCACTCCTTGTGTACGATGTCACTCGCCATGCGACATTTGAGAATGTCGACAGATGGCTGAAGGAATTGAGGAACCACACCGATTCCAACATTGTTGTGATGCTCGTTGGGAACAAATCAGATCTCCGCCACCTTGTAGCTGTCTCAACTGAAGACGGGAAGTCCTATGCTGAGAAGGAGTCACTCTACTTCATGGAAACATCTGCACTGGAATCAAAGAATGTAGAAAACTCCTTTGCTGAAGTTCTGACTCAAATATACCATACTGTGAGCAAGAAGGCGGTTGAAGGGGGAGAAAATGGAACTTCATCTGTCCCAGCACAAGGAGAGAAAATAAACATGAAAGATGATGTGTCCGCGTTGAAGAGAGTTGGGTGCTGCTCAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0006972 GO:0008104 GO:0008150 GO:0009628 GO:0009651 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017076 GO:0019001 GO:0031090 GO:0031410 GO:0031982 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0034613 GO:0035639 GO:0036094 GO:0042538 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060627 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0097159 GO:0097367 GO:0097708 GO:0098588 GO:0098805 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

220

Amino Acids

24.25

Weight (kDa)

5.87

Isoelectric Point (pI)

22.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	14 - 173	2.9e-11	ADP-ribosylation factor family
Ras	PF00071	15 - 177	1.7e-61	Ras family
Roc	PF08477	15 - 131	1e-33	Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1	PF01926	16 - 123	6.5e-07	50S ribosome-binding GTPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16920
fragaria_vesca	FvH4_3g18190
malus_domestica	MD00G1077000.v1.1 MD03G1275900.v1.1 MD11G1264700.v1.1
prunus_persica	Prupe.4G161600_v2.0.a1
pyrus_communis	pycom03g19300 pycom11g23300
rosa_chinensis	RchiOBHm_Chr5g0030721
rosa_laevigata	RLG00000033247
rosa_multiflora	Rmu_sc0003238.1_g000034
rosa_rugosa	Rorug05G0120400
rosa_samantha	Rh5AG211900 Rh5BG211000 Rh5CG234700 Rh5DG216400
rosa_wichuraiana	Rw0G000930 Rw5G019450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
Acc65I	GGTACC	1 cut(s)	228
AccB1I	GGYRCC	1 cut(s)	228
AccI	GTMKAC	1 cut(s)	318
AccII	CGCG	3 cut(s)	234, 258, 629
AciI	CCGC	5 cut(s)	232, 258, 397, 551, 627
AcoI	YGGCCR	1 cut(s)	211
AcuI	CTGAAG	3 cut(s)	350, 441, 531
AfaI	GTAC	2 cut(s)	230, 284
AfiI	CCNNNNNNNGG	2 cut(s)	72, 593
AgsI	TTSAA	4 cut(s)	43, 115, 557, 634
AhdI	GACNNNNNGTC	1 cut(s)	430
AhlI	ACTAGT	1 cut(s)	242
AjnI	CCWGG	1 cut(s)	155
AjuI	GAANNNNNNNTTGG	2 cut(s)	95, 127
AluBI	AGCT	2 cut(s)	410, 657
AluI	AGCT	2 cut(s)	410, 657
Alw21I	GWGCWC	1 cut(s)	274
Alw26I	GTCTC	1 cut(s)	418
Alw44I	GTGCAC	1 cut(s)	270
Ama87I	CYCGRG	1 cut(s)	119
AoxI	GGCC	1 cut(s)	211
ApaLI	GTGCAC	1 cut(s)	270
ApeKI	GCWGC	1 cut(s)	648
Asp700I	GAANNNNTTC	1 cut(s)	110
Asp718I	GGTACC	1 cut(s)	228
AspLEI	GCGC	1 cut(s)	236
AspS9I	GGNCC	1 cut(s)	159
AsuHPI	GGTGA	1 cut(s)	91
AvaI	CYCGRG	1 cut(s)	119
AvaII	GGWCC	1 cut(s)	159
BaeGI	GKGCMC	1 cut(s)	274
BalI	TGGCCA	1 cut(s)	213
BanI	GGYRCC	1 cut(s)	228
BbsI	GAAGAC	1 cut(s)	429
Bbv12I	GWGCWC	1 cut(s)	274
BbvI	GCAGC	1 cut(s)	635
BccI	CCATC	2 cut(s)	245, 318
BciT130I	CCWGG	1 cut(s)	157
BciVI	GTATCC	1 cut(s)	196
BcoDI	GTCTC	1 cut(s)	418
BcuI	ACTAGT	1 cut(s)	242
BfaI	CTAG	3 cut(s)	93, 243, 658
BfuI	GTATCC	1 cut(s)	196
BglII	AGATCT	1 cut(s)	391
BisI	GCNGC	1 cut(s)	649
BlsI	GCNGC	1 cut(s)	650
Bme1390I	CCNGG	1 cut(s)	157
Bme18I	GGWCC	1 cut(s)	159
BmeRI	GACNNNNNGTC	1 cut(s)	430
BmeT110I	CYCGRG	1 cut(s)	119
BmgT120I	GGNCC	1 cut(s)	159
BmiI	GGNNCC	2 cut(s)	230, 344
BmrFI	CCNGG	1 cut(s)	157
BmsI	GCATC	1 cut(s)	360
BpiI	GAAGAC	1 cut(s)	429
BpuEI	CTTGAG	1 cut(s)	637
Bsc4I	CCNNNNNNNGG	2 cut(s)	72, 593
Bse1I	ACTGG	1 cut(s)	483
BseBI	CCWGG	1 cut(s)	157
BseLI	CCNNNNNNNGG	2 cut(s)	72, 593
BseMII	CTCAG	2 cut(s)	206, 432
BseNI	ACTGG	1 cut(s)	483
BseSI	GKGCMC	1 cut(s)	274
BseXI	GCAGC	1 cut(s)	635
BseYI	CCCAGC	1 cut(s)	586
BsgI	GTGCAG	1 cut(s)	459
Bsh1236I	CGCG	3 cut(s)	234, 258, 629
BshFI	GGCC	1 cut(s)	213
BshNI	GGYRCC	1 cut(s)	228
BsiHKAI	GWGCWC	1 cut(s)	274
BsiHKCI	CYCGRG	1 cut(s)	119
BsiSI	CCGG	1 cut(s)	66
BslFI	GGGAC	2 cut(s)	74, 569
BslI	CCNNNNNNNGG	2 cut(s)	72, 593
BsmAI	GTCTC	1 cut(s)	418
BsmFI	GGGAC	2 cut(s)	74, 569
BsnI	GGCC	1 cut(s)	213
BsoBI	CYCGRG	1 cut(s)	119
Bsp1286I	GDGCHC	1 cut(s)	274
Bsp143I	GATC	1 cut(s)	391
BspACI	CCGC	5 cut(s)	232, 258, 397, 551, 627
BspANI	GGCC	1 cut(s)	213
BspCNI	CTCAG	2 cut(s)	205, 433
BspFNI	CGCG	3 cut(s)	234, 258, 629
BspLI	GGNNCC	2 cut(s)	230, 344
BspT107I	GGYRCC	1 cut(s)	228
BsrI	ACTGG	1 cut(s)	483
BssMI	GATC	1 cut(s)	391
Bst2UI	CCWGG	1 cut(s)	157
Bst4CI	ACNGT	2 cut(s)	169, 538
Bst6I	CTCTTC	2 cut(s)	44, 629
BstC8I	GCNNGC	1 cut(s)	211
BstDEI	CTNAG	2 cut(s)	192, 441
BstFNI	CGCG	3 cut(s)	234, 258, 629
BstHHI	GCGC	1 cut(s)	236
BstKTI	GATC	1 cut(s)	394
BstMAI	GTCTC	1 cut(s)	418
BstMBI	GATC	1 cut(s)	391
BstMWI	GCNNNNNNNGC	2 cut(s)	269, 654
BstNI	CCWGG	1 cut(s)	157
BstSCI	CCNGG	1 cut(s)	155
BstSLI	GKGCMC	1 cut(s)	274
BstUI	CGCG	3 cut(s)	234, 258, 629
BstV1I	GCAGC	1 cut(s)	635
BstV2I	GAAGAC	1 cut(s)	429
BstX2I	RGATCY	1 cut(s)	391
BstYI	RGATCY	1 cut(s)	391
BsuI	GTATCC	1 cut(s)	196
BsuRI	GGCC	1 cut(s)	213
BtsIMutI	CAGTG	1 cut(s)	476
Cac8I	GCNNGC	1 cut(s)	211
CfoI	GCGC	1 cut(s)	236
Cfr13I	GGNCC	1 cut(s)	159
Csp6I	GTAC	2 cut(s)	229, 283
CviAII	CATG	3 cut(s)	299, 463, 610
CviJI	RGCY	8 cut(s)	5, 17, 191, 213, 263, 328, 410, 657
CviKI_1	RGCY	8 cut(s)	5, 17, 191, 213, 263, 328, 410, 657
CviQI	GTAC	2 cut(s)	229, 283
DdeI	CTNAG	2 cut(s)	192, 441
DpnI	GATC	1 cut(s)	393
DpnII	GATC	1 cut(s)	391
DrdI	GACNNNNNNGTC	1 cut(s)	179
DriI	GACNNNNNGTC	1 cut(s)	430
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	1 cut(s)	211
Eam1104I	CTCTTC	2 cut(s)	44, 629
Eam1105I	GACNNNNNGTC	1 cut(s)	430
EarI	CTCTTC	2 cut(s)	44, 629
EciI	GGCGGA	1 cut(s)	386
Eco47I	GGWCC	1 cut(s)	159
Eco57I	CTGAAG	3 cut(s)	350, 441, 531
Eco88I	CYCGRG	1 cut(s)	119
EcoO109I	RGGNCCY	1 cut(s)	159
EcoRII	CCWGG	1 cut(s)	155
FaeI	CATG	3 cut(s)	302, 466, 613
FaiI	YATR	7 cut(s)	30, 300, 438, 464, 529, 534, 611
FalI	AAGNNNNNCTT	2 cut(s)	35, 67
FaqI	GGGAC	2 cut(s)	74, 569
FatI	CATG	3 cut(s)	298, 462, 609
FauI	CCCGC	1 cut(s)	251
FblI	GTMKAC	1 cut(s)	318
Fnu4HI	GCNGC	1 cut(s)	649
Fsp4HI	GCNGC	1 cut(s)	649
FspBI	CTAG	3 cut(s)	93, 243, 658
GlaI	GCGC	1 cut(s)	235
GluI	GCNGC	1 cut(s)	649
GsaI	CCCAGC	1 cut(s)	590
HaeIII	GGCC	1 cut(s)	213
HapII	CCGG	1 cut(s)	66
HhaI	GCGC	1 cut(s)	236
Hin1II	CATG	3 cut(s)	302, 466, 613
Hin6I	GCGC	1 cut(s)	234
HinP1I	GCGC	1 cut(s)	234
HincII	GTYRAC	2 cut(s)	172, 319
HindII	GTYRAC	2 cut(s)	172, 319
HinfI	GANTC	7 cut(s)	62, 82, 122, 353, 449, 482, 520
HpaII	CCGG	1 cut(s)	66
HphI	GGTGA	1 cut(s)	91
Hpy166II	GTNNAC	5 cut(s)	99, 172, 272, 283, 319
Hpy188I	TCNGA	3 cut(s)	195, 391, 519
Hpy188III	TCNNGA	1 cut(s)	52
Hpy8I	GTNNAC	5 cut(s)	99, 172, 272, 283, 319
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	4 cut(s)	325, 439, 541, 551
HpyCH4III	ACNGT	2 cut(s)	169, 538
HpyCH4V	TGCA	2 cut(s)	272, 476
HpyF10VI	GCNNNNNNNGC	2 cut(s)	269, 654
HpyF3I	CTNAG	2 cut(s)	192, 441
Hsp92II	CATG	3 cut(s)	302, 466, 613
HspAI	GCGC	1 cut(s)	234
KpnI	GGTACC	1 cut(s)	232
Kzo9I	GATC	1 cut(s)	391
LpnPI	CCDG	6 cut(s)	79, 142, 169, 195, 464, 600
Lsp1109I	GCAGC	1 cut(s)	635
LweI	GCATC	1 cut(s)	360
MaeI	CTAG	3 cut(s)	93, 243, 658
MaeIII	GTNAC	2 cut(s)	289, 450
MalI	GATC	1 cut(s)	393
MboI	GATC	1 cut(s)	391
MboII	GAAGA	3 cut(s)	31, 434, 646
MflI	RGATCY	1 cut(s)	391
MhlI	GDGCHC	1 cut(s)	274
MlsI	TGGCCA	1 cut(s)	213
MluCI	AATT	1 cut(s)	335
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	4 cut(s)	56, 131, 458, 514
MmeI	TCCRAC	1 cut(s)	381
MnlI	CCTC	3 cut(s)	47, 128, 333
Mox20I	TGGCCA	1 cut(s)	213
MroXI	GAANNNNTTC	1 cut(s)	110
MscI	TGGCCA	1 cut(s)	213
MslI	CAYNNNNRTG	1 cut(s)	365
Msp20I	TGGCCA	1 cut(s)	213
MspI	CCGG	1 cut(s)	66
MspR9I	CCNGG	1 cut(s)	157
MvaI	CCWGG	1 cut(s)	157
MvnI	CGCG	3 cut(s)	234, 258, 629
MwoI	GCNNNNNNNGC	2 cut(s)	269, 654
NdeII	GATC	1 cut(s)	391
NlaIII	CATG	3 cut(s)	302, 466, 613
NlaIV	GGNNCC	2 cut(s)	230, 344
NmuCI	GTSAC	2 cut(s)	289, 450
PaeR7I	CTCGAG	1 cut(s)	119
PdmI	GAANNNNTTC	1 cut(s)	110
PfeI	GAWTC	3 cut(s)	82, 353, 482
PkrI	GCNGC	1 cut(s)	650
PleI	GAGTC	4 cut(s)	56, 130, 457, 514
PpsI	GAGTC	4 cut(s)	56, 130, 457, 514
PpuMI	RGGWCCY	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	159
Psp6I	CCWGG	1 cut(s)	155
PspFI	CCCAGC	1 cut(s)	586
PspGI	CCWGG	1 cut(s)	155
PspN4I	GGNNCC	2 cut(s)	230, 344
PspPI	GGNCC	1 cut(s)	159
PspPPI	RGGWCCY	1 cut(s)	159
PspXI	VCTCGAGB	1 cut(s)	119
PsuI	RGATCY	1 cut(s)	391
RsaI	GTAC	2 cut(s)	230, 284
RsaNI	GTAC	2 cut(s)	229, 283
RseI	CAYNNNNRTG	1 cut(s)	365
SalI	GTCGAC	1 cut(s)	317
SatI	GCNGC	1 cut(s)	649
Sau3AI	GATC	1 cut(s)	391
Sau96I	GGNCC	1 cut(s)	159
SchI	GAGTC	4 cut(s)	56, 131, 458, 514
ScrFI	CCNGG	1 cut(s)	157
SduI	GDGCHC	1 cut(s)	274
SetI	ASST	7 cut(s)	39, 98, 120, 164, 405, 412, 659
SfaNI	GCATC	1 cut(s)	360
Sfr274I	CTCGAG	1 cut(s)	119
SinI	GGWCC	1 cut(s)	159
SlaI	CTCGAG	1 cut(s)	119
SmiMI	CAYNNNNRTG	1 cut(s)	365
SmlI	CTYRAG	2 cut(s)	119, 652
SmoI	CTYRAG	2 cut(s)	119, 652
SpeI	ACTAGT	1 cut(s)	242
Sse9I	AATT	1 cut(s)	335
SsiI	CCGC	5 cut(s)	232, 258, 397, 551, 627
SspMI	CTAG	3 cut(s)	93, 243, 658
StyD4I	CCNGG	1 cut(s)	155
TaaI	ACNGT	2 cut(s)	169, 538
TaqI	TCGA	3 cut(s)	80, 120, 318
TasI	AATT	1 cut(s)	335
TfiI	GAWTC	3 cut(s)	82, 353, 482
TscAI	CASTG	1 cut(s)	483
TseFI	GTSAC	2 cut(s)	289, 450
TseI	GCWGC	1 cut(s)	648
Tsp45I	GTSAC	2 cut(s)	289, 450
TspDTI	ATGAA	3 cut(s)	451, 567, 626
TspGWI	ACGGA	1 cut(s)	189
TspRI	CASTG	1 cut(s)	483
VneI	GTGCAC	1 cut(s)	270
VpaK11BI	GGWCC	1 cut(s)	159
XcmI	CCANNNNNNNNNTGG	1 cut(s)	221
XhoI	CTCGAG	1 cut(s)	119
XmiI	GTMKAC	1 cut(s)	318
XmnI	GAANNNNTTC	1 cut(s)	110
XspI	CTAG	3 cut(s)	93, 243, 658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.