MD04G1082900.v1.1

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0021123
Physical Location & Seq
Reverse (-)
11623187 .. 11624681
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1082900.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGAGAAAAAAGTTGCTGCTTCGAAATTGTTGGCCAAGAAAGGTGAAGACTCCTTGCTCTGTGATGTGGGTCTTTGTTCTTTTCCTGAAGTAGGTTCTTTGCTTGAAGTGGTTTTAATTTCGGTTGCAAAATCTGGTGAAAATGATGTCCACCATGTGTTTGTTGGACTGCAGCAAACAATTGACATTGCATCTTTGAAGCGTGGCTTCCATGAATTCACCCGTGACAAAGGCGGAACAACTATTCAAAGGCTCTTTCATTCGAAATCAGCGACTATTCTTGAATCTGATTGTAAGAATGGCAGATCCCAAGTTTTCATTTTGGAAATTCATGTTTCTAATGTTGAGTTCAAGAAAGCTAAATTGCCTGGTTCGTGTGCACTTTACCTGTTCGACAAAATGTCAAACTCAGGTTACATTCTTGATATTAAGTTGGGGTATCATATGAGCATTCGTGATAAAGGTGGTACAAATTTTCTCTTTAGTTTTGAATCAATAGATTTACTGGCAAGCTACAAGGACTCAGGCTTCTGTGTTGTTATTGATCGTTCCATGGGCTACTGCTGCAAGGGAATTCGTGACATGGGTGGAACTATTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

201

Amino Acids

21.96

Weight (kDa)

8.12

Isoelectric Point (pI)

28.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021123)

Species Orthologous Gene IDs
malus_domestica MD04G1082900.v1.1 MD10G1188900.v1.1 MD11G1266500.v1.1 MD13G1252100.v1.1
pyrus_communis pycom17g05370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 234
AclWI GGATC 1 cut(s) 300
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 4 cut(s) 215, 327, 472, 573
AcuI CTGAAG 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 6 cut(s) 107, 199, 248, 284, 352, 491
AhdI GACNNNNNGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 2 cut(s) 359, 513
AluI AGCT 2 cut(s) 359, 513
Alw21I GWGCWC 1 cut(s) 382
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 1 cut(s) 300
AoxI GGCC 1 cut(s) 33
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 3 cut(s) 17, 172, 564
ApoI RAATTY 4 cut(s) 215, 327, 472, 573
AsuHPI GGTGA 3 cut(s) 56, 149, 211
AsuII TTCGAA 2 cut(s) 23, 263
BaeGI GKGCMC 1 cut(s) 382
BalI TGGCCA 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 3 cut(s) 4, 184, 551
BciT130I CCWGG 1 cut(s) 369
BfmI CTRYAG 1 cut(s) 170
BisI GCNGC 3 cut(s) 18, 173, 565
BlsI GCNGC 3 cut(s) 19, 174, 566
Bme1390I CCNGG 1 cut(s) 369
BmeRI GACNNNNNGTC 1 cut(s) 400
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 54
Bpu14I TTCGAA 2 cut(s) 23, 263
BsaJI CCNNGG 1 cut(s) 552
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 552
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 423, 537
BseNI ACTGG 1 cut(s) 510
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 3 cut(s) 4, 184, 551
BshFI GGCC 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 92
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 1 cut(s) 35
Bsp119I TTCGAA 2 cut(s) 23, 263
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 305, 544
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 1 cut(s) 234
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 422, 536
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 1 cut(s) 300
BspT104I TTCGAA 2 cut(s) 23, 263
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 2 cut(s) 305, 544
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 369
BstBI TTCGAA 2 cut(s) 23, 263
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 2 cut(s) 409, 523
BstDSI CCRYGG 1 cut(s) 552
BstENI CCTNNNNNAGG 1 cut(s) 90
BstKTI GATC 2 cut(s) 308, 547
BstMBI GATC 2 cut(s) 305, 544
BstMWI GCNNNNNNNGC 1 cut(s) 564
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 3 cut(s) 4, 184, 551
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 305
BstYI RGATCY 1 cut(s) 305
BsuRI GGCC 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 552
Cac8I GCNNGC 1 cut(s) 511
Csp6I GTAC 1 cut(s) 468
CviAII CATG 5 cut(s) 155, 212, 332, 553, 583
CviJI RGCY 7 cut(s) 35, 207, 253, 359, 513, 528, 558
CviKI_1 RGCY 7 cut(s) 35, 207, 253, 359, 513, 528, 558
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 2 cut(s) 409, 523
DpnI GATC 2 cut(s) 307, 546
DpnII GATC 2 cut(s) 305, 544
DraIII CACNNNGTG 1 cut(s) 157
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 33
Eam1105I GACNNNNNGTC 1 cut(s) 400
EciI GGCGGA 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 90
EcoRI GAATTC 2 cut(s) 215, 573
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 5 cut(s) 158, 215, 335, 556, 586
FaiI YATR 7 cut(s) 156, 213, 333, 444, 446, 554, 584
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FatI CATG 5 cut(s) 154, 211, 331, 552, 582
FauNDI CATATG 1 cut(s) 444
Fnu4HI GCNGC 3 cut(s) 18, 173, 565
Fsp4HI GCNGC 3 cut(s) 18, 173, 565
GluI GCNGC 3 cut(s) 18, 173, 565
HaeIII GGCC 1 cut(s) 35
Hin1II CATG 5 cut(s) 158, 215, 335, 556, 586
HinfI GANTC 4 cut(s) 50, 284, 491, 521
HphI GGTGA 3 cut(s) 56, 149, 211
Hpy166II GTNNAC 2 cut(s) 151, 380
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 6 cut(s) 86, 281, 352, 422, 455, 578
Hpy8I GTNNAC 2 cut(s) 151, 380
HpyCH4V TGCA 5 cut(s) 128, 172, 191, 380, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 564
HpyF3I CTNAG 2 cut(s) 409, 523
Hsp92II CATG 5 cut(s) 158, 215, 335, 556, 586
Kzo9I GATC 2 cut(s) 305, 544
LpnPI CCDG 8 cut(s) 99, 120, 354, 381, 396, 401, 491, 510
Lsp1109I GCAGC 3 cut(s) 4, 184, 551
LweI GCATC 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 224, 413, 578
MalI GATC 2 cut(s) 307, 546
MboI GATC 2 cut(s) 305, 544
MboII GAAGA 1 cut(s) 59
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 1 cut(s) 305
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 8 cut(s) 26, 117, 180, 215, 327, 362, 472, 573
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 2 cut(s) 44, 515
MmeI TCCRAC 1 cut(s) 145
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 2 cut(s) 116, 429
Msp20I TGGCCA 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 564
NcoI CCATGG 1 cut(s) 552
NdeI CATATG 1 cut(s) 444
NdeII GATC 2 cut(s) 305, 544
NlaIII CATG 5 cut(s) 158, 215, 335, 556, 586
NmuCI GTSAC 2 cut(s) 224, 578
NspV TTCGAA 2 cut(s) 23, 263
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 284, 491
PkrI GCNGC 3 cut(s) 19, 174, 566
PleI GAGTC 2 cut(s) 44, 515
PpsI GAGTC 2 cut(s) 44, 515
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SaqAI TTAA 2 cut(s) 116, 429
SatI GCNGC 3 cut(s) 18, 173, 565
Sau3AI GATC 2 cut(s) 305, 544
SchI GAGTC 2 cut(s) 44, 515
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 1 cut(s) 382
SetI ASST 7 cut(s) 46, 97, 361, 390, 415, 466, 515
SfaNI GCATC 1 cut(s) 200
SfcI CTRYAG 1 cut(s) 170
SfuI TTCGAA 2 cut(s) 23, 263
Sse9I AATT 8 cut(s) 26, 117, 180, 215, 327, 362, 472, 573
SsiI CCGC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 552
TaqI TCGA 3 cut(s) 23, 263, 393
TasI AATT 8 cut(s) 26, 117, 180, 215, 327, 362, 472, 573
TfiI GAWTC 2 cut(s) 284, 491
Tru1I TTAA 2 cut(s) 116, 429
Tru9I TTAA 2 cut(s) 116, 429
TseFI GTSAC 2 cut(s) 224, 578
TseI GCWGC 3 cut(s) 17, 172, 564
Tsp45I GTSAC 2 cut(s) 224, 578
TspDTI ATGAA 4 cut(s) 228, 248, 307, 320
VneI GTGCAC 1 cut(s) 378
XagI CCTNNNNNAGG 1 cut(s) 90
XapI RAATTY 4 cut(s) 215, 327, 472, 573
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.