MD10G1188900.v1.1

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0021123
Physical Location & Seq
Reverse (-)
28516888 .. 28518711
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1188900.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGAATCGAGGGTTTCCAATCTGGCAGCCCAGTTCGACGAATTCAAGAAGTCTCACGACTTGATTCTTCTTCAATTGCGGTCCCTCATGGCTAAGGGTCACGATTCAATATCGAATTCGAGGCAACCATTCTTCAAAATGGCGTCTCATATCTCTGTTGTGAAATCGGATGAAATTGTTCTCAAGCTTCAATCCTTCCACAACGAAAAGATTCAAGCCCGTGAGAAAGTAGATGAATTCTTGTCTCAGTACCGTCCAAAATACAAGCCAAAAAGTCCTCCTTGTTTACGTCCAACATTGAAGAAATTCAAGAGGGTTCGCGACTATGCCAAATACCACGCAGATGGTTCTGCAATATTTCATTCCCGTTCCAAATTTCAAGAAAGTTTTGGGTTCTTTCGAAGTGAGTTGGCTAGGAAGAATGAACAGGTTTTCGATTTACTCGACAAGCAAGTTGCCAATTCTGTGAAAAGTCCTCCATTTCCTTATTTTGCTTGCAAGAAGGACACGGCAAGGGATTTGGTTTCTGGGTTTCCTATTTCAGATTTGTTAGAGAAGAAAGATGAATTGCCTTCTTCTCTGTCTGTTCTCGAACTTGTTGCTTCGAAATTGTTGGGCAAGTTAGGTGACGACTCCTTGCTCTGTGATCAAACGTTCGACATTGCTTCTTTGACGCTTGGCTTCCATGAATTCACTCGTGACAAAGGCGGAACAAATATTCAAAGGCTCTTTCATTCGAAATCAGCGACTGTTCTTGAATCTGATGGTAAGAATGGCAAATCCCAAGTTTTCATTTTAGGAATTCATGTTTCTAATGTTGAGTTCAAGAAAGCTAAATTGCCTGGTTCGCGTGCACTCTACCTGTTCGACAAAATGTCGTATTCAGGTTACATTCTTGATAATAAGCTTGGGTACCTTATGAGCATTCGTGATAAAGGTGGTACTAATTTTCTCTCCAGTTTTGAGTCAATAGATTTACTGGCAAGCTGCAAGGACTTAGGTTTTTGTGTTGATATCAATCGTTCCATGGGCTACTACTGCAAGGGAATTCGTGACAAGGGTGGAACAATTAGTCAGAGGCTCTTCCCTTCGAAGTCCAAGAATCAAGATTGTGTTATGGGTTCTGATTGTTCCAAGGTGATCAAATTATGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

386

Amino Acids

43.5

Weight (kDa)

9.35

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021123)

Species Orthologous Gene IDs
malus_domestica MD04G1082900.v1.1 MD10G1188900.v1.1 MD11G1266500.v1.1 MD13G1252100.v1.1
pyrus_communis pycom17g05370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 912
AccB1I GGYRCC 1 cut(s) 912
AccII CGCG 2 cut(s) 321, 850
AciI CCGC 2 cut(s) 79, 708
AclI AACGTT 1 cut(s) 653
AcsI RAATTY 8 cut(s) 41, 115, 236, 305, 374, 689, 801, 1047
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 3 cut(s) 251, 914, 943
AhdI GACNNNNNGTC 1 cut(s) 874
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 4 cut(s) 187, 833, 907, 987
AluI AGCT 4 cut(s) 187, 833, 907, 987
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 3 cut(s) 57, 150, 249
Alw44I GTGCAC 1 cut(s) 852
AlwNI CAGNNNCTG 1 cut(s) 749
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 2 cut(s) 26, 987
ApoI RAATTY 8 cut(s) 41, 115, 236, 305, 374, 689, 801, 1047
Asp700I GAANNNNTTC 3 cut(s) 177, 210, 305
Asp718I GGTACC 1 cut(s) 912
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 638, 1150
AsuII TTCGAA 4 cut(s) 400, 605, 737, 1091
AvaII GGWCC 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 912
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 2 cut(s) 38, 974
BccI CCATC 2 cut(s) 338, 758
BceAI ACGGC 1 cut(s) 525
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 1 cut(s) 843
BclI TGATCA 2 cut(s) 646, 1140
BcoDI GTCTC 3 cut(s) 57, 150, 249
BfaI CTAG 1 cut(s) 414
BisI GCNGC 2 cut(s) 27, 988
BlsI GCNGC 2 cut(s) 28, 989
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 1 cut(s) 81
BmeRI GACNNNNNGTC 1 cut(s) 874
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 83, 914
BmrFI CCNGG 1 cut(s) 843
BmrI ACTGGG 1 cut(s) 25
BmuI ACTGGG 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 940
Bpu10I CCTNAGC 1 cut(s) 93
Bpu14I TTCGAA 4 cut(s) 400, 605, 737, 1091
BpuEI CTTGAG 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 1026, 1134
Bse1I ACTGG 3 cut(s) 31, 957, 984
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 1026, 1134
BseGI GGATG 1 cut(s) 175
BseJI GATNNNNATC 1 cut(s) 1017
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 3 cut(s) 31, 957, 984
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 2 cut(s) 38, 974
Bsh1236I CGCG 2 cut(s) 321, 850
BshNI GGYRCC 1 cut(s) 912
BsiHKAI GWGCWC 1 cut(s) 856
BslFI GGGAC 1 cut(s) 67
BsmAI GTCTC 3 cut(s) 57, 150, 249
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 924
Bsp119I TTCGAA 4 cut(s) 400, 605, 737, 1091
Bsp1286I GDGCHC 1 cut(s) 856
Bsp143I GATC 2 cut(s) 646, 1140
Bsp19I CCATGG 1 cut(s) 1026
Bsp68I TCGCGA 1 cut(s) 321
BspACI CCGC 2 cut(s) 79, 708
BspCNI CTCAG 1 cut(s) 259
BspFNI CGCG 2 cut(s) 321, 850
BspLI GGNNCC 2 cut(s) 83, 914
BspQI GCTCTTC 1 cut(s) 1088
BspT104I TTCGAA 4 cut(s) 400, 605, 737, 1091
BspT107I GGYRCC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 3 cut(s) 31, 957, 984
BssECI CCNNGG 2 cut(s) 1026, 1134
BssMI GATC 2 cut(s) 646, 1140
BssNI GRCGYC 1 cut(s) 143
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 2 cut(s) 1026, 1134
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 2 cut(s) 254, 751
Bst6I CTCTTC 1 cut(s) 1088
BstACI GRCGYC 1 cut(s) 143
BstBI TTCGAA 4 cut(s) 400, 605, 737, 1091
BstC8I GCNNGC 3 cut(s) 496, 852, 985
BstDEI CTNAG 3 cut(s) 93, 246, 997
BstDSI CCRYGG 1 cut(s) 1026
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 2 cut(s) 321, 850
BstKTI GATC 2 cut(s) 649, 1143
BstMAI GTCTC 3 cut(s) 57, 150, 249
BstMBI GATC 2 cut(s) 646, 1140
BstMWI GCNNNNNNNGC 2 cut(s) 847, 1038
BstNI CCWGG 1 cut(s) 843
BstSCI CCNGG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 856
BstUI CGCG 2 cut(s) 321, 850
BstV1I GCAGC 2 cut(s) 38, 974
BstXI CCANNNNNNTGG 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 1026
BtsCI GGATG 1 cut(s) 175
BtuMI TCGCGA 1 cut(s) 321
Cac8I GCNNGC 3 cut(s) 496, 852, 985
CaiI CAGNNNCTG 1 cut(s) 749
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 2 cut(s) 132, 682
Csp6I GTAC 3 cut(s) 250, 913, 942
CviAII CATG 4 cut(s) 88, 686, 806, 1027
CviQI GTAC 3 cut(s) 250, 913, 942
DdeI CTNAG 3 cut(s) 93, 246, 997
DpnI GATC 2 cut(s) 648, 1142
DpnII GATC 2 cut(s) 646, 1140
DriI GACNNNNNGTC 1 cut(s) 874
Eam1104I CTCTTC 1 cut(s) 1088
Eam1105I GACNNNNNGTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 1088
EciI GGCGGA 1 cut(s) 723
Eco130I CCWWGG 2 cut(s) 1026, 1134
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 1 cut(s) 81
EcoRI GAATTC 6 cut(s) 41, 115, 236, 689, 801, 1047
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 2 cut(s) 1026, 1134
ErhI CCWWGG 2 cut(s) 1026, 1134
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 4 cut(s) 91, 689, 809, 1030
FaiI YATR 9 cut(s) 89, 150, 327, 687, 807, 920, 1028, 1118, 1150
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 87, 685, 805, 1026
FbaI TGATCA 2 cut(s) 646, 1140
Fnu4HI GCNGC 2 cut(s) 27, 988
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 27, 988
FspBI CTAG 1 cut(s) 414
GluI GCNGC 2 cut(s) 27, 988
GsuI CTGGAG 1 cut(s) 940
HgaI GACGC 2 cut(s) 132, 682
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 4 cut(s) 91, 689, 809, 1030
HindIII AAGCTT 2 cut(s) 185, 905
HinfI GANTC 8 cut(s) 5, 64, 104, 211, 632, 758, 965, 1102
HphI GGTGA 2 cut(s) 638, 1150
Hpy166II GTNNAC 2 cut(s) 287, 854
Hpy188I TCNGA 5 cut(s) 169, 544, 763, 1077, 1126
Hpy8I GTNNAC 2 cut(s) 287, 854
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 205, 496, 582, 1098
HpyCH4III ACNGT 2 cut(s) 254, 751
HpyCH4IV ACGT 2 cut(s) 289, 653
HpyCH4V TGCA 5 cut(s) 353, 498, 854, 990, 1041
HpyF10VI GCNNNNNNNGC 2 cut(s) 847, 1038
HpyF3I CTNAG 3 cut(s) 93, 246, 997
HpySE526I ACGT 2 cut(s) 289, 653
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 4 cut(s) 91, 689, 809, 1030
KpnI GGTACC 1 cut(s) 916
Ksp22I TGATCA 2 cut(s) 646, 1140
Kzo9I GATC 2 cut(s) 646, 1140
LguI GCTCTTC 1 cut(s) 1088
Lsp1109I GCAGC 2 cut(s) 38, 974
MaeI CTAG 1 cut(s) 414
MaeII ACGT 2 cut(s) 289, 653
MaeIII GTNAC 5 cut(s) 98, 626, 698, 887, 1052
MalI GATC 2 cut(s) 648, 1142
MboI GATC 2 cut(s) 646, 1140
MboII GAAGA 8 cut(s) 59, 62, 124, 313, 430, 567, 568, 1075
MfeI CAATTG 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 856
MlyI GAGTC 2 cut(s) 626, 974
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 7 cut(s) 3, 95, 114, 288, 306, 486, 1071
MroXI GAANNNNTTC 3 cut(s) 177, 210, 305
MslI CAYNNNNRTG 1 cut(s) 342
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 1 cut(s) 74
Mva1269I GAATGC 1 cut(s) 924
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 2 cut(s) 321, 850
MwoI GCNNNNNNNGC 2 cut(s) 847, 1038
NcoI CCATGG 1 cut(s) 1026
NdeII GATC 2 cut(s) 646, 1140
NlaIII CATG 4 cut(s) 91, 689, 809, 1030
NlaIV GGNNCC 2 cut(s) 83, 914
NmuCI GTSAC 4 cut(s) 98, 626, 698, 1052
NruI TCGCGA 1 cut(s) 321
NspV TTCGAA 4 cut(s) 400, 605, 737, 1091
PciSI GCTCTTC 1 cut(s) 1088
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 743
PctI GAATGC 1 cut(s) 924
PdmI GAANNNNTTC 3 cut(s) 177, 210, 305
PfeI GAWTC 6 cut(s) 5, 64, 104, 211, 758, 1102
PkrI GCNGC 2 cut(s) 28, 989
PleI GAGTC 2 cut(s) 626, 973
PpsI GAGTC 2 cut(s) 626, 973
Psp1406I AACGTT 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 2 cut(s) 83, 914
PspPI GGNCC 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 749
RruI TCGCGA 1 cut(s) 321
RsaI GTAC 3 cut(s) 251, 914, 943
RsaNI GTAC 3 cut(s) 250, 913, 942
RseI CAYNNNNRTG 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 1088
SatI GCNGC 2 cut(s) 27, 988
Sau3AI GATC 2 cut(s) 646, 1140
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 2 cut(s) 626, 974
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 1 cut(s) 856
SfuI TTCGAA 4 cut(s) 400, 605, 737, 1091
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SsiI CCGC 2 cut(s) 79, 708
SspI AATATT 2 cut(s) 357, 718
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 2 cut(s) 1026, 1134
TaaI ACNGT 2 cut(s) 254, 751
TaiI ACGT 2 cut(s) 292, 656
TfiI GAWTC 6 cut(s) 5, 64, 104, 211, 758, 1102
TseFI GTSAC 4 cut(s) 98, 626, 698, 1052
TseI GCWGC 2 cut(s) 26, 987
Tsp45I GTSAC 4 cut(s) 98, 626, 698, 1052
TspDTI ATGAA 9 cut(s) 186, 249, 350, 438, 579, 702, 722, 781, 794
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 8 cut(s) 41, 115, 236, 305, 374, 689, 801, 1047
XmnI GAANNNNTTC 3 cut(s) 177, 210, 305
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.