MD04G1110100.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0022397
Physical Location & Seq
Forward (+)
19486631 .. 19487459
829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1110100.v1.1.491

Sequence Viewer

Length: 627 bp
ATGCCGCGAGGTCAAACATTCTCCACCGTAATACTCACCACCTCTCTCTTGATTCTCGCGGTCTCAGCCAACTTGAGAAATCACATTCTTCATGTCAATCATGTTGACACGACGAACGCTGATGCTAATCCCAATTATGAATCACACGACATAGCTAATGCCGTCGACCACATTGGCAAGATCGTCGGCGGCATTGCCAACGACGCCAACAGCCCCAACAGCCTTGTCAATAACCTCGCCAGCGCGTTGAAACCAAACAAGGACAACTTCACCGAGTATACTCGGGCGGCGCTGGTCCAAGAGTTCATCATGGCCCATAACGAGATCCGAAGGGCAGAGAACATGAAGCCGTTGGTGTGGAACGACACGATGGCCCAGTTGGCGGAGGACTGGGCCAAGAAGCAGGATCACGACTGCGGAATGCGGCACTCGTCGTATCAAGGTATGGGGGAGAACTTGTTCTGGGGTTACAAAGCGCATTGGCTGCCGAGGGAGGCAGTGTATGATTGGGCACATGAGAAGCAGTACTACAACAAAGAAAACCGCGAGTGCGAGCCAGACAAAGCATGTGACCACTACACGCAGATCATATGGAAAGACACTGAACAAGTAGGATGCGCCCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

209

Amino Acids

23.72

Weight (kDa)

5.85

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 105 - 207 3.6e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022397)

Species Orthologous Gene IDs
malus_domestica MD04G1110100.v1.1 MD12G1131300.v1.1
prunus_persica Prupe.6G245700_v2.0.a1
pyrus_communis pycom12g12700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 165, 278
AccII CGCG 4 cut(s) 7, 59, 245, 546
AciI CCGC 8 cut(s) 5, 59, 189, 287, 383, 417, 424, 544
AclWI GGATC 2 cut(s) 319, 414
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 1 cut(s) 382
AgsI TTSAA 1 cut(s) 250
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 319, 414
Ama87I CYCGRG 1 cut(s) 282
AoxI GGCC 3 cut(s) 312, 372, 393
ApeKI GCWGC 1 cut(s) 484
Asp700I GAANNNNTTC 1 cut(s) 458
AspLEI GCGC 4 cut(s) 245, 292, 478, 620
AspS9I GGNCC 4 cut(s) 295, 313, 373, 393
AsuHPI GGTGA 2 cut(s) 28, 262
AvaI CYCGRG 1 cut(s) 282
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 514
BarI GAAGNNNNNNTAC 2 cut(s) 512, 544
BbvI GCAGC 1 cut(s) 471
BccI CCATC 1 cut(s) 364
BceAI ACGGC 2 cut(s) 146, 334
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BcoDI GTCTC 1 cut(s) 67
BfoI RGCGCY 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 5 cut(s) 5, 190, 288, 425, 485
BlsI GCNGC 5 cut(s) 6, 191, 289, 426, 486
BmcAI AGTACT 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 282
BmgT120I GGNCC 4 cut(s) 295, 313, 373, 393
BmrI ACTGGG 2 cut(s) 370, 400
BmsI GCATC 2 cut(s) 112, 605
BmuI ACTGGG 2 cut(s) 370, 400
BpuEI CTTGAG 1 cut(s) 94
BsaBI GATNNNNATC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 488
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 485, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 376, 395
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 488
BseGI GGATG 1 cut(s) 620
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 376, 395
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 1 cut(s) 471
Bsh1236I CGCG 4 cut(s) 7, 59, 245, 546
BshFI GGCC 3 cut(s) 314, 374, 395
BsiHKCI CYCGRG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 3 cut(s) 314, 374, 395
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 4 cut(s) 180, 324, 406, 585
BspACI CCGC 8 cut(s) 5, 59, 189, 287, 383, 417, 424, 544
BspANI GGCC 3 cut(s) 314, 374, 395
BspCNI CTCAG 1 cut(s) 77
BspFNI CGCG 4 cut(s) 7, 59, 245, 546
BspPI GGATC 2 cut(s) 319, 414
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 2 cut(s) 376, 395
BssECI CCNNGG 1 cut(s) 488
BssMI GATC 4 cut(s) 180, 324, 406, 585
BssNAI GTATAC 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 28
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 241, 554
BstDEI CTNAG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 620
BstFNI CGCG 4 cut(s) 7, 59, 245, 546
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 4 cut(s) 245, 292, 478, 620
BstKTI GATC 4 cut(s) 183, 327, 409, 588
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 4 cut(s) 180, 324, 406, 585
BstMWI GCNNNNNNNGC 5 cut(s) 65, 203, 219, 380, 484
BstNSI RCATGY 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 4 cut(s) 7, 59, 245, 546
BstV1I GCAGC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BstZ17I GTATAC 1 cut(s) 279
BsuRI GGCC 3 cut(s) 314, 374, 395
BtsCI GGATG 1 cut(s) 620
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 504, 600
Cac8I GCNNGC 2 cut(s) 241, 554
CfoI GCGC 4 cut(s) 245, 292, 478, 620
Cfr13I GGNCC 4 cut(s) 295, 313, 373, 393
CseI GACGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 526
CviAII CATG 6 cut(s) 92, 101, 310, 343, 515, 567
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 64
DpnI GATC 4 cut(s) 182, 326, 408, 587
DpnII GATC 4 cut(s) 180, 324, 406, 585
EciI GGCGGA 1 cut(s) 398
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 282
FaeI CATG 6 cut(s) 95, 104, 313, 346, 518, 570
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 6 cut(s) 91, 100, 309, 342, 514, 566
FauNDI CATATG 1 cut(s) 590
FblI GTMKAC 2 cut(s) 165, 278
Fnu4HI GCNGC 5 cut(s) 5, 190, 288, 425, 485
Fsp4HI GCNGC 5 cut(s) 5, 190, 288, 425, 485
GlaI GCGC 4 cut(s) 244, 291, 477, 619
GluI GCNGC 5 cut(s) 5, 190, 288, 425, 485
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 3 cut(s) 314, 374, 395
HgaI GACGC 1 cut(s) 212
HhaI GCGC 4 cut(s) 245, 292, 478, 620
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 6 cut(s) 95, 104, 313, 346, 518, 570
Hin6I GCGC 4 cut(s) 243, 290, 476, 618
HinP1I GCGC 4 cut(s) 243, 290, 476, 618
HincII GTYRAC 2 cut(s) 106, 166
HindII GTYRAC 2 cut(s) 106, 166
HinfI GANTC 2 cut(s) 52, 140
HphI GGTGA 2 cut(s) 28, 262
Hpy166II GTNNAC 3 cut(s) 106, 166, 279
Hpy188I TCNGA 1 cut(s) 329
Hpy188III TCNNGA 2 cut(s) 49, 410
Hpy8I GTNNAC 3 cut(s) 106, 166, 279
Hpy99I CGWCG 5 cut(s) 115, 167, 188, 206, 436
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 1 cut(s) 28
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 203, 219, 380, 484
HpyF3I CTNAG 1 cut(s) 64
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 6 cut(s) 95, 104, 313, 346, 518, 570
HspAI GCGC 4 cut(s) 243, 290, 476, 618
Kzo9I GATC 4 cut(s) 180, 324, 406, 585
LpnPI CCDG 7 cut(s) 253, 278, 376, 389, 389, 448, 570
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 2 cut(s) 112, 605
MaeIII GTNAC 2 cut(s) 467, 569
MalI GATC 4 cut(s) 182, 326, 408, 587
MboI GATC 4 cut(s) 180, 324, 406, 585
MboII GAAGA 1 cut(s) 80
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 1 cut(s) 133
MnlI CCTC 5 cut(s) 52, 245, 379, 483, 487
MroXI GAANNNNTTC 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 426
MvnI CGCG 4 cut(s) 7, 59, 245, 546
MwoI GCNNNNNNNGC 5 cut(s) 65, 203, 219, 380, 484
NdeI CATATG 1 cut(s) 590
NdeII GATC 4 cut(s) 180, 324, 406, 585
NlaIII CATG 6 cut(s) 95, 104, 313, 346, 518, 570
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 569
NspI RCATGY 1 cut(s) 570
PctI GAATGC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 2 cut(s) 52, 140
PkrI GCNGC 5 cut(s) 6, 191, 289, 426, 486
PspPI GGNCC 4 cut(s) 295, 313, 373, 393
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SalI GTCGAC 1 cut(s) 164
SatI GCNGC 5 cut(s) 5, 190, 288, 425, 485
Sau3AI GATC 4 cut(s) 180, 324, 406, 585
Sau96I GGNCC 4 cut(s) 295, 313, 373, 393
ScaI AGTACT 1 cut(s) 527
SduI GDGCHC 1 cut(s) 514
SetI ASST 5 cut(s) 13, 44, 157, 237, 445
SfaNI GCATC 2 cut(s) 112, 605
SinI GGWCC 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 1 cut(s) 133
SsiI CCGC 8 cut(s) 5, 59, 189, 287, 383, 417, 424, 544
TaaI ACNGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 165
TasI AATT 1 cut(s) 133
TatI WGTACW 1 cut(s) 525
TauI GCSGC 4 cut(s) 7, 192, 290, 427
TfiI GAWTC 2 cut(s) 52, 140
TscAI CASTG 2 cut(s) 504, 607
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 1 cut(s) 484
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 4 cut(s) 80, 153, 295, 359
TspRI CASTG 2 cut(s) 504, 607
VpaK11BI GGWCC 1 cut(s) 295
XceI RCATGY 1 cut(s) 570
XmiI GTMKAC 2 cut(s) 165, 278
XmnI GAANNNNTTC 1 cut(s) 458
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.