pycom12g12700

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0022397
Physical Location & Seq
Reverse (-)
15151982 .. 15153369
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12700.2

Sequence Viewer

Length: 987 bp
ATGCCGCGAGGTCGGACCTTCGCTACCATAATACTCACCATCTCTCTCTTGATCCTCGCGGTCTCGGCCAACTTGAGAGATCACATTCTTCATGTCAACCATGTTGACGCAACAAACGCCAATGCTAATCCCAATGATAAATCACAGGAAATAGCCAATGCCGCCGACCACATTGGCAGGGCAGTCAAGGGCATTGCCAACACCGCGAATAGCCTTCTCAATAACCTGGCCACCGCGTTGAAACCAAACACGGACAACTTCACCGAGTACCCTCGACCGGCGCTGGTCCAAGAGTTCCTCATGGCCCATAACGAGATCCGAAGGGAGGAGAAGATGAAGCCGTTGGTGTGGAACGACACGATGGCCCAGTTGGCGGAGGACTGGGCCATGAAGCAGGATCATCACGACTGTCAAATGCAGCATTCATCGTATCAAGGTATGGGGGAGAACTTGTTCTGGGCTTACAAAGCGCATTGGCTGCCGAGGGAGGCAGTGCATGACTGGGGACATGAGAAGGTGTACTACAACAAAGAAAAGCGCGAGTGCGAGCCAGATAAACCATGCGCACATTACACGCAGATTATATGGAAAGACACTGAACAAGTAGGATGCGCCCGACACAAATGCTCCAATGGAAATTTGCTAGTTGTTTGTGAATATTATCCTCCTGGAAATTGGGCCGGCATGGATCCCTTTAACCCAATCCCTTTATCTCAATTAGCTCATCATAACCTGTCGACCACGGCGCCATCACCACCAGCATCGTCTCTTTCTCGGCCGCCACCACCATCATCGCCTAATGGTGAGGCTTCAAATTCAGGTGGCAATCATTTGATGAAACGAAAGGGTAATAAGGGTGGGGGTCATCATGTGCCACGTGGACAAAATGGACAAGGTCAAGGTCAAAAGGGTAATCAAGCCCCAAATAATCACAGACACGGTCAAGGGTCTCATGGTGGCGGTGACGGTCACCATCGTCAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

329

Amino Acids

36.35

Weight (kDa)

8.2

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022397)

Species Orthologous Gene IDs
malus_domestica MD04G1110100.v1.1 MD12G1131300.v1.1
prunus_persica Prupe.6G245700_v2.0.a1
pyrus_communis pycom12g12700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 737
AccII CGCG 5 cut(s) 7, 59, 206, 236, 540
AciI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 779, 960
AclWI GGATC 5 cut(s) 46, 310, 405, 683, 696
AcoI YGGCCR 3 cut(s) 66, 228, 776
AcsI RAATTY 2 cut(s) 637, 814
AcvI CACGTG 1 cut(s) 878
AcyI GRCGYC 1 cut(s) 746
AfaI GTAC 2 cut(s) 269, 521
AfiI CCNNNNNNNGG 3 cut(s) 277, 325, 373
AgsI TTSAA 2 cut(s) 241, 813
AjnI CCWGG 2 cut(s) 225, 667
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 1 cut(s) 722
AluI AGCT 1 cut(s) 722
Alw26I GTCTC 3 cut(s) 67, 771, 954
AlwI GGATC 5 cut(s) 46, 310, 405, 683, 696
AoxI GGCC 7 cut(s) 66, 228, 303, 363, 384, 678, 776
ApeKI GCWGC 2 cut(s) 418, 478
ApoI RAATTY 2 cut(s) 637, 814
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 6 cut(s) 283, 472, 540, 566, 614, 748
AspS9I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 678
AsuHPI GGTGA 6 cut(s) 28, 253, 744, 815, 962, 974
AvaII GGWCC 2 cut(s) 15, 286
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 688
BanI GGYRCC 1 cut(s) 745
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbrPI CACGTG 1 cut(s) 878
BbvI GCAGC 2 cut(s) 430, 465
BccI CCATC 5 cut(s) 47, 355, 757, 796, 981
BceAI ACGGC 2 cut(s) 325, 759
BcgI CGANNNNNNTGC 2 cut(s) 606, 640
BciT130I CCWGG 2 cut(s) 227, 669
BcoDI GTCTC 3 cut(s) 67, 771, 954
BfaI CTAG 2 cut(s) 644, 985
BfoI RGCGCY 2 cut(s) 284, 749
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 5 cut(s) 5, 162, 419, 479, 779
BlsI GCNGC 5 cut(s) 6, 163, 420, 480, 780
Bme1390I CCNGG 2 cut(s) 227, 669
Bme18I GGWCC 2 cut(s) 15, 286
BmgT120I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 678
BmiI GGNNCC 2 cut(s) 690, 747
BmrFI CCNGG 2 cut(s) 227, 669
BmrI ACTGGG 3 cut(s) 361, 391, 511
BmsI GCATC 2 cut(s) 599, 770
BmuI ACTGGG 3 cut(s) 361, 391, 511
BpuEI CTTGAG 1 cut(s) 94
BsaAI YACGTR 1 cut(s) 878
BsaHI GRCGYC 1 cut(s) 746
BsaI GGTCTC 2 cut(s) 67, 954
BsaJI CCNNGG 2 cut(s) 482, 741
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 611, 641
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 325, 373
Bse118I RCCGGY 2 cut(s) 277, 680
Bse1I ACTGG 3 cut(s) 367, 386, 506
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 227, 669
BseDI CCNNGG 2 cut(s) 482, 741
BseGI GGATG 1 cut(s) 614
BseLI CCNNNNNNNGG 3 cut(s) 277, 325, 373
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 3 cut(s) 367, 386, 506
BseRI GAGGAG 1 cut(s) 341
BseX3I CGGCCG 1 cut(s) 776
BseXI GCAGC 2 cut(s) 430, 465
Bsh1236I CGCG 5 cut(s) 7, 59, 206, 236, 540
Bsh1285I CGRYCG 2 cut(s) 278, 779
BshFI GGCC 7 cut(s) 68, 230, 305, 365, 386, 680, 778
BshNI GGYRCC 1 cut(s) 745
BsiEI CGRYCG 2 cut(s) 278, 779
BsiSI CCGG 2 cut(s) 278, 681
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 3 cut(s) 277, 325, 373
BsmAI GTCTC 3 cut(s) 67, 771, 954
BsmBI CGTCTC 1 cut(s) 771
BsmFI GGGAC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 7 cut(s) 68, 230, 305, 365, 386, 680, 778
Bso31I GGTCTC 2 cut(s) 67, 954
Bsp143I GATC 5 cut(s) 51, 79, 315, 397, 688
BspACI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 779, 960
BspANI GGCC 7 cut(s) 68, 230, 305, 365, 386, 680, 778
BspFNI CGCG 5 cut(s) 7, 59, 206, 236, 540
BspLI GGNNCC 2 cut(s) 690, 747
BspPI GGATC 5 cut(s) 46, 310, 405, 683, 696
BspT107I GGYRCC 1 cut(s) 745
BspTNI GGTCTC 2 cut(s) 67, 954
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 277, 680
BsrI ACTGG 3 cut(s) 367, 386, 506
BssAI RCCGGY 2 cut(s) 277, 680
BssECI CCNNGG 2 cut(s) 482, 741
BssMI GATC 5 cut(s) 51, 79, 315, 397, 688
BssNI GRCGYC 1 cut(s) 746
Bst2UI CCWGG 2 cut(s) 227, 669
Bst4CI ACNGT 3 cut(s) 410, 941, 968
BstACI GRCGYC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 478
BstBAI YACGTR 1 cut(s) 878
BstC8I GCNNGC 2 cut(s) 548, 682
BstDSI CCRYGG 1 cut(s) 741
BstEII GGTNACC 1 cut(s) 968
BstF5I GGATG 1 cut(s) 614
BstFNI CGCG 5 cut(s) 7, 59, 206, 236, 540
BstH2I RGCGCY 2 cut(s) 284, 749
BstHHI GCGC 6 cut(s) 283, 472, 540, 566, 614, 748
BstKTI GATC 5 cut(s) 54, 82, 318, 400, 691
BstMAI GTCTC 3 cut(s) 67, 771, 954
BstMBI GATC 5 cut(s) 51, 79, 315, 397, 688
BstMCI CGRYCG 2 cut(s) 278, 779
BstMWI GCNNNNNNNGC 7 cut(s) 65, 116, 161, 203, 371, 467, 478
BstNI CCWGG 2 cut(s) 227, 669
BstPI GGTNACC 1 cut(s) 968
BstSCI CCNGG 2 cut(s) 225, 667
BstUI CGCG 5 cut(s) 7, 59, 206, 236, 540
BstV1I GCAGC 2 cut(s) 430, 465
BstX2I RGATCY 2 cut(s) 315, 688
BstYI RGATCY 2 cut(s) 315, 688
BstZI CGGCCG 1 cut(s) 776
BsuRI GGCC 7 cut(s) 68, 230, 305, 365, 386, 680, 778
BtgI CCRYGG 1 cut(s) 741
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 498, 594
Cac8I GCNNGC 2 cut(s) 548, 682
CfoI GCGC 6 cut(s) 283, 472, 540, 566, 614, 748
Cfr10I RCCGGY 2 cut(s) 277, 680
Cfr13I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 678
CseI GACGC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 268, 520
CviQI GTAC 2 cut(s) 268, 520
DinI GGCGCC 1 cut(s) 747
DpnI GATC 5 cut(s) 53, 81, 317, 399, 690
DpnII GATC 5 cut(s) 51, 79, 315, 397, 688
EaeI YGGCCR 3 cut(s) 66, 228, 776
EagI CGGCCG 1 cut(s) 776
EciI GGCGGA 1 cut(s) 389
EclXI CGGCCG 1 cut(s) 776
Eco31I GGTCTC 2 cut(s) 67, 954
Eco47I GGWCC 2 cut(s) 15, 286
Eco52I CGGCCG 1 cut(s) 776
Eco72I CACGTG 1 cut(s) 878
Eco91I GGTNACC 1 cut(s) 968
EcoO65I GGTNACC 1 cut(s) 968
EcoRII CCWGG 2 cut(s) 225, 667
EgeI GGCGCC 1 cut(s) 747
EheI GGCGCC 1 cut(s) 747
Esp3I CGTCTC 1 cut(s) 771
FaqI GGGAC 1 cut(s) 519
FblI GTMKAC 1 cut(s) 737
Fnu4HI GCNGC 5 cut(s) 5, 162, 419, 479, 779
FokI GGATG 1 cut(s) 621
Fsp4HI GCNGC 5 cut(s) 5, 162, 419, 479, 779
FspAI RTGCGCAY 1 cut(s) 565
FspBI CTAG 2 cut(s) 644, 985
FspI TGCGCA 1 cut(s) 565
GlaI GCGC 6 cut(s) 282, 471, 539, 565, 613, 747
GluI GCNGC 5 cut(s) 5, 162, 419, 479, 779
HaeII RGCGCY 2 cut(s) 284, 749
HaeIII GGCC 7 cut(s) 68, 230, 305, 365, 386, 680, 778
HapII CCGG 2 cut(s) 278, 681
HgaI GACGC 1 cut(s) 116
HhaI GCGC 6 cut(s) 283, 472, 540, 566, 614, 748
Hin1I GRCGYC 1 cut(s) 746
Hin6I GCGC 6 cut(s) 281, 470, 538, 564, 612, 746
HinP1I GCGC 6 cut(s) 281, 470, 538, 564, 612, 746
HincII GTYRAC 4 cut(s) 97, 106, 738, 980
HindII GTYRAC 4 cut(s) 97, 106, 738, 980
HpaII CCGG 2 cut(s) 278, 681
HphI GGTGA 6 cut(s) 28, 253, 744, 815, 962, 974
Hpy166II GTNNAC 6 cut(s) 97, 106, 520, 738, 881, 980
Hpy188I TCNGA 2 cut(s) 15, 320
Hpy188III TCNNGA 2 cut(s) 49, 404
Hpy8I GTNNAC 6 cut(s) 97, 106, 520, 738, 881, 980
HpyAV CCTTC 4 cut(s) 28, 224, 315, 508
HpyCH4III ACNGT 3 cut(s) 410, 941, 968
HpyCH4IV ACGT 1 cut(s) 877
HpyCH4V TGCA 2 cut(s) 418, 496
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 116, 161, 203, 371, 467, 478
HpySE526I ACGT 1 cut(s) 877
Hsp92I GRCGYC 1 cut(s) 746
HspAI GCGC 6 cut(s) 281, 470, 538, 564, 612, 746
KasI GGCGCC 1 cut(s) 745
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
Kzo9I GATC 5 cut(s) 51, 79, 315, 397, 688
LmnI GCTCC 1 cut(s) 632
Lsp1109I GCAGC 2 cut(s) 430, 465
LweI GCATC 2 cut(s) 599, 770
MaeI CTAG 2 cut(s) 644, 985
MaeII ACGT 1 cut(s) 877
MaeIII GTNAC 2 cut(s) 962, 968
MalI GATC 5 cut(s) 53, 81, 317, 399, 690
MboI GATC 5 cut(s) 51, 79, 315, 397, 688
MboII GAAGA 2 cut(s) 80, 343
MflI RGATCY 2 cut(s) 315, 688
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 4 cut(s) 637, 673, 716, 814
MluNI TGGCCA 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 746
MnlI CCTC 9 cut(s) 65, 282, 308, 319, 370, 477, 481, 675, 799
Mox20I TGGCCA 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 230
MseI TTAA 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 2 cut(s) 278, 681
MspR9I CCNGG 2 cut(s) 227, 669
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 2 cut(s) 227, 669
MvnI CGCG 5 cut(s) 7, 59, 206, 236, 540
MwoI GCNNNNNNNGC 7 cut(s) 65, 116, 161, 203, 371, 467, 478
NaeI GCCGGC 1 cut(s) 682
NarI GGCGCC 1 cut(s) 746
NdeII GATC 5 cut(s) 51, 79, 315, 397, 688
NgoMIV GCCGGC 1 cut(s) 680
NlaIV GGNNCC 2 cut(s) 690, 747
NmeAIII GCCGAG 3 cut(s) 44, 507, 754
NmuCI GTSAC 2 cut(s) 962, 968
NsbI TGCGCA 1 cut(s) 565
PctI GAATGC 1 cut(s) 421
PdiI GCCGGC 1 cut(s) 682
PdmI GAANNNNTTC 1 cut(s) 452
PflFI GACNNNGTC 2 cut(s) 894, 939
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 5 cut(s) 6, 163, 420, 480, 780
PluTI GGCGCC 1 cut(s) 749
PmaCI CACGTG 1 cut(s) 878
PmlI CACGTG 1 cut(s) 878
Ppu21I YACGTR 1 cut(s) 878
Psp6I CCWGG 2 cut(s) 225, 667
PspCI CACGTG 1 cut(s) 878
PspEI GGTNACC 1 cut(s) 968
PspGI CCWGG 2 cut(s) 225, 667
PspN4I GGNNCC 2 cut(s) 690, 747
PspPI GGNCC 6 cut(s) 15, 286, 304, 364, 384, 678
PsuI RGATCY 2 cut(s) 315, 688
PsyI GACNNNGTC 2 cut(s) 894, 939
RsaI GTAC 2 cut(s) 269, 521
RsaNI GTAC 2 cut(s) 268, 520
SalI GTCGAC 1 cut(s) 736
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 5 cut(s) 5, 162, 419, 479, 779
Sau3AI GATC 5 cut(s) 51, 79, 315, 397, 688
Sau96I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 678
ScrFI CCNGG 2 cut(s) 227, 669
SfaNI GCATC 2 cut(s) 599, 770
SfoI GGCGCC 1 cut(s) 747
SinI GGWCC 2 cut(s) 15, 286
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 4 cut(s) 637, 673, 716, 814
SsiI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 779, 960
SspDI GGCGCC 1 cut(s) 745
SspI AATATT 1 cut(s) 659
SspMI CTAG 2 cut(s) 644, 985
StyD4I CCNGG 2 cut(s) 225, 667
TaaI ACNGT 3 cut(s) 410, 941, 968
TaiI ACGT 1 cut(s) 880
TaqI TCGA 2 cut(s) 274, 737
TasI AATT 4 cut(s) 637, 673, 716, 814
TatI WGTACW 1 cut(s) 519
TauI GCSGC 3 cut(s) 7, 164, 781
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TscAI CASTG 2 cut(s) 498, 601
TseFI GTSAC 2 cut(s) 962, 968
TseI GCWGC 2 cut(s) 418, 478
Tsp45I GTSAC 2 cut(s) 962, 968
TspDTI ATGAA 5 cut(s) 80, 350, 404, 414, 851
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 2 cut(s) 498, 601
Tth111I GACNNNGTC 2 cut(s) 894, 939
VpaK11BI GGWCC 2 cut(s) 15, 286
XapI RAATTY 2 cut(s) 637, 814
XmiI GTMKAC 1 cut(s) 737
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 2 cut(s) 644, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.