MD12G1131300.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0022397
Physical Location & Seq
Reverse (-)
20646436 .. 20648087
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1131300.v1.1.491

Sequence Viewer

Length: 807 bp
ATGCCGCGAGGTCGGACCTTTGCTACCATAATACTCACCATCTCTCTCCTGATCCTCGCGGTCACGGCCAACTTGAGAGATCACATTCTTCATGTCAACCATGTTGATGGAACAAACGCCAATGCTAATCCCAATGATAGATCACAGGAAATAGCCAATGCCGCCAACCACATTGGCAAGGCTGTCAACGACGTTGCCAACACCGCGAATAGCCTTCTCAATAACCTGGCCACCGCGTTGAAACCAAACACGGACAACTTCACCGAGTACCCTCGACCGGCGCTGGTCCAAGAGTTCCTCCTGGCCCATAACGAGATCCGAAGAGAGGAGAAGATGAAGCCGTTGGTGTGGAACGACACGATGGCCCAGTTGGCGGAGGACTGGGCCATGAAGCAGGATCATCACGACTGTCAAATGCAGCATTCATCGTATCAAGGTATGGGGGAGAACTTGTTCTGGGCTCACAAAGCGCATTGGCTGCCGAGGGAGGCAGTGCAAGACTGGGGACATGAGAAGGTGTACTACAACAAAGAAAAGCGCGAGTGCGAACCAGATAAACCATGCGAACATTACACGCAGATTATATGGAAAGACACTGAACAAGTAGGATGCGCCCGACACAAATGCTCCAATGGAAATTTGCTAGTTGTTTGTGAATATTATCCTCCTGGAAATTGGGTCGGTATGGACCCCTTTAACCCAATCCCTTTATCTGAATTAGCTCATCATAACCTGTCGAGGACGGCGCCATCACCACCAGCATCTTCGCCTTCTCCGCCGCCACCACCATCATCGCCTAATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

269

Amino Acids

30.27

Weight (kDa)

6.19

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 102 - 220 3.1e-23 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022397)

Species Orthologous Gene IDs
malus_domestica MD04G1110100.v1.1 MD12G1131300.v1.1
prunus_persica Prupe.6G245700_v2.0.a1
pyrus_communis pycom12g12700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 745
AccII CGCG 5 cut(s) 7, 59, 206, 236, 540
AciI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 776, 779
AclWI GGATC 3 cut(s) 46, 310, 405
AcoI YGGCCR 2 cut(s) 66, 228
AcsI RAATTY 1 cut(s) 637
AcyI GRCGYC 1 cut(s) 746
AfaI GTAC 2 cut(s) 269, 521
AfiI CCNNNNNNNGG 3 cut(s) 277, 325, 373
AgsI TTSAA 1 cut(s) 241
AjnI CCWGG 3 cut(s) 225, 300, 667
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 1 cut(s) 722
AluI AGCT 1 cut(s) 722
AlwI GGATC 3 cut(s) 46, 310, 405
AoxI GGCC 5 cut(s) 66, 228, 303, 363, 384
ApeKI GCWGC 2 cut(s) 418, 478
ApoI RAATTY 1 cut(s) 637
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 5 cut(s) 283, 472, 540, 614, 748
AspS9I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 688
AsuHPI GGTGA 3 cut(s) 28, 253, 744
AvaII GGWCC 3 cut(s) 15, 286, 688
BalI TGGCCA 1 cut(s) 230
BanI GGYRCC 1 cut(s) 745
BanII GRGCYC 1 cut(s) 463
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbvI GCAGC 2 cut(s) 430, 465
BccI CCATC 5 cut(s) 47, 101, 355, 757, 796
BceAI ACGGC 3 cut(s) 81, 325, 759
BcgI CGANNNNNNTGC 2 cut(s) 606, 640
BciT130I CCWGG 3 cut(s) 227, 302, 669
BfaI CTAG 1 cut(s) 644
BfoI RGCGCY 2 cut(s) 284, 749
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 5 cut(s) 5, 162, 419, 479, 779
BlsI GCNGC 5 cut(s) 6, 163, 420, 480, 780
Bme1390I CCNGG 3 cut(s) 227, 302, 669
Bme18I GGWCC 3 cut(s) 15, 286, 688
BmgT120I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 688
BmiI GGNNCC 2 cut(s) 690, 747
BmrFI CCNGG 3 cut(s) 227, 302, 669
BmrI ACTGGG 3 cut(s) 361, 391, 511
BmsI GCATC 2 cut(s) 599, 770
BmuI ACTGGG 3 cut(s) 361, 391, 511
BpuEI CTTGAG 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 1 cut(s) 482
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 611, 641
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 325, 373
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 3 cut(s) 367, 386, 506
BseBI CCWGG 3 cut(s) 227, 302, 669
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 1 cut(s) 614
BseLI CCNNNNNNNGG 3 cut(s) 277, 325, 373
BseNI ACTGG 3 cut(s) 367, 386, 506
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 430, 465
Bsh1236I CGCG 5 cut(s) 7, 59, 206, 236, 540
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 5 cut(s) 68, 230, 305, 365, 386
BshNI GGYRCC 1 cut(s) 745
BsiEI CGRYCG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 3 cut(s) 277, 325, 373
BsmFI GGGAC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 5 cut(s) 68, 230, 305, 365, 386
Bsp1286I GDGCHC 1 cut(s) 463
Bsp143I GATC 5 cut(s) 51, 79, 140, 315, 397
BspACI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 776, 779
BspANI GGCC 5 cut(s) 68, 230, 305, 365, 386
BspFNI CGCG 5 cut(s) 7, 59, 206, 236, 540
BspLI GGNNCC 2 cut(s) 690, 747
BspPI GGATC 3 cut(s) 46, 310, 405
BspT107I GGYRCC 1 cut(s) 745
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 3 cut(s) 367, 386, 506
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 5 cut(s) 51, 79, 140, 315, 397
BssNI GRCGYC 1 cut(s) 746
Bst2UI CCWGG 3 cut(s) 227, 302, 669
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 478
BstF5I GGATG 1 cut(s) 614
BstFNI CGCG 5 cut(s) 7, 59, 206, 236, 540
BstH2I RGCGCY 2 cut(s) 284, 749
BstHHI GCGC 5 cut(s) 283, 472, 540, 614, 748
BstKTI GATC 5 cut(s) 54, 82, 143, 318, 400
BstMBI GATC 5 cut(s) 51, 79, 140, 315, 397
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 7 cut(s) 65, 161, 203, 371, 467, 478, 775
BstNI CCWGG 3 cut(s) 227, 302, 669
BstSCI CCNGG 3 cut(s) 225, 300, 667
BstUI CGCG 5 cut(s) 7, 59, 206, 236, 540
BstV1I GCAGC 2 cut(s) 430, 465
BstX2I RGATCY 1 cut(s) 315
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 315
BsuRI GGCC 5 cut(s) 68, 230, 305, 365, 386
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 498, 594
CfoI GCGC 5 cut(s) 283, 472, 540, 614, 748
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 688
Csp6I GTAC 2 cut(s) 268, 520
CviAII CATG 5 cut(s) 92, 101, 388, 509, 561
CviQI GTAC 2 cut(s) 268, 520
DinI GGCGCC 1 cut(s) 747
DpnI GATC 5 cut(s) 53, 81, 142, 317, 399
DpnII GATC 5 cut(s) 51, 79, 140, 315, 397
EaeI YGGCCR 2 cut(s) 66, 228
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 2 cut(s) 389, 765
Eco24I GRGCYC 1 cut(s) 463
Eco47I GGWCC 3 cut(s) 15, 286, 688
EcoRII CCWGG 3 cut(s) 225, 300, 667
EcoT38I GRGCYC 1 cut(s) 463
EgeI GGCGCC 1 cut(s) 747
EheI GGCGCC 1 cut(s) 747
FaeI CATG 5 cut(s) 95, 104, 391, 512, 564
FaqI GGGAC 1 cut(s) 519
FatI CATG 5 cut(s) 91, 100, 387, 508, 560
Fnu4HI GCNGC 5 cut(s) 5, 162, 419, 479, 779
FokI GGATG 1 cut(s) 621
FriOI GRGCYC 1 cut(s) 463
Fsp4HI GCNGC 5 cut(s) 5, 162, 419, 479, 779
FspBI CTAG 1 cut(s) 644
GlaI GCGC 5 cut(s) 282, 471, 539, 613, 747
GluI GCNGC 5 cut(s) 5, 162, 419, 479, 779
HaeII RGCGCY 2 cut(s) 284, 749
HaeIII GGCC 5 cut(s) 68, 230, 305, 365, 386
HapII CCGG 1 cut(s) 278
HhaI GCGC 5 cut(s) 283, 472, 540, 614, 748
Hin1I GRCGYC 1 cut(s) 746
Hin1II CATG 5 cut(s) 95, 104, 391, 512, 564
Hin6I GCGC 5 cut(s) 281, 470, 538, 612, 746
HinP1I GCGC 5 cut(s) 281, 470, 538, 612, 746
HincII GTYRAC 2 cut(s) 97, 187
HindII GTYRAC 2 cut(s) 97, 187
HpaII CCGG 1 cut(s) 278
HphI GGTGA 3 cut(s) 28, 253, 744
Hpy166II GTNNAC 3 cut(s) 97, 187, 520
Hpy188I TCNGA 3 cut(s) 15, 320, 715
Hpy188III TCNNGA 2 cut(s) 49, 404
Hpy8I GTNNAC 3 cut(s) 97, 187, 520
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 224, 508, 780
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 418, 496
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 161, 203, 371, 467, 478, 775
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 746
Hsp92II CATG 5 cut(s) 95, 104, 391, 512, 564
HspAI GCGC 5 cut(s) 281, 470, 538, 612, 746
KasI GGCGCC 1 cut(s) 745
Kzo9I GATC 5 cut(s) 51, 79, 140, 315, 397
LmnI GCTCC 1 cut(s) 632
Lsp1109I GCAGC 2 cut(s) 430, 465
LweI GCATC 2 cut(s) 599, 770
MaeI CTAG 1 cut(s) 644
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 61
MalI GATC 5 cut(s) 53, 81, 142, 317, 399
MboI GATC 5 cut(s) 51, 79, 140, 315, 397
MboII GAAGA 4 cut(s) 80, 333, 343, 756
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 463
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 3 cut(s) 637, 673, 716
MluNI TGGCCA 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 746
MnlI CCTC 9 cut(s) 65, 282, 308, 319, 370, 477, 481, 675, 732
Mox20I TGGCCA 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 230
MseI TTAA 1 cut(s) 696
MslI CAYNNNNRTG 1 cut(s) 105
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 3 cut(s) 227, 302, 669
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 3 cut(s) 227, 302, 669
MvnI CGCG 5 cut(s) 7, 59, 206, 236, 540
MwoI GCNNNNNNNGC 7 cut(s) 65, 161, 203, 371, 467, 478, 775
NarI GGCGCC 1 cut(s) 746
NdeII GATC 5 cut(s) 51, 79, 140, 315, 397
NlaIII CATG 5 cut(s) 95, 104, 391, 512, 564
NlaIV GGNNCC 2 cut(s) 690, 747
NmeAIII GCCGAG 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 61
PctI GAATGC 1 cut(s) 421
PdmI GAANNNNTTC 1 cut(s) 452
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 5 cut(s) 6, 163, 420, 480, 780
PluTI GGCGCC 1 cut(s) 749
Psp6I CCWGG 3 cut(s) 225, 300, 667
PspGI CCWGG 3 cut(s) 225, 300, 667
PspN4I GGNNCC 2 cut(s) 690, 747
PspPI GGNCC 6 cut(s) 15, 286, 304, 364, 384, 688
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 2 cut(s) 269, 521
RsaNI GTAC 2 cut(s) 268, 520
RseI CAYNNNNRTG 1 cut(s) 105
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 5 cut(s) 5, 162, 419, 479, 779
Sau3AI GATC 5 cut(s) 51, 79, 140, 315, 397
Sau96I GGNCC 6 cut(s) 15, 286, 304, 364, 384, 688
ScrFI CCNGG 3 cut(s) 227, 302, 669
SduI GDGCHC 1 cut(s) 463
SetI ASST 9 cut(s) 13, 20, 195, 228, 439, 519, 724, 735, 806
SfaNI GCATC 2 cut(s) 599, 770
SfoI GGCGCC 1 cut(s) 747
SinI GGWCC 3 cut(s) 15, 286, 688
SmiMI CAYNNNNRTG 1 cut(s) 105
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 3 cut(s) 637, 673, 716
SsiI CCGC 8 cut(s) 5, 59, 162, 204, 234, 374, 776, 779
SspDI GGCGCC 1 cut(s) 745
SspI AATATT 1 cut(s) 659
SspMI CTAG 1 cut(s) 644
StyD4I CCNGG 3 cut(s) 225, 300, 667
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 274, 737
TasI AATT 3 cut(s) 637, 673, 716
TatI WGTACW 1 cut(s) 519
TauI GCSGC 3 cut(s) 7, 164, 781
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TscAI CASTG 2 cut(s) 498, 601
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 2 cut(s) 418, 478
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 80, 350, 404, 414
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 2 cut(s) 498, 601
VpaK11BI GGWCC 3 cut(s) 15, 286, 688
XapI RAATTY 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.