MD04G1139100.v1.1

Protease inhibitor seed storage lipid transfer family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014001
Physical Location & Seq
Reverse (-)
22744433 .. 22745628
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1139100.v1.1.491

Sequence Viewer

Length: 357 bp
ATGGCAGCTGCAGTGAAATTTATTTGCGTGTTAGGGTTTCTTGCAGTTTTTAGCATTGCTATGATTGAAAGGGTTGATGGGGCAAGTGCTTGTGGAAAAGCTAGTCCAGACGAAGAGGCCGCCAAGCTTGCTCCTTGTGCAGATGCCGCACAAGATGAAAAGGCTGCTGTTTCCGCTAGTTGCTGTCAGATAGTCAAGAGGATGGGACAAAACACTAGCTGCCTCTGTGCCATCCTGCTTTCTGACGCCGCCAAGAGCTCTGGCGTCAAGCTTGAGGTTGCGATAACCATTCCTAAGCGCTGCAACTTAGCCAACCGCCCAGTCGGTTACAAGTGCGGAGGCTATACCCTGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

119

Amino Acids

12.13

Weight (kDa)

8.39

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 36 - 108 2.9e-10 Probable lipid transfer
Tryp_alpha_amyl PF00234 41 - 112 1.4e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53980 AT3G53980
fragaria_vesca FvH4_6g12710
malus_domestica MD04G1139100.v1.1 MD12G1154900.v1.1
prunus_persica Prupe.6G264700_v2.0.a1
pyrus_communis pycom04g12740
rosa_chinensis RchiOBHm_Chr3g0464631
rosa_laevigata RLG00000024679
rosa_multiflora Rmu_sc0002031.1_g000007 Rmu_sc0014550.1_g000013
rosa_roxburghii Rroxscaffold_6G00415520
rosa_rugosa Rorug03G0073300.1
rosa_samantha Rh3BG135600 Rh3CG138200 Rh3DG137200
rosa_wichuraiana Rw3G011100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 120, 147, 174, 249, 316, 336
AcsI RAATTY 1 cut(s) 17
AcyI GRCGYC 2 cut(s) 246, 264
AfeI AGCGCT 1 cut(s) 299
AgsI TTSAA 1 cut(s) 68
AluBI AGCT 6 cut(s) 8, 101, 127, 219, 258, 271
AluI AGCT 6 cut(s) 8, 101, 127, 219, 258, 271
Alw21I GWGCWC 1 cut(s) 260
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 5 cut(s) 5, 8, 164, 219, 300
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 1 cut(s) 300
BanII GRGCYC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 17, 151, 206, 287
BccI CCATC 3 cut(s) 71, 196, 239
BceAI ACGGC 1 cut(s) 337
BfaI CTAG 3 cut(s) 102, 177, 216
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 8 cut(s) 6, 9, 120, 147, 165, 220, 249, 301
BlsI GCNGC 8 cut(s) 7, 10, 121, 148, 166, 221, 250, 302
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 314
Bpu10I CCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 293
BsaHI GRCGYC 2 cut(s) 246, 264
Bse1I ACTGG 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 54
BseGI GGATG 2 cut(s) 207, 231
BseMI GCAATG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 320
BseXI GCAGC 4 cut(s) 17, 151, 206, 287
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 260
BslFI GGGAC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 260
BspACI CCGC 6 cut(s) 120, 147, 174, 249, 316, 336
BspANI GGCC 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 54
BsrI ACTGG 1 cut(s) 320
BssNI GRCGYC 2 cut(s) 246, 264
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 2 cut(s) 246, 264
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 294, 307
BstF5I GGATG 2 cut(s) 207, 231
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstMWI GCNNNNNNNGC 4 cut(s) 128, 137, 146, 173
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 4 cut(s) 17, 151, 206, 287
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 207, 231
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 300
CseI GACGC 2 cut(s) 253, 254
DdeI CTNAG 2 cut(s) 294, 307
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 260
Eco47III AGCGCT 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 260
FaiI YATR 2 cut(s) 62, 345
FaqI GGGAC 1 cut(s) 219
Fnu4HI GCNGC 8 cut(s) 6, 9, 120, 147, 165, 220, 249, 301
FokI GGATG 2 cut(s) 214, 218
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 8 cut(s) 6, 9, 120, 147, 165, 220, 249, 301
FspBI CTAG 3 cut(s) 102, 177, 216
GlaI GCGC 1 cut(s) 299
GluI GCNGC 8 cut(s) 6, 9, 120, 147, 165, 220, 249, 301
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 1 cut(s) 119
HgaI GACGC 2 cut(s) 253, 254
HhaI GCGC 1 cut(s) 300
Hin1I GRCGYC 2 cut(s) 246, 264
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HindIII AAGCTT 2 cut(s) 125, 269
Hpy188I TCNGA 2 cut(s) 189, 244
Hpy188III TCNNGA 2 cut(s) 107, 196
HpyCH4V TGCA 4 cut(s) 11, 44, 140, 303
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 137, 146, 173
HpyF3I CTNAG 2 cut(s) 294, 307
Hsp92I GRCGYC 2 cut(s) 246, 264
HspAI GCGC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 4 cut(s) 120, 246, 248, 333
Lsp1109I GCAGC 4 cut(s) 17, 151, 206, 287
LweI GCATC 1 cut(s) 133
MaeI CTAG 3 cut(s) 102, 177, 216
MaeIII GTNAC 1 cut(s) 326
MboII GAAGA 1 cut(s) 125
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 1 cut(s) 17
MnlI CCTC 5 cut(s) 109, 192, 233, 268, 332
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 8
MwoI GCNNNNNNNGC 4 cut(s) 128, 137, 146, 173
PkrI GCNGC 8 cut(s) 7, 10, 121, 148, 166, 221, 250, 302
Psp124BI GAGCTC 1 cut(s) 260
PstI CTGCAG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 8
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 260
SatI GCNGC 8 cut(s) 6, 9, 120, 147, 165, 220, 249, 301
SduI GDGCHC 1 cut(s) 260
SetI ASST 7 cut(s) 10, 103, 129, 221, 260, 273, 279
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 17
SsiI CCGC 6 cut(s) 120, 147, 174, 249, 316, 336
SspMI CTAG 3 cut(s) 102, 177, 216
SstI GAGCTC 1 cut(s) 260
TasI AATT 1 cut(s) 17
TauI GCSGC 3 cut(s) 122, 149, 251
TscAI CASTG 1 cut(s) 18
TseI GCWGC 5 cut(s) 5, 8, 164, 219, 300
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 1 cut(s) 18
XapI RAATTY 1 cut(s) 17
XspI CTAG 3 cut(s) 102, 177, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.