MD04G1148100.v1.1

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0020862
Physical Location & Seq
Reverse (-)
23659400 .. 23662131
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1148100.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGTAAGGCTTCAAGGGATAAGAGGGATATCTACTACCGGAAAGCAAAGGAAGAAGGATGGCGTGCACGCAGCGCCTTCAAGCTCCTCCAGATCGACGAGGAGTTCAATATTTTCGACGGCGTCAAGCGTGTCGTCGATCTCTGTGCCGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAATTGTATCTCCCAGCAAAAAGTTCATCTGATTCTAAGGATGGCGGTGTGCCACTTATTGTAGCTATTGATTTGCAGCCAATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACTAATGCCCGAACTGCGGAAGTGGTCATCAGACATTTCGACGGCTGCAAAGCTGACCTGGTTGTGTGTGATGGTGCTCCAGACGTTACTGGCCTTCATGACATGGATGAATTTGTCCAGTCCCAGCTGATACTAGCAGGTTTAACAATTGTGACTCATGTACTGAAAGAAGGTGGTAAATTTATAGCAAAGATATTCCGTGGGAAAGATACAAGTCTTCTGTACTGTCAGCTGAAATTATTTTTCCCTGTCGTGACTTTTGCAAAGCCGAAAAGCAGCCGCAACTCCAGCATAGAGGCTTTTGCAGTTTGTGAGAATTATTCCCCTCCCGATGAGTTCAACCCTAAGGATCTGCATCGCCTGCTAGAAAAAGTGGGAAGCCCCTCCGGAGCAGATGATCTAGATTGCACTAGCGGGTGGTTGGAAGGGCCAAATAAGGTGTACATTCCATTTTTAGCATGCGGTGATCTGAGTGGGTATGACTCCGACCGCTCCTACCCTCTGCCTAAAGACGCCCAGGGAACTTACCGGAGCTTGGATCCCGTACAGCCCCCAATCGCCCCTCCTTATAAGAGAGCTCTGGAAATGAAGAAAGCTTCCAGCCAGGGCATTACAGAGCATGAGAAGCTTTCGTTGGAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.8

Weight (kDa)

6.02

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 209 1e-50 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020862)

Species Orthologous Gene IDs
malus_domestica MD04G1148100.v1.1 MD12G1161600.v1.1
prunus_persica Prupe.6G272000_v2.0.a1
pyrus_communis pycom04g13400 pycom12g15340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 798
AccIII TCCGGA 1 cut(s) 692
AciI CCGC 7 cut(s) 150, 228, 323, 586, 720, 768, 796
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 2 cut(s) 416, 485
AcyI GRCGYC 2 cut(s) 123, 819
AfaI GTAC 4 cut(s) 468, 530, 749, 852
AfiI CCNNNNNNNGG 3 cut(s) 278, 322, 841
AgsI TTSAA 6 cut(s) 15, 82, 109, 278, 290, 646
AjnI CCWGG 4 cut(s) 154, 363, 822, 909
AjuI GAANNNNNNNTTGG 1 cut(s) 923
Alw21I GWGCWC 3 cut(s) 70, 385, 886
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 3 cut(s) 663, 839, 852
Aor13HI TCCGGA 1 cut(s) 692
AoxI GGCC 2 cut(s) 397, 734
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 72, 259, 351, 582
ApoI RAATTY 2 cut(s) 416, 485
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 2 cut(s) 311, 782
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 70
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvI GCAGC 4 cut(s) 84, 271, 338, 594
BccI CCATC 3 cut(s) 54, 218, 371
BceAI ACGGC 2 cut(s) 136, 364
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 4 cut(s) 156, 365, 824, 911
BfaI CTAG 4 cut(s) 440, 671, 707, 717
BfoI RGCGCY 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 6 cut(s) 73, 150, 260, 352, 583, 586
BlsI GCNGC 6 cut(s) 74, 151, 261, 353, 584, 587
Bme1390I CCNGG 4 cut(s) 156, 365, 824, 911
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 4 cut(s) 156, 365, 824, 911
BmsI GCATC 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 4 cut(s) 74, 184, 369, 577
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 2 cut(s) 123, 819
BsaJI CCNNGG 5 cut(s) 154, 505, 822, 823, 910
BsaWI WCCGGW 3 cut(s) 39, 692, 834
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 322, 841
Bse1I ACTGG 2 cut(s) 400, 424
Bse21I CCTNAGG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 692
BseBI CCWGG 4 cut(s) 156, 365, 824, 911
BseDI CCNNGG 5 cut(s) 154, 505, 822, 823, 910
BseGI GGATG 3 cut(s) 65, 229, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 278, 322, 841
BseMII CTCAG 1 cut(s) 767
BseNI ACTGG 2 cut(s) 400, 424
BseRI GAGGAG 2 cut(s) 77, 116
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 4 cut(s) 84, 271, 338, 594
BseYI CCCAGC 2 cut(s) 196, 429
BsgI GTGCAG 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 796
BshFI GGCC 2 cut(s) 399, 736
BsiEI CGRYCG 1 cut(s) 796
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BsiSI CCGG 3 cut(s) 40, 693, 835
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 3 cut(s) 278, 322, 841
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 2 cut(s) 399, 736
Bsp1286I GDGCHC 3 cut(s) 70, 385, 886
Bsp13I TCCGGA 1 cut(s) 692
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 6 cut(s) 93, 139, 655, 703, 772, 844
BspACI CCGC 7 cut(s) 150, 228, 323, 586, 720, 768, 796
BspANI GGCC 2 cut(s) 399, 736
BspCNI CTCAG 1 cut(s) 768
BspEI TCCGGA 1 cut(s) 692
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 663, 839, 852
BsrBI CCGCTC 1 cut(s) 798
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 2 cut(s) 400, 424
BssECI CCNNGG 5 cut(s) 154, 505, 822, 823, 910
BssMI GATC 6 cut(s) 93, 139, 655, 703, 772, 844
BssNI GRCGYC 2 cut(s) 123, 819
Bst2UI CCWGG 4 cut(s) 156, 365, 824, 911
Bst4CI ACNGT 1 cut(s) 533
BstACI GRCGYC 2 cut(s) 123, 819
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 747
BstC8I GCNNGC 4 cut(s) 66, 70, 668, 766
BstDEI CTNAG 3 cut(s) 219, 651, 776
BstDSI CCRYGG 1 cut(s) 505
BstF5I GGATG 3 cut(s) 65, 229, 418
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 6 cut(s) 96, 142, 658, 706, 775, 847
BstMBI GATC 6 cut(s) 93, 139, 655, 703, 772, 844
BstMCI CGRYCG 1 cut(s) 796
BstMWI GCNNNNNNNGC 5 cut(s) 74, 320, 594, 667, 931
BstNI CCWGG 4 cut(s) 156, 365, 824, 911
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 4 cut(s) 154, 363, 822, 909
BstSLI GKGCMC 1 cut(s) 70
BstV1I GCAGC 4 cut(s) 84, 271, 338, 594
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 2 cut(s) 399, 736
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 1 cut(s) 647
BtsCI GGATG 3 cut(s) 65, 229, 418
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 66, 70, 668, 766
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 734
CseI GACGC 2 cut(s) 112, 827
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 4 cut(s) 467, 529, 748, 851
CviAII CATG 5 cut(s) 404, 409, 464, 765, 926
CviQI GTAC 4 cut(s) 467, 529, 748, 851
DdeI CTNAG 3 cut(s) 219, 651, 776
DpnI GATC 6 cut(s) 95, 141, 657, 705, 774, 846
DpnII GATC 6 cut(s) 93, 139, 655, 703, 772, 844
Ecl136II GAGCTC 1 cut(s) 884
Eco24I GRGCYC 1 cut(s) 886
Eco32I GATATC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 884
Eco81I CCTNAGG 1 cut(s) 651
EcoICRI GAGCTC 1 cut(s) 884
EcoRII CCWGG 4 cut(s) 154, 363, 822, 909
EcoRV GATATC 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 886
FaeI CATG 5 cut(s) 407, 412, 467, 768, 929
FaqI GGGAC 1 cut(s) 412
FatI CATG 5 cut(s) 403, 408, 463, 764, 925
FauI CCCGC 1 cut(s) 713
Fnu4HI GCNGC 6 cut(s) 73, 150, 260, 352, 583, 586
FokI GGATG 3 cut(s) 72, 236, 425
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 6 cut(s) 73, 150, 260, 352, 583, 586
FspBI CTAG 4 cut(s) 440, 671, 707, 717
GlaI GCGC 1 cut(s) 76
GluI GCNGC 6 cut(s) 73, 150, 260, 352, 583, 586
GsaI CCCAGC 2 cut(s) 200, 433
GsuI CTGGAG 4 cut(s) 74, 184, 369, 577
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 399, 736
HapII CCGG 3 cut(s) 40, 693, 835
HgaI GACGC 2 cut(s) 112, 827
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 2 cut(s) 123, 819
Hin1II CATG 5 cut(s) 407, 412, 467, 768, 929
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HindIII AAGCTT 2 cut(s) 900, 932
HinfI GANTC 5 cut(s) 166, 215, 460, 788, 944
HpaII CCGG 3 cut(s) 40, 693, 835
HphI GGTGA 2 cut(s) 311, 782
Hpy166II GTNNAC 2 cut(s) 68, 748
Hpy188I TCNGA 4 cut(s) 214, 338, 777, 793
Hpy188III TCNNGA 9 cut(s) 91, 386, 404, 559, 635, 693, 707, 887, 948
Hpy8I GTNNAC 2 cut(s) 68, 748
Hpy99I CGWCG 4 cut(s) 101, 122, 140, 350
HpyAV CCTTC 6 cut(s) 50, 88, 272, 410, 470, 725
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 8 cut(s) 68, 259, 295, 354, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 320, 594, 667, 931
HpyF3I CTNAG 3 cut(s) 219, 651, 776
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 2 cut(s) 123, 819
Hsp92II CATG 5 cut(s) 407, 412, 467, 768, 929
HspAI GCGC 1 cut(s) 75
Kpn2I TCCGGA 1 cut(s) 692
Kzo9I GATC 6 cut(s) 93, 139, 655, 703, 772, 844
LmnI GCTCC 6 cut(s) 90, 274, 388, 695, 803, 837
Lsp1109I GCAGC 4 cut(s) 84, 271, 338, 594
LweI GCATC 1 cut(s) 670
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 4 cut(s) 440, 671, 707, 717
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 3 cut(s) 391, 457, 559
MalI GATC 6 cut(s) 95, 141, 657, 705, 774, 846
MbiI CCGCTC 1 cut(s) 798
MboI GATC 6 cut(s) 93, 139, 655, 703, 772, 844
MboII GAAGA 3 cut(s) 65, 515, 907
MfeI CAATTG 2 cut(s) 273, 453
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 3 cut(s) 70, 385, 886
MluCI AATT 7 cut(s) 185, 273, 416, 453, 485, 542, 622
MlyI GAGTC 3 cut(s) 175, 454, 782
MmeI TCCRAC 3 cut(s) 708, 816, 921
MnlI CCTC 8 cut(s) 18, 94, 98, 595, 642, 700, 816, 879
MroI TCCGGA 1 cut(s) 692
MseI TTAA 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 433, 538
MspI CCGG 3 cut(s) 40, 693, 835
MspR9I CCNGG 4 cut(s) 156, 365, 824, 911
MunI CAATTG 2 cut(s) 273, 453
MvaI CCWGG 4 cut(s) 156, 365, 824, 911
MwoI GCNNNNNNNGC 5 cut(s) 74, 320, 594, 667, 931
NdeII GATC 6 cut(s) 93, 139, 655, 703, 772, 844
NlaIII CATG 5 cut(s) 407, 412, 467, 768, 929
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 2 cut(s) 457, 559
NspI RCATGY 1 cut(s) 768
PaeI GCATGC 1 cut(s) 768
PagI TCATGA 1 cut(s) 403
PasI CCCWGGG 1 cut(s) 823
PfeI GAWTC 2 cut(s) 215, 944
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 6 cut(s) 74, 151, 261, 353, 584, 587
PleI GAGTC 3 cut(s) 174, 454, 782
PpsI GAGTC 3 cut(s) 174, 454, 782
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 4 cut(s) 154, 363, 822, 909
PspFI CCCAGC 2 cut(s) 196, 429
PspGI CCWGG 4 cut(s) 154, 363, 822, 909
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 2 cut(s) 655, 844
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 2 cut(s) 433, 538
RsaI GTAC 4 cut(s) 468, 530, 749, 852
RsaNI GTAC 4 cut(s) 467, 529, 748, 851
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 6 cut(s) 73, 150, 260, 352, 583, 586
Sau3AI GATC 6 cut(s) 93, 139, 655, 703, 772, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 3 cut(s) 175, 454, 782
ScrFI CCNGG 4 cut(s) 156, 365, 824, 911
SduI GDGCHC 3 cut(s) 70, 385, 886
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 1 cut(s) 670
SphI GCATGC 1 cut(s) 768
Sse9I AATT 7 cut(s) 185, 273, 416, 453, 485, 542, 622
SsiI CCGC 7 cut(s) 150, 228, 323, 586, 720, 768, 796
SspI AATATT 1 cut(s) 112
SspMI CTAG 4 cut(s) 440, 671, 707, 717
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 154, 363, 822, 909
TaaI ACNGT 1 cut(s) 533
TaiI ACGT 1 cut(s) 393
TaqI TCGA 4 cut(s) 96, 117, 138, 345
TasI AATT 7 cut(s) 185, 273, 416, 453, 485, 542, 622
TatI WGTACW 3 cut(s) 466, 528, 747
TauI GCSGC 2 cut(s) 152, 588
TfiI GAWTC 2 cut(s) 215, 944
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TseFI GTSAC 2 cut(s) 457, 559
TseI GCWGC 4 cut(s) 72, 259, 351, 582
Tsp45I GTSAC 2 cut(s) 457, 559
TspDTI ATGAA 4 cut(s) 198, 392, 429, 908
TspGWI ACGGA 1 cut(s) 494
Tth111I GACNNNGTC 1 cut(s) 122
VneI GTGCAC 1 cut(s) 66
XapI RAATTY 2 cut(s) 416, 485
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 1 cut(s) 768
XspI CTAG 4 cut(s) 440, 671, 707, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.