Prupe.6G272000_v2.0.a1

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0020862
Physical Location & Seq
Reverse (-)
25760218 .. 25763028
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G272000.1

Sequence Viewer

Length: 951 bp
ATGGGGAAAGCTTCAAGGGATAAGAGGGATATATACTATCGAAAAGCAAAAGAAGAAGGTTGGCGTGCTCGCAGTGCCTTCAAGCTTCTTCAGATTGACGAGGAATTCAACATATTTGACGGAGTGAAGCGGGTCGTCGATCTTTGTGCTGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAATTGTATCTCCCAGCAAAGAGTTCATCAGATTCTAAGGATGGTGATGTGCCCCTTATTGTAGCTATTGATTTGCAGCCCATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACTAATGCTCGAACCGCTGAAATCGTCATCAGACATTTTGATGGTTGCAAGGCTGACCTGGTTGTGTGTGATGGTGCTCCAGATGTTACTGGCCTTCATGACATGGATGAATTCGTTCAGTCCCAGCTGATACTAGCAGGTTTGACGATTGTTACTCATGTACTTAAAGAAGGTGGAAAATTTATTGCAAAGATATTCCGTGGAAAAGATACAAGTCTTCTCTACTGCCAGCTGAAACTATTCTTCCCCTTAGTGACTTTTGCAAAACCAAAAAGTAGTCGCAATTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCTGATGGATTCAACCCTAAGGATCTGCATCGTCTCCTAGAAAAGGTGGGAAGTCCCTCAGGAGCAGATGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGGCCAAATAAGGTGTATATTCCATTTCTAGCTTGCGGTGACCTCAGCGGATATGACTCTGACCGTTCATATCCCCTACCTAAAGATGCCCAGGGAACTTACCGGAGCTTGGATCCCATACAGCCCCCAATAGCCCCTCCTTATAAGAGAGCTCTAGAAATGAAGAAAGCTTCCAGCCAGGGCATCACAGAGCTTGAGAAGCCTTCTTTGGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.78

Weight (kDa)

5.91

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020862)

Species Orthologous Gene IDs
malus_domestica MD04G1148100.v1.1 MD12G1161600.v1.1
prunus_persica Prupe.6G272000_v2.0.a1
pyrus_communis pycom04g13400 pycom12g15340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 434
AciI CCGC 4 cut(s) 130, 321, 768, 780
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 3 cut(s) 104, 416, 485
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
AgsI TTSAA 6 cut(s) 15, 82, 109, 278, 290, 646
AjnI CCWGG 4 cut(s) 154, 363, 822, 909
AjuI GAANNNNNNNTTGG 1 cut(s) 923
Alw21I GWGCWC 3 cut(s) 70, 385, 886
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 3 cut(s) 663, 839, 852
AoxI GGCC 2 cut(s) 397, 734
ApeKI GCWGC 2 cut(s) 149, 259
ApoI RAATTY 3 cut(s) 104, 416, 485
Asp700I GAANNNNTTC 2 cut(s) 420, 545
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 3 cut(s) 239, 311, 782
AxyI CCTNAGG 2 cut(s) 651, 691
BaeGI GKGCMC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvCI CCTCAGC 1 cut(s) 776
BbvI GCAGC 2 cut(s) 136, 271
BccI CCATC 4 cut(s) 218, 341, 371, 632
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 4 cut(s) 156, 365, 824, 911
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 5 cut(s) 440, 671, 707, 761, 887
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 2 cut(s) 150, 260
BlsI GCNGC 2 cut(s) 151, 261
Bme1390I CCNGG 4 cut(s) 156, 365, 824, 911
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 4 cut(s) 156, 365, 824, 911
BmsI GCATC 3 cut(s) 670, 808, 924
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 2 cut(s) 184, 369
Bpu10I CCTNAGC 1 cut(s) 776
BpuEI CTTGAG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BsaWI WCCGGW 1 cut(s) 834
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 2 cut(s) 651, 691
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 4 cut(s) 156, 365, 824, 911
BseDI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BseGI GGATG 2 cut(s) 229, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
BseMII CTCAG 2 cut(s) 705, 790
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 136, 271
BseYI CCCAGC 2 cut(s) 196, 429
BsgI GTGCAG 1 cut(s) 314
BshFI GGCC 2 cut(s) 399, 736
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BsiSI CCGG 1 cut(s) 835
BslFI GGGAC 2 cut(s) 412, 672
BslI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
BsmAI GTCTC 1 cut(s) 671
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 2 cut(s) 412, 672
BsnI GGCC 2 cut(s) 399, 736
Bsp1286I GDGCHC 4 cut(s) 70, 237, 385, 886
Bsp143I GATC 4 cut(s) 139, 655, 703, 844
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 4 cut(s) 130, 321, 768, 780
BspANI GGCC 2 cut(s) 399, 736
BspCNI CTCAG 2 cut(s) 704, 789
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 663, 839, 852
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BssMI GATC 4 cut(s) 139, 655, 703, 844
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 4 cut(s) 156, 365, 824, 911
Bst4CI ACNGT 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 66, 70, 536, 766
BstDEI CTNAG 5 cut(s) 219, 556, 651, 691, 776
BstDSI CCRYGG 2 cut(s) 264, 505
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 2 cut(s) 229, 418
BstKTI GATC 4 cut(s) 142, 658, 706, 847
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 4 cut(s) 139, 655, 703, 844
BstMWI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 931
BstNI CCWGG 4 cut(s) 156, 365, 824, 911
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 4 cut(s) 154, 363, 822, 909
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 136, 271
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 2 cut(s) 651, 691
BsuRI GGCC 2 cut(s) 399, 736
BtgI CCRYGG 2 cut(s) 264, 505
BtsCI GGATG 2 cut(s) 229, 418
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 66, 70, 536, 766
CciI TCATGA 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 734
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 1 cut(s) 467
CviAII CATG 4 cut(s) 265, 404, 409, 464
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 5 cut(s) 219, 556, 651, 691, 776
DpnI GATC 4 cut(s) 141, 657, 705, 846
DpnII GATC 4 cut(s) 139, 655, 703, 844
Ecl136II GAGCTC 1 cut(s) 884
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 74
Eco81I CCTNAGG 2 cut(s) 651, 691
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 884
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 2 cut(s) 104, 416
EcoRII CCWGG 4 cut(s) 154, 363, 822, 909
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 1 cut(s) 264
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 4 cut(s) 268, 407, 412, 467
FaqI GGGAC 2 cut(s) 412, 672
FatI CATG 4 cut(s) 264, 403, 408, 463
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 150, 260
FokI GGATG 2 cut(s) 236, 425
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 2 cut(s) 150, 260
FspBI CTAG 5 cut(s) 440, 671, 707, 761, 887
GluI GCNGC 2 cut(s) 150, 260
GsaI CCCAGC 2 cut(s) 200, 433
GsuI CTGGAG 2 cut(s) 184, 369
HaeIII GGCC 2 cut(s) 399, 736
HapII CCGG 1 cut(s) 835
Hin1II CATG 4 cut(s) 268, 407, 412, 467
HindIII AAGCTT 3 cut(s) 9, 83, 900
HinfI GANTC 5 cut(s) 166, 215, 642, 788, 944
HpaII CCGG 1 cut(s) 835
HphI GGTGA 3 cut(s) 239, 311, 782
Hpy188I TCNGA 4 cut(s) 93, 214, 338, 793
Hpy188III TCNNGA 6 cut(s) 386, 404, 635, 693, 707, 887
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 7 cut(s) 50, 88, 272, 410, 470, 725, 945
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4V TGCA 8 cut(s) 259, 295, 354, 494, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 931
HpyF3I CTNAG 5 cut(s) 219, 556, 651, 691, 776
Hsp92II CATG 4 cut(s) 268, 407, 412, 467
Kzo9I GATC 4 cut(s) 139, 655, 703, 844
LmnI GCTCC 4 cut(s) 274, 388, 695, 837
Lsp1109I GCAGC 2 cut(s) 136, 271
LweI GCATC 3 cut(s) 670, 808, 924
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 5 cut(s) 440, 671, 707, 761, 887
MaeIII GTNAC 4 cut(s) 391, 457, 559, 770
MalI GATC 4 cut(s) 141, 657, 705, 846
MboI GATC 4 cut(s) 139, 655, 703, 844
MboII GAAGA 5 cut(s) 65, 80, 515, 541, 907
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 4 cut(s) 70, 237, 385, 886
MluCI AATT 7 cut(s) 104, 185, 273, 416, 485, 589, 622
MlyI GAGTC 2 cut(s) 175, 782
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 7 cut(s) 18, 94, 595, 642, 700, 785, 879
MroXI GAANNNNTTC 2 cut(s) 420, 545
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 4 cut(s) 323, 433, 538, 780
MspI CCGG 1 cut(s) 835
MspR9I CCNGG 4 cut(s) 156, 365, 824, 911
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 4 cut(s) 156, 365, 824, 911
MwoI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 931
NcoI CCATGG 1 cut(s) 264
NdeII GATC 4 cut(s) 139, 655, 703, 844
NlaIII CATG 4 cut(s) 268, 407, 412, 467
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 2 cut(s) 559, 770
PagI TCATGA 1 cut(s) 403
PasI CCCWGGG 1 cut(s) 823
PdmI GAANNNNTTC 2 cut(s) 420, 545
PfeI GAWTC 2 cut(s) 215, 642
PkrI GCNGC 2 cut(s) 151, 261
PleI GAGTC 2 cut(s) 174, 782
PpsI GAGTC 2 cut(s) 174, 782
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 4 cut(s) 154, 363, 822, 909
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 196, 429
PspGI CCWGG 4 cut(s) 154, 363, 822, 909
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 2 cut(s) 655, 844
PvuII CAGCTG 2 cut(s) 433, 538
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 2 cut(s) 150, 260
Sau3AI GATC 4 cut(s) 139, 655, 703, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 2 cut(s) 175, 782
ScrFI CCNGG 4 cut(s) 156, 365, 824, 911
SduI GDGCHC 4 cut(s) 70, 237, 385, 886
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 3 cut(s) 670, 808, 924
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 7 cut(s) 104, 185, 273, 416, 485, 589, 622
SsiI CCGC 4 cut(s) 130, 321, 768, 780
SspMI CTAG 5 cut(s) 440, 671, 707, 761, 887
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 154, 363, 822, 909
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 797
TaqI TCGA 3 cut(s) 40, 138, 316
TasI AATT 7 cut(s) 104, 185, 273, 416, 485, 589, 622
TatI WGTACW 1 cut(s) 466
TfiI GAWTC 2 cut(s) 215, 642
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 559, 770
TseI GCWGC 2 cut(s) 149, 259
Tsp45I GTSAC 2 cut(s) 559, 770
TspDTI ATGAA 5 cut(s) 198, 392, 429, 789, 908
TspGWI ACGGA 2 cut(s) 135, 494
TspRI CASTG 1 cut(s) 79
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 3 cut(s) 104, 416, 485
XbaI TCTAGA 2 cut(s) 706, 886
XmnI GAANNNNTTC 2 cut(s) 420, 545
XspI CTAG 5 cut(s) 440, 671, 707, 761, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.