MD12G1161600.v1.1

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0020862
Physical Location & Seq
Reverse (-)
24280859 .. 24283646
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1161600.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGGAAAGCTTCAAGGGATAAGCGGGATATCTACTACCGGAAAGCAAAAGAAGAAGGATGGCGTGCACGCAGCGCCTTCAAGCTCCTCCAGATCGACGAGGAGTTCAATATTTTCGACGGCGTCAAGCGGGTCGTCGATCTCTGTGCCGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAATTGTATCTCCCAGCAAAGAGTTCATCTGATTCTAAAGATGGCGATGTTCCGCTTATCGTAGCTATTGATTTGCAGCCAATGGCTCCGATTGAAGGTGTTATTCAAGTCCAGGGTGATATAACTAATGCCCGAACCGCTGAAGTGGTCATCAGACATTTCGACGGTTGCAAAGCTGACCTGGTTGTGTGTGATGGTGCTCCAGATGTAACTGGCCTTCATGACATGGATGAATTTGTTCAGTCCCAGCTAATACTAGCGGGTTTAACAATTGTGACTCATGTACTTAAAGAGGGTGGTAAATTTATAGCAAAGATATTCCGTGGAAAAGATACAAGTCTTCTGTACTGTCAGCTGAAACTATTTTTCCCCATCGTGACTTTTGCAAAGCCGAAAAGCAGCCGCAACTCCAGCATAGAGGCATTTGCAGTCTGTGAGAATTATTCCCCTCCTGATGGGTTCAACCCTAAGGATCTGCATCGCCTGCTAGAAAAGGTGGGAAGCCCCTCCGGAGCAGATGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGGCCCAATAAGGTGTACATTCCATTTTTAGCATGTGGTGACCTCAATGGGTATGACTCCGACCGCTCCTATCCGCTGCCTAAAGACGCCCAGGGAAGTTACCGGAGCTTGGATCCTGTACAGCCCCCAATTGCCCCTCCATATAAGAGAGCTCTAGAAATGAAGAAAGCTTCCAGCCAGGGCATCACAGAGCTTGAGAAACTTTCGTTGGAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.78

Weight (kDa)

5.91

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 209 4.9e-50 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020862)

Species Orthologous Gene IDs
malus_domestica MD04G1148100.v1.1 MD12G1161600.v1.1
prunus_persica Prupe.6G272000_v2.0.a1
pyrus_communis pycom04g13400 pycom12g15340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 798
AccIII TCCGGA 1 cut(s) 692
AciI CCGC 9 cut(s) 25, 130, 150, 236, 321, 443, 586, 796, 806
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 2 cut(s) 416, 485
AcuI CTGAAG 1 cut(s) 345
AcyI GRCGYC 2 cut(s) 123, 819
AfaI GTAC 4 cut(s) 468, 530, 749, 852
AfiI CCNNNNNNNGG 3 cut(s) 278, 638, 841
AgsI TTSAA 6 cut(s) 15, 82, 109, 278, 290, 646
AjnI CCWGG 5 cut(s) 154, 294, 363, 822, 909
AjuI GAANNNNNNNTTGG 1 cut(s) 923
Alw21I GWGCWC 3 cut(s) 70, 385, 886
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 3 cut(s) 663, 839, 852
Aor13HI TCCGGA 1 cut(s) 692
AoxI GGCC 2 cut(s) 397, 734
ApaI GGGCCC 1 cut(s) 738
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 72, 259, 582, 808
ApoI RAATTY 2 cut(s) 416, 485
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 2 cut(s) 734, 735
AsuHPI GGTGA 2 cut(s) 311, 782
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 2 cut(s) 70, 738
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 2 cut(s) 738, 886
BarI GAAGNNNNNNTAC 2 cut(s) 384, 416
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvI GCAGC 4 cut(s) 84, 271, 594, 795
BccI CCATC 5 cut(s) 54, 218, 371, 563, 632
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 5 cut(s) 156, 296, 365, 824, 911
BfaI CTAG 4 cut(s) 440, 671, 707, 887
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 6 cut(s) 73, 150, 260, 583, 586, 809
BlsI GCNGC 6 cut(s) 74, 151, 261, 584, 587, 810
Bme1390I CCNGG 5 cut(s) 156, 296, 365, 824, 911
BmgT120I GGNCC 2 cut(s) 734, 735
BmiI GGNNCC 3 cut(s) 270, 736, 846
BmrFI CCNGG 5 cut(s) 156, 296, 365, 824, 911
BmsI GCATC 2 cut(s) 670, 924
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 4 cut(s) 74, 184, 369, 577
BpuEI CTTGAG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 2 cut(s) 123, 819
BsaJI CCNNGG 6 cut(s) 154, 295, 505, 822, 823, 910
BsaWI WCCGGW 3 cut(s) 39, 692, 834
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 638, 841
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 692
BseBI CCWGG 5 cut(s) 156, 296, 365, 824, 911
BseDI CCNNGG 6 cut(s) 154, 295, 505, 822, 823, 910
BseGI GGATG 2 cut(s) 65, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 278, 638, 841
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 2 cut(s) 77, 116
BseSI GKGCMC 2 cut(s) 70, 738
BseXI GCAGC 4 cut(s) 84, 271, 594, 795
BseYI CCCAGC 2 cut(s) 196, 429
Bsh1285I CGRYCG 1 cut(s) 796
BshFI GGCC 2 cut(s) 399, 736
BsiEI CGRYCG 1 cut(s) 796
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BsiSI CCGG 3 cut(s) 40, 693, 835
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 3 cut(s) 278, 638, 841
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 2 cut(s) 399, 736
Bsp120I GGGCCC 1 cut(s) 734
Bsp1286I GDGCHC 4 cut(s) 70, 385, 738, 886
Bsp13I TCCGGA 1 cut(s) 692
Bsp1407I TGTACA 2 cut(s) 747, 850
Bsp143I GATC 5 cut(s) 93, 139, 655, 703, 844
BspACI CCGC 9 cut(s) 25, 130, 150, 236, 321, 443, 586, 796, 806
BspANI GGCC 2 cut(s) 399, 736
BspEI TCCGGA 1 cut(s) 692
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 3 cut(s) 270, 736, 846
BspPI GGATC 3 cut(s) 663, 839, 852
BsrBI CCGCTC 1 cut(s) 798
BsrGI TGTACA 2 cut(s) 747, 850
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 6 cut(s) 154, 295, 505, 822, 823, 910
BssMI GATC 5 cut(s) 93, 139, 655, 703, 844
BssNI GRCGYC 2 cut(s) 123, 819
Bst2UI CCWGG 5 cut(s) 156, 296, 365, 824, 911
Bst4CI ACNGT 2 cut(s) 350, 533
BstACI GRCGYC 2 cut(s) 123, 819
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 2 cut(s) 747, 850
BstC8I GCNNGC 3 cut(s) 66, 70, 668
BstDEI CTNAG 1 cut(s) 651
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 2 cut(s) 65, 418
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 5 cut(s) 96, 142, 658, 706, 847
BstMBI GATC 5 cut(s) 93, 139, 655, 703, 844
BstMCI CGRYCG 1 cut(s) 796
BstMWI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 667
BstNI CCWGG 5 cut(s) 156, 296, 365, 824, 911
BstNSI RCATGY 1 cut(s) 768
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 5 cut(s) 154, 294, 363, 822, 909
BstSLI GKGCMC 2 cut(s) 70, 738
BstV1I GCAGC 4 cut(s) 84, 271, 594, 795
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 2 cut(s) 399, 736
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 2 cut(s) 243, 647
BtsCI GGATG 2 cut(s) 65, 418
Cac8I GCNNGC 3 cut(s) 66, 70, 668
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 734, 735
CseI GACGC 2 cut(s) 112, 827
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 4 cut(s) 467, 529, 748, 851
CviAII CATG 4 cut(s) 404, 409, 464, 765
CviQI GTAC 4 cut(s) 467, 529, 748, 851
DdeI CTNAG 1 cut(s) 651
DpnI GATC 5 cut(s) 95, 141, 657, 705, 846
DpnII GATC 5 cut(s) 93, 139, 655, 703, 844
Ecl136II GAGCTC 1 cut(s) 884
Eco24I GRGCYC 2 cut(s) 738, 886
Eco32I GATATC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 345
Eco81I CCTNAGG 1 cut(s) 651
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 734
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 5 cut(s) 154, 294, 363, 822, 909
EcoRV GATATC 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 738, 886
FaeI CATG 4 cut(s) 407, 412, 467, 768
FaqI GGGAC 1 cut(s) 412
FatI CATG 4 cut(s) 403, 408, 463, 764
FauI CCCGC 3 cut(s) 18, 123, 436
Fnu4HI GCNGC 6 cut(s) 73, 150, 260, 583, 586, 809
FokI GGATG 2 cut(s) 72, 425
FriOI GRGCYC 2 cut(s) 738, 886
Fsp4HI GCNGC 6 cut(s) 73, 150, 260, 583, 586, 809
FspBI CTAG 4 cut(s) 440, 671, 707, 887
GlaI GCGC 1 cut(s) 76
GluI GCNGC 6 cut(s) 73, 150, 260, 583, 586, 809
GsaI CCCAGC 2 cut(s) 200, 433
GsuI CTGGAG 4 cut(s) 74, 184, 369, 577
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 399, 736
HapII CCGG 3 cut(s) 40, 693, 835
HgaI GACGC 2 cut(s) 112, 827
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 2 cut(s) 123, 819
Hin1II CATG 4 cut(s) 407, 412, 467, 768
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HindIII AAGCTT 2 cut(s) 9, 900
HinfI GANTC 5 cut(s) 166, 215, 460, 788, 944
HpaII CCGG 3 cut(s) 40, 693, 835
HphI GGTGA 2 cut(s) 311, 782
Hpy166II GTNNAC 2 cut(s) 68, 748
Hpy188I TCNGA 4 cut(s) 214, 273, 338, 793
Hpy188III TCNNGA 9 cut(s) 91, 386, 404, 559, 635, 693, 707, 887, 948
Hpy8I GTNNAC 2 cut(s) 68, 748
Hpy99I CGWCG 4 cut(s) 101, 122, 140, 350
HpyAV CCTTC 5 cut(s) 50, 88, 272, 410, 725
HpyCH4III ACNGT 2 cut(s) 350, 533
HpyCH4V TGCA 7 cut(s) 68, 259, 354, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 667
HpyF3I CTNAG 1 cut(s) 651
Hsp92I GRCGYC 2 cut(s) 123, 819
Hsp92II CATG 4 cut(s) 407, 412, 467, 768
HspAI GCGC 1 cut(s) 75
Kpn2I TCCGGA 1 cut(s) 692
Kzo9I GATC 5 cut(s) 93, 139, 655, 703, 844
LmnI GCTCC 6 cut(s) 90, 274, 388, 695, 803, 837
Lsp1109I GCAGC 4 cut(s) 84, 271, 594, 795
LweI GCATC 2 cut(s) 670, 924
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 4 cut(s) 440, 671, 707, 887
MaeIII GTNAC 5 cut(s) 391, 457, 559, 770, 830
MalI GATC 5 cut(s) 95, 141, 657, 705, 846
MbiI CCGCTC 1 cut(s) 798
MboI GATC 5 cut(s) 93, 139, 655, 703, 844
MboII GAAGA 3 cut(s) 65, 515, 907
MfeI CAATTG 2 cut(s) 453, 861
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 4 cut(s) 70, 385, 738, 886
MluCI AATT 6 cut(s) 185, 416, 453, 485, 622, 861
MlyI GAGTC 3 cut(s) 175, 454, 782
MmeI TCCRAC 3 cut(s) 708, 816, 921
MnlI CCTC 8 cut(s) 94, 98, 469, 595, 642, 700, 785, 879
MroI TCCGGA 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 2 cut(s) 449, 471
MspA1I CMGCKG 3 cut(s) 323, 538, 808
MspI CCGG 3 cut(s) 40, 693, 835
MspR9I CCNGG 5 cut(s) 156, 296, 365, 824, 911
MunI CAATTG 2 cut(s) 453, 861
MvaI CCWGG 5 cut(s) 156, 296, 365, 824, 911
MwoI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 667
NdeII GATC 5 cut(s) 93, 139, 655, 703, 844
NlaIII CATG 4 cut(s) 407, 412, 467, 768
NlaIV GGNNCC 3 cut(s) 270, 736, 846
NmuCI GTSAC 3 cut(s) 457, 559, 770
NspI RCATGY 1 cut(s) 768
PagI TCATGA 1 cut(s) 403
PasI CCCWGGG 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 215, 944
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 6 cut(s) 74, 151, 261, 584, 587, 810
PleI GAGTC 3 cut(s) 174, 454, 782
PpsI GAGTC 3 cut(s) 174, 454, 782
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 5 cut(s) 154, 294, 363, 822, 909
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 196, 429
PspGI CCWGG 5 cut(s) 154, 294, 363, 822, 909
PspN4I GGNNCC 3 cut(s) 270, 736, 846
PspOMI GGGCCC 1 cut(s) 734
PspPI GGNCC 2 cut(s) 734, 735
PsuI RGATCY 2 cut(s) 655, 844
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 4 cut(s) 468, 530, 749, 852
RsaNI GTAC 4 cut(s) 467, 529, 748, 851
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 449, 471
SatI GCNGC 6 cut(s) 73, 150, 260, 583, 586, 809
Sau3AI GATC 5 cut(s) 93, 139, 655, 703, 844
Sau96I GGNCC 2 cut(s) 734, 735
SchI GAGTC 3 cut(s) 175, 454, 782
ScrFI CCNGG 5 cut(s) 156, 296, 365, 824, 911
SduI GDGCHC 4 cut(s) 70, 385, 738, 886
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 2 cut(s) 670, 924
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 6 cut(s) 185, 416, 453, 485, 622, 861
SsiI CCGC 9 cut(s) 25, 130, 150, 236, 321, 443, 586, 796, 806
SspI AATATT 1 cut(s) 112
SspMI CTAG 4 cut(s) 440, 671, 707, 887
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 5 cut(s) 154, 294, 363, 822, 909
TaaI ACNGT 2 cut(s) 350, 533
TaqI TCGA 4 cut(s) 96, 117, 138, 345
TasI AATT 6 cut(s) 185, 416, 453, 485, 622, 861
TatI WGTACW 4 cut(s) 466, 528, 747, 850
TauI GCSGC 2 cut(s) 152, 588
TfiI GAWTC 2 cut(s) 215, 944
Tru1I TTAA 2 cut(s) 449, 471
Tru9I TTAA 2 cut(s) 449, 471
TseFI GTSAC 3 cut(s) 457, 559, 770
TseI GCWGC 4 cut(s) 72, 259, 582, 808
Tsp45I GTSAC 3 cut(s) 457, 559, 770
TspDTI ATGAA 4 cut(s) 198, 392, 429, 908
TspGWI ACGGA 1 cut(s) 494
Tth111I GACNNNGTC 1 cut(s) 122
VneI GTGCAC 1 cut(s) 66
XapI RAATTY 2 cut(s) 416, 485
XbaI TCTAGA 2 cut(s) 706, 886
XceI RCATGY 1 cut(s) 768
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 4 cut(s) 440, 671, 707, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.