pycom04g13400

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0020862
Physical Location & Seq
Reverse (-)
16364540 .. 16366799
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13400.3

Sequence Viewer

Length: 951 bp
ATGGGTAAAGCTTCAAGGGATAAGAGGGATATCTACTACCGGAAAGCAAAGGAAGAAGGATGGCGTGCACGCAGCGCCTTCAAGCTCCTCCAGATCGACGAGGAGTTCAATATTTTCGACGGCGTCAAGCGCGTCGTCGATCTCTGTGCCGCCCCTGGTAGCTGGAGTCAGGTTTTGAGCAGGAAATTGTATCTCCCAGCAAAGAGTTCATCTGATTCGAAGGATGGCGATGTGCCTCTTATCGTAGCTATTGATTTGCAGCCCATGGCTCCAATTGAAGGTGTGATTCAAGTGCAGGGTGATATAACTAATGCCCGAACTGCCGAAGTGGTCATCAGACATTTCGACGGCTGCAAAGCTGACCTGGTTGTGTGTGATGGTGCTCCAGACGTTACTGGCCTTCATGACATGGATGAATTTGTTCAGTCCCAGTTGATACTAGCAGGTTTAACCATTGTGACTCATGTACTGAAAGAAGGTGGTAAGTTTATAGCAAAGATATTCCGTGGGAAAGATACAAGTCTTCTGTACTGTCAGCTGAAATTATTTTTCCCTGTTGTGACTTTTGCAAAGCCGAAAAGCAGCCGCAACTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTACTCCCCTCCTGATGGGTTCAACCCTAAGGATCTGCATCGCCTGCTAGAAAAAGTGGGAAGCCCCTCCGGAGCAGATGATCTAGATTGCAGTAGCGGGTGGTTGGAAGGGCCAAATAAGGTGTACATTCCATTTTTAGCATGCGGTGACCTCAGTGGGTATGACTCTGACCGCTCCTATCCGCTGCCTAAAGACGCCCAGGGAACTTACCAGAGTTTGGATCCCGTACAGCCCCCAATCGCCCCTCCCTATAAGAGAGCTCTGGAAATGAAGAAAGCTTCCAGCCAGGGCATTACAGAGCTTGAGAAGCTTTCGTTGGAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.72

Weight (kDa)

5.7

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020862)

Species Orthologous Gene IDs
malus_domestica MD04G1148100.v1.1 MD12G1161600.v1.1
prunus_persica Prupe.6G272000_v2.0.a1
pyrus_communis pycom04g13400 pycom12g15340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 434
AccB7I CCANNNNNTGG 1 cut(s) 841
AccBSI CCGCTC 1 cut(s) 798
AccII CGCG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 692
AciI CCGC 6 cut(s) 150, 586, 720, 768, 796, 806
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 1 cut(s) 416
AcyI GRCGYC 2 cut(s) 123, 819
AfaI GTAC 4 cut(s) 468, 530, 749, 852
AfiI CCNNNNNNNGG 3 cut(s) 278, 638, 841
AgsI TTSAA 6 cut(s) 15, 82, 109, 278, 290, 646
AjnI CCWGG 4 cut(s) 154, 363, 822, 909
AjuI GAANNNNNNNTTGG 1 cut(s) 923
Alw21I GWGCWC 3 cut(s) 70, 385, 886
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 3 cut(s) 663, 839, 852
Aor13HI TCCGGA 1 cut(s) 692
AoxI GGCC 2 cut(s) 397, 734
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 5 cut(s) 72, 259, 351, 582, 808
ApoI RAATTY 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 77, 132
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 2 cut(s) 311, 782
AsuII TTCGAA 1 cut(s) 218
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 70
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvI GCAGC 5 cut(s) 84, 271, 338, 594, 795
BccI CCATC 4 cut(s) 54, 218, 371, 632
BceAI ACGGC 2 cut(s) 136, 364
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 4 cut(s) 156, 365, 824, 911
BfaI CTAG 3 cut(s) 440, 671, 707
BfoI RGCGCY 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 7 cut(s) 73, 150, 260, 352, 583, 586, 809
BlsI GCNGC 7 cut(s) 74, 151, 261, 353, 584, 587, 810
Bme1390I CCNGG 4 cut(s) 156, 365, 824, 911
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 4 cut(s) 156, 365, 824, 911
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 515
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 4 cut(s) 74, 184, 369, 577
Bpu14I TTCGAA 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 2 cut(s) 123, 819
BsaJI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BsaWI WCCGGW 2 cut(s) 39, 692
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 638, 841
Bse1I ACTGG 2 cut(s) 400, 430
Bse21I CCTNAGG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 692
BseBI CCWGG 4 cut(s) 156, 365, 824, 911
BseDI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BseGI GGATG 3 cut(s) 65, 229, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 278, 638, 841
BseMII CTCAG 1 cut(s) 790
BseNI ACTGG 2 cut(s) 400, 430
BseRI GAGGAG 2 cut(s) 77, 116
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 5 cut(s) 84, 271, 338, 594, 795
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 399, 736
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BsiSI CCGG 2 cut(s) 40, 693
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 3 cut(s) 278, 638, 841
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 2 cut(s) 399, 736
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 3 cut(s) 70, 385, 886
Bsp13I TCCGGA 1 cut(s) 692
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 5 cut(s) 93, 139, 655, 703, 844
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 6 cut(s) 150, 586, 720, 768, 796, 806
BspANI GGCC 2 cut(s) 399, 736
BspCNI CTCAG 1 cut(s) 789
BspEI TCCGGA 1 cut(s) 692
BspFNI CGCG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 663, 839, 852
BspT104I TTCGAA 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 798
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 2 cut(s) 400, 430
BssECI CCNNGG 6 cut(s) 154, 264, 505, 822, 823, 910
BssMI GATC 5 cut(s) 93, 139, 655, 703, 844
BssNI GRCGYC 2 cut(s) 123, 819
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 4 cut(s) 156, 365, 824, 911
Bst4CI ACNGT 1 cut(s) 533
BstACI GRCGYC 2 cut(s) 123, 819
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 747
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 66, 70, 668, 766
BstDEI CTNAG 2 cut(s) 651, 776
BstDSI CCRYGG 2 cut(s) 264, 505
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 3 cut(s) 65, 229, 418
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 132
BstKTI GATC 5 cut(s) 96, 142, 658, 706, 847
BstMBI GATC 5 cut(s) 93, 139, 655, 703, 844
BstMWI GCNNNNNNNGC 6 cut(s) 74, 129, 320, 594, 667, 931
BstNI CCWGG 4 cut(s) 156, 365, 824, 911
BstNSI RCATGY 1 cut(s) 768
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 4 cut(s) 154, 363, 822, 909
BstSLI GKGCMC 1 cut(s) 70
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 5 cut(s) 84, 271, 338, 594, 795
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 2 cut(s) 399, 736
BtgI CCRYGG 2 cut(s) 264, 505
BtgZI GCGATG 2 cut(s) 243, 647
BtsCI GGATG 3 cut(s) 65, 229, 418
BtsIMutI CAGTG 1 cut(s) 784
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 66, 70, 668, 766
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 77, 132
Cfr13I GGNCC 1 cut(s) 734
CseI GACGC 3 cut(s) 112, 121, 827
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 4 cut(s) 467, 529, 748, 851
CviAII CATG 5 cut(s) 265, 404, 409, 464, 765
CviQI GTAC 4 cut(s) 467, 529, 748, 851
DdeI CTNAG 2 cut(s) 651, 776
DpnI GATC 5 cut(s) 95, 141, 657, 705, 846
DpnII GATC 5 cut(s) 93, 139, 655, 703, 844
Ecl136II GAGCTC 1 cut(s) 884
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 886
Eco32I GATATC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 884
Eco81I CCTNAGG 1 cut(s) 651
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 884
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 4 cut(s) 154, 363, 822, 909
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 5 cut(s) 268, 407, 412, 467, 768
FaqI GGGAC 1 cut(s) 412
FatI CATG 5 cut(s) 264, 403, 408, 463, 764
FauI CCCGC 1 cut(s) 713
Fnu4HI GCNGC 7 cut(s) 73, 150, 260, 352, 583, 586, 809
FokI GGATG 3 cut(s) 72, 236, 425
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 7 cut(s) 73, 150, 260, 352, 583, 586, 809
FspBI CTAG 3 cut(s) 440, 671, 707
GlaI GCGC 2 cut(s) 76, 131
GluI GCNGC 7 cut(s) 73, 150, 260, 352, 583, 586, 809
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 4 cut(s) 74, 184, 369, 577
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 399, 736
HapII CCGG 2 cut(s) 40, 693
HgaI GACGC 3 cut(s) 112, 121, 827
HhaI GCGC 2 cut(s) 77, 132
Hin1I GRCGYC 2 cut(s) 123, 819
Hin1II CATG 5 cut(s) 268, 407, 412, 467, 768
Hin6I GCGC 2 cut(s) 75, 130
HinP1I GCGC 2 cut(s) 75, 130
HindIII AAGCTT 3 cut(s) 9, 900, 932
HinfI GANTC 6 cut(s) 166, 215, 286, 460, 788, 944
HpaII CCGG 2 cut(s) 40, 693
HphI GGTGA 2 cut(s) 311, 782
Hpy166II GTNNAC 2 cut(s) 68, 748
Hpy188I TCNGA 3 cut(s) 214, 338, 793
Hpy188III TCNNGA 8 cut(s) 91, 386, 404, 635, 693, 707, 887, 948
Hpy8I GTNNAC 2 cut(s) 68, 748
Hpy99I CGWCG 5 cut(s) 101, 122, 137, 140, 350
HpyAV CCTTC 7 cut(s) 50, 88, 214, 272, 410, 470, 725
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 8 cut(s) 68, 259, 295, 354, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 129, 320, 594, 667, 931
HpyF3I CTNAG 2 cut(s) 651, 776
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 2 cut(s) 123, 819
Hsp92II CATG 5 cut(s) 268, 407, 412, 467, 768
HspAI GCGC 2 cut(s) 75, 130
Kpn2I TCCGGA 1 cut(s) 692
Kzo9I GATC 5 cut(s) 93, 139, 655, 703, 844
LmnI GCTCC 5 cut(s) 90, 274, 388, 695, 803
Lsp1109I GCAGC 5 cut(s) 84, 271, 338, 594, 795
LweI GCATC 1 cut(s) 670
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 3 cut(s) 440, 671, 707
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 391, 457, 559, 770
MalI GATC 5 cut(s) 95, 141, 657, 705, 846
MbiI CCGCTC 1 cut(s) 798
MboI GATC 5 cut(s) 93, 139, 655, 703, 844
MboII GAAGA 3 cut(s) 65, 515, 907
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 3 cut(s) 70, 385, 886
MluCI AATT 5 cut(s) 185, 273, 416, 542, 622
MlyI GAGTC 3 cut(s) 175, 454, 782
MmeI TCCRAC 2 cut(s) 708, 921
MnlI CCTC 9 cut(s) 18, 94, 98, 246, 595, 642, 700, 785, 879
MroI TCCGGA 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 538, 808
MspI CCGG 2 cut(s) 40, 693
MspR9I CCNGG 4 cut(s) 156, 365, 824, 911
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 4 cut(s) 156, 365, 824, 911
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 6 cut(s) 74, 129, 320, 594, 667, 931
NcoI CCATGG 1 cut(s) 264
NdeII GATC 5 cut(s) 93, 139, 655, 703, 844
NlaIII CATG 5 cut(s) 268, 407, 412, 467, 768
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 3 cut(s) 457, 559, 770
NspI RCATGY 1 cut(s) 768
NspV TTCGAA 1 cut(s) 218
PaeI GCATGC 1 cut(s) 768
PagI TCATGA 1 cut(s) 403
PasI CCCWGGG 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 3 cut(s) 215, 286, 944
PflFI GACNNNGTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 841
PkrI GCNGC 7 cut(s) 74, 151, 261, 353, 584, 587, 810
PleI GAGTC 3 cut(s) 174, 454, 782
PpsI GAGTC 3 cut(s) 174, 454, 782
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 4 cut(s) 154, 363, 822, 909
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 4 cut(s) 154, 363, 822, 909
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 2 cut(s) 655, 844
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 4 cut(s) 468, 530, 749, 852
RsaNI GTAC 4 cut(s) 467, 529, 748, 851
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 7 cut(s) 73, 150, 260, 352, 583, 586, 809
Sau3AI GATC 5 cut(s) 93, 139, 655, 703, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 3 cut(s) 175, 454, 782
ScrFI CCNGG 4 cut(s) 156, 365, 824, 911
SduI GDGCHC 3 cut(s) 70, 385, 886
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 1 cut(s) 670
SfuI TTCGAA 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SphI GCATGC 1 cut(s) 768
Sse9I AATT 5 cut(s) 185, 273, 416, 542, 622
SsiI CCGC 6 cut(s) 150, 586, 720, 768, 796, 806
SspI AATATT 1 cut(s) 112
SspMI CTAG 3 cut(s) 440, 671, 707
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 154, 363, 822, 909
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 533
TaiI ACGT 1 cut(s) 393
TaqI TCGA 5 cut(s) 96, 117, 138, 218, 345
TasI AATT 5 cut(s) 185, 273, 416, 542, 622
TatI WGTACW 3 cut(s) 466, 528, 747
TauI GCSGC 2 cut(s) 152, 588
TfiI GAWTC 3 cut(s) 215, 286, 944
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 784
TseFI GTSAC 3 cut(s) 457, 559, 770
TseI GCWGC 5 cut(s) 72, 259, 351, 582, 808
Tsp45I GTSAC 3 cut(s) 457, 559, 770
TspDTI ATGAA 4 cut(s) 198, 392, 429, 908
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 784
Tth111I GACNNNGTC 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 841
VneI GTGCAC 1 cut(s) 66
XapI RAATTY 1 cut(s) 416
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 1 cut(s) 768
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 3 cut(s) 440, 671, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.