Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0015891

Physical Location & Seq

Genomic Coordinates Reverse (-)

26364436 .. 26365743

Gene Structure 1308 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1172200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGCCGGAGATCGAGGTCGTCACCGATAACAGAGACGGCTCCAGCGCCGGCGAGGTCAACCTATTCGGTAAGTACGAGGTCGGGAGGCTTCTGGGCTGCGGGGCTTTCGCGAAGGTGTACCACGCTCGGAATGTCCACACGGGGCAGAGCGTGGCGATCAAGGCCGTCAGCAAGCAGAAGGTCGTGAAGGACGGGTTCACGGCTCACGTTAAGCGGGAGATCTCCATCATGCGCCGGCTGCGCCACCCGCATATCGTGAAGCTGTTCGAGGTTTTAGCGACGAAGACCAAGATCTATTTCATCATGGAGTTTGCAAAAGGCGGAGAGCTCTTTGCAAAGATCTCGAAGGGCCGGTTCAGCGAGGATCTCAGCCGTCGGTACTTCCAGCAGCTGATCTCCGCCGTCGGATACTGCCACTCGCGGGGAGTGTTCCACCGTGATTTGAAACCTGAGAATCTGCTCCTCGACGAGAACTGGAATTTGAAAGTCTCGGACTTCGGCCTCAGCGCCGTGACGGAGCAGGTCCGGCCCGACGGTCTCCTCTACACGCTTTGCGGGACTCCTGCGTACGTGGCGCCGGAGATTCTAGCGAAGAAGGGGTACGACGGCGCGAAGATCGACATCTGGTCGTGCGGCATCGTTTTGTTCGTGCTTAACGCCGGTTACCTGCCGTTTAACGACCCGAATCTCATGGTGATGTACCGGAAAATTTACAAGGGAGATTATCGGGTCCCGAAATGGACGTCGCCCGATCTGAAGCGCCTGATATCTCGGCTTCTCGACACGAACTCCGAGACGCGGATAACCGTCGACGAGATTTTGAAGGACCCGTGGTTCGCGAAGGGTTACCAGGAGATTAAATTCCACTTCGAGGACTTCGACTTGAAGGACATGCGGGACGGGGACAGCGACGAGACGTCGTCGTTGAACGCGTTTGATTTGATTTCGTTCTCGTCGGGGTTCGATATATCGGGTTTGTTTAGCAAGTCCGGGATATCGGACTGCGGGGAGCGGTTCGTGTCGGCGGAGACGCCGGAGAGGATAATGGAGAAGGTGGAGGAGGTGGCTAAGGCGGGGGACATGGCGGTGACGGAGAAGAAGAGCTGGGGGGCGAAACTGGCGGGGCAGAATGGGAATTTTGTTGTAATGATTGGGATTTACCGGTTAACGGAGAAGCTGGTTGTGGTGGAGTTGAAATTAAGGCAAAGGGAAGCTGAATGTGGGCCGCAAATATGGAAAGACAAGCTGAGGCCGGAGCTGAGCTGTTTGATGTACCGACCGGAAGAGCCAGTCGCCGGTGACTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0030054 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04113 ko04138 map04113 map04138

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

436

Amino Acids

49.15

Weight (kDa)

7.98

Isoelectric Point (pI)

31.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	25 - 279	1.5e-73	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 275	4.1e-36	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	121 - 267	6.1e-07	Kinase-like
NAF	PF03822	307 - 364	1.2e-20	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01820
fragaria_vesca	FvH4_6g10160
malus_domestica	MD04G1172200.v1.1 MD12G1185400.v1.1
prunus_persica	Prupe.6G290700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0460881
rosa_laevigata	RLG00000024971
rosa_multiflora	Rmu_co8354755.1_g000001 Rmu_sc0008258.1_g000006
rosa_roxburghii	Rroxscaffold_6G00419060
rosa_rugosa	Rorug03G0047000
rosa_samantha	Rh3AG104200 Rh3BG107900 Rh3DG109700
rosa_wichuraiana	Rw3G008870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	746, 920
Acc36I	ACCTGC	2 cut(s)	511, 675
AccB1I	GGYRCC	1 cut(s)	574
AccBSI	CCGCTC	1 cut(s)	1012
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
AclWI	GGATC	1 cut(s)	372
AcsI	RAATTY	4 cut(s)	478, 708, 860, 1135
AcuI	CTGAAG	1 cut(s)	776
AcyI	GRCGYC	4 cut(s)	575, 743, 917, 1031
AfaI	GTAC	7 cut(s)	74, 119, 380, 569, 602, 701, 1274
AfiI	CCNNNNNNNGG	5 cut(s)	421, 798, 871, 1168, 1295
AflIII	ACRYGT	1 cut(s)	930
AgeI	ACCGGT	1 cut(s)	1161
AgsI	TTSAA	6 cut(s)	445, 484, 823, 886, 928, 1195
AhdI	GACNNNNNGTC	2 cut(s)	625, 916
AjnI	CCWGG	1 cut(s)	849
AjuI	GAANNNNNNNTTGG	2 cut(s)	281, 313
AloI	GAACNNNNNNTCC	2 cut(s)	818, 850
AluBI	AGCT	9 cut(s)	262, 328, 391, 1104, 1177, 1214, 1246, 1258, 1263
AluI	AGCT	9 cut(s)	262, 328, 391, 1104, 1177, 1214, 1246, 1258, 1263
Alw21I	GWGCWC	1 cut(s)	330
Alw26I	GTCTC	6 cut(s)	27, 493, 542, 788, 908, 1022
AlwI	GGATC	1 cut(s)	372
AlwNI	CAGNNNCTG	1 cut(s)	391
AoxI	GGCC	6 cut(s)	162, 349, 499, 527, 1223, 1250
ApeKI	GCWGC	3 cut(s)	96, 238, 388
ApoI	RAATTY	4 cut(s)	478, 708, 860, 1135
ArsI	GACNNNNNNTTYG	4 cut(s)	728, 760, 818, 850
AsiGI	ACCGGT	1 cut(s)	1161
Asp700I	GAANNNNTTC	1 cut(s)	263
AspLEI	GCGC	7 cut(s)	47, 234, 243, 509, 577, 611, 762
AspS9I	GGNCC	6 cut(s)	349, 523, 528, 730, 826, 1223
AsuC2I	CCSGG	1 cut(s)	991
AsuHPI	GGTGA	3 cut(s)	13, 706, 1099
AvaII	GGWCC	3 cut(s)	523, 730, 826
BanI	GGYRCC	1 cut(s)	574
BanII	GRGCYC	1 cut(s)	330
BarI	GAAGNNNNNNTAC	2 cut(s)	584, 616
BbsI	GAAGAC	1 cut(s)	290
Bbv12I	GWGCWC	1 cut(s)	330
BbvCI	CCTCAGC	2 cut(s)	503, 1247
BbvI	GCAGC	3 cut(s)	83, 225, 400
BccI	CCATC	1 cut(s)	233
BceAI	ACGGC	8 cut(s)	52, 149, 216, 357, 386, 494, 622, 655
BcgI	CGANNNNNNTGC	2 cut(s)	324, 358
BciT130I	CCWGG	1 cut(s)	851
BciVI	GTATCC	1 cut(s)	401
BcnI	CCSGG	1 cut(s)	991
BcoDI	GTCTC	6 cut(s)	27, 493, 542, 788, 908, 1022
BfaI	CTAG	1 cut(s)	587
BfoI	RGCGCY	4 cut(s)	48, 510, 578, 763
BfuAI	ACCTGC	2 cut(s)	511, 675
BfuI	GTATCC	1 cut(s)	401
BglII	AGATCT	3 cut(s)	219, 291, 339
BisI	GCNGC	5 cut(s)	97, 239, 389, 634, 1226
BlpI	GCTNAGC	1 cut(s)	1259
BlsI	GCNGC	5 cut(s)	98, 240, 390, 635, 1227
Bme1390I	CCNGG	2 cut(s)	851, 991
Bme18I	GGWCC	3 cut(s)	523, 730, 826
BmeRI	GACNNNNNGTC	2 cut(s)	625, 916
BmgT120I	GGNCC	6 cut(s)	349, 523, 528, 730, 826, 1223
BmiI	GGNNCC	5 cut(s)	40, 576, 731, 732, 828
BmrFI	CCNGG	2 cut(s)	851, 991
BmsI	GCATC	1 cut(s)	645
BpiI	GAAGAC	1 cut(s)	290
BpmI	CTGGAG	1 cut(s)	25
Bpu10I	CCTNAGC	3 cut(s)	503, 1068, 1247
Bpu1102I	GCTNAGC	1 cut(s)	1259
BpuMI	CCSGG	1 cut(s)	991
BsaAI	YACGTR	1 cut(s)	571
BsaBI	GATNNNNATC	2 cut(s)	224, 620
BsaHI	GRCGYC	4 cut(s)	575, 743, 917, 1031
BsaI	GGTCTC	1 cut(s)	542
BsaJI	CCNNGG	1 cut(s)	830
BsaWI	WCCGGW	3 cut(s)	702, 1161, 1279
BsaXI	ACNNNNNCTCC	2 cut(s)	773, 803
Bsc4I	CCNNNNNNNGG	5 cut(s)	421, 798, 871, 1168, 1295
Bse118I	RCCGGY	6 cut(s)	47, 234, 351, 659, 1161, 1295
Bse1I	ACTGG	3 cut(s)	479, 1122, 1289
Bse8I	GATNNNNATC	2 cut(s)	224, 620
BseBI	CCWGG	1 cut(s)	851
BseDI	CCNNGG	1 cut(s)	830
BseJI	GATNNNNATC	2 cut(s)	224, 620
BseLI	CCNNNNNNNGG	5 cut(s)	421, 798, 871, 1168, 1295
BseMII	CTCAG	5 cut(s)	382, 441, 517, 1238, 1250
BseNI	ACTGG	3 cut(s)	479, 1122, 1289
BseRI	GAGGAG	3 cut(s)	452, 530, 1073
BseXI	GCAGC	3 cut(s)	83, 225, 400
BseYI	CCCAGC	1 cut(s)	1104
Bsh1236I	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
Bsh1285I	CGRYCG	1 cut(s)	1280
BshFI	GGCC	6 cut(s)	164, 351, 501, 529, 1225, 1252
BshNI	GGYRCC	1 cut(s)	574
BshTI	ACCGGT	1 cut(s)	1161
BsiEI	CGRYCG	1 cut(s)	1280
BsiHKAI	GWGCWC	1 cut(s)	330
BsiWI	CGTACG	1 cut(s)	567
BslFI	GGGAC	5 cut(s)	571, 716, 911, 917, 1091
BslI	CCNNNNNNNGG	5 cut(s)	421, 798, 871, 1168, 1295
BsmAI	GTCTC	6 cut(s)	27, 493, 542, 788, 908, 1022
BsmBI	CGTCTC	4 cut(s)	27, 788, 908, 1022
BsmFI	GGGAC	5 cut(s)	571, 716, 911, 917, 1091
BsnI	GGCC	6 cut(s)	164, 351, 501, 529, 1225, 1252
Bso31I	GGTCTC	1 cut(s)	542
Bsp1286I	GDGCHC	1 cut(s)	330
Bsp143I	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
Bsp1720I	GCTNAGC	1 cut(s)	1259
Bsp68I	TCGCGA	2 cut(s)	110, 839
BspANI	GGCC	6 cut(s)	164, 351, 501, 529, 1225, 1252
BspCNI	CTCAG	5 cut(s)	381, 442, 516, 1239, 1251
BspFNI	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
BspLI	GGNNCC	5 cut(s)	40, 576, 731, 732, 828
BspMI	ACCTGC	2 cut(s)	511, 675
BspPI	GGATC	1 cut(s)	372
BspQI	GCTCTTC	2 cut(s)	1094, 1278
BspT107I	GGYRCC	1 cut(s)	574
BspTNI	GGTCTC	1 cut(s)	542
BsrBI	CCGCTC	1 cut(s)	1012
BsrFI	RCCGGY	6 cut(s)	47, 234, 351, 659, 1161, 1295
BsrI	ACTGG	3 cut(s)	479, 1122, 1289
BssAI	RCCGGY	6 cut(s)	47, 234, 351, 659, 1161, 1295
BssECI	CCNNGG	1 cut(s)	830
BssMI	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
BssNI	GRCGYC	4 cut(s)	575, 743, 917, 1031
Bst2UI	CCWGG	1 cut(s)	851
Bst4CI	ACNGT	4 cut(s)	437, 536, 808, 1304
Bst6I	CTCTTC	2 cut(s)	1094, 1278
BstACI	GRCGYC	4 cut(s)	575, 743, 917, 1031
BstBAI	YACGTR	1 cut(s)	571
BstC8I	GCNNGC	3 cut(s)	49, 173, 236
BstDEI	CTNAG	6 cut(s)	368, 450, 503, 1068, 1247, 1259
BstDSI	CCRYGG	1 cut(s)	830
BstEII	GGTNACC	2 cut(s)	662, 845
BstFNI	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
BstH2I	RGCGCY	4 cut(s)	48, 510, 578, 763
BstHHI	GCGC	7 cut(s)	47, 234, 243, 509, 577, 611, 762
BstKTI	GATC	9 cut(s)	12, 159, 222, 294, 342, 367, 396, 618, 754
BstMAI	GTCTC	6 cut(s)	27, 493, 542, 788, 908, 1022
BstMBI	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
BstMCI	CGRYCG	1 cut(s)	1280
BstMWI	GCNNNNNNNGC	8 cut(s)	161, 238, 240, 247, 357, 526, 572, 1118
BstNI	CCWGG	1 cut(s)	851
BstNSI	RCATGY	1 cut(s)	895
BstPI	GGTNACC	2 cut(s)	662, 845
BstSCI	CCNGG	2 cut(s)	849, 989
BstUI	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
BstV1I	GCAGC	3 cut(s)	83, 225, 400
BstV2I	GAAGAC	1 cut(s)	290
BstX2I	RGATCY	4 cut(s)	219, 291, 339, 364
BstYI	RGATCY	4 cut(s)	219, 291, 339, 364
BsuI	GTATCC	1 cut(s)	401
BsuRI	GGCC	6 cut(s)	164, 351, 501, 529, 1225, 1252
BtgI	CCRYGG	1 cut(s)	830
BtuMI	TCGCGA	2 cut(s)	110, 839
BveI	ACCTGC	2 cut(s)	511, 675
Cac8I	GCNNGC	3 cut(s)	49, 173, 236
CaiI	CAGNNNCTG	1 cut(s)	391
CfoI	GCGC	7 cut(s)	47, 234, 243, 509, 577, 611, 762
Cfr10I	RCCGGY	6 cut(s)	47, 234, 351, 659, 1161, 1295
Cfr13I	GGNCC	6 cut(s)	349, 523, 528, 730, 826, 1223
CseI	GACGC	2 cut(s)	805, 1039
Csp6I	GTAC	7 cut(s)	73, 118, 379, 568, 601, 700, 1273
CspAI	ACCGGT	1 cut(s)	1161
CviAII	CATG	5 cut(s)	229, 304, 691, 892, 1081
CviQI	GTAC	7 cut(s)	73, 118, 379, 568, 601, 700, 1273
DdeI	CTNAG	6 cut(s)	368, 450, 503, 1068, 1247, 1259
DinI	GGCGCC	1 cut(s)	576
DpnI	GATC	9 cut(s)	11, 158, 221, 293, 341, 366, 395, 617, 753
DpnII	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
DriI	GACNNNNNGTC	2 cut(s)	625, 916
Eam1104I	CTCTTC	2 cut(s)	1094, 1278
Eam1105I	GACNNNNNGTC	2 cut(s)	625, 916
EarI	CTCTTC	2 cut(s)	1094, 1278
EciI	GGCGGA	3 cut(s)	336, 388, 1040
Ecl136II	GAGCTC	1 cut(s)	328
Eco24I	GRGCYC	1 cut(s)	330
Eco31I	GGTCTC	1 cut(s)	542
Eco32I	GATATC	2 cut(s)	768, 996
Eco47I	GGWCC	3 cut(s)	523, 730, 826
Eco53kI	GAGCTC	1 cut(s)	328
Eco57I	CTGAAG	1 cut(s)	776
Eco91I	GGTNACC	2 cut(s)	662, 845
EcoICRI	GAGCTC	1 cut(s)	328
EcoO109I	RGGNCCY	2 cut(s)	730, 826
EcoO65I	GGTNACC	2 cut(s)	662, 845
EcoRII	CCWGG	1 cut(s)	849
EcoRV	GATATC	2 cut(s)	768, 996
EcoT38I	GRGCYC	1 cut(s)	330
EgeI	GGCGCC	1 cut(s)	576
EheI	GGCGCC	1 cut(s)	576
Esp3I	CGTCTC	4 cut(s)	27, 788, 908, 1022
FaeI	CATG	5 cut(s)	232, 307, 694, 895, 1084
FaiI	YATR	8 cut(s)	230, 252, 305, 692, 893, 968, 1082, 1234
FaqI	GGGAC	5 cut(s)	571, 716, 911, 917, 1091
FatI	CATG	5 cut(s)	228, 303, 690, 891, 1080
FauI	CCCGC	9 cut(s)	92, 207, 255, 414, 548, 888, 998, 1066, 1114
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	5 cut(s)	97, 239, 389, 634, 1226
FriOI	GRGCYC	1 cut(s)	330
Fsp4HI	GCNGC	5 cut(s)	97, 239, 389, 634, 1226
FspBI	CTAG	1 cut(s)	587
GlaI	GCGC	7 cut(s)	46, 233, 242, 508, 576, 610, 761
GluI	GCNGC	5 cut(s)	97, 239, 389, 634, 1226
GsaI	CCCAGC	1 cut(s)	1108
GsuI	CTGGAG	1 cut(s)	25
HaeII	RGCGCY	4 cut(s)	48, 510, 578, 763
HaeIII	GGCC	6 cut(s)	164, 351, 501, 529, 1225, 1252
HgaI	GACGC	2 cut(s)	805, 1039
HhaI	GCGC	7 cut(s)	47, 234, 243, 509, 577, 611, 762
Hin1I	GRCGYC	4 cut(s)	575, 743, 917, 1031
Hin1II	CATG	5 cut(s)	232, 307, 694, 895, 1084
Hin6I	GCGC	7 cut(s)	45, 232, 241, 507, 575, 609, 760
HinP1I	GCGC	7 cut(s)	45, 232, 241, 507, 575, 609, 760
HincII	GTYRAC	3 cut(s)	58, 811, 1167
HindII	GTYRAC	3 cut(s)	58, 811, 1167
HinfI	GANTC	4 cut(s)	454, 559, 583, 685
HpaI	GTTAAC	1 cut(s)	1167
HphI	GGTGA	3 cut(s)	13, 706, 1099
Hpy166II	GTNNAC	6 cut(s)	58, 118, 136, 198, 811, 1167
Hpy188I	TCNGA	6 cut(s)	129, 407, 493, 756, 793, 1000
Hpy188III	TCNNGA	8 cut(s)	82, 109, 184, 256, 343, 733, 779, 838
Hpy8I	GTNNAC	6 cut(s)	58, 118, 136, 198, 811, 1167
HpyAV	CCTTC	9 cut(s)	106, 172, 181, 340, 589, 817, 835, 880, 1045
HpyCH4III	ACNGT	4 cut(s)	437, 536, 808, 1304
HpyCH4IV	ACGT	4 cut(s)	207, 570, 743, 917
HpyCH4V	TGCA	2 cut(s)	314, 335
HpyF10VI	GCNNNNNNNGC	8 cut(s)	161, 238, 240, 247, 357, 526, 572, 1118
HpyF3I	CTNAG	6 cut(s)	368, 450, 503, 1068, 1247, 1259
HpySE526I	ACGT	4 cut(s)	207, 570, 743, 917
Hsp92I	GRCGYC	4 cut(s)	575, 743, 917, 1031
Hsp92II	CATG	5 cut(s)	232, 307, 694, 895, 1084
HspAI	GCGC	7 cut(s)	45, 232, 241, 507, 575, 609, 760
KasI	GGCGCC	1 cut(s)	574
KflI	GGGWCCC	1 cut(s)	730
KroI	GCCGGC	2 cut(s)	47, 234
KroNI	GCCGGC	2 cut(s)	49, 236
KspAI	GTTAAC	1 cut(s)	1167
Kzo9I	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
LguI	GCTCTTC	2 cut(s)	1094, 1278
LmnI	GCTCC	5 cut(s)	44, 465, 517, 1009, 1255
Lsp1109I	GCAGC	3 cut(s)	83, 225, 400
LweI	GCATC	1 cut(s)	645
MaeI	CTAG	1 cut(s)	587
MaeII	ACGT	4 cut(s)	207, 570, 743, 917
MaeIII	GTNAC	6 cut(s)	19, 511, 662, 845, 1087, 1298
MalI	GATC	9 cut(s)	11, 158, 221, 293, 341, 366, 395, 617, 753
MbiI	CCGCTC	1 cut(s)	1012
MboI	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
MboII	GAAGA	6 cut(s)	295, 604, 625, 1108, 1111, 1295
MflI	RGATCY	4 cut(s)	219, 291, 339, 364
MhlI	GDGCHC	1 cut(s)	330
MluCI	AATT	5 cut(s)	478, 708, 860, 1135, 1196
MluI	ACGCGT	1 cut(s)	930
Mly113I	GGCGCC	1 cut(s)	575
MlyI	GAGTC	1 cut(s)	553
MmeI	TCCRAC	1 cut(s)	385
MreI	CGCCGGCG	1 cut(s)	47
MroNI	GCCGGC	2 cut(s)	47, 234
MroXI	GAANNNNTTC	1 cut(s)	263
MseI	TTAA	6 cut(s)	210, 654, 675, 858, 1166, 1199
MslI	CAYNNNNRTG	2 cut(s)	695, 1085
MspA1I	CMGCKG	1 cut(s)	391
MspR9I	CCNGG	2 cut(s)	851, 991
MvaI	CCWGG	1 cut(s)	851
MvnI	CGCG	6 cut(s)	110, 421, 611, 799, 839, 932
MwoI	GCNNNNNNNGC	8 cut(s)	161, 238, 240, 247, 357, 526, 572, 1118
NaeI	GCCGGC	2 cut(s)	49, 236
NarI	GGCGCC	1 cut(s)	575
NciI	CCSGG	1 cut(s)	991
NdeII	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
NgoMIV	GCCGGC	2 cut(s)	47, 234
NlaIII	CATG	5 cut(s)	232, 307, 694, 895, 1084
NlaIV	GGNNCC	5 cut(s)	40, 576, 731, 732, 828
NmeAIII	GCCGAG	1 cut(s)	751
NmuCI	GTSAC	4 cut(s)	19, 511, 1087, 1298
NruI	TCGCGA	2 cut(s)	110, 839
NspI	RCATGY	1 cut(s)	895
PciSI	GCTCTTC	2 cut(s)	1094, 1278
PcsI	WCGNNNNNNNCGW	5 cut(s)	72, 645, 876, 906, 929
PdiI	GCCGGC	2 cut(s)	49, 236
PdmI	GAANNNNTTC	1 cut(s)	263
PfeI	GAWTC	3 cut(s)	454, 583, 685
Pfl23II	CGTACG	1 cut(s)	567
PflFI	GACNNNGTC	1 cut(s)	919
PfoI	TCCNGGA	1 cut(s)	989
PinAI	ACCGGT	1 cut(s)	1161
PkrI	GCNGC	5 cut(s)	98, 240, 390, 635, 1227
PleI	GAGTC	1 cut(s)	553
PluTI	GGCGCC	1 cut(s)	578
PpsI	GAGTC	1 cut(s)	553
Ppu21I	YACGTR	1 cut(s)	571
PpuMI	RGGWCCY	2 cut(s)	730, 826
Psp124BI	GAGCTC	1 cut(s)	330
Psp5II	RGGWCCY	2 cut(s)	730, 826
Psp6I	CCWGG	1 cut(s)	849
PspEI	GGTNACC	2 cut(s)	662, 845
PspFI	CCCAGC	1 cut(s)	1104
PspGI	CCWGG	1 cut(s)	849
PspLI	CGTACG	1 cut(s)	567
PspN4I	GGNNCC	5 cut(s)	40, 576, 731, 732, 828
PspPI	GGNCC	6 cut(s)	349, 523, 528, 730, 826, 1223
PspPPI	RGGWCCY	2 cut(s)	730, 826
PstNI	CAGNNNCTG	1 cut(s)	391
PsuI	RGATCY	4 cut(s)	219, 291, 339, 364
PsyI	GACNNNGTC	1 cut(s)	919
PvuII	CAGCTG	1 cut(s)	391
RruI	TCGCGA	2 cut(s)	110, 839
RsaI	GTAC	7 cut(s)	74, 119, 380, 569, 602, 701, 1274
RsaNI	GTAC	7 cut(s)	73, 118, 379, 568, 601, 700, 1273
RseI	CAYNNNNRTG	2 cut(s)	695, 1085
SacI	GAGCTC	1 cut(s)	330
SalI	GTCGAC	1 cut(s)	809
SapI	GCTCTTC	2 cut(s)	1094, 1278
SaqAI	TTAA	6 cut(s)	210, 654, 675, 858, 1166, 1199
SatI	GCNGC	5 cut(s)	97, 239, 389, 634, 1226
Sau3AI	GATC	9 cut(s)	9, 156, 219, 291, 339, 364, 393, 615, 751
Sau96I	GGNCC	6 cut(s)	349, 523, 528, 730, 826, 1223
SchI	GAGTC	1 cut(s)	553
ScrFI	CCNGG	2 cut(s)	851, 991
SduI	GDGCHC	1 cut(s)	330
SfaNI	GCATC	1 cut(s)	645
SfoI	GGCGCC	1 cut(s)	576
SgrAI	CRCCGGYG	2 cut(s)	47, 1295
SgrDI	CGTCGACG	1 cut(s)	809
SinI	GGWCC	3 cut(s)	523, 730, 826
SmiMI	CAYNNNNRTG	2 cut(s)	695, 1085
Sse9I	AATT	5 cut(s)	478, 708, 860, 1135, 1196
SspDI	GGCGCC	1 cut(s)	574
SspMI	CTAG	1 cut(s)	587
SstI	GAGCTC	1 cut(s)	330
StyD4I	CCNGG	2 cut(s)	849, 989
TaaI	ACNGT	4 cut(s)	437, 536, 808, 1304
TaiI	ACGT	4 cut(s)	210, 573, 746, 920
TasI	AATT	5 cut(s)	478, 708, 860, 1135, 1196
TauI	GCSGC	2 cut(s)	636, 1228
TfiI	GAWTC	3 cut(s)	454, 583, 685
Tru1I	TTAA	6 cut(s)	210, 654, 675, 858, 1166, 1199
Tru9I	TTAA	6 cut(s)	210, 654, 675, 858, 1166, 1199
TseFI	GTSAC	4 cut(s)	19, 511, 1087, 1298
TseI	GCWGC	3 cut(s)	96, 238, 388
Tsp45I	GTSAC	4 cut(s)	19, 511, 1087, 1298
TspDTI	ATGAA	1 cut(s)	289
TspGWI	ACGGA	3 cut(s)	530, 1106, 1184
Tth111I	GACNNNGTC	1 cut(s)	919
VpaK11BI	GGWCC	3 cut(s)	523, 730, 826
XapI	RAATTY	4 cut(s)	478, 708, 860, 1135
XceI	RCATGY	1 cut(s)	895
XmiI	GTMKAC	1 cut(s)	810
XmnI	GAANNNNTTC	1 cut(s)	263
XspI	CTAG	1 cut(s)	587
ZraI	GACGTC	2 cut(s)	744, 918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.