Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015891

Physical Location & Seq

Genomic Coordinates Reverse (-)

26664330 .. 26665845

Gene Structure 1516 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1185400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGCCGGAGATCGACGTCGTCACCGACAATGGCGACGACTCCGGCGCAGACGAGGTTAACCTGTTCGGGAAGTACGAGGTTGGGAGGCTGCTGGGCCGCGGGGCCTTCGCGAAGGTGTACCACGCGCGGAACGTGCACACGGAGCAGAGCGTGGCGATCAAGGCCGTCAGCAAGCAAAAGGTCCTGAAGGGCGGGTTTGCGGCCCACGTTAAGCAGGAGATCTCTATCATGCGCCGTCTTGGGCACCCGCACATCGTGAAGCTCAACGAGGTTTTAGCGACTAAGACCAAGATCTACTTCATCATGGAGTTCGCCAAGGGCGGCGAGCTCTTCACGAAGATCTCCAAGGGGCGGTTCAGCGAGCATCTCAGCCGTCGGTACTTTCAGCAGCTGATCTCCGCCGTCGGATACTGCCACTCGCGGGGAGTGTACCACCGCGATTTGAAGCCCGAGAATCTGCTCCTCGACGAGAACTGGAACTTGAAAGTCTCGGACTTCGGGCTGAGCGCCTTGACGGAGCAGGTCCGGTCAGACGGTCTCCTCCACACACTCTGCGGGACCCCCGGGTACGTGGCGCCGGAGATTCTCGCGAAGAAGGGGTACGACGGCGCGAAGATCGACATCTGGTCGTGCGGCATCATTTTGTTCGTTCTGAACGCGGGCTACCTACCTTTTAACGACCCGAATCTCATGGTGATGTACCGCAAGATTTACAAGGGAGATTATCGGGTTCCGAGATGGACGTCGCCGGAACTGAAGCACCTGATTTCTCGGCTCCTCGACACGAATTCCGAGACGCGGATAACCGTCGACGAGATTTTGAAGGACCCGTGGTTCGAGAAGGGGTACCGGGAGACTAAATTCGACTTCGAGGACTTTGGCTTGGAGGACATGCGGGACGGGGACAGCGACAAAACGTCGTCGTTGAACGCGTTTGATTTGATTTCGTTCTCGTCTGGGTTCGATATCTCGGGGTTGTTTAGCAAGTCCGCGATATCCGACTGCGGGGAGCGGTTCGTGTCGGCGGAGACGCCGGAGAGGATAATGGAGAAGGTGGAGGAGGTGGCGAAGGCGGGGGACGTGTCGGTGACGGAGAAGAAGAGCTGGGGGGCGAAATTGGCGGGGAAGAATGGGAATTTTGTCGTGATGGTTGGGATTTACCGGTTAACGGAGAAGCTGGTCGTGGTGGAGTTGAATTTAAGGCAAAGAGAAGTTGAAAGTGGGCCGCAAATATGGATAGATAAGCTGAGGCCGCAGCTAAACTGTTTGATGTACCGACCGGGAGAGCCGGTCGCCGGTGACTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0030054 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04113 ko04138 map04113 map04138

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

436

Amino Acids

49.0

Weight (kDa)

6.81

Isoelectric Point (pI)

28.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	25 - 279	1.5e-73	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 275	4.3e-36	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	119 - 267	1.1e-06	Kinase-like
NAF	PF03822	307 - 363	3.3e-20	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01820
fragaria_vesca	FvH4_6g10160
malus_domestica	MD04G1172200.v1.1 MD12G1185400.v1.1
prunus_persica	Prupe.6G290700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0460881
rosa_laevigata	RLG00000024971
rosa_multiflora	Rmu_co8354755.1_g000001 Rmu_sc0008258.1_g000006
rosa_roxburghii	Rroxscaffold_6G00419060
rosa_rugosa	Rorug03G0047000
rosa_samantha	Rh3AG104200 Rh3BG107900 Rh3DG109700
rosa_wichuraiana	Rw3G008870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	18, 746
Acc36I	ACCTGC	1 cut(s)	511
Acc65I	GGTACC	1 cut(s)	846
AccB1I	GGYRCC	3 cut(s)	243, 574, 846
AccBSI	CCGCTC	1 cut(s)	1012
AccI	GTMKAC	1 cut(s)	810
AcsI	RAATTY	4 cut(s)	787, 860, 1135, 1195
AcuI	CTGAAG	2 cut(s)	206, 776
AcyI	GRCGYC	4 cut(s)	15, 575, 743, 1031
AdeI	CACNNNGTG	1 cut(s)	256
AfaI	GTAC	9 cut(s)	74, 119, 380, 431, 569, 602, 701, 848, 1274
AfiI	CCNNNNNNNGG	6 cut(s)	351, 421, 798, 1005, 1168, 1295
AflIII	ACRYGT	2 cut(s)	930, 1080
AgeI	ACCGGT	1 cut(s)	1161
AgsI	TTSAA	6 cut(s)	445, 484, 823, 928, 1195, 1217
AhdI	GACNNNNNGTC	2 cut(s)	625, 916
AjiI	CACGTC	1 cut(s)	1081
AjuI	GAANNNNNNNTTGG	4 cut(s)	281, 313, 338, 370
AloI	GAACNNNNNNTCC	2 cut(s)	818, 850
AluBI	AGCT	7 cut(s)	262, 328, 391, 1104, 1177, 1246, 1258
AluI	AGCT	7 cut(s)	262, 328, 391, 1104, 1177, 1246, 1258
Alw21I	GWGCWC	2 cut(s)	138, 330
Alw26I	GTCTC	5 cut(s)	493, 542, 788, 848, 1022
Alw44I	GTGCAC	1 cut(s)	134
AlwNI	CAGNNNCTG	1 cut(s)	391
Ama87I	CYCGRG	3 cut(s)	449, 563, 970
AoxI	GGCC	6 cut(s)	94, 102, 162, 201, 1223, 1250
ApaLI	GTGCAC	1 cut(s)	134
ApeKI	GCWGC	3 cut(s)	88, 388, 1255
ApoI	RAATTY	4 cut(s)	787, 860, 1135, 1195
ArsI	GACNNNNNNTTYG	2 cut(s)	818, 850
AsiGI	ACCGGT	1 cut(s)	1161
Asp718I	GGTACC	1 cut(s)	846
AspLEI	GCGC	6 cut(s)	47, 127, 234, 509, 577, 611
AspS9I	GGNCC	8 cut(s)	94, 102, 181, 202, 523, 558, 826, 1223
AsuC2I	CCSGG	4 cut(s)	564, 565, 851, 1281
AsuHPI	GGTGA	3 cut(s)	13, 706, 1099
AvaI	CYCGRG	3 cut(s)	449, 563, 970
AvaII	GGWCC	4 cut(s)	181, 523, 558, 826
BaeGI	GKGCMC	2 cut(s)	138, 246
BanI	GGYRCC	3 cut(s)	243, 574, 846
BanII	GRGCYC	1 cut(s)	330
BarI	GAAGNNNNNNTAC	2 cut(s)	584, 616
Bbv12I	GWGCWC	2 cut(s)	138, 330
BbvCI	CCTCAGC	1 cut(s)	1247
BbvI	GCAGC	3 cut(s)	75, 400, 1267
BccI	CCATC	2 cut(s)	732, 1141
BceAI	ACGGC	5 cut(s)	149, 219, 357, 386, 622
BciVI	GTATCC	1 cut(s)	401
BcnI	CCSGG	4 cut(s)	564, 565, 851, 1281
BcoDI	GTCTC	5 cut(s)	493, 542, 788, 848, 1022
BfoI	RGCGCY	2 cut(s)	510, 578
BfuAI	ACCTGC	1 cut(s)	511
BfuI	GTATCC	1 cut(s)	401
BglII	AGATCT	3 cut(s)	219, 291, 339
BisI	GCNGC	9 cut(s)	89, 97, 201, 322, 389, 634, 1226, 1253, 1256
BlpI	GCTNAGC	1 cut(s)	503
BlsI	GCNGC	9 cut(s)	90, 98, 202, 323, 390, 635, 1227, 1254, 1257
Bme1390I	CCNGG	4 cut(s)	564, 565, 851, 1281
Bme18I	GGWCC	4 cut(s)	181, 523, 558, 826
BmeRI	GACNNNNNGTC	2 cut(s)	625, 916
BmeT110I	CYCGRG	3 cut(s)	449, 563, 970
BmgBI	CACGTC	1 cut(s)	1081
BmgT120I	GGNCC	8 cut(s)	94, 102, 181, 202, 523, 558, 826, 1223
BmiI	GGNNCC	9 cut(s)	103, 245, 559, 560, 576, 732, 776, 828, 848
BmrFI	CCNGG	4 cut(s)	564, 565, 851, 1281
BmsI	GCATC	2 cut(s)	373, 645
Bpu10I	CCTNAGC	1 cut(s)	1247
Bpu1102I	GCTNAGC	1 cut(s)	503
BpuMI	CCSGG	4 cut(s)	564, 565, 851, 1281
BsaAI	YACGTR	1 cut(s)	571
BsaBI	GATNNNNATC	2 cut(s)	224, 620
BsaHI	GRCGYC	4 cut(s)	15, 575, 743, 1031
BsaI	GGTCTC	1 cut(s)	542
BsaJI	CCNNGG	6 cut(s)	97, 315, 345, 562, 563, 830
BsaWI	WCCGGW	2 cut(s)	525, 1161
BsaXI	ACNNNNNCTCC	2 cut(s)	1275, 1305
Bsc4I	CCNNNNNNNGG	6 cut(s)	351, 421, 798, 1005, 1168, 1295
Bse118I	RCCGGY	3 cut(s)	1161, 1288, 1295
Bse1I	ACTGG	1 cut(s)	479
Bse8I	GATNNNNATC	2 cut(s)	224, 620
BseDI	CCNNGG	6 cut(s)	97, 315, 345, 562, 563, 830
BseJI	GATNNNNATC	2 cut(s)	224, 620
BseLI	CCNNNNNNNGG	6 cut(s)	351, 421, 798, 1005, 1168, 1295
BseMII	CTCAG	3 cut(s)	382, 494, 1238
BseNI	ACTGG	1 cut(s)	479
BseRI	GAGGAG	4 cut(s)	452, 530, 767, 1073
BseSI	GKGCMC	2 cut(s)	138, 246
BseXI	GCAGC	3 cut(s)	75, 400, 1267
BseYI	CCCAGC	2 cut(s)	91, 1104
Bsh1285I	CGRYCG	2 cut(s)	1280, 1293
BshFI	GGCC	6 cut(s)	96, 104, 164, 203, 1225, 1252
BshNI	GGYRCC	3 cut(s)	243, 574, 846
BshTI	ACCGGT	1 cut(s)	1161
BsiEI	CGRYCG	2 cut(s)	1280, 1293
BsiHKAI	GWGCWC	2 cut(s)	138, 330
BsiHKCI	CYCGRG	3 cut(s)	449, 563, 970
BslFI	GGGAC	4 cut(s)	571, 911, 917, 1091
BslI	CCNNNNNNNGG	6 cut(s)	351, 421, 798, 1005, 1168, 1295
BsmAI	GTCTC	5 cut(s)	493, 542, 788, 848, 1022
BsmBI	CGTCTC	2 cut(s)	788, 1022
BsmFI	GGGAC	4 cut(s)	571, 911, 917, 1091
BsnI	GGCC	6 cut(s)	96, 104, 164, 203, 1225, 1252
Bso31I	GGTCTC	1 cut(s)	542
BsoBI	CYCGRG	3 cut(s)	449, 563, 970
Bsp1286I	GDGCHC	3 cut(s)	138, 246, 330
Bsp143I	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
Bsp1720I	GCTNAGC	1 cut(s)	503
Bsp68I	TCGCGA	2 cut(s)	110, 590
BspANI	GGCC	6 cut(s)	96, 104, 164, 203, 1225, 1252
BspCNI	CTCAG	3 cut(s)	381, 495, 1239
BspLI	GGNNCC	9 cut(s)	103, 245, 559, 560, 576, 732, 776, 828, 848
BspMI	ACCTGC	1 cut(s)	511
BspQI	GCTCTTC	2 cut(s)	335, 1094
BspT107I	GGYRCC	3 cut(s)	243, 574, 846
BspTNI	GGTCTC	1 cut(s)	542
BsrBI	CCGCTC	1 cut(s)	1012
BsrFI	RCCGGY	3 cut(s)	1161, 1288, 1295
BsrI	ACTGG	1 cut(s)	479
BssAI	RCCGGY	3 cut(s)	1161, 1288, 1295
BssECI	CCNNGG	6 cut(s)	97, 315, 345, 562, 563, 830
BssMI	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
BssNI	GRCGYC	4 cut(s)	15, 575, 743, 1031
BssT1I	CCWWGG	2 cut(s)	315, 345
Bst4CI	ACNGT	4 cut(s)	536, 808, 1265, 1304
Bst6I	CTCTTC	2 cut(s)	335, 1094
BstACI	GRCGYC	4 cut(s)	15, 575, 743, 1031
BstBAI	YACGTR	1 cut(s)	571
BstC8I	GCNNGC	4 cut(s)	173, 326, 362, 661
BstDEI	CTNAG	4 cut(s)	282, 368, 503, 1247
BstDSI	CCRYGG	2 cut(s)	97, 830
BstH2I	RGCGCY	2 cut(s)	510, 578
BstHHI	GCGC	6 cut(s)	47, 127, 234, 509, 577, 611
BstKTI	GATC	7 cut(s)	12, 159, 222, 294, 342, 396, 618
BstMAI	GTCTC	5 cut(s)	493, 542, 788, 848, 1022
BstMBI	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
BstMCI	CGRYCG	2 cut(s)	1280, 1293
BstMWI	GCNNNNNNNGC	5 cut(s)	133, 142, 161, 1118, 1252
BstNSI	RCATGY	1 cut(s)	895
BstSCI	CCNGG	4 cut(s)	562, 563, 849, 1279
BstSLI	GKGCMC	2 cut(s)	138, 246
BstV1I	GCAGC	3 cut(s)	75, 400, 1267
BstX2I	RGATCY	3 cut(s)	219, 291, 339
BstYI	RGATCY	3 cut(s)	219, 291, 339
BsuI	GTATCC	1 cut(s)	401
BsuRI	GGCC	6 cut(s)	96, 104, 164, 203, 1225, 1252
BtgI	CCRYGG	2 cut(s)	97, 830
BtrI	CACGTC	1 cut(s)	1081
BtuMI	TCGCGA	2 cut(s)	110, 590
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	4 cut(s)	173, 326, 362, 661
CaiI	CAGNNNCTG	1 cut(s)	391
CfoI	GCGC	6 cut(s)	47, 127, 234, 509, 577, 611
Cfr10I	RCCGGY	3 cut(s)	1161, 1288, 1295
Cfr13I	GGNCC	8 cut(s)	94, 102, 181, 202, 523, 558, 826, 1223
Cfr42I	CCGCGG	1 cut(s)	100
Cfr9I	CCCGGG	1 cut(s)	563
CseI	GACGC	2 cut(s)	805, 1039
Csp6I	GTAC	9 cut(s)	73, 118, 379, 430, 568, 601, 700, 847, 1273
CspAI	ACCGGT	1 cut(s)	1161
CviAII	CATG	4 cut(s)	229, 304, 691, 892
CviQI	GTAC	9 cut(s)	73, 118, 379, 430, 568, 601, 700, 847, 1273
DdeI	CTNAG	4 cut(s)	282, 368, 503, 1247
DinI	GGCGCC	1 cut(s)	576
DpnI	GATC	7 cut(s)	11, 158, 221, 293, 341, 395, 617
DpnII	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
DraIII	CACNNNGTG	1 cut(s)	256
DriI	GACNNNNNGTC	2 cut(s)	625, 916
Eam1104I	CTCTTC	2 cut(s)	335, 1094
Eam1105I	GACNNNNNGTC	2 cut(s)	625, 916
EarI	CTCTTC	2 cut(s)	335, 1094
EciI	GGCGGA	2 cut(s)	388, 1040
Ecl136II	GAGCTC	1 cut(s)	328
Eco130I	CCWWGG	2 cut(s)	315, 345
Eco24I	GRGCYC	1 cut(s)	330
Eco31I	GGTCTC	1 cut(s)	542
Eco32I	GATATC	2 cut(s)	967, 996
Eco47I	GGWCC	4 cut(s)	181, 523, 558, 826
Eco53kI	GAGCTC	1 cut(s)	328
Eco57I	CTGAAG	2 cut(s)	206, 776
Eco88I	CYCGRG	3 cut(s)	449, 563, 970
EcoICRI	GAGCTC	1 cut(s)	328
EcoO109I	RGGNCCY	4 cut(s)	102, 181, 558, 826
EcoRI	GAATTC	1 cut(s)	787
EcoRV	GATATC	2 cut(s)	967, 996
EcoT14I	CCWWGG	2 cut(s)	315, 345
EcoT38I	GRGCYC	1 cut(s)	330
EgeI	GGCGCC	1 cut(s)	576
EheI	GGCGCC	1 cut(s)	576
ErhI	CCWWGG	2 cut(s)	315, 345
Esp3I	CGTCTC	2 cut(s)	788, 1022
FaeI	CATG	4 cut(s)	232, 307, 694, 895
FaiI	YATR	5 cut(s)	230, 305, 692, 893, 1234
FalI	AAGNNNNNCTT	2 cut(s)	281, 313
FaqI	GGGAC	4 cut(s)	571, 911, 917, 1091
FatI	CATG	4 cut(s)	228, 303, 690, 891
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	9 cut(s)	89, 97, 201, 322, 389, 634, 1226, 1253, 1256
FriOI	GRGCYC	1 cut(s)	330
Fsp4HI	GCNGC	9 cut(s)	89, 97, 201, 322, 389, 634, 1226, 1253, 1256
GlaI	GCGC	6 cut(s)	46, 126, 233, 508, 576, 610
GluI	GCNGC	9 cut(s)	89, 97, 201, 322, 389, 634, 1226, 1253, 1256
GsaI	CCCAGC	2 cut(s)	95, 1108
HaeII	RGCGCY	2 cut(s)	510, 578
HaeIII	GGCC	6 cut(s)	96, 104, 164, 203, 1225, 1252
HgaI	GACGC	2 cut(s)	805, 1039
HhaI	GCGC	6 cut(s)	47, 127, 234, 509, 577, 611
Hin1I	GRCGYC	4 cut(s)	15, 575, 743, 1031
Hin1II	CATG	4 cut(s)	232, 307, 694, 895
Hin6I	GCGC	6 cut(s)	45, 125, 232, 507, 575, 609
HinP1I	GCGC	6 cut(s)	45, 125, 232, 507, 575, 609
HincII	GTYRAC	3 cut(s)	58, 811, 1167
HindII	GTYRAC	3 cut(s)	58, 811, 1167
HinfI	GANTC	4 cut(s)	38, 454, 583, 685
HpaI	GTTAAC	2 cut(s)	58, 1167
HphI	GGTGA	3 cut(s)	13, 706, 1099
Hpy166II	GTNNAC	6 cut(s)	58, 118, 136, 430, 811, 1167
Hpy188I	TCNGA	7 cut(s)	407, 493, 532, 654, 735, 793, 1000
Hpy188III	TCNNGA	8 cut(s)	67, 109, 184, 256, 334, 589, 838, 1144
Hpy8I	GTNNAC	6 cut(s)	58, 118, 136, 430, 811, 1167
HpyAV	CCTTC	8 cut(s)	106, 115, 181, 589, 817, 835, 1045, 1063
HpyCH4III	ACNGT	4 cut(s)	536, 808, 1265, 1304
HpyCH4IV	ACGT	7 cut(s)	15, 132, 207, 570, 743, 917, 1080
HpyCH4V	TGCA	1 cut(s)	136
HpyF10VI	GCNNNNNNNGC	5 cut(s)	133, 142, 161, 1118, 1252
HpyF3I	CTNAG	4 cut(s)	282, 368, 503, 1247
HpySE526I	ACGT	7 cut(s)	15, 132, 207, 570, 743, 917, 1080
Hsp92I	GRCGYC	4 cut(s)	15, 575, 743, 1031
Hsp92II	CATG	4 cut(s)	232, 307, 694, 895
HspAI	GCGC	6 cut(s)	45, 125, 232, 507, 575, 609
KasI	GGCGCC	1 cut(s)	574
KflI	GGGWCCC	1 cut(s)	558
KpnI	GGTACC	1 cut(s)	850
KspAI	GTTAAC	2 cut(s)	58, 1167
KspI	CCGCGG	1 cut(s)	100
Kzo9I	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
LguI	GCTCTTC	2 cut(s)	335, 1094
LmnI	GCTCC	5 cut(s)	142, 465, 517, 780, 1009
Lsp1109I	GCAGC	3 cut(s)	75, 400, 1267
LweI	GCATC	2 cut(s)	373, 645
MaeII	ACGT	7 cut(s)	15, 132, 207, 570, 743, 917, 1080
MaeIII	GTNAC	3 cut(s)	19, 1087, 1298
MalI	GATC	7 cut(s)	11, 158, 221, 293, 341, 395, 617
MbiI	CCGCTC	1 cut(s)	1012
MboI	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
MboII	GAAGA	7 cut(s)	322, 349, 604, 625, 1108, 1111, 1138
MflI	RGATCY	3 cut(s)	219, 291, 339
MhlI	GDGCHC	3 cut(s)	138, 246, 330
MluCI	AATT	5 cut(s)	787, 860, 1115, 1135, 1195
MluI	ACGCGT	1 cut(s)	930
Mly113I	GGCGCC	1 cut(s)	575
MlyI	GAGTC	1 cut(s)	32
MmeI	TCCRAC	2 cut(s)	385, 1023
MseI	TTAA	5 cut(s)	57, 210, 675, 1166, 1199
MslI	CAYNNNNRTG	1 cut(s)	695
MspA1I	CMGCKG	2 cut(s)	99, 391
MspR9I	CCNGG	4 cut(s)	564, 565, 851, 1281
MwoI	GCNNNNNNNGC	5 cut(s)	133, 142, 161, 1118, 1252
NarI	GGCGCC	1 cut(s)	575
NciI	CCSGG	4 cut(s)	564, 565, 851, 1281
NdeII	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
NlaIII	CATG	4 cut(s)	232, 307, 694, 895
NlaIV	GGNNCC	9 cut(s)	103, 245, 559, 560, 576, 732, 776, 828, 848
NmeAIII	GCCGAG	1 cut(s)	751
NmuCI	GTSAC	3 cut(s)	19, 1087, 1298
NruI	TCGCGA	2 cut(s)	110, 590
NspI	RCATGY	1 cut(s)	895
PciSI	GCTCTTC	2 cut(s)	335, 1094
PcsI	WCGNNNNNNNCGW	5 cut(s)	21, 72, 129, 906, 929
PfeI	GAWTC	3 cut(s)	454, 583, 685
PflFI	GACNNNGTC	1 cut(s)	17
PinAI	ACCGGT	1 cut(s)	1161
PkrI	GCNGC	9 cut(s)	90, 98, 202, 323, 390, 635, 1227, 1254, 1257
PleI	GAGTC	1 cut(s)	32
PluTI	GGCGCC	1 cut(s)	578
PpsI	GAGTC	1 cut(s)	32
Ppu21I	YACGTR	1 cut(s)	571
PpuMI	RGGWCCY	3 cut(s)	181, 558, 826
Psp124BI	GAGCTC	1 cut(s)	330
Psp5II	RGGWCCY	3 cut(s)	181, 558, 826
PspFI	CCCAGC	2 cut(s)	91, 1104
PspN4I	GGNNCC	9 cut(s)	103, 245, 559, 560, 576, 732, 776, 828, 848
PspPI	GGNCC	8 cut(s)	94, 102, 181, 202, 523, 558, 826, 1223
PspPPI	RGGWCCY	3 cut(s)	181, 558, 826
PsrI	GAACNNNNNNTAC	2 cut(s)	647, 679
PstNI	CAGNNNCTG	1 cut(s)	391
PsuI	RGATCY	3 cut(s)	219, 291, 339
PsyI	GACNNNGTC	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	391
RruI	TCGCGA	2 cut(s)	110, 590
RsaI	GTAC	9 cut(s)	74, 119, 380, 431, 569, 602, 701, 848, 1274
RsaNI	GTAC	9 cut(s)	73, 118, 379, 430, 568, 601, 700, 847, 1273
RseI	CAYNNNNRTG	1 cut(s)	695
SacI	GAGCTC	1 cut(s)	330
SacII	CCGCGG	1 cut(s)	100
SalI	GTCGAC	1 cut(s)	809
SapI	GCTCTTC	2 cut(s)	335, 1094
SaqAI	TTAA	5 cut(s)	57, 210, 675, 1166, 1199
SatI	GCNGC	9 cut(s)	89, 97, 201, 322, 389, 634, 1226, 1253, 1256
Sau3AI	GATC	7 cut(s)	9, 156, 219, 291, 339, 393, 615
Sau96I	GGNCC	8 cut(s)	94, 102, 181, 202, 523, 558, 826, 1223
SchI	GAGTC	1 cut(s)	32
ScrFI	CCNGG	4 cut(s)	564, 565, 851, 1281
SduI	GDGCHC	3 cut(s)	138, 246, 330
SfaNI	GCATC	2 cut(s)	373, 645
SfoI	GGCGCC	1 cut(s)	576
Sfr303I	CCGCGG	1 cut(s)	100
SgrAI	CRCCGGYG	1 cut(s)	1295
SgrBI	CCGCGG	1 cut(s)	100
SgrDI	CGTCGACG	1 cut(s)	809
SinI	GGWCC	4 cut(s)	181, 523, 558, 826
SmaI	CCCGGG	1 cut(s)	565
SmiMI	CAYNNNNRTG	1 cut(s)	695
Sse9I	AATT	5 cut(s)	787, 860, 1115, 1135, 1195
SspDI	GGCGCC	1 cut(s)	574
SstI	GAGCTC	1 cut(s)	330
StyD4I	CCNGG	4 cut(s)	562, 563, 849, 1279
StyI	CCWWGG	2 cut(s)	315, 345
TaaI	ACNGT	4 cut(s)	536, 808, 1265, 1304
TaiI	ACGT	7 cut(s)	18, 135, 210, 573, 746, 920, 1083
TaqI	TCGA	9 cut(s)	12, 465, 618, 780, 810, 837, 864, 870, 963
TasI	AATT	5 cut(s)	787, 860, 1115, 1135, 1195
TauI	GCSGC	6 cut(s)	99, 203, 324, 636, 1228, 1255
TfiI	GAWTC	3 cut(s)	454, 583, 685
Tru1I	TTAA	5 cut(s)	57, 210, 675, 1166, 1199
Tru9I	TTAA	5 cut(s)	57, 210, 675, 1166, 1199
TseFI	GTSAC	3 cut(s)	19, 1087, 1298
TseI	GCWGC	3 cut(s)	88, 388, 1255
Tsp45I	GTSAC	3 cut(s)	19, 1087, 1298
TspDTI	ATGAA	1 cut(s)	289
TspGWI	ACGGA	4 cut(s)	155, 530, 1106, 1184
TspMI	CCCGGG	1 cut(s)	563
Tth111I	GACNNNGTC	1 cut(s)	17
VneI	GTGCAC	1 cut(s)	134
VpaK11BI	GGWCC	4 cut(s)	181, 523, 558, 826
XapI	RAATTY	4 cut(s)	787, 860, 1135, 1195
XceI	RCATGY	1 cut(s)	895
XmaI	CCCGGG	1 cut(s)	563
XmiI	GTMKAC	1 cut(s)	810
ZraI	GACGTC	2 cut(s)	16, 744

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.