Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009014

Physical Location & Seq

Genomic Coordinates Reverse (-)

26423098 .. 26427141

Gene Structure 4044 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1172700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGAACAAGAACAACAGCCGGATCGGAAGAAGGTCTGCCATGGGAGGCAGGCCACAGTCACCTGCATACGTTGAAACAGATCCATCAGGTCGATACGGACGATTCAGGGAAATGCTTGGCAAAGGAGCCATGAAGACAGTTTACAAGGCATTTGATGAGGTCCTCGGAATGGAGGTGGCGTGGAATCAAGTCAAGCTTAATGATGCCTTCAGTTCGCCGGACGAACTTCAGCGCCTATACTCCGAGGTCCACCTCCTCAAGAACCTGAAGCATGACTCCATCATCCAATACTACAAGTCGTGGATCGATGTTAATCATCGAACCTTCAACTTCATCACCGAAATGTTCACCTCCGGCACTCTCAGAGAGTACAGACATAAGTACCAGCGAGTGAATATTGGTGCAGTGAAAAATTGGTCCCGGCAGATCTTACGTGGTCTGGCTTATTTGCACAAACTTTATCCACCTGTGATTCACAGAGACCTCAAGTGTGACAACATCTTTGTTAATGGCCATCTAGGGCAAGTCAAGATTGGTGATTTGGGCTTGGCTGCTATTCTTCGTGGTACACAACACGCCCACAGTGTCATAGGTACACCAGAATTTATGGCGCCTGAATTGTACGATGAGGAATACAATGAGCTAGTGGACATATATGCCTTTGGCATGTGTGTGTTGGAAATGCTTACTTCAGAATATCCCTATAGCGAGTGCTCAAATCCTGCCCAAATTTACAAGAAAGTTACTTCAGGGAAGCTACCAAACGCATTCTACAAGATTGAAGATTTGGAGGCTCAACGATTTGTAGGGAAGTGTCTGGTGAATGTTTCAAAGAGGTTACCGGCACACAAGCTATTGTTAGACCCTTTTCTAGCCTCTGATGATGTAGAGCTAGCGTCCCCTCCAAGAATCTCAATTCCCAAGTTAATTCCTAATGATGATCCAGTGGCGGAGGCGGGGGATGAGGAGGATGAGCTACATTTTTCCGCTGAGGTTGATTTGGAAAGAAGCACAAACATGATCATCACTGGAAAAATGAATCCAGAGGATGATACTATTTCTCTCACAGTTCGGATTTCTGATCTGGACGGGAAAAATGCTAGGAACATATACTTTCCCTTTGACATCATGAATGACACTGCAATTGATGTAGCAACGGAGATGGTGAAAGAATTAGAAATCAGTGACTGGGAACCACCAGAGATCGCAGGGATGATAGAAAAGGAGATATTTTCTTTGATTCCGGGTCGCAAGAAATGGGGCTCACCCCAAGATTATCATCCTCATCAGCACAGCTTTAACTATGAAGAAGAGGATGACGATGATGGTGGTTTCAACCATCATCCTTTCTACTCATTCTCTTCGTGCTCTTCATCCCAAAATTCTCTTCATTCTTTCAGATCTCACTGCAAGAATCATAATTTGCTTCACGGTGATTTGTTTATGAATGATGATGTAAGTTCTCAGAGTTCATTCGACTCCTGCAACTACTCCAACATAAATTACTACTCAGGCAATGAAGATGATCTCCATGACGTGAGCTTCGGAAAAGCTACTCATAAATCTACTCGGTTTTGTCCTTCAGAAAGCAGGGGTGCAAATTCTTACAAACAATGCAATTCACAACTAGATAACCAGAGACCTCACAAATTGAATCATCATCAGGAAAGGAAATTACCCAAGATTCAATCACTCATCGACGTGCGCAGTCAATTGTTGCACCGGTCACTGGAGGAGGAGGTAAACAAGAGGCGGTTGTTTAAGACTGTTGGGACAATTGAGAACGTTGGGTTTCAAACACCAGGAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

604

Amino Acids

68.91

Weight (kDa)

5.86

Isoelectric Point (pI)

52.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	35 - 254	2.4e-29	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	37 - 291	1.2e-45	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009014)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51630 AT3G51630
fragaria_vesca	FvH4_6g10120
malus_domestica	MD04G1172700.v1.1 MD12G1186000.v1.1
prunus_persica	Prupe.4G073500_v2.0.a1 Prupe.6G291500_v2.0.a1
pyrus_communis	pycom04g15260 pycom12g17460
rosa_chinensis	RchiOBHm_Chr3g0460821
rosa_laevigata	RLG00000024978
rosa_multiflora	Rmu_sc0007678.1_g000013
rosa_roxburghii	Rroxscaffold_6G00419190
rosa_rugosa	Rorug03G0046600 Rorug03G0046700 Rorug03G0046800 Rorug03G0046800
rosa_samantha	Rh3AG103700 Rh3BG107400 Rh3CG109300 Rh3DG109200
rosa_wichuraiana	Rw3G008830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	70
Acc16I	TGCGCA	1 cut(s)	1706
Acc36I	ACCTGC	1 cut(s)	70
AccB1I	GGYRCC	1 cut(s)	610
AciI	CCGC	4 cut(s)	950, 956, 987, 1753
AclI	AACGTT	1 cut(s)	1785
AclWI	GGATC	4 cut(s)	29, 74, 311, 935
AcoI	YGGCCR	1 cut(s)	511
AcsI	RAATTY	4 cut(s)	602, 729, 1381, 1600
AcuI	CTGAAG	6 cut(s)	193, 212, 287, 675, 732, 1566
AcyI	GRCGYC	1 cut(s)	611
AfaI	GTAC	5 cut(s)	371, 383, 568, 595, 623
AfiI	CCNNNNNNNGG	2 cut(s)	169, 1729
AgeI	ACCGGT	1 cut(s)	1722
AgsI	TTSAA	8 cut(s)	74, 328, 782, 831, 1336, 1654, 1688, 1796
AjiI	CACGTC	2 cut(s)	1537, 1702
AjnI	CCWGG	1 cut(s)	1801
AjuI	GAANNNNNNNTTGG	2 cut(s)	1371, 1403
Alw21I	GWGCWC	2 cut(s)	716, 1370
Alw26I	GTCTC	2 cut(s)	474, 1633
AlwI	GGATC	4 cut(s)	29, 74, 311, 935
AlwNI	CAGNNNCTG	1 cut(s)	1188
AoxI	GGCC	2 cut(s)	50, 511
ApeKI	GCWGC	1 cut(s)	551
ApoI	RAATTY	4 cut(s)	602, 729, 1381, 1600
ArsI	GACNNNNNNTTYG	4 cut(s)	485, 517, 1526, 1558
AsiGI	ACCGGT	1 cut(s)	1722
AspLEI	GCGC	3 cut(s)	234, 613, 1707
AspS9I	GGNCC	3 cut(s)	160, 247, 417
AsuC2I	CCSGG	2 cut(s)	421, 1245
AsuHPI	GGTGA	8 cut(s)	51, 328, 340, 548, 832, 1177, 1257, 1445
AsuNHI	GCTAGC	1 cut(s)	892
AvaII	GGWCC	3 cut(s)	160, 247, 417
BaeI	ACNNNNGTAYC	2 cut(s)	365, 398
BalI	TGGCCA	1 cut(s)	513
BanI	GGYRCC	1 cut(s)	610
BanII	GRGCYC	1 cut(s)	1265
BarI	GAAGNNNNNNTAC	2 cut(s)	125, 157
BbsI	GAAGAC	1 cut(s)	140
Bbv12I	GWGCWC	2 cut(s)	716, 1370
BbvCI	CCTCAGC	1 cut(s)	990
BbvI	GCAGC	1 cut(s)	538
BccI	CCATC	6 cut(s)	91, 287, 522, 1156, 1319, 1347
BciT130I	CCWGG	1 cut(s)	1803
BclI	TGATCA	1 cut(s)	1020
BcnI	CCSGG	2 cut(s)	421, 1245
BcoDI	GTCTC	2 cut(s)	474, 1633
BfaI	CTAG	7 cut(s)	518, 644, 872, 893, 1101, 1628, 1810
BfmI	CTRYAG	1 cut(s)	703
BfoI	RGCGCY	2 cut(s)	235, 614
BfuAI	ACCTGC	1 cut(s)	70
BglII	AGATCT	2 cut(s)	426, 1400
BisI	GCNGC	1 cut(s)	552
BlsI	GCNGC	1 cut(s)	553
Bme1390I	CCNGG	3 cut(s)	421, 1245, 1803
Bme18I	GGWCC	3 cut(s)	160, 247, 417
BmgBI	CACGTC	2 cut(s)	1537, 1702
BmgT120I	GGNCC	3 cut(s)	160, 247, 417
BmiI	GGNNCC	4 cut(s)	127, 419, 612, 1194
BmrFI	CCNGG	3 cut(s)	421, 1245, 1803
BmrI	ACTGGG	1 cut(s)	1198
BmsI	GCATC	1 cut(s)	193
BmtI	GCTAGC	1 cut(s)	896
BmuI	ACTGGG	1 cut(s)	1198
BpiI	GAAGAC	1 cut(s)	140
BpmI	CTGGAG	1 cut(s)	1751
Bpu10I	CCTNAGC	1 cut(s)	990
BpuEI	CTTGAG	2 cut(s)	242, 470
BpuMI	CCSGG	2 cut(s)	421, 1245
Bsa29I	ATCGAT	1 cut(s)	306
BsaAI	YACGTR	1 cut(s)	434
BsaBI	GATNNNNATC	2 cut(s)	312, 1278
BsaHI	GRCGYC	1 cut(s)	611
BsaI	GGTCTC	2 cut(s)	474, 1633
BsaJI	CCNNGG	3 cut(s)	39, 163, 243
BsaWI	WCCGGW	1 cut(s)	1722
BsaXI	ACNNNNNCTCC	2 cut(s)	164, 194
Bsc4I	CCNNNNNNNGG	2 cut(s)	169, 1729
Bse118I	RCCGGY	2 cut(s)	841, 1722
Bse1I	ACTGG	4 cut(s)	944, 1033, 1193, 1734
Bse3DI	GCAATG	1 cut(s)	1522
Bse8I	GATNNNNATC	2 cut(s)	312, 1278
BseBI	CCWGG	1 cut(s)	1803
BseCI	ATCGAT	1 cut(s)	306
BseDI	CCNNGG	3 cut(s)	39, 163, 243
BseGI	GGATG	9 cut(s)	282, 967, 976, 1054, 1218, 1279, 1321, 1342, 1373
BseJI	GATNNNNATC	2 cut(s)	312, 1278
BseLI	CCNNNNNNNGG	2 cut(s)	169, 1729
BseMI	GCAATG	1 cut(s)	1522
BseMII	CTCAG	4 cut(s)	376, 981, 1478, 1524
BseNI	ACTGG	4 cut(s)	944, 1033, 1193, 1734
BseRI	GAGGAG	4 cut(s)	245, 980, 1748, 1751
BseXI	GCAGC	1 cut(s)	538
BsgI	GTGCAG	1 cut(s)	423
BshFI	GGCC	2 cut(s)	52, 513
BshNI	GGYRCC	1 cut(s)	610
BshTI	ACCGGT	1 cut(s)	1722
BshVI	ATCGAT	1 cut(s)	306
BsiHKAI	GWGCWC	2 cut(s)	716, 1370
BsiSI	CCGG	7 cut(s)	19, 218, 354, 421, 842, 1244, 1723
BslFI	GGGAC	3 cut(s)	403, 883, 1786
BslI	CCNNNNNNNGG	2 cut(s)	169, 1729
BsmAI	GTCTC	2 cut(s)	474, 1633
BsmFI	GGGAC	3 cut(s)	403, 883, 1786
BsmI	GAATGC	1 cut(s)	767
BsnI	GGCC	2 cut(s)	52, 513
Bso31I	GGTCTC	2 cut(s)	474, 1633
Bsp1286I	GDGCHC	3 cut(s)	716, 1265, 1370
Bsp19I	CCATGG	1 cut(s)	39
BspACI	CCGC	4 cut(s)	950, 956, 987, 1753
BspANI	GGCC	2 cut(s)	52, 513
BspCNI	CTCAG	4 cut(s)	375, 982, 1477, 1523
BspDI	ATCGAT	1 cut(s)	306
BspHI	TCATGA	1 cut(s)	1128
BspLI	GGNNCC	4 cut(s)	127, 419, 612, 1194
BspMI	ACCTGC	1 cut(s)	70
BspOI	GCTAGC	1 cut(s)	896
BspPI	GGATC	4 cut(s)	29, 74, 311, 935
BspQI	GCTCTTC	1 cut(s)	1375
BspT107I	GGYRCC	1 cut(s)	610
BspTNI	GGTCTC	2 cut(s)	474, 1633
BsrDI	GCAATG	1 cut(s)	1522
BsrFI	RCCGGY	2 cut(s)	841, 1722
BsrI	ACTGG	4 cut(s)	944, 1033, 1193, 1734
BssAI	RCCGGY	2 cut(s)	841, 1722
BssECI	CCNNGG	3 cut(s)	39, 163, 243
BssNI	GRCGYC	1 cut(s)	611
BssT1I	CCWWGG	1 cut(s)	39
Bst2UI	CCWGG	1 cut(s)	1803
Bst4CI	ACNGT	6 cut(s)	57, 139, 584, 1069, 1433, 1768
Bst6I	CTCTTC	4 cut(s)	1305, 1366, 1375, 1392
BstACI	GRCGYC	1 cut(s)	611
BstBAI	YACGTR	1 cut(s)	434
BstC8I	GCNNGC	2 cut(s)	50, 894
BstDEI	CTNAG	4 cut(s)	362, 990, 1464, 1510
BstDSI	CCRYGG	1 cut(s)	39
BstEII	GGTNACC	1 cut(s)	837
BstF5I	GGATG	9 cut(s)	282, 967, 976, 1054, 1218, 1279, 1321, 1342, 1373
BstH2I	RGCGCY	2 cut(s)	235, 614
BstHHI	GCGC	3 cut(s)	234, 613, 1707
BstMAI	GTCTC	2 cut(s)	474, 1633
BstNI	CCWGG	1 cut(s)	1803
BstNSI	RCATGY	1 cut(s)	670
BstPI	GGTNACC	1 cut(s)	837
BstSCI	CCNGG	3 cut(s)	419, 1243, 1801
BstSFI	CTRYAG	1 cut(s)	703
BstV1I	GCAGC	1 cut(s)	538
BstV2I	GAAGAC	1 cut(s)	140
BstX2I	RGATCY	3 cut(s)	79, 426, 1400
BstYI	RGATCY	3 cut(s)	79, 426, 1400
Bsu15I	ATCGAT	1 cut(s)	306
BsuRI	GGCC	2 cut(s)	52, 513
BsuTUI	ATCGAT	1 cut(s)	306
BtgI	CCRYGG	1 cut(s)	39
BtrI	CACGTC	2 cut(s)	1537, 1702
BtsCI	GGATG	9 cut(s)	282, 967, 976, 1054, 1218, 1279, 1321, 1342, 1373
BtsI	GCAGTG	3 cut(s)	411, 1137, 1405
BtsIMutI	CAGTG	8 cut(s)	411, 589, 951, 1026, 1137, 1189, 1405, 1727
BveI	ACCTGC	1 cut(s)	70
Cac8I	GCNNGC	2 cut(s)	50, 894
CaiI	CAGNNNCTG	1 cut(s)	1188
CciI	TCATGA	1 cut(s)	1128
CfoI	GCGC	3 cut(s)	234, 613, 1707
Cfr10I	RCCGGY	2 cut(s)	841, 1722
Cfr13I	GGNCC	3 cut(s)	160, 247, 417
ClaI	ATCGAT	1 cut(s)	306
CseI	GACGC	1 cut(s)	885
Csp6I	GTAC	5 cut(s)	370, 382, 567, 594, 622
CspAI	ACCGGT	1 cut(s)	1722
CviAII	CATG	7 cut(s)	40, 130, 272, 667, 1018, 1129, 1532
CviQI	GTAC	5 cut(s)	370, 382, 567, 594, 622
DdeI	CTNAG	4 cut(s)	362, 990, 1464, 1510
DinI	GGCGCC	1 cut(s)	612
EaeI	YGGCCR	1 cut(s)	511
Eam1104I	CTCTTC	4 cut(s)	1305, 1366, 1375, 1392
EarI	CTCTTC	4 cut(s)	1305, 1366, 1375, 1392
EciI	GGCGGA	1 cut(s)	965
Eco130I	CCWWGG	1 cut(s)	39
Eco24I	GRGCYC	1 cut(s)	1265
Eco31I	GGTCTC	2 cut(s)	474, 1633
Eco47I	GGWCC	3 cut(s)	160, 247, 417
Eco57I	CTGAAG	6 cut(s)	193, 212, 287, 675, 732, 1566
Eco91I	GGTNACC	1 cut(s)	837
EcoO109I	RGGNCCY	1 cut(s)	160
EcoO65I	GGTNACC	1 cut(s)	837
EcoRII	CCWGG	1 cut(s)	1801
EcoT14I	CCWWGG	1 cut(s)	39
EcoT38I	GRGCYC	1 cut(s)	1265
EgeI	GGCGCC	1 cut(s)	612
EheI	GGCGCC	1 cut(s)	612
ErhI	CCWWGG	1 cut(s)	39
FaeI	CATG	7 cut(s)	43, 133, 275, 670, 1021, 1132, 1535
FalI	AAGNNNNNCTT	2 cut(s)	180, 212
FaqI	GGGAC	3 cut(s)	403, 883, 1786
FatI	CATG	7 cut(s)	39, 129, 271, 666, 1017, 1128, 1531
FauI	CCCGC	1 cut(s)	949
FbaI	TGATCA	1 cut(s)	1020
Fnu4HI	GCNGC	1 cut(s)	552
FokI	GGATG	9 cut(s)	269, 974, 983, 1061, 1225, 1266, 1328, 1329, 1360
FriOI	GRGCYC	1 cut(s)	1265
Fsp4HI	GCNGC	1 cut(s)	552
FspBI	CTAG	7 cut(s)	518, 644, 872, 893, 1101, 1628, 1810
FspI	TGCGCA	1 cut(s)	1706
GlaI	GCGC	3 cut(s)	233, 612, 1706
GluI	GCNGC	1 cut(s)	552
GsuI	CTGGAG	1 cut(s)	1751
HaeII	RGCGCY	2 cut(s)	235, 614
HaeIII	GGCC	2 cut(s)	52, 513
HapII	CCGG	7 cut(s)	19, 218, 354, 421, 842, 1244, 1723
HgaI	GACGC	1 cut(s)	885
HhaI	GCGC	3 cut(s)	234, 613, 1707
Hin1I	GRCGYC	1 cut(s)	611
Hin1II	CATG	7 cut(s)	43, 133, 275, 670, 1021, 1132, 1535
Hin6I	GCGC	3 cut(s)	232, 611, 1705
HinP1I	GCGC	3 cut(s)	232, 611, 1705
HindIII	AAGCTT	1 cut(s)	194
HpaII	CCGG	7 cut(s)	19, 218, 354, 421, 842, 1244, 1723
HphI	GGTGA	8 cut(s)	51, 328, 340, 548, 832, 1177, 1257, 1445
Hpy166II	GTNNAC	7 cut(s)	142, 250, 348, 569, 596, 649, 1744
Hpy188III	TCNNGA	6 cut(s)	259, 529, 1043, 1085, 1129, 1664
Hpy8I	GTNNAC	7 cut(s)	142, 250, 348, 569, 596, 649, 1744
Hpy99I	CGWCG	1 cut(s)	1703
HpyAV	CCTTC	4 cut(s)	24, 217, 334, 1590
HpyCH4III	ACNGT	6 cut(s)	57, 139, 584, 1069, 1433, 1768
HpyCH4IV	ACGT	5 cut(s)	69, 433, 1536, 1701, 1785
HpyCH4V	TGCA	9 cut(s)	65, 404, 451, 1142, 1410, 1485, 1598, 1617, 1720
HpyF3I	CTNAG	4 cut(s)	362, 990, 1464, 1510
HpySE526I	ACGT	5 cut(s)	69, 433, 1536, 1701, 1785
Hsp92I	GRCGYC	1 cut(s)	611
Hsp92II	CATG	7 cut(s)	43, 133, 275, 670, 1021, 1132, 1535
HspAI	GCGC	3 cut(s)	232, 611, 1705
KasI	GGCGCC	1 cut(s)	610
Ksp22I	TGATCA	1 cut(s)	1020
LguI	GCTCTTC	1 cut(s)	1375
LmnI	GCTCC	1 cut(s)	125
Lsp1109I	GCAGC	1 cut(s)	538
LweI	GCATC	1 cut(s)	193
MaeI	CTAG	7 cut(s)	518, 644, 872, 893, 1101, 1628, 1810
MaeII	ACGT	5 cut(s)	69, 433, 1536, 1701, 1785
MaeIII	GTNAC	6 cut(s)	57, 491, 742, 837, 1184, 1725
MfeI	CAATTG	3 cut(s)	1143, 1712, 1776
MflI	RGATCY	3 cut(s)	79, 426, 1400
MhlI	GDGCHC	3 cut(s)	716, 1265, 1370
MlsI	TGGCCA	1 cut(s)	513
MluNI	TGGCCA	1 cut(s)	513
Mly113I	GGCGCC	1 cut(s)	611
MlyI	GAGTC	2 cut(s)	269, 1472
MmeI	TCCRAC	2 cut(s)	657, 1518
Mox20I	TGGCCA	1 cut(s)	513
MscI	TGGCCA	1 cut(s)	513
MseI	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1761
MslI	CAYNNNNRTG	4 cut(s)	341, 671, 1016, 1700
Msp20I	TGGCCA	1 cut(s)	513
MspA1I	CMGCKG	1 cut(s)	989
MspI	CCGG	7 cut(s)	19, 218, 354, 421, 842, 1244, 1723
MspR9I	CCNGG	3 cut(s)	421, 1245, 1803
MunI	CAATTG	3 cut(s)	1143, 1712, 1776
Mva1269I	GAATGC	1 cut(s)	767
MvaI	CCWGG	1 cut(s)	1803
NarI	GGCGCC	1 cut(s)	611
NciI	CCSGG	2 cut(s)	421, 1245
NcoI	CCATGG	1 cut(s)	39
NheI	GCTAGC	1 cut(s)	892
NlaIII	CATG	7 cut(s)	43, 133, 275, 670, 1021, 1132, 1535
NlaIV	GGNNCC	4 cut(s)	127, 419, 612, 1194
NmuCI	GTSAC	4 cut(s)	57, 491, 1184, 1725
NsbI	TGCGCA	1 cut(s)	1706
NspI	RCATGY	1 cut(s)	670
PagI	TCATGA	1 cut(s)	1128
PaqCI	CACCTGC	1 cut(s)	70
PciSI	GCTCTTC	1 cut(s)	1375
PcsI	WCGNNNNNNNCGW	1 cut(s)	97
PctI	GAATGC	1 cut(s)	767
PfeI	GAWTC	9 cut(s)	102, 184, 472, 909, 1039, 1240, 1414, 1654, 1684
PinAI	ACCGGT	1 cut(s)	1722
PkrI	GCNGC	1 cut(s)	553
PleI	GAGTC	2 cut(s)	269, 1472
PluTI	GGCGCC	1 cut(s)	614
PpsI	GAGTC	2 cut(s)	269, 1472
Ppu21I	YACGTR	1 cut(s)	434
PpuMI	RGGWCCY	1 cut(s)	160
Psp1406I	AACGTT	1 cut(s)	1785
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	1 cut(s)	1801
PspEI	GGTNACC	1 cut(s)	837
PspGI	CCWGG	1 cut(s)	1801
PspN4I	GGNNCC	4 cut(s)	127, 419, 612, 1194
PspPI	GGNCC	3 cut(s)	160, 247, 417
PspPPI	RGGWCCY	1 cut(s)	160
PstNI	CAGNNNCTG	1 cut(s)	1188
PsuI	RGATCY	3 cut(s)	79, 426, 1400
RsaI	GTAC	5 cut(s)	371, 383, 568, 595, 623
RsaNI	GTAC	5 cut(s)	370, 382, 567, 594, 622
RseI	CAYNNNNRTG	4 cut(s)	341, 671, 1016, 1700
SapI	GCTCTTC	1 cut(s)	1375
SaqAI	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1761
SatI	GCNGC	1 cut(s)	552
Sau96I	GGNCC	3 cut(s)	160, 247, 417
SchI	GAGTC	2 cut(s)	269, 1472
ScrFI	CCNGG	3 cut(s)	421, 1245, 1803
SduI	GDGCHC	3 cut(s)	716, 1265, 1370
SfaNI	GCATC	1 cut(s)	193
SfcI	CTRYAG	1 cut(s)	703
SfoI	GGCGCC	1 cut(s)	612
SinI	GGWCC	3 cut(s)	160, 247, 417
SmiMI	CAYNNNNRTG	4 cut(s)	341, 671, 1016, 1700
SmlI	CTYRAG	2 cut(s)	257, 485
SmoI	CTYRAG	2 cut(s)	257, 485
SsiI	CCGC	4 cut(s)	950, 956, 987, 1753
SspDI	GGCGCC	1 cut(s)	610
SspI	AATATT	1 cut(s)	397
SspMI	CTAG	7 cut(s)	518, 644, 872, 893, 1101, 1628, 1810
StyD4I	CCNGG	3 cut(s)	419, 1243, 1801
StyI	CCWWGG	1 cut(s)	39
TaaI	ACNGT	6 cut(s)	57, 139, 584, 1069, 1433, 1768
TaiI	ACGT	5 cut(s)	72, 436, 1539, 1704, 1788
TaqI	TCGA	5 cut(s)	91, 306, 319, 1476, 1698
TatI	WGTACW	1 cut(s)	369
TfiI	GAWTC	9 cut(s)	102, 184, 472, 909, 1039, 1240, 1414, 1654, 1684
Tru1I	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1761
Tru9I	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1761
TscAI	CASTG	8 cut(s)	411, 589, 951, 1033, 1144, 1189, 1412, 1734
TseFI	GTSAC	4 cut(s)	57, 491, 1184, 1725
TseI	GCWGC	1 cut(s)	551
Tsp45I	GTSAC	4 cut(s)	57, 491, 1184, 1725
TspGWI	ACGGA	2 cut(s)	111, 1172
TspRI	CASTG	8 cut(s)	411, 589, 951, 1033, 1144, 1189, 1412, 1734
VpaK11BI	GGWCC	3 cut(s)	160, 247, 417
XapI	RAATTY	4 cut(s)	602, 729, 1381, 1600
XceI	RCATGY	1 cut(s)	670
XspI	CTAG	7 cut(s)	518, 644, 872, 893, 1101, 1628, 1810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.