Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009014

Physical Location & Seq

Genomic Coordinates Reverse (-)

26752563 .. 26756573

Gene Structure 4011 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1186000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGAACAAGAACAACAGCCGGATCGGAAGCAGGTCTGCCAATGGAGGCAGGCCACAATCACCTGCTTACGTTGAAACTGATCCATCTGGTCGCTATGGACGTTTCAGGGAAATGCTTGGCAAAGGAGCCATGAAGACAGTTTACAAGGCATTTGATGAGGTCCTCGGAATGGAGGTGGCTTGGAATCAAGTCAAGCTTAATGATGTCTTTACTTCACCGGACGAACTTCAGCGCCTATACTCCGAGGTTCACCTCCTCAAGAACCTCAATCATGACTCCATCATCCAATACTACGCATCTTGGATCGATGTTAATCGCCGAACCTTCAACTTCATCACCGAAATGTTCACCTCCGGCACTCTCAGAGAGTACAGACAGAAATACCAGCAAGTGAATATTGGAGCAGTGAAAAATTGGTCCCGACAGATCTTGCGTGGTCTGGCTTATTTGCACAAACTTGATCCACCTGTGATTCACAGAGACCTCAAGTGTGACAACATCTTTGTTAATGGCCACCTAGGGCAAGTCAAGATTGGTGATTTGGGCTTGGCTGCTATTCTTCGTGGTACGCAACACGCCCACAGTGTCATAGGTACACCAGAATTTATGGCGCCTGAATTGTACGATGAGGAATACAATGAGCTGGTAGACATATATGCCTTTGGCATGTGTGTGTTGGAAATGCTTACTTCAGAATATCCCTATAGCGAGTGCTCAAATCCTGCCCAAATTTACAAGAAAGTTACTTCAGGGAAGCTACCAAACGCATTCTACAATATTGAAGATTTGGAGGCTCAACGATTTGTAGGGAAGTGTCTGGAGAATGTTTCAAAGAGGTTGCCGGCACATGAGCTATTGCTAGACCCTTTTCTAGCCTCTGATGATGGAGAGCTAGCGTCTTCCCCAAGAATCCCATTTCTGAAGTTAATTCCTGATAATGATCCAGTCGTGGAGGCAGAGGATGAGGAGGATGAGCTACATTTTGCCGCTGAGGTTGATTTGGAAAGAAGCACAAACATGATCATCACTGGAAAAATGAATCTAGAGGATGATACTATTACTCTCAAAGTTCGAATTTCTGACCTGGACGGGAAAAATGTTAGGAACATATACTTTCCCTTCGACATTTTGAACGACACTGCGATTGAAGTAGCAACGGAGATGGTGAAAGAATTAGAAATCAATGACTGGGAACCGCCAGAGATCGCAGGGATGATAGAAAACGAGATATCTTCTTTGATTGCGGGTAGTAAGAAATGGGGCTCACCCCGAGATTATCATCCTCATCAGCACAGCTTTAACTATGAAGAAGAAGATGATAATAATAATGGTGGTATCACCCATCATCCTTTCTACGCATTCTCTTCCTGCTCTTCATCCCAAAATTCTCTTCATGCTTTGAGCTTTCACTGCAAGAATCATGATTCGCTTCAAGGTGATCTGTTTATGAATGATGATGTGAGTTCTCAGAGTTCATTCGACTCCTGCAACTACTCCAACATAAGTTACTACTCAGGCAATGAAGATGATCTCCTTGACATGAGCTTCGGAAAGACTACTCACAAATCTACTCGGTTTTGTCCTTCAAAAAGCAGGGTTGCAAATACTTACAAACATTGCAATTCACAACTAGATAACCACAGACCTCAAAAATTGAATCATCATCATGAAAGAAAATTACCCAAAATTCAATCACTCATTGACGTGCACAGCCAATTGTTGCACAGGTCACTGGTGGAGGAGGTAAACAAGAGGTGGTTGTTTAAGACTGTTGGGACAATTGAGAACGTTGGGTTTCAAACACCAGGAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

605

Amino Acids

68.82

Weight (kDa)

5.45

Isoelectric Point (pI)

51.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	35 - 274	4.8e-30	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	37 - 291	5.6e-46	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009014)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51630 AT3G51630
fragaria_vesca	FvH4_6g10120
malus_domestica	MD04G1172700.v1.1 MD12G1186000.v1.1
prunus_persica	Prupe.4G073500_v2.0.a1 Prupe.6G291500_v2.0.a1
pyrus_communis	pycom04g15260 pycom12g17460
rosa_chinensis	RchiOBHm_Chr3g0460821
rosa_laevigata	RLG00000024978
rosa_multiflora	Rmu_sc0007678.1_g000013
rosa_roxburghii	Rroxscaffold_6G00419190
rosa_rugosa	Rorug03G0046600 Rorug03G0046700 Rorug03G0046800 Rorug03G0046800
rosa_samantha	Rh3AG103700 Rh3BG107400 Rh3CG109300 Rh3DG109200
rosa_wichuraiana	Rw3G008830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	70
Acc36I	ACCTGC	2 cut(s)	21, 70
AccB1I	GGYRCC	1 cut(s)	610
AccI	GTMKAC	1 cut(s)	648
AciI	CCGC	3 cut(s)	987, 1196, 1244
AclI	AACGTT	1 cut(s)	1788
AclWI	GGATC	5 cut(s)	29, 74, 311, 455, 935
AcoI	YGGCCR	1 cut(s)	511
AcsI	RAATTY	5 cut(s)	602, 729, 1074, 1384, 1686
AcuI	CTGAAG	4 cut(s)	212, 675, 732, 941
AcyI	GRCGYC	1 cut(s)	611
AfaI	GTAC	4 cut(s)	371, 568, 595, 623
AfiI	CCNNNNNNNGG	1 cut(s)	169
AjiI	CACGTC	1 cut(s)	1705
AjnI	CCWGG	2 cut(s)	1083, 1804
AjuI	GAANNNNNNNTTGG	2 cut(s)	1374, 1406
Alw21I	GWGCWC	2 cut(s)	716, 1710
Alw26I	GTCTC	1 cut(s)	474
Alw44I	GTGCAC	1 cut(s)	1706
AlwI	GGATC	5 cut(s)	29, 74, 311, 455, 935
Ama87I	CYCGRG	1 cut(s)	1269
AoxI	GGCC	2 cut(s)	50, 511
ApaLI	GTGCAC	1 cut(s)	1706
ApeKI	GCWGC	1 cut(s)	551
ApoI	RAATTY	5 cut(s)	602, 729, 1074, 1384, 1686
ArsI	GACNNNNNNTTYG	4 cut(s)	485, 517, 1529, 1561
AspA2I	CCTAGG	1 cut(s)	517
AspLEI	GCGC	2 cut(s)	234, 613
AspS9I	GGNCC	2 cut(s)	160, 417
AsuII	TTCGAA	1 cut(s)	1072
AsuNHI	GCTAGC	1 cut(s)	892
AvaI	CYCGRG	1 cut(s)	1269
AvaII	GGWCC	2 cut(s)	160, 417
AvrII	CCTAGG	1 cut(s)	517
BaeGI	GKGCMC	1 cut(s)	1710
BalI	TGGCCA	1 cut(s)	513
BanI	GGYRCC	1 cut(s)	610
BanII	GRGCYC	1 cut(s)	1265
BarI	GAAGNNNNNNTAC	2 cut(s)	125, 157
BbsI	GAAGAC	2 cut(s)	140, 891
Bbv12I	GWGCWC	2 cut(s)	716, 1710
BbvCI	CCTCAGC	1 cut(s)	990
BbvI	GCAGC	1 cut(s)	538
BccI	CCATC	5 cut(s)	91, 287, 878, 1156, 1350
BciT130I	CCWGG	2 cut(s)	1085, 1806
BclI	TGATCA	1 cut(s)	1020
BcoDI	GTCTC	1 cut(s)	474
BfaI	CTAG	7 cut(s)	518, 860, 872, 893, 1043, 1631, 1813
BfmI	CTRYAG	1 cut(s)	703
BfoI	RGCGCY	2 cut(s)	235, 614
BfuAI	ACCTGC	2 cut(s)	21, 70
BglII	AGATCT	1 cut(s)	426
BisI	GCNGC	2 cut(s)	552, 987
BlnI	CCTAGG	1 cut(s)	517
BlsI	GCNGC	2 cut(s)	553, 988
Bme1390I	CCNGG	2 cut(s)	1085, 1806
Bme18I	GGWCC	2 cut(s)	160, 417
BmeT110I	CYCGRG	1 cut(s)	1269
BmgBI	CACGTC	1 cut(s)	1705
BmgT120I	GGNCC	2 cut(s)	160, 417
BmiI	GGNNCC	4 cut(s)	127, 419, 612, 1194
BmrFI	CCNGG	2 cut(s)	1085, 1806
BmrI	ACTGGG	1 cut(s)	1198
BmsI	GCATC	1 cut(s)	305
BmtI	GCTAGC	1 cut(s)	896
BmuI	ACTGGG	1 cut(s)	1198
BpiI	GAAGAC	2 cut(s)	140, 891
BpmI	CTGGAG	1 cut(s)	839
Bpu10I	CCTNAGC	1 cut(s)	990
Bpu14I	TTCGAA	1 cut(s)	1072
BpuEI	CTTGAG	2 cut(s)	242, 470
Bsa29I	ATCGAT	1 cut(s)	306
BsaBI	GATNNNNATC	3 cut(s)	312, 939, 1278
BsaHI	GRCGYC	1 cut(s)	611
BsaI	GGTCTC	1 cut(s)	474
BsaJI	CCNNGG	3 cut(s)	163, 243, 517
BsaWI	WCCGGW	1 cut(s)	217
Bsc4I	CCNNNNNNNGG	1 cut(s)	169
Bse118I	RCCGGY	1 cut(s)	841
Bse1I	ACTGG	4 cut(s)	944, 1033, 1193, 1737
Bse3DI	GCAATG	2 cut(s)	1525, 1615
Bse8I	GATNNNNATC	3 cut(s)	312, 939, 1278
BseBI	CCWGG	2 cut(s)	1085, 1806
BseCI	ATCGAT	1 cut(s)	306
BseDI	CCNNGG	3 cut(s)	163, 243, 517
BseGI	GGATG	8 cut(s)	282, 967, 976, 1054, 1218, 1279, 1345, 1376
BseJI	GATNNNNATC	3 cut(s)	312, 939, 1278
BseLI	CCNNNNNNNGG	1 cut(s)	169
BseMI	GCAATG	2 cut(s)	1525, 1615
BseMII	CTCAG	4 cut(s)	376, 981, 1481, 1527
BseNI	ACTGG	4 cut(s)	944, 1033, 1193, 1737
BseRI	GAGGAG	3 cut(s)	245, 980, 1754
BseSI	GKGCMC	1 cut(s)	1710
BseXI	GCAGC	1 cut(s)	538
BshFI	GGCC	2 cut(s)	52, 513
BshNI	GGYRCC	1 cut(s)	610
BshVI	ATCGAT	1 cut(s)	306
BsiHKAI	GWGCWC	2 cut(s)	716, 1710
BsiHKCI	CYCGRG	1 cut(s)	1269
BsiSI	CCGG	4 cut(s)	19, 218, 354, 842
BslFI	GGGAC	2 cut(s)	403, 1789
BslI	CCNNNNNNNGG	1 cut(s)	169
BsmAI	GTCTC	1 cut(s)	474
BsmFI	GGGAC	2 cut(s)	403, 1789
BsmI	GAATGC	2 cut(s)	767, 1358
BsnI	GGCC	2 cut(s)	52, 513
Bso31I	GGTCTC	1 cut(s)	474
BsoBI	CYCGRG	1 cut(s)	1269
Bsp119I	TTCGAA	1 cut(s)	1072
Bsp1286I	GDGCHC	3 cut(s)	716, 1265, 1710
BspACI	CCGC	3 cut(s)	987, 1196, 1244
BspANI	GGCC	2 cut(s)	52, 513
BspCNI	CTCAG	4 cut(s)	375, 982, 1480, 1526
BspDI	ATCGAT	1 cut(s)	306
BspHI	TCATGA	3 cut(s)	271, 1420, 1666
BspLI	GGNNCC	4 cut(s)	127, 419, 612, 1194
BspMI	ACCTGC	2 cut(s)	21, 70
BspOI	GCTAGC	1 cut(s)	896
BspPI	GGATC	5 cut(s)	29, 74, 311, 455, 935
BspQI	GCTCTTC	1 cut(s)	1378
BspT104I	TTCGAA	1 cut(s)	1072
BspT107I	GGYRCC	1 cut(s)	610
BspTNI	GGTCTC	1 cut(s)	474
BsrDI	GCAATG	2 cut(s)	1525, 1615
BsrFI	RCCGGY	1 cut(s)	841
BsrI	ACTGG	4 cut(s)	944, 1033, 1193, 1737
BssAI	RCCGGY	1 cut(s)	841
BssECI	CCNNGG	3 cut(s)	163, 243, 517
BssNI	GRCGYC	1 cut(s)	611
BssT1I	CCWWGG	1 cut(s)	517
Bst2UI	CCWGG	2 cut(s)	1085, 1806
Bst4CI	ACNGT	3 cut(s)	139, 584, 1771
Bst6I	CTCTTC	3 cut(s)	1369, 1378, 1395
BstACI	GRCGYC	1 cut(s)	611
BstBI	TTCGAA	1 cut(s)	1072
BstC8I	GCNNGC	3 cut(s)	50, 843, 894
BstDEI	CTNAG	4 cut(s)	362, 990, 1467, 1513
BstF5I	GGATG	8 cut(s)	282, 967, 976, 1054, 1218, 1279, 1345, 1376
BstH2I	RGCGCY	2 cut(s)	235, 614
BstHHI	GCGC	2 cut(s)	234, 613
BstMAI	GTCTC	1 cut(s)	474
BstMWI	GCNNNNNNNGC	1 cut(s)	1410
BstNI	CCWGG	2 cut(s)	1085, 1806
BstNSI	RCATGY	1 cut(s)	670
BstSCI	CCNGG	2 cut(s)	1083, 1804
BstSFI	CTRYAG	1 cut(s)	703
BstSLI	GKGCMC	1 cut(s)	1710
BstV1I	GCAGC	1 cut(s)	538
BstV2I	GAAGAC	2 cut(s)	140, 891
BstX2I	RGATCY	1 cut(s)	426
BstYI	RGATCY	1 cut(s)	426
Bsu15I	ATCGAT	1 cut(s)	306
BsuRI	GGCC	2 cut(s)	52, 513
BsuTUI	ATCGAT	1 cut(s)	306
BtrI	CACGTC	1 cut(s)	1705
BtsCI	GGATG	8 cut(s)	282, 967, 976, 1054, 1218, 1279, 1345, 1376
BtsI	GCAGTG	3 cut(s)	411, 1137, 1408
BtsIMutI	CAGTG	6 cut(s)	411, 589, 1026, 1137, 1408, 1730
BveI	ACCTGC	2 cut(s)	21, 70
Cac8I	GCNNGC	3 cut(s)	50, 843, 894
CciI	TCATGA	3 cut(s)	271, 1420, 1666
CfoI	GCGC	2 cut(s)	234, 613
Cfr10I	RCCGGY	1 cut(s)	841
Cfr13I	GGNCC	2 cut(s)	160, 417
ClaI	ATCGAT	1 cut(s)	306
CseI	GACGC	1 cut(s)	885
Csp6I	GTAC	4 cut(s)	370, 567, 594, 622
CviAII	CATG	9 cut(s)	130, 272, 667, 848, 1018, 1394, 1421, 1540, 1667
CviQI	GTAC	4 cut(s)	370, 567, 594, 622
DdeI	CTNAG	4 cut(s)	362, 990, 1467, 1513
DinI	GGCGCC	1 cut(s)	612
EaeI	YGGCCR	1 cut(s)	511
Eam1104I	CTCTTC	3 cut(s)	1369, 1378, 1395
EarI	CTCTTC	3 cut(s)	1369, 1378, 1395
Eco130I	CCWWGG	1 cut(s)	517
Eco24I	GRGCYC	1 cut(s)	1265
Eco31I	GGTCTC	1 cut(s)	474
Eco32I	GATATC	1 cut(s)	1230
Eco47I	GGWCC	2 cut(s)	160, 417
Eco57I	CTGAAG	4 cut(s)	212, 675, 732, 941
Eco88I	CYCGRG	1 cut(s)	1269
EcoO109I	RGGNCCY	1 cut(s)	160
EcoRII	CCWGG	2 cut(s)	1083, 1804
EcoRV	GATATC	1 cut(s)	1230
EcoT14I	CCWWGG	1 cut(s)	517
EcoT38I	GRGCYC	1 cut(s)	1265
EgeI	GGCGCC	1 cut(s)	612
EheI	GGCGCC	1 cut(s)	612
ErhI	CCWWGG	1 cut(s)	517
FaeI	CATG	9 cut(s)	133, 275, 670, 851, 1021, 1397, 1424, 1543, 1670
FalI	AAGNNNNNCTT	2 cut(s)	180, 212
FaqI	GGGAC	2 cut(s)	403, 1789
FatI	CATG	9 cut(s)	129, 271, 666, 847, 1017, 1393, 1420, 1539, 1666
FauI	CCCGC	1 cut(s)	1237
FbaI	TGATCA	1 cut(s)	1020
FblI	GTMKAC	1 cut(s)	648
Fnu4HI	GCNGC	2 cut(s)	552, 987
FokI	GGATG	8 cut(s)	269, 974, 983, 1061, 1225, 1266, 1332, 1363
FriOI	GRGCYC	1 cut(s)	1265
Fsp4HI	GCNGC	2 cut(s)	552, 987
FspBI	CTAG	7 cut(s)	518, 860, 872, 893, 1043, 1631, 1813
GlaI	GCGC	2 cut(s)	233, 612
GluI	GCNGC	2 cut(s)	552, 987
GsuI	CTGGAG	1 cut(s)	839
HaeII	RGCGCY	2 cut(s)	235, 614
HaeIII	GGCC	2 cut(s)	52, 513
HapII	CCGG	4 cut(s)	19, 218, 354, 842
HgaI	GACGC	1 cut(s)	885
HhaI	GCGC	2 cut(s)	234, 613
Hin1I	GRCGYC	1 cut(s)	611
Hin1II	CATG	9 cut(s)	133, 275, 670, 851, 1021, 1397, 1424, 1543, 1670
Hin6I	GCGC	2 cut(s)	232, 611
HinP1I	GCGC	2 cut(s)	232, 611
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	9 cut(s)	184, 275, 472, 909, 1039, 1417, 1424, 1481, 1657
HpaII	CCGG	4 cut(s)	19, 218, 354, 842
Hpy166II	GTNNAC	7 cut(s)	142, 250, 348, 596, 649, 1708, 1747
Hpy188III	TCNNGA	9 cut(s)	259, 272, 420, 529, 818, 932, 1043, 1421, 1667
Hpy8I	GTNNAC	7 cut(s)	142, 250, 348, 596, 649, 1708, 1747
HpyAV	CCTTC	3 cut(s)	334, 1129, 1593
HpyCH4III	ACNGT	3 cut(s)	139, 584, 1771
HpyCH4IV	ACGT	4 cut(s)	69, 100, 1704, 1788
HpyCH4V	TGCA	7 cut(s)	451, 1413, 1488, 1601, 1620, 1708, 1723
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1410
HpyF3I	CTNAG	4 cut(s)	362, 990, 1467, 1513
HpySE526I	ACGT	4 cut(s)	69, 100, 1704, 1788
Hsp92I	GRCGYC	1 cut(s)	611
Hsp92II	CATG	9 cut(s)	133, 275, 670, 851, 1021, 1397, 1424, 1543, 1670
HspAI	GCGC	2 cut(s)	232, 611
KasI	GGCGCC	1 cut(s)	610
KroI	GCCGGC	1 cut(s)	841
KroNI	GCCGGC	1 cut(s)	843
Ksp22I	TGATCA	1 cut(s)	1020
LguI	GCTCTTC	1 cut(s)	1378
LmnI	GCTCC	2 cut(s)	125, 401
Lsp1109I	GCAGC	1 cut(s)	538
LweI	GCATC	1 cut(s)	305
MaeI	CTAG	7 cut(s)	518, 860, 872, 893, 1043, 1631, 1813
MaeII	ACGT	4 cut(s)	69, 100, 1704, 1788
MaeIII	GTNAC	4 cut(s)	491, 742, 1505, 1728
MfeI	CAATTG	2 cut(s)	1715, 1779
MflI	RGATCY	1 cut(s)	426
MhlI	GDGCHC	3 cut(s)	716, 1265, 1710
MlsI	TGGCCA	1 cut(s)	513
MluNI	TGGCCA	1 cut(s)	513
Mly113I	GGCGCC	1 cut(s)	611
MlyI	GAGTC	2 cut(s)	269, 1475
MmeI	TCCRAC	2 cut(s)	657, 1521
Mox20I	TGGCCA	1 cut(s)	513
MroNI	GCCGGC	1 cut(s)	841
MscI	TGGCCA	1 cut(s)	513
MseI	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1764
MslI	CAYNNNNRTG	5 cut(s)	341, 671, 1016, 1665, 1703
Msp20I	TGGCCA	1 cut(s)	513
MspA1I	CMGCKG	1 cut(s)	989
MspI	CCGG	4 cut(s)	19, 218, 354, 842
MspR9I	CCNGG	2 cut(s)	1085, 1806
MunI	CAATTG	2 cut(s)	1715, 1779
Mva1269I	GAATGC	2 cut(s)	767, 1358
MvaI	CCWGG	2 cut(s)	1085, 1806
MwoI	GCNNNNNNNGC	1 cut(s)	1410
NaeI	GCCGGC	1 cut(s)	843
NarI	GGCGCC	1 cut(s)	611
NgoMIV	GCCGGC	1 cut(s)	841
NheI	GCTAGC	1 cut(s)	892
NlaIII	CATG	9 cut(s)	133, 275, 670, 851, 1021, 1397, 1424, 1543, 1670
NlaIV	GGNNCC	4 cut(s)	127, 419, 612, 1194
NmuCI	GTSAC	2 cut(s)	491, 1728
NspI	RCATGY	1 cut(s)	670
NspV	TTCGAA	1 cut(s)	1072
PagI	TCATGA	3 cut(s)	271, 1420, 1666
PaqCI	CACCTGC	1 cut(s)	70
PciSI	GCTCTTC	1 cut(s)	1378
PcsI	WCGNNNNNNNCGW	1 cut(s)	97
PctI	GAATGC	2 cut(s)	767, 1358
PdiI	GCCGGC	1 cut(s)	843
PfeI	GAWTC	7 cut(s)	184, 472, 909, 1039, 1417, 1424, 1657
PkrI	GCNGC	2 cut(s)	553, 988
PleI	GAGTC	2 cut(s)	269, 1475
PluTI	GGCGCC	1 cut(s)	614
PpsI	GAGTC	2 cut(s)	269, 1475
PpuMI	RGGWCCY	1 cut(s)	160
Psp1406I	AACGTT	1 cut(s)	1788
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	2 cut(s)	1083, 1804
PspGI	CCWGG	2 cut(s)	1083, 1804
PspN4I	GGNNCC	4 cut(s)	127, 419, 612, 1194
PspPI	GGNCC	2 cut(s)	160, 417
PspPPI	RGGWCCY	1 cut(s)	160
PsuI	RGATCY	1 cut(s)	426
RsaI	GTAC	4 cut(s)	371, 568, 595, 623
RsaNI	GTAC	4 cut(s)	370, 567, 594, 622
RseI	CAYNNNNRTG	5 cut(s)	341, 671, 1016, 1665, 1703
SapI	GCTCTTC	1 cut(s)	1378
SaqAI	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1764
SatI	GCNGC	2 cut(s)	552, 987
Sau96I	GGNCC	2 cut(s)	160, 417
SchI	GAGTC	2 cut(s)	269, 1475
ScrFI	CCNGG	2 cut(s)	1085, 1806
SduI	GDGCHC	3 cut(s)	716, 1265, 1710
SfaNI	GCATC	1 cut(s)	305
SfcI	CTRYAG	1 cut(s)	703
SfoI	GGCGCC	1 cut(s)	612
SfuI	TTCGAA	1 cut(s)	1072
SinI	GGWCC	2 cut(s)	160, 417
SmiMI	CAYNNNNRTG	5 cut(s)	341, 671, 1016, 1665, 1703
SmlI	CTYRAG	2 cut(s)	257, 485
SmoI	CTYRAG	2 cut(s)	257, 485
SsiI	CCGC	3 cut(s)	987, 1196, 1244
SspDI	GGCGCC	1 cut(s)	610
SspI	AATATT	2 cut(s)	397, 778
SspMI	CTAG	7 cut(s)	518, 860, 872, 893, 1043, 1631, 1813
StyD4I	CCNGG	2 cut(s)	1083, 1804
StyI	CCWWGG	1 cut(s)	517
TaaI	ACNGT	3 cut(s)	139, 584, 1771
TaiI	ACGT	4 cut(s)	72, 103, 1707, 1791
TaqI	TCGA	4 cut(s)	306, 1072, 1122, 1479
TatI	WGTACW	1 cut(s)	369
TauI	GCSGC	1 cut(s)	989
TfiI	GAWTC	7 cut(s)	184, 472, 909, 1039, 1417, 1424, 1657
Tru1I	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1764
Tru9I	TTAA	6 cut(s)	198, 312, 507, 926, 1299, 1764
TscAI	CASTG	6 cut(s)	411, 589, 1033, 1144, 1415, 1737
TseFI	GTSAC	2 cut(s)	491, 1728
TseI	GCWGC	1 cut(s)	551
Tsp45I	GTSAC	2 cut(s)	491, 1728
TspGWI	ACGGA	1 cut(s)	1172
TspRI	CASTG	6 cut(s)	411, 589, 1033, 1144, 1415, 1737
VneI	GTGCAC	1 cut(s)	1706
VpaK11BI	GGWCC	2 cut(s)	160, 417
XapI	RAATTY	5 cut(s)	602, 729, 1074, 1384, 1686
XbaI	TCTAGA	1 cut(s)	1042
XceI	RCATGY	1 cut(s)	670
XmaJI	CCTAGG	1 cut(s)	517
XmiI	GTMKAC	1 cut(s)	648
XspI	CTAG	7 cut(s)	518, 860, 872, 893, 1043, 1631, 1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.